AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043464

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043464.t1 MTR_2g090745 83.092 414 14 3 1 373 31 429 0.0 661
MS.gene043464.t1 MTR_4g059870 53.667 300 105 8 1 280 62 347 2.13e-97 298
MS.gene043464.t1 MTR_2g099990 54.417 283 92 7 1 278 70 320 3.92e-95 292
MS.gene043464.t1 MTR_2g099990 54.417 283 92 7 1 278 1 251 3.99e-95 290
MS.gene043464.t1 MTR_8g017210 89.928 139 14 0 1 139 86 224 4.14e-93 288
MS.gene043464.t1 MTR_1g112270 72.515 171 44 1 1 168 68 238 5.01e-90 281
MS.gene043464.t1 MTR_1g112270 72.515 171 44 1 1 168 79 249 8.02e-90 281
MS.gene043464.t1 MTR_1g094115 42.369 439 156 13 1 358 71 493 9.44e-90 280
MS.gene043464.t1 MTR_1g094115 41.379 435 158 12 5 358 58 476 2.05e-86 271
MS.gene043464.t1 MTR_1g016010 82.979 141 23 1 1 140 65 205 4.47e-85 265
MS.gene043464.t1 MTR_2g093960 75.796 157 31 3 1 151 56 211 1.82e-83 260
MS.gene043464.t1 MTR_2g017720 90.099 101 5 1 1 101 337 432 1.82e-58 196
MS.gene043464.t1 MTR_3g102000 78.788 99 14 2 4 98 36 131 2.46e-48 163
MS.gene043464.t1 MTR_1g093095 33.557 149 86 5 1 137 110 257 1.35e-16 79.7
MS.gene043464.t1 MTR_5g030560 93.548 31 2 0 1 31 30 60 3.32e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043464.t1 AT5G44160 51.691 414 121 13 1 357 22 413 2.14e-116 345
MS.gene043464.t1 AT5G44160 51.691 414 121 13 1 357 61 452 2.87e-116 346
MS.gene043464.t1 AT1G03840 48.276 435 140 11 1 357 63 490 8.23e-116 346
MS.gene043464.t1 AT1G03840 48.276 435 140 11 1 357 65 492 9.85e-116 346
MS.gene043464.t1 AT3G50700 51.307 306 124 6 1 290 58 354 1.11e-93 288
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 1 325 1.51e-92 284
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 1 325 1.51e-92 284
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 78 402 2.86e-92 286
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 78 402 2.86e-92 286
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 78 402 2.86e-92 286
MS.gene043464.t1 AT2G02080 47.416 329 126 7 1 286 78 402 2.86e-92 286
MS.gene043464.t1 AT5G66730 87.050 139 18 0 1 139 56 194 3.20e-92 285
MS.gene043464.t1 AT1G55110 44.504 373 150 9 1 356 87 419 6.50e-91 280
MS.gene043464.t1 AT1G55110 44.504 373 150 9 1 356 87 419 6.50e-91 280
MS.gene043464.t1 AT1G55110 44.504 373 150 9 1 356 87 419 6.50e-91 280
MS.gene043464.t1 AT4G02670 80.405 148 28 1 1 147 79 226 3.46e-90 277
MS.gene043464.t1 AT4G02670 80.405 148 28 1 1 147 77 224 3.55e-90 277
MS.gene043464.t1 AT3G45260 50.987 304 120 8 1 276 63 365 6.00e-90 278
MS.gene043464.t1 AT3G45260 50.987 304 120 8 1 276 63 365 6.00e-90 278
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 94 233 2.47e-88 276
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 94 233 2.47e-88 276
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 75 214 2.48e-88 275
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 89 228 3.22e-88 275
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 81 220 4.39e-88 275
MS.gene043464.t1 AT3G13810 87.857 140 16 1 1 139 95 234 5.62e-88 275
MS.gene043464.t1 AT2G02070 81.295 139 26 0 1 139 76 214 5.68e-87 275
MS.gene043464.t1 AT2G02070 81.295 139 26 0 1 139 76 214 5.68e-87 275
MS.gene043464.t1 AT1G14580 80.576 139 27 0 1 139 77 215 1.39e-86 270
MS.gene043464.t1 AT1G14580 80.576 139 27 0 1 139 77 215 1.39e-86 270
MS.gene043464.t1 AT1G14580 80.576 139 27 0 1 139 91 229 2.22e-86 270
MS.gene043464.t1 AT5G60470 54.464 224 85 3 1 222 59 267 2.18e-83 261
MS.gene043464.t1 AT5G60470 54.464 224 85 3 1 222 68 276 2.40e-83 261
MS.gene043464.t1 AT5G60470 54.464 224 85 3 1 222 64 272 3.30e-83 261
MS.gene043464.t1 AT1G68130 65.152 132 44 2 1 130 65 196 2.30e-58 195
MS.gene043464.t1 AT1G25250 63.910 133 45 2 1 130 34 166 2.07e-57 191
MS.gene043464.t1 AT1G25250 63.910 133 45 2 1 130 57 189 2.49e-57 191
MS.gene043464.t1 AT2G01940 62.687 134 48 2 1 132 68 201 5.88e-57 192
MS.gene043464.t1 AT2G01940 61.481 135 49 3 1 132 68 202 4.32e-53 182
MS.gene043464.t1 AT1G68130 64.545 110 37 2 23 130 1 110 3.21e-45 158
MS.gene043464.t1 AT2G01940 62.500 112 40 2 23 132 1 112 6.84e-44 155
MS.gene043464.t1 AT1G25250 63.063 111 38 2 23 130 1 111 7.30e-44 154
MS.gene043464.t1 AT1G34370 32.584 178 102 4 1 160 29 206 3.68e-20 90.1
MS.gene043464.t1 AT1G34370 32.584 178 102 4 1 160 239 416 4.11e-20 92.0
MS.gene043464.t1 AT1G34370 32.584 178 102 4 1 160 239 416 4.11e-20 92.0
MS.gene043464.t1 AT5G22890 35.000 140 77 4 1 126 212 351 1.51e-19 89.4
MS.gene043464.t1 AT1G13290 34.337 166 87 9 1 154 96 251 6.75e-18 83.6
MS.gene043464.t1 AT1G34790 36.842 133 74 5 6 129 145 276 1.15e-17 83.2
MS.gene043464.t1 AT3G20880 34.783 138 80 5 1 129 251 387 1.93e-17 83.6
MS.gene043464.t1 AT1G51220 33.333 138 82 5 1 129 173 309 5.36e-17 81.6
MS.gene043464.t1 AT1G08290 37.500 136 72 6 5 129 179 312 2.43e-16 79.7
MS.gene043464.t1 AT3G57670 34.058 138 81 5 1 129 212 348 2.54e-16 80.1

Find 71 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATAGTGGTAATGAAAAT+TGG 0.170942 2.2:+8700607 MS.gene043464:CDS
CAATCATCCTTCAAGGGCTT+TGG 0.260521 2.2:+8699334 MS.gene043464:CDS
AGGGCTTTGGGAGACTTAAC+TGG 0.275104 2.2:+8699347 MS.gene043464:CDS
CTCTTGACATCCATTATTAG+AGG 0.287901 2.2:-8700649 None:intergenic
AATAAATTCTCCTCTAATAA+TGG 0.294626 2.2:+8700639 MS.gene043464:CDS
CTAGATCAGGTTTATCCCTT+TGG 0.309891 2.2:+8700493 MS.gene043464:intron
AACATAAATAACTCATTATT+AGG 0.317228 2.2:+8700342 MS.gene043464:CDS
TAATAATAACATCGATAATA+AGG 0.320132 2.2:+8700701 MS.gene043464:CDS
CTGCATGCGACGTCGTTTCA+TGG 0.334137 2.2:-8701027 None:intergenic
TCGATGTTATTATTAACTAA+TGG 0.340481 2.2:-8700693 None:intergenic
AAAGAACAAGTAAAGAAATA+AGG 0.347748 2.2:+8699273 MS.gene043464:CDS
TGCAGAATGAAGAGAGCGTT+TGG 0.365539 2.2:+8701045 MS.gene043464:CDS
TCAGCAGAGAGCTACATTTC+AGG 0.365625 2.2:+8700984 MS.gene043464:CDS
GTGTGTGAAATTTGTCTAAA+GGG 0.373283 2.2:+8699185 MS.gene043464:CDS
TCATTATTCCTTGGAAGCTT+AGG 0.378657 2.2:+8700870 MS.gene043464:CDS
GGTAATGAAAATTGGGTGTT+TGG 0.384447 2.2:+8700615 MS.gene043464:CDS
AATCATCCTTCAAGGGCTTT+GGG 0.387364 2.2:+8699335 MS.gene043464:CDS
GAAGAAACAGCTAGAATATA+CGG 0.388388 2.2:+8700312 MS.gene043464:CDS
GTACTTGCAACAAGTGATCT+TGG 0.393658 2.2:+8700960 MS.gene043464:CDS
TTTGATGATGAGATTGTGAT+TGG 0.412049 2.2:-8700776 None:intergenic
ATATAGTGGTAATGAAAATT+GGG 0.417202 2.2:+8700608 MS.gene043464:CDS
GCAGAATGAAGAGAGCGTTT+GGG 0.418512 2.2:+8701046 MS.gene043464:CDS
GACAAATTTCACACACGAAT+CGG 0.419935 2.2:-8699178 None:intergenic
CGTGTGTGAAATTTGTCTAA+AGG 0.423777 2.2:+8699184 MS.gene043464:CDS
TGCATGCGACGTCGTTTCAT+GGG 0.435103 2.2:-8701026 None:intergenic
CAGGGCATTCTGTGATGCCT+TGG 0.444003 2.2:+8700287 MS.gene043464:CDS
GTGTTCACAATCATCCTTCA+AGG 0.445387 2.2:+8699327 MS.gene043464:CDS
TTTATGCTGTTCAATCGGAT+TGG 0.446458 2.2:+8699435 MS.gene043464:CDS
TCAACTGAATCATTATTCCT+TGG 0.446740 2.2:+8700861 MS.gene043464:CDS
AAATAACTCATTATTAGGAA+TGG 0.455416 2.2:+8700347 MS.gene043464:CDS
AATAACTCATTATTAGGAAT+GGG 0.461219 2.2:+8700348 MS.gene043464:CDS
TTGCATGAGATTCATCAACT+TGG 0.465356 2.2:+8700561 MS.gene043464:CDS
AAGTCTCCCAAAGCCCTTGA+AGG 0.467713 2.2:-8699341 None:intergenic
GACGTGGACATAACCTTCCT+TGG 0.468137 2.2:+8699240 MS.gene043464:CDS
TTGTGAACACATGTCTTCTC+TGG 0.471074 2.2:-8699314 None:intergenic
GTTCTTTGTTTCAGCTTCCA+AGG 0.486730 2.2:-8699257 None:intergenic
AGAGCGTTTGGGGACAAACT+AGG 0.488786 2.2:+8701057 MS.gene043464:CDS
TACTTGCAACAAGTGATCTT+GGG 0.493836 2.2:+8700961 MS.gene043464:CDS
AATAGTCCAAGTCAAGATCA+TGG 0.501314 2.2:+8700903 MS.gene043464:CDS
GGAAGCACGGTGAGAAGAAG+TGG 0.504824 2.2:+8699390 MS.gene043464:CDS
TCTAGCTGTTTCTTCTGCCA+AGG 0.509785 2.2:-8700304 None:intergenic
GAGCGTTTGGGGACAAACTA+GGG 0.510953 2.2:+8701058 MS.gene043464:CDS
TGCATGAGATTCATCAACTT+GGG 0.511043 2.2:+8700562 MS.gene043464:CDS
TTTGTTTCAGCTTCCAAGGA+AGG 0.514000 2.2:-8699253 None:intergenic
TTATTCCCATGATCTTGACT+TGG 0.518278 2.2:-8700909 None:intergenic
GGGATAGCTTTGTCACTCAC+AGG 0.519723 2.2:+8700268 MS.gene043464:CDS
AGCACAGGAGCAATATATAG+TGG 0.537235 2.2:+8700594 MS.gene043464:CDS
GATTGATGAACTGTGGCAAA+TGG 0.542982 2.2:-8701106 None:intergenic
ATCTCAAACCTAAGCTTCCA+AGG 0.557018 2.2:-8700878 None:intergenic
AACATCTCATGACACAATGT+TGG 0.561312 2.2:+8700524 MS.gene043464:CDS
GCATGCGACGTCGTTTCATG+GGG 0.563017 2.2:-8701025 None:intergenic
TGTTGCTGACATGTTTGCTG+AGG 0.569109 2.2:-8700802 None:intergenic
ATAGTCCAAGTCAAGATCAT+GGG 0.586895 2.2:+8700904 MS.gene043464:CDS
TGCCACAGTTCATCAATCAA+CGG 0.590617 2.2:+8701111 MS.gene043464:CDS
TGTTCACAATCATCCTTCAA+GGG 0.591498 2.2:+8699328 MS.gene043464:CDS
GGGCTTTGGGAGACTTAACT+GGG 0.593735 2.2:+8699348 MS.gene043464:CDS
CTTGGGTCTACTACAAGCAC+AGG 0.600800 2.2:+8700579 MS.gene043464:CDS
CAGAATGAAGAGAGCGTTTG+GGG 0.617026 2.2:+8701047 MS.gene043464:CDS
GAGATGTTTGAGACATCCAA+AGG 0.619714 2.2:-8700509 None:intergenic
ACAGTTCATCAATCAACGGA+TGG 0.619902 2.2:+8701115 MS.gene043464:CDS
AGATGTTTGAGACATCCAAA+GGG 0.623148 2.2:-8700508 None:intergenic
ATGCGACGTCGTTTCATGGG+GGG 0.625847 2.2:-8701023 None:intergenic
GGAATGGGAAGTAACAACAT+TGG 0.637989 2.2:+8700363 MS.gene043464:CDS
CATGCGACGTCGTTTCATGG+GGG 0.652534 2.2:-8701024 None:intergenic
TTGGTGTTGTGAGCTATACA+AGG 0.662064 2.2:-8700757 None:intergenic
AAAGCTCACTCAAAGACTTG+TGG 0.665203 2.2:+8699458 MS.gene043464:CDS
TGTTGTGAGCTATACAAGGA+AGG 0.666542 2.2:-8700753 None:intergenic
GGATAGCTTTGTCACTCACA+GGG 0.679845 2.2:+8700269 MS.gene043464:CDS
AGAGAATACAAATGTGACTG+TGG 0.689229 2.2:+8699485 MS.gene043464:CDS
AATCTTCAACTTCATCGACG+TGG 0.715188 2.2:+8699224 MS.gene043464:CDS
ATCCGTTGATTGATGAACTG+TGG 0.722662 2.2:-8701113 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAACAATAAATAAAAAA+TGG - chr2.2:8700217-8700236 None:intergenic 10.0%
!! ATCAACAATAAATAAAAAAT+GGG - chr2.2:8700216-8700235 None:intergenic 10.0%
!! TTATATGGAATAAAATAAAA+GGG + chr2.2:8699806-8699825 MS.gene043464:intron 10.0%
!! AAAAAGGTAATAAACTTAAA+AGG - chr2.2:8699634-8699653 None:intergenic 15.0%
!! AAAATTAGTATAACATAACT+AGG - chr2.2:8700181-8700200 None:intergenic 15.0%
!! AACATAAATAACTCATTATT+AGG + chr2.2:8700342-8700361 MS.gene043464:CDS 15.0%
!! CTTATATGGAATAAAATAAA+AGG + chr2.2:8699805-8699824 MS.gene043464:intron 15.0%
!! GATTACTTATCAATTTAATT+TGG + chr2.2:8700233-8700252 MS.gene043464:intron 15.0%
!! TAATAATAACATCGATAATA+AGG + chr2.2:8700701-8700720 MS.gene043464:CDS 15.0%
!!! TGTTTATAATTTAGAATAGA+GGG + chr2.2:8700132-8700151 MS.gene043464:intron 15.0%
!!! TTGTTTATAATTTAGAATAG+AGG + chr2.2:8700131-8700150 MS.gene043464:intron 15.0%
!! AAAAATGAGTTCCTATATTT+GGG - chr2.2:8700419-8700438 None:intergenic 20.0%
!! AAAGAACAAGTAAAGAAATA+AGG + chr2.2:8699273-8699292 MS.gene043464:CDS 20.0%
!! AAATAACTCATTATTAGGAA+TGG + chr2.2:8700347-8700366 MS.gene043464:CDS 20.0%
!! AATAAATTCTCCTCTAATAA+TGG + chr2.2:8700639-8700658 MS.gene043464:CDS 20.0%
!! AATAACTCATTATTAGGAAT+GGG + chr2.2:8700348-8700367 MS.gene043464:CDS 20.0%
!! AATTACTTTGACTAATTTGA+GGG + chr2.2:8699759-8699778 MS.gene043464:intron 20.0%
!! CAAAAATGTACATTTACTTA+TGG - chr2.2:8700114-8700133 None:intergenic 20.0%
!! TAATTACTTTGACTAATTTG+AGG + chr2.2:8699758-8699777 MS.gene043464:intron 20.0%
!! TCGATGTTATTATTAACTAA+TGG - chr2.2:8700696-8700715 None:intergenic 20.0%
!!! AAAATTTTATGCTGTTCAAT+CGG + chr2.2:8699430-8699449 MS.gene043464:CDS 20.0%
!!! ATATAGTGGTAATGAAAATT+GGG + chr2.2:8700608-8700627 MS.gene043464:CDS 20.0%
!!! TATATAGTGGTAATGAAAAT+TGG + chr2.2:8700607-8700626 MS.gene043464:CDS 20.0%
! AAATAATGATCACAAAGGAA+AGG - chr2.2:8699732-8699751 None:intergenic 25.0%
! AACAGAAATAATGATCACAA+AGG - chr2.2:8699737-8699756 None:intergenic 25.0%
! ATAACATAACTAGGTTGTAA+GGG - chr2.2:8700172-8700191 None:intergenic 25.0%
! GAAAAATGAGTTCCTATATT+TGG - chr2.2:8700420-8700439 None:intergenic 25.0%
! GTTATTAGCTACTAATAGTT+TGG + chr2.2:8699676-8699695 MS.gene043464:intron 25.0%
! TATAACATAACTAGGTTGTA+AGG - chr2.2:8700173-8700192 None:intergenic 25.0%
!! AATTCTTTTGTCCCAAATAT+AGG + chr2.2:8700405-8700424 MS.gene043464:intron 25.0%
!! ATCATGGGAATAAATTTTGT+GGG + chr2.2:8700919-8700938 MS.gene043464:CDS 25.0%
!! TGGAAAATAGAAGTCTTATA+TGG + chr2.2:8699791-8699810 MS.gene043464:intron 25.0%
!!! AGTTTTTCTCTCTAAAACAA+CGG + chr2.2:8699897-8699916 MS.gene043464:intron 25.0%
ACTAATCAAACATCTAGATC+AGG + chr2.2:8700480-8700499 MS.gene043464:intron 30.0%
CCAAGAGAATTTATCCAATT+AGG - chr2.2:8699976-8699995 None:intergenic 30.0%
CCTAATTGGATAAATTCTCT+TGG + chr2.2:8699973-8699992 MS.gene043464:intron 30.0%
GAAGAAACAGCTAGAATATA+CGG + chr2.2:8700312-8700331 MS.gene043464:CDS 30.0%
GTGTGTGAAATTTGTCTAAA+GGG + chr2.2:8699185-8699204 MS.gene043464:CDS 30.0%
TCAACTGAATCATTATTCCT+TGG + chr2.2:8700861-8700880 MS.gene043464:CDS 30.0%
! AATAAATTTTGTGGGGTGTA+TGG + chr2.2:8700927-8700946 MS.gene043464:CDS 30.0%
! GATCATGGGAATAAATTTTG+TGG + chr2.2:8700918-8700937 MS.gene043464:CDS 30.0%
! GCTACTAATAGTTTGGATTT+TGG + chr2.2:8699683-8699702 MS.gene043464:intron 30.0%
! TCATGGGAATAAATTTTGTG+GGG + chr2.2:8700920-8700939 MS.gene043464:CDS 30.0%
! TTGTCTAAAGGGTTTTCAAA+GGG + chr2.2:8699196-8699215 MS.gene043464:CDS 30.0%
! TTTGTCTAAAGGGTTTTCAA+AGG + chr2.2:8699195-8699214 MS.gene043464:CDS 30.0%
!! AATTTAATTTGGTGCAACAC+AGG + chr2.2:8700244-8700263 MS.gene043464:intron 30.0%
!! TTTGATGATGAGATTGTGAT+TGG - chr2.2:8700779-8700798 None:intergenic 30.0%
AAAGGGACTTAAAACCTCAA+CGG + chr2.2:8699823-8699842 MS.gene043464:intron 35.0%
AAAGTAGAGTGAAGCCTAAT+TGG + chr2.2:8699959-8699978 MS.gene043464:intron 35.0%
AACATCTCATGACACAATGT+TGG + chr2.2:8700524-8700543 MS.gene043464:CDS 35.0%
AATAGTCCAAGTCAAGATCA+TGG + chr2.2:8700903-8700922 MS.gene043464:CDS 35.0%
AGAGAATACAAATGTGACTG+TGG + chr2.2:8699485-8699504 MS.gene043464:CDS 35.0%
ATAGTCCAAGTCAAGATCAT+GGG + chr2.2:8700904-8700923 MS.gene043464:CDS 35.0%
CCGACGCTAAAATTTCAATA+GGG + chr2.2:8700036-8700055 MS.gene043464:intron 35.0%
CGACGCTAAAATTTCAATAG+GGG + chr2.2:8700037-8700056 MS.gene043464:intron 35.0%
CGTGTGTGAAATTTGTCTAA+AGG + chr2.2:8699184-8699203 MS.gene043464:CDS 35.0%
CTCTTGACATCCATTATTAG+AGG - chr2.2:8700652-8700671 None:intergenic 35.0%
GACAAATTTCACACACGAAT+CGG - chr2.2:8699181-8699200 None:intergenic 35.0%
GGTAATGAAAATTGGGTGTT+TGG + chr2.2:8700615-8700634 MS.gene043464:CDS 35.0%
TAATTTGAGGGTTGAGATTG+TGG + chr2.2:8699771-8699790 MS.gene043464:intron 35.0%
TACTTGCAACAAGTGATCTT+GGG + chr2.2:8700961-8700980 MS.gene043464:CDS 35.0%
TCATTATTCCTTGGAAGCTT+AGG + chr2.2:8700870-8700889 MS.gene043464:CDS 35.0%
TGCAAAAAGCTGCTCAAATT+GGG + chr2.2:8700832-8700851 MS.gene043464:CDS 35.0%
TGCATGAGATTCATCAACTT+GGG + chr2.2:8700562-8700581 MS.gene043464:CDS 35.0%
TGTTCACAATCATCCTTCAA+GGG + chr2.2:8699328-8699347 MS.gene043464:CDS 35.0%
TTATTCCCATGATCTTGACT+TGG - chr2.2:8700912-8700931 None:intergenic 35.0%
TTGCAAAAAGCTGCTCAAAT+TGG + chr2.2:8700831-8700850 MS.gene043464:CDS 35.0%
TTGCATGAGATTCATCAACT+TGG + chr2.2:8700561-8700580 MS.gene043464:CDS 35.0%
TTTATGCTGTTCAATCGGAT+TGG + chr2.2:8699435-8699454 MS.gene043464:CDS 35.0%
! AGATGTTTGAGACATCCAAA+GGG - chr2.2:8700511-8700530 None:intergenic 35.0%
!! ACTTGTCTAGTGCTCTAAAA+TGG + chr2.2:8699591-8699610 MS.gene043464:intron 35.0%
!! CCCTATTGAAATTTTAGCGT+CGG - chr2.2:8700039-8700058 None:intergenic 35.0%
!! TCTTGATTTGTGCATGAGAT+TGG - chr2.2:8700456-8700475 None:intergenic 35.0%
!!! GGATAAAGAAGCACTTTTGT+AGG + chr2.2:8699369-8699388 MS.gene043464:CDS 35.0%
AAAGCTCACTCAAAGACTTG+TGG + chr2.2:8699458-8699477 MS.gene043464:CDS 40.0%
AATCATCCTTCAAGGGCTTT+GGG + chr2.2:8699335-8699354 MS.gene043464:CDS 40.0%
AATCTTCAACTTCATCGACG+TGG + chr2.2:8699224-8699243 MS.gene043464:CDS 40.0%
ACAGTTCATCAATCAACGGA+TGG + chr2.2:8701115-8701134 MS.gene043464:CDS 40.0%
AGCACAGGAGCAATATATAG+TGG + chr2.2:8700594-8700613 MS.gene043464:CDS 40.0%
ATCTCAAACCTAAGCTTCCA+AGG - chr2.2:8700881-8700900 None:intergenic 40.0%
CGTTGAAGATAGCTGCAAAA+AGG - chr2.2:8699650-8699669 None:intergenic 40.0%
CTAGATCAGGTTTATCCCTT+TGG + chr2.2:8700493-8700512 MS.gene043464:intron 40.0%
GATTGATGAACTGTGGCAAA+TGG - chr2.2:8701109-8701128 None:intergenic 40.0%
GCCGACGCTAAAATTTCAAT+AGG + chr2.2:8700035-8700054 MS.gene043464:intron 40.0%
GGAATGGGAAGTAACAACAT+TGG + chr2.2:8700363-8700382 MS.gene043464:CDS 40.0%
GTACTTGCAACAAGTGATCT+TGG + chr2.2:8700960-8700979 MS.gene043464:CDS 40.0%
GTGTTCACAATCATCCTTCA+AGG + chr2.2:8699327-8699346 MS.gene043464:CDS 40.0%
TGCCACAGTTCATCAATCAA+CGG + chr2.2:8701111-8701130 MS.gene043464:CDS 40.0%
TGTTGTGAGCTATACAAGGA+AGG - chr2.2:8700756-8700775 None:intergenic 40.0%
TTGTGAACACATGTCTTCTC+TGG - chr2.2:8699317-8699336 None:intergenic 40.0%
TTTGTTTCAGCTTCCAAGGA+AGG - chr2.2:8699256-8699275 None:intergenic 40.0%
! AAGCACTTTTGTAGGAAGCA+CGG + chr2.2:8699377-8699396 MS.gene043464:CDS 40.0%
! ACTGTGGCACTATCTTTTCA+AGG + chr2.2:8699501-8699520 MS.gene043464:CDS 40.0%
! ATCCGTTGATTGATGAACTG+TGG - chr2.2:8701116-8701135 None:intergenic 40.0%
! GAGATGTTTGAGACATCCAA+AGG - chr2.2:8700512-8700531 None:intergenic 40.0%
! GGACAAACTAGGGATTTTCT+TGG + chr2.2:8701068-8701087 MS.gene043464:CDS 40.0%
! GTTCTTTGTTTCAGCTTCCA+AGG - chr2.2:8699260-8699279 None:intergenic 40.0%
!! ACTAGGGATTTTCTTGGTGT+TGG + chr2.2:8701074-8701093 MS.gene043464:CDS 40.0%
!! TAATTTGGTGCAACACAGGA+GGG + chr2.2:8700248-8700267 MS.gene043464:intron 40.0%
!! TTAATTTGGTGCAACACAGG+AGG + chr2.2:8700247-8700266 MS.gene043464:intron 40.0%
!! TTGGTGTTGTGAGCTATACA+AGG - chr2.2:8700760-8700779 None:intergenic 40.0%
CAATCATCCTTCAAGGGCTT+TGG + chr2.2:8699334-8699353 MS.gene043464:CDS 45.0%
TCAGCAGAGAGCTACATTTC+AGG + chr2.2:8700984-8701003 MS.gene043464:CDS 45.0%
TCGAGTACTATACTCCGTTG+AGG - chr2.2:8699840-8699859 None:intergenic 45.0%
! TGTTGCTGACATGTTTGCTG+AGG - chr2.2:8700805-8700824 None:intergenic 45.0%
!! CAGAATGAAGAGAGCGTTTG+GGG + chr2.2:8701047-8701066 MS.gene043464:CDS 45.0%
!! GCAGAATGAAGAGAGCGTTT+GGG + chr2.2:8701046-8701065 MS.gene043464:CDS 45.0%
!! GGATAGCTTTGTCACTCACA+GGG + chr2.2:8700269-8700288 MS.gene043464:CDS 45.0%
!! TCTAGCTGTTTCTTCTGCCA+AGG - chr2.2:8700307-8700326 None:intergenic 45.0%
!! TGCAGAATGAAGAGAGCGTT+TGG + chr2.2:8701045-8701064 MS.gene043464:CDS 45.0%
!!! AGGGATTTTCTTGGTGTTGG+TGG + chr2.2:8701077-8701096 MS.gene043464:CDS 45.0%
AAGTCTCCCAAAGCCCTTGA+AGG - chr2.2:8699344-8699363 None:intergenic 50.0%
CTTGGGTCTACTACAAGCAC+AGG + chr2.2:8700579-8700598 MS.gene043464:CDS 50.0%
GACGTGGACATAACCTTCCT+TGG + chr2.2:8699240-8699259 MS.gene043464:CDS 50.0%
GAGCGTTTGGGGACAAACTA+GGG + chr2.2:8701058-8701077 MS.gene043464:CDS 50.0%
TGCATGCGACGTCGTTTCAT+GGG - chr2.2:8701029-8701048 None:intergenic 50.0%
! AGAGCGTTTGGGGACAAACT+AGG + chr2.2:8701057-8701076 MS.gene043464:CDS 50.0%
!! AGGGCTTTGGGAGACTTAAC+TGG + chr2.2:8699347-8699366 MS.gene043464:CDS 50.0%
!! GGGATAGCTTTGTCACTCAC+AGG + chr2.2:8700268-8700287 MS.gene043464:CDS 50.0%
!! GGGCTTTGGGAGACTTAACT+GGG + chr2.2:8699348-8699367 MS.gene043464:CDS 50.0%
ATGCGACGTCGTTTCATGGG+GGG - chr2.2:8701026-8701045 None:intergenic 55.0%
CATGCGACGTCGTTTCATGG+GGG - chr2.2:8701027-8701046 None:intergenic 55.0%
CTGCATGCGACGTCGTTTCA+TGG - chr2.2:8701030-8701049 None:intergenic 55.0%
GCATGCGACGTCGTTTCATG+GGG - chr2.2:8701028-8701047 None:intergenic 55.0%
! CAGGGCATTCTGTGATGCCT+TGG + chr2.2:8700287-8700306 MS.gene043464:CDS 55.0%
! GGAAGCACGGTGAGAAGAAG+TGG + chr2.2:8699390-8699409 MS.gene043464:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 8699167 8701148 8699167 ID=MS.gene043464
chr2.2 mRNA 8699167 8701148 8699167 ID=MS.gene043464.t1;Parent=MS.gene043464
chr2.2 exon 8699167 8699522 8699167 ID=MS.gene043464.t1.exon1;Parent=MS.gene043464.t1
chr2.2 CDS 8699167 8699522 8699167 ID=cds.MS.gene043464.t1;Parent=MS.gene043464.t1
chr2.2 exon 8700266 8700384 8700266 ID=MS.gene043464.t1.exon2;Parent=MS.gene043464.t1
chr2.2 CDS 8700266 8700384 8700266 ID=cds.MS.gene043464.t1;Parent=MS.gene043464.t1
chr2.2 exon 8700502 8701148 8700502 ID=MS.gene043464.t1.exon3;Parent=MS.gene043464.t1
chr2.2 CDS 8700502 8701148 8700502 ID=cds.MS.gene043464.t1;Parent=MS.gene043464.t1
Gene Sequence

>MS.gene043464

ATGGCAACAAACCGATTCGTGTGTGAAATTTGTCTAAAGGGTTTTCAAAGGGATCAAAATCTTCAACTTCATCGACGTGGACATAACCTTCCTTGGAAGCTGAAACAAAGAACAAGTAAAGAAATAAGGAAGAGAGTGTATGTGTGTCCAGAGAAGACATGTGTTCACAATCATCCTTCAAGGGCTTTGGGAGACTTAACTGGGATAAAGAAGCACTTTTGTAGGAAGCACGGTGAGAAGAAGTGGAAGTGTGAGAAGTGCTCAAAATTTTATGCTGTTCAATCGGATTGGAAAGCTCACTCAAAGACTTGTGGTACTAGAGAATACAAATGTGACTGTGGCACTATCTTTTCAAGGTAATTAATTTCTCTTTTCTTAACTCTCAGATATGTCATATACTCAAAAAAATTATGTCAAAGTTGTAACTTGTCTAGTGCTCTAAAATGGATCATCATTGAAAGAATCCTTTTAAGTTTATTACCTTTTTGCAGCTATCTTCAACGACAAAAGTTATTAGCTACTAATAGTTTGGATTTTGGAATTATCTAATTATATTAATCTACCTTTCCTTTGTGATCATTATTTCTGTTTTAATTACTTTGACTAATTTGAGGGTTGAGATTGTGGAAAATAGAAGTCTTATATGGAATAAAATAAAAGGGACTTAAAACCTCAACGGAGTATAGTACTCGAAGTGATCGTTACATTTAGAGAGAAAAACAAATCTACTAGTTTTTCTCTCTAAAACAACGGTCTCTAGCTACTATTGATCACTTAAGATTAAAGAGTTGCAAAGTAGAGTGAAGCCTAATTGGATAAATTCTCTTGGATCATACTAATATTCAATAAGCAAATACTTTTCAATTGAGCCGACGCTAAAATTTCAATAGGGGTTTACACTTACTCTAATCTTAACTATAGACAAAAATACACATTTATTATGTCCATAAGTAAATGTACATTTTTGTTTATAATTTAGAATAGAGGGAATACAAGCTATGACCCTTACAACCTAGTTATGTTATACTAATTTTCTTACATTAATTCCCATTTTTTATTTATTGTTGATTACTTATCAATTTAATTTGGTGCAACACAGGAGGGATAGCTTTGTCACTCACAGGGCATTCTGTGATGCCTTGGCAGAAGAAACAGCTAGAATATACGGTTCAACAAACATAAATAACTCATTATTAGGAATGGGAAGTAACAACATTGGTAATTACAACATCATGCAAAATTCTTTTGTCCCAAATATAGGAACTCATTTTTCTTCAATTTTCAAACCAATCTCATGCACAAATCAAGAAACAACTAATCAAACATCTAGATCAGGTTTATCCCTTTGGATGTCTCAAACATCTCATGACACAATGTTGGCTAATAATAGCAATTTGCATGAGATTCATCAACTTGGGTCTACTACAAGCACAGGAGCAATATATAGTGGTAATGAAAATTGGGTGTTTGGAAATAAATTCTCCTCTAATAATGGATGTCAAGAGTTAACTAGTTCTGTTTCACTTCCATTAGTTAATAATAACATCGATAATAAGGATAGTACTACAAACAATGTTGTTAGTGTTCCTTCCTTGTATAGCTCACAACACCAATCACAATCTCATCATCAAACAACCTCAGCAAACATGTCAGCAACAGCTTTGTTGCAAAAAGCTGCTCAAATTGGGACAACTTCAACTGAATCATTATTCCTTGGAAGCTTAGGTTTGAGATGCAATAGTCCAAGTCAAGATCATGGGAATAAATTTTGTGGGGTGTATGGTTCAAGTTCAGTACTTGCAACAAGTGATCTTGGGTCAGCAGAGAGCTACATTTCAGGTGAATTGTCACAAATGCCCCCCATGAAACGACGTCGCATGCAGAATGAAGAGAGCGTTTGGGGACAAACTAGGGATTTTCTTGGTGTTGGTGGTGCTAACCATTTGCCACAGTTCATCAATCAACGGATGGATTTGATTTGA
Protein sequence

>MS.gene043464.t1

MATNRFVCEICLKGFQRDQNLQLHRRGHNLPWKLKQRTSKEIRKRVYVCPEKTCVHNHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHSKTCGTREYKCDCGTIFSRRDSFVTHRAFCDALAEETARIYGSTNINNSLLGMGSNNIGLSLWMSQTSHDTMLANNSNLHEIHQLGSTTSTGAIYSGNENWVFGNKFSSNNGCQELTSSVSLPLVNNNIDNKDSTTNNVVSVPSLYSSQHQSQSHHQTTSANMSATALLQKAAQIGTTSTESLFLGSLGLRCNSPSQDHGNKFCGVYGSSSVLATSDLGSAESYISGELSQMPPMKRRRMQNEESVWGQTRDFLGVGGANHLPQFINQRMDLI