AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043425

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043425.t1 MTR_2g090480 87.192 203 22 2 1 200 1 202 3.06e-126 357
MS.gene043425.t1 MTR_2g090425 83.092 207 32 1 1 204 1 207 9.89e-121 347
MS.gene043425.t1 MTR_2g090440 82.500 200 35 0 1 200 1 200 6.08e-120 340
MS.gene043425.t1 MTR_2g090500 81.818 154 28 0 47 200 60 213 4.60e-87 258
MS.gene043425.t1 MTR_4g078350 64.824 199 55 5 9 199 8 199 1.32e-84 251
MS.gene043425.t1 MTR_2g013920 43.023 172 91 3 12 181 6 172 2.19e-42 142
MS.gene043425.t1 MTR_2g090515 67.857 56 17 1 1 56 1 55 9.68e-18 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043425.t1 AT4G20970 38.547 179 96 5 13 180 11 186 5.67e-31 113
MS.gene043425.t1 AT1G10585 29.609 179 108 5 11 184 12 177 3.58e-15 71.6
MS.gene043425.t1 AT1G10586 25.824 182 124 5 1 180 1 173 2.93e-11 60.8

Find 60 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTCTGGGATCTTGAAAT+TGG 0.192948 2.2:+9235284 MS.gene043425:CDS
TTTCAATCTCTCGCCTATTT+TGG 0.215651 2.2:-9235219 None:intergenic
TATCTCTTCAAATTCTTCTT+TGG 0.234992 2.2:+9233537 MS.gene043425:CDS
TGGAAACACAACCTCAATTC+TGG 0.245971 2.2:+9235270 MS.gene043425:CDS
GGTGGCTGCTCGAGTTCTTC+TGG 0.260572 2.2:+9233382 MS.gene043425:CDS
AGAGAAGAAAGAAAGTTTAA+TGG 0.275748 2.2:+9233339 MS.gene043425:CDS
CCGCAATCTTTGTTACGATT+TGG 0.283657 2.2:+9235191 MS.gene043425:CDS
ATCACCTTTGGATCATAGTT+AGG 0.324173 2.2:-9232814 None:intergenic
CTCTCCCAGCTTCACCGTTC+AGG 0.324702 2.2:-9236401 None:intergenic
GAGAGTTGAGTTTAGAAAAG+AGG 0.353043 2.2:-9232786 None:intergenic
GAGAAGAAAGAAAGTTTAAT+GGG 0.355968 2.2:+9233340 MS.gene043425:CDS
CGATTTGGAGAAACCAAAAT+AGG 0.365801 2.2:+9235206 MS.gene043425:CDS
TAGAAAAGAGGATCTTCATT+TGG 0.386590 2.2:-9232774 None:intergenic
TTCGACCTGAACGGTGAAGC+TGG 0.395736 2.2:+9236396 MS.gene043425:CDS
ATTGCCTCATCTACTTGATC+AGG 0.400525 2.2:-9233274 None:intergenic
TCTTCAAATTCTTCTTTGGC+TGG 0.403407 2.2:+9233541 MS.gene043425:CDS
TAACTTCACAGAAAATAAAA+TGG 0.408004 2.2:-9233483 None:intergenic
AACGATCTTTCTTTCTACTT+TGG 0.409199 2.2:-9232734 None:intergenic
CAAACATTATATTTGTCTCT+AGG 0.410779 2.2:-9233312 None:intergenic
CGAGAGATTGAAAGCATTTG+TGG 0.420058 2.2:+9235229 MS.gene043425:CDS
TTTGAGCTAGTCAAGCTAAA+GGG 0.452153 2.2:+9236369 MS.gene043425:CDS
AAAATAGAGATTCATGAAAT+GGG 0.456591 2.2:+9233421 MS.gene043425:CDS
TCTACTTTGGTTGAAGAAGA+AGG 0.464564 2.2:-9232721 None:intergenic
TCACCTTTGGATCATAGTTA+GGG 0.469862 2.2:-9232813 None:intergenic
ATTTCAAGATCCCAGAATTG+AGG 0.481933 2.2:-9235281 None:intergenic
GGAAACACAACCTCAATTCT+GGG 0.497186 2.2:+9235271 MS.gene043425:CDS
TGCTGAGCTAGGCATGTTGA+AGG 0.508960 2.2:+9236287 MS.gene043425:intron
TCATCTACTTGATCAGGTAA+TGG 0.516045 2.2:-9233268 None:intergenic
TCTTCATACCGTACATGCTC+AGG 0.517547 2.2:+9233573 MS.gene043425:CDS
AGCTAAGCTAGACATGTTGA+AGG 0.522464 2.2:+9236341 MS.gene043425:CDS
TTGTGTTTCCACATTGCTCA+CGG 0.538142 2.2:-9235258 None:intergenic
TATATATACCTGAGCATGTA+CGG 0.538419 2.2:-9233581 None:intergenic
ATTTGAGCTAGTCAAGCTAA+AGG 0.543971 2.2:+9236368 MS.gene043425:CDS
TACAAGTCATTGTAACATGT+GGG 0.544345 2.2:+9233452 MS.gene043425:CDS
TTGAAGGGTGGAGCTGAGCT+AGG 0.553003 2.2:+9236303 MS.gene043425:CDS
TCGACCTGAACGGTGAAGCT+GGG 0.558540 2.2:+9236397 MS.gene043425:CDS
AGATTGAAAGCATTTGTGGA+AGG 0.584279 2.2:+9235233 MS.gene043425:CDS
CTACAAGTCATTGTAACATG+TGG 0.589705 2.2:+9233451 MS.gene043425:CDS
TCTCCCTAACTATGATCCAA+AGG 0.591025 2.2:+9232810 MS.gene043425:CDS
TTGAGCTAGTCAAGCTAAAG+GGG 0.591345 2.2:+9236370 MS.gene043425:CDS
CCAAATCGTAACAAAGATTG+CGG 0.605051 2.2:-9235191 None:intergenic
AAGCTGGGAGAGCATGGTGA+AGG 0.612141 2.2:+9236412 MS.gene043425:CDS
AGCTGAGCTAGGTATGCTGA+AGG 0.624744 2.2:+9236314 MS.gene043425:CDS
AATAAGAGATCACGTGATGG+TGG 0.625950 2.2:+9233364 MS.gene043425:CDS
TGTTACAATGACTTGTAGAG+TGG 0.628096 2.2:-9233446 None:intergenic
ATTACCTGATCAAGTAGATG+AGG 0.635272 2.2:+9233270 MS.gene043425:CDS
AAGCTAGACATGTTGAAGGG+TGG 0.644479 2.2:+9236345 MS.gene043425:CDS
ACGGTGAAGCTGGGAGAGCA+TGG 0.655402 2.2:+9236406 MS.gene043425:CDS
GCTAAGCTAGACATGTTGAA+GGG 0.660132 2.2:+9236342 MS.gene043425:CDS
GAGCTAGGTATGCTGAAGGG+TGG 0.668247 2.2:+9236318 MS.gene043425:CDS
GGAAATAAGAGATCACGTGA+TGG 0.672227 2.2:+9233361 MS.gene043425:CDS
GAGCTAGGCATGTTGAAGGG+TGG 0.679340 2.2:+9236291 MS.gene043425:intron
ACAAGTCATTGTAACATGTG+GGG 0.680252 2.2:+9233453 MS.gene043425:CDS
GCTGAGCTAGGCATGTTGAA+GGG 0.691751 2.2:+9236288 MS.gene043425:intron
TAAATGGATCAAAATCAACA+AGG 0.698469 2.2:+9232691 None:intergenic
GCTGAGCTAGGTATGCTGAA+GGG 0.699239 2.2:+9236315 MS.gene043425:CDS
ATGGATCAAAATCAACAAGG+AGG 0.707629 2.2:+9232694 MS.gene043425:CDS
TGAGCTAGTCAAGCTAAAGG+GGG 0.709279 2.2:+9236371 MS.gene043425:CDS
ACTGCATGAAGAAAACGTCG+AGG 0.726422 2.2:+9233513 MS.gene043425:CDS
GGAAGGATCCGTGAGCAATG+TGG 0.734055 2.2:+9235250 MS.gene043425:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATTTTTAATAAAATTT+TGG + chr2.2:9235515-9235534 MS.gene043425:intron 0.0%
!!! TATTTTATTTTATAAGCTAA+GGG + chr2.2:9232842-9232861 MS.gene043425:intron 10.0%
!!! TTATTGCAATAAATTTTATA+CGG - chr2.2:9234126-9234145 None:intergenic 10.0%
!!! TTATTTTATTTTATAAGCTA+AGG + chr2.2:9232841-9232860 MS.gene043425:intron 10.0%
!! AAATCCAAAAACTTAAAAAA+TGG + chr2.2:9234861-9234880 MS.gene043425:intron 15.0%
!! CTTGTCAAAAAAAAAAAAAA+AGG + chr2.2:9234558-9234577 MS.gene043425:intron 15.0%
!! TAAAATAAAAAAATCACCTT+TGG - chr2.2:9232829-9232848 None:intergenic 15.0%
!! TGTTAATTAATTAAAAGAAG+AGG - chr2.2:9233162-9233181 None:intergenic 15.0%
!! TTGAATAACACATATTAATT+TGG + chr2.2:9233211-9233230 MS.gene043425:intron 15.0%
!! TTTCAATGTAAGAATAAAAT+TGG + chr2.2:9235721-9235740 MS.gene043425:intron 15.0%
!!! ATTTCCATTTTTTAAGTTTT+TGG - chr2.2:9234868-9234887 None:intergenic 15.0%
!!! TATCAATTGTTTCTATTTTA+GGG + chr2.2:9233865-9233884 MS.gene043425:intron 15.0%
!!! TTATCAATTGTTTCTATTTT+AGG + chr2.2:9233864-9233883 MS.gene043425:intron 15.0%
!!! TTGTTATTGCAATAAATTTT+AGG - chr2.2:9234069-9234088 None:intergenic 15.0%
!! AAAAATAGAGATTCATGAAA+TGG + chr2.2:9233420-9233439 MS.gene043425:CDS 20.0%
!! AAAATAGAGATTCATGAAAT+GGG + chr2.2:9233421-9233440 MS.gene043425:CDS 20.0%
!! AAATGACATGTCATAAAAAA+TGG - chr2.2:9234664-9234683 None:intergenic 20.0%
!! AAGAAAAAAATGTTAGATCA+TGG - chr2.2:9234744-9234763 None:intergenic 20.0%
!! AATGACATGTCATAAAAAAT+GGG - chr2.2:9234663-9234682 None:intergenic 20.0%
!! AATTCTGAATATATTCTTGT+AGG - chr2.2:9235634-9235653 None:intergenic 20.0%
!! ATTCTGAATATATTCTTGTA+GGG - chr2.2:9235633-9235652 None:intergenic 20.0%
!! TAACTTCACAGAAAATAAAA+TGG - chr2.2:9233486-9233505 None:intergenic 20.0%
!! TATATATATACATACACACA+TGG + chr2.2:9233596-9233615 MS.gene043425:intron 20.0%
!! TATTTCTTAACTTATGTCTA+AGG + chr2.2:9234621-9234640 MS.gene043425:intron 20.0%
!!! AAATGATTGATTCATAATCA+AGG + chr2.2:9235482-9235501 MS.gene043425:intron 20.0%
!!! ATTTCATGAATCTCTATTTT+TGG - chr2.2:9233421-9233440 None:intergenic 20.0%
!!! CAAAAGTTTTTTTTTTGTGA+CGG - chr2.2:9235686-9235705 None:intergenic 20.0%
!!! CAATTTTGAAAATTTAGTGT+AGG + chr2.2:9234343-9234362 MS.gene043425:intron 20.0%
!!! GTCGATTTTTTTTTTTTTAC+AGG - chr2.2:9232939-9232958 None:intergenic 20.0%
!!! TGTTATTTTTCATTTAATGC+AGG + chr2.2:9233234-9233253 MS.gene043425:intron 20.0%
!!! TTATTGCAATAAATTTTAGG+AGG - chr2.2:9234066-9234085 None:intergenic 20.0%
!!! TTTTGTAAACACCTTTTATT+TGG - chr2.2:9233047-9233066 None:intergenic 20.0%
! AAATGTTAGATCATGGTTTA+TGG - chr2.2:9234737-9234756 None:intergenic 25.0%
! ACACTCTAATAAGTGTAAAA+TGG - chr2.2:9233097-9233116 None:intergenic 25.0%
! ACATATATGCTATACATACA+TGG - chr2.2:9235599-9235618 None:intergenic 25.0%
! ACATATCACTATTGTTCTTT+GGG + chr2.2:9233724-9233743 MS.gene043425:intron 25.0%
! AGAGAAGAAAGAAAGTTTAA+TGG + chr2.2:9233339-9233358 MS.gene043425:CDS 25.0%
! ATCTAGTTACTTATATAGAG+AGG - chr2.2:9233990-9234009 None:intergenic 25.0%
! CAAACATTATATTTGTCTCT+AGG - chr2.2:9233315-9233334 None:intergenic 25.0%
! CAATTGTCACATATGTTTAA+CGG + chr2.2:9233018-9233037 MS.gene043425:intron 25.0%
! CGATTTAATCTCATATATGA+TGG - chr2.2:9234222-9234241 None:intergenic 25.0%
! CTTTAATCTATTCTTCATCA+AGG - chr2.2:9235362-9235381 None:intergenic 25.0%
! GAATATACATATGAATGTGA+TGG - chr2.2:9232883-9232902 None:intergenic 25.0%
! GAGAAGAAAGAAAGTTTAAT+GGG + chr2.2:9233340-9233359 MS.gene043425:CDS 25.0%
! GAGTACCTATATATTCATAT+AGG + chr2.2:9233132-9233151 MS.gene043425:intron 25.0%
! GTGAACCTATATGAATATAT+AGG - chr2.2:9233140-9233159 None:intergenic 25.0%
! TACATATCACTATTGTTCTT+TGG + chr2.2:9233723-9233742 MS.gene043425:intron 25.0%
! TATAAAAGATGACACTTAGA+AGG - chr2.2:9235881-9235900 None:intergenic 25.0%
! TATCTCTTCAAATTCTTCTT+TGG + chr2.2:9233537-9233556 MS.gene043425:CDS 25.0%
! TCTAGTTACTTATATAGAGA+GGG - chr2.2:9233989-9234008 None:intergenic 25.0%
! TTCATATGTATATTCATGTG+AGG + chr2.2:9232888-9232907 MS.gene043425:intron 25.0%
! TTCTGAATATATTCTTGTAG+GGG - chr2.2:9235632-9235651 None:intergenic 25.0%
! TTTAACGGTTTCCAAATAAA+AGG + chr2.2:9233033-9233052 MS.gene043425:intron 25.0%
!! AATAAATTTTATACGGTAGC+AGG - chr2.2:9234119-9234138 None:intergenic 25.0%
!! CTCTAAAAAGCTAGAATAAA+TGG + chr2.2:9235844-9235863 MS.gene043425:intron 25.0%
!! GAAGAATAGATTAAAGTATC+TGG + chr2.2:9235366-9235385 MS.gene043425:intron 25.0%
!! GTCTTAACAATATTTTGTGA+AGG + chr2.2:9235088-9235107 MS.gene043425:intron 25.0%
!! GTTAGTATGTGTTTTTCTAA+AGG - chr2.2:9234434-9234453 None:intergenic 25.0%
!! GTTATTCAATGAAGCTTTTT+AGG + chr2.2:9235805-9235824 MS.gene043425:intron 25.0%
!!! AATTTTAGGAGGAAAAAATC+AGG - chr2.2:9234055-9234074 None:intergenic 25.0%
!!! CTAATATTTTTGGTACCTTT+GGG + chr2.2:9235396-9235415 MS.gene043425:intron 25.0%
!!! TAATATTTTTGGTACCTTTG+GGG + chr2.2:9235397-9235416 MS.gene043425:intron 25.0%
!!! TCTAATATTTTTGGTACCTT+TGG + chr2.2:9235395-9235414 MS.gene043425:intron 25.0%
!!! TGGATCGATTCTAATATTTT+TGG + chr2.2:9235386-9235405 MS.gene043425:intron 25.0%
AACAAATAACCTCCATGAAA+GGG + chr2.2:9234144-9234163 MS.gene043425:intron 30.0%
AACGATCTTTCTTTCTACTT+TGG - chr2.2:9232737-9232756 None:intergenic 30.0%
AGCCAAAAAACTTGAATAGT+GGG + chr2.2:9233640-9233659 MS.gene043425:intron 30.0%
ATATATAGGTACTCTACTTG+TGG - chr2.2:9233126-9233145 None:intergenic 30.0%
CATCTAAAAAGAGTTCCATT+AGG - chr2.2:9235920-9235939 None:intergenic 30.0%
TAACAAATAACCTCCATGAA+AGG + chr2.2:9234143-9234162 MS.gene043425:intron 30.0%
TAATTGCAGTTCCTTCAATA+CGG + chr2.2:9234090-9234109 MS.gene043425:intron 30.0%
TACAAGTCATTGTAACATGT+GGG + chr2.2:9233452-9233471 MS.gene043425:CDS 30.0%
TAGAAAAGAGGATCTTCATT+TGG - chr2.2:9232777-9232796 None:intergenic 30.0%
TATATATACCTGAGCATGTA+CGG - chr2.2:9233584-9233603 None:intergenic 30.0%
TCATCACTTTATTGACCTAA+TGG + chr2.2:9235902-9235921 MS.gene043425:intron 30.0%
TCTATTCTTCATCAAGGATT+AGG - chr2.2:9235356-9235375 None:intergenic 30.0%
TTAACACTATAAGCTCTTCT+TGG + chr2.2:9233187-9233206 MS.gene043425:intron 30.0%
! TTGATGATAGATGCTAATGA+TGG - chr2.2:9234289-9234308 None:intergenic 30.0%
!! ATTCAATGAAGCTTTTTAGG+AGG + chr2.2:9235808-9235827 MS.gene043425:intron 30.0%
!! TCCTCATAAGAAGTGTTTAA+TGG - chr2.2:9236059-9236078 None:intergenic 30.0%
!!! CAATTTTATTGATTCCCCAA+AGG - chr2.2:9235414-9235433 None:intergenic 30.0%
!!! TTTTGGTGTCCCTATAAAAT+TGG + chr2.2:9235532-9235551 MS.gene043425:intron 30.0%
ACAAGTCATTGTAACATGTG+GGG + chr2.2:9233453-9233472 MS.gene043425:CDS 35.0%
ATATTCCGAACTGAGTTCTA+GGG + chr2.2:9234830-9234849 MS.gene043425:intron 35.0%
ATCACCTTTGGATCATAGTT+AGG - chr2.2:9232817-9232836 None:intergenic 35.0%
ATGGATCAAAATCAACAAGG+AGG + chr2.2:9232694-9232713 MS.gene043425:CDS 35.0%
ATGGCAAATCCTAAAGAACT+AGG - chr2.2:9233907-9233926 None:intergenic 35.0%
ATTACCTGATCAAGTAGATG+AGG + chr2.2:9233270-9233289 MS.gene043425:CDS 35.0%
ATTTCAAGATCCCAGAATTG+AGG - chr2.2:9235284-9235303 None:intergenic 35.0%
ATTTGAGCTAGTCAAGCTAA+AGG + chr2.2:9236368-9236387 MS.gene043425:CDS 35.0%
CCAAAAAACTTGAATAGTGG+GGG + chr2.2:9233642-9233661 MS.gene043425:intron 35.0%
CCAAATCGTAACAAAGATTG+CGG - chr2.2:9235194-9235213 None:intergenic 35.0%
CCAATCTCAAAGAAAACTCA+AGG - chr2.2:9232961-9232980 None:intergenic 35.0%
CTACAAGTCATTGTAACATG+TGG + chr2.2:9233451-9233470 MS.gene043425:CDS 35.0%
GAGAGTTGAGTTTAGAAAAG+AGG - chr2.2:9232789-9232808 None:intergenic 35.0%
GAGCCAAAAAACTTGAATAG+TGG + chr2.2:9233639-9233658 MS.gene043425:intron 35.0%
GCCAAAAAACTTGAATAGTG+GGG + chr2.2:9233641-9233660 MS.gene043425:intron 35.0%
GCCATTAAACACTTCTTATG+AGG + chr2.2:9236055-9236074 MS.gene043425:intron 35.0%
TATATTCCGAACTGAGTTCT+AGG + chr2.2:9234829-9234848 MS.gene043425:intron 35.0%
TCAAGGATTAGGTAAACTCT+TGG - chr2.2:9235345-9235364 None:intergenic 35.0%
TCACCTTTGGATCATAGTTA+GGG - chr2.2:9232816-9232835 None:intergenic 35.0%
TCATCTACTTGATCAGGTAA+TGG - chr2.2:9233271-9233290 None:intergenic 35.0%
TCTACTTTGGTTGAAGAAGA+AGG - chr2.2:9232724-9232743 None:intergenic 35.0%
TCTTCAAATTCTTCTTTGGC+TGG + chr2.2:9233541-9233560 MS.gene043425:CDS 35.0%
TGAAATTGGAAGTGAGAGTT+GGG + chr2.2:9235298-9235317 MS.gene043425:intron 35.0%
TGGCAAATCCTAAAGAACTA+GGG - chr2.2:9233906-9233925 None:intergenic 35.0%
TTCACTTAGATAGTAACGTG+AGG + chr2.2:9236087-9236106 MS.gene043425:intron 35.0%
TTGAAATTGGAAGTGAGAGT+TGG + chr2.2:9235297-9235316 MS.gene043425:intron 35.0%
TTTGAGCTAGTCAAGCTAAA+GGG + chr2.2:9236369-9236388 MS.gene043425:CDS 35.0%
! CGATTTGGAGAAACCAAAAT+AGG + chr2.2:9235206-9235225 MS.gene043425:CDS 35.0%
! TGTTACAATGACTTGTAGAG+TGG - chr2.2:9233449-9233468 None:intergenic 35.0%
! TTTCAATCTCTCGCCTATTT+TGG - chr2.2:9235222-9235241 None:intergenic 35.0%
!! AGATTGAAAGCATTTGTGGA+AGG + chr2.2:9235233-9235252 MS.gene043425:CDS 35.0%
!! ATCCAGAGCACCAATTTTAT+AGG - chr2.2:9235545-9235564 None:intergenic 35.0%
!! CAATTCTGGGATCTTGAAAT+TGG + chr2.2:9235284-9235303 MS.gene043425:CDS 35.0%
!! TCCAGAGCACCAATTTTATA+GGG - chr2.2:9235544-9235563 None:intergenic 35.0%
!!! CCTTGAGTTTTCTTTGAGAT+TGG + chr2.2:9232958-9232977 MS.gene043425:intron 35.0%
AAATGCTAACATGCGCTCTT+AGG + chr2.2:9233747-9233766 MS.gene043425:intron 40.0%
AACATCAACCGTACAATCAG+AGG - chr2.2:9234401-9234420 None:intergenic 40.0%
AATAAGAGATCACGTGATGG+TGG + chr2.2:9233364-9233383 MS.gene043425:CDS 40.0%
AATGCTAACATGCGCTCTTA+GGG + chr2.2:9233748-9233767 MS.gene043425:intron 40.0%
AGCTAAGCTAGACATGTTGA+AGG + chr2.2:9236341-9236360 MS.gene043425:CDS 40.0%
ATCCTAAGCTAAGCTTAGCT+CGG - chr2.2:9236148-9236167 None:intergenic 40.0%
ATCTTCCCTAGAACTCAGTT+CGG - chr2.2:9234838-9234857 None:intergenic 40.0%
ATGACACTTAGAAGGCAACA+CGG - chr2.2:9235873-9235892 None:intergenic 40.0%
ATTGCCTCATCTACTTGATC+AGG - chr2.2:9233277-9233296 None:intergenic 40.0%
CCGCAATCTTTGTTACGATT+TGG + chr2.2:9235191-9235210 MS.gene043425:CDS 40.0%
GCTAAGCTAGACATGTTGAA+GGG + chr2.2:9236342-9236361 MS.gene043425:CDS 40.0%
GCTTAGCTTAGGATGTTGAA+TGG + chr2.2:9236154-9236173 MS.gene043425:intron 40.0%
GGAAACACAACCTCAATTCT+GGG + chr2.2:9235271-9235290 MS.gene043425:CDS 40.0%
GGAAATAAGAGATCACGTGA+TGG + chr2.2:9233361-9233380 MS.gene043425:CDS 40.0%
GTATGTTGCAAACCCTTTCA+TGG - chr2.2:9234159-9234178 None:intergenic 40.0%
GTGTTGTGTGTGTGTATTCA+TGG - chr2.2:9234984-9235003 None:intergenic 40.0%
TAGCTTAGGATGTTGAATGG+TGG + chr2.2:9236157-9236176 MS.gene043425:intron 40.0%
TCTCCCTAACTATGATCCAA+AGG + chr2.2:9232810-9232829 MS.gene043425:CDS 40.0%
TGGAAACACAACCTCAATTC+TGG + chr2.2:9235270-9235289 MS.gene043425:CDS 40.0%
TTGAGCTAGTCAAGCTAAAG+GGG + chr2.2:9236370-9236389 MS.gene043425:CDS 40.0%
TTGTGTTTCCACATTGCTCA+CGG - chr2.2:9235261-9235280 None:intergenic 40.0%
! ACATACTAACTTGTGCCCTA+TGG + chr2.2:9234444-9234463 MS.gene043425:intron 40.0%
! CATACTAACTTGTGCCCTAT+GGG + chr2.2:9234445-9234464 MS.gene043425:intron 40.0%
! TAGCAAGACCCTAGTTCTTT+AGG + chr2.2:9233895-9233914 MS.gene043425:intron 40.0%
! TCCCTATAAAATTGGTGCTC+TGG + chr2.2:9235540-9235559 MS.gene043425:intron 40.0%
! TTGAATGGTGGAAATGAGCT+TGG + chr2.2:9236169-9236188 MS.gene043425:intron 40.0%
!! CGAGAGATTGAAAGCATTTG+TGG + chr2.2:9235229-9235248 MS.gene043425:CDS 40.0%
!!! CCCCCACTATTCAAGTTTTT+TGG - chr2.2:9233645-9233664 None:intergenic 40.0%
AAGATGAGCTAGGTGAGTTG+TGG + chr2.2:9236235-9236254 MS.gene043425:intron 45.0%
AAGCTAGACATGTTGAAGGG+TGG + chr2.2:9236345-9236364 MS.gene043425:CDS 45.0%
ACGAAGGAGTTGCATAGAGA+AGG - chr2.2:9235971-9235990 None:intergenic 45.0%
ACTCCACATGTCATGTACGA+AGG - chr2.2:9235987-9236006 None:intergenic 45.0%
ACTCCTTCGTACATGACATG+TGG + chr2.2:9235981-9236000 MS.gene043425:intron 45.0%
ACTGCATGAAGAAAACGTCG+AGG + chr2.2:9233513-9233532 MS.gene043425:CDS 45.0%
ATCAACCGTACAATCAGAGG+CGG - chr2.2:9234398-9234417 None:intergenic 45.0%
CTAGCTCATCTTGACCATTC+AGG - chr2.2:9236227-9236246 None:intergenic 45.0%
CTGAATGGTCAAGATGAGCT+AGG + chr2.2:9236225-9236244 MS.gene043425:intron 45.0%
CTGCATTGTGGAACTGAGTT+AGG + chr2.2:9236196-9236215 MS.gene043425:intron 45.0%
GGTCTAATTGTACGCAGTAG+CGG + chr2.2:9233617-9233636 MS.gene043425:intron 45.0%
TAGATGTCCAAGCCCATTAG+TGG + chr2.2:9235934-9235953 MS.gene043425:intron 45.0%
TCGTACATGACATGTGGAGT+TGG + chr2.2:9235987-9236006 MS.gene043425:intron 45.0%
TCTTCATACCGTACATGCTC+AGG + chr2.2:9233573-9233592 MS.gene043425:CDS 45.0%
TGAGCTAGGCATGACTTGAA+TGG + chr2.2:9236261-9236280 MS.gene043425:intron 45.0%
TGAGCTAGTCAAGCTAAAGG+GGG + chr2.2:9236371-9236390 MS.gene043425:CDS 45.0%
TGAGTTAGGCATGACCTGAA+TGG + chr2.2:9236210-9236229 MS.gene043425:intron 45.0%
TGTTGCAAACCCTTTCATGG+AGG - chr2.2:9234156-9234175 None:intergenic 45.0%
TTGAATGGTCATGCTGAGCT+AGG + chr2.2:9236276-9236295 MS.gene043425:intron 45.0%
! GAAGCTCTAGTTGACCTCAA+TGG + chr2.2:9236117-9236136 MS.gene043425:intron 45.0%
!! TATTTAAATAAATACATATT+TGG - chr2.2:9234955-9234974 None:intergenic 5.0%
!!! TATTTAATATTTTCAAAAAA+TGG - chr2.2:9233926-9233945 None:intergenic 5.0%
AACTTGTGCCCTATGGGCTT+AGG + chr2.2:9234451-9234470 MS.gene043425:intron 50.0%
ACTTGTGCCCTAAGCCCATA+GGG - chr2.2:9234462-9234481 None:intergenic 50.0%
ACTTGTGCCCTATGGGCTTA+GGG + chr2.2:9234452-9234471 MS.gene043425:intron 50.0%
AGCCACACACAAGGTTGTGT+GGG - chr2.2:9234375-9234394 None:intergenic 50.0%
AGCCGAGCTAAGCTTAGCTT+AGG + chr2.2:9236143-9236162 MS.gene043425:intron 50.0%
AGCTGAGCTAGGTATGCTGA+AGG + chr2.2:9236314-9236333 MS.gene043425:CDS 50.0%
GAGCAGCCAACTCCACTAAT+GGG - chr2.2:9235949-9235968 None:intergenic 50.0%
GCTGAGCTAGGCATGTTGAA+GGG + chr2.2:9236288-9236307 MS.gene043425:intron 50.0%
GCTGAGCTAGGTATGCTGAA+GGG + chr2.2:9236315-9236334 MS.gene043425:CDS 50.0%
GTAGCAGGCAACCGTATTGA+AGG - chr2.2:9234104-9234123 None:intergenic 50.0%
TCCAAGCCCATTAGTGGAGT+TGG + chr2.2:9235940-9235959 MS.gene043425:intron 50.0%
TGCTGAGCTAGGCATGTTGA+AGG + chr2.2:9236287-9236306 MS.gene043425:intron 50.0%
! AGCCATGAGCATTGTCGAGA+GGG - chr2.2:9235574-9235593 None:intergenic 50.0%
! TAGCCATGAGCATTGTCGAG+AGG - chr2.2:9235575-9235594 None:intergenic 50.0%
! TTAGCTCGGCTTGACCATTG+AGG - chr2.2:9236134-9236153 None:intergenic 50.0%
AAGGGGGAGTTCGACCTGAA+CGG + chr2.2:9236387-9236406 MS.gene043425:CDS 55.0%
AGCCCACACAACCTTGTGTG+TGG + chr2.2:9234370-9234389 MS.gene043425:intron 55.0%
CACTTGTGCCCTAAGCCCAT+AGG - chr2.2:9234463-9234482 None:intergenic 55.0%
GAGCCACACACAAGGTTGTG+TGG - chr2.2:9234376-9234395 None:intergenic 55.0%
GAGCTAGGCATGTTGAAGGG+TGG + chr2.2:9236291-9236310 MS.gene043425:intron 55.0%
GAGCTAGGTATGCTGAAGGG+TGG + chr2.2:9236318-9236337 MS.gene043425:CDS 55.0%
GAGCTTGGCATGCTGCATTG+TGG + chr2.2:9236184-9236203 MS.gene043425:intron 55.0%
GGAAGGATCCGTGAGCAATG+TGG + chr2.2:9235250-9235269 MS.gene043425:CDS 55.0%
GGAGCAGCCAACTCCACTAA+TGG - chr2.2:9235950-9235969 None:intergenic 55.0%
GTGAGTTGTGGAGCTGAGCT+AGG + chr2.2:9236247-9236266 MS.gene043425:intron 55.0%
TCGACCTGAACGGTGAAGCT+GGG + chr2.2:9236397-9236416 MS.gene043425:CDS 55.0%
TGGCTCCGCCTCTGATTGTA+CGG + chr2.2:9234390-9234409 MS.gene043425:intron 55.0%
TTCGACCTGAACGGTGAAGC+TGG + chr2.2:9236396-9236415 MS.gene043425:CDS 55.0%
TTGAAGGGTGGAGCTGAGCT+AGG + chr2.2:9236303-9236322 MS.gene043425:CDS 55.0%
! ATGAGCATTGTCGAGAGGGG+CGG - chr2.2:9235570-9235589 None:intergenic 55.0%
! GCCATGAGCATTGTCGAGAG+GGG - chr2.2:9235573-9235592 None:intergenic 55.0%
!! GCCAACTCCACTAATGGGCT+TGG - chr2.2:9235944-9235963 None:intergenic 55.0%
ACGGTGAAGCTGGGAGAGCA+TGG + chr2.2:9236406-9236425 MS.gene043425:CDS 60.0%
CTCTCCCAGCTTCACCGTTC+AGG - chr2.2:9236404-9236423 None:intergenic 60.0%
GCCCCTCTCGACAATGCTCA+TGG + chr2.2:9235569-9235588 MS.gene043425:intron 60.0%
GGTGGCTGCTCGAGTTCTTC+TGG + chr2.2:9233382-9233401 MS.gene043425:CDS 60.0%
CAGAGGCGGAGCCACACACA+AGG - chr2.2:9234384-9234403 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 9232694 9236431 9232694 ID=MS.gene043425
chr2.2 mRNA 9232694 9236431 9232694 ID=MS.gene043425.t1;Parent=MS.gene043425
chr2.2 exon 9232694 9232831 9232694 ID=MS.gene043425.t1.exon1;Parent=MS.gene043425.t1
chr2.2 CDS 9232694 9232831 9232694 ID=cds.MS.gene043425.t1;Parent=MS.gene043425.t1
chr2.2 exon 9233256 9233594 9233256 ID=MS.gene043425.t1.exon2;Parent=MS.gene043425.t1
chr2.2 CDS 9233256 9233594 9233256 ID=cds.MS.gene043425.t1;Parent=MS.gene043425.t1
chr2.2 exon 9235188 9235308 9235188 ID=MS.gene043425.t1.exon3;Parent=MS.gene043425.t1
chr2.2 CDS 9235188 9235308 9235188 ID=cds.MS.gene043425.t1;Parent=MS.gene043425.t1
chr2.2 exon 9236298 9236431 9236298 ID=MS.gene043425.t1.exon4;Parent=MS.gene043425.t1
chr2.2 CDS 9236298 9236431 9236298 ID=cds.MS.gene043425.t1;Parent=MS.gene043425.t1
Gene Sequence

>MS.gene043425

ATGGATCAAAATCAACAAGGAGGTCAACCTTCTTCTTCAACCAAAGTAGAAAGAAAGATCGTTGAGAAAAATAGAAGAAACCAAATGAAGATCCTCTTTTCTAAACTCAACTCTCTTCTCCCTAACTATGATCCAAAGGTGATTTTTTTATTTTATTTTATAAGCTAAGGGTGATCTGTTTAATTTCCATCACATTCATATGTATATTCATGTGAGGAATAAATAGACATTGAGTTTAATTTCCTGTAAAAAAAAAAAAATCGACCTTGAGTTTTCTTTGAGATTGGTAACTATAATATTATGTCACACATTGAGATCTAATCACAATTGTCACATATGTTTAACGGTTTCCAAATAAAAGGTGTTTACAAAACTCTTTTAGAGTGTTGTTGATGTAAAACCATTTTACACTTATTAGAGTGTACTATACCACAAGTAGAGTACCTATATATTCATATAGGTTCACCTCTTCTTTTAATTAATTAACAACTAATTAACACTATAAGCTCTTCTTGGATTGAATAACACATATTAATTTGGTGTTATTTTTCATTTAATGCAGGAATTAGCGTTACCATTACCTGATCAAGTAGATGAGGCAATAAACTACATAAAGAGCCTAGAGACAAATATAATGTTTGCAAAAGAGAAGAAAGAAAGTTTAATGGGAAATAAGAGATCACGTGATGGTGGCTGCTCGAGTTCTTCTGGAGCAATAAAGTTGCCAAAAATAGAGATTCATGAAATGGGAACCACTCTACAAGTCATTGTAACATGTGGGGTTGATGACCATTTTATTTTCTGTGAAGTTATGAGAATACTGCATGAAGAAAACGTCGAGGTTATCTCTTCAAATTCTTCTTTGGCTGGTGATTCACTTCTTCATACCGTACATGCTCAGGTATATATATACATACACACATGGTCTAATTGTACGCAGTAGCGGAGCCAAAAAACTTGAATAGTGGGGGCATCATATATAATTTGTAGCATTAAATATATATTACATTTCATTTTTTAAATCAGAAATACATATCACTATTGTTCTTTGGGAAATGCTAACATGCGCTCTTAGGGCACTTGATAAAAAATTTAAAGTAGAAATTTTATTTTTGAATTTGTTTATTCAACACATTGAAACATCAAAAAGTAACTTTATCTACATGAAAATTATCAATTGTTTCTATTTTAGGGCACTTGTTAGCAAGACCCTAGTTCTTTAGGATTTGCCATTTTTTGAAAATATTAAATAACGTTCTCATTTTCAATCTCAATAATAATACAAAAAAAAACCCTCTCTATATAAGTAACTAGATTTATAATTAATTGCACTTTTAATAACACAAATTTGTTAATTGCCTGATTTTTTCCTCCTAAAATTTATTGCAATAACAAATAATTGCAGTTCCTTCAATACGGTTGCCTGCTACCGTATAAAATTTATTGCAATAACAAATAACCTCCATGAAAGGGTTTGCAACATACATAAAATTCTTAAGCTCTTGCAATGTCTTCAACACATGCACCATCATATATGAGATTAAATCGTTTCATGCAGAGAAATAATACTCATTTGTTATGTCGATGATAAGCCATCATTAGCATCTATCATCAATTTTTATTAGATTAAAACTATCAATAATATAAAACAATTTTGAAAATTTAGTGTAGGCATGAGCCCACACAACCTTGTGTGTGGCTCCGCCTCTGATTGTACGGTTGATGTTACACTGCAAACCTTTAGAAAAACACATACTAACTTGTGCCCTATGGGCTTAGGGCACAAGTGAAGAAGCTTAAGATGTAATTAAAAAAAAAAAAACTTTTATATTGAAAAGATAACTTTTAAGATATTGAATGCATTCTTGTCAAAAAAAAAAAAAAAGGATATTGAATGCATAATTTTCAAGAAAAAACTTCTGCATTTTATTTCTTAACTTATGTCTAAGGACACAAGTTAGTTTTTCCCATTTTTTATGACATGTCATTTTTATTAATGATGTGAAATTAAATTTAATAGTGTGACAATGATGAATTAGACCATAAACCATGATCTAACATTTTTTTCTTTCTTTTAGTTTCAATGATTAAATGTAAACATATTCTTCTAATTCACATATGTGTGTTATAATAAATATATTCCGAACTGAGTTCTAGGGAAGATAAAAAATCCAAAAACTTAAAAAATGGAAATAAGAAATAGATAAACTAAAATCATGTTTCAGTTAAAAATAACTAAAAATGCAATTTTTTTAAAACCAAATATGTATTTATTTAAATATTAAAACCATGAATACACACACACAACACACACACACACACACACACACACACACACACACACACACACACACACACACATATATATATATATATATATATATATATTCTTTAGTCTTAACAATATTTTGTGAAGGAATCAATTATCTCTTCAACATTGAGAAACTCAATTATGAATGCTTTGTTTTCTGATTTGATTATTATTTTAATGTAGATTCCGCAATCTTTGTTACGATTTGGAGAAACCAAAATAGGCGAGAGATTGAAAGCATTTGTGGAAGGATCCGTGAGCAATGTGGAAACACAACCTCAATTCTGGGATCTTGAAATTGGAAGTGAGAGTTGGGAGCTTCTACATTCTGTAGTAACCAAGAGTTTACCTAATCCTTGATGAAGAATAGATTAAAGTATCTGGATCGATTCTAATATTTTTGGTACCTTTGGGGAATCAATAAAATTGTATCTCTTTTAATTATTTCAATGTTTTTATTCTTGCATTTTTTGTTGCAAATGATTGATTCATAATCAAGGTTCTTTAAAAATATATTTTTAATAAAATTTTGGTGTCCCTATAAAATTGGTGCTCTGGATGACCGCCCCTCTCGACAATGCTCATGGCTAACCATGTATGTATAGCATATATGTAGTGCAACTTCCCCTACAAGAATATATTCAGAATTCTAACTAATTTTAAGTGTAGTATAGAAATCCGTCACAAAAAAAAAACTTTTGTAGTATTTAGAAATCTTTCAATGTAAGAATAAAATTGGATAGAAATCGTTTTTATGAATGTTAATGTTCACGTATGTAAACACAAGTTACACTATATGAGTTATTCAATGAAGCTTTTTAGGAGGAGCTTCAAAAATACTCTAAAAAGCTAGAATAAATGGATTCCGTGTTGCCTTCTAAGTGTCATCTTTTATAGTCATCACTTTATTGACCTAATGGAACTCTTTTTAGATGTCCAAGCCCATTAGTGGAGTTGGCTGCTCCTTCTCTATGCAACTCCTTCGTACATGACATGTGGAGTTGGAACACATGTAACTTACGTCATTTGTTCGTGCATGACGTGTGACATGCCATTAAACACTTCTTATGAGGACTACAACTTTCACTTAGATAGTAACGTGAGGAAGAGACGAAGCTCTAGTTGACCTCAATGGTCAAGCCGAGCTAAGCTTAGCTTAGGATGTTGAATGGTGGAAATGAGCTTGGCATGCTGCATTGTGGAACTGAGTTAGGCATGACCTGAATGGTCAAGATGAGCTAGGTGAGTTGTGGAGCTGAGCTAGGCATGACTTGAATGGTCATGCTGAGCTAGGCATGTTGAAGGGTGGAGCTGAGCTAGGTATGCTGAAGGGTGGAGCTAAGCTAGACATGTTGAAGGGTGGATTTGAGCTAGTCAAGCTAAAGGGGGAGTTCGACCTGAACGGTGAAGCTGGGAGAGCATGGTGA
Protein sequence

>MS.gene043425.t1

MDQNQQGGQPSSSTKVERKIVEKNRRNQMKILFSKLNSLLPNYDPKELALPLPDQVDEAINYIKSLETNIMFAKEKKESLMGNKRSRDGGCSSSSGAIKLPKIEIHEMGTTLQVIVTCGVDDHFIFCEVMRILHEENVEVISSNSSLAGDSLLHTVHAQIPQSLLRFGETKIGERLKAFVEGSVSNVETQPQFWDLEIGSMLKGGAELGMLKGGAKLDMLKGGFELVKLKGEFDLNGEAGRAW