AlfalfaGEDB | MS.gene043718

M. sativa cultivar XinJiangDaYe / MS.gene043718

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043718.t1	RHN45637.1	90.6	139	13	0	1	139	1	139	3.70E-66	260.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043718.t1	Q9ZSH7	47.7	88	46	0	2	89	46	133	1.0e-16	87.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043718.t1	G7KVV4	90.6	139	13	0	1	139	28	166	2.7e-66	260.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene043718.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043718.t1	MTR_7g050560	90.647	139	13	0	1	139	28	166	1.91e-89	262
MS.gene043718.t1	MTR_7g050590	74.839	155	22	3	1	139	1	154	3.93e-75	225
MS.gene043718.t1	MTR_7g050720	67.308	156	34	1	1	139	25	180	4.44e-68	208
MS.gene043718.t1	MTR_7g050710	63.636	143	38	1	2	130	29	171	5.54e-57	180
MS.gene043718.t1	MTR_3g098790	62.745	153	36	3	1	139	28	173	9.20e-57	179
MS.gene043718.t1	MTR_7g050580	63.971	136	28	3	1	122	28	156	3.14e-50	162
MS.gene043718.t1	MTR_7g050640	72.093	86	24	0	1	86	28	113	9.91e-40	129
MS.gene043718.t1	MTR_1g035460	46.296	108	58	0	1	108	1	108	8.08e-31	114
MS.gene043718.t1	MTR_8g103940	51.807	83	40	0	2	84	55	137	7.29e-27	104
MS.gene043718.t1	MTR_1g108780	50.602	83	41	0	2	84	13	95	5.66e-26	101
MS.gene043718.t1	MTR_3g061330	53.012	83	39	0	2	84	26	108	2.23e-25	97.1
MS.gene043718.t1	MTR_3g061290	53.012	83	39	0	2	84	26	108	2.87e-25	95.5
MS.gene043718.t1	MTR_3g061370	44.860	107	59	0	2	108	23	129	5.48e-25	96.7
MS.gene043718.t1	MTR_5g044670	47.619	84	44	0	1	84	31	114	1.26e-24	96.7
MS.gene043718.t1	MTR_3g061320	53.012	83	39	0	2	84	26	108	5.37e-24	94.0
MS.gene043718.t1	MTR_3g061310	50.602	83	41	0	2	84	26	108	1.34e-23	93.2
MS.gene043718.t1	MTR_3g061350	41.121	107	63	0	2	108	26	132	3.74e-23	91.7
MS.gene043718.t1	MTR_1g035640	51.807	83	38	1	2	84	21	101	1.02e-22	92.8
MS.gene043718.t1	MTR_3g061340	48.810	84	42	1	2	84	26	109	2.20e-21	87.0
MS.gene043718.t1	MTR_3g005480	33.636	110	64	2	2	111	37	137	6.41e-21	85.5
MS.gene043718.t1	MTR_1g035570	46.988	83	42	1	2	84	21	101	1.16e-20	86.7
MS.gene043718.t1	MTR_4g068320	44.944	89	47	1	2	88	22	110	1.24e-20	86.7
MS.gene043718.t1	MTR_1g035480	39.231	130	65	4	1	130	32	147	2.07e-20	82.4
MS.gene043718.t1	MTR_3g005460	37.113	97	57	1	2	98	41	133	1.76e-19	83.6
MS.gene043718.t1	MTR_3g005460	35.714	98	62	1	2	98	278	375	5.33e-18	79.3
MS.gene043718.t1	MTR_5g070440	35.577	104	63	1	2	105	37	136	2.30e-19	81.6
MS.gene043718.t1	MTR_3g005490	35.789	95	61	0	2	96	37	131	2.66e-19	81.3
MS.gene043718.t1	MTR_3g005440	33.945	109	67	1	2	105	36	144	3.67e-19	82.4
MS.gene043718.t1	MTR_3g005440	37.234	94	59	0	5	98	261	354	2.46e-15	71.6
MS.gene043718.t1	MTR_3g005495	37.719	114	61	3	2	106	29	141	7.16e-19	81.6
MS.gene043718.t1	MTR_3g005495	39.175	97	59	0	2	98	259	355	8.96e-18	78.6
MS.gene043718.t1	MTR_1g050845	46.988	83	42	1	2	84	25	105	7.81e-19	82.0
MS.gene043718.t1	MTR_1g035510	38.462	130	64	4	1	130	1	114	8.39e-19	79.3
MS.gene043718.t1	MTR_1g035500	37.313	134	70	4	1	134	40	159	2.33e-18	77.4
MS.gene043718.t1	MTR_3g061220	38.318	107	51	2	2	108	26	117	3.36e-18	75.1
MS.gene043718.t1	MTR_1g034240	43.373	83	47	0	2	84	23	105	4.36e-18	79.3
MS.gene043718.t1	MTR_1g035620	44.578	83	44	1	2	84	25	105	2.12e-17	77.4
MS.gene043718.t1	MTR_1g035520	38.983	118	69	2	1	118	87	201	2.53e-17	77.0
MS.gene043718.t1	MTR_1g035470	37.692	130	66	5	1	130	1	115	6.09e-17	72.4
MS.gene043718.t1	MTR_3g005420	35.398	113	68	1	2	109	305	417	9.48e-17	75.9
MS.gene043718.t1	MTR_3g005420	29.565	115	74	2	2	109	56	170	1.98e-14	69.3
MS.gene043718.t1	MTR_1g033740	34.615	130	70	4	1	130	37	151	1.28e-16	72.8
MS.gene043718.t1	MTR_6g043890	36.607	112	63	2	2	105	31	142	1.76e-16	74.7
MS.gene043718.t1	MTR_6g061070	36.567	134	74	5	1	130	33	159	9.80e-16	72.8
MS.gene043718.t1	MTR_1g034210	39.759	83	48	1	2	84	23	103	7.39e-15	70.1
MS.gene043718.t1	MTR_1g034210	39.759	83	48	1	2	84	23	103	9.51e-15	69.7
MS.gene043718.t1	MTR_4g119990	40.000	90	53	1	2	90	31	120	1.04e-14	69.7
MS.gene043718.t1	MTR_1g035650	40.964	83	47	1	2	84	25	105	1.60e-14	67.8
MS.gene043718.t1	MTR_3g005420	29.565	115	74	2	2	109	37	151	2.42e-14	68.9
MS.gene043718.t1	MTR_1g021320	28.261	138	90	2	2	130	198	335	4.65e-14	68.2
MS.gene043718.t1	MTR_1g021320	32.258	124	77	2	2	119	41	163	6.18e-12	62.0
MS.gene043718.t1	MTR_1g021400	29.600	125	84	2	2	122	46	170	2.20e-12	63.2
MS.gene043718.t1	MTR_1g021290	31.313	99	65	1	2	97	171	269	3.13e-12	62.8
MS.gene043718.t1	MTR_1g021270	35.000	80	52	0	2	81	31	110	5.69e-12	62.0
MS.gene043718.t1	MTR_1g021440	37.333	75	47	0	2	76	38	112	7.81e-12	61.2
MS.gene043718.t1	MTR_1g021360	33.600	125	74	3	2	119	41	163	7.90e-12	61.6
MS.gene043718.t1	MTR_1g021360	35.443	79	51	0	2	80	198	276	3.53e-11	59.7
MS.gene043718.t1	MTR_1g021440	37.333	75	47	0	2	76	38	112	8.01e-12	61.2
MS.gene043718.t1	MTR_1g021440	37.333	75	47	0	2	76	38	112	1.05e-11	61.2
MS.gene043718.t1	MTR_1g021440	37.333	75	47	0	2	76	38	112	1.08e-11	61.2
MS.gene043718.t1	MTR_1g021410	27.612	134	93	2	2	131	38	171	1.10e-11	61.2
MS.gene043718.t1	MTR_1g035440	36.782	87	54	1	2	87	31	117	2.22e-11	59.7
MS.gene043718.t1	MTR_1g021380	37.333	75	47	0	2	76	37	111	3.55e-11	59.7
MS.gene043718.t1	MTR_1g021435	34.667	75	49	0	2	76	38	112	8.46e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene043718.t1	AT4G01580	47.727	88	46	0	2	89	46	133	1.16e-22	88.6
MS.gene043718.t1	AT3G18960	46.591	88	47	0	2	89	46	133	1.97e-21	85.5
MS.gene043718.t1	AT3G18960	46.591	88	47	0	2	89	9	96	2.31e-21	84.7
MS.gene043718.t1	AT3G18960	46.591	88	47	0	2	89	46	133	2.39e-21	85.5
MS.gene043718.t1	AT1G49475	46.512	86	45	1	2	87	50	134	2.46e-21	85.1
MS.gene043718.t1	AT3G18990	48.193	83	43	0	2	84	22	104	5.77e-21	87.0
MS.gene043718.t1	AT3G18990	48.193	83	43	0	2	84	22	104	7.04e-21	87.0

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAAAATGGAAATTAAATT+TGG	0.194062	7.4:-52732491	MS.gene043718:CDS
TATTTCTCCACAAACTTTCT+TGG	0.217927	7.4:+52732559	None:intergenic
TTTCATCAGCCACATGAAAT+TGG	0.246620	7.4:-52732244	MS.gene043718:CDS
CCAAAGAGTTTCAGCCAAAA+AGG	0.275595	7.4:-52732308	MS.gene043718:CDS
AGAAAGTTTGTGGAGAAATA+TGG	0.293086	7.4:-52732556	MS.gene043718:CDS
TCTAGCTCATGGCCATCTTC+TGG	0.301077	7.4:-52732407	MS.gene043718:CDS
GAATGGATAATTTATCTCTA+AGG	0.305393	7.4:+52732330	None:intergenic
AGACTCCAAATGGTGCAAAA+TGG	0.337543	7.4:-52732504	MS.gene043718:CDS
TGAAGTTCAGAAATTGAATA+AGG	0.355428	7.4:-52732206	MS.gene043718:CDS
TAAAGAGTGATGGCAAGATA+TGG	0.424057	7.4:-52732468	MS.gene043718:CDS
GATGATGCCAAGAAAGTTTG+TGG	0.440968	7.4:-52732566	MS.gene043718:CDS
GATATTTGAAAACCAGAAGA+TGG	0.469802	7.4:+52732395	None:intergenic
AGATATGGTTTGAGAAGGGT+TGG	0.481735	7.4:-52732453	MS.gene043718:CDS
ATATATCTAAAGACTCCAAA+TGG	0.494837	7.4:-52732514	MS.gene043718:CDS
TTGGCTGAAACTCTTTGGAA+TGG	0.508092	7.4:+52732313	None:intergenic
TTAAATTTGGTAAAGAGTGA+TGG	0.510387	7.4:-52732478	MS.gene043718:CDS
TGGCAAGATATGGTTTGAGA+AGG	0.555350	7.4:-52732458	MS.gene043718:CDS
AGGCCTCCAATGGTCAAAAG+AGG	0.566812	7.4:-52732288	MS.gene043718:CDS
GAGTATCACTCTCTAGCTCA+TGG	0.575939	7.4:-52732418	MS.gene043718:CDS
TTTGTGGAGAAATATGGAGA+AGG	0.578281	7.4:-52732550	MS.gene043718:CDS
TGGCTGATGAAATTCAAAAG+AGG	0.587827	7.4:+52732255	None:intergenic
CTATTGCTTCCAATTTCATG+TGG	0.591075	7.4:+52732235	None:intergenic
GTAGCAACTTTGCATCATGC+TGG	0.604292	7.4:-52732184	MS.gene043718:CDS
GGCAAGATATGGTTTGAGAA+GGG	0.606783	7.4:-52732457	MS.gene043718:CDS
CATGCTGGCAGAGAGTCCAA+AGG	0.714363	7.4:-52732169	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGCAAAATGGAAATTAAATT+TGG	-	chr7.4:52732244-52732263	MS.gene043718:CDS	20.0%
!	ATATATCTAAAGACTCCAAA+TGG	-	chr7.4:52732221-52732240	MS.gene043718:CDS	25.0%
!	GAATGGATAATTTATCTCTA+AGG	+	chr7.4:52732408-52732427	None:intergenic	25.0%
!	TGAAGTTCAGAAATTGAATA+AGG	-	chr7.4:52732529-52732548	MS.gene043718:CDS	25.0%
!	TTAAATTTGGTAAAGAGTGA+TGG	-	chr7.4:52732257-52732276	MS.gene043718:CDS	25.0%
	AGAAAGTTTGTGGAGAAATA+TGG	-	chr7.4:52732179-52732198	MS.gene043718:CDS	30.0%
	GATATTTGAAAACCAGAAGA+TGG	+	chr7.4:52732343-52732362	None:intergenic	30.0%
	TATTTCTCCACAAACTTTCT+TGG	+	chr7.4:52732179-52732198	None:intergenic	30.0%
!	AATTTCCATTTTGCACCATT+TGG	+	chr7.4:52732239-52732258	None:intergenic	30.0%
	CTATTGCTTCCAATTTCATG+TGG	+	chr7.4:52732503-52732522	None:intergenic	35.0%
	TAAAGAGTGATGGCAAGATA+TGG	-	chr7.4:52732267-52732286	MS.gene043718:CDS	35.0%
	TGGCTGATGAAATTCAAAAG+AGG	+	chr7.4:52732483-52732502	None:intergenic	35.0%
	TTTCATCAGCCACATGAAAT+TGG	-	chr7.4:52732491-52732510	MS.gene043718:CDS	35.0%
	TTTGTGGAGAAATATGGAGA+AGG	-	chr7.4:52732185-52732204	MS.gene043718:CDS	35.0%
!	ATCTTTCCTCTTTTGACCAT+TGG	+	chr7.4:52732456-52732475	None:intergenic	35.0%
	AGACTCCAAATGGTGCAAAA+TGG	-	chr7.4:52732231-52732250	MS.gene043718:CDS	40.0%
	AGATATGGTTTGAGAAGGGT+TGG	-	chr7.4:52732282-52732301	MS.gene043718:CDS	40.0%
	CCAAAGAGTTTCAGCCAAAA+AGG	-	chr7.4:52732427-52732446	MS.gene043718:CDS	40.0%
	GGCAAGATATGGTTTGAGAA+GGG	-	chr7.4:52732278-52732297	MS.gene043718:CDS	40.0%
	TGGCAAGATATGGTTTGAGA+AGG	-	chr7.4:52732277-52732296	MS.gene043718:CDS	40.0%
!	TTGGCTGAAACTCTTTGGAA+TGG	+	chr7.4:52732425-52732444	None:intergenic	40.0%
!	TTTCCTCTTTTGACCATTGG+AGG	+	chr7.4:52732453-52732472	None:intergenic	40.0%
!!!	CCTTTTTGGCTGAAACTCTT+TGG	+	chr7.4:52732430-52732449	None:intergenic	40.0%
	GTAGCAACTTTGCATCATGC+TGG	-	chr7.4:52732551-52732570	MS.gene043718:CDS	45.0%
!	GAGTATCACTCTCTAGCTCA+TGG	-	chr7.4:52732317-52732336	MS.gene043718:CDS	45.0%
!	TTGACCATTGGAGGCCTTTT+TGG	+	chr7.4:52732444-52732463	None:intergenic	45.0%
	AGGCCTCCAATGGTCAAAAG+AGG	-	chr7.4:52732447-52732466	MS.gene043718:CDS	50.0%
	TCAGCCAAAAAGGCCTCCAA+TGG	-	chr7.4:52732437-52732456	MS.gene043718:CDS	50.0%
!	TCTAGCTCATGGCCATCTTC+TGG	-	chr7.4:52732328-52732347	MS.gene043718:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	52732170	52732587	52732170	ID=MS.gene043718
chr7.4	mRNA	52732170	52732587	52732170	ID=MS.gene043718.t1;Parent=MS.gene043718
chr7.4	exon	52732170	52732587	52732170	ID=MS.gene043718.t1.exon1;Parent=MS.gene043718.t1
chr7.4	CDS	52732170	52732587	52732170	ID=cds.MS.gene043718.t1;Parent=MS.gene043718.t1

Gene Sequence

>MS.gene043718

ATGATGCCAAGAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCGAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAAGAGTGATGGCAAGATATGGTTTGAGAAGGGTTGGAAAGAATTTGCAGAGTATCACTCTCTAGCTCATGGCCATCTTCTGGTTTTCAAATATCTAAGAAATTCTCATTTTCGTGTGCGCATCTTTGAAAAGAGTGCCTTAGAGATAAATTATCCATTCCAAAGAGTTTCAGCCAAAAAGGCCTCCAATGGTCAAAAGAGGAAAGATAACTCCTCTTTTGAATTTCATCAGCCACATGAAATTGGAAGCAATAGTTGTGTTGAAGTTCAGAAATTGAATAAGGTAGCAACTTTGCATCATGCTGGCAGAGAGTCCAAAG

Protein sequence

>MS.gene043718.t1

MMPRKFVEKYGEGLSNAIYLKTPNGAKWKLNLVKSDGKIWFEKGWKEFAEYHSLAHGHLLVFKYLRNSHFRVRIFEKSALEINYPFQRVSAKKASNGQKRKDNSSFEFHQPHEIGSNSCVEVQKLNKVATLHHAGRESK