Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043735.t1 | XP_003622663.1 | 83.4 | 205 | 31 | 3 | 14 | 217 | 113 | 315 | 9.10E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043735.t1 | Q8S2E6 | 31.6 | 155 | 90 | 5 | 45 | 192 | 234 | 379 | 6.6e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043735.t1 | G7KVV4 | 83.4 | 205 | 31 | 3 | 14 | 217 | 113 | 315 | 6.6e-88 | 333.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene043735.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043735.t1 | MTR_7g050560 | 83.415 | 205 | 31 | 3 | 14 | 217 | 113 | 315 | 3.52e-120 | 344 |
| MS.gene043735.t1 | MTR_7g050590 | 69.856 | 209 | 45 | 4 | 14 | 205 | 86 | 293 | 1.33e-96 | 283 |
| MS.gene043735.t1 | MTR_7g050580 | 68.718 | 195 | 45 | 3 | 23 | 217 | 137 | 315 | 4.62e-83 | 249 |
| MS.gene043735.t1 | MTR_3g098790 | 66.120 | 183 | 55 | 2 | 23 | 205 | 137 | 312 | 8.07e-79 | 239 |
| MS.gene043735.t1 | MTR_7g050710 | 64.865 | 185 | 62 | 2 | 23 | 205 | 137 | 320 | 4.21e-78 | 237 |
| MS.gene043735.t1 | MTR_7g050720 | 53.846 | 182 | 75 | 5 | 22 | 194 | 136 | 317 | 4.85e-56 | 181 |
| MS.gene043735.t1 | MTR_1g035460 | 41.441 | 111 | 54 | 3 | 82 | 192 | 188 | 287 | 6.40e-20 | 87.0 |
| MS.gene043735.t1 | MTR_1g108780 | 30.168 | 179 | 100 | 6 | 15 | 192 | 136 | 290 | 4.06e-15 | 73.6 |
| MS.gene043735.t1 | MTR_8g103940 | 34.483 | 174 | 90 | 8 | 31 | 192 | 170 | 331 | 5.64e-15 | 73.6 |
| MS.gene043735.t1 | MTR_4g068320 | 31.579 | 133 | 85 | 2 | 65 | 197 | 267 | 393 | 1.11e-13 | 69.3 |
| MS.gene043735.t1 | MTR_1g034240 | 29.801 | 151 | 96 | 3 | 65 | 211 | 271 | 415 | 2.00e-13 | 68.9 |
| MS.gene043735.t1 | MTR_3g061320 | 34.247 | 146 | 73 | 7 | 47 | 191 | 156 | 279 | 7.97e-12 | 63.5 |
| MS.gene043735.t1 | MTR_3g061370 | 31.298 | 131 | 81 | 5 | 62 | 191 | 159 | 281 | 5.30e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043735.t1 | AT1G49480 | 32.000 | 125 | 78 | 3 | 68 | 192 | 104 | 221 | 6.47e-13 | 65.9 |
| MS.gene043735.t1 | AT1G49480 | 32.000 | 125 | 78 | 3 | 68 | 192 | 104 | 221 | 6.47e-13 | 65.9 |
| MS.gene043735.t1 | AT1G49480 | 32.000 | 125 | 78 | 3 | 68 | 192 | 104 | 221 | 6.47e-13 | 65.9 |
| MS.gene043735.t1 | AT3G18990 | 32.061 | 131 | 82 | 3 | 65 | 195 | 216 | 339 | 2.61e-12 | 65.5 |
Find 35 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATCAACCATGTGAAAT+TGG | 0.211929 | 7.4:-53075819 | MS.gene043735:CDS |
| GAGAGGAGATGTTTATTTGC+AGG | 0.289134 | 7.4:-53075006 | MS.gene043735:CDS |
| CTACACAGTATGACCCTTAC+AGG | 0.330799 | 7.4:-53074951 | MS.gene043735:CDS |
| AGGTGTTAAATGAAGGAGTT+TGG | 0.403488 | 7.4:-53074986 | MS.gene043735:CDS |
| GTCTAAAATGTGGTCCTGTA+AGG | 0.417430 | 7.4:+53074937 | None:intergenic |
| ATACTGTGTAGGTACTTTGC+AGG | 0.422142 | 7.4:+53074963 | None:intergenic |
| GTTGCAGCTAAAGAGGTATC+TGG | 0.442534 | 7.4:-53075873 | MS.gene043735:CDS |
| CCAAAGGGTTGCAGCTAAAG+AGG | 0.454060 | 7.4:-53075880 | MS.gene043735:intron |
| GAAGAAGCTTCTCAAGGATA+AGG | 0.472251 | 7.4:-53074661 | None:intergenic |
| TATTTGCAGGTGTTAAATGA+AGG | 0.472581 | 7.4:-53074993 | MS.gene043735:CDS |
| AACAATGAAGGTAAGTATAG+TGG | 0.478351 | 7.4:+53074836 | None:intergenic |
| CATATCTGAAAAGCAGAAGA+TGG | 0.486489 | 7.4:+53075967 | None:intergenic |
| GAGAAGCTTCTTCTTTCACT+TGG | 0.486869 | 7.4:+53074671 | None:intergenic |
| AAGGACAATAACTTAAAAGT+TGG | 0.486873 | 7.4:-53074888 | MS.gene043735:CDS |
| TGAAGTTGAGAAATTGCAAA+AGG | 0.487851 | 7.4:-53075781 | MS.gene043735:CDS |
| CTCTCTTCTTATCCAACTCA+AGG | 0.506333 | 7.4:+53075024 | None:intergenic |
| TCTAAAGATGTGAACAATGA+AGG | 0.509907 | 7.4:+53074824 | None:intergenic |
| TGGAACTTACCAGAAGAAAA+CGG | 0.543455 | 7.4:+53075320 | None:intergenic |
| CAGAATATACCACGTATGCT+TGG | 0.544975 | 7.4:-53075056 | MS.gene043735:CDS |
| CGCATGACAACAACAAAGGA+TGG | 0.548509 | 7.4:+53075361 | None:intergenic |
| CCATTGCTTCCAATTTCACA+TGG | 0.553545 | 7.4:+53075810 | None:intergenic |
| TCTAAAATGTGGTCCTGTAA+GGG | 0.557222 | 7.4:+53074938 | None:intergenic |
| TAGACTCTCAGGTGGATGGA+AGG | 0.562253 | 7.4:-53074919 | MS.gene043735:CDS |
| CCATGTGAAATTGGAAGCAA+TGG | 0.565270 | 7.4:-53075810 | MS.gene043735:CDS |
| AGAAGCTTCTTCTTTCACTT+GGG | 0.579559 | 7.4:+53074672 | None:intergenic |
| TGGTAATAGACACCTTGAGT+TGG | 0.592518 | 7.4:-53075036 | MS.gene043735:CDS |
| GTGAAAGAAGAAGCTTCTCA+AGG | 0.611713 | 7.4:-53074667 | MS.gene043735:CDS |
| CTGTAAGGGTCATACTGTGT+AGG | 0.612290 | 7.4:+53074952 | None:intergenic |
| TGGTTGATGAAATTCAAAAG+AGG | 0.621012 | 7.4:+53075830 | None:intergenic |
| CTTGAGTTGGATAAGAAGAG+AGG | 0.623819 | 7.4:-53075023 | MS.gene043735:CDS |
| TGGATGGAAGGCATTTGCAA+AGG | 0.640290 | 7.4:-53074907 | MS.gene043735:CDS |
| CCACCTGAGAGTCTAAAATG+TGG | 0.688979 | 7.4:+53074927 | None:intergenic |
| TGTCTATTACCAAGCATACG+TGG | 0.689818 | 7.4:+53075047 | None:intergenic |
| CATGCTGATAGAGAGTCCAA+AGG | 0.692736 | 7.4:-53075744 | MS.gene043735:intron |
| TGCACGCATGACAACAACAA+AGG | 0.693553 | 7.4:+53075357 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGAAATGAAATAAAAAAA+TGG | + | chr7.4:53075106-53075125 | None:intergenic | 10.0% |
| !! | TTTCTTAAAAAACCTTTAAA+TGG | - | chr7.4:53075119-53075138 | MS.gene043735:intron | 15.0% |
| !! | ATGAAATAAAAAAATGGAGA+AGG | + | chr7.4:53075100-53075119 | None:intergenic | 20.0% |
| ! | AAGGACAATAACTTAAAAGT+TGG | - | chr7.4:53075746-53075765 | MS.gene043735:CDS | 25.0% |
| ! | AGATAAATTATCCTTTCCAA+AGG | - | chr7.4:53074738-53074757 | MS.gene043735:intron | 25.0% |
| ! | GAAAGGATAATTTATCTCTA+AGG | + | chr7.4:53074735-53074754 | None:intergenic | 25.0% |
| ! | GATAAATTATCCTTTCCAAA+GGG | - | chr7.4:53074739-53074758 | MS.gene043735:intron | 25.0% |
| ! | TCTATGTGATGATATCATAA+TGG | + | chr7.4:53075405-53075424 | None:intergenic | 25.0% |
| !! | TATTGTGTTCAAATTTTGCA+GGG | - | chr7.4:53075181-53075200 | MS.gene043735:intron | 25.0% |
| !! | TCAACATTTGTCTTTTCTTT+TGG | - | chr7.4:53075551-53075570 | MS.gene043735:intron | 25.0% |
| !! | TGAAAAAACTTCTCTTTTTG+AGG | - | chr7.4:53074688-53074707 | MS.gene043735:intron | 25.0% |
| !! | TTATTGTGTTCAAATTTTGC+AGG | - | chr7.4:53075180-53075199 | MS.gene043735:intron | 25.0% |
| !!! | GCTTTTAGAAAATAAGTTGA+TGG | - | chr7.4:53075349-53075368 | MS.gene043735:CDS | 25.0% |
| AACAATGAAGGTAAGTATAG+TGG | + | chr7.4:53075801-53075820 | None:intergenic | 30.0% | |
| AAGACACAATCTTATACCTT+TGG | + | chr7.4:53074909-53074928 | None:intergenic | 30.0% | |
| ATGGTCTTTACTAGATTAAG+GGG | - | chr7.4:53075368-53075387 | MS.gene043735:CDS | 30.0% | |
| CTAAAAGCCAAATGTTTCTT+TGG | + | chr7.4:53075337-53075356 | None:intergenic | 30.0% | |
| GATGGTCTTTACTAGATTAA+GGG | - | chr7.4:53075367-53075386 | MS.gene043735:CDS | 30.0% | |
| GTAAGTTCCAAAGAAACATT+TGG | - | chr7.4:53075327-53075346 | MS.gene043735:intron | 30.0% | |
| TATTTGCAGGTGTTAAATGA+AGG | - | chr7.4:53075641-53075660 | MS.gene043735:intron | 30.0% | |
| TCTAAAGATGTGAACAATGA+AGG | + | chr7.4:53075813-53075832 | None:intergenic | 30.0% | |
| TGAAGTTGAGAAATTGCAAA+AGG | - | chr7.4:53074853-53074872 | MS.gene043735:CDS | 30.0% | |
| TGATGGTCTTTACTAGATTA+AGG | - | chr7.4:53075366-53075385 | MS.gene043735:CDS | 30.0% | |
| TGTTCTACAATAACTCTGAA+GGG | + | chr7.4:53075041-53075060 | None:intergenic | 30.0% | |
| TTTCATCAACCATGTGAAAT+TGG | - | chr7.4:53074815-53074834 | MS.gene043735:CDS | 30.0% | |
| ! | CATTTGTCTTTTCTTTTGGT+TGG | - | chr7.4:53075555-53075574 | MS.gene043735:intron | 30.0% |
| !! | TGGTTGATGAAATTCAAAAG+AGG | + | chr7.4:53074807-53074826 | None:intergenic | 30.0% |
| AGAAGCTTCTTCTTTCACTT+GGG | + | chr7.4:53075965-53075984 | None:intergenic | 35.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | - | chr7.4:53075648-53075667 | MS.gene043735:intron | 35.0% | |
| ATGAGCTGCAAACCATTTAA+AGG | + | chr7.4:53075134-53075153 | None:intergenic | 35.0% | |
| CATATCTGAAAAGCAGAAGA+TGG | + | chr7.4:53074670-53074689 | None:intergenic | 35.0% | |
| CTGTTCTACAATAACTCTGA+AGG | + | chr7.4:53075042-53075061 | None:intergenic | 35.0% | |
| TGGAACTTACCAGAAGAAAA+CGG | + | chr7.4:53075317-53075336 | None:intergenic | 35.0% | |
| TTCGTTCAATGATGTATCGA+TGG | - | chr7.4:53075002-53075021 | MS.gene043735:CDS | 35.0% | |
| ! | TCTAAAATGTGGTCCTGTAA+GGG | + | chr7.4:53075699-53075718 | None:intergenic | 35.0% |
| ATACTGTGTAGGTACTTTGC+AGG | + | chr7.4:53075674-53075693 | None:intergenic | 40.0% | |
| CAGAATATACCACGTATGCT+TGG | - | chr7.4:53075578-53075597 | MS.gene043735:intron | 40.0% | |
| CCATGTGAAATTGGAAGCAA+TGG | - | chr7.4:53074824-53074843 | MS.gene043735:CDS | 40.0% | |
| CCATTGCTTCCAATTTCACA+TGG | + | chr7.4:53074827-53074846 | None:intergenic | 40.0% | |
| CTCTCTTCTTATCCAACTCA+AGG | + | chr7.4:53075613-53075632 | None:intergenic | 40.0% | |
| GAGAAGCTTCTTCTTTCACT+TGG | + | chr7.4:53075966-53075985 | None:intergenic | 40.0% | |
| GAGAGGAGATGTTTATTTGC+AGG | - | chr7.4:53075628-53075647 | MS.gene043735:intron | 40.0% | |
| GTGAAAGAAGAAGCTTCTCA+AGG | - | chr7.4:53075967-53075986 | MS.gene043735:CDS | 40.0% | |
| TGGTAATAGACACCTTGAGT+TGG | - | chr7.4:53075598-53075617 | MS.gene043735:intron | 40.0% | |
| TGTCTATTACCAAGCATACG+TGG | + | chr7.4:53075590-53075609 | None:intergenic | 40.0% | |
| ! | ATTTTAGACTCTCAGGTGGA+TGG | - | chr7.4:53075711-53075730 | MS.gene043735:intron | 40.0% |
| ! | CGTTAAACACCGTTTTCTTC+TGG | - | chr7.4:53075305-53075324 | MS.gene043735:intron | 40.0% |
| ! | GTCTAAAATGTGGTCCTGTA+AGG | + | chr7.4:53075700-53075719 | None:intergenic | 40.0% |
| !! | CTTGAGTTGGATAAGAAGAG+AGG | - | chr7.4:53075611-53075630 | MS.gene043735:intron | 40.0% |
| CATGCTGATAGAGAGTCCAA+AGG | - | chr7.4:53074890-53074909 | MS.gene043735:CDS | 45.0% | |
| CGCATGACAACAACAAAGGA+TGG | + | chr7.4:53075276-53075295 | None:intergenic | 45.0% | |
| CTACACAGTATGACCCTTAC+AGG | - | chr7.4:53075683-53075702 | MS.gene043735:intron | 45.0% | |
| CTGTAAGGGTCATACTGTGT+AGG | + | chr7.4:53075685-53075704 | None:intergenic | 45.0% | |
| TGCACGCATGACAACAACAA+AGG | + | chr7.4:53075280-53075299 | None:intergenic | 45.0% | |
| ! | CCACATTTTAGACTCTCAGG+TGG | - | chr7.4:53075707-53075726 | MS.gene043735:intron | 45.0% |
| ! | CCACCTGAGAGTCTAAAATG+TGG | + | chr7.4:53075710-53075729 | None:intergenic | 45.0% |
| ! | GGACCACATTTTAGACTCTC+AGG | - | chr7.4:53075704-53075723 | MS.gene043735:intron | 45.0% |
| ! | TTAGCTGCAACCCTTTGGAA+AGG | + | chr7.4:53074752-53074771 | None:intergenic | 45.0% |
| !! | GTTGCAGCTAAAGAGGTATC+TGG | - | chr7.4:53074761-53074780 | MS.gene043735:intron | 45.0% |
| !! | TGGATGGAAGGCATTTGCAA+AGG | - | chr7.4:53075727-53075746 | MS.gene043735:intron | 45.0% |
| !! | TATATATTATAATAACAAAA+TGG | - | chr7.4:53075447-53075466 | MS.gene043735:intron | 5.0% |
| CCAAAGGGTTGCAGCTAAAG+AGG | - | chr7.4:53074754-53074773 | MS.gene043735:intron | 50.0% | |
| CCTCTTTAGCTGCAACCCTT+TGG | + | chr7.4:53074757-53074776 | None:intergenic | 50.0% | |
| TAGACTCTCAGGTGGATGGA+AGG | - | chr7.4:53075715-53075734 | MS.gene043735:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 53074663 | 53075993 | 53074663 | ID=MS.gene043735 |
| chr7.4 | mRNA | 53074663 | 53075993 | 53074663 | ID=MS.gene043735.t1;Parent=MS.gene043735 |
| chr7.4 | exon | 53075947 | 53075993 | 53075947 | ID=MS.gene043735.t1.exon1;Parent=MS.gene043735.t1 |
| chr7.4 | CDS | 53075947 | 53075993 | 53075947 | ID=cds.MS.gene043735.t1;Parent=MS.gene043735.t1 |
| chr7.4 | exon | 53075745 | 53075896 | 53075745 | ID=MS.gene043735.t1.exon2;Parent=MS.gene043735.t1 |
| chr7.4 | CDS | 53075745 | 53075896 | 53075745 | ID=cds.MS.gene043735.t1;Parent=MS.gene043735.t1 |
| chr7.4 | exon | 53075330 | 53075454 | 53075330 | ID=MS.gene043735.t1.exon3;Parent=MS.gene043735.t1 |
| chr7.4 | CDS | 53075330 | 53075454 | 53075330 | ID=cds.MS.gene043735.t1;Parent=MS.gene043735.t1 |
| chr7.4 | exon | 53074784 | 53075075 | 53074784 | ID=MS.gene043735.t1.exon4;Parent=MS.gene043735.t1 |
| chr7.4 | CDS | 53074784 | 53075075 | 53074784 | ID=cds.MS.gene043735.t1;Parent=MS.gene043735.t1 |
| chr7.4 | exon | 53074663 | 53074700 | 53074663 | ID=MS.gene043735.t1.exon5;Parent=MS.gene043735.t1 |
| chr7.4 | CDS | 53074663 | 53074700 | 53074663 | ID=cds.MS.gene043735.t1;Parent=MS.gene043735.t1 |
| Gene Sequence |
| Protein sequence |
