Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04373.t1 | AES70699.1 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 1.30E-65 | 258.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04373.t1 | Q851V5 | 46.3 | 67 | 30 | 2 | 58 | 122 | 963 | 1025 | 4.2e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04373.t1 | G8A2Z9 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 9.2e-66 | 258.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04373.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04373.t1 | MTR_3g061300 | 96.825 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 1.50e-90 | 258 |
| MS.gene04373.t1 | MTR_3g061340 | 76.860 | 121 | 28 | 0 | 2 | 122 | 153 | 273 | 7.65e-64 | 196 |
| MS.gene04373.t1 | MTR_3g061370 | 70.248 | 121 | 36 | 0 | 2 | 122 | 160 | 280 | 1.16e-54 | 172 |
| MS.gene04373.t1 | MTR_3g061350 | 69.421 | 121 | 37 | 0 | 2 | 122 | 163 | 283 | 3.09e-53 | 169 |
| MS.gene04373.t1 | MTR_3g061320 | 72.321 | 112 | 31 | 0 | 11 | 122 | 167 | 278 | 5.13e-51 | 163 |
| MS.gene04373.t1 | MTR_3g061310 | 61.789 | 123 | 45 | 1 | 2 | 122 | 162 | 284 | 7.36e-43 | 142 |
| MS.gene04373.t1 | MTR_3g061330 | 65.556 | 90 | 30 | 1 | 2 | 90 | 162 | 251 | 7.97e-34 | 118 |
| MS.gene04373.t1 | MTR_8g103940 | 39.130 | 115 | 60 | 4 | 14 | 122 | 383 | 493 | 2.73e-17 | 77.0 |
| MS.gene04373.t1 | MTR_1g108780 | 38.938 | 113 | 64 | 3 | 14 | 122 | 345 | 456 | 1.76e-15 | 71.6 |
| MS.gene04373.t1 | MTR_1g108780 | 31.532 | 111 | 62 | 5 | 22 | 127 | 192 | 293 | 3.52e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04373.t1 | AT1G49480 | 33.945 | 109 | 62 | 4 | 17 | 120 | 114 | 217 | 1.44e-12 | 62.4 |
| MS.gene04373.t1 | AT1G49480 | 33.945 | 109 | 62 | 4 | 17 | 120 | 114 | 217 | 1.44e-12 | 62.4 |
| MS.gene04373.t1 | AT1G49480 | 33.945 | 109 | 62 | 4 | 17 | 120 | 114 | 217 | 1.44e-12 | 62.4 |
| MS.gene04373.t1 | AT3G18990 | 33.945 | 109 | 62 | 4 | 17 | 120 | 229 | 332 | 1.37e-11 | 60.5 |
| MS.gene04373.t1 | AT3G18990 | 33.945 | 109 | 62 | 4 | 17 | 120 | 229 | 332 | 1.38e-11 | 60.5 |
Find 30 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGTGTACCAACTTTAAA+TGG | 0.174261 | 3.4:+56955393 | MS.gene04373:intron |
| GATACATTAACGGTCACTTT+TGG | 0.356442 | 3.4:-56955625 | None:intergenic |
| ACTGATAAACAAGAAAGATT+TGG | 0.358474 | 3.4:+56955584 | MS.gene04373:CDS |
| GACCACTTTCTCTTGCAAAC+AGG | 0.362637 | 3.4:-56955533 | None:intergenic |
| CTACTCACCTAGCAGAAAAT+CGG | 0.374095 | 3.4:+56955267 | MS.gene04373:CDS |
| AAAGAACTACATCATGGAAT+TGG | 0.395138 | 3.4:+56955168 | MS.gene04373:CDS |
| ACATTTCTAGCCTCACAATA+AGG | 0.400255 | 3.4:-56955486 | None:intergenic |
| TCCTGAGCTCTAGCTCCCTT+TGG | 0.408256 | 3.4:-56955191 | None:intergenic |
| TCAATAACCCCATTTAAAGT+TGG | 0.446011 | 3.4:-56955402 | None:intergenic |
| AGTGTGTACCAACTTTAAAT+GGG | 0.455268 | 3.4:+56955394 | MS.gene04373:intron |
| TCTTGCAAACAGGGACCAAC+CGG | 0.463890 | 3.4:-56955523 | None:intergenic |
| AATGTTCGCCGCCTCTCGGC+CGG | 0.489655 | 3.4:+56955504 | MS.gene04373:CDS |
| AGTATGTGATGCTGCAGATT+GGG | 0.492313 | 3.4:+56955436 | MS.gene04373:CDS |
| ACCACTTTCTCTTGCAAACA+GGG | 0.504654 | 3.4:-56955532 | None:intergenic |
| TAGAAATGTTCGCCGCCTCT+CGG | 0.526048 | 3.4:+56955500 | MS.gene04373:CDS |
| ACATCATGGAATTGGCCAAA+GGG | 0.541191 | 3.4:+56955176 | MS.gene04373:CDS |
| AGAGAAAGTGGTCTGCAAGT+AGG | 0.546476 | 3.4:+56955543 | MS.gene04373:CDS |
| TACTCACCTAGCAGAAAATC+GGG | 0.546681 | 3.4:+56955268 | MS.gene04373:CDS |
| TCCCTGTTTGCAAGAGAAAG+TGG | 0.549015 | 3.4:+56955531 | MS.gene04373:CDS |
| GCCAAAGGGAGCTAGAGCTC+AGG | 0.549833 | 3.4:+56955190 | MS.gene04373:CDS |
| TTCGCCGCCTCTCGGCCGGT+TGG | 0.551560 | 3.4:+56955508 | MS.gene04373:CDS |
| TGCAGATTGGGAAAAGATCA+TGG | 0.571416 | 3.4:+56955448 | MS.gene04373:CDS |
| CTAATCAAAGAACTACATCA+TGG | 0.573456 | 3.4:+56955162 | MS.gene04373:CDS |
| AAGTATGTGATGCTGCAGAT+TGG | 0.577298 | 3.4:+56955435 | MS.gene04373:CDS |
| GGGACCAACCGGCCGAGAGG+CGG | 0.587468 | 3.4:-56955512 | None:intergenic |
| TACATCATGGAATTGGCCAA+AGG | 0.610272 | 3.4:+56955175 | MS.gene04373:CDS |
| GAAACTGCTTCCTTATTGTG+AGG | 0.617392 | 3.4:+56955476 | MS.gene04373:CDS |
| ACAGGGACCAACCGGCCGAG+AGG | 0.636650 | 3.4:-56955515 | None:intergenic |
| GTGTGTACCAACTTTAAATG+GGG | 0.652978 | 3.4:+56955395 | MS.gene04373:intron |
| TCACCTAGCAGAAAATCGGG+TGG | 0.717171 | 3.4:+56955271 | MS.gene04373:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATGAGAATTGTGAAAAA+AGG | - | chr3.4:56955245-56955264 | None:intergenic | 20.0% |
| ! | ACTGATAAACAAGAAAGATT+TGG | + | chr3.4:56955584-56955603 | MS.gene04373:CDS | 25.0% |
| AAAGAACTACATCATGGAAT+TGG | + | chr3.4:56955168-56955187 | MS.gene04373:CDS | 30.0% | |
| AGTGTGTACCAACTTTAAAT+GGG | + | chr3.4:56955394-56955413 | MS.gene04373:intron | 30.0% | |
| CTAATCAAAGAACTACATCA+TGG | + | chr3.4:56955162-56955181 | MS.gene04373:CDS | 30.0% | |
| GAGAATTGTGAAAAAAGGAT+TGG | - | chr3.4:56955240-56955259 | None:intergenic | 30.0% | |
| ! | TCAATAACCCCATTTAAAGT+TGG | - | chr3.4:56955405-56955424 | None:intergenic | 30.0% |
| CAGTGTGTACCAACTTTAAA+TGG | + | chr3.4:56955393-56955412 | MS.gene04373:intron | 35.0% | |
| GTGTGTACCAACTTTAAATG+GGG | + | chr3.4:56955395-56955414 | MS.gene04373:intron | 35.0% | |
| !! | ACATTTCTAGCCTCACAATA+AGG | - | chr3.4:56955489-56955508 | None:intergenic | 35.0% |
| AAGTATGTGATGCTGCAGAT+TGG | + | chr3.4:56955435-56955454 | MS.gene04373:CDS | 40.0% | |
| ACATCATGGAATTGGCCAAA+GGG | + | chr3.4:56955176-56955195 | MS.gene04373:CDS | 40.0% | |
| ACCACTTTCTCTTGCAAACA+GGG | - | chr3.4:56955535-56955554 | None:intergenic | 40.0% | |
| AGTATGTGATGCTGCAGATT+GGG | + | chr3.4:56955436-56955455 | MS.gene04373:CDS | 40.0% | |
| CTACTCACCTAGCAGAAAAT+CGG | + | chr3.4:56955267-56955286 | MS.gene04373:CDS | 40.0% | |
| GAAACTGCTTCCTTATTGTG+AGG | + | chr3.4:56955476-56955495 | MS.gene04373:CDS | 40.0% | |
| TACATCATGGAATTGGCCAA+AGG | + | chr3.4:56955175-56955194 | MS.gene04373:CDS | 40.0% | |
| TACTCACCTAGCAGAAAATC+GGG | + | chr3.4:56955268-56955287 | MS.gene04373:CDS | 40.0% | |
| TGCAGATTGGGAAAAGATCA+TGG | + | chr3.4:56955448-56955467 | MS.gene04373:CDS | 40.0% | |
| ! | GATTTTCTGCTAGGTGAGTA+GGG | - | chr3.4:56955268-56955287 | None:intergenic | 40.0% |
| AGAGAAAGTGGTCTGCAAGT+AGG | + | chr3.4:56955543-56955562 | MS.gene04373:CDS | 45.0% | |
| GACCACTTTCTCTTGCAAAC+AGG | - | chr3.4:56955536-56955555 | None:intergenic | 45.0% | |
| TCCCTGTTTGCAAGAGAAAG+TGG | + | chr3.4:56955531-56955550 | MS.gene04373:CDS | 45.0% | |
| ! | CGATTTTCTGCTAGGTGAGT+AGG | - | chr3.4:56955269-56955288 | None:intergenic | 45.0% |
| ! | TTACCACCCGATTTTCTGCT+AGG | - | chr3.4:56955277-56955296 | None:intergenic | 45.0% |
| TAGAAATGTTCGCCGCCTCT+CGG | + | chr3.4:56955500-56955519 | MS.gene04373:CDS | 50.0% | |
| TCACCTAGCAGAAAATCGGG+TGG | + | chr3.4:56955271-56955290 | MS.gene04373:CDS | 50.0% | |
| TCTTGCAAACAGGGACCAAC+CGG | - | chr3.4:56955526-56955545 | None:intergenic | 50.0% | |
| ! | TCCTGAGCTCTAGCTCCCTT+TGG | - | chr3.4:56955194-56955213 | None:intergenic | 55.0% |
| GCCAAAGGGAGCTAGAGCTC+AGG | + | chr3.4:56955190-56955209 | MS.gene04373:CDS | 60.0% | |
| AATGTTCGCCGCCTCTCGGC+CGG | + | chr3.4:56955504-56955523 | MS.gene04373:CDS | 65.0% | |
| ACAGGGACCAACCGGCCGAG+AGG | - | chr3.4:56955518-56955537 | None:intergenic | 70.0% | |
| GGGACCAACCGGCCGAGAGG+CGG | - | chr3.4:56955515-56955534 | None:intergenic | 75.0% | |
| TTCGCCGCCTCTCGGCCGGT+TGG | + | chr3.4:56955508-56955527 | MS.gene04373:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 56955155 | 56955641 | 56955155 | ID=MS.gene04373 |
| chr3.4 | mRNA | 56955155 | 56955641 | 56955155 | ID=MS.gene04373.t1;Parent=MS.gene04373 |
| chr3.4 | exon | 56955155 | 56955292 | 56955155 | ID=MS.gene04373.t1.exon1;Parent=MS.gene04373.t1 |
| chr3.4 | CDS | 56955155 | 56955292 | 56955155 | ID=cds.MS.gene04373.t1;Parent=MS.gene04373.t1 |
| chr3.4 | exon | 56955396 | 56955641 | 56955396 | ID=MS.gene04373.t1.exon2;Parent=MS.gene04373.t1 |
| chr3.4 | CDS | 56955396 | 56955641 | 56955396 | ID=cds.MS.gene04373.t1;Parent=MS.gene04373.t1 |
| Gene Sequence |
| Protein sequence |
