AlfalfaGEDB | MS.gene04331

M. sativa cultivar XinJiangDaYe / MS.gene04331

KEGG: K20537
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04331.t1	PNY16655.1	59.5	126	18	2	6	99	71	195	1.00E-31	146

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04331.t1	Q5Z859	55.6	126	23	2	6	99	91	215	8.6e-32	137.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04331.t1	A0A2K3PMY9	59.5	126	18	2	6	99	71	195	7.2e-32	146.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04331.t1	MTR_3g460950	57.937	126	20	2	6	99	91	215	2.74e-42	142
MS.gene04331.t1	MTR_3g060330	57.937	126	20	2	6	99	91	215	2.74e-42	142
MS.gene04331.t1	MTR_8g086000	56.349	126	22	2	6	99	91	215	2.78e-40	137
MS.gene04331.t1	MTR_8g086000	56.349	126	22	2	6	99	134	258	3.68e-40	137
MS.gene04331.t1	MTR_3g460960	67.000	100	18	2	6	105	96	180	2.72e-38	129
MS.gene04331.t1	MTR_3g112210	53.968	126	25	2	6	99	91	215	1.12e-37	130
MS.gene04331.t1	MTR_8g086010	60.440	91	34	1	8	96	192	282	4.99e-33	119
MS.gene04331.t1	MTR_7g078690	45.038	131	38	3	1	99	92	220	1.98e-27	103
MS.gene04331.t1	MTR_5g010030	43.511	131	40	3	1	99	95	223	2.03e-27	103
MS.gene04331.t1	MTR_8g098845	43.511	131	40	3	1	99	106	234	6.75e-27	102
MS.gene04331.t1	MTR_8g028720	42.748	131	41	3	1	99	91	219	1.36e-26	100
MS.gene04331.t1	MTR_4g061320	42.748	131	41	3	1	99	94	222	6.28e-26	99.4
MS.gene04331.t1	MTR_4g087620	39.695	131	45	3	1	99	109	237	6.36e-25	95.1
MS.gene04331.t1	MTR_4g087620	39.695	131	45	3	1	99	109	237	1.44e-24	95.5
MS.gene04331.t1	MTR_3g060390	44.186	129	22	4	3	99	71	181	2.72e-23	91.7
MS.gene04331.t1	MTR_4g061130	40.476	126	41	3	6	99	98	221	2.00e-22	89.7
MS.gene04331.t1	MTR_3g060350	49.485	97	38	2	6	99	96	184	1.16e-21	86.3
MS.gene04331.t1	MTR_3g460980	37.879	132	30	4	3	99	71	185	3.58e-18	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04331.t1	AT1G59580	54.762	126	24	2	6	99	91	215	5.13e-39	134
MS.gene04331.t1	AT1G59580	54.762	126	24	2	6	99	91	215	5.13e-39	134
MS.gene04331.t1	AT2G18170	54.762	126	24	2	6	99	91	215	1.36e-38	132
MS.gene04331.t1	AT1G10210	54.762	126	24	2	6	99	91	215	1.02e-37	130
MS.gene04331.t1	AT1G10210	54.762	126	24	2	6	99	91	215	1.02e-37	130
MS.gene04331.t1	AT1G10210	54.762	126	24	2	6	99	91	215	1.02e-37	130
MS.gene04331.t1	AT4G36450	50.794	126	26	3	6	99	91	212	7.92e-33	117
MS.gene04331.t1	AT1G07880	46.032	126	34	3	6	99	92	215	1.25e-28	104
MS.gene04331.t1	AT1G07880	45.038	131	38	3	1	99	87	215	4.81e-28	104
MS.gene04331.t1	AT4G01370	42.748	131	41	3	1	99	97	225	1.60e-27	103
MS.gene04331.t1	AT1G01560	42.857	126	38	3	6	99	99	222	1.19e-25	97.1
MS.gene04331.t1	AT1G01560	42.857	126	38	3	6	99	99	222	1.19e-25	97.1
MS.gene04331.t1	AT1G01560	42.857	126	38	3	6	99	99	222	2.25e-25	97.4
MS.gene04331.t1	AT1G01560	42.857	126	38	3	6	99	99	222	3.56e-25	97.1
MS.gene04331.t1	AT2G43790	38.931	131	46	3	1	99	117	245	7.42e-25	96.7
MS.gene04331.t1	AT4G11330	42.857	126	38	3	6	99	102	225	3.34e-24	94.7
MS.gene04331.t1	AT2G46070	40.476	126	41	3	6	99	100	223	4.71e-23	90.9
MS.gene04331.t1	AT2G46070	39.695	131	45	3	1	99	95	223	8.26e-23	90.9
MS.gene04331.t1	AT2G46070	39.695	131	45	3	1	99	129	257	9.37e-23	90.9
MS.gene04331.t1	AT3G45640	40.476	126	41	3	6	99	97	220	1.26e-22	90.1
MS.gene04331.t1	AT3G59790	36.508	126	46	3	6	99	119	242	5.51e-20	83.2

Find 21 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACCATTGCCGATATTTCT+TGG	0.281243	3.4:-56313041	MS.gene04331:CDS
CGGCAATGGTCATTGGAAAT+TGG	0.300842	3.4:+56313052	None:intergenic
GCTCGGACCAATCAAGTTAA+TGG	0.324476	3.4:-56312514	MS.gene04331:CDS
AATCTCGCCATTAACTTGAT+TGG	0.360614	3.4:+56312507	None:intergenic
CTATTAGCATGTGACAATTA+CGG	0.396118	3.4:-56312439	MS.gene04331:CDS
GAAATATCGGCAATGGTCAT+TGG	0.411829	3.4:+56313045	None:intergenic
CTACTTTGTCTATGAACTCA+TGG	0.455179	3.4:-56313110	MS.gene04331:CDS
GTACCTGCGCCAAGAAATAT+CGG	0.461903	3.4:+56313032	None:intergenic
TCACATGCTAATAGAAGCTC+TGG	0.477513	3.4:+56312448	None:intergenic
TAAAGTTGCTTAGACATGTC+CGG	0.526853	3.4:-56313199	None:intergenic
TTGCCGATATTTCTTGGCGC+AGG	0.547464	3.4:-56313035	MS.gene04331:intron
CAAAGTGATCACATATGTGC+CGG	0.561565	3.4:+56313180	None:intergenic
GCAAGAATGCATCCCACGGA+TGG	0.565016	3.4:+56312411	None:intergenic
TATGTGACTTCGGACTTGCT+CGG	0.583313	3.4:-56312531	MS.gene04331:CDS
GCGCCAAGAAATATCGGCAA+TGG	0.597426	3.4:+56313038	None:intergenic
CAGAGTATATTGTCACGCGT+TGG	0.604813	3.4:-56312480	MS.gene04331:CDS
CTCAGCAAGAATGCATCCCA+CGG	0.610293	3.4:+56312407	None:intergenic
GTCATTGGAAATTGGCTCAG+AGG	0.613015	3.4:+56313060	None:intergenic
GATTTGAAGATATGTGACTT+CGG	0.636250	3.4:-56312541	MS.gene04331:intron
AATTACGGAACATCCATCCG+TGG	0.663559	3.4:-56312424	MS.gene04331:CDS
ATTACGGAACATCCATCCGT+GGG	0.671330	3.4:-56312423	MS.gene04331:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATTTTCAGTTATTTCTAT+TGG	-	chr3.4:56312598-56312617	MS.gene04331:intron	15.0%
!!	AGAAAATTATAGCTCATAAT+AGG	+	chr3.4:56312804-56312823	None:intergenic	20.0%
!!	ATTACTATTCCTATTATCAA+TGG	+	chr3.4:56312872-56312891	None:intergenic	20.0%
!!	TAGAAATAACTGAAAATATG+AGG	+	chr3.4:56312597-56312616	None:intergenic	20.0%
!	AAACTCAAATTAAAGGTAGT+TGG	-	chr3.4:56312638-56312657	MS.gene04331:intron	25.0%
!	AACTAGAGGATGAAAATTAT+TGG	-	chr3.4:56312701-56312720	MS.gene04331:intron	25.0%
!	TCAGTCAAAACTCAAATTAA+AGG	-	chr3.4:56312631-56312650	MS.gene04331:intron	25.0%
!	TTATAGAATGTATGAACTAG+AGG	-	chr3.4:56312687-56312706	MS.gene04331:intron	25.0%
!!!	CCAAAAAACAACTTGTTTTA+AGG	-	chr3.4:56312455-56312474	MS.gene04331:CDS	25.0%
!!!	CCTTAAAACAAGTTGTTTTT+TGG	+	chr3.4:56312458-56312477	None:intergenic	25.0%
	CTATTAGCATGTGACAATTA+CGG	-	chr3.4:56313153-56313172	MS.gene04331:CDS	30.0%
	GATTTGAAGATATGTGACTT+CGG	-	chr3.4:56313051-56313070	MS.gene04331:CDS	30.0%
	TCACATTAATTTCCCAACTT+GGG	+	chr3.4:56312727-56312746	None:intergenic	30.0%
	TCATTAATGAGCAAATTTGC+AGG	+	chr3.4:56313024-56313043	None:intergenic	30.0%
!	TGTTGATCACCATTGATAAT+AGG	-	chr3.4:56312860-56312879	MS.gene04331:intron	30.0%
!!!	CTCTTATTTTAGTTGCTTGA+AGG	-	chr3.4:56312952-56312971	MS.gene04331:intron	30.0%
	CTACTTTGTCTATGAACTCA+TGG	-	chr3.4:56312482-56312501	MS.gene04331:CDS	35.0%
	CTCACATTAATTTCCCAACT+TGG	+	chr3.4:56312728-56312747	None:intergenic	35.0%
	GAAAATTATTGGCCCAAGTT+GGG	-	chr3.4:56312712-56312731	MS.gene04331:intron	35.0%
	TGAAAATTATTGGCCCAAGT+TGG	-	chr3.4:56312711-56312730	MS.gene04331:intron	35.0%
!	AATCTCGCCATTAACTTGAT+TGG	+	chr3.4:56313088-56313107	None:intergenic	35.0%
	CAAAGTGATCACATATGTGC+CGG	+	chr3.4:56312415-56312434	None:intergenic	40.0%
	GAAATATCGGCAATGGTCAT+TGG	+	chr3.4:56312550-56312569	None:intergenic	40.0%
	TCACATGCTAATAGAAGCTC+TGG	+	chr3.4:56313147-56313166	None:intergenic	40.0%
	TGACCATTGCCGATATTTCT+TGG	-	chr3.4:56312551-56312570	MS.gene04331:intron	40.0%
	AATTACGGAACATCCATCCG+TGG	-	chr3.4:56313168-56313187	MS.gene04331:CDS	45.0%
	ATTACGGAACATCCATCCGT+GGG	-	chr3.4:56313169-56313188	MS.gene04331:CDS	45.0%
	CAGAGTATATTGTCACGCGT+TGG	-	chr3.4:56313112-56313131	MS.gene04331:CDS	45.0%
	CGGCAATGGTCATTGGAAAT+TGG	+	chr3.4:56312543-56312562	None:intergenic	45.0%
	GCTCGGACCAATCAAGTTAA+TGG	-	chr3.4:56313078-56313097	MS.gene04331:CDS	45.0%
	GTACCTGCGCCAAGAAATAT+CGG	+	chr3.4:56312563-56312582	None:intergenic	45.0%
	TGAGCAAATTTGCAGGCTTC+AGG	+	chr3.4:56313017-56313036	None:intergenic	45.0%
!	GTCATTGGAAATTGGCTCAG+AGG	+	chr3.4:56312535-56312554	None:intergenic	45.0%
!	TATGTGACTTCGGACTTGCT+CGG	-	chr3.4:56313061-56313080	MS.gene04331:CDS	45.0%
	GCGCCAAGAAATATCGGCAA+TGG	+	chr3.4:56312557-56312576	None:intergenic	50.0%
	TTGCCGATATTTCTTGGCGC+AGG	-	chr3.4:56312557-56312576	MS.gene04331:intron	50.0%
	GCAAGAATGCATCCCACGGA+TGG	+	chr3.4:56313184-56313203	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	56312408	56313206	56312408	ID=MS.gene04331
chr3.4	mRNA	56312408	56313206	56312408	ID=MS.gene04331.t1;Parent=MS.gene04331
chr3.4	exon	56313036	56313206	56313036	ID=MS.gene04331.t1.exon1;Parent=MS.gene04331.t1
chr3.4	CDS	56313036	56313206	56313036	ID=cds.MS.gene04331.t1;Parent=MS.gene04331.t1
chr3.4	exon	56312408	56312554	56312408	ID=MS.gene04331.t1.exon2;Parent=MS.gene04331.t1
chr3.4	CDS	56312408	56312554	56312408	ID=cds.MS.gene04331.t1;Parent=MS.gene04331.t1

Gene Sequence

>MS.gene04331

ATGTCCGGCACATATGTGATCACTTTGAAAGATGTTATGATACTAGACCAAAAAACAACTTGTTTTAAGGATGTCTACTTTGTCTATGAACTCATGGATACTGATCTTCATCATGTTATCAAGTCCTCTGAGCCAATTTCCAATGACCATTGCCGATATTTCTTGGCGCAGGTACTAATTTCAATTCCTCATATTTTCAGTTATTTCTATTGGCTTTACATTTTCAGTCAAAACTCAAATTAAAGGTAGTTGGTCTTGAATGAGTATACACTGTCATTCTTATAGAATGTATGAACTAGAGGATGAAAATTATTGGCCCAAGTTGGGAAATTAATGTGAGCTTAACATTGATTATTGATTAAAATAAAGTTCTTGTGAAAAGATTCAAAAGAGCCTATTATGAGCTATAATTTTCTTGTCTGCATTTTTTTAATATATGTTTTTGAGTATATTGTTGATCACCATTGATAATAGGAATAGTAATATACTAGTACAAAGCAATTCACTTAGTTTACGTATCTTTCTGATTGTTACTAATCATAAACTCTTATTTTAGTTGCTTGAAGGTCTTAATCATCTTCATTCTGCAAATATTCTTCATCGTGACCTGAAGCCTGCAAATTTGCTCATTAATGAAAATTGTGATTTGAAGATATGTGACTTCGGACTTGCTCGGACCAATCAAGTTAATGGCGAGATTATGACAGAGTATATTGTCACGCGTTGGTATCGTGCTCCAGAGCTTCTATTAGCATGTGACAATTACGGAACATCCATCCGTGGGATGCATTCTTGCTGA

Protein sequence

>MS.gene04331.t1

MSGTYVITLKDVMILDQKTTCFKDVYFVYELMDTDLHHVIKSSEPISNDHCRYFLAQICDFGLARTNQVNGEIMTEYIVTRWYRAPELLLACDNYGTSIRGMHSC