AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043074

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043074.t1 MTR_4g031910 71.795 234 49 2 1 222 1 229 1.97e-111 319
MS.gene043074.t1 MTR_4g032290 69.262 244 40 2 1 222 1 231 1.12e-106 307
MS.gene043074.t1 MTR_4g032620 57.576 231 89 1 1 222 1 231 2.66e-96 281
MS.gene043074.t1 MTR_8g036130 58.300 247 78 4 1 222 1 247 6.86e-92 270
MS.gene043074.t1 MTR_4g032260 79.618 157 32 0 1 157 1 157 2.65e-80 238
MS.gene043074.t1 MTR_3g065100 48.548 241 99 4 1 219 1 238 1.89e-65 202
MS.gene043074.t1 MTR_3g466980 46.330 218 103 3 1 214 1 208 4.18e-63 196
MS.gene043074.t1 MTR_2g035610 72.951 122 33 0 1 122 1 122 1.85e-52 166
MS.gene043074.t1 MTR_4g032270 76.042 96 15 1 135 222 36 131 5.22e-45 147
MS.gene043074.t1 MTR_3g466830 48.649 148 75 1 3 150 2 148 1.38e-44 147
MS.gene043074.t1 MTR_4g063790 44.156 154 86 0 1 154 1 154 9.09e-44 145
MS.gene043074.t1 MTR_5g075380 46.358 151 80 1 1 151 1 150 1.06e-43 144
MS.gene043074.t1 MTR_3g467080 41.667 156 91 0 1 156 1 156 1.00e-41 139
MS.gene043074.t1 MTR_5g047580 44.231 156 85 2 1 156 1 154 1.77e-40 136
MS.gene043074.t1 MTR_3g031100 33.929 224 136 4 1 219 1 217 6.12e-40 137
MS.gene043074.t1 MTR_3g466890 45.270 148 80 1 3 150 2 148 9.36e-40 134
MS.gene043074.t1 MTR_2g016210 38.418 177 108 1 1 176 1 177 8.36e-39 134
MS.gene043074.t1 MTR_1g077390 41.026 156 90 2 1 156 1 154 1.74e-37 129
MS.gene043074.t1 MTR_7g011950 42.949 156 87 2 1 156 1 154 3.53e-35 122
MS.gene043074.t1 MTR_1g090783 40.816 147 85 2 3 149 2 146 1.23e-34 121
MS.gene043074.t1 MTR_1g077320 42.949 156 87 2 1 156 1 154 3.62e-34 120
MS.gene043074.t1 MTR_3g465410 45.082 122 67 0 29 150 1 122 9.37e-34 119
MS.gene043074.t1 MTR_1g090710 41.176 153 78 3 1 153 1 141 1.76e-33 118
MS.gene043074.t1 MTR_4g028720 42.308 156 88 2 1 156 1 154 1.98e-33 118
MS.gene043074.t1 MTR_1g090697 40.000 150 88 2 1 150 1 148 2.49e-33 118
MS.gene043074.t1 MTR_1g077300 42.949 156 85 2 1 156 1 152 1.54e-32 116
MS.gene043074.t1 MTR_4g028800 77.143 70 14 1 1 70 1 68 1.67e-32 113
MS.gene043074.t1 MTR_1g084950 42.949 156 87 2 1 156 1 154 3.29e-32 115
MS.gene043074.t1 MTR_7g055800 52.137 117 28 2 1 117 1 89 1.48e-30 108
MS.gene043074.t1 MTR_3g031240 34.810 158 99 2 1 156 1 156 5.19e-30 110
MS.gene043074.t1 MTR_3g466900 39.837 123 73 1 32 154 2 123 3.85e-28 103
MS.gene043074.t1 MTR_7g055790 54.054 111 33 1 1 111 59 151 2.07e-27 102
MS.gene043074.t1 MTR_3g466930 38.655 119 72 1 32 150 2 119 1.04e-25 97.8
MS.gene043074.t1 MTR_7g055940 50.943 106 24 2 1 106 1 78 3.48e-25 94.7
MS.gene043074.t1 MTR_4g019670 40.000 125 75 0 3 127 6 130 1.48e-24 101
MS.gene043074.t1 MTR_7g106510 38.583 127 78 0 3 129 6 132 1.65e-24 101
MS.gene043074.t1 MTR_2g035580 35.669 157 100 1 1 157 1 156 2.18e-24 95.1
MS.gene043074.t1 MTR_3g093900 31.658 199 131 4 3 196 6 204 4.49e-22 94.4
MS.gene043074.t1 MTR_5g047560 34.615 156 84 3 1 156 1 138 5.09e-22 88.2
MS.gene043074.t1 MTR_2g035590 60.494 81 32 0 74 154 1 81 6.82e-21 84.3
MS.gene043074.t1 MTR_4g084780 39.640 111 51 2 74 168 1 111 5.45e-14 67.4
MS.gene043074.t1 MTR_6g018920 32.743 113 69 4 1 107 1 112 1.72e-13 68.9
MS.gene043074.t1 MTR_6g005450 35.398 113 66 4 1 107 1 112 2.31e-13 68.6
MS.gene043074.t1 MTR_1g114730 31.783 129 76 4 1 123 1 123 2.33e-13 68.6
MS.gene043074.t1 MTR_6g005440 36.283 113 65 4 1 107 1 112 3.07e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043074.t1 AT5G48670 53.896 154 70 1 1 154 1 153 3.67e-46 156
MS.gene043074.t1 AT5G26630 42.927 205 106 2 1 195 1 204 3.20e-42 142
MS.gene043074.t1 AT1G65300 44.737 152 83 1 3 154 2 152 6.06e-41 141
MS.gene043074.t1 AT1G65330 45.395 152 82 1 3 154 2 152 1.41e-39 137
MS.gene043074.t1 AT5G26650 45.714 140 75 1 5 144 3 141 5.40e-37 133
MS.gene043074.t1 AT5G27960 46.667 135 71 1 5 139 3 136 1.03e-36 131
MS.gene043074.t1 AT1G31630 39.865 148 87 2 3 150 2 147 1.32e-35 129
MS.gene043074.t1 AT2G28700 38.636 176 101 3 1 174 1 171 7.56e-34 124
MS.gene043074.t1 AT3G05860 37.267 161 100 1 1 161 1 160 1.00e-32 118
MS.gene043074.t1 AT5G27810 55.914 93 41 0 29 121 1 93 2.32e-32 114
MS.gene043074.t1 AT1G31640 39.189 148 88 2 3 150 2 147 3.94e-32 122
MS.gene043074.t1 AT3G05860 37.267 161 100 1 1 161 1 160 5.20e-32 117
MS.gene043074.t1 AT3G05860 37.267 161 100 1 1 161 1 160 7.22e-32 117
MS.gene043074.t1 AT5G26580 36.806 144 76 2 1 144 1 129 1.97e-25 102
MS.gene043074.t1 AT1G22590 30.247 162 109 1 1 162 1 158 6.70e-24 94.0
MS.gene043074.t1 AT5G06500 36.496 137 80 1 1 137 1 130 1.29e-21 90.1
MS.gene043074.t1 AT2G40210 40.157 127 75 1 1 127 1 126 2.33e-19 85.9
MS.gene043074.t1 AT5G58890 41.593 113 55 5 6 109 6 116 3.00e-16 76.3
MS.gene043074.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.67e-13 68.6
MS.gene043074.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.67e-13 68.6
MS.gene043074.t1 AT2G15660 27.027 148 94 3 38 176 31 173 7.16e-12 63.5
MS.gene043074.t1 AT5G27090 28.221 163 95 6 7 164 23 168 9.10e-11 59.3
MS.gene043074.t1 AT5G27944 32.727 110 62 3 24 131 41 140 9.33e-11 60.1

Find 58 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTGGTGAGATCAATTC+TGG 0.238779 4.2:-73174398 MS.gene043074:CDS
GAGCGAAGAAATGGCAATTA+TGG 0.251384 4.2:-73174519 MS.gene043074:CDS
TACCAATGTCCTTCAAATTA+TGG 0.252012 4.2:+73174588 None:intergenic
TCCTTCAAAGGTAGTAAATC+AGG 0.298213 4.2:+73174368 None:intergenic
AAGAAAGGGTCTATTGAAAA+AGG 0.312767 4.2:-73174948 MS.gene043074:CDS
CCCTGATTTACTACCTTTGA+AGG 0.324619 4.2:-73174369 MS.gene043074:CDS
ATGAGCCTCAACCAGAGATT+TGG 0.345216 4.2:-73174868 MS.gene043074:CDS
ATCTCTGGTTGAGGCTCATA+AGG 0.350685 4.2:+73174872 None:intergenic
AAAATGTCATCACCATTAAT+AGG 0.355958 4.2:+73174476 None:intergenic
TTTGCAAAGTCAATTATTGA+TGG 0.379269 4.2:-73174456 MS.gene043074:CDS
GAAGGACATTGGTAGACATT+TGG 0.385901 4.2:-73174579 MS.gene043074:CDS
AGCGAAGAAATGGCAATTAT+GGG 0.425600 4.2:-73174518 MS.gene043074:CDS
AGCATCTAGGAGCGAAGAAA+TGG 0.428793 4.2:-73174528 MS.gene043074:CDS
TCTGGTGAAGGCCAAATCTC+TGG 0.429918 4.2:+73174857 None:intergenic
GTTGATTGACCATAATTTGA+AGG 0.445081 4.2:-73174597 MS.gene043074:CDS
TGTGCACAACAATATTTCAA+TGG 0.460764 4.2:-73174642 MS.gene043074:CDS
CACAACCATGGCTAGAAGTA+AGG 0.461347 4.2:-73175026 None:intergenic
CTCATAAGGGCCATACACTA+TGG 0.468858 4.2:+73174886 None:intergenic
ATCATACAAGAAAAGAAGAA+AGG 0.473094 4.2:-73174963 MS.gene043074:CDS
CTTGACACAAAGGGTTCTGA+AGG 0.478789 4.2:-73174753 MS.gene043074:CDS
TCAATTATTGATGGACTCAA+TGG 0.482426 4.2:-73174447 MS.gene043074:CDS
CATGTTGGGATACCTATTAA+TGG 0.485702 4.2:-73174488 MS.gene043074:CDS
TCTTCGCTCCTAGATGCTGC+TGG 0.487238 4.2:+73174533 None:intergenic
TTGATTTGAGTTATCTTACT+TGG 0.491177 4.2:-73174619 MS.gene043074:CDS
GATGCTGCTGGAGGTGATAG+TGG 0.492236 4.2:+73174545 None:intergenic
TATCACCTCCAGCAGCATCT+AGG 0.496968 4.2:-73174541 MS.gene043074:CDS
TTATGGGTCATGGACATGTT+GGG 0.500968 4.2:-73174502 MS.gene043074:CDS
TGCAGCATCATTGACAATGA+AGG 0.502295 4.2:+73174994 None:intergenic
ATTATGGGTCATGGACATGT+TGG 0.516074 4.2:-73174503 MS.gene043074:CDS
AGAGACTTTCTTGACACAAA+GGG 0.517022 4.2:-73174762 MS.gene043074:CDS
GACCATAATTTGAAGGACAT+TGG 0.517963 4.2:-73174590 MS.gene043074:CDS
GAAATGGCAATTATGGGTCA+TGG 0.521926 4.2:-73174512 MS.gene043074:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.525855 4.2:+73174765 None:intergenic
TCATACAAGAAAAGAAGAAA+GGG 0.526600 4.2:-73174962 MS.gene043074:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.533701 4.2:+73175021 None:intergenic
GGACATTGGTAGACATTTGG+CGG 0.537707 4.2:-73174576 MS.gene043074:CDS
ATGTATCATTGTCTCAATGA+AGG 0.546520 4.2:-73174671 MS.gene043074:CDS
TCTCTGGTTGAGGCTCATAA+GGG 0.546756 4.2:+73174873 None:intergenic
CCAGAATTGATCTCACCAAA+GGG 0.554343 4.2:+73174398 None:intergenic
GTGCAAGAGACAGTGCCCTT+TGG 0.557727 4.2:-73174413 MS.gene043074:CDS
GAAGCCTGTGCCATAGTGTA+TGG 0.559714 4.2:-73174896 MS.gene043074:CDS
CCTTCAAAGGTAGTAAATCA+GGG 0.575295 4.2:+73174369 None:intergenic
GAGATTTGGCCTTCACCAGA+GGG 0.575380 4.2:-73174854 MS.gene043074:CDS
GAAGGCCAAATCTCTGGTTG+AGG 0.576852 4.2:+73174863 None:intergenic
TCCAGAATTGATCTCACCAA+AGG 0.590517 4.2:+73174397 None:intergenic
ATTGAGAAAAGATCCTTCAA+AGG 0.601093 4.2:+73174356 None:intergenic
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4.2:+73174910 None:intergenic
GATTTGGCCTTCACCAGAGG+GGG 0.601928 4.2:-73174852 MS.gene043074:CDS
TTGATGGACTCAATGGTGGC+TGG 0.607204 4.2:-73174440 MS.gene043074:CDS
AAGAGACTTTCTTGACACAA+AGG 0.607804 4.2:-73174763 MS.gene043074:CDS
AGAGATTTGGCCTTCACCAG+AGG 0.616752 4.2:-73174855 MS.gene043074:CDS
AGGGCCATACACTATGGCAC+AGG 0.641363 4.2:+73174892 None:intergenic
TCATTGTCAATGATGCTGCA+AGG 0.652548 4.2:-73174991 MS.gene043074:CDS
ATTATTGATGGACTCAATGG+TGG 0.666274 4.2:-73174444 MS.gene043074:CDS
TCGCTCCTAGATGCTGCTGG+AGG 0.676090 4.2:+73174536 None:intergenic
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4.2:-73174923 MS.gene043074:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4.2:+73174909 None:intergenic
AGATTTGGCCTTCACCAGAG+GGG 0.703414 4.2:-73174853 MS.gene043074:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATGTCATCACCATTAAT+AGG + chr4.2:73174898-73174917 None:intergenic 25.0%
! ATCATACAAGAAAAGAAGAA+AGG - chr4.2:73174408-73174427 MS.gene043074:CDS 25.0%
! TCATACAAGAAAAGAAGAAA+GGG - chr4.2:73174409-73174428 MS.gene043074:CDS 25.0%
! TTTGCAAAGTCAATTATTGA+TGG - chr4.2:73174915-73174934 MS.gene043074:CDS 25.0%
!! TTGATTTGAGTTATCTTACT+TGG - chr4.2:73174752-73174771 MS.gene043074:CDS 25.0%
AAGAAAGGGTCTATTGAAAA+AGG - chr4.2:73174423-73174442 MS.gene043074:CDS 30.0%
ATGTATCATTGTCTCAATGA+AGG - chr4.2:73174700-73174719 MS.gene043074:CDS 30.0%
ATTGAGAAAAGATCCTTCAA+AGG + chr4.2:73175018-73175037 None:intergenic 30.0%
GTTGATTGACCATAATTTGA+AGG - chr4.2:73174774-73174793 MS.gene043074:CDS 30.0%
TACCAATGTCCTTCAAATTA+TGG + chr4.2:73174786-73174805 None:intergenic 30.0%
TGTGCACAACAATATTTCAA+TGG - chr4.2:73174729-73174748 MS.gene043074:CDS 30.0%
!! TCAATTATTGATGGACTCAA+TGG - chr4.2:73174924-73174943 MS.gene043074:CDS 30.0%
AAGAGACTTTCTTGACACAA+AGG - chr4.2:73174608-73174627 MS.gene043074:CDS 35.0%
AGAGACTTTCTTGACACAAA+GGG - chr4.2:73174609-73174628 MS.gene043074:CDS 35.0%
AGCGAAGAAATGGCAATTAT+GGG - chr4.2:73174853-73174872 MS.gene043074:CDS 35.0%
CATGTTGGGATACCTATTAA+TGG - chr4.2:73174883-73174902 MS.gene043074:CDS 35.0%
CCTTCAAAGGTAGTAAATCA+GGG + chr4.2:73175005-73175024 None:intergenic 35.0%
GACCATAATTTGAAGGACAT+TGG - chr4.2:73174781-73174800 MS.gene043074:CDS 35.0%
TCCTTCAAAGGTAGTAAATC+AGG + chr4.2:73175006-73175025 None:intergenic 35.0%
TTTGTGTCAAGAAAGTCTCT+TGG + chr4.2:73174609-73174628 None:intergenic 35.0%
!! ATTATTGATGGACTCAATGG+TGG - chr4.2:73174927-73174946 MS.gene043074:CDS 35.0%
ATTATGGGTCATGGACATGT+TGG - chr4.2:73174868-73174887 MS.gene043074:CDS 40.0%
CCAGAATTGATCTCACCAAA+GGG + chr4.2:73174976-73174995 None:intergenic 40.0%
CCCTGATTTACTACCTTTGA+AGG - chr4.2:73175002-73175021 MS.gene043074:CDS 40.0%
GAAATGGCAATTATGGGTCA+TGG - chr4.2:73174859-73174878 MS.gene043074:CDS 40.0%
GAAGGACATTGGTAGACATT+TGG - chr4.2:73174792-73174811 MS.gene043074:CDS 40.0%
GAGCGAAGAAATGGCAATTA+TGG - chr4.2:73174852-73174871 MS.gene043074:CDS 40.0%
TCATTGTCAATGATGCTGCA+AGG - chr4.2:73174380-73174399 MS.gene043074:CDS 40.0%
TCCAGAATTGATCTCACCAA+AGG + chr4.2:73174977-73174996 None:intergenic 40.0%
TGCAGCATCATTGACAATGA+AGG + chr4.2:73174380-73174399 None:intergenic 40.0%
TTATGGGTCATGGACATGTT+GGG - chr4.2:73174869-73174888 MS.gene043074:CDS 40.0%
!! CGTCATGAATTTTGAGAGCA+CGG + chr4.2:73174551-73174570 None:intergenic 40.0%
!! TGAATTTTGAGAGCACGGAT+TGG + chr4.2:73174546-73174565 None:intergenic 40.0%
AGCATCTAGGAGCGAAGAAA+TGG - chr4.2:73174843-73174862 MS.gene043074:CDS 45.0%
ATCTCTGGTTGAGGCTCATA+AGG + chr4.2:73174502-73174521 None:intergenic 45.0%
ATGAGCCTCAACCAGAGATT+TGG - chr4.2:73174503-73174522 MS.gene043074:CDS 45.0%
CCCTTTGGTGAGATCAATTC+TGG - chr4.2:73174973-73174992 MS.gene043074:CDS 45.0%
CTCATAAGGGCCATACACTA+TGG + chr4.2:73174488-73174507 None:intergenic 45.0%
GGACATTGGTAGACATTTGG+CGG - chr4.2:73174795-73174814 MS.gene043074:CDS 45.0%
TCTCTGGTTGAGGCTCATAA+GGG + chr4.2:73174501-73174520 None:intergenic 45.0%
! CTTGACACAAAGGGTTCTGA+AGG - chr4.2:73174618-73174637 MS.gene043074:CDS 45.0%
ACAGGCTTCAATGCCACAGA+GGG + chr4.2:73174464-73174483 None:intergenic 50.0%
GAAGCCTGTGCCATAGTGTA+TGG - chr4.2:73174475-73174494 MS.gene043074:CDS 50.0%
GAAGGCCAAATCTCTGGTTG+AGG + chr4.2:73174511-73174530 None:intergenic 50.0%
TATCACCTCCAGCAGCATCT+AGG - chr4.2:73174830-73174849 MS.gene043074:CDS 50.0%
TCTGGTGAAGGCCAAATCTC+TGG + chr4.2:73174517-73174536 None:intergenic 50.0%
! AGAGATTTGGCCTTCACCAG+AGG - chr4.2:73174516-73174535 MS.gene043074:CDS 50.0%
! AGATTTGGCCTTCACCAGAG+GGG - chr4.2:73174518-73174537 MS.gene043074:CDS 50.0%
! GAGATTTGGCCTTCACCAGA+GGG - chr4.2:73174517-73174536 MS.gene043074:CDS 50.0%
!! TTGATGGACTCAATGGTGGC+TGG - chr4.2:73174931-73174950 MS.gene043074:CDS 50.0%
AGGGCCATACACTATGGCAC+AGG + chr4.2:73174482-73174501 None:intergenic 55.0%
CACAGGCTTCAATGCCACAG+AGG + chr4.2:73174465-73174484 None:intergenic 55.0%
GATGCTGCTGGAGGTGATAG+TGG + chr4.2:73174829-73174848 None:intergenic 55.0%
GCTGAACTTAGCACCCTCTG+TGG - chr4.2:73174448-73174467 MS.gene043074:CDS 55.0%
GTGCAAGAGACAGTGCCCTT+TGG - chr4.2:73174958-73174977 MS.gene043074:CDS 55.0%
TCTTCGCTCCTAGATGCTGC+TGG + chr4.2:73174841-73174860 None:intergenic 55.0%
! GATTTGGCCTTCACCAGAGG+GGG - chr4.2:73174519-73174538 MS.gene043074:CDS 55.0%
GATTGGACCCCCTCTGGTGA+AGG + chr4.2:73174529-73174548 None:intergenic 60.0%
TCGCTCCTAGATGCTGCTGG+AGG + chr4.2:73174838-73174857 None:intergenic 60.0%
!! AGCACGGATTGGACCCCCTC+TGG + chr4.2:73174535-73174554 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 73174352 73175041 73174352 ID=MS.gene043074
chr4.2 mRNA 73174352 73175041 73174352 ID=MS.gene043074.t1;Parent=MS.gene043074
chr4.2 exon 73174352 73175041 73174352 ID=MS.gene043074.t1.exon1;Parent=MS.gene043074.t1
chr4.2 CDS 73174352 73175041 73174352 ID=cds.MS.gene043074.t1;Parent=MS.gene043074.t1
Gene Sequence

>MS.gene043074

ATGGCTAGAAGTAAGGTTAAACTTGCCTTCATTGTCAATGATGCTGCAAGGAAAGAATCATACAAGAAAAGAAGAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCCCTTATGAGCCTCAACCAGAGATTTGGCCTTCACCAGAGGGGGTCCAATCCGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGAGAGCAAGAGATGACAATACTCATGTATCATTGTCTCAATGAAGGTAGAGTTGTGCACAACAATATTTCAATGGTTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATTTGAAGGACATTGGTAGACATTTGGCGGCAACAGTGCCACTATCACCTCCAGCAGCATCTAGGAGCGAAGAAATGGCAATTATGGGTCATGGACATGTTGGGATACCTATTAATGGTGATGACATTTTGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCAAGAGACAGTGCCCTTTGGTGAGATCAATTCTGGAGTTTGCCCTGATTTACTACCTTTGAAGGATCTTTTCTCAATTTAG
Protein sequence

>MS.gene043074.t1

MARSKVKLAFIVNDAARKESYKKRRKGLLKKVAELSTLCGIEACAIVYGPYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMMNQETFLTQRVLKAEEKLKKQRKENREQEMTILMYHCLNEGRVVHNNISMVDLSYLTWLIDHNLKDIGRHLAATVPLSPPAASRSEEMAIMGHGHVGIPINGDDILQSQLLMDSMVAGNVQETVPFGEINSGVCPDLLPLKDLFSI