AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043077

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043077.t1 MTR_4g031910 82.231 242 28 1 4 245 3 229 2.17e-135 380
MS.gene043077.t1 MTR_4g032290 79.752 242 36 1 4 245 3 231 9.94e-131 369
MS.gene043077.t1 MTR_8g036130 61.355 251 82 5 4 245 3 247 8.78e-98 286
MS.gene043077.t1 MTR_4g032620 57.959 245 89 4 1 245 1 231 6.05e-94 275
MS.gene043077.t1 MTR_4g032260 80.519 154 30 0 4 157 3 156 7.64e-80 237
MS.gene043077.t1 MTR_3g065100 48.548 241 117 4 4 242 3 238 2.63e-62 195
MS.gene043077.t1 MTR_3g466980 54.248 153 69 1 4 156 3 154 5.91e-57 181
MS.gene043077.t1 MTR_4g032270 55.629 151 46 2 95 245 2 131 5.99e-51 162
MS.gene043077.t1 MTR_2g035610 72.500 120 33 0 4 123 3 122 7.57e-49 157
MS.gene043077.t1 MTR_3g466830 43.478 161 90 1 4 164 2 161 4.81e-42 141
MS.gene043077.t1 MTR_5g075380 44.000 150 83 1 6 155 5 153 3.58e-40 136
MS.gene043077.t1 MTR_4g063790 40.491 163 97 0 4 166 3 165 7.10e-40 135
MS.gene043077.t1 MTR_3g466890 40.881 159 93 1 4 162 2 159 1.01e-37 130
MS.gene043077.t1 MTR_3g467080 39.610 154 93 0 4 157 3 156 2.00e-37 129
MS.gene043077.t1 MTR_5g047580 44.156 154 84 2 4 157 3 154 4.31e-37 128
MS.gene043077.t1 MTR_3g031100 40.764 157 92 1 1 157 1 156 1.07e-36 130
MS.gene043077.t1 MTR_2g016210 40.132 152 91 0 6 157 5 156 3.08e-36 128
MS.gene043077.t1 MTR_1g077390 40.260 154 90 2 4 157 3 154 7.93e-33 117
MS.gene043077.t1 MTR_1g090783 38.312 154 93 2 4 157 2 153 2.23e-32 116
MS.gene043077.t1 MTR_1g090710 38.365 159 96 2 1 159 1 157 7.47e-32 114
MS.gene043077.t1 MTR_1g090697 38.462 156 94 2 4 159 3 156 1.88e-31 114
MS.gene043077.t1 MTR_3g465410 39.416 137 83 0 30 166 1 137 5.61e-31 112
MS.gene043077.t1 MTR_1g084950 42.857 154 86 2 4 157 3 154 4.59e-30 110
MS.gene043077.t1 MTR_1g077320 41.558 154 88 2 4 157 3 154 7.40e-30 109
MS.gene043077.t1 MTR_7g011950 41.558 154 88 2 4 157 3 154 7.56e-30 109
MS.gene043077.t1 MTR_4g028800 77.273 66 13 1 6 71 5 68 6.84e-29 104
MS.gene043077.t1 MTR_4g028720 40.260 154 90 2 4 157 3 154 2.68e-28 105
MS.gene043077.t1 MTR_1g077300 40.881 159 90 2 4 162 3 157 6.92e-27 102
MS.gene043077.t1 MTR_3g031240 32.704 159 106 1 1 159 1 158 7.29e-27 102
MS.gene043077.t1 MTR_3g466900 36.923 130 81 1 33 162 2 130 7.85e-27 100
MS.gene043077.t1 MTR_7g055800 49.573 117 31 2 4 120 3 91 9.69e-27 99.4
MS.gene043077.t1 MTR_4g019670 38.462 156 80 2 3 158 5 144 5.98e-25 103
MS.gene043077.t1 MTR_7g106510 37.821 156 81 2 3 158 5 144 6.21e-25 102
MS.gene043077.t1 MTR_7g055790 53.211 109 33 1 4 112 61 151 1.15e-24 95.9
MS.gene043077.t1 MTR_3g466930 34.615 130 84 1 33 162 2 130 1.21e-24 95.5
MS.gene043077.t1 MTR_3g093900 29.016 193 126 2 3 195 5 186 1.51e-24 102
MS.gene043077.t1 MTR_2g035590 58.763 97 39 1 75 170 1 97 3.62e-24 93.2
MS.gene043077.t1 MTR_2g035580 33.775 151 99 1 7 157 6 155 1.97e-21 87.8
MS.gene043077.t1 MTR_7g055940 48.077 104 26 2 4 107 3 78 4.59e-21 84.3
MS.gene043077.t1 MTR_5g047560 40.870 115 51 2 4 118 3 100 5.62e-19 80.9
MS.gene043077.t1 MTR_4g084780 57.692 52 22 0 75 126 1 52 1.43e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043077.t1 AT5G48670 52.532 158 74 1 4 161 3 159 1.37e-43 150
MS.gene043077.t1 AT1G65300 39.674 184 97 3 4 187 2 171 2.76e-39 137
MS.gene043077.t1 AT5G26630 41.872 203 101 3 4 204 3 190 1.34e-38 134
MS.gene043077.t1 AT1G65330 40.000 185 97 2 4 188 2 172 3.15e-38 135
MS.gene043077.t1 AT5G26650 39.634 164 98 1 6 169 3 165 7.22e-35 128
MS.gene043077.t1 AT5G27960 39.024 164 99 1 6 169 3 165 4.13e-34 125
MS.gene043077.t1 AT1G31630 34.066 182 105 3 4 185 2 168 1.32e-32 121
MS.gene043077.t1 AT1G31640 37.748 151 92 2 4 154 2 150 1.18e-29 115
MS.gene043077.t1 AT5G27810 53.763 93 43 0 30 122 1 93 5.60e-29 106
MS.gene043077.t1 AT3G05860 37.838 148 91 1 4 151 3 149 9.08e-28 105
MS.gene043077.t1 AT3G05860 37.838 148 91 1 4 151 3 149 2.84e-27 105
MS.gene043077.t1 AT3G05860 37.838 148 91 1 4 151 3 149 2.93e-27 105
MS.gene043077.t1 AT2G28700 35.152 165 102 2 4 166 3 164 9.10e-26 103
MS.gene043077.t1 AT5G26580 35.256 156 85 3 1 156 1 140 7.18e-24 98.6
MS.gene043077.t1 AT1G22590 31.210 157 103 2 1 157 1 152 2.02e-23 93.2
MS.gene043077.t1 AT5G06500 37.500 120 75 0 4 123 3 122 8.49e-19 82.8
MS.gene043077.t1 AT2G40210 39.098 133 79 2 4 135 3 134 2.79e-17 80.5
MS.gene043077.t1 AT5G58890 37.795 127 68 5 7 124 6 130 2.09e-14 71.6
MS.gene043077.t1 AT5G55690 41.772 79 38 3 1 72 1 78 6.25e-11 61.6
MS.gene043077.t1 AT5G55690 41.772 79 38 3 1 72 1 78 6.25e-11 61.6

Find 62 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACAAAGAAAAGAAAAT+AGG 0.231520 4.2:-73147637 MS.gene043077:CDS
GAGTGAAGAAATGGCAATTA+TGG 0.233693 4.2:-73147375 MS.gene043077:CDS
CCATTTGGTGAGGTCAATTC+TGG 0.238528 4.2:-73147254 MS.gene043077:CDS
TCAATTTCTCTTCAGCCTTC+AGG 0.259829 4.2:+73147660 None:intergenic
AACGAAGGGTCTATTGAAAA+AGG 0.335070 4.2:-73147870 MS.gene043077:CDS
ATGAGCCTCAACCTGAGATT+TGG 0.345571 4.2:-73147790 MS.gene043077:CDS
ATAATGTCATCATCATTAAT+AGG 0.375800 4.2:+73147332 None:intergenic
GAAGCCTTTGCCATAGTGTA+TGG 0.388094 4.2:-73147818 MS.gene043077:CDS
TCGCCATTTCGAGTTGGACT+TGG 0.402057 4.2:+73147432 None:intergenic
TATGCAAAGTCAATTATTGA+TGG 0.408286 4.2:-73147312 MS.gene043077:CDS
ATCTCAGGTTGAGGCTCATA+AGG 0.418788 4.2:+73147794 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4.2:-73147636 MS.gene043077:CDS
GTGAGGTCAATTCTGGACTT+TGG 0.420430 4.2:-73147247 MS.gene043077:CDS
AGTGAAGAAATGGCAATTAT+GGG 0.424565 4.2:-73147374 MS.gene043077:CDS
GAAGGATGTTAGTAGACGTT+TGG 0.425640 4.2:-73147501 MS.gene043077:CDS
GGTCTGATTTACTACCTTGA+AGG 0.436579 4.2:-73147226 None:intergenic
AAACCAAGTCCAACTCGAAA+TGG 0.452175 4.2:-73147435 MS.gene043077:CDS
AGCATCTAGGAGTGAAGAAA+TGG 0.457574 4.2:-73147384 MS.gene043077:CDS
AACCTTACTTCTTCTACCCA+TGG 0.464315 4.2:+73147946 None:intergenic
CTCATAAGGTCCATACACTA+TGG 0.467279 4.2:+73147808 None:intergenic
TTATGGGTCATGGCCATGTT+GGG 0.476409 4.2:-73147358 MS.gene043077:CDS
TCAATTATTGATGGACTCAA+TGG 0.482426 4.2:-73147303 MS.gene043077:CDS
GTTGATTGACCACAATTTGA+AGG 0.487410 4.2:-73147519 MS.gene043077:CDS
TCTGGTAAAGGCCAAATCTC+AGG 0.493519 4.2:+73147779 None:intergenic
AACCATGGGTAGAAGAAGTA+AGG 0.501192 4.2:-73147948 None:intergenic
TTAGTAGACGTTTGGAAGCA+TGG 0.506023 4.2:-73147493 MS.gene043077:CDS
CCAGAATTGACCTCACCAAA+TGG 0.507378 4.2:+73147254 None:intergenic
GATTGGACACCCTCTGGTAA+AGG 0.514238 4.2:+73147767 None:intergenic
TGCAACATCATTGACAATGA+AGG 0.517037 4.2:+73147916 None:intergenic
ATGAGTCAATGTCTTAATGA+AGG 0.517500 4.2:-73147593 MS.gene043077:CDS
CAATGATCAAAATCAAATCA+TGG 0.521352 4.2:-73147465 MS.gene043077:CDS
AGCACAGATTGGACACCCTC+TGG 0.522070 4.2:+73147761 None:intergenic
CACCACTTCTAGCAGCATCT+AGG 0.525777 4.2:-73147397 MS.gene043077:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.525855 4.2:+73147687 None:intergenic
TGGATATGAGTTATGTTGCT+TGG 0.532880 4.2:-73147541 MS.gene043077:CDS
AGAGACTTTCTTGACACAAA+GGG 0.533444 4.2:-73147684 MS.gene043077:CDS
GTGCAAGAGACAGTGCCATT+TGG 0.542398 4.2:-73147269 MS.gene043077:CDS
TACTAACATCCTTCAAATTG+TGG 0.547844 4.2:+73147510 None:intergenic
GAAATGGCAATTATGGGTCA+TGG 0.549964 4.2:-73147368 MS.gene043077:CDS
TAGTAGACGTTTGGAAGCAT+GGG 0.553921 4.2:-73147492 MS.gene043077:CDS
GAGATTTGGCCTTTACCAGA+GGG 0.563951 4.2:-73147776 MS.gene043077:CDS
ATTATGGGTCATGGCCATGT+TGG 0.565284 4.2:-73147359 MS.gene043077:CDS
CTTGACACAAAGGGTCCTGA+AGG 0.570745 4.2:-73147675 MS.gene043077:CDS
CTCCTAGATGCTGCTAGAAG+TGG 0.571630 4.2:+73147395 None:intergenic
TTGTTGTCGCCATTTCGAGT+TGG 0.584691 4.2:+73147426 None:intergenic
CATTAATAGGTATCCCAACA+TGG 0.589301 4.2:+73147345 None:intergenic
TCATTGTCAATGATGTTGCA+AGG 0.595963 4.2:-73147913 MS.gene043077:CDS
AAAGGCCAAATCTCAGGTTG+AGG 0.605277 4.2:+73147785 None:intergenic
CTAGATGCTGCTAGAAGTGG+TGG 0.605478 4.2:+73147398 None:intergenic
TTGATGGACTCAATGGTGGC+TGG 0.607204 4.2:-73147296 MS.gene043077:CDS
AAAGGCTTCAATGCCACAGA+GGG 0.618725 4.2:+73147832 None:intergenic
ATCATACAAGAAAAGAACGA+AGG 0.618898 4.2:-73147885 MS.gene043077:CDS
TCATACAAGAAAAGAACGAA+GGG 0.620354 4.2:-73147884 MS.gene043077:CDS
AAGAGACTTTCTTGACACAA+AGG 0.620991 4.2:-73147685 MS.gene043077:CDS
TGAGATTTGGCCTTTACCAG+AGG 0.625137 4.2:-73147777 MS.gene043077:CDS
GATGCTGCTAGAAGTGGTGG+TGG 0.633563 4.2:+73147401 None:intergenic
AGGTCCATACACTATGGCAA+AGG 0.638234 4.2:+73147814 None:intergenic
AGAGACAGTGCCATTTGGTG+AGG 0.639188 4.2:-73147264 MS.gene043077:CDS
ATTATTGATGGACTCAATGG+TGG 0.666274 4.2:-73147300 MS.gene043077:CDS
GCATGACAATATGTCAACAA+TGG 0.671130 4.2:-73147561 MS.gene043077:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4.2:-73147845 MS.gene043077:CDS
CAAAGGCTTCAATGCCACAG+AGG 0.706392 4.2:+73147831 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAACAAAGAAAAGAAAAT+AGG - chr4.2:73147536-73147555 MS.gene043077:CDS 20.0%
!! ATAATGTCATCATCATTAAT+AGG + chr4.2:73147844-73147863 None:intergenic 20.0%
!! GAAACAAAGAAAAGAAAATA+GGG - chr4.2:73147537-73147556 MS.gene043077:CDS 20.0%
! CAATGATCAAAATCAAATCA+TGG - chr4.2:73147708-73147727 MS.gene043077:CDS 25.0%
! TATGCAAAGTCAATTATTGA+TGG - chr4.2:73147861-73147880 MS.gene043077:CDS 25.0%
AGTGAAGAAATGGCAATTAT+GGG - chr4.2:73147799-73147818 MS.gene043077:CDS 30.0%
ATCATACAAGAAAAGAACGA+AGG - chr4.2:73147288-73147307 MS.gene043077:CDS 30.0%
ATGAGTCAATGTCTTAATGA+AGG - chr4.2:73147580-73147599 MS.gene043077:CDS 30.0%
TACTAACATCCTTCAAATTG+TGG + chr4.2:73147666-73147685 None:intergenic 30.0%
TCATACAAGAAAAGAACGAA+GGG - chr4.2:73147289-73147308 MS.gene043077:CDS 30.0%
!! TCAATTATTGATGGACTCAA+TGG - chr4.2:73147870-73147889 MS.gene043077:CDS 30.0%
AACGAAGGGTCTATTGAAAA+AGG - chr4.2:73147303-73147322 MS.gene043077:CDS 35.0%
AAGAGACTTTCTTGACACAA+AGG - chr4.2:73147488-73147507 MS.gene043077:CDS 35.0%
AGAGACTTTCTTGACACAAA+GGG - chr4.2:73147489-73147508 MS.gene043077:CDS 35.0%
CATTAATAGGTATCCCAACA+TGG + chr4.2:73147831-73147850 None:intergenic 35.0%
GAGTGAAGAAATGGCAATTA+TGG - chr4.2:73147798-73147817 MS.gene043077:CDS 35.0%
GCATGACAATATGTCAACAA+TGG - chr4.2:73147612-73147631 MS.gene043077:CDS 35.0%
GTTGATTGACCACAATTTGA+AGG - chr4.2:73147654-73147673 MS.gene043077:CDS 35.0%
TCATTGTCAATGATGTTGCA+AGG - chr4.2:73147260-73147279 MS.gene043077:CDS 35.0%
TGCAACATCATTGACAATGA+AGG + chr4.2:73147260-73147279 None:intergenic 35.0%
TGGATATGAGTTATGTTGCT+TGG - chr4.2:73147632-73147651 MS.gene043077:CDS 35.0%
TTTGTGTCAAGAAAGTCTCT+TGG + chr4.2:73147489-73147508 None:intergenic 35.0%
!! ATTATTGATGGACTCAATGG+TGG - chr4.2:73147873-73147892 MS.gene043077:CDS 35.0%
!! TGAATTTTGAGAGCACAGAT+TGG + chr4.2:73147426-73147445 None:intergenic 35.0%
AAACCAAGTCCAACTCGAAA+TGG - chr4.2:73147738-73147757 MS.gene043077:CDS 40.0%
AGCATCTAGGAGTGAAGAAA+TGG - chr4.2:73147789-73147808 MS.gene043077:CDS 40.0%
CTCATAAGGTCCATACACTA+TGG + chr4.2:73147368-73147387 None:intergenic 40.0%
GAAATGGCAATTATGGGTCA+TGG - chr4.2:73147805-73147824 MS.gene043077:CDS 40.0%
GAAGGATGTTAGTAGACGTT+TGG - chr4.2:73147672-73147691 MS.gene043077:CDS 40.0%
TCAATTTCTCTTCAGCCTTC+AGG + chr4.2:73147516-73147535 None:intergenic 40.0%
TTAGTAGACGTTTGGAAGCA+TGG - chr4.2:73147680-73147699 MS.gene043077:CDS 40.0%
! TAGTAGACGTTTGGAAGCAT+GGG - chr4.2:73147681-73147700 MS.gene043077:CDS 40.0%
AAAGGCTTCAATGCCACAGA+GGG + chr4.2:73147344-73147363 None:intergenic 45.0%
AGGTCCATACACTATGGCAA+AGG + chr4.2:73147362-73147381 None:intergenic 45.0%
ATGAGCCTCAACCTGAGATT+TGG - chr4.2:73147383-73147402 MS.gene043077:CDS 45.0%
ATTATGGGTCATGGCCATGT+TGG - chr4.2:73147814-73147833 MS.gene043077:CDS 45.0%
CCAGAATTGACCTCACCAAA+TGG + chr4.2:73147922-73147941 None:intergenic 45.0%
GAAGCCTTTGCCATAGTGTA+TGG - chr4.2:73147355-73147374 MS.gene043077:CDS 45.0%
GTGAGGTCAATTCTGGACTT+TGG - chr4.2:73147926-73147945 MS.gene043077:CDS 45.0%
TCTGGTAAAGGCCAAATCTC+AGG + chr4.2:73147397-73147416 None:intergenic 45.0%
TTATGGGTCATGGCCATGTT+GGG - chr4.2:73147815-73147834 MS.gene043077:CDS 45.0%
! AAAGGCCAAATCTCAGGTTG+AGG + chr4.2:73147391-73147410 None:intergenic 45.0%
! CCATTTGGTGAGGTCAATTC+TGG - chr4.2:73147919-73147938 MS.gene043077:CDS 45.0%
! GAGATTTGGCCTTTACCAGA+GGG - chr4.2:73147397-73147416 MS.gene043077:CDS 45.0%
! TGAGATTTGGCCTTTACCAG+AGG - chr4.2:73147396-73147415 MS.gene043077:CDS 45.0%
! TTGTTGTCGCCATTTCGAGT+TGG + chr4.2:73147750-73147769 None:intergenic 45.0%
!! ATCTCAGGTTGAGGCTCATA+AGG + chr4.2:73147382-73147401 None:intergenic 45.0%
CAAAGGCTTCAATGCCACAG+AGG + chr4.2:73147345-73147364 None:intergenic 50.0%
CACCACTTCTAGCAGCATCT+AGG - chr4.2:73147776-73147795 MS.gene043077:CDS 50.0%
CTAGATGCTGCTAGAAGTGG+TGG + chr4.2:73147778-73147797 None:intergenic 50.0%
CTCCTAGATGCTGCTAGAAG+TGG + chr4.2:73147781-73147800 None:intergenic 50.0%
CTTGACACAAAGGGTCCTGA+AGG - chr4.2:73147498-73147517 MS.gene043077:CDS 50.0%
GATTGGACACCCTCTGGTAA+AGG + chr4.2:73147409-73147428 None:intergenic 50.0%
! GTGCAAGAGACAGTGCCATT+TGG - chr4.2:73147904-73147923 MS.gene043077:CDS 50.0%
!! AGAGACAGTGCCATTTGGTG+AGG - chr4.2:73147909-73147928 MS.gene043077:CDS 50.0%
!! TCGCCATTTCGAGTTGGACT+TGG + chr4.2:73147744-73147763 None:intergenic 50.0%
!! TTGATGGACTCAATGGTGGC+TGG - chr4.2:73147877-73147896 MS.gene043077:CDS 50.0%
AGCACAGATTGGACACCCTC+TGG + chr4.2:73147415-73147434 None:intergenic 55.0%
GATGCTGCTAGAAGTGGTGG+TGG + chr4.2:73147775-73147794 None:intergenic 55.0%
GCTGAACTTAGCACCCTCTG+TGG - chr4.2:73147328-73147347 MS.gene043077:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 73147229 73147966 73147229 ID=MS.gene043077
chr4.2 mRNA 73147229 73147966 73147229 ID=MS.gene043077.t1;Parent=MS.gene043077
chr4.2 exon 73147229 73147966 73147229 ID=MS.gene043077.t1.exon1;Parent=MS.gene043077.t1
chr4.2 CDS 73147229 73147966 73147229 ID=cds.MS.gene043077.t1;Parent=MS.gene043077.t1
Gene Sequence

>MS.gene043077

ATGGGTAGAAGAAGTAAGGTTAAACTTGCCTTCATTGTCAATGATGTTGCAAGGAAAGCATCATACAAGAAAAGAACGAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTTTGCCATAGTGTATGGACCTTATGAGCCTCAACCTGAGATTTGGCCTTTACCAGAGGGTGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTCCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATGAGTCAATGTCTTAATGAAGGTAAAGTTGTGCATGACAATATGTCAACAATGGATATGAGTTATGTTGCTTGGTTGATTGACCACAATTTGAAGGATGTTAGTAGACGTTTGGAAGCATGGGATAACAATGATCAAAATCAAATCATGGCTATTCAAAACCAAGTCCAACTCGAAATGGCGACAACAATACCACCACCACTTCTAGCAGCATCTAGGAGTGAAGAAATGGCAATTATGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGACATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCAAGAGACAGTGCCATTTGGTGAGGTCAATTCTGGACTTTGGTCTGATTTACTACCTTGA
Protein sequence

>MS.gene043077.t1

MGRRSKVKLAFIVNDVARKASYKKRTKGLLKKVAELSTLCGIEAFAIVYGPYEPQPEIWPLPEGVQSVLSKFMTMHEFQKCKKMMNQETFLTQRVLKAEEKLKKQRKENREQEMTILMSQCLNEGKVVHDNMSTMDMSYVAWLIDHNLKDVSRRLEAWDNNDQNQIMAIQNQVQLEMATTIPPPLLAASRSEEMAIMGHGHVGIPINDDDIMQSQLLMDSMVAGNVQETVPFGEVNSGLWSDLLP