AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043315

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043315.t1 MTR_2g089100 99.346 459 2 1 1 459 1 458 0.0 940
MS.gene043315.t1 MTR_5g015950 43.448 435 198 8 46 446 28 448 5.60e-120 358
MS.gene043315.t1 MTR_3g053270 43.448 435 198 8 46 446 28 448 5.60e-120 358
MS.gene043315.t1 MTR_4g095500 41.772 474 219 9 13 446 14 470 3.14e-119 357
MS.gene043315.t1 MTR_4g097080 30.049 406 241 8 51 445 131 504 4.61e-59 202
MS.gene043315.t1 MTR_5g015490 31.618 408 250 7 45 445 106 491 4.01e-58 199
MS.gene043315.t1 MTR_2g099110 28.404 426 249 9 41 445 62 452 4.44e-48 171
MS.gene043315.t1 MTR_1g029420 29.798 396 248 7 51 443 223 591 2.43e-47 172
MS.gene043315.t1 MTR_5g094450 25.342 438 297 7 11 443 119 531 3.20e-44 162
MS.gene043315.t1 MTR_7g027190 28.261 414 245 10 47 443 294 672 5.40e-42 158
MS.gene043315.t1 MTR_5g097480 27.295 403 251 8 50 443 174 543 9.74e-42 156
MS.gene043315.t1 MTR_3g089055 27.047 403 250 8 51 443 196 564 7.94e-41 154
MS.gene043315.t1 MTR_3g089055 27.047 403 250 8 51 443 201 569 8.43e-41 154
MS.gene043315.t1 MTR_2g097410 26.521 411 261 9 46 446 364 743 1.10e-39 152
MS.gene043315.t1 MTR_2g082090 25.622 402 259 7 50 443 209 578 4.23e-39 149
MS.gene043315.t1 MTR_4g064160 27.340 406 254 9 50 446 313 686 5.87e-39 150
MS.gene043315.t1 MTR_3g056110 25.708 424 267 10 32 443 154 541 5.80e-38 145
MS.gene043315.t1 MTR_7g062120 28.293 410 245 10 50 446 369 742 6.59e-38 147
MS.gene043315.t1 MTR_4g064150 26.650 409 257 9 50 446 358 735 9.47e-38 146
MS.gene043315.t1 MTR_4g064200 28.288 403 246 9 53 446 284 652 2.17e-37 145
MS.gene043315.t1 MTR_4g064120 26.290 407 261 9 50 446 251 628 2.47e-36 142
MS.gene043315.t1 MTR_2g097310 27.517 447 253 13 26 446 239 640 5.89e-36 140
MS.gene043315.t1 MTR_4g102790 25.616 406 261 10 53 448 292 666 1.54e-35 140
MS.gene043315.t1 MTR_2g097473 26.368 402 254 9 53 446 289 656 1.67e-35 139
MS.gene043315.t1 MTR_4g064180 26.406 409 258 11 50 446 251 628 1.13e-34 137
MS.gene043315.t1 MTR_2g097467 25.935 401 257 9 53 446 289 656 3.14e-34 135
MS.gene043315.t1 MTR_4g133660 26.667 405 254 11 50 444 172 543 3.30e-34 135
MS.gene043315.t1 MTR_5g009080 24.943 441 274 9 47 443 50 477 5.38e-34 133
MS.gene043315.t1 MTR_2g097463 26.866 402 252 9 53 446 290 657 5.53e-34 135
MS.gene043315.t1 MTR_3g022830 25.708 424 267 10 54 443 26 435 1.25e-33 131
MS.gene043315.t1 MTR_3g065980 26.253 419 242 11 47 443 166 539 2.27e-33 132
MS.gene043315.t1 MTR_2g097350 27.073 410 248 10 50 446 271 642 8.21e-32 129
MS.gene043315.t1 MTR_4g104020 25.829 422 260 11 41 443 132 519 5.66e-31 125
MS.gene043315.t1 MTR_2g026250 26.303 403 256 9 50 443 225 595 8.79e-31 125
MS.gene043315.t1 MTR_8g020840 25.728 412 264 11 46 443 164 547 3.05e-30 123
MS.gene043315.t1 MTR_8g442410 26.116 448 282 11 8 443 114 524 7.43e-30 122
MS.gene043315.t1 MTR_4g076140 23.624 436 285 9 47 443 42 468 8.32e-30 121
MS.gene043315.t1 MTR_2g097390 26.098 410 252 9 50 446 272 643 4.99e-29 120
MS.gene043315.t1 MTR_4g076020 24.000 400 252 10 56 452 87 437 9.23e-29 118
MS.gene043315.t1 MTR_1g069725 24.876 402 257 10 47 444 103 463 4.27e-28 116
MS.gene043315.t1 MTR_4g077760 27.068 399 248 13 52 443 192 554 8.96e-26 110
MS.gene043315.t1 MTR_2g097380 25.000 396 225 11 60 446 231 563 3.30e-23 102
MS.gene043315.t1 MTR_7g074650 21.939 392 265 10 56 445 441 793 1.39e-22 101
MS.gene043315.t1 MTR_3g072710 28.571 322 188 11 134 446 214 502 2.22e-22 100
MS.gene043315.t1 MTR_8g093070 23.892 406 253 14 53 447 139 499 2.45e-19 90.9
MS.gene043315.t1 MTR_1g096030 21.718 419 260 12 53 443 80 458 8.92e-18 85.9
MS.gene043315.t1 MTR_7g057230 21.393 402 264 11 54 443 295 656 1.64e-15 79.3
MS.gene043315.t1 MTR_4g026485 23.573 403 246 14 54 443 270 623 3.29e-15 78.6
MS.gene043315.t1 MTR_5g058860 24.818 411 270 13 47 444 113 497 1.11e-14 76.6
MS.gene043315.t1 MTR_0092s0100 23.558 416 231 15 54 443 376 730 5.04e-14 74.7
MS.gene043315.t1 MTR_0092s0100 23.558 416 231 15 54 443 373 727 5.63e-14 74.7
MS.gene043315.t1 MTR_2g034250 20.414 338 227 8 54 379 211 518 5.99e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043315.t1 AT3G49950 43.891 442 205 11 13 445 2 409 7.20e-118 351
MS.gene043315.t1 AT4G37650 29.078 423 252 12 44 446 137 531 1.04e-51 183
MS.gene043315.t1 AT2G04890 27.960 397 243 9 53 443 53 412 5.00e-44 159
MS.gene043315.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene043315.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene043315.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene043315.t1 AT5G48150 28.535 396 248 8 54 443 123 489 1.34e-43 160
MS.gene043315.t1 AT1G21450 27.397 438 276 12 16 443 187 592 2.04e-41 155
MS.gene043315.t1 AT1G50600 27.182 401 254 8 50 443 156 525 3.88e-41 154
MS.gene043315.t1 AT1G50600 27.182 401 254 8 50 443 231 600 8.28e-41 154
MS.gene043315.t1 AT1G50600 27.182 401 254 8 50 443 227 596 1.19e-40 154
MS.gene043315.t1 AT1G50420 26.994 489 297 12 3 443 2 478 3.81e-39 147
MS.gene043315.t1 AT5G67411 39.631 217 106 8 171 381 1 198 2.33e-38 138
MS.gene043315.t1 AT1G07520 27.941 408 250 10 53 446 154 531 5.13e-36 140
MS.gene043315.t1 AT1G07520 27.941 408 250 10 53 446 154 531 5.13e-36 140
MS.gene043315.t1 AT1G07520 27.941 408 250 10 53 446 319 696 1.27e-35 140
MS.gene043315.t1 AT1G07520 27.941 408 250 10 53 446 318 695 1.41e-35 140
MS.gene043315.t1 AT2G29065 27.901 405 255 9 53 446 252 630 1.87e-35 139
MS.gene043315.t1 AT3G03450 26.119 402 258 10 47 443 177 544 3.43e-35 137
MS.gene043315.t1 AT4G17230 26.933 401 259 9 50 445 155 526 6.55e-35 137
MS.gene043315.t1 AT4G17230 26.933 401 259 9 50 445 155 526 6.55e-35 137
MS.gene043315.t1 AT1G07530 25.735 408 263 10 48 446 391 767 6.61e-35 138
MS.gene043315.t1 AT2G37650 26.290 407 253 11 50 445 344 714 2.64e-34 136
MS.gene043315.t1 AT2G37650 26.290 407 253 11 50 445 332 702 2.73e-34 136
MS.gene043315.t1 AT5G17490 26.585 410 253 10 47 449 154 522 6.16e-34 134
MS.gene043315.t1 AT2G01570 25.743 404 254 8 46 443 217 580 1.17e-32 130
MS.gene043315.t1 AT2G29060 25.792 442 279 12 18 445 250 656 3.22e-32 130
MS.gene043315.t1 AT2G29060 25.792 442 279 12 18 445 287 693 3.74e-32 130
MS.gene043315.t1 AT1G14920 26.119 402 255 11 46 443 165 528 4.07e-32 129
MS.gene043315.t1 AT1G63100 27.027 370 232 9 53 417 277 613 2.49e-31 127
MS.gene043315.t1 AT1G63100 27.027 370 232 9 53 417 252 588 2.61e-31 127
MS.gene043315.t1 AT1G66350 24.750 400 255 7 47 443 149 505 1.59e-30 124
MS.gene043315.t1 AT5G59450 25.672 409 262 12 50 447 224 601 1.35e-28 119
MS.gene043315.t1 AT3G46600 25.616 406 262 9 50 447 1 374 2.63e-28 115
MS.gene043315.t1 AT5G41920 24.631 406 259 11 46 443 33 399 2.77e-28 116
MS.gene043315.t1 AT3G46600 25.616 406 262 9 50 447 177 550 1.38e-27 115
MS.gene043315.t1 AT3G46600 25.616 406 262 9 50 447 209 582 2.39e-27 115
MS.gene043315.t1 AT5G66770 27.596 366 225 12 52 412 219 549 9.28e-24 104
MS.gene043315.t1 AT3G54220 23.704 405 265 11 46 445 286 651 1.41e-23 104
MS.gene043315.t1 AT3G13840 24.638 414 257 11 43 443 138 509 3.03e-23 102
MS.gene043315.t1 AT5G52510 22.359 407 273 8 49 443 264 639 3.42e-21 97.1
MS.gene043315.t1 AT1G55580 24.009 429 266 12 50 443 41 444 3.81e-19 90.1
MS.gene043315.t1 AT4G08250 25.648 386 246 12 68 444 124 477 1.88e-16 82.0
MS.gene043315.t1 AT3G50650 22.642 371 241 10 84 443 206 541 4.22e-15 78.2
MS.gene043315.t1 AT4G00150 24.485 437 258 18 18 443 178 553 4.07e-13 72.0

Find 96 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATAACGTAACCAATTT+TGG 0.129129 2.2:+10733604 None:intergenic
CCTGAACCAGAAGCCCTTAA+AGG 0.161852 2.2:-10734338 MS.gene043315:CDS
AGCTTCCAAGACAACCTTTA+AGG 0.184423 2.2:+10734324 None:intergenic
ATCCTAGCATGTTGAATCTT+AGG 0.231235 2.2:-10733653 MS.gene043315:CDS
TGGTTATAAATTGCCAAAAT+TGG 0.234975 2.2:-10733617 MS.gene043315:CDS
AAAACAGTTTCCTCACAAAA+TGG 0.244613 2.2:+10733240 None:intergenic
TAGGCCAATGGTTCCTCCTT+TGG 0.289757 2.2:-10733844 MS.gene043315:CDS
CCTTTAAGGGCTTCTGGTTC+AGG 0.298602 2.2:+10734338 None:intergenic
CAGGAAGAGGAGGAGTGTTT+TGG 0.299955 2.2:+10734357 None:intergenic
CATCAAAGGGTATCCATAAA+TGG 0.302600 2.2:+10733433 None:intergenic
AGACAACCTTTAAGGGCTTC+TGG 0.302693 2.2:+10734332 None:intergenic
GGTTGTCTTGGAAGCTTAGA+TGG 0.317852 2.2:-10734317 MS.gene043315:CDS
CTAAGTGAAGGAGGACCTTC+TGG 0.319718 2.2:+10733885 None:intergenic
GAAACCATGAAGTGAGTCTT+TGG 0.321815 2.2:+10734174 None:intergenic
CATGAAGTTGGTCAACGTTT+AGG 0.327762 2.2:-10733807 MS.gene043315:CDS
GTCCTTTGACAACTATCTTT+AGG 0.332169 2.2:+10733393 None:intergenic
CTAACCAAAGACTCACTTCA+TGG 0.347970 2.2:-10734178 MS.gene043315:CDS
TAACCCTCAAATAGTGCTAT+TGG 0.350512 2.2:-10733517 MS.gene043315:CDS
GCTTCCAAGACAACCTTTAA+GGG 0.359848 2.2:+10734325 None:intergenic
CTTGGGTCCCTAGTGAAATT+CGG 0.366646 2.2:-10733110 MS.gene043315:CDS
ATCAACATACCCGGCGAGTT+CGG 0.379075 2.2:+10734055 None:intergenic
CCAATAACATTAAACTCAAA+AGG 0.380341 2.2:+10733759 None:intergenic
AACAACGTTGCTTCACCATT+AGG 0.392639 2.2:-10734206 MS.gene043315:CDS
TTAATTCCTTGGCATAGGTT+TGG 0.394976 2.2:-10734032 MS.gene043315:CDS
ACTTCATTGTGCAAGTGCTT+TGG 0.396309 2.2:-10734273 MS.gene043315:CDS
GAGAATATTATAAGCTTTGA+AGG 0.397984 2.2:-10733339 MS.gene043315:CDS
TGACTTGAAAATTTCATTGT+TGG 0.405848 2.2:+10733998 None:intergenic
ATGGTGAAGCAACGTTGTTT+AGG 0.411496 2.2:+10734210 None:intergenic
AGGATAGAGAGATTGGAGTC+AGG 0.412485 2.2:-10733309 MS.gene043315:CDS
TCTTAGGGAAGATGAAGCTT+TGG 0.413076 2.2:-10733637 MS.gene043315:CDS
TTGGGTCCCTAGTGAAATTC+GGG 0.414052 2.2:-10733109 MS.gene043315:CDS
GCATCAAAGGATAGAGAGAT+TGG 0.414417 2.2:-10733316 MS.gene043315:CDS
CTTTGGTTAGTGTCACCTAA+TGG 0.430482 2.2:+10734191 None:intergenic
CTGCGTGTTTGCGACCGCTT+GGG 0.440188 2.2:-10733127 MS.gene043315:CDS
ATGGATACCCTTTGATGCAC+TGG 0.441346 2.2:-10733427 MS.gene043315:CDS
TGGTGTGTGTTGAAAAGTGT+TGG 0.441364 2.2:+10734377 None:intergenic
CATTAATCTCTAGAGCTTCA+AGG 0.442427 2.2:-10734145 MS.gene043315:CDS
GCTGCGTGTTTGCGACCGCT+TGG 0.447845 2.2:-10733128 MS.gene043315:CDS
AGGGCTTCTGGTTCAGGAAG+AGG 0.448419 2.2:+10734344 None:intergenic
TCCTAGCATGTTGAATCTTA+GGG 0.452667 2.2:-10733652 MS.gene043315:CDS
GGATAGAGAGATTGGAGTCA+GGG 0.452893 2.2:-10733308 MS.gene043315:CDS
TCAACCAATAGCACTATTTG+AGG 0.464072 2.2:+10733513 None:intergenic
GGTATGTTGATTTAATTCCT+TGG 0.469050 2.2:-10734043 MS.gene043315:CDS
GGAACCATTGGCCTAAAAGA+TGG 0.472154 2.2:+10733852 None:intergenic
TTATTTATCTGATGATAGAA+AGG 0.472508 2.2:-10733589 MS.gene043315:CDS
AGTCAATCAAGGGATTCAAA+AGG 0.473432 2.2:-10733980 MS.gene043315:CDS
AAAGTAGGCCATTGCATACA+AGG 0.474102 2.2:+10733930 None:intergenic
GAAGCCCTTAAAGGTTGTCT+TGG 0.476858 2.2:-10734329 MS.gene043315:CDS
AAAAGTGTCCAGTGCATCAA+AGG 0.477866 2.2:+10733419 None:intergenic
CCTTAGCTCAACAAGTTATG+TGG 0.481567 2.2:-10734235 MS.gene043315:CDS
GGTGTGTGTTGAAAAGTGTT+GGG 0.486954 2.2:+10734378 None:intergenic
AGTGAAATTCGGGATCATAT+CGG 0.488833 2.2:-10733099 MS.gene043315:CDS
CCTTTGAAATTCATTGAAGT+AGG 0.489707 2.2:+10734116 None:intergenic
AACATATCAATACATGAAGT+TGG 0.494100 2.2:-10733819 MS.gene043315:CDS
AAAGCATGTTGGTTCTTACA+TGG 0.498679 2.2:-10733161 MS.gene043315:CDS
CAATAACATTAAACTCAAAA+GGG 0.504289 2.2:+10733760 None:intergenic
GTCAATCAAGGGATTCAAAA+GGG 0.509034 2.2:-10733979 MS.gene043315:CDS
TTGATTTAATTCCTTGGCAT+AGG 0.515662 2.2:-10734037 MS.gene043315:CDS
CCTACTTCAATGAATTTCAA+AGG 0.532194 2.2:-10734116 MS.gene043315:CDS
CCACATAACTTGTTGAGCTA+AGG 0.533265 2.2:+10734235 None:intergenic
TATTTATCTGATGATAGAAA+GGG 0.534012 2.2:-10733588 MS.gene043315:CDS
GCCAAAGAATCTATAAAAGT+AGG 0.539244 2.2:+10733915 None:intergenic
ATGTCCGTTACCGAACTCGC+CGG 0.540316 2.2:-10734065 MS.gene043315:CDS
CAACCAATAGCACTATTTGA+GGG 0.543059 2.2:+10733514 None:intergenic
GAACCATTGGCCTAAAAGAT+GGG 0.544386 2.2:+10733853 None:intergenic
ATGTTGGTTCTTACATGGAA+AGG 0.544446 2.2:-10733156 MS.gene043315:CDS
TAAGCTTTGAAGGGCATCAA+AGG 0.546684 2.2:-10733329 MS.gene043315:CDS
GATGAACATGCTAGTGGATG+GGG 0.551613 2.2:-10733198 MS.gene043315:CDS
ATATGATCCCGAATTTCACT+AGG 0.561630 2.2:+10733102 None:intergenic
AGAATATTATAAGCTTTGAA+GGG 0.562232 2.2:-10733338 MS.gene043315:CDS
AGCTTTCATGTGAACGTGCA+TGG 0.563642 2.2:+10734436 None:intergenic
TGCCTAAAGATAGTTGTCAA+AGG 0.566343 2.2:-10733395 MS.gene043315:CDS
TTAACCAAAGGAGGAACCAT+TGG 0.568221 2.2:+10733840 None:intergenic
GTAACTACACAATTCAAAGA+AGG 0.569120 2.2:-10734091 MS.gene043315:CDS
TGTCCGTTACCGAACTCGCC+GGG 0.570400 2.2:-10734064 MS.gene043315:CDS
AATAGTGCTATTGGTTGATG+AGG 0.571491 2.2:-10733508 MS.gene043315:CDS
GCATAACACCTTGTATGCAA+TGG 0.571925 2.2:-10733938 MS.gene043315:CDS
AAAGTGTCCAGTGCATCAAA+GGG 0.575835 2.2:+10733420 None:intergenic
TCCCTAAGATTCAACATGCT+AGG 0.588496 2.2:+10733651 None:intergenic
TGTATTGATATGTTAACCAA+AGG 0.588615 2.2:+10733828 None:intergenic
ATTGATATGTTAACCAAAGG+AGG 0.601377 2.2:+10733831 None:intergenic
TTGATGAACATGCTAGTGGA+TGG 0.601862 2.2:-10733200 MS.gene043315:CDS
TGATGAACATGCTAGTGGAT+GGG 0.604800 2.2:-10733199 MS.gene043315:CDS
TCTTTGGCTAAATTACCAGA+AGG 0.605851 2.2:-10733900 MS.gene043315:CDS
TTGCTTGATGAACATGCTAG+TGG 0.606672 2.2:-10733204 MS.gene043315:CDS
GCTTCTGGTTCAGGAAGAGG+AGG 0.607512 2.2:+10734347 None:intergenic
CTTAGCTCAACAAGTTATGT+GGG 0.611843 2.2:-10734234 MS.gene043315:CDS
AGGAATTAAATCAACATACC+CGG 0.613372 2.2:+10734046 None:intergenic
GAAAAGAGAAGAAAGCATGT+TGG 0.614602 2.2:-10733172 MS.gene043315:CDS
ACAGAGTTTGAATCTGACAT+AGG 0.622989 2.2:-10733372 MS.gene043315:CDS
ATACCCGGCGAGTTCGGTAA+CGG 0.629291 2.2:+10734061 None:intergenic
GGGACTGTGATTCTAAGTGA+AGG 0.639863 2.2:+10733873 None:intergenic
TATGATCCCGAATTTCACTA+GGG 0.650286 2.2:+10733103 None:intergenic
ACTGTGATTCTAAGTGAAGG+AGG 0.660820 2.2:+10733876 None:intergenic
AATTCGGGATCATATCGGCA+TGG 0.665545 2.2:-10733094 MS.gene043315:CDS
AATTTCACTAGGGACCCAAG+CGG 0.769651 2.2:+10733113 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAACATTAAACTCAAAA+GGG + chr2.2:10733754-10733773 None:intergenic 20.0%
!! TATTTATCTGATGATAGAAA+GGG - chr2.2:10733923-10733942 MS.gene043315:CDS 20.0%
!! TTATTTATCTGATGATAGAA+AGG - chr2.2:10733922-10733941 MS.gene043315:CDS 20.0%
!!! AGAATATTATAAGCTTTGAA+GGG - chr2.2:10734173-10734192 MS.gene043315:CDS 20.0%
!!! AGGTAATTTTGCAAAATTTA+AGG - chr2.2:10733724-10733743 MS.gene043315:CDS 20.0%
!!! ATAAATAACGTAACCAATTT+TGG + chr2.2:10733910-10733929 None:intergenic 20.0%
!!! GCTTTTATGAATTTAGTTAA+AGG - chr2.2:10733968-10733987 MS.gene043315:CDS 20.0%
! AACATATCAATACATGAAGT+TGG - chr2.2:10733692-10733711 MS.gene043315:CDS 25.0%
! CCAATAACATTAAACTCAAA+AGG + chr2.2:10733755-10733774 None:intergenic 25.0%
! TGACTTGAAAATTTCATTGT+TGG + chr2.2:10733516-10733535 None:intergenic 25.0%
! TGGTTATAAATTGCCAAAAT+TGG - chr2.2:10733894-10733913 MS.gene043315:CDS 25.0%
!! GAGAATATTATAAGCTTTGA+AGG - chr2.2:10734172-10734191 MS.gene043315:CDS 25.0%
!! TGTATTGATATGTTAACCAA+AGG + chr2.2:10733686-10733705 None:intergenic 25.0%
!!! CCTTTTGAGTTTAATGTTAT+TGG - chr2.2:10733752-10733771 MS.gene043315:CDS 25.0%
!!! GAAATTTTCAAGTCAATCAA+GGG - chr2.2:10733521-10733540 MS.gene043315:CDS 25.0%
!!! TGAAATTTTCAAGTCAATCA+AGG - chr2.2:10733520-10733539 MS.gene043315:CDS 25.0%
AAAACAGTTTCCTCACAAAA+TGG + chr2.2:10734274-10734293 None:intergenic 30.0%
AGGAATTAAATCAACATACC+CGG + chr2.2:10733468-10733487 None:intergenic 30.0%
ATTGATATGTTAACCAAAGG+AGG + chr2.2:10733683-10733702 None:intergenic 30.0%
CCTACTTCAATGAATTTCAA+AGG - chr2.2:10733395-10733414 MS.gene043315:CDS 30.0%
GCCAAAGAATCTATAAAAGT+AGG + chr2.2:10733599-10733618 None:intergenic 30.0%
GTAACTACACAATTCAAAGA+AGG - chr2.2:10733420-10733439 MS.gene043315:CDS 30.0%
TCACAAAGAATGAAAAATGC+TGG - chr2.2:10734235-10734254 MS.gene043315:CDS 30.0%
TTGATTTAATTCCTTGGCAT+AGG - chr2.2:10733474-10733493 MS.gene043315:CDS 30.0%
! ATTTTGTGAGGAAACTGTTT+TGG - chr2.2:10734273-10734292 MS.gene043315:CDS 30.0%
! CTGCTTCTTTTAACCATTTA+TGG - chr2.2:10734065-10734084 MS.gene043315:CDS 30.0%
!! CCTTTGAAATTCATTGAAGT+AGG + chr2.2:10733398-10733417 None:intergenic 30.0%
!! GCCTACTTTTATAGATTCTT+TGG - chr2.2:10733595-10733614 MS.gene043315:CDS 30.0%
!! GGTATGTTGATTTAATTCCT+TGG - chr2.2:10733468-10733487 MS.gene043315:CDS 30.0%
ACAGAGTTTGAATCTGACAT+AGG - chr2.2:10734139-10734158 MS.gene043315:CDS 35.0%
AGTCAATCAAGGGATTCAAA+AGG - chr2.2:10733531-10733550 MS.gene043315:CDS 35.0%
AGTGAAATTCGGGATCATAT+CGG - chr2.2:10734412-10734431 MS.gene043315:CDS 35.0%
ATATGATCCCGAATTTCACT+AGG + chr2.2:10734412-10734431 None:intergenic 35.0%
ATCCTAGCATGTTGAATCTT+AGG - chr2.2:10733858-10733877 MS.gene043315:CDS 35.0%
CAACCAATAGCACTATTTGA+GGG + chr2.2:10734000-10734019 None:intergenic 35.0%
CTTAGCTCAACAAGTTATGT+GGG - chr2.2:10733277-10733296 MS.gene043315:CDS 35.0%
GTCAATCAAGGGATTCAAAA+GGG - chr2.2:10733532-10733551 MS.gene043315:CDS 35.0%
GTCCTTTGACAACTATCTTT+AGG + chr2.2:10734121-10734140 None:intergenic 35.0%
TAACCCTCAAATAGTGCTAT+TGG - chr2.2:10733994-10734013 MS.gene043315:CDS 35.0%
TATGATCCCGAATTTCACTA+GGG + chr2.2:10734411-10734430 None:intergenic 35.0%
TCAACCAATAGCACTATTTG+AGG + chr2.2:10734001-10734020 None:intergenic 35.0%
TCCTAGCATGTTGAATCTTA+GGG - chr2.2:10733859-10733878 MS.gene043315:CDS 35.0%
TCTTTGGCTAAATTACCAGA+AGG - chr2.2:10733611-10733630 MS.gene043315:CDS 35.0%
TGCCTAAAGATAGTTGTCAA+AGG - chr2.2:10734116-10734135 MS.gene043315:CDS 35.0%
TTAATTCCTTGGCATAGGTT+TGG - chr2.2:10733479-10733498 MS.gene043315:CDS 35.0%
! AATAGTGCTATTGGTTGATG+AGG - chr2.2:10734003-10734022 MS.gene043315:CDS 35.0%
! CTTTAGTGTTCCATTTTGTG+AGG - chr2.2:10734261-10734280 MS.gene043315:CDS 35.0%
! GAAAAGAGAAGAAAGCATGT+TGG - chr2.2:10734339-10734358 MS.gene043315:CDS 35.0%
!! AAAGCATGTTGGTTCTTACA+TGG - chr2.2:10734350-10734369 MS.gene043315:CDS 35.0%
!! ATGTTGGTTCTTACATGGAA+AGG - chr2.2:10734355-10734374 MS.gene043315:CDS 35.0%
!! CATCAAAGGGTATCCATAAA+TGG + chr2.2:10734081-10734100 None:intergenic 35.0%
!! CATTAATCTCTAGAGCTTCA+AGG - chr2.2:10733366-10733385 MS.gene043315:CDS 35.0%
!!! GAAAAGTGTTGGGTTTTGAA+AGG + chr2.2:10733126-10733145 None:intergenic 35.0%
!!! GAAACTGTTTTGGAAGTCAA+GGG - chr2.2:10734283-10734302 MS.gene043315:CDS 35.0%
AAAGTAGGCCATTGCATACA+AGG + chr2.2:10733584-10733603 None:intergenic 40.0%
AACAACGTTGCTTCACCATT+AGG - chr2.2:10733305-10733324 MS.gene043315:CDS 40.0%
ACTGTGATTCTAAGTGAAGG+AGG + chr2.2:10733638-10733657 None:intergenic 40.0%
ACTTCATTGTGCAAGTGCTT+TGG - chr2.2:10733238-10733257 MS.gene043315:CDS 40.0%
AGCTTCCAAGACAACCTTTA+AGG + chr2.2:10733190-10733209 None:intergenic 40.0%
ATGGTGAAGCAACGTTGTTT+AGG + chr2.2:10733304-10733323 None:intergenic 40.0%
CAAAAACCAAACCTATGCCA+AGG + chr2.2:10733488-10733507 None:intergenic 40.0%
CCTTAGCTCAACAAGTTATG+TGG - chr2.2:10733276-10733295 MS.gene043315:CDS 40.0%
CTAACCAAAGACTCACTTCA+TGG - chr2.2:10733333-10733352 MS.gene043315:CDS 40.0%
CTTTGGTTAGTGTCACCTAA+TGG + chr2.2:10733323-10733342 None:intergenic 40.0%
GAAACCATGAAGTGAGTCTT+TGG + chr2.2:10733340-10733359 None:intergenic 40.0%
GCATAACACCTTGTATGCAA+TGG - chr2.2:10733573-10733592 MS.gene043315:CDS 40.0%
GCATCAAAGGATAGAGAGAT+TGG - chr2.2:10734195-10734214 MS.gene043315:CDS 40.0%
GCTTCCAAGACAACCTTTAA+GGG + chr2.2:10733189-10733208 None:intergenic 40.0%
TCCCTAAGATTCAACATGCT+AGG + chr2.2:10733863-10733882 None:intergenic 40.0%
TCTTAGGGAAGATGAAGCTT+TGG - chr2.2:10733874-10733893 MS.gene043315:CDS 40.0%
TTAACCAAAGGAGGAACCAT+TGG + chr2.2:10733674-10733693 None:intergenic 40.0%
TTGATGAACATGCTAGTGGA+TGG - chr2.2:10734311-10734330 MS.gene043315:CDS 40.0%
TTGCTTGATGAACATGCTAG+TGG - chr2.2:10734307-10734326 MS.gene043315:CDS 40.0%
! AAAGTGTCCAGTGCATCAAA+GGG + chr2.2:10734094-10734113 None:intergenic 40.0%
! CATGAAGTTGGTCAACGTTT+AGG - chr2.2:10733704-10733723 MS.gene043315:CDS 40.0%
! GAACCATTGGCCTAAAAGAT+GGG + chr2.2:10733661-10733680 None:intergenic 40.0%
! GAATCACAGTCCCATCTTTT+AGG - chr2.2:10733648-10733667 MS.gene043315:CDS 40.0%
! TGATGAACATGCTAGTGGAT+GGG - chr2.2:10734312-10734331 MS.gene043315:CDS 40.0%
! TGGTGTGTGTTGAAAAGTGT+TGG + chr2.2:10733137-10733156 None:intergenic 40.0%
!! AAAAGTGTCCAGTGCATCAA+AGG + chr2.2:10734095-10734114 None:intergenic 40.0%
!! CCACATAACTTGTTGAGCTA+AGG + chr2.2:10733279-10733298 None:intergenic 40.0%
!! GGTGTGTGTTGAAAAGTGTT+GGG + chr2.2:10733136-10733155 None:intergenic 40.0%
!! TAAGCTTTGAAGGGCATCAA+AGG - chr2.2:10734182-10734201 MS.gene043315:CDS 40.0%
!!! GGAAACTGTTTTGGAAGTCA+AGG - chr2.2:10734282-10734301 MS.gene043315:CDS 40.0%
AATTCGGGATCATATCGGCA+TGG - chr2.2:10734417-10734436 MS.gene043315:CDS 45.0%
AATTTCACTAGGGACCCAAG+CGG + chr2.2:10734401-10734420 None:intergenic 45.0%
AGGATAGAGAGATTGGAGTC+AGG - chr2.2:10734202-10734221 MS.gene043315:CDS 45.0%
ATGGATACCCTTTGATGCAC+TGG - chr2.2:10734084-10734103 MS.gene043315:CDS 45.0%
CTTGGGTCCCTAGTGAAATT+CGG - chr2.2:10734401-10734420 MS.gene043315:CDS 45.0%
GAAGCCCTTAAAGGTTGTCT+TGG - chr2.2:10733182-10733201 MS.gene043315:CDS 45.0%
GGATAGAGAGATTGGAGTCA+GGG - chr2.2:10734203-10734222 MS.gene043315:CDS 45.0%
GGGACTGTGATTCTAAGTGA+AGG + chr2.2:10733641-10733660 None:intergenic 45.0%
GGTTGTCTTGGAAGCTTAGA+TGG - chr2.2:10733194-10733213 MS.gene043315:CDS 45.0%
TTGGGTCCCTAGTGAAATTC+GGG - chr2.2:10734402-10734421 MS.gene043315:CDS 45.0%
! AGACAACCTTTAAGGGCTTC+TGG + chr2.2:10733182-10733201 None:intergenic 45.0%
! AGTCCCATCTTTTAGGCCAA+TGG - chr2.2:10733655-10733674 MS.gene043315:CDS 45.0%
! GATGAACATGCTAGTGGATG+GGG - chr2.2:10734313-10734332 MS.gene043315:CDS 45.0%
! GGAACCATTGGCCTAAAAGA+TGG + chr2.2:10733662-10733681 None:intergenic 45.0%
ATCAACATACCCGGCGAGTT+CGG + chr2.2:10733459-10733478 None:intergenic 50.0%
CCTGAACCAGAAGCCCTTAA+AGG - chr2.2:10733173-10733192 MS.gene043315:CDS 50.0%
CTAAGTGAAGGAGGACCTTC+TGG + chr2.2:10733629-10733648 None:intergenic 50.0%
TAGGCCAATGGTTCCTCCTT+TGG - chr2.2:10733667-10733686 MS.gene043315:CDS 50.0%
!! CCTTTAAGGGCTTCTGGTTC+AGG + chr2.2:10733176-10733195 None:intergenic 50.0%
!!! CAGGAAGAGGAGGAGTGTTT+TGG + chr2.2:10733157-10733176 None:intergenic 50.0%
ATACCCGGCGAGTTCGGTAA+CGG + chr2.2:10733453-10733472 None:intergenic 55.0%
ATGTCCGTTACCGAACTCGC+CGG - chr2.2:10733446-10733465 MS.gene043315:CDS 55.0%
!! AGGGCTTCTGGTTCAGGAAG+AGG + chr2.2:10733170-10733189 None:intergenic 55.0%
!! GCTTCTGGTTCAGGAAGAGG+AGG + chr2.2:10733167-10733186 None:intergenic 55.0%
CTGCGTGTTTGCGACCGCTT+GGG - chr2.2:10734384-10734403 MS.gene043315:CDS 60.0%
TGTCCGTTACCGAACTCGCC+GGG - chr2.2:10733447-10733466 MS.gene043315:CDS 60.0%
GCTGCGTGTTTGCGACCGCT+TGG - chr2.2:10734383-10734402 MS.gene043315:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 10733077 10734456 10733077 ID=MS.gene043315
chr2.2 mRNA 10733077 10734456 10733077 ID=MS.gene043315.t1;Parent=MS.gene043315
chr2.2 exon 10733077 10734456 10733077 ID=MS.gene043315.t1.exon1;Parent=MS.gene043315.t1
chr2.2 CDS 10733077 10734456 10733077 ID=cds.MS.gene043315.t1;Parent=MS.gene043315.t1
Gene Sequence

>MS.gene043315

ATGCACGTTCACATGAAAGCTGAGTTAAAAGCAGCAACATCTATTTCCTTTCAAAACCCAACACTTTTCAACACACACCAAAACACTCCTCCTCTTCCTGAACCAGAAGCCCTTAAAGGTTGTCTTGGAAGCTTAGATGGAGCATGTATTGAAAAGCTCTTACTTCATTGTGCAAGTGCTTTGGAAAGCAATGATATAACCTTAGCTCAACAAGTTATGTGGGTCCTAAACAACGTTGCTTCACCATTAGGTGACACTAACCAAAGACTCACTTCATGGTTTCTAAGAGCATTAATCTCTAGAGCTTCAAGGATTTGTCCTACTTCAATGAATTTCAAAGGAAGTAACTACACAATTCAAAGAAGGTTGATGTCCGTTACCGAACTCGCCGGGTATGTTGATTTAATTCCTTGGCATAGGTTTGGTTTTTGTGCTTCCAACAATGAAATTTTCAAGTCAATCAAGGGATTCAAAAGGGTACATATTTTAGATTTTAGCATAACACCTTGTATGCAATGGCCTACTTTTATAGATTCTTTGGCTAAATTACCAGAAGGTCCTCCTTCACTTAGAATCACAGTCCCATCTTTTAGGCCAATGGTTCCTCCTTTGGTTAACATATCAATACATGAAGTTGGTCAACGTTTAGGTAATTTTGCAAAATTTAAGGATGTCCCTTTTGAGTTTAATGTTATTGGAGATAATGTTTCATTGACTTCTGAAGATTTGAGCAATATTGAATCAACAAATTTTCATTTTGAATCAATGTTGAGTCTCTTGAATCCTAGCATGTTGAATCTTAGGGAAGATGAAGCTTTGGTTATAAATTGCCAAAATTGGTTACGTTATTTATCTGATGATAGAAAGGGTCAAAATATTTCACTTAGAGATGCTTTTATGAATTTAGTTAAAGGACTTAACCCTCAAATAGTGCTATTGGTTGATGAGGATTGTGATCTTAGTGCATCAAGTTTAACATCAAGAATTACTGCTTCTTTTAACCATTTATGGATACCCTTTGATGCACTGGACACTTTTTTGCCTAAAGATAGTTGTCAAAGGACAGAGTTTGAATCTGACATAGGACAAAAAATTGAGAATATTATAAGCTTTGAAGGGCATCAAAGGATAGAGAGATTGGAGTCAGGGATGCAAATGTCACAAAGAATGAAAAATGCTGGTTACTTTAGTGTTCCATTTTGTGAGGAAACTGTTTTGGAAGTCAAGGGTTTGCTTGATGAACATGCTAGTGGATGGGGAATGAAAAGAGAAGAAAGCATGTTGGTTCTTACATGGAAAGGAAATAGCTGCGTGTTTGCGACCGCTTGGGTCCCTAGTGAAATTCGGGATCATATCGGCATGGATGCACGTGTGTCGTAG
Protein sequence

>MS.gene043315.t1

MHVHMKAELKAATSISFQNPTLFNTHQNTPPLPEPEALKGCLGSLDGACIEKLLLHCASALESNDITLAQQVMWVLNNVASPLGDTNQRLTSWFLRALISRASRICPTSMNFKGSNYTIQRRLMSVTELAGYVDLIPWHRFGFCASNNEIFKSIKGFKRVHILDFSITPCMQWPTFIDSLAKLPEGPPSLRITVPSFRPMVPPLVNISIHEVGQRLGNFAKFKDVPFEFNVIGDNVSLTSEDLSNIESTNFHFESMLSLLNPSMLNLREDEALVINCQNWLRYLSDDRKGQNISLRDAFMNLVKGLNPQIVLLVDEDCDLSASSLTSRITASFNHLWIPFDALDTFLPKDSCQRTEFESDIGQKIENIISFEGHQRIERLESGMQMSQRMKNAGYFSVPFCEETVLEVKGLLDEHASGWGMKREESMLVLTWKGNSCVFATAWVPSEIRDHIGMDARVS