AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043709


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043709.t1 MTR_7g050560 82.840 169 28 1 1 168 1 169 4.92e-98 286
MS.gene043709.t1 MTR_7g050720 65.000 180 45 1 5 166 2 181 4.65e-78 235
MS.gene043709.t1 MTR_7g050710 62.842 183 52 2 1 168 1 182 3.06e-74 225
MS.gene043709.t1 MTR_7g050590 69.032 155 32 2 28 166 1 155 1.97e-72 220
MS.gene043709.t1 MTR_3g098790 61.878 181 47 3 1 166 1 174 3.04e-72 221
MS.gene043709.t1 MTR_7g050580 65.644 163 34 3 1 148 1 156 2.49e-66 205
MS.gene043709.t1 MTR_7g050640 64.602 113 40 0 1 113 1 113 6.51e-47 149
MS.gene043709.t1 MTR_3g061330 53.153 111 49 2 1 111 1 108 6.23e-35 122
MS.gene043709.t1 MTR_8g103940 51.485 101 49 0 11 111 37 137 5.44e-34 125
MS.gene043709.t1 MTR_3g061290 53.922 102 45 1 10 111 9 108 8.33e-32 113
MS.gene043709.t1 MTR_3g061310 50.000 106 51 1 6 111 5 108 1.04e-30 112
MS.gene043709.t1 MTR_3g061370 53.465 101 45 1 11 111 7 105 2.32e-30 112
MS.gene043709.t1 MTR_3g061340 53.398 103 45 2 10 111 9 109 4.37e-30 110
MS.gene043709.t1 MTR_3g061320 49.541 109 53 1 3 111 2 108 4.59e-30 110
MS.gene043709.t1 MTR_1g035460 55.814 86 38 0 28 113 1 86 1.43e-29 112
MS.gene043709.t1 MTR_3g061350 48.649 111 54 2 1 111 1 108 2.02e-28 107
MS.gene043709.t1 MTR_5g044670 43.434 99 56 0 13 111 16 114 1.71e-27 105
MS.gene043709.t1 MTR_1g108780 55.422 83 37 0 29 111 13 95 2.27e-27 106
MS.gene043709.t1 MTR_3g061220 46.226 106 42 2 6 111 5 95 2.02e-26 97.1
MS.gene043709.t1 MTR_6g043890 43.636 110 57 2 11 117 13 120 1.43e-24 97.8
MS.gene043709.t1 MTR_3g005480 34.400 125 74 2 13 137 21 137 2.33e-24 95.9
MS.gene043709.t1 MTR_4g119990 43.810 105 58 1 11 114 13 117 1.09e-22 93.2
MS.gene043709.t1 MTR_3g005490 33.929 112 71 1 13 124 21 129 6.07e-22 89.4
MS.gene043709.t1 MTR_1g034240 40.404 99 59 0 13 111 7 105 7.62e-22 91.3
MS.gene043709.t1 MTR_4g068320 31.646 158 104 2 13 166 6 163 1.25e-21 90.5
MS.gene043709.t1 MTR_5g070440 36.275 102 65 0 13 114 21 122 1.59e-21 88.6
MS.gene043709.t1 MTR_1g035640 47.475 99 46 2 13 111 9 101 1.82e-21 90.5
MS.gene043709.t1 MTR_1g050845 37.589 141 79 3 10 143 6 144 7.85e-21 89.0
MS.gene043709.t1 MTR_3g005460 34.821 112 70 1 13 124 25 133 2.31e-20 87.4
MS.gene043709.t1 MTR_3g005460 35.652 115 70 2 11 124 260 371 6.29e-19 83.2
MS.gene043709.t1 MTR_3g005495 39.216 102 62 0 13 114 243 344 7.01e-20 85.9
MS.gene043709.t1 MTR_3g005495 35.417 96 59 1 29 124 29 121 2.12e-18 81.6
MS.gene043709.t1 MTR_1g035620 41.176 102 58 1 10 111 6 105 7.86e-20 85.5
MS.gene043709.t1 MTR_1g035650 39.216 102 60 1 10 111 6 105 9.11e-20 82.4
MS.gene043709.t1 MTR_1g035480 35.714 140 79 3 9 148 13 141 1.43e-19 81.3
MS.gene043709.t1 MTR_1g035570 44.444 90 48 1 22 111 14 101 2.63e-19 84.0
MS.gene043709.t1 MTR_1g035440 39.815 108 58 2 11 114 13 117 3.58e-19 81.6
MS.gene043709.t1 MTR_3g005420 35.714 112 69 1 13 124 289 397 4.58e-18 80.9
MS.gene043709.t1 MTR_3g005420 28.788 132 84 3 13 135 40 170 1.75e-14 70.5
MS.gene043709.t1 MTR_1g033740 31.677 161 96 4 9 167 18 166 2.20e-17 75.9
MS.gene043709.t1 MTR_1g034210 37.895 95 59 0 13 107 7 101 4.11e-17 77.4
MS.gene043709.t1 MTR_1g034210 37.895 95 59 0 13 107 7 101 5.00e-17 77.4
MS.gene043709.t1 MTR_6g061070 33.835 133 83 3 7 139 12 139 9.09e-17 77.0
MS.gene043709.t1 MTR_3g005440 35.227 88 57 0 27 114 34 121 1.26e-16 76.6
MS.gene043709.t1 MTR_3g005440 33.673 98 65 0 17 114 246 343 9.75e-15 71.2
MS.gene043709.t1 MTR_1g035500 32.468 154 91 4 7 160 19 159 2.26e-16 73.2
MS.gene043709.t1 MTR_1g035510 34.884 129 69 4 28 156 1 114 3.05e-15 70.9
MS.gene043709.t1 MTR_1g035470 34.884 129 70 5 28 156 1 115 9.13e-15 67.8
MS.gene043709.t1 MTR_3g005420 28.788 132 84 3 13 135 21 151 2.00e-14 70.5
MS.gene043709.t1 MTR_1g035520 43.182 88 47 2 28 115 87 171 2.18e-14 70.1
MS.gene043709.t1 MTR_1g021440 33.673 98 64 1 6 103 16 112 3.78e-14 68.6
MS.gene043709.t1 MTR_1g021440 33.673 98 64 1 6 103 16 112 4.22e-14 68.6
MS.gene043709.t1 MTR_1g021440 33.673 98 64 1 6 103 16 112 6.27e-14 68.6
MS.gene043709.t1 MTR_1g021440 33.673 98 64 1 6 103 16 112 6.41e-14 68.6
MS.gene043709.t1 MTR_1g021410 28.105 153 97 2 14 157 23 171 6.97e-14 68.6
MS.gene043709.t1 MTR_1g021400 31.959 97 66 0 11 107 28 124 7.13e-14 68.6
MS.gene043709.t1 MTR_1g021360 36.905 84 53 0 24 107 193 276 2.17e-13 67.4
MS.gene043709.t1 MTR_1g021360 31.690 142 89 4 11 147 24 162 8.25e-13 65.5
MS.gene043709.t1 MTR_1g021320 28.859 149 97 2 11 153 23 168 2.22e-13 67.4
MS.gene043709.t1 MTR_1g021320 33.333 90 59 1 14 103 184 272 1.54e-12 65.1
MS.gene043709.t1 MTR_1g021350 31.915 94 64 0 14 107 22 115 4.89e-13 65.9
MS.gene043709.t1 MTR_1g021380 36.264 91 54 1 16 102 20 110 1.83e-12 64.3
MS.gene043709.t1 MTR_1g021435 31.633 98 67 0 6 103 15 112 2.10e-12 64.3
MS.gene043709.t1 MTR_1g021290 34.444 90 58 1 14 103 157 245 8.26e-12 62.4
MS.gene043709.t1 MTR_1g021270 34.146 82 54 0 27 108 29 110 4.02e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043709.t1 AT3G18990 43.434 99 56 0 13 111 6 104 5.67e-25 99.0
MS.gene043709.t1 AT4G01580 40.870 115 67 1 3 116 19 133 8.69e-25 95.1
MS.gene043709.t1 AT3G18990 43.434 99 56 0 13 111 6 104 9.05e-25 98.6
MS.gene043709.t1 AT1G49475 39.516 124 72 2 12 133 33 155 1.21e-24 94.7
MS.gene043709.t1 AT3G18960 39.130 115 69 1 3 116 19 133 4.66e-23 90.9
MS.gene043709.t1 AT3G18960 39.130 115 69 1 3 116 19 133 5.78e-23 90.9
MS.gene043709.t1 AT3G18960 44.318 88 49 0 29 116 9 96 9.99e-19 79.0
MS.gene043709.t1 AT3G53310 34.314 102 65 2 3 104 2 101 3.68e-13 65.9

Find 0 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTTTTCTTATAATA+TGG + 50841:6973-6992 MS.gene043709:intergenic 10.0%
!! AAAAAATTAAGGAAAGAAAA+TGG + 50841:6885-6904 MS.gene043709:intergenic 15.0%
!! AATAATAGAATCTAAGTAAA+AGG + 50841:6630-6649 MS.gene043709:intergenic 15.0%
!! TGAAAGAAAAGAAAAAATTA+AGG + 50841:6896-6915 MS.gene043709:intergenic 15.0%
!!! CATTTGAGTTTTATATTTAA+GGG - 50841:7007-7026 MS.gene043709:intron 15.0%
!! AGAAACATTCAAAAAACTAT+GGG - 50841:7292-7311 MS.gene043709:intron 20.0%
!! ATAACTATCATGTATATTTG+TGG + 50841:7179-7198 MS.gene043709:intergenic 20.0%
!! ATAAGAAAAAAAAATGCACA+TGG - 50841:6977-6996 MS.gene043709:intron 20.0%
!! TATACATGATAGTTATCTAT+CGG - 50841:7183-7202 MS.gene043709:intron 20.0%
!! TGAAAACTAAGTTATTATTG+TGG - 50841:7381-7400 MS.gene043709:intron 20.0%
!! TGCAAAATGGAAATTAAATT+TGG - 50841:7552-7571 MS.gene043709:intron 20.0%
!! TTAAATTTGGTAAATAGTGA+TGG - 50841:7565-7584 MS.gene043709:intron 20.0%
!! TTTCAACAAGTAATTAACTT+GGG - 50841:6911-6930 MS.gene043709:CDS 20.0%
!!! AATAACTTAGTTTTCAAATC+TGG + 50841:7377-7396 MS.gene043709:intergenic 20.0%
!!! GCAATTTTTTTTTTCTTCAA+AGG - 50841:7029-7048 MS.gene043709:intron 20.0%
!!! GCATTTGAGTTTTATATTTA+AGG - 50841:7006-7025 MS.gene043709:intron 20.0%
!!! TTTTTTCTTATAATATGGGA+CGG + 50841:6968-6987 MS.gene043709:intergenic 20.0%
! AAAAAGTTTGTGGAGAAATA+TGG - 50841:7487-7506 MS.gene043709:intron 25.0%
! ATATATCTAAAGACTCCAAA+TGG - 50841:7529-7548 MS.gene043709:intron 25.0%
! CAAGTATTATCTTGAAAAAG+CGG + 50841:6576-6595 MS.gene043709:intergenic 25.0%
! CTTTCAACAAGTAATTAACT+TGG - 50841:6910-6929 MS.gene043709:CDS 25.0%
! GAAAGGAAAATTTATCTCTA+AGG + 50841:7716-7735 MS.gene043709:intergenic 25.0%
! GAGAAACATTCAAAAAACTA+TGG - 50841:7291-7310 MS.gene043709:intron 25.0%
! TAAATAGTGATGGCAAAATA+TGG - 50841:7575-7594 MS.gene043709:intron 25.0%
! TCTATCATAAAACACATTAG+TGG + 50841:7084-7103 MS.gene043709:intergenic 25.0%
! TTATATCATGCATGCTAATT+TGG - 50841:7441-7460 MS.gene043709:intron 25.0%
!! AATTAACTTGGGTAAGATAT+TGG - 50841:6922-6941 MS.gene043709:CDS 25.0%
!! TGAAAAAACTTCTCTTTTTG+AGG - 50841:7669-7688 MS.gene043709:intron 25.0%
!!! CTATCGGATTTACTTTTTTT+GGG - 50841:7199-7218 MS.gene043709:intron 25.0%
!!! TATTTCTCCACAAACTTTTT+TGG + 50841:7487-7506 MS.gene043709:intergenic 25.0%
!!! TCTATCGGATTTACTTTTTT+TGG - 50841:7198-7217 MS.gene043709:intron 25.0%
!!! TTGAGTTAGATTCTTTTTTC+AGG - 50841:6665-6684 MS.gene043709:CDS 25.0%
AAATATGGTTTGAAAAGGGT+TGG - 50841:7590-7609 MS.gene043709:intron 30.0%
ACTACAAGATGTGTATTTAG+AGG - 50841:7243-7262 MS.gene043709:intron 30.0%
ATTATCTTGAAAAAGCGGAT+TGG + 50841:6571-6590 MS.gene043709:intergenic 30.0%
CTACAAGATGTGTATTTAGA+GGG - 50841:7244-7263 MS.gene043709:intron 30.0%
GATGATACCAAAAAAGTTTG+TGG - 50841:7477-7496 MS.gene043709:intron 30.0%
GGAAAGAAAATGGTTCAAAT+TGG + 50841:6875-6894 MS.gene043709:intergenic 30.0%
GGCAAAATATGGTTTGAAAA+GGG - 50841:7586-7605 MS.gene043709:intron 30.0%
GTTAAAAGTCTCACATTACT+TGG - 50841:7413-7432 MS.gene043709:intron 30.0%
TAGAGGGAATTAGTGATATT+GGG - 50841:7260-7279 MS.gene043709:intron 30.0%
TGAAGTTGAGAAATTGCAAA+AGG - 50841:7834-7853 MS.gene043709:CDS 30.0%
TGGCAAAATATGGTTTGAAA+AGG - 50841:7585-7604 MS.gene043709:intron 30.0%
TTAGAGGGAATTAGTGATAT+TGG - 50841:7259-7278 MS.gene043709:intron 30.0%
TTTCATCAACCATGTGAAAT+TGG - 50841:7796-7815 MS.gene043709:intron 30.0%
! AATTTCCATTTTGCACCATT+TGG + 50841:7547-7566 MS.gene043709:intergenic 30.0%
!! TGGTTGATGAAATTCAAAAG+AGG + 50841:7788-7807 MS.gene043709:intergenic 30.0%
AAGAATGTCACAAGACTTGT+TGG - 50841:6788-6807 MS.gene043709:CDS 35.0%
ACATTCAAAAAACTATGGGC+TGG - 50841:7296-7315 MS.gene043709:intron 35.0%
AGAGGGAATTAGTGATATTG+GGG - 50841:7261-7280 MS.gene043709:intron 35.0%
ATTTGTAGCCCAATGATACT+AGG - 50841:7116-7135 MS.gene043709:intron 35.0%
CTTGCTCAAAATCTTCATGA+AGG - 50841:6592-6611 MS.gene043709:CDS 35.0%
GTATCATTGGGCTACAAATT+TGG + 50841:7115-7134 MS.gene043709:intergenic 35.0%
TTACTCATCTATGTCGCTAA+TGG - 50841:6841-6860 MS.gene043709:CDS 35.0%
TTTCTTATAATATGGGACGG+AGG + 50841:6965-6984 MS.gene043709:intergenic 35.0%
TTTGTAGCCCAATGATACTA+GGG - 50841:7117-7136 MS.gene043709:intron 35.0%
TTTGTGGAGAAATATGGAGA+AGG - 50841:7493-7512 MS.gene043709:intron 35.0%
! ACTTGTTGGTCATAGTTTGT+GGG - 50841:6802-6821 MS.gene043709:CDS 35.0%
! CATATCTGAAAAGCACAAGA+TGG + 50841:7651-7670 MS.gene043709:intergenic 35.0%
!! ACTTAGTTTTCAAATCTGGC+CGG + 50841:7373-7392 MS.gene043709:intergenic 35.0%
!! TTTTTGGGAAAACGCACATT+CGG - 50841:7214-7233 MS.gene043709:intron 35.0%
AGAAACGCCCTAGTATCATT+GGG + 50841:7127-7146 MS.gene043709:intergenic 40.0%
AGACTCCAAATGGTGCAAAA+TGG - 50841:7539-7558 MS.gene043709:intron 40.0%
AGCAGCCTGTTTACTCAATT+CGG - 50841:7319-7338 MS.gene043709:intron 40.0%
CCATGTGAAATTGGAAGCAA+TGG - 50841:7805-7824 MS.gene043709:intron 40.0%
CCATTGCTTCCAATTTCACA+TGG + 50841:7808-7827 MS.gene043709:intergenic 40.0%
GAGGGAATTAGTGATATTGG+GGG - 50841:7262-7281 MS.gene043709:intron 40.0%
TCATTGGGCTACAAATTTGG+TGG + 50841:7112-7131 MS.gene043709:intergenic 40.0%
TGGTGCCTTAGAGATATTGA+GGG + 50841:7159-7178 MS.gene043709:intergenic 40.0%
! ATACTAGGGCGTTTCTCTTT+AGG - 50841:7131-7150 MS.gene043709:intron 40.0%
! GAGTATCATTCTCTAGCTCA+TGG - 50841:7625-7644 MS.gene043709:intron 40.0%
! TTAGCTGCAACTCTTTGGAA+AGG + 50841:7733-7752 MS.gene043709:intergenic 40.0%
! TTGGTCATAGTTTGTGGGTT+AGG - 50841:6807-6826 MS.gene043709:CDS 40.0%
!! GACTTGTTGGTCATAGTTTG+TGG - 50841:6801-6820 MS.gene043709:CDS 40.0%
!! TTGGCTTGGACAAGTTAGTT+CGG - 50841:7347-7366 MS.gene043709:intron 40.0%
CCAAAGAGTTGCAGCTAAAG+AGG - 50841:7735-7754 MS.gene043709:intron 45.0%
CCTCTTTAGCTGCAACTCTT+TGG + 50841:7738-7757 MS.gene043709:intergenic 45.0%
CTATGTCGCTAATGGCTTCA+TGG - 50841:6849-6868 MS.gene043709:CDS 45.0%
GAGAAACGCCCTAGTATCAT+TGG + 50841:7128-7147 MS.gene043709:intergenic 45.0%
GGTGCCTTAGAGATATTGAG+GGG + 50841:7158-7177 MS.gene043709:intergenic 45.0%
GTGCCTTAGAGATATTGAGG+GGG + 50841:7157-7176 MS.gene043709:intergenic 45.0%
GTGGTGCCTTAGAGATATTG+AGG + 50841:7160-7179 MS.gene043709:intergenic 45.0%
TTTACTCAATTCGGCCCACT+TGG - 50841:7328-7347 MS.gene043709:intron 45.0%
! ACTAACTTGTCCAAGCCAAG+TGG + 50841:7346-7365 MS.gene043709:intergenic 45.0%
! CTAACTTGTCCAAGCCAAGT+GGG + 50841:7345-7364 MS.gene043709:intergenic 45.0%
!! GTTGCAGCTAAAGAGGTATC+TGG - 50841:7742-7761 MS.gene043709:intron 45.0%
!!! ATTTTTTTTTCTTATAATAT+GGG + 50841:6972-6991 MS.gene043709:intergenic 5.0%
AGGCCCCCTCAATATCTCTA+AGG - 50841:7151-7170 MS.gene043709:intron 50.0%
CTTGGACAAGTTAGTTCGGC+CGG - 50841:7351-7370 MS.gene043709:intron 50.0%
GTGGGCCGAATTGAGTAAAC+AGG + 50841:7327-7346 MS.gene043709:intergenic 50.0%
CATGCTGACAGAGAGTCCAG+AGG - 50841:7871-7890 MS.gene043709:CDS 55.0%
TCAATTCGGCCCACTTGGCT+TGG - 50841:7333-7352 MS.gene043709:intron 55.0%


Chromosome Type Strat End Strand Name
50841 gene 6541 7906 6541 ID=MS.gene043709
50841 mRNA 6541 7906 6541 ID=MS.gene043709.t1;Parent=MS.gene043709
50841 exon 7826 7906 7826 ID=MS.gene043709.t1.exon1;Parent=MS.gene043709.t1
50841 CDS 7826 7906 7826 ID=cds.MS.gene043709.t1;Parent=MS.gene043709.t1
50841 exon 6541 6969 6541 ID=MS.gene043709.t1.exon2;Parent=MS.gene043709.t1
50841 CDS 6541 6969 6541 ID=cds.MS.gene043709.t1;Parent=MS.gene043709.t1
Gene Sequence

>MS.gene043709

ATGGCTAGTGAAACTAATGAGTTGAAGCCAATCCGCTTTTTCAAGATAATACTTGCTCAAAATCTTCATGAAGGAAAGCTTGTAAGCCTTTTACTTAGATTCTATTATTTTTGTTCTGATAATCTTGAGTTAGATTCTTTTTTCAGGTAGTTTATATTGTTTGTATATTTCATAACATGATTACATTATTTTGTGTTTTGCTTGCAACTTTTTATTATGTCACAAGATTTTTTTTTTATTTTTTTAAAAGAATGTCACAAGACTTGTTGGTCATAGTTTGTGGGTTAGGCATTATTACATTTACTCATCTATGTCGCTAATGGCTTCATGGCCAATTTGAACCATTTTCTTTCCTTAATTTTTTCTTTTCTTTCAACAAGTAATTAACTTGGGTAAGATATTGGACAAGAATGACATACTTCCTCCGTCCCATATTATAAGAAAAAAAAATGCACATGGTAAATTGCATTTGAGTTTTATATTTAAGGGCAATTTTTTTTTTCTTCAAAGGAGACTTGTAATTAAGAATTCAAGTTTAGTCCACTAATGTGTTTTATGATAGACTTGACCACCAAATTTGTAGCCCAATGATACTAGGGCGTTTCTCTTTAGGCCCCCTCAATATCTCTAAGGCACCACAAATATACATGATAGTTATCTATCGGATTTACTTTTTTTGGGAAAACGCACATTCGGTAGATAACTACAAGATGTGTATTTAGAGGGAATTAGTGATATTGGGGGCTTAAAGAGAAACATTCAAAAAACTATGGGCTGGAGCAGCCTGTTTACTCAATTCGGCCCACTTGGCTTGGACAAGTTAGTTCGGCCGGCCAGATTTGAAAACTAAGTTATTATTGTGGCAAATGTGAGTTAAAAGTCTCACATTACTTGGAAAAGTTATATCATGCATGCTAATTTGGTTTAATTATGACAGATGATACCAAAAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAATAGTGATGGCAAAATATGGTTTGAAAAGGGTTGGAAAGAATTTGCAGAGTATCATTCTCTAGCTCATGGCCATCTTGTGCTTTTCAGATATGAAAAAACTTCTCTTTTTGAGGTACATATCTTTGATAAGAGTGCCTTAGAGATAAATTTTCCTTTCCAAAGAGTTGCAGCTAAAGAGGTATCTGGTGAAAAGAGAAAAGCTAACTCCTCTTTTGAATTTCATCAACCATGTGAAATTGGAAGCAATGGTTGTGTTGAAGTTGAGAAATTGCAAAAGGTAGCAACTTTGCATCATGCTGACAGAGAGTCCAGAGGTATACAATTTTGT

Protein sequence

>MS.gene043709.t1

MASETNELKPIRFFKIILAQNLHEGKLMIPKKFVEKYGEGLSNAIYLKTPNGAKWKLNLVNSDGKIWFEKGWKEFAEYHSLAHGHLVLFRYEKTSLFEVHIFDKSALEINFPFQRVAAKEVSGEKRKANSSFEFHQPCEIGSNGCVEVEKLQKVATLHHADRESRGIQFC