Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043713.t1 | XP_003622663.1 | 80.6 | 196 | 37 | 1 | 2 | 197 | 121 | 315 | 1.00E-85 | 326.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043713.t1 | Q9XIB5 | 31.3 | 131 | 82 | 4 | 43 | 172 | 98 | 221 | 1.2e-07 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043713.t1 | G7KVV4 | 80.6 | 196 | 37 | 1 | 2 | 197 | 121 | 315 | 7.3e-86 | 326.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene043713.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043713.t1 | MTR_7g050560 | 80.612 | 196 | 37 | 1 | 2 | 197 | 121 | 315 | 7.45e-117 | 335 |
| MS.gene043713.t1 | MTR_7g050590 | 75.691 | 181 | 42 | 2 | 6 | 185 | 114 | 293 | 1.53e-97 | 285 |
| MS.gene043713.t1 | MTR_7g050580 | 63.731 | 193 | 55 | 2 | 5 | 197 | 138 | 315 | 2.29e-80 | 242 |
| MS.gene043713.t1 | MTR_7g050710 | 65.761 | 184 | 59 | 3 | 5 | 185 | 138 | 320 | 4.68e-77 | 234 |
| MS.gene043713.t1 | MTR_3g098790 | 63.536 | 181 | 58 | 3 | 6 | 185 | 139 | 312 | 2.59e-73 | 224 |
| MS.gene043713.t1 | MTR_7g050720 | 55.866 | 179 | 69 | 5 | 6 | 174 | 139 | 317 | 4.17e-58 | 185 |
| MS.gene043713.t1 | MTR_1g035460 | 40.541 | 111 | 55 | 3 | 62 | 172 | 188 | 287 | 6.13e-19 | 84.0 |
| MS.gene043713.t1 | MTR_1g108780 | 38.017 | 121 | 62 | 4 | 53 | 172 | 182 | 290 | 2.75e-17 | 79.3 |
| MS.gene043713.t1 | MTR_8g103940 | 34.483 | 174 | 89 | 7 | 12 | 172 | 170 | 331 | 2.21e-15 | 73.9 |
| MS.gene043713.t1 | MTR_4g068320 | 31.618 | 136 | 86 | 3 | 43 | 177 | 264 | 393 | 2.00e-12 | 65.5 |
| MS.gene043713.t1 | MTR_1g035490 | 36.364 | 121 | 70 | 4 | 54 | 172 | 81 | 196 | 9.45e-12 | 63.2 |
| MS.gene043713.t1 | MTR_1g034240 | 29.412 | 136 | 89 | 3 | 43 | 177 | 268 | 397 | 1.37e-11 | 62.8 |
| MS.gene043713.t1 | MTR_1g021330 | 34.234 | 111 | 69 | 3 | 64 | 173 | 255 | 362 | 5.57e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGGAGATGTTTATTTGC+AGG | 0.289134 | 7.4:-52640118 | MS.gene043713:CDS |
| TTGCATCAACCATGTGAAAT+TGG | 0.298517 | 7.4:-52641158 | MS.gene043713:CDS |
| CTACTCAGCATGACCCTTAC+AGG | 0.311216 | 7.4:-52640063 | MS.gene043713:CDS |
| AGGTGTTAAATGAAGGAGTT+TGG | 0.403488 | 7.4:-52640098 | MS.gene043713:CDS |
| CAGAATATACCACGTATGTT+TGG | 0.412403 | 7.4:-52640168 | MS.gene043713:CDS |
| GTCTAAAATGTGGTCCTGTA+AGG | 0.417430 | 7.4:+52640049 | None:intergenic |
| GAGAAGCTTCTTCTTTCAAT+CGG | 0.428772 | 7.4:+52639784 | None:intergenic |
| ATGCTGAGTAGGTACGTTGC+AGG | 0.447967 | 7.4:+52640075 | None:intergenic |
| GCAGCTAAAATGGTCTCTAA+TGG | 0.456051 | 7.4:-52641212 | None:intergenic |
| GAAGAAGCTTCTCAAGGATA+AGG | 0.472251 | 7.4:-52639774 | None:intergenic |
| TATTTGCAGGTGTTAAATGA+AGG | 0.472581 | 7.4:-52640105 | MS.gene043713:CDS |
| GAGGACAATAACTTGAAAGT+TGG | 0.475501 | 7.4:-52640000 | MS.gene043713:CDS |
| TGAAGTTGAGAAATTGCAAA+AGG | 0.487851 | 7.4:-52641120 | MS.gene043713:CDS |
| AGAAGCTTCTTCTTTCAATC+GGG | 0.491592 | 7.4:+52639785 | None:intergenic |
| CTCTCTTCTTATCCAACTCA+AGG | 0.506333 | 7.4:+52640136 | None:intergenic |
| TCTAAAGATGTGAACAATGA+AGG | 0.509907 | 7.4:+52639936 | None:intergenic |
| CTGTAAGGGTCATGCTGAGT+AGG | 0.540398 | 7.4:+52640064 | None:intergenic |
| CCATGTGAAATTGGAAGCAA+TGG | 0.548448 | 7.4:-52641149 | MS.gene043713:CDS |
| CCATTGCTTCCAATTTCACA+TGG | 0.551517 | 7.4:+52641149 | None:intergenic |
| TCTAAAATGTGGTCCTGTAA+GGG | 0.557222 | 7.4:+52640050 | None:intergenic |
| TAGACTCTCAGGTGGATGGA+AGG | 0.562253 | 7.4:-52640031 | MS.gene043713:CDS |
| TGCATGACAACAACAAAGGA+TGG | 0.566700 | 7.4:+52640706 | None:intergenic |
| TGGAACTTACCAGAACAAAA+CGG | 0.582937 | 7.4:+52640665 | None:intergenic |
| TGGTTGATGCAATTCAAAAG+AGG | 0.607778 | 7.4:+52641169 | None:intergenic |
| TTGAAAGAAGAAGCTTCTCA+AGG | 0.617350 | 7.4:-52639780 | MS.gene043713:CDS |
| TGGTAATAGACACCTTGAGT+TGG | 0.617534 | 7.4:-52640148 | MS.gene043713:CDS |
| CTTGAGTTGGATAAGAAGAG+AGG | 0.623819 | 7.4:-52640135 | MS.gene043713:CDS |
| CATGCTGACAGTGAGTCCAA+AGG | 0.661277 | 7.4:-52641083 | MS.gene043713:intron |
| CCACCTGAGAGTCTAAAATG+TGG | 0.688979 | 7.4:+52640039 | None:intergenic |
| TGCATGCATGACAACAACAA+AGG | 0.742589 | 7.4:+52640702 | None:intergenic |
| TGTCTATTACCAAACATACG+TGG | 0.746142 | 7.4:+52640159 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTCTTAAACATTTAAA+TGG | - | chr7.4:52640122-52640141 | MS.gene043713:CDS | 10.0% |
| !! | ATAAAATAAAAAAATGGAGA+AGG | + | chr7.4:52640106-52640125 | None:intergenic | 15.0% |
| !!! | ATTTGTCTTTTCTTTTTGTT+TGG | - | chr7.4:52640788-52640807 | MS.gene043713:intron | 20.0% |
| !!! | TAATACATATTTTCTGATCA+TGG | - | chr7.4:52640508-52640527 | MS.gene043713:intron | 20.0% |
| ! | ATCATCACATAGATGTTTAT+TGG | - | chr7.4:52640648-52640667 | MS.gene043713:intron | 25.0% |
| ! | ATGCATACATATTCTCTAAT+TGG | + | chr7.4:52640404-52640423 | None:intergenic | 25.0% |
| ! | TCTATGTGATGATATCATAA+TGG | + | chr7.4:52640641-52640660 | None:intergenic | 25.0% |
| ! | TGCAAAATTTGAACACAATA+AGG | + | chr7.4:52640184-52640203 | None:intergenic | 25.0% |
| ! | TGTTAGTAGTTAATTAGTAG+AGG | - | chr7.4:52640455-52640474 | MS.gene043713:intron | 25.0% |
| ! | TGTTCTACAATAACTTTGAA+GGG | + | chr7.4:52640047-52640066 | None:intergenic | 25.0% |
| !! | TTATTGTGTTCAAATTTTGC+AGG | - | chr7.4:52640183-52640202 | MS.gene043713:intron | 25.0% |
| !!! | GCTTTTAGAAAATAAGTTGA+TGG | - | chr7.4:52640349-52640368 | MS.gene043713:intron | 25.0% |
| AAGACACAATCTTATACCTT+TGG | + | chr7.4:52639915-52639934 | None:intergenic | 30.0% | |
| ATGGTCTTTACTAGATTAAG+GGG | - | chr7.4:52640368-52640387 | MS.gene043713:intron | 30.0% | |
| CTAAAAGCCAAATGTTTCTT+TGG | + | chr7.4:52640337-52640356 | None:intergenic | 30.0% | |
| CTGTTCTACAATAACTTTGA+AGG | + | chr7.4:52640048-52640067 | None:intergenic | 30.0% | |
| GAATATGTATGCATCAACTA+AGG | - | chr7.4:52640410-52640429 | MS.gene043713:intron | 30.0% | |
| GATGGTCTTTACTAGATTAA+GGG | - | chr7.4:52640367-52640386 | MS.gene043713:intron | 30.0% | |
| GTAAGTTCCAAAGAAACATT+TGG | - | chr7.4:52640327-52640346 | MS.gene043713:intron | 30.0% | |
| TATTTGCAGGTGTTAAATGA+AGG | - | chr7.4:52640874-52640893 | MS.gene043713:intron | 30.0% | |
| TCTAAAGATGTGAACAATGA+AGG | + | chr7.4:52641046-52641065 | None:intergenic | 30.0% | |
| TGAAGTTGAGAAATTGCAAA+AGG | - | chr7.4:52639859-52639878 | MS.gene043713:intron | 30.0% | |
| TGATGGTCTTTACTAGATTA+AGG | - | chr7.4:52640366-52640385 | MS.gene043713:intron | 30.0% | |
| AGAAGCTTCTTCTTTCAATC+GGG | + | chr7.4:52641197-52641216 | None:intergenic | 35.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | - | chr7.4:52640881-52640900 | MS.gene043713:intron | 35.0% | |
| AGTGTTCAACATCTCAAAGT+AGG | - | chr7.4:52641088-52641107 | MS.gene043713:CDS | 35.0% | |
| CAGAATATACCACGTATGTT+TGG | - | chr7.4:52640811-52640830 | MS.gene043713:intron | 35.0% | |
| GAGAAGCTTCTTCTTTCAAT+CGG | + | chr7.4:52641198-52641217 | None:intergenic | 35.0% | |
| GAGGACAATAACTTGAAAGT+TGG | - | chr7.4:52640979-52640998 | MS.gene043713:intron | 35.0% | |
| TATTTCCCATTGATACCAGT+TGG | - | chr7.4:52640564-52640583 | MS.gene043713:intron | 35.0% | |
| TGGAACTTACCAGAACAAAA+CGG | + | chr7.4:52640317-52640336 | None:intergenic | 35.0% | |
| TGTCTATTACCAAACATACG+TGG | + | chr7.4:52640823-52640842 | None:intergenic | 35.0% | |
| TTCGTTCAATGATGTATCGA+TGG | - | chr7.4:52640008-52640027 | MS.gene043713:CDS | 35.0% | |
| TTGAAAGAAGAAGCTTCTCA+AGG | - | chr7.4:52641199-52641218 | MS.gene043713:CDS | 35.0% | |
| TTGCATCAACCATGTGAAAT+TGG | - | chr7.4:52639821-52639840 | MS.gene043713:intron | 35.0% | |
| ! | TCTAAAATGTGGTCCTGTAA+GGG | + | chr7.4:52640932-52640951 | None:intergenic | 35.0% |
| ! | TGGTTGATGCAATTCAAAAG+AGG | + | chr7.4:52639813-52639832 | None:intergenic | 35.0% |
| AAGGACCAACTGGTATCAAT+GGG | + | chr7.4:52640572-52640591 | None:intergenic | 40.0% | |
| CCATGTGAAATTGGAAGCAA+TGG | - | chr7.4:52639830-52639849 | MS.gene043713:intron | 40.0% | |
| CCATTGCTTCCAATTTCACA+TGG | + | chr7.4:52639833-52639852 | None:intergenic | 40.0% | |
| CTCTCTTCTTATCCAACTCA+AGG | + | chr7.4:52640846-52640865 | None:intergenic | 40.0% | |
| GAGAGGAGATGTTTATTTGC+AGG | - | chr7.4:52640861-52640880 | MS.gene043713:intron | 40.0% | |
| TGCATGACAACAACAAAGGA+TGG | + | chr7.4:52640276-52640295 | None:intergenic | 40.0% | |
| TGCATGCATGACAACAACAA+AGG | + | chr7.4:52640280-52640299 | None:intergenic | 40.0% | |
| TGGTAATAGACACCTTGAGT+TGG | - | chr7.4:52640831-52640850 | MS.gene043713:intron | 40.0% | |
| ! | ATTTTAGACTCTCAGGTGGA+TGG | - | chr7.4:52640944-52640963 | MS.gene043713:intron | 40.0% |
| ! | GTCTAAAATGTGGTCCTGTA+AGG | + | chr7.4:52640933-52640952 | None:intergenic | 40.0% |
| !! | CGTTAAACACCGTTTTGTTC+TGG | - | chr7.4:52640305-52640324 | MS.gene043713:intron | 40.0% |
| !! | CTTGAGTTGGATAAGAAGAG+AGG | - | chr7.4:52640844-52640863 | MS.gene043713:intron | 40.0% |
| AAATTGCCCAGCTCAAATGG+AGG | - | chr7.4:52640601-52640620 | MS.gene043713:intron | 45.0% | |
| AATTGCCCAGCTCAAATGGA+GGG | - | chr7.4:52640602-52640621 | MS.gene043713:intron | 45.0% | |
| CAAGGACCAACTGGTATCAA+TGG | + | chr7.4:52640573-52640592 | None:intergenic | 45.0% | |
| CGAAAATTGCCCAGCTCAAA+TGG | - | chr7.4:52640598-52640617 | MS.gene043713:intron | 45.0% | |
| TAGAACCCTCCATTTGAGCT+GGG | + | chr7.4:52640610-52640629 | None:intergenic | 45.0% | |
| ! | CCACATTTTAGACTCTCAGG+TGG | - | chr7.4:52640940-52640959 | MS.gene043713:intron | 45.0% |
| ! | CCACCTGAGAGTCTAAAATG+TGG | + | chr7.4:52640943-52640962 | None:intergenic | 45.0% |
| ! | GGACCACATTTTAGACTCTC+AGG | - | chr7.4:52640937-52640956 | MS.gene043713:intron | 45.0% |
| ! | TGGGCAATTTTCGCTCAACA+AGG | + | chr7.4:52640591-52640610 | None:intergenic | 45.0% |
| !!! | TGGATGGAAGGCATTTTCAG+AGG | - | chr7.4:52640960-52640979 | MS.gene043713:intron | 45.0% |
| !! | TAAGAAATAAAATAAAAAAA+TGG | + | chr7.4:52640112-52640131 | None:intergenic | 5.0% |
| !! | TATATATTATAATAACAAAA+TGG | - | chr7.4:52640683-52640702 | MS.gene043713:CDS | 5.0% |
| ATGCTGAGTAGGTACGTTGC+AGG | + | chr7.4:52640907-52640926 | None:intergenic | 50.0% | |
| CATGCTGACAGTGAGTCCAA+AGG | - | chr7.4:52639896-52639915 | MS.gene043713:CDS | 50.0% | |
| CTACTCAGCATGACCCTTAC+AGG | - | chr7.4:52640916-52640935 | MS.gene043713:intron | 50.0% | |
| CTAGAACCCTCCATTTGAGC+TGG | + | chr7.4:52640611-52640630 | None:intergenic | 50.0% | |
| CTGTAAGGGTCATGCTGAGT+AGG | + | chr7.4:52640918-52640937 | None:intergenic | 50.0% | |
| TAGACTCTCAGGTGGATGGA+AGG | - | chr7.4:52640948-52640967 | MS.gene043713:intron | 50.0% | |
| TTCGCTCAACAAGGACCAAC+TGG | + | chr7.4:52640582-52640601 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 52639776 | 52641225 | 52639776 | ID=MS.gene043713 |
| chr7.4 | mRNA | 52639776 | 52641225 | 52639776 | ID=MS.gene043713.t1;Parent=MS.gene043713 |
| chr7.4 | exon | 52641084 | 52641225 | 52641084 | ID=MS.gene043713.t1.exon1;Parent=MS.gene043713.t1 |
| chr7.4 | CDS | 52641084 | 52641225 | 52641084 | ID=cds.MS.gene043713.t1;Parent=MS.gene043713.t1 |
| chr7.4 | exon | 52640675 | 52640796 | 52640675 | ID=MS.gene043713.t1.exon2;Parent=MS.gene043713.t1 |
| chr7.4 | CDS | 52640675 | 52640796 | 52640675 | ID=cds.MS.gene043713.t1;Parent=MS.gene043713.t1 |
| chr7.4 | exon | 52639896 | 52640187 | 52639896 | ID=MS.gene043713.t1.exon3;Parent=MS.gene043713.t1 |
| chr7.4 | CDS | 52639896 | 52640187 | 52639896 | ID=cds.MS.gene043713.t1;Parent=MS.gene043713.t1 |
| chr7.4 | exon | 52639776 | 52639813 | 52639776 | ID=MS.gene043713.t1.exon4;Parent=MS.gene043713.t1 |
| chr7.4 | CDS | 52639776 | 52639813 | 52639776 | ID=cds.MS.gene043713.t1;Parent=MS.gene043713.t1 |
| Gene Sequence |
| Protein sequence |
