Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043750.t1 | AES78884.2 | 78.8 | 212 | 37 | 2 | 1 | 204 | 82 | 293 | 3.20E-85 | 324.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043750.t1 | Q0JP99 | 35.6 | 135 | 73 | 4 | 77 | 207 | 133 | 257 | 2.1e-08 | 60.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043750.t1 | G7KVV7 | 78.8 | 212 | 37 | 2 | 1 | 204 | 82 | 293 | 2.3e-85 | 324.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene043750.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene043750.t1 | MTR_7g050590 | 78.774 | 212 | 37 | 2 | 1 | 204 | 82 | 293 | 6.18e-116 | 332 |
| MS.gene043750.t1 | MTR_7g050560 | 68.372 | 215 | 57 | 4 | 1 | 214 | 109 | 313 | 2.20e-98 | 288 |
| MS.gene043750.t1 | MTR_7g050580 | 65.596 | 218 | 56 | 3 | 2 | 214 | 110 | 313 | 2.56e-91 | 270 |
| MS.gene043750.t1 | MTR_7g050710 | 61.972 | 213 | 71 | 5 | 1 | 204 | 109 | 320 | 1.09e-82 | 249 |
| MS.gene043750.t1 | MTR_3g098790 | 61.905 | 210 | 68 | 4 | 1 | 204 | 109 | 312 | 2.89e-82 | 248 |
| MS.gene043750.t1 | MTR_7g050720 | 51.887 | 212 | 83 | 8 | 1 | 193 | 106 | 317 | 5.28e-60 | 191 |
| MS.gene043750.t1 | MTR_1g035460 | 39.844 | 128 | 64 | 5 | 81 | 208 | 188 | 302 | 2.00e-19 | 85.9 |
| MS.gene043750.t1 | MTR_1g108780 | 37.190 | 121 | 63 | 4 | 72 | 191 | 182 | 290 | 9.03e-18 | 81.3 |
| MS.gene043750.t1 | MTR_8g103940 | 33.152 | 184 | 94 | 7 | 23 | 191 | 162 | 331 | 8.46e-16 | 75.9 |
| MS.gene043750.t1 | MTR_1g035490 | 35.484 | 124 | 73 | 4 | 73 | 194 | 81 | 199 | 1.94e-12 | 65.5 |
| MS.gene043750.t1 | MTR_4g068320 | 31.148 | 122 | 78 | 2 | 74 | 195 | 277 | 392 | 1.56e-11 | 63.2 |
| MS.gene043750.t1 | MTR_5g044670 | 35.156 | 128 | 71 | 4 | 81 | 208 | 199 | 314 | 2.32e-11 | 62.4 |
| MS.gene043750.t1 | MTR_1g034240 | 29.134 | 127 | 84 | 2 | 65 | 191 | 272 | 392 | 5.84e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATCAACCACGTGAAAT+TGG | 0.222170 | 7.4:-53350290 | MS.gene043750:CDS |
| GGACTAAAATTTCAACTTAC+AGG | 0.245796 | 7.4:-53349280 | MS.gene043750:CDS |
| AGGTGTTAAATGAAGGAGTT+TGG | 0.351103 | 7.4:-53349336 | MS.gene043750:CDS |
| AAGTAGCTTCTTCTTTCATT+TGG | 0.368122 | 7.4:+53349019 | None:intergenic |
| TGGTAATAGACACTTTGATT+TGG | 0.391800 | 7.4:-53349386 | MS.gene043750:CDS |
| CAGAATATATCACGTTCGTT+TGG | 0.427346 | 7.4:-53349406 | MS.gene043750:CDS |
| ATGAAAGAAGAAGCTACTTA+AGG | 0.444131 | 7.4:-53349015 | None:intergenic |
| ACTTTGAGATGAAGAACAAT+TGG | 0.461943 | 7.4:+53349132 | None:intergenic |
| CAAGGAAATAAACGTTCAAA+TGG | 0.463400 | 7.4:-53350359 | MS.gene043750:CDS |
| CATTGGAAACTCGTTGGAAT+GGG | 0.484032 | 7.4:+53350384 | None:intergenic |
| TCTATCAAGAAGTGCAGTAA+AGG | 0.491677 | 7.4:-53349301 | MS.gene043750:CDS |
| TTTGATTTGGATAAGAAAAG+AGG | 0.493087 | 7.4:-53349373 | MS.gene043750:CDS |
| ACGTTTATTTCCTTGACCAT+TGG | 0.495512 | 7.4:+53350367 | None:intergenic |
| CTTGACCATTGGAAACTCGT+TGG | 0.499234 | 7.4:+53350378 | None:intergenic |
| CCATTGGAAACTCGTTGGAA+TGG | 0.503568 | 7.4:+53350383 | None:intergenic |
| GAGGAAAATAACTTGAAAGT+TGG | 0.503659 | 7.4:-53349238 | MS.gene043750:CDS |
| ATGAAGGAGTTTGGACTGCA+AGG | 0.527571 | 7.4:-53349327 | MS.gene043750:CDS |
| AATTTCAACTTACAGGTGGA+TGG | 0.530556 | 7.4:-53349273 | MS.gene043750:CDS |
| CGCATGACAACAACAAAGGA+TGG | 0.548509 | 7.4:+53350022 | None:intergenic |
| CAACGAGTTTCCAATGGTCA+AGG | 0.552700 | 7.4:-53350377 | MS.gene043750:CDS |
| ACGTGGTTGATGAAGTTCAA+AGG | 0.562344 | 7.4:+53350298 | None:intergenic |
| CCATTCCAACGAGTTTCCAA+TGG | 0.563382 | 7.4:-53350383 | MS.gene043750:CDS |
| TCAACTTACAGGTGGATGGA+AGG | 0.567817 | 7.4:-53349269 | MS.gene043750:CDS |
| CTAAAATTTCAACTTACAGG+TGG | 0.580366 | 7.4:-53349277 | MS.gene043750:CDS |
| TAGAGTTGGAAAACTGCAGA+AGG | 0.585587 | 7.4:-53350252 | MS.gene043750:CDS |
| CTACTGCATCCAATTTCACG+TGG | 0.656993 | 7.4:+53350281 | None:intergenic |
| TGGATGGAAGGCATTTGCAG+AGG | 0.673309 | 7.4:-53349257 | MS.gene043750:CDS |
| TGCACGCATGACAACAACAA+AGG | 0.693553 | 7.4:+53350018 | None:intergenic |
| CATACTGACAGAGAGTCCAA+AGG | 0.719746 | 7.4:-53350215 | MS.gene043750:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATATTCTAATTATCCT+TGG | + | chr7.4:53349572-53349591 | None:intergenic | 15.0% |
| !!! | AGTTATTTGATTATTTTGTT+AGG | - | chr7.4:53349615-53349634 | MS.gene043750:intron | 15.0% |
| !! | AAATGTGTATTAATCAAGAT+CGG | + | chr7.4:53349755-53349774 | None:intergenic | 20.0% |
| !! | TTAAGTTTCAATGACATATT+TGG | - | chr7.4:53349445-53349464 | MS.gene043750:intron | 20.0% |
| !!! | TGTTGTTATTTTGTTTATTC+TGG | - | chr7.4:53349652-53349671 | MS.gene043750:intron | 20.0% |
| !! | CATTTGTCTTTTCTTTTACT+TGG | - | chr7.4:53349980-53349999 | MS.gene043750:intron | 25.0% |
| !! | TATTTTGTTAGGTGATTGAT+AGG | - | chr7.4:53349626-53349645 | MS.gene043750:intron | 25.0% |
| !!! | TTTGATTTGGATAAGAAAAG+AGG | - | chr7.4:53350036-53350055 | MS.gene043750:CDS | 25.0% |
| AAGTAGCTTCTTCTTTCATT+TGG | + | chr7.4:53350393-53350412 | None:intergenic | 30.0% | |
| AAGTATTTGAATAACGCTTG+AGG | - | chr7.4:53349677-53349696 | MS.gene043750:intron | 30.0% | |
| AGTATTTGAATAACGCTTGA+GGG | - | chr7.4:53349678-53349697 | MS.gene043750:intron | 30.0% | |
| ATTGTTCTTCATCTCAAAGT+AGG | - | chr7.4:53350280-53350299 | MS.gene043750:CDS | 30.0% | |
| CAAGGAAATAAACGTTCAAA+TGG | - | chr7.4:53349050-53349069 | MS.gene043750:intron | 30.0% | |
| CTAAAATTTCAACTTACAGG+TGG | - | chr7.4:53350132-53350151 | MS.gene043750:intron | 30.0% | |
| GAGGAAAATAACTTGAAAGT+TGG | - | chr7.4:53350171-53350190 | MS.gene043750:intron | 30.0% | |
| GGACTAAAATTTCAACTTAC+AGG | - | chr7.4:53350129-53350148 | MS.gene043750:intron | 30.0% | |
| TATCTAATCATGATGTCACT+TGG | + | chr7.4:53349863-53349882 | None:intergenic | 30.0% | |
| TGCAAAATTGGAACACAATA+AGG | + | chr7.4:53349298-53349317 | None:intergenic | 30.0% | |
| TGGTAATAGACACTTTGATT+TGG | - | chr7.4:53350023-53350042 | MS.gene043750:CDS | 30.0% | |
| ! | AAGAGGAGATATTCATTTTC+AGG | - | chr7.4:53350053-53350072 | MS.gene043750:CDS | 30.0% |
| ! | TATTGTGTTCCAATTTTGCA+GGG | - | chr7.4:53349298-53349317 | MS.gene043750:CDS | 30.0% |
| ! | TTATTGTGTTCCAATTTTGC+AGG | - | chr7.4:53349297-53349316 | MS.gene043750:CDS | 30.0% |
| !! | ACTTTGAGATGAAGAACAAT+TGG | + | chr7.4:53350280-53350299 | None:intergenic | 30.0% |
| !!! | CATTTTCAGGTGTTAAATGA+AGG | - | chr7.4:53350066-53350085 | MS.gene043750:CDS | 30.0% |
| AAGCCACAATCTTATACCTT+TGG | + | chr7.4:53349213-53349232 | None:intergenic | 35.0% | |
| AATCTAGTAGAGAGTAGAGA+AGG | + | chr7.4:53349493-53349512 | None:intergenic | 35.0% | |
| AATTTCAACTTACAGGTGGA+TGG | - | chr7.4:53350136-53350155 | MS.gene043750:intron | 35.0% | |
| ACGTTTATTTCCTTGACCAT+TGG | + | chr7.4:53349045-53349064 | None:intergenic | 35.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | - | chr7.4:53350073-53350092 | MS.gene043750:CDS | 35.0% | |
| ATCTTTCCATTGATACCAAC+TGG | - | chr7.4:53349795-53349814 | MS.gene043750:intron | 35.0% | |
| CAAAAACTATGTATGCACCA+AGG | - | chr7.4:53349552-53349571 | MS.gene043750:intron | 35.0% | |
| CAGAATATATCACGTTCGTT+TGG | - | chr7.4:53350003-53350022 | MS.gene043750:CDS | 35.0% | |
| CATTTGGAAAGATTCTGCAT+AGG | + | chr7.4:53350377-53350396 | None:intergenic | 35.0% | |
| CTCTACTCTCTACTAGATTA+CGG | - | chr7.4:53349494-53349513 | MS.gene043750:intron | 35.0% | |
| GTATTTGAATAACGCTTGAG+GGG | - | chr7.4:53349679-53349698 | MS.gene043750:intron | 35.0% | |
| ! | AGTCCAAAGGTATAAGATTG+TGG | - | chr7.4:53349207-53349226 | MS.gene043750:CDS | 35.0% |
| ! | CGTTGAACATCATTTTCTAC+TGG | - | chr7.4:53349419-53349438 | MS.gene043750:intron | 35.0% |
| ! | TAATCATGATGTCACTTGGT+AGG | + | chr7.4:53349859-53349878 | None:intergenic | 35.0% |
| ! | TCTATCAAGAAGTGCAGTAA+AGG | - | chr7.4:53350108-53350127 | MS.gene043750:intron | 35.0% |
| !!! | TGCAGTAGTTGTTTTAGAGT+TGG | - | chr7.4:53349143-53349162 | MS.gene043750:CDS | 35.0% |
| CACTTCTTTCCCTGCAAAAT+TGG | + | chr7.4:53349310-53349329 | None:intergenic | 40.0% | |
| CATTGGAAACTCGTTGGAAT+GGG | + | chr7.4:53349028-53349047 | None:intergenic | 40.0% | |
| CTTCATCAACCACGTGAAAT+TGG | - | chr7.4:53349119-53349138 | MS.gene043750:intron | 40.0% | |
| GTAGAGAGTAGAGAAGGAAT+TGG | + | chr7.4:53349487-53349506 | None:intergenic | 40.0% | |
| TAGAGTTGGAAAACTGCAGA+AGG | - | chr7.4:53349157-53349176 | MS.gene043750:CDS | 40.0% | |
| TAGGATTGTCCATTTGAGCT+GGG | + | chr7.4:53349840-53349859 | None:intergenic | 40.0% | |
| TGGAAAGATTCTGCATAGGA+TGG | + | chr7.4:53350373-53350392 | None:intergenic | 40.0% | |
| !! | ACGTGGTTGATGAAGTTCAA+AGG | + | chr7.4:53349114-53349133 | None:intergenic | 40.0% |
| ATGAAGGAGTTTGGACTGCA+AGG | - | chr7.4:53350082-53350101 | MS.gene043750:CDS | 45.0% | |
| ATTTCACAACAGGGACCAGT+TGG | + | chr7.4:53349813-53349832 | None:intergenic | 45.0% | |
| CAACGAGTTTCCAATGGTCA+AGG | - | chr7.4:53349032-53349051 | MS.gene043750:intron | 45.0% | |
| CATACTGACAGAGAGTCCAA+AGG | - | chr7.4:53349194-53349213 | MS.gene043750:CDS | 45.0% | |
| CCATTCCAACGAGTTTCCAA+TGG | - | chr7.4:53349026-53349045 | MS.gene043750:CDS | 45.0% | |
| CCATTGGAAACTCGTTGGAA+TGG | + | chr7.4:53349029-53349048 | None:intergenic | 45.0% | |
| CGCATGACAACAACAAAGGA+TGG | + | chr7.4:53349390-53349409 | None:intergenic | 45.0% | |
| CTACTGCATCCAATTTCACG+TGG | + | chr7.4:53349131-53349150 | None:intergenic | 45.0% | |
| CTGGGCACAATTTCACAACA+GGG | + | chr7.4:53349822-53349841 | None:intergenic | 45.0% | |
| CTTGACCATTGGAAACTCGT+TGG | + | chr7.4:53349034-53349053 | None:intergenic | 45.0% | |
| GAAATTGTGCCCAGCTCAAA+TGG | - | chr7.4:53349828-53349847 | MS.gene043750:intron | 45.0% | |
| GTAGGATTGTCCATTTGAGC+TGG | + | chr7.4:53349841-53349860 | None:intergenic | 45.0% | |
| TCAACTTACAGGTGGATGGA+AGG | - | chr7.4:53350140-53350159 | MS.gene043750:intron | 45.0% | |
| TGAATAACGCTTGAGGGGAT+GGG | - | chr7.4:53349684-53349703 | MS.gene043750:intron | 45.0% | |
| TGCACGCATGACAACAACAA+AGG | + | chr7.4:53349394-53349413 | None:intergenic | 45.0% | |
| TTGAATAACGCTTGAGGGGA+TGG | - | chr7.4:53349683-53349702 | MS.gene043750:intron | 45.0% | |
| GCTGGGCACAATTTCACAAC+AGG | + | chr7.4:53349823-53349842 | None:intergenic | 50.0% | |
| ! | CAGGGACCAGTTGGTATCAA+TGG | + | chr7.4:53349804-53349823 | None:intergenic | 50.0% |
| !! | TGGATGGAAGGCATTTGCAG+AGG | - | chr7.4:53350152-53350171 | MS.gene043750:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 53349017 | 53350414 | 53349017 | ID=MS.gene043750 |
| chr7.4 | mRNA | 53349017 | 53350414 | 53349017 | ID=MS.gene043750.t1;Parent=MS.gene043750 |
| chr7.4 | exon | 53350216 | 53350414 | 53350216 | ID=MS.gene043750.t1.exon1;Parent=MS.gene043750.t1 |
| chr7.4 | CDS | 53350216 | 53350414 | 53350216 | ID=cds.MS.gene043750.t1;Parent=MS.gene043750.t1 |
| chr7.4 | exon | 53349991 | 53350112 | 53349991 | ID=MS.gene043750.t1.exon2;Parent=MS.gene043750.t1 |
| chr7.4 | CDS | 53349991 | 53350112 | 53349991 | ID=cds.MS.gene043750.t1;Parent=MS.gene043750.t1 |
| chr7.4 | exon | 53349134 | 53349425 | 53349134 | ID=MS.gene043750.t1.exon3;Parent=MS.gene043750.t1 |
| chr7.4 | CDS | 53349134 | 53349425 | 53349134 | ID=cds.MS.gene043750.t1;Parent=MS.gene043750.t1 |
| chr7.4 | exon | 53349017 | 53349048 | 53349017 | ID=MS.gene043750.t1.exon4;Parent=MS.gene043750.t1 |
| chr7.4 | CDS | 53349017 | 53349048 | 53349017 | ID=cds.MS.gene043750.t1;Parent=MS.gene043750.t1 |
| Gene Sequence |
| Protein sequence |
