AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042663

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042663.t1 MTR_5g054300 94.215 363 19 2 1 363 1 361 0.0 687
MS.gene042663.t1 MTR_1g093080 60.172 349 109 13 10 333 1 344 5.14e-125 365
MS.gene042663.t1 MTR_1g093080 59.696 263 81 8 10 249 1 261 2.02e-90 273
MS.gene042663.t1 MTR_1g093080 56.540 237 78 8 10 223 1 235 6.30e-73 227
MS.gene042663.t1 MTR_4g113140 36.915 363 154 15 4 310 7 350 3.04e-51 176
MS.gene042663.t1 MTR_4g086835 34.783 345 144 13 16 296 41 368 1.30e-42 153
MS.gene042663.t1 MTR_4g086835 34.783 345 143 14 16 296 41 367 9.40e-41 148
MS.gene042663.t1 MTR_5g017980 38.492 252 106 8 19 260 42 254 3.85e-36 134
MS.gene042663.t1 MTR_5g017980 38.492 252 104 9 19 260 42 252 2.60e-34 129
MS.gene042663.t1 MTR_5g017980 38.492 252 105 9 19 260 42 253 3.48e-34 129
MS.gene042663.t1 MTR_5g014040 49.333 75 34 3 193 266 304 375 1.08e-13 72.4
MS.gene042663.t1 MTR_4g098870 44.211 95 41 3 173 256 273 366 1.49e-13 72.0
MS.gene042663.t1 MTR_4g098870 44.211 95 41 3 173 256 293 386 1.52e-13 72.0
MS.gene042663.t1 MTR_4g098870 44.211 95 41 3 173 256 312 405 1.74e-13 72.0
MS.gene042663.t1 MTR_4g098870 44.211 95 41 3 173 256 273 366 1.77e-13 72.0
MS.gene042663.t1 MTR_4g098870 44.211 95 41 3 173 256 293 386 1.85e-13 72.0
MS.gene042663.t1 MTR_1g080330 58.929 56 23 0 197 252 173 228 2.40e-13 70.1
MS.gene042663.t1 MTR_7g098250 45.783 83 45 0 175 257 238 320 2.49e-13 71.2
MS.gene042663.t1 MTR_3g106220 33.600 125 77 3 188 307 186 309 3.50e-13 71.2
MS.gene042663.t1 MTR_1g032570 45.333 75 40 1 193 267 194 267 4.38e-13 70.9
MS.gene042663.t1 MTR_1g080330 58.929 56 23 0 197 252 173 228 4.60e-13 70.1
MS.gene042663.t1 MTR_1g032570 45.333 75 40 1 193 267 125 198 4.62e-13 70.5
MS.gene042663.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 4.69e-13 69.3
MS.gene042663.t1 MTR_2g027860 58.182 55 23 0 199 253 49 103 5.52e-13 69.3
MS.gene042663.t1 MTR_4g121020 41.053 95 43 3 198 290 214 297 5.59e-13 70.5
MS.gene042663.t1 MTR_4g121020 41.053 95 43 3 198 290 214 297 5.74e-13 70.5
MS.gene042663.t1 MTR_4g121020 39.394 99 47 3 198 294 110 197 6.72e-13 70.1
MS.gene042663.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 9.14e-13 68.9
MS.gene042663.t1 MTR_3g102600 43.038 79 41 2 203 278 87 164 9.64e-13 66.2
MS.gene042663.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 1.01e-12 68.9
MS.gene042663.t1 MTR_7g089010 44.156 77 42 1 181 257 73 148 1.08e-12 68.2
MS.gene042663.t1 MTR_3g102600 43.038 79 41 2 203 278 136 213 1.19e-12 67.0
MS.gene042663.t1 MTR_7g088070 55.172 58 26 0 196 253 47 104 1.23e-12 67.8
MS.gene042663.t1 MTR_7g088070 55.172 58 26 0 196 253 47 104 1.25e-12 67.8
MS.gene042663.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 1.49e-12 68.9
MS.gene042663.t1 MTR_2g450070 36.207 116 66 4 197 310 211 320 1.69e-12 68.9
MS.gene042663.t1 MTR_2g450070 36.207 116 66 4 197 310 211 320 1.82e-12 68.9
MS.gene042663.t1 MTR_2g023580 41.573 89 40 2 164 252 32 108 2.48e-12 66.2
MS.gene042663.t1 MTR_2g034960 34.746 118 71 3 198 310 210 326 3.44e-12 68.2
MS.gene042663.t1 MTR_2g086450 41.379 87 46 1 172 253 13 99 3.66e-12 67.4
MS.gene042663.t1 MTR_2g027800 45.205 73 40 0 199 271 39 111 4.52e-12 66.6
MS.gene042663.t1 MTR_7g117705 50.820 61 29 1 196 256 188 247 5.51e-12 67.0
MS.gene042663.t1 MTR_7g115530 58.182 55 23 0 197 251 216 270 7.20e-12 65.5
MS.gene042663.t1 MTR_1g013170 50.820 61 29 1 197 257 189 248 7.57e-12 66.6
MS.gene042663.t1 MTR_5g041350 54.545 55 25 0 199 253 15 69 8.17e-12 66.2
MS.gene042663.t1 MTR_5g014040 46.250 80 34 4 193 266 264 340 8.58e-12 66.6
MS.gene042663.t1 MTR_4g064730 42.105 76 43 1 198 273 130 204 8.72e-12 65.9
MS.gene042663.t1 MTR_4g021790 47.561 82 34 3 185 258 146 226 8.84e-12 65.5
MS.gene042663.t1 MTR_6g444980 58.182 55 23 0 199 253 35 89 1.01e-11 65.9
MS.gene042663.t1 MTR_5g014040 46.250 80 34 4 193 266 304 380 1.06e-11 66.6
MS.gene042663.t1 MTR_6g444980 58.182 55 23 0 199 253 35 89 1.13e-11 65.9
MS.gene042663.t1 MTR_1g053830 47.170 53 28 0 201 253 20 72 1.21e-11 61.2
MS.gene042663.t1 MTR_2g027800 56.364 55 24 0 199 253 39 93 1.56e-11 65.1
MS.gene042663.t1 MTR_1g053835 41.772 79 37 1 184 253 234 312 2.47e-11 65.1
MS.gene042663.t1 MTR_2g090675 34.951 103 59 2 154 256 123 217 2.66e-11 65.1
MS.gene042663.t1 MTR_4g131580 49.180 61 30 1 197 257 198 257 3.07e-11 65.1
MS.gene042663.t1 MTR_7g115530 58.491 53 22 0 197 249 216 268 3.74e-11 63.5
MS.gene042663.t1 MTR_7g069660 55.102 49 22 0 204 252 76 124 5.52e-11 63.5
MS.gene042663.t1 MTR_4g081710 52.727 55 26 0 199 253 17 71 6.34e-11 63.5
MS.gene042663.t1 MTR_1g112370 55.102 49 22 0 204 252 27 75 7.08e-11 63.2
MS.gene042663.t1 MTR_4g078810 45.161 62 33 1 195 256 154 214 8.06e-11 63.5
MS.gene042663.t1 MTR_6g032990 50.909 55 27 0 199 253 42 96 9.30e-11 63.2
MS.gene042663.t1 MTR_1g090670 54.545 55 25 0 199 253 44 98 9.50e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042663.t1 AT1G25550 47.337 338 138 11 1 320 1 316 5.28e-83 256
MS.gene042663.t1 AT1G68670 46.686 347 127 13 1 313 2 324 1.25e-76 240
MS.gene042663.t1 AT1G25550 52.273 264 106 8 1 249 1 259 1.90e-72 227
MS.gene042663.t1 AT3G25790 41.828 361 160 12 19 359 20 350 3.15e-62 203
MS.gene042663.t1 AT1G13300 44.718 284 104 9 20 285 20 268 7.62e-61 199
MS.gene042663.t1 AT3G25790 43.952 248 101 6 19 249 20 246 2.57e-47 161
MS.gene042663.t1 AT2G03500 33.934 333 159 9 1 294 18 328 3.59e-44 158
MS.gene042663.t1 AT4G37180 33.829 269 123 7 11 260 39 271 1.28e-31 122
MS.gene042663.t1 AT4G37180 33.086 269 132 7 11 260 39 278 1.39e-30 120
MS.gene042663.t1 AT4G37180 33.333 258 117 7 11 249 39 260 6.16e-27 108
MS.gene042663.t1 AT1G49560 31.900 279 138 10 16 279 27 268 2.66e-25 105
MS.gene042663.t1 AT1G49560 31.855 248 123 8 16 249 27 242 6.15e-21 91.3
MS.gene042663.t1 AT2G25180 37.600 125 63 4 197 307 193 316 1.10e-13 72.8
MS.gene042663.t1 AT5G58080 47.222 72 34 2 189 257 181 251 1.64e-13 72.4
MS.gene042663.t1 AT5G18240 42.857 91 46 2 168 253 10 99 2.77e-13 70.9
MS.gene042663.t1 AT5G18240 42.857 91 46 2 168 253 10 99 2.77e-13 70.9
MS.gene042663.t1 AT5G18240 42.857 91 46 2 168 253 10 99 3.29e-13 70.9
MS.gene042663.t1 AT5G05090 44.872 78 42 1 180 257 62 138 3.30e-13 69.3
MS.gene042663.t1 AT5G18240 42.857 91 46 2 168 253 10 99 3.47e-13 70.5
MS.gene042663.t1 AT5G18240 42.857 91 46 2 168 253 10 99 3.47e-13 70.5
MS.gene042663.t1 AT2G01060 58.182 55 23 0 199 253 15 69 3.84e-13 69.7
MS.gene042663.t1 AT3G10760 46.667 75 36 2 186 257 89 162 7.19e-13 69.3
MS.gene042663.t1 AT2G06020 44.737 76 34 1 186 253 65 140 1.13e-12 68.2
MS.gene042663.t1 AT3G16857 47.945 73 35 2 187 257 153 224 2.35e-12 68.6
MS.gene042663.t1 AT3G16857 47.945 73 35 2 187 257 223 294 2.53e-12 68.6
MS.gene042663.t1 AT3G16857 47.945 73 35 2 187 257 223 294 3.06e-12 68.2
MS.gene042663.t1 AT2G40970 51.667 60 28 1 198 257 104 162 3.24e-12 66.2
MS.gene042663.t1 AT2G20400 59.259 54 22 0 199 252 231 284 4.09e-12 67.4
MS.gene042663.t1 AT2G20400 59.259 54 22 0 199 252 231 284 4.11e-12 67.4
MS.gene042663.t1 AT3G04030 43.678 87 43 2 172 253 14 99 4.67e-12 67.0
MS.gene042663.t1 AT2G01760 31.707 164 96 5 198 361 199 346 5.00e-12 67.0
MS.gene042663.t1 AT3G04030 43.678 87 43 2 172 253 14 99 5.13e-12 67.0
MS.gene042663.t1 AT2G01760 31.707 164 96 5 198 361 227 374 5.71e-12 67.0
MS.gene042663.t1 AT4G13640 58.182 55 23 0 199 253 37 91 6.00e-12 66.2
MS.gene042663.t1 AT4G13640 58.182 55 23 0 199 253 37 91 6.36e-12 65.9
MS.gene042663.t1 AT2G01760 31.707 164 96 5 198 361 134 281 7.04e-12 66.2
MS.gene042663.t1 AT3G24120 58.182 55 23 0 199 253 41 95 7.64e-12 65.9
MS.gene042663.t1 AT4G28610 47.761 67 35 0 186 252 212 278 7.96e-12 66.6
MS.gene042663.t1 AT3G24120 58.182 55 23 0 199 253 41 95 8.06e-12 65.9
MS.gene042663.t1 AT5G59570 47.619 63 32 1 195 257 137 198 9.18e-12 65.5
MS.gene042663.t1 AT5G59570 47.619 63 32 1 195 257 137 198 9.18e-12 65.5
MS.gene042663.t1 AT1G79430 58.182 55 23 0 199 253 34 88 1.35e-11 65.5
MS.gene042663.t1 AT3G46640 42.105 76 43 1 198 273 143 217 1.50e-11 65.1
MS.gene042663.t1 AT3G46640 42.105 76 43 1 198 273 143 217 1.50e-11 65.1
MS.gene042663.t1 AT3G46640 42.105 76 43 1 198 273 143 217 1.50e-11 65.1
MS.gene042663.t1 AT3G12730 56.364 55 24 0 199 253 23 77 1.95e-11 63.9
MS.gene042663.t1 AT4G04605 53.846 52 24 0 201 252 16 67 2.20e-11 63.5
MS.gene042663.t1 AT3G04450 55.556 54 24 0 199 252 238 291 2.39e-11 64.3
MS.gene042663.t1 AT5G29000 45.833 72 39 0 181 252 213 284 2.53e-11 64.3
MS.gene042663.t1 AT4G31920 48.571 70 34 2 189 257 172 240 2.80e-11 65.1
MS.gene042663.t1 AT3G04450 55.556 54 24 0 199 252 198 251 4.27e-11 64.3
MS.gene042663.t1 AT3G04450 55.556 54 24 0 199 252 198 251 4.27e-11 64.3
MS.gene042663.t1 AT3G04450 55.556 54 24 0 199 252 238 291 4.28e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.10e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.13e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.13e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.13e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.13e-11 64.3
MS.gene042663.t1 AT4G18020 41.573 89 47 3 182 268 277 362 5.13e-11 64.3
MS.gene042663.t1 AT5G06800 50.909 55 27 0 199 253 192 246 5.39e-11 63.2
MS.gene042663.t1 AT3G13040 44.872 78 42 1 176 253 219 295 6.94e-11 63.9
MS.gene042663.t1 AT3G13040 44.872 78 42 1 176 253 219 295 6.94e-11 63.9
MS.gene042663.t1 AT3G13040 44.872 78 42 1 176 253 219 295 6.94e-11 63.9
MS.gene042663.t1 AT5G29000 45.833 72 39 0 181 252 213 284 7.57e-11 63.5
MS.gene042663.t1 AT5G29000 55.556 54 24 0 199 252 188 241 9.05e-11 63.2
MS.gene042663.t1 AT5G29000 55.556 54 24 0 199 252 188 241 9.05e-11 63.2

Find 76 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGAATTGTTGTGAATTAT+TGG 0.251586 5.2:-45891305 None:intergenic
TTCTCCTGACACTGTTGTTT+TGG 0.263091 5.2:-45891399 None:intergenic
ACATACAGCTCCTATGTTCT+TGG 0.291304 5.2:+45891331 MS.gene042663:CDS
CAGCAGCGATTTCCTCTTTG+TGG 0.314629 5.2:-45890555 None:intergenic
TTTCAGCATCTTGAAGTTGT+AGG 0.327142 5.2:-45891602 None:intergenic
ATTAGTTCCCGGATCTGTTT+CGG 0.330999 5.2:-45890873 None:intergenic
TTGTTGCCGCTTTCAACAAC+TGG 0.346746 5.2:-45890652 None:intergenic
TAGAATTGTTGTGAATTATT+GGG 0.348297 5.2:-45891304 None:intergenic
TGAAGTCAAAAGTCATTTGC+AGG 0.374794 5.2:+45890920 MS.gene042663:CDS
ACGTGTAAGCAACAGTTGTT+TGG 0.397960 5.2:+45889916 MS.gene042663:CDS
AGAGAAAGAGGAAGGTCTCT+AGG 0.412125 5.2:-45889740 None:intergenic
GCAGCGTCGGTGCTGGTCAC+AGG 0.421709 5.2:+45890699 MS.gene042663:CDS
TATGTCCAACCACAAGAATA+TGG 0.427221 5.2:+45891365 MS.gene042663:CDS
CTGTTGCTTACACGTTTCAA+TGG 0.431651 5.2:-45889908 None:intergenic
TATACCTCTTTAGCAGATGA+AGG 0.432154 5.2:-45890138 None:intergenic
CTGCCACGCCGAAACAGATC+CGG 0.438655 5.2:+45890865 MS.gene042663:CDS
TGGAACACAATCGGAGTGTT+CGG 0.442544 5.2:+45889936 MS.gene042663:CDS
ACCACAGTTACTACTCCAAC+AGG 0.442991 5.2:+45891425 MS.gene042663:CDS
CAACCCTTCATCTGCTAAAG+AGG 0.450187 5.2:+45890134 MS.gene042663:CDS
CATCGACTTTCATTAGTTCC+CGG 0.451625 5.2:-45890884 None:intergenic
AGTTTATTCCTATCAAGAAA+AGG 0.453926 5.2:+45889995 MS.gene042663:CDS
AGCAGGTACTTGAGGCGAAC+TGG 0.464004 5.2:-45890614 None:intergenic
CATGCCCTTCAACAACTTGG+CGG 0.464333 5.2:+45890742 MS.gene042663:CDS
TCTTTAGCAGATGAAGGGTT+GGG 0.465236 5.2:-45890132 None:intergenic
GTTTATTCCTATCAAGAAAA+GGG 0.474072 5.2:+45889996 MS.gene042663:CDS
CACATAAGATGAAATCTGAT+TGG 0.476812 5.2:+45890085 MS.gene042663:CDS
TTGCATGCCCTTCAACAACT+TGG 0.479053 5.2:+45890739 MS.gene042663:CDS
AGAGGAAGCAGCGTCGGTGC+TGG 0.486844 5.2:+45890692 MS.gene042663:CDS
CTCTTTAGCAGATGAAGGGT+TGG 0.487382 5.2:-45890133 None:intergenic
GCCACCAAAACAACAGTGTC+AGG 0.487737 5.2:+45891395 MS.gene042663:CDS
ATAACTGTTGTTGTAGATGA+AGG 0.491980 5.2:-45891473 None:intergenic
TTGAACCGCCAAGTTGTTGA+AGG 0.494195 5.2:-45890747 None:intergenic
GAGAAAGAGGAAGGTCTCTA+GGG 0.495572 5.2:-45889739 None:intergenic
GCGATTTCCTCTTTGTGGAA+TGG 0.495901 5.2:-45890550 None:intergenic
ACAGTTGTTTGGAACACAAT+CGG 0.495980 5.2:+45889927 MS.gene042663:CDS
GTAACAAGCTCAAGAGAAAG+AGG 0.505480 5.2:-45889752 None:intergenic
ACAAGAAAAGAAGAAAATCC+AGG 0.515271 5.2:+45889712 MS.gene042663:CDS
CTGCACCATATTCTTGTGGT+TGG 0.520262 5.2:-45891370 None:intergenic
GAAGGTCTCTAGGGAAAACC+TGG 0.528167 5.2:-45889730 None:intergenic
TGCCACGCCGAAACAGATCC+GGG 0.529744 5.2:+45890866 MS.gene042663:CDS
GGCTAAGATCTGTTCAGTTG+TGG 0.535273 5.2:+45890106 MS.gene042663:CDS
GTTGTAGAAGTGAAGAGAAA+TGG 0.535669 5.2:+45890514 MS.gene042663:CDS
GGCAACAAAAGAGACGATAA+AGG 0.545011 5.2:+45890667 MS.gene042663:CDS
ATTTCAACCATTCCACAAAG+AGG 0.547227 5.2:+45890543 MS.gene042663:CDS
GAACAGACATCAACGGATGA+AGG 0.553205 5.2:+45889958 MS.gene042663:CDS
ACAGCTCCTATGTTCTTGGT+AGG 0.553648 5.2:+45891335 MS.gene042663:CDS
ATACCTCTTTAGCAGATGAA+GGG 0.556739 5.2:-45890137 None:intergenic
TCGTGACTATATCCTAGCAT+TGG 0.561341 5.2:+45889688 MS.gene042663:CDS
TGAACCGCCAAGTTGTTGAA+GGG 0.566682 5.2:-45890746 None:intergenic
GCTACTGCACCATATTCTTG+TGG 0.569246 5.2:-45891374 None:intergenic
TGAGTGGAACATGATGAGGC+AGG 0.570388 5.2:-45891575 None:intergenic
GGTTCAGTAGAGCTTGTAGC+AGG 0.575341 5.2:-45890631 None:intergenic
GAGCTTGTAGCAGGTACTTG+AGG 0.576365 5.2:-45890622 None:intergenic
CAGCTCCTATGTTCTTGGTA+GGG 0.576915 5.2:+45891336 MS.gene042663:CDS
GAGTGGAACATGATGAGGCA+GGG 0.580949 5.2:-45891574 None:intergenic
AATTTCGTCTGCACACAAGA+AGG 0.582559 5.2:+45891279 MS.gene042663:CDS
ACAGGTGCATAAATTCCTGT+TGG 0.585331 5.2:-45891440 None:intergenic
CAAGCTCAAGAGAAAGAGGA+AGG 0.593272 5.2:-45889748 None:intergenic
AGTGGAACATGATGAGGCAG+GGG 0.594079 5.2:-45891573 None:intergenic
ATGTTCCCTACCAAGAACAT+AGG 0.596023 5.2:-45891341 None:intergenic
AACAGGAATTTATGCACCTG+TGG 0.600669 5.2:+45891442 MS.gene042663:CDS
TCTCTTGAGCTTGTTACACA+AGG 0.614804 5.2:+45889758 MS.gene042663:CDS
GTAGAAGTGAAGAGAAATGG+TGG 0.614913 5.2:+45890517 MS.gene042663:CDS
AAGGTCAGAGGAAGCAGCGT+CGG 0.617450 5.2:+45890686 MS.gene042663:CDS
AAAGAGACGATAAAGGTCAG+AGG 0.623540 5.2:+45890674 MS.gene042663:CDS
CGGGAACTAATGAAAGTCGA+TGG 0.628624 5.2:+45890885 MS.gene042663:CDS
GGTACTTGAGGCGAACTGGT+TGG 0.629043 5.2:-45890610 None:intergenic
ACTGAACCAGTTGTTGAAAG+CGG 0.633483 5.2:+45890646 MS.gene042663:CDS
TGTTTGTTCAGGATGTACCT+AGG 0.649194 5.2:+45890482 MS.gene042663:intron
TGAAGTTGTAGGTGTATGAG+TGG 0.650979 5.2:-45891591 None:intergenic
GATGAAGGATGTGTAGCCAC+AGG 0.668784 5.2:-45891458 None:intergenic
GTGTTCGGAACAGACATCAA+CGG 0.672963 5.2:+45889951 MS.gene042663:CDS
CAATTGCGATGAAAATGATG+AGG 0.674430 5.2:+45890029 MS.gene042663:CDS
TCCTGTTGGAGTAGTAACTG+TGG 0.676951 5.2:-45891426 None:intergenic
TTCCCGGATCTGTTTCGGCG+TGG 0.690024 5.2:-45890868 None:intergenic
TGTATGAGTGGAACATGATG+AGG 0.702899 5.2:-45891579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATTTATTAAAATCAACTT+TGG - chr5.2:45890278-45890297 None:intergenic 15.0%
!! TTAATCATAGAAAATTACAT+TGG + chr5.2:45890197-45890216 MS.gene042663:intron 15.0%
!!! GTCTAACTTTTTTTAATTTT+CGG + chr5.2:45891245-45891264 MS.gene042663:intron 15.0%
!!! TCAAATTTTTTCTTAAACTT+TGG - chr5.2:45891503-45891522 None:intergenic 15.0%
!! TAGAATTGTTGTGAATTATT+GGG - chr5.2:45891307-45891326 None:intergenic 20.0%
!! TTAGAATTGTTGTGAATTAT+TGG - chr5.2:45891308-45891327 None:intergenic 20.0%
!!! GTGTATATTTTTTTTTCTTC+TGG - chr5.2:45889796-45889815 None:intergenic 20.0%
! AAAAAATCAAAACGATGAAG+TGG - chr5.2:45890954-45890973 None:intergenic 25.0%
! ACACAATTCACATTTAGATT+CGG - chr5.2:45890831-45890850 None:intergenic 25.0%
! AGTTTATTCCTATCAAGAAA+AGG + chr5.2:45889995-45890014 MS.gene042663:CDS 25.0%
! CAAGTTAATGTTTCTTTCAT+TGG + chr5.2:45889828-45889847 MS.gene042663:intron 25.0%
! CAATGAAAGAAACATTAACT+TGG - chr5.2:45889830-45889849 None:intergenic 25.0%
! GACAAGGAAAATTATACAAA+TGG - chr5.2:45890980-45890999 None:intergenic 25.0%
! GATATACTTTAAGCTTGATT+TGG - chr5.2:45891098-45891117 None:intergenic 25.0%
! GTTTATTCCTATCAAGAAAA+GGG + chr5.2:45889996-45890015 MS.gene042663:CDS 25.0%
! TCTTCTTGATCGTTAAATTA+TGG + chr5.2:45891223-45891242 MS.gene042663:intron 25.0%
!!! ACTTTTGATTAGGACTTTTT+CGG + chr5.2:45890349-45890368 MS.gene042663:intron 25.0%
!!! CTTTTTTTAATTTTCGGACA+TGG + chr5.2:45891251-45891270 MS.gene042663:intron 25.0%
ACAAGAAAAGAAGAAAATCC+AGG + chr5.2:45889712-45889731 MS.gene042663:CDS 30.0%
AGAATGTGAAATGTTTGTTC+AGG + chr5.2:45890471-45890490 MS.gene042663:intron 30.0%
ATAACTGTTGTTGTAGATGA+AGG - chr5.2:45891476-45891495 None:intergenic 30.0%
CACATAAGATGAAATCTGAT+TGG + chr5.2:45890085-45890104 MS.gene042663:CDS 30.0%
CGTACAAAAAAATTCGACTT+TGG - chr5.2:45890175-45890194 None:intergenic 30.0%
CTAACTCTAATCTTCATCTA+CGG - chr5.2:45891054-45891073 None:intergenic 30.0%
CTGTCTAACCTTTAATCTAA+CGG + chr5.2:45891129-45891148 MS.gene042663:intron 30.0%
GAAAATTACATTGGACTGAA+AGG + chr5.2:45890206-45890225 MS.gene042663:intron 30.0%
TTACTAACCACGTATAATCT+TGG - chr5.2:45891008-45891027 None:intergenic 30.0%
! TCATCATTTTCATCGCAATT+GGG - chr5.2:45890030-45890049 None:intergenic 30.0%
!! GAACTGTGAAACTTTTGATT+AGG + chr5.2:45890339-45890358 MS.gene042663:intron 30.0%
!! TCTACAACATTTGTTTTCCT+AGG - chr5.2:45890502-45890521 None:intergenic 30.0%
!! TGTTTCTTTCATTGGTTTCT+TGG + chr5.2:45889836-45889855 MS.gene042663:intron 30.0%
ATACCTCTTTAGCAGATGAA+GGG - chr5.2:45890140-45890159 None:intergenic 35.0%
ATTTCAACCATTCCACAAAG+AGG + chr5.2:45890543-45890562 MS.gene042663:CDS 35.0%
CAATGTATCAAGCAGACAAT+AGG - chr5.2:45890394-45890413 None:intergenic 35.0%
CAATTGCGATGAAAATGATG+AGG + chr5.2:45890029-45890048 MS.gene042663:CDS 35.0%
GATCTTGACCGTTAGATTAA+AGG - chr5.2:45891140-45891159 None:intergenic 35.0%
GTTGTAGAAGTGAAGAGAAA+TGG + chr5.2:45890514-45890533 MS.gene042663:CDS 35.0%
TATACCTCTTTAGCAGATGA+AGG - chr5.2:45890141-45890160 None:intergenic 35.0%
TATGTCCAACCACAAGAATA+TGG + chr5.2:45891365-45891384 MS.gene042663:CDS 35.0%
TGAAGTCAAAAGTCATTTGC+AGG + chr5.2:45890920-45890939 MS.gene042663:CDS 35.0%
TGTCTGCTTGATACATTGAA+TGG + chr5.2:45890396-45890415 MS.gene042663:intron 35.0%
! ACAGTTGTTTGGAACACAAT+CGG + chr5.2:45889927-45889946 MS.gene042663:CDS 35.0%
! ATCATTTTCATCGCAATTGG+GGG - chr5.2:45890028-45890047 None:intergenic 35.0%
! CATCATTTTCATCGCAATTG+GGG - chr5.2:45890029-45890048 None:intergenic 35.0%
! CTCATCATTTTCATCGCAAT+TGG - chr5.2:45890031-45890050 None:intergenic 35.0%
! TATAATCTTGGTCTGAGACA+AGG - chr5.2:45890996-45891015 None:intergenic 35.0%
! TCTTTTCTTGTTCCAATGCT+AGG - chr5.2:45889703-45889722 None:intergenic 35.0%
!!! ATGACTTTTGACTTCATCGT+TGG - chr5.2:45890916-45890935 None:intergenic 35.0%
AAAGAGACGATAAAGGTCAG+AGG + chr5.2:45890674-45890693 MS.gene042663:CDS 40.0%
AACAGGAATTTATGCACCTG+TGG + chr5.2:45891442-45891461 MS.gene042663:CDS 40.0%
AATTTCGTCTGCACACAAGA+AGG + chr5.2:45891279-45891298 MS.gene042663:CDS 40.0%
ACAGGTGCATAAATTCCTGT+TGG - chr5.2:45891443-45891462 None:intergenic 40.0%
ACATACAGCTCCTATGTTCT+TGG + chr5.2:45891331-45891350 MS.gene042663:CDS 40.0%
ACGTGTAAGCAACAGTTGTT+TGG + chr5.2:45889916-45889935 MS.gene042663:CDS 40.0%
ACTGAACCAGTTGTTGAAAG+CGG + chr5.2:45890646-45890665 MS.gene042663:CDS 40.0%
AGTCGCAATTCTGATAGTCA+AGG - chr5.2:45891162-45891181 None:intergenic 40.0%
ATGTTCCCTACCAAGAACAT+AGG - chr5.2:45891344-45891363 None:intergenic 40.0%
ATTAGTTCCCGGATCTGTTT+CGG - chr5.2:45890876-45890895 None:intergenic 40.0%
GGCAACAAAAGAGACGATAA+AGG + chr5.2:45890667-45890686 MS.gene042663:CDS 40.0%
GTAACAAGCTCAAGAGAAAG+AGG - chr5.2:45889755-45889774 None:intergenic 40.0%
GTAGAAGTGAAGAGAAATGG+TGG + chr5.2:45890517-45890536 MS.gene042663:CDS 40.0%
TCATCTACGGTAAAAATCCG+TGG - chr5.2:45891041-45891060 None:intergenic 40.0%
TCGTGACTATATCCTAGCAT+TGG + chr5.2:45889688-45889707 MS.gene042663:CDS 40.0%
TCTCAGACCAAGATTATACG+TGG + chr5.2:45890998-45891017 MS.gene042663:intron 40.0%
TCTTTAGCAGATGAAGGGTT+GGG - chr5.2:45890135-45890154 None:intergenic 40.0%
TGAAGTTGTAGGTGTATGAG+TGG - chr5.2:45891594-45891613 None:intergenic 40.0%
TGTATGAGTGGAACATGATG+AGG - chr5.2:45891582-45891601 None:intergenic 40.0%
TGTTTGTTCAGGATGTACCT+AGG + chr5.2:45890482-45890501 MS.gene042663:intron 40.0%
TTAGTAAACCCTGCAATCCA+CGG + chr5.2:45891021-45891040 MS.gene042663:intron 40.0%
! CTGTTGCTTACACGTTTCAA+TGG - chr5.2:45889911-45889930 None:intergenic 40.0%
! GACAATGCCCTTTTCTTGAT+AGG - chr5.2:45890006-45890025 None:intergenic 40.0%
! GTAAAAATCCGTGGATTGCA+GGG - chr5.2:45891032-45891051 None:intergenic 40.0%
!! CATCGACTTTCATTAGTTCC+CGG - chr5.2:45890887-45890906 None:intergenic 40.0%
!! TCTCTTGAGCTTGTTACACA+AGG + chr5.2:45889758-45889777 MS.gene042663:CDS 40.0%
!! TTCTCCTGACACTGTTGTTT+TGG - chr5.2:45891402-45891421 None:intergenic 40.0%
!!! GGATGAAGGTCTTGTTTTTG+AGG + chr5.2:45889972-45889991 MS.gene042663:CDS 40.0%
!!! TTTTGACTTCATCGTTGGTG+AGG - chr5.2:45890911-45890930 None:intergenic 40.0%
ACAGCTCCTATGTTCTTGGT+AGG + chr5.2:45891335-45891354 MS.gene042663:CDS 45.0%
ACCACAGTTACTACTCCAAC+AGG + chr5.2:45891425-45891444 MS.gene042663:CDS 45.0%
AGAGAAAGAGGAAGGTCTCT+AGG - chr5.2:45889743-45889762 None:intergenic 45.0%
CAACCCTTCATCTGCTAAAG+AGG + chr5.2:45890134-45890153 MS.gene042663:CDS 45.0%
CAAGCTCAAGAGAAAGAGGA+AGG - chr5.2:45889751-45889770 None:intergenic 45.0%
CAGCTCCTATGTTCTTGGTA+GGG + chr5.2:45891336-45891355 MS.gene042663:CDS 45.0%
CGGGAACTAATGAAAGTCGA+TGG + chr5.2:45890885-45890904 MS.gene042663:CDS 45.0%
CTCTTTAGCAGATGAAGGGT+TGG - chr5.2:45890136-45890155 None:intergenic 45.0%
CTGCACCATATTCTTGTGGT+TGG - chr5.2:45891373-45891392 None:intergenic 45.0%
GAACAGACATCAACGGATGA+AGG + chr5.2:45889958-45889977 MS.gene042663:CDS 45.0%
GAGAAAGAGGAAGGTCTCTA+GGG - chr5.2:45889742-45889761 None:intergenic 45.0%
GCGATTTCCTCTTTGTGGAA+TGG - chr5.2:45890553-45890572 None:intergenic 45.0%
GCTACTGCACCATATTCTTG+TGG - chr5.2:45891377-45891396 None:intergenic 45.0%
GGCTAAGATCTGTTCAGTTG+TGG + chr5.2:45890106-45890125 MS.gene042663:CDS 45.0%
GTGTTCGGAACAGACATCAA+CGG + chr5.2:45889951-45889970 MS.gene042663:CDS 45.0%
TCCTGTTGGAGTAGTAACTG+TGG - chr5.2:45891429-45891448 None:intergenic 45.0%
TGAACCGCCAAGTTGTTGAA+GGG - chr5.2:45890749-45890768 None:intergenic 45.0%
TTGAACCGCCAAGTTGTTGA+AGG - chr5.2:45890750-45890769 None:intergenic 45.0%
TTGCATGCCCTTCAACAACT+TGG + chr5.2:45890739-45890758 MS.gene042663:CDS 45.0%
! GGTAAAAATCCGTGGATTGC+AGG - chr5.2:45891033-45891052 None:intergenic 45.0%
! TGGAACACAATCGGAGTGTT+CGG + chr5.2:45889936-45889955 MS.gene042663:CDS 45.0%
!! TTGTTGCCGCTTTCAACAAC+TGG - chr5.2:45890655-45890674 None:intergenic 45.0%
!!! TCCTGACACTGTTGTTTTGG+TGG - chr5.2:45891399-45891418 None:intergenic 45.0%
AGTGGAACATGATGAGGCAG+GGG - chr5.2:45891576-45891595 None:intergenic 50.0%
CAGCAGCGATTTCCTCTTTG+TGG - chr5.2:45890558-45890577 None:intergenic 50.0%
CATGCCCTTCAACAACTTGG+CGG + chr5.2:45890742-45890761 MS.gene042663:CDS 50.0%
GAGCTTGTAGCAGGTACTTG+AGG - chr5.2:45890625-45890644 None:intergenic 50.0%
GAGTGGAACATGATGAGGCA+GGG - chr5.2:45891577-45891596 None:intergenic 50.0%
GATGAAGGATGTGTAGCCAC+AGG - chr5.2:45891461-45891480 None:intergenic 50.0%
GCCACCAAAACAACAGTGTC+AGG + chr5.2:45891395-45891414 MS.gene042663:CDS 50.0%
GGTTCAGTAGAGCTTGTAGC+AGG - chr5.2:45890634-45890653 None:intergenic 50.0%
TGAGTGGAACATGATGAGGC+AGG - chr5.2:45891578-45891597 None:intergenic 50.0%
TTGGTTTCGCCATCCAGAGA+CGG - chr5.2:45890259-45890278 None:intergenic 50.0%
! GAAGGTCTCTAGGGAAAACC+TGG - chr5.2:45889733-45889752 None:intergenic 50.0%
AAGGTCAGAGGAAGCAGCGT+CGG + chr5.2:45890686-45890705 MS.gene042663:CDS 55.0%
AGCAGGTACTTGAGGCGAAC+TGG - chr5.2:45890617-45890636 None:intergenic 55.0%
GGTACTTGAGGCGAACTGGT+TGG - chr5.2:45890613-45890632 None:intergenic 55.0%
CGCCATCCAGAGACGGTTAG+CGG - chr5.2:45890252-45890271 None:intergenic 60.0%
CTGCCACGCCGAAACAGATC+CGG + chr5.2:45890865-45890884 MS.gene042663:CDS 60.0%
TGCCACGCCGAAACAGATCC+GGG + chr5.2:45890866-45890885 MS.gene042663:CDS 60.0%
TGCCGCTAACCGTCTCTGGA+TGG + chr5.2:45890247-45890266 MS.gene042663:intron 60.0%
TTCCCGGATCTGTTTCGGCG+TGG - chr5.2:45890871-45890890 None:intergenic 60.0%
! GTTGTGCCGCTAACCGTCTC+TGG + chr5.2:45890243-45890262 MS.gene042663:intron 60.0%
!! AGAGGAAGCAGCGTCGGTGC+TGG + chr5.2:45890692-45890711 MS.gene042663:CDS 65.0%
!! GCAGCGTCGGTGCTGGTCAC+AGG + chr5.2:45890699-45890718 MS.gene042663:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 45889638 45891622 45889638 ID=MS.gene042663
chr5.2 mRNA 45889638 45891622 45889638 ID=MS.gene042663.t1;Parent=MS.gene042663
chr5.2 exon 45889638 45889779 45889638 ID=MS.gene042663.t1.exon1;Parent=MS.gene042663.t1
chr5.2 CDS 45889638 45889779 45889638 ID=cds.MS.gene042663.t1;Parent=MS.gene042663.t1
chr5.2 exon 45889908 45890155 45889908 ID=MS.gene042663.t1.exon2;Parent=MS.gene042663.t1
chr5.2 CDS 45889908 45890155 45889908 ID=cds.MS.gene042663.t1;Parent=MS.gene042663.t1
chr5.2 exon 45890493 45890772 45890493 ID=MS.gene042663.t1.exon3;Parent=MS.gene042663.t1
chr5.2 CDS 45890493 45890772 45890493 ID=cds.MS.gene042663.t1;Parent=MS.gene042663.t1
chr5.2 exon 45890865 45890941 45890865 ID=MS.gene042663.t1.exon4;Parent=MS.gene042663.t1
chr5.2 CDS 45890865 45890941 45890865 ID=cds.MS.gene042663.t1;Parent=MS.gene042663.t1
chr5.2 exon 45891278 45891622 45891278 ID=MS.gene042663.t1.exon5;Parent=MS.gene042663.t1
chr5.2 CDS 45891278 45891622 45891278 ID=cds.MS.gene042663.t1;Parent=MS.gene042663.t1
Gene Sequence

>MS.gene042663

ATGGTTTCAATGCAATTCGATGATAAAATGCACTCGCATAAAATGAAATTTCGTGACTATATCCTAGCATTGGAACAAGAAAAGAAGAAAATCCAGGTTTTCCCTAGAGACCTTCCTCTTTCTCTTGAGCTTGTTACACAAGGTAAACAAACAAACCAGAAGAAAAAAAAATATACACTTTGTTTCATACCAAGTTAATGTTTCTTTCATTGGTTTCTTGGTCTGTGTTTTTCGACTTTTTTTTATATGTTATGTTTTGTGAATATGCAGCCATTGAAACGTGTAAGCAACAGTTGTTTGGAACACAATCGGAGTGTTCGGAACAGACATCAACGGATGAAGGTCTTGTTTTTGAGGAGTTTATTCCTATCAAGAAAAGGGCATTGTCCCCCAATTGCGATGAAAATGATGAGGAAGATGATGATGATAATGATGAGCAACATTCATCACATAAGATGAAATCTGATTGGCTAAGATCTGTTCAGTTGTGGAATCCCAACCCTTCATCTGCTAAAGAGGTATAAAATTGCAAAACCAAAGTCGAATTTTTTTGTACGATTTAATCATAGAAAATTACATTGGACTGAAAGGCAAGCAGACAACAAGTTGTGCCGCTAACCGTCTCTGGATGGCGAAACCAAAGTTGATTTTAATAAATGAATTTTTATTTTATACGCAAACTTTAGTAAATTAGCGATTTTGAACTGTGAAACTTTTGATTAGGACTTTTTCGGTGTCACTCAATTCATCAAGCCTATTGTCTGCTTGATACATTGAATGGAATTGTTGCTTTAGAACTCTTTGTTTGTTTCATAATTGAATAGAAATTTAAGAGAATGTGAAATGTTTGTTCAGGATGTACCTAGGAAAACAAATGTTGTAGAAGTGAAGAGAAATGGTGGAGCATTTCAACCATTCCACAAAGAGGAAATCGCTGCTGAAAAAGATAATGCATTAGAAAGTGATAAAGCACCAACCAGTTCGCCTCAAGTACCTGCTACAAGCTCTACTGAACCAGTTGTTGAAAGCGGCAACAAAAGAGACGATAAAGGTCAGAGGAAGCAGCGTCGGTGCTGGTCACAGGAATTGCACAAACGCTTCTTGCATGCCCTTCAACAACTTGGCGGTTCAAATTGTGAGTGAATATATTACTCATCAGCATAATTAGTTCATTCTTATTGTTAATTGTTCCGAATCTAAATGTGAATTGTGTGTGACATGTTGTAGCTGCCACGCCGAAACAGATCCGGGAACTAATGAAAGTCGATGGCCTCACCAACGATGAAGTCAAAAGTCATTTGCAGGTTAGTTTACCACTTCATCGTTTTGATTTTTTGTTCCATTTGTATAATTTTCCTTGTCTCAGACCAAGATTATACGTGGTTAGTAAACCCTGCAATCCACGGATTTTTACCGTAGATGAAGATTAGAGTTAGACATCTCGTATTCTGATTATACCAAATCAAGCTTAAAGTATATCTCGAATACGAGCTGTCTAACCTTTAATCTAACGGTCAAGATCCTTGACTATCAGAATTGCGACTGCATAGAACTCGAATTCATATTAGCAGATATCACTATACGATCTTCTTGATCGTTAAATTATGGTCTAACTTTTTTTAATTTTCGGACATGGACAGAAATTTCGTCTGCACACAAGAAGGAGCCCAATAATTCACAACAATTCTAATTCACATACAGCTCCTATGTTCTTGGTAGGGAACATTTATGTCCAACCACAAGAATATGGTGCAGTAGCCACCAAAACAACAGTGTCAGGAGAATTGACCACAGTTACTACTCCAACAGGAATTTATGCACCTGTGGCTACACATCCTTCATCTACAACAACAGTTATAAAACCAAAGTTTAAGAAAAAATTTGAGTTGAGTGAGAATTCACATTCTGTAGAAAGAATTGTTGCTCATTCTAACTCCCCTGCCTCATCATGTTCCACTCATACACCTACAACTTCAAGATGCTGA
Protein sequence

>MS.gene042663.t1

MVSMQFDDKMHSHKMKFRDYILALEQEKKKIQVFPRDLPLSLELVTQAIETCKQQLFGTQSECSEQTSTDEGLVFEEFIPIKKRALSPNCDENDEEDDDDNDEQHSSHKMKSDWLRSVQLWNPNPSSAKEDVPRKTNVVEVKRNGGAFQPFHKEEIAAEKDNALESDKAPTSSPQVPATSSTEPVVESGNKRDDKGQRKQRRCWSQELHKRFLHALQQLGGSNSATPKQIRELMKVDGLTNDEVKSHLQKFRLHTRRSPIIHNNSNSHTAPMFLVGNIYVQPQEYGAVATKTTVSGELTTVTTPTGIYAPVATHPSSTTTVIKPKFKKKFELSENSHSVERIVAHSNSPASSCSTHTPTTSRC