AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043872

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043872.t1 MTR_6g051425 30.182 275 175 6 50 311 19 289 2.36e-35 137
MS.gene043872.t1 MTR_0856s0010 43.023 172 84 4 3 173 2 160 3.25e-33 125
MS.gene043872.t1 MTR_4g035820 29.831 295 183 11 9 288 39 324 3.92e-24 108
MS.gene043872.t1 MTR_0558s0020 25.260 289 182 8 30 284 8 296 1.02e-21 96.7
MS.gene043872.t1 MTR_1g070355 28.936 235 152 7 54 278 80 309 2.06e-20 96.3
MS.gene043872.t1 MTR_7g446060 51.220 82 40 0 130 211 7 88 6.01e-20 87.0
MS.gene043872.t1 MTR_7g446480 32.558 172 115 1 122 292 143 314 1.40e-17 85.1
MS.gene043872.t1 MTR_8g446020 24.576 236 157 4 52 284 39 256 1.87e-17 83.2
MS.gene043872.t1 MTR_3g450850 26.481 287 192 8 2 272 3 286 3.95e-17 84.3
MS.gene043872.t1 MTR_7g446510 33.133 166 110 1 128 292 164 329 4.71e-17 83.6
MS.gene043872.t1 MTR_6g022070 28.966 145 99 1 338 482 1 141 4.85e-17 83.6
MS.gene043872.t1 MTR_7g446520 26.271 236 161 2 52 286 92 315 6.76e-17 83.2
MS.gene043872.t1 MTR_1g008290 32.331 133 88 1 108 240 14 144 1.00e-16 78.2
MS.gene043872.t1 MTR_7g115320 61.111 54 18 1 137 190 9 59 9.01e-15 70.9
MS.gene043872.t1 MTR_1g083640 23.162 272 175 8 18 255 11 282 3.15e-14 74.3
MS.gene043872.t1 MTR_1g019850 30.303 165 98 6 17 175 43 196 3.24e-14 73.6
MS.gene043872.t1 MTR_5g076070 23.552 259 185 7 23 272 28 282 1.95e-13 72.0
MS.gene043872.t1 MTR_5g023310 28.662 157 43 3 179 334 16 104 3.05e-13 67.0
MS.gene043872.t1 MTR_3g012160 40.741 81 47 1 193 273 14 93 5.50e-13 65.9
MS.gene043872.t1 MTR_6g090505 25.843 178 129 2 109 283 53 230 2.78e-12 69.7
MS.gene043872.t1 MTR_0402s0040 25.000 180 130 3 47 221 25 204 4.58e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043872.t1 AT4G15090 32.153 734 457 15 48 771 51 753 5.98e-123 389
MS.gene043872.t1 AT4G15090 32.153 734 457 15 48 771 51 753 5.98e-123 389
MS.gene043872.t1 AT4G15090 32.153 734 457 15 48 771 51 753 5.98e-123 389
MS.gene043872.t1 AT4G15090 32.153 734 457 15 48 771 51 753 5.98e-123 389
MS.gene043872.t1 AT4G15090 32.153 734 457 15 48 771 51 753 5.98e-123 389
MS.gene043872.t1 AT4G38180 31.978 738 437 18 8 705 38 750 3.15e-119 378
MS.gene043872.t1 AT3G22170 29.782 779 471 14 18 770 35 763 1.57e-111 359
MS.gene043872.t1 AT3G22170 29.782 779 471 14 18 770 35 763 1.57e-111 359
MS.gene043872.t1 AT1G76320 32.027 587 375 9 52 633 1 568 4.48e-105 337
MS.gene043872.t1 AT1G76320 32.027 587 375 9 52 633 1 568 1.03e-104 338
MS.gene043872.t1 AT1G76320 32.027 587 375 9 52 633 1 568 1.03e-104 338
MS.gene043872.t1 AT1G76320 32.027 587 375 9 52 633 1 568 1.14e-104 338
MS.gene043872.t1 AT1G52520 32.709 587 370 12 33 607 66 639 2.21e-104 336
MS.gene043872.t1 AT2G27110 31.803 610 363 14 48 645 49 617 7.22e-94 312
MS.gene043872.t1 AT2G27110 31.803 610 363 14 48 645 49 617 7.22e-94 312
MS.gene043872.t1 AT3G06250 33.215 563 324 14 48 606 190 704 2.21e-92 306
MS.gene043872.t1 AT3G06250 33.215 563 324 14 48 606 190 704 2.21e-92 306
MS.gene043872.t1 AT3G06250 33.215 563 324 14 48 606 190 704 2.21e-92 306
MS.gene043872.t1 AT1G80010 32.226 602 360 13 39 610 82 665 2.25e-90 300
MS.gene043872.t1 AT2G27110 34.937 478 288 9 172 645 14 472 5.81e-89 295
MS.gene043872.t1 AT5G18960 31.736 605 352 15 28 622 192 745 4.81e-86 290
MS.gene043872.t1 AT5G18960 31.736 605 352 15 28 622 192 745 4.81e-86 290
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 2.24e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 135 564 2.50e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 135 564 2.91e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 4.56e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 4.56e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 4.59e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 4.59e-82 279
MS.gene043872.t1 AT2G32250 34.537 443 269 6 224 658 176 605 4.59e-82 279
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 24 583 3.04e-80 273
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 24 583 3.77e-80 273
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 24 583 3.77e-80 273
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 38 597 4.18e-80 273
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 24 583 4.91e-80 273
MS.gene043872.t1 AT4G19990 30.363 606 352 12 51 632 24 583 4.91e-80 273
MS.gene043872.t1 AT4G19990 31.169 539 317 10 104 632 32 526 4.21e-76 260
MS.gene043872.t1 AT4G38170 33.763 388 246 4 221 606 21 399 4.86e-76 256
MS.gene043872.t1 AT1G10240 26.884 584 384 15 42 603 42 604 7.15e-63 224
MS.gene043872.t1 AT1G10240 26.884 584 384 15 42 603 42 604 7.15e-63 224
MS.gene043872.t1 AT5G28530 27.441 594 367 12 46 603 53 618 3.32e-61 219
MS.gene043872.t1 AT5G28530 26.936 594 366 11 46 603 53 614 2.41e-60 217
MS.gene043872.t1 AT5G28530 26.936 594 366 11 46 603 53 614 2.41e-60 217
MS.gene043872.t1 AT5G28530 26.220 328 204 6 290 603 252 555 1.39e-27 119
MS.gene043872.t1 AT5G28530 28.324 173 107 5 46 205 53 221 7.68e-11 66.2
MS.gene043872.t1 AT5G28530 25.305 328 203 5 290 603 252 551 7.57e-27 117
MS.gene043872.t1 AT5G28530 28.324 173 107 5 46 205 53 221 7.84e-11 66.2

Find 158 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGTTGACGTTTCGGATTC+CGG 0.204382 2.4:+31300493 MS.gene043872:CDS
TCTTTCCCGCTCTTCGAAAA+TGG 0.249066 2.4:-31301655 None:intergenic
TAAGATGACCAAGTACAAAA+AGG 0.265908 2.4:-31302157 None:intergenic
GACAATACTCTCCTCTAAAA+TGG 0.290898 2.4:-31302024 None:intergenic
GATCGCAAACCCTTGTTTCA+TGG 0.299904 2.4:-31300620 None:intergenic
AAGACCGCAACCTAGTAAAA+TGG 0.321194 2.4:-31301301 None:intergenic
GTGTTGACGTTTCGGATTCC+GGG 0.344560 2.4:+31300494 MS.gene043872:CDS
GGCTATGCAGAATGCAATTC+AGG 0.347065 2.4:+31301425 MS.gene043872:CDS
TTGCTTTAAAGAAGGGTCTA+TGG 0.361166 2.4:+31300791 MS.gene043872:CDS
TGCGGCTCGTGTTGACGTTT+CGG 0.362249 2.4:+31300486 MS.gene043872:CDS
ATGCCATTTGCCCCGTTTGT+TGG 0.369173 2.4:+31301258 MS.gene043872:CDS
ACTAAATCACGGTTGTTTGC+TGG 0.375082 2.4:+31300859 MS.gene043872:CDS
AGTATTGTGTTGATTTCAAC+GGG 0.389824 2.4:+31302069 MS.gene043872:CDS
CACCACCTATGCCTAGTATT+AGG 0.393202 2.4:-31301456 None:intergenic
GTACACCCATGACAAGTATA+TGG 0.398379 2.4:+31301923 MS.gene043872:CDS
GGATTAAGAATTGGTTGATC+AGG 0.405554 2.4:-31302491 None:intergenic
CTAGGCATAGGTGGTGTCTA+TGG 0.405638 2.4:+31301463 MS.gene043872:CDS
ACTTCGACAACCGAAACAAT+TGG 0.413255 2.4:-31302611 None:intergenic
AAAGGATGATCGATCAATAT+AGG 0.415391 2.4:-31300433 None:intergenic
ACATTACTTCCTATGTCTGA+TGG 0.423977 2.4:+31302743 MS.gene043872:CDS
CCTCTGTGTTGTTGACATTC+CGG 0.424793 2.4:-31301712 None:intergenic
GATGATTGACACCAACAAAC+GGG 0.432575 2.4:-31301269 None:intergenic
TTGGTGGTATGTTTGATTCA+TGG 0.433399 2.4:-31300717 None:intergenic
ACACCAACAAACGGGGCAAA+TGG 0.438957 2.4:-31301261 None:intergenic
GCAAACAACCGTGATTTAGT+TGG 0.439208 2.4:-31300856 None:intergenic
ATGGTTTGGGTGAAAATGAT+TGG 0.445440 2.4:+31301625 MS.gene043872:CDS
CATTACTTCCTATGTCTGAT+GGG 0.447968 2.4:+31302744 MS.gene043872:CDS
TGATGATTGACACCAACAAA+CGG 0.449717 2.4:-31301270 None:intergenic
CCATCAGCTAGCTCTCCGTC+AGG 0.451558 2.4:-31300463 None:intergenic
ACACAATACTTAGTTTCGTT+CGG 0.457073 2.4:-31302056 None:intergenic
GGCTAAGGTGTATGTCTTCG+AGG 0.457503 2.4:+31301370 MS.gene043872:CDS
TATCCTACTTTGCTAGAATG+AGG 0.459924 2.4:+31301082 MS.gene043872:CDS
AAAACTAGAAACTCTAAGAA+AGG 0.460138 2.4:+31300643 MS.gene043872:CDS
AAACTAGAAACTCTAAGAAA+GGG 0.460938 2.4:+31300644 MS.gene043872:CDS
ACTCCAAGACAAGGCTGAAA+AGG 0.462412 2.4:+31301818 MS.gene043872:CDS
CAAATGGTCTACCAAGAAGT+TGG 0.463702 2.4:+31302388 MS.gene043872:CDS
AGGTTGTGTTTCCTAATACT+AGG 0.465284 2.4:+31301445 MS.gene043872:CDS
GCAAAAGAAAGACACCTTCT+CGG 0.472868 2.4:+31301033 MS.gene043872:CDS
GATAACGGTTGCTTTAAAGA+AGG 0.473052 2.4:+31300783 MS.gene043872:CDS
AACATCACCAAAGTATTCAT+AGG 0.473828 2.4:-31301196 None:intergenic
TTTGTTGGTGTCAATCATCA+TGG 0.475674 2.4:+31301273 MS.gene043872:CDS
CCACACATAGAAAGATTTGA+AGG 0.478166 2.4:+31302284 MS.gene043872:CDS
GCCACATTGTGGTGTGGGAG+GGG 0.479684 2.4:+31302427 MS.gene043872:CDS
GAGCAATCAAGTTTCTTCGT+TGG 0.480101 2.4:-31300754 None:intergenic
CACTTATGCTCGATGGCTGA+TGG 0.482201 2.4:+31300406 None:intergenic
TCGGGAAGGATGGAGATGGC+AGG 0.482875 2.4:+31301052 MS.gene043872:CDS
ATTTGTTGTTTCAAAGGTCC+CGG 0.485747 2.4:-31300512 None:intergenic
TTGAAATTGTCTCTCGATTG+TGG 0.489013 2.4:-31301895 None:intergenic
GACAGCATTTGTTGTTTCAA+AGG 0.491833 2.4:-31300518 None:intergenic
CTTGTTTCATGGCATATTCA+CGG 0.492232 2.4:-31300609 None:intergenic
AAAACCACTAGTTGACTGTT+GGG 0.492621 2.4:-31302804 None:intergenic
TCCGTTAAAAGTACCACGTA+AGG 0.492742 2.4:+31302511 MS.gene043872:CDS
TGTGCTTGAAGACCGAAAAT+CGG 0.495339 2.4:-31301850 None:intergenic
CGTGAATATGCCATGAAACA+AGG 0.498225 2.4:+31300610 MS.gene043872:CDS
TGTATGAAGAAATGATCAAA+TGG 0.501577 2.4:+31302372 MS.gene043872:CDS
TTAAAAGACATACCGATAGT+AGG 0.501742 2.4:-31300559 None:intergenic
AAATGGTCTACCAAGAAGTT+GGG 0.502052 2.4:+31302389 MS.gene043872:CDS
TTTCGAAGAGCGGGAAAGAT+GGG 0.502201 2.4:+31301659 MS.gene043872:CDS
GTTGGATTCGTCTTCAAAGT+TGG 0.502236 2.4:-31300736 None:intergenic
ATACTTGTCATGGGTGTACT+CGG 0.502362 2.4:-31301919 None:intergenic
TTACCTGGTTGTTCGAAACA+TGG 0.503284 2.4:+31301349 MS.gene043872:CDS
TGGTCATCTTAGAGTGAAAG+AGG 0.503540 2.4:+31302169 MS.gene043872:CDS
TATGTCTTCGAGGCCTCCAA+TGG 0.508338 2.4:+31301380 MS.gene043872:CDS
TCGGTTACAATTCCCATTGG+AGG 0.511504 2.4:-31301393 None:intergenic
TCTGATGGGTTTCAAGCGTC+CGG 0.512219 2.4:+31302758 MS.gene043872:CDS
TTACGAAAATCTAACATTTG+TGG 0.512305 2.4:+31300987 MS.gene043872:CDS
AAACGATCAGGCAGGTGTAC+GGG 0.512319 2.4:+31300924 MS.gene043872:CDS
CGTTCCATGGTATGTGATGC+CGG 0.512631 2.4:+31300961 MS.gene043872:CDS
GAGAGATGTCAGAAACTTTG+TGG 0.514693 2.4:+31301011 MS.gene043872:CDS
CATCTCCATCCTTCCCGAGA+AGG 0.515681 2.4:-31301047 None:intergenic
TCTGGGCTGATGCACGTAGT+AGG 0.516810 2.4:+31301169 MS.gene043872:CDS
CAAAAGAAAGACACCTTCTC+GGG 0.517286 2.4:+31301034 MS.gene043872:CDS
GTATCTCCATATACTTGTCA+TGG 0.517305 2.4:-31301929 None:intergenic
CCTTCAAATCTTTCTATGTG+TGG 0.521556 2.4:-31302284 None:intergenic
ATAACGGTTGCTTTAAAGAA+GGG 0.522275 2.4:+31300784 MS.gene043872:CDS
TTCCCTCATTCTAGCAAAGT+AGG 0.524536 2.4:-31301085 None:intergenic
AGACACCTTCTCGGGAAGGA+TGG 0.525565 2.4:+31301042 MS.gene043872:CDS
GAACAAAAGTTTCCGTTCCA+TGG 0.529264 2.4:+31300948 MS.gene043872:CDS
GGATTCGTCTTCAAAGTTGG+TGG 0.530111 2.4:-31300733 None:intergenic
TAAACGATCAGGCAGGTGTA+CGG 0.533293 2.4:+31300923 MS.gene043872:CDS
AAGTATTGTGTTGATTTCAA+CGG 0.536214 2.4:+31302068 MS.gene043872:CDS
TAAAACCACTAGTTGACTGT+TGG 0.536849 2.4:-31302805 None:intergenic
ATCCTACTTTGCTAGAATGA+GGG 0.537508 2.4:+31301083 MS.gene043872:CDS
TGATCGGTTACAATTCCCAT+TGG 0.541217 2.4:-31301396 None:intergenic
CATCAGCTAGCTCTCCGTCA+GGG 0.541329 2.4:-31300462 None:intergenic
CTGCATAGCCTTGCACTGAT+CGG 0.542185 2.4:-31301412 None:intergenic
CTATGAGATTGACATGGACG+AGG 0.547341 2.4:+31301125 MS.gene043872:CDS
TGTTTGTTGTTTGAGTTCAG+AGG 0.550114 2.4:+31302116 MS.gene043872:CDS
TGATTGCTCAGCTAAGATAA+CGG 0.550952 2.4:+31300768 MS.gene043872:CDS
GATAAATTCACGCCTACTAT+CGG 0.551302 2.4:+31300547 MS.gene043872:CDS
TATCTCCATATACTTGTCAT+GGG 0.551454 2.4:-31301928 None:intergenic
ATTCTAACTTGTGCAAGAGA+AGG 0.552020 2.4:+31300691 MS.gene043872:CDS
AGGTCTGCCTATGAATACTT+TGG 0.552277 2.4:+31301189 MS.gene043872:CDS
CAGCCACATTGTGGTGTGGG+AGG 0.557227 2.4:+31302425 MS.gene043872:CDS
TTACAGCCACATTGTGGTGT+GGG 0.557892 2.4:+31302422 MS.gene043872:CDS
TGTTTCGAACAACCAGGTAA+AGG 0.558836 2.4:-31301346 None:intergenic
AACTCTAAGAAAGGGGCCGA+TGG 0.560180 2.4:+31300652 MS.gene043872:CDS
AGCCACATTGTGGTGTGGGA+GGG 0.562336 2.4:+31302426 MS.gene043872:CDS
CTAAATTTATCTTGCCACGA+TGG 0.563368 2.4:+31302198 MS.gene043872:CDS
GAGATACAAGTAGAATTTCG+TGG 0.566723 2.4:+31301945 MS.gene043872:CDS
GAACAACCAGGTAAAGGACT+TGG 0.570795 2.4:-31301340 None:intergenic
ATGTCTTCGAGGCCTCCAAT+GGG 0.571048 2.4:+31301381 MS.gene043872:CDS
TGTTTCCTAATACTAGGCAT+AGG 0.571109 2.4:+31301451 MS.gene043872:CDS
TTGTTGGTGTCAATCATCAT+GGG 0.576932 2.4:+31301274 MS.gene043872:CDS
TCTCGAAGGTGTGACTACAT+TGG 0.577648 2.4:+31301983 MS.gene043872:CDS
ATCAGCTAGCTCTCCGTCAG+GGG 0.582853 2.4:-31300461 None:intergenic
TCAGACATAGGAAGTAATGT+AGG 0.589454 2.4:-31302740 None:intergenic
TTGTGTGTATGATTCTCTGC+GGG 0.590009 2.4:+31301560 MS.gene043872:CDS
CATTTCTTCATACAAATCAG+AGG 0.596809 2.4:-31302363 None:intergenic
GCTTGAAACCCATCAGACAT+AGG 0.602294 2.4:-31302752 None:intergenic
GTGAATATGCCATGAAACAA+GGG 0.602322 2.4:+31300611 MS.gene043872:CDS
AAGATACCAAGTCCTTTACC+TGG 0.605756 2.4:+31301334 MS.gene043872:CDS
GAAGTAGTTAAGCGTATCAC+CGG 0.605920 2.4:-31302777 None:intergenic
CGACAACGCACTCCAAGACA+AGG 0.606607 2.4:+31301809 MS.gene043872:CDS
AGATACAAGTAGAATTTCGT+GGG 0.607840 2.4:+31301946 MS.gene043872:CDS
CGGGAAGGATGGAGATGGCA+GGG 0.609041 2.4:+31301053 MS.gene043872:CDS
AGAAAGACACCTTCTCGGGA+AGG 0.609091 2.4:+31301038 MS.gene043872:CDS
GACAACCGACGATTAGTTCG+AGG 0.611005 2.4:-31302539 None:intergenic
TGCATCAATGAGCAAAGATG+TGG 0.611914 2.4:+31302838 MS.gene043872:CDS
ACCACCGGCATCACATACCA+TGG 0.613048 2.4:-31300965 None:intergenic
AAATGCAACTGTTATCTCGA+AGG 0.615193 2.4:+31301969 MS.gene043872:CDS
TTCCTAATACTAGGCATAGG+TGG 0.615209 2.4:+31301454 MS.gene043872:CDS
CAAGCACAATCATTCCATGC+GGG 0.616971 2.4:+31301865 MS.gene043872:CDS
GGTTGTTCGAAACATGGCTA+AGG 0.617470 2.4:+31301355 MS.gene043872:CDS
CGTGGCAAGATAAATTTAGA+CGG 0.619992 2.4:-31302194 None:intergenic
AATTGTAACCGATCAGTGCA+AGG 0.620761 2.4:+31301404 MS.gene043872:CDS
TCATCCCAACAGTCAACTAG+TGG 0.623204 2.4:+31302800 MS.gene043872:CDS
CCTGACGGAGAGCTAGCTGA+TGG 0.623440 2.4:+31300463 MS.gene043872:CDS
CCGTTAAAAGTACCACGTAA+GGG 0.624486 2.4:+31302512 MS.gene043872:CDS
CGGCATCACATACCATGGAA+CGG 0.625469 2.4:-31300960 None:intergenic
TAGCCATGTTTCGAACAACC+AGG 0.627145 2.4:-31301352 None:intergenic
ATTGTGGAATTTGACCCGCA+TGG 0.627292 2.4:-31301879 None:intergenic
GTTCGAGGACGTCCCTTACG+TGG 0.628497 2.4:-31302524 None:intergenic
CTTCTCGGGAAGGATGGAGA+TGG 0.631777 2.4:+31301048 MS.gene043872:CDS
TTTACAGCCACATTGTGGTG+TGG 0.632168 2.4:+31302421 MS.gene043872:CDS
AACTAGAAACTCTAAGAAAG+GGG 0.633083 2.4:+31300645 MS.gene043872:CDS
ATGAGTTTACAGCCACATTG+TGG 0.634509 2.4:+31302416 MS.gene043872:CDS
AAAGGATACGCACAATACAA+AGG 0.637659 2.4:+31301516 MS.gene043872:CDS
GGATGATCGATCAATATAGG+AGG 0.639611 2.4:-31300430 None:intergenic
TACAAATCAGAGGTTTGACT+AGG 0.642589 2.4:-31302353 None:intergenic
CAAGGCTGAAAAGGAATACG+AGG 0.643480 2.4:+31301827 MS.gene043872:CDS
AGATAAGTCCAACTAAATCA+CGG 0.645312 2.4:+31300848 MS.gene043872:CDS
TCAGCTAGCTCTCCGTCAGG+GGG 0.662037 2.4:-31300460 None:intergenic
TTATGAAATTGCAGAATGTG+CGG 0.662542 2.4:+31302322 MS.gene043872:CDS
CGGAGAGCTAGCTGATGGTG+CGG 0.662675 2.4:+31300468 MS.gene043872:CDS
ATGATTGACACCAACAAACG+GGG 0.665562 2.4:-31301268 None:intergenic
TTAGAGGAGAGTATTGTCTG+TGG 0.669312 2.4:+31302029 MS.gene043872:CDS
GGATCATAGCTTAAACACGA+TGG 0.674970 2.4:+31302448 MS.gene043872:CDS
GACGTCCTCGAACTAATCGT+CGG 0.680634 2.4:+31302534 MS.gene043872:CDS
TCATAGCTTAAACACGATGG+AGG 0.690849 2.4:+31302451 MS.gene043872:CDS
ATACTTGTTTGTCAAGTACG+TGG 0.696002 2.4:-31301232 None:intergenic
TCAAGCACAATCATTCCATG+CGG 0.697666 2.4:+31301864 MS.gene043872:CDS
TCCCCTCCCACACCACAATG+TGG 0.710147 2.4:-31302428 None:intergenic
ATTGTGTGTATGATTCTCTG+CGG 0.733784 2.4:+31301559 MS.gene043872:CDS
TCCATGGTATGTGATGCCGG+TGG 0.764195 2.4:+31300964 MS.gene043872:CDS
CCGGAATGTCAACAACACAG+AGG 0.797075 2.4:+31301712 MS.gene043872:CDS
AGATTGATATGCATGCACAG+CGG 0.798983 2.4:+31300890 MS.gene043872:CDS
GTATTGTGTTGATTTCAACG+GGG 0.841514 2.4:+31302070 MS.gene043872:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAAAAAATGATTTCT+GGG + chr2.4:31301689-31301708 MS.gene043872:CDS 10.0%
!!! AAATCATTTTTTAAATAACA+TGG - chr2.4:31301687-31301706 None:intergenic 10.0%
!! AATGTAATCACAATAATAAT+AGG - chr2.4:31302650-31302669 None:intergenic 15.0%
!! GTTATTTAAAAAATGATTTC+TGG + chr2.4:31301688-31301707 MS.gene043872:CDS 15.0%
!! AAGAAAAAAACCAATTGTTT+CGG + chr2.4:31302601-31302620 MS.gene043872:CDS 20.0%
!! ACAACATTCATTGAAAAATA+TGG + chr2.4:31301606-31301625 MS.gene043872:CDS 20.0%
!! ATTCATTGAAAAATATGGTT+TGG + chr2.4:31301611-31301630 MS.gene043872:CDS 20.0%
!! TTCATTGAAAAATATGGTTT+GGG + chr2.4:31301612-31301631 MS.gene043872:CDS 20.0%
! AAAATTGCCGAAAAATTGAA+AGG + chr2.4:31301498-31301517 MS.gene043872:CDS 25.0%
! TGTATGAAGAAATGATCAAA+TGG + chr2.4:31302372-31302391 MS.gene043872:CDS 25.0%
! TTACGAAAATCTAACATTTG+TGG + chr2.4:31300987-31301006 MS.gene043872:CDS 25.0%
!! AAAACTAGAAACTCTAAGAA+AGG + chr2.4:31300643-31300662 MS.gene043872:CDS 25.0%
!! AAACTAGAAACTCTAAGAAA+GGG + chr2.4:31300644-31300663 MS.gene043872:CDS 25.0%
!! TATCGGTATGTCTTTTAAAT+CGG + chr2.4:31300564-31300583 MS.gene043872:CDS 25.0%
!!! AAGTATTGTGTTGATTTCAA+CGG + chr2.4:31302068-31302087 MS.gene043872:CDS 25.0%
!!! ACTTTTAACGGATTAAGAAT+TGG - chr2.4:31302503-31302522 None:intergenic 25.0%
!!! GAAATATTTTAACATGCCAT+CGG - chr2.4:31300671-31300690 None:intergenic 25.0%
!!! TTTTTTCACAATCTTTTCAG+TGG - chr2.4:31302567-31302586 None:intergenic 25.0%
AAAGGATGATCGATCAATAT+AGG - chr2.4:31300436-31300455 None:intergenic 30.0%
AAGATGACCAAGTACAAAAA+GGG - chr2.4:31302159-31302178 None:intergenic 30.0%
ACACAATACTTAGTTTCGTT+CGG - chr2.4:31302059-31302078 None:intergenic 30.0%
AGATAAGTCCAACTAAATCA+CGG + chr2.4:31300848-31300867 MS.gene043872:CDS 30.0%
AGATACAAGTAGAATTTCGT+GGG + chr2.4:31301946-31301965 MS.gene043872:CDS 30.0%
CATTTCTTCATACAAATCAG+AGG - chr2.4:31302366-31302385 None:intergenic 30.0%
TAAGATGACCAAGTACAAAA+AGG - chr2.4:31302160-31302179 None:intergenic 30.0%
TATCTCCATATACTTGTCAT+GGG - chr2.4:31301931-31301950 None:intergenic 30.0%
TTAAAAGACATACCGATAGT+AGG - chr2.4:31300562-31300581 None:intergenic 30.0%
TTATGAAATTGCAGAATGTG+CGG + chr2.4:31302322-31302341 MS.gene043872:CDS 30.0%
! AACATCACCAAAGTATTCAT+AGG - chr2.4:31301199-31301218 None:intergenic 30.0%
! AACTAGAAACTCTAAGAAAG+GGG + chr2.4:31300645-31300664 MS.gene043872:CDS 30.0%
! ATCTACATACACCATTTTAG+AGG + chr2.4:31302013-31302032 MS.gene043872:CDS 30.0%
! CTTTTTCTATGAGATTGACA+TGG + chr2.4:31301119-31301138 MS.gene043872:CDS 30.0%
! TCATGTGCAGAATATTTTCT+GGG + chr2.4:31301152-31301171 MS.gene043872:CDS 30.0%
! TGCGTATCCTTTCAATTTTT+CGG - chr2.4:31301508-31301527 None:intergenic 30.0%
! TTAAAAAATGATTTCTGGGC+CGG + chr2.4:31301693-31301712 MS.gene043872:CDS 30.0%
! TTCATGTGCAGAATATTTTC+TGG + chr2.4:31301151-31301170 MS.gene043872:CDS 30.0%
!! AGTATTGTGTTGATTTCAAC+GGG + chr2.4:31302069-31302088 MS.gene043872:CDS 30.0%
!! ATAACGGTTGCTTTAAAGAA+GGG + chr2.4:31300784-31300803 MS.gene043872:CDS 30.0%
!! GACAATTTTGATAAACGATC+AGG + chr2.4:31300912-31300931 MS.gene043872:CDS 30.0%
!!! AGTATGAATGCTTTTTTCGA+TGG + chr2.4:31301741-31301760 MS.gene043872:CDS 30.0%
!!! TATGTGTGGTTGTAGTTTTT+TGG - chr2.4:31302273-31302292 None:intergenic 30.0%
AAAACCACTAGTTGACTGTT+GGG - chr2.4:31302807-31302826 None:intergenic 35.0%
AAAGGATACGCACAATACAA+AGG + chr2.4:31301516-31301535 MS.gene043872:CDS 35.0%
AAATGCAACTGTTATCTCGA+AGG + chr2.4:31301969-31301988 MS.gene043872:CDS 35.0%
AAATGGTCTACCAAGAAGTT+GGG + chr2.4:31302389-31302408 MS.gene043872:CDS 35.0%
ACATTACTTCCTATGTCTGA+TGG + chr2.4:31302743-31302762 MS.gene043872:CDS 35.0%
AGGTTGTGTTTCCTAATACT+AGG + chr2.4:31301445-31301464 MS.gene043872:CDS 35.0%
ATACTTGTTTGTCAAGTACG+TGG - chr2.4:31301235-31301254 None:intergenic 35.0%
ATCCTACTTTGCTAGAATGA+GGG + chr2.4:31301083-31301102 MS.gene043872:CDS 35.0%
ATTGTGTGTATGATTCTCTG+CGG + chr2.4:31301559-31301578 MS.gene043872:CDS 35.0%
CATTACTTCCTATGTCTGAT+GGG + chr2.4:31302744-31302763 MS.gene043872:CDS 35.0%
CCACACATAGAAAGATTTGA+AGG + chr2.4:31302284-31302303 MS.gene043872:CDS 35.0%
CCTTCAAATCTTTCTATGTG+TGG - chr2.4:31302287-31302306 None:intergenic 35.0%
CGTGGCAAGATAAATTTAGA+CGG - chr2.4:31302197-31302216 None:intergenic 35.0%
CTAAATTTATCTTGCCACGA+TGG + chr2.4:31302198-31302217 MS.gene043872:CDS 35.0%
CTTGTTTCATGGCATATTCA+CGG - chr2.4:31300612-31300631 None:intergenic 35.0%
GAGATACAAGTAGAATTTCG+TGG + chr2.4:31301945-31301964 MS.gene043872:CDS 35.0%
GATAAATTCACGCCTACTAT+CGG + chr2.4:31300547-31300566 MS.gene043872:CDS 35.0%
GGATTAAGAATTGGTTGATC+AGG - chr2.4:31302494-31302513 None:intergenic 35.0%
GTATCTCCATATACTTGTCA+TGG - chr2.4:31301932-31301951 None:intergenic 35.0%
GTGAATATGCCATGAAACAA+GGG + chr2.4:31300611-31300630 MS.gene043872:CDS 35.0%
TAAAACCACTAGTTGACTGT+TGG - chr2.4:31302808-31302827 None:intergenic 35.0%
TACAAATCAGAGGTTTGACT+AGG - chr2.4:31302356-31302375 None:intergenic 35.0%
TATCCTACTTTGCTAGAATG+AGG + chr2.4:31301082-31301101 MS.gene043872:CDS 35.0%
TCAGACATAGGAAGTAATGT+AGG - chr2.4:31302743-31302762 None:intergenic 35.0%
TGATGATTGACACCAACAAA+CGG - chr2.4:31301273-31301292 None:intergenic 35.0%
TGATTGCTCAGCTAAGATAA+CGG + chr2.4:31300768-31300787 MS.gene043872:CDS 35.0%
TGTTTCCTAATACTAGGCAT+AGG + chr2.4:31301451-31301470 MS.gene043872:CDS 35.0%
TTGAAATTGTCTCTCGATTG+TGG - chr2.4:31301898-31301917 None:intergenic 35.0%
! ATGGTTTGGGTGAAAATGAT+TGG + chr2.4:31301625-31301644 MS.gene043872:CDS 35.0%
! ATTCTAACTTGTGCAAGAGA+AGG + chr2.4:31300691-31300710 MS.gene043872:CDS 35.0%
! CAATCCATTTTACTAGGTTG+CGG + chr2.4:31301297-31301316 MS.gene043872:CDS 35.0%
! GACAATACTCTCCTCTAAAA+TGG - chr2.4:31302027-31302046 None:intergenic 35.0%
! TGAATACCATTTTCGAAGAG+CGG + chr2.4:31301649-31301668 MS.gene043872:CDS 35.0%
! TGTTAGATTTTCGTAACCAC+CGG - chr2.4:31300983-31301002 None:intergenic 35.0%
! TTCACATTTTTACTCCATCG+TGG - chr2.4:31302215-31302234 None:intergenic 35.0%
! TTGGTGGTATGTTTGATTCA+TGG - chr2.4:31300720-31300739 None:intergenic 35.0%
! TTTCACAATCTTTTCAGTGG+TGG - chr2.4:31302564-31302583 None:intergenic 35.0%
!! ATTTGTTGTTTCAAAGGTCC+CGG - chr2.4:31300515-31300534 None:intergenic 35.0%
!! ATTTTGATAAACGATCAGGC+AGG + chr2.4:31300916-31300935 MS.gene043872:CDS 35.0%
!! GACAGCATTTGTTGTTTCAA+AGG - chr2.4:31300521-31300540 None:intergenic 35.0%
!! GATAACGGTTGCTTTAAAGA+AGG + chr2.4:31300783-31300802 MS.gene043872:CDS 35.0%
!! GTATTGTGTTGATTTCAACG+GGG + chr2.4:31302070-31302089 MS.gene043872:CDS 35.0%
!! TGTTTGTTGTTTGAGTTCAG+AGG + chr2.4:31302116-31302135 MS.gene043872:CDS 35.0%
!! TTGCTTTAAAGAAGGGTCTA+TGG + chr2.4:31300791-31300810 MS.gene043872:CDS 35.0%
!! TTGTTGGTGTCAATCATCAT+GGG + chr2.4:31301274-31301293 MS.gene043872:CDS 35.0%
!! TTTGTTGGTGTCAATCATCA+TGG + chr2.4:31301273-31301292 MS.gene043872:CDS 35.0%
!!! GGGTTGATGATTTTGTTGTT+GGG + chr2.4:31301580-31301599 MS.gene043872:CDS 35.0%
!!! TTGATGATTTTGTTGTTGGG+TGG + chr2.4:31301583-31301602 MS.gene043872:CDS 35.0%
AAGACCGCAACCTAGTAAAA+TGG - chr2.4:31301304-31301323 None:intergenic 40.0%
AAGATACCAAGTCCTTTACC+TGG + chr2.4:31301334-31301353 MS.gene043872:CDS 40.0%
AATTGTAACCGATCAGTGCA+AGG + chr2.4:31301404-31301423 MS.gene043872:CDS 40.0%
ACTAAATCACGGTTGTTTGC+TGG + chr2.4:31300859-31300878 MS.gene043872:CDS 40.0%
ACTTCGACAACCGAAACAAT+TGG - chr2.4:31302614-31302633 None:intergenic 40.0%
AGATTGATATGCATGCACAG+CGG + chr2.4:31300890-31300909 MS.gene043872:CDS 40.0%
AGGTCTGCCTATGAATACTT+TGG + chr2.4:31301189-31301208 MS.gene043872:CDS 40.0%
ATACTTGTCATGGGTGTACT+CGG - chr2.4:31301922-31301941 None:intergenic 40.0%
ATGAGTTTACAGCCACATTG+TGG + chr2.4:31302416-31302435 MS.gene043872:CDS 40.0%
ATGATTGACACCAACAAACG+GGG - chr2.4:31301271-31301290 None:intergenic 40.0%
CAAAAGAAAGACACCTTCTC+GGG + chr2.4:31301034-31301053 MS.gene043872:CDS 40.0%
CAAATGGTCTACCAAGAAGT+TGG + chr2.4:31302388-31302407 MS.gene043872:CDS 40.0%
CGTGAATATGCCATGAAACA+AGG + chr2.4:31300610-31300629 MS.gene043872:CDS 40.0%
GAACAAAAGTTTCCGTTCCA+TGG + chr2.4:31300948-31300967 MS.gene043872:CDS 40.0%
GAAGTAGTTAAGCGTATCAC+CGG - chr2.4:31302780-31302799 None:intergenic 40.0%
GAGAGATGTCAGAAACTTTG+TGG + chr2.4:31301011-31301030 MS.gene043872:CDS 40.0%
GAGCAATCAAGTTTCTTCGT+TGG - chr2.4:31300757-31300776 None:intergenic 40.0%
GATGATTGACACCAACAAAC+GGG - chr2.4:31301272-31301291 None:intergenic 40.0%
GCAAAAGAAAGACACCTTCT+CGG + chr2.4:31301033-31301052 MS.gene043872:CDS 40.0%
GCAAACAACCGTGATTTAGT+TGG - chr2.4:31300859-31300878 None:intergenic 40.0%
GGATCATAGCTTAAACACGA+TGG + chr2.4:31302448-31302467 MS.gene043872:CDS 40.0%
GGATGATCGATCAATATAGG+AGG - chr2.4:31300433-31300452 None:intergenic 40.0%
GTACACCCATGACAAGTATA+TGG + chr2.4:31301923-31301942 MS.gene043872:CDS 40.0%
GTTGGATTCGTCTTCAAAGT+TGG - chr2.4:31300739-31300758 None:intergenic 40.0%
TCAAGCACAATCATTCCATG+CGG + chr2.4:31301864-31301883 MS.gene043872:CDS 40.0%
TCATAGCTTAAACACGATGG+AGG + chr2.4:31302451-31302470 MS.gene043872:CDS 40.0%
TGATCGGTTACAATTCCCAT+TGG - chr2.4:31301399-31301418 None:intergenic 40.0%
TGCATCAATGAGCAAAGATG+TGG + chr2.4:31302838-31302857 MS.gene043872:CDS 40.0%
TGGTCATCTTAGAGTGAAAG+AGG + chr2.4:31302169-31302188 MS.gene043872:CDS 40.0%
TGTGCTTGAAGACCGAAAAT+CGG - chr2.4:31301853-31301872 None:intergenic 40.0%
TGTTTCGAACAACCAGGTAA+AGG - chr2.4:31301349-31301368 None:intergenic 40.0%
TTACCTGGTTGTTCGAAACA+TGG + chr2.4:31301349-31301368 MS.gene043872:CDS 40.0%
TTCCCTCATTCTAGCAAAGT+AGG - chr2.4:31301088-31301107 None:intergenic 40.0%
TTCCTAATACTAGGCATAGG+TGG + chr2.4:31301454-31301473 MS.gene043872:CDS 40.0%
TTGTGTGTATGATTCTCTGC+GGG + chr2.4:31301560-31301579 MS.gene043872:CDS 40.0%
TTTCGGTTGTCGAAGTAAGT+TGG + chr2.4:31302618-31302637 MS.gene043872:intron 40.0%
! ACTCATTTTGCCCAACTTCT+TGG - chr2.4:31302402-31302421 None:intergenic 40.0%
! AGACATGCCCTTTTTGTACT+TGG + chr2.4:31302149-31302168 MS.gene043872:CDS 40.0%
! CATGGGCAATCCATTTTACT+AGG + chr2.4:31301291-31301310 MS.gene043872:CDS 40.0%
! CCCTTACGTGGTACTTTTAA+CGG - chr2.4:31302515-31302534 None:intergenic 40.0%
! GAATACCATTTTCGAAGAGC+GGG + chr2.4:31301650-31301669 MS.gene043872:CDS 40.0%
!! ATTCCTTTTCAGCCTTGTCT+TGG - chr2.4:31301824-31301843 None:intergenic 40.0%
!! CCGTTAAAAGTACCACGTAA+GGG + chr2.4:31302512-31302531 MS.gene043872:CDS 40.0%
!! TCCGTTAAAAGTACCACGTA+AGG + chr2.4:31302511-31302530 MS.gene043872:CDS 40.0%
!! TTAGAGGAGAGTATTGTCTG+TGG + chr2.4:31302029-31302048 MS.gene043872:CDS 40.0%
!!! CGGGTTGATGATTTTGTTGT+TGG + chr2.4:31301579-31301598 MS.gene043872:CDS 40.0%
ACTCCAAGACAAGGCTGAAA+AGG + chr2.4:31301818-31301837 MS.gene043872:CDS 45.0%
ATTGTGGAATTTGACCCGCA+TGG - chr2.4:31301882-31301901 None:intergenic 45.0%
CAAGCACAATCATTCCATGC+GGG + chr2.4:31301865-31301884 MS.gene043872:CDS 45.0%
CAAGGCTGAAAAGGAATACG+AGG + chr2.4:31301827-31301846 MS.gene043872:CDS 45.0%
CACCACCTATGCCTAGTATT+AGG - chr2.4:31301459-31301478 None:intergenic 45.0%
CATCCTTTATTACCCCCTGA+CGG + chr2.4:31300448-31300467 MS.gene043872:CDS 45.0%
CTATGAGATTGACATGGACG+AGG + chr2.4:31301125-31301144 MS.gene043872:CDS 45.0%
GAACAACCAGGTAAAGGACT+TGG - chr2.4:31301343-31301362 None:intergenic 45.0%
GCTTGAAACCCATCAGACAT+AGG - chr2.4:31302755-31302774 None:intergenic 45.0%
GGATTCGTCTTCAAAGTTGG+TGG - chr2.4:31300736-31300755 None:intergenic 45.0%
GGCTATGCAGAATGCAATTC+AGG + chr2.4:31301425-31301444 MS.gene043872:CDS 45.0%
GGTTGTTCGAAACATGGCTA+AGG + chr2.4:31301355-31301374 MS.gene043872:CDS 45.0%
TAAACGATCAGGCAGGTGTA+CGG + chr2.4:31300923-31300942 MS.gene043872:CDS 45.0%
TAGCCATGTTTCGAACAACC+AGG - chr2.4:31301355-31301374 None:intergenic 45.0%
TCATCCCAACAGTCAACTAG+TGG + chr2.4:31302800-31302819 MS.gene043872:CDS 45.0%
TCGGTTACAATTCCCATTGG+AGG - chr2.4:31301396-31301415 None:intergenic 45.0%
TCTTTCCCGCTCTTCGAAAA+TGG - chr2.4:31301658-31301677 None:intergenic 45.0%
TTACAGCCACATTGTGGTGT+GGG + chr2.4:31302422-31302441 MS.gene043872:CDS 45.0%
TTTACAGCCACATTGTGGTG+TGG + chr2.4:31302421-31302440 MS.gene043872:CDS 45.0%
TTTCGAAGAGCGGGAAAGAT+GGG + chr2.4:31301659-31301678 MS.gene043872:CDS 45.0%
! CCTCTGTGTTGTTGACATTC+CGG - chr2.4:31301715-31301734 None:intergenic 45.0%
! GATCGCAAACCCTTGTTTCA+TGG - chr2.4:31300623-31300642 None:intergenic 45.0%
! TCTCGAAGGTGTGACTACAT+TGG + chr2.4:31301983-31302002 MS.gene043872:CDS 45.0%
! TTTTCGAAGAGCGGGAAAGA+TGG + chr2.4:31301658-31301677 MS.gene043872:CDS 45.0%
!! AGGAATACGAGGCCGATTTT+CGG + chr2.4:31301838-31301857 MS.gene043872:CDS 45.0%
AAACGATCAGGCAGGTGTAC+GGG + chr2.4:31300924-31300943 MS.gene043872:CDS 50.0%
ACACCAACAAACGGGGCAAA+TGG - chr2.4:31301264-31301283 None:intergenic 50.0%
AGAAAGACACCTTCTCGGGA+AGG + chr2.4:31301038-31301057 MS.gene043872:CDS 50.0%
ATGTCTTCGAGGCCTCCAAT+GGG + chr2.4:31301381-31301400 MS.gene043872:CDS 50.0%
CCGGAATGTCAACAACACAG+AGG + chr2.4:31301712-31301731 MS.gene043872:CDS 50.0%
CGGCATCACATACCATGGAA+CGG - chr2.4:31300963-31300982 None:intergenic 50.0%
CGTTCCATGGTATGTGATGC+CGG + chr2.4:31300961-31300980 MS.gene043872:CDS 50.0%
CTAGGCATAGGTGGTGTCTA+TGG + chr2.4:31301463-31301482 MS.gene043872:CDS 50.0%
GACGTCCTCGAACTAATCGT+CGG + chr2.4:31302534-31302553 MS.gene043872:CDS 50.0%
GGCTAAGGTGTATGTCTTCG+AGG + chr2.4:31301370-31301389 MS.gene043872:CDS 50.0%
TATGTCTTCGAGGCCTCCAA+TGG + chr2.4:31301380-31301399 MS.gene043872:CDS 50.0%
TCTCCGTCAGGGGGTAATAA+AGG - chr2.4:31300454-31300473 None:intergenic 50.0%
! AACTCTAAGAAAGGGGCCGA+TGG + chr2.4:31300652-31300671 MS.gene043872:CDS 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG + chr2.4:31301258-31301277 MS.gene043872:CDS 50.0%
! CGTGTTGACGTTTCGGATTC+CGG + chr2.4:31300493-31300512 MS.gene043872:CDS 50.0%
! CTGCATAGCCTTGCACTGAT+CGG - chr2.4:31301415-31301434 None:intergenic 50.0%
! GACAACCGACGATTAGTTCG+AGG - chr2.4:31302542-31302561 None:intergenic 50.0%
! GTGTTGACGTTTCGGATTCC+GGG + chr2.4:31300494-31300513 MS.gene043872:CDS 50.0%
!! TCTGATGGGTTTCAAGCGTC+CGG + chr2.4:31302758-31302777 MS.gene043872:CDS 50.0%
ACCACCGGCATCACATACCA+TGG - chr2.4:31300968-31300987 None:intergenic 55.0%
AGACACCTTCTCGGGAAGGA+TGG + chr2.4:31301042-31301061 MS.gene043872:CDS 55.0%
AGCCACATTGTGGTGTGGGA+GGG + chr2.4:31302426-31302445 MS.gene043872:CDS 55.0%
CATCTCCATCCTTCCCGAGA+AGG - chr2.4:31301050-31301069 None:intergenic 55.0%
CGACAACGCACTCCAAGACA+AGG + chr2.4:31301809-31301828 MS.gene043872:CDS 55.0%
CTTCTCGGGAAGGATGGAGA+TGG + chr2.4:31301048-31301067 MS.gene043872:CDS 55.0%
TCCATGGTATGTGATGCCGG+TGG + chr2.4:31300964-31300983 MS.gene043872:CDS 55.0%
TCTGGGCTGATGCACGTAGT+AGG + chr2.4:31301169-31301188 MS.gene043872:CDS 55.0%
! ATCAGCTAGCTCTCCGTCAG+GGG - chr2.4:31300464-31300483 None:intergenic 55.0%
! CATCAGCTAGCTCTCCGTCA+GGG - chr2.4:31300465-31300484 None:intergenic 55.0%
! TGCGGCTCGTGTTGACGTTT+CGG + chr2.4:31300486-31300505 MS.gene043872:CDS 55.0%
CAGCCACATTGTGGTGTGGG+AGG + chr2.4:31302425-31302444 MS.gene043872:CDS 60.0%
CCTGACGGAGAGCTAGCTGA+TGG + chr2.4:31300463-31300482 MS.gene043872:CDS 60.0%
CGGGAAGGATGGAGATGGCA+GGG + chr2.4:31301053-31301072 MS.gene043872:CDS 60.0%
GCCACATTGTGGTGTGGGAG+GGG + chr2.4:31302427-31302446 MS.gene043872:CDS 60.0%
GTTCGAGGACGTCCCTTACG+TGG - chr2.4:31302527-31302546 None:intergenic 60.0%
TCCCCTCCCACACCACAATG+TGG - chr2.4:31302431-31302450 None:intergenic 60.0%
TCGGGAAGGATGGAGATGGC+AGG + chr2.4:31301052-31301071 MS.gene043872:CDS 60.0%
! CCATCAGCTAGCTCTCCGTC+AGG - chr2.4:31300466-31300485 None:intergenic 60.0%
! CGGAGAGCTAGCTGATGGTG+CGG + chr2.4:31300468-31300487 MS.gene043872:CDS 60.0%
! TCAGCTAGCTCTCCGTCAGG+GGG - chr2.4:31300463-31300482 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 31300418 31302877 31300418 ID=MS.gene043872
chr2.4 mRNA 31300418 31302877 31300418 ID=MS.gene043872.t1;Parent=MS.gene043872
chr2.4 exon 31300418 31302631 31300418 ID=MS.gene043872.t1.exon1;Parent=MS.gene043872.t1
chr2.4 CDS 31300418 31302631 31300418 ID=cds.MS.gene043872.t1;Parent=MS.gene043872.t1
chr2.4 exon 31302740 31302877 31302740 ID=MS.gene043872.t1.exon2;Parent=MS.gene043872.t1
chr2.4 CDS 31302740 31302877 31302740 ID=cds.MS.gene043872.t1;Parent=MS.gene043872.t1
Gene Sequence

>MS.gene043872

ATGGCTGATGGTCCTCCTATATTGATCGATCATCCTTTATTACCCCCTGACGGAGAGCTAGCTGATGGTGCGGCTCGTGTTGACGTTTCGGATTCCGGGACCTTTGAAACAACAAATGCTGTCGCAACAGATAAATTCACGCCTACTATCGGTATGTCTTTTAAATCGGTTGCAGATTGTAAGTCTTTTTACCGTGAATATGCCATGAAACAAGGGTTTGCGATCAAAACTAGAAACTCTAAGAAAGGGGCCGATGGCATGTTAAAATATTTCATTCTAACTTGTGCAAGAGAAGGTCACCATGAATCAAACATACCACCAACTTTGAAGACGAATCCAACGAAGAAACTTGATTGCTCAGCTAAGATAACGGTTGCTTTAAAGAAGGGTCTATGGACTATTTTGTCTTTTAATTCATGTCATAATCATGAGATAAGTCCAACTAAATCACGGTTGTTTGCTGGCAATAAGAAGATTGATATGCATGCACAGCGGACAATTTTGATAAACGATCAGGCAGGTGTACGGGTGAACAAAAGTTTCCGTTCCATGGTATGTGATGCCGGTGGTTACGAAAATCTAACATTTGTGGAGAGAGATGTCAGAAACTTTGTGGCAAAAGAAAGACACCTTCTCGGGAAGGATGGAGATGGCAGGGCACTTTTATCCTACTTTGCTAGAATGAGGGAATGCAACAAAGACTTTTTCTATGAGATTGACATGGACGAGGACTTTCATGTGCAGAATATTTTCTGGGCTGATGCACGTAGTAGGTCTGCCTATGAATACTTTGGTGATGTTGTCACATTCGATACCACGTACTTGACAAACAAGTATGACATGCCATTTGCCCCGTTTGTTGGTGTCAATCATCATGGGCAATCCATTTTACTAGGTTGCGGTCTTTTATCATCTGAAGATACCAAGTCCTTTACCTGGTTGTTCGAAACATGGCTAAGGTGTATGTCTTCGAGGCCTCCAATGGGAATTGTAACCGATCAGTGCAAGGCTATGCAGAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGAAAAATTGCCGAAAAATTGAAAGGATACGCACAATACAAAGGAATAAAAAATGATATGAAAAATTGTGTGTATGATTCTCTGCGGGTTGATGATTTTGTTGTTGGGTGGACAACATTCATTGAAAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAAAATGATTTCTGGGCCGGAATGTCAACAACACAGAGGAGTGAAAGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTAAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCCGATTTTCGGTCTTCAAGCACAATCATTCCATGCGGGTCAAATTCCACAATCGAGAGACAATTTCAAGCCGAGTACACCCATGACAAGTATATGGAGATACAAGTAGAATTTCGTGGGAAATGCAACTGTTATCTCGAAGGTGTGACTACATTGGAATTTACATCTACATACACCATTTTAGAGGAGAGTATTGTCTGTGGTAAACCGAACGAAACTAAGTATTGTGTTGATTTCAACGGGGAAGACAATAATGTTACGTGCACTTGTTTGTTGTTTGAGTTCAGAGGCATTTTGTGTAGACATGCCCTTTTTGTACTTGGTCATCTTAGAGTGAAAGAGGTTCCGTCTAAATTTATCTTGCCACGATGGAGTAAAAATGTGAAGAGAAAATATGCACACATGAAGACAAGTTACAATGCCAAAAAACTACAACCACACATAGAAAGATTTGAAGGTCTTTGCAAGACATTTTATGAAATTGCAGAATGTGCGGCTGAGACACCTAGTCAAACCTCTGATTTGTATGAAGAAATGATCAAATGGTCTACCAAGAAGTTGGGCAAAATGAGTTTACAGCCACATTGTGGTGTGGGAGGGGATCATAGCTTAAACACGATGGAGGACTCATTATCACAGCAACCTGATCAACCAATTCTTAATCCGTTAAAAGTACCACGTAAGGGACGTCCTCGAACTAATCGTCGGTTGTCCACCACTGAAAAGATTGTGAAAAAAAGAAGCAAAATGCAAAGAAAAAAACCAATTGTTTCGGTTGTCGAAGTAAGTTGGTGTTAACCTATTATTATTGTGATTACATTTGTTTTTTTAATAATAATACAATGTTAATTATGTTAAATGTAATTAATATTTCACTCTGTAATACTACAGCCTACATTACTTCCTATGTCTGATGGGTTTCAAGCGTCCGGTGATACGCTTAACTACTTCTCATCCCAACAGTCAACTAGTGGTTTTATGTCATTACTTGCATCAATGAGCAAAGATGTGGACGCAGCTGAATTTTAA
Protein sequence

>MS.gene043872.t1

MADGPPILIDHPLLPPDGELADGAARVDVSDSGTFETTNAVATDKFTPTIGMSFKSVADCKSFYREYAMKQGFAIKTRNSKKGADGMLKYFILTCAREGHHESNIPPTLKTNPTKKLDCSAKITVALKKGLWTILSFNSCHNHEISPTKSRLFAGNKKIDMHAQRTILINDQAGVRVNKSFRSMVCDAGGYENLTFVERDVRNFVAKERHLLGKDGDGRALLSYFARMRECNKDFFYEIDMDEDFHVQNIFWADARSRSAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSILLGCGLLSSEDTKSFTWLFETWLRCMSSRPPMGIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKIAEKLKGYAQYKGIKNDMKNCVYDSLRVDDFVVGWTTFIEKYGLGENDWLNTIFEERERWVPCYLKNDFWAGMSTTQRSESMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPCGSNSTIERQFQAEYTHDKYMEIQVEFRGKCNCYLEGVTTLEFTSTYTILEESIVCGKPNETKYCVDFNGEDNNVTCTCLLFEFRGILCRHALFVLGHLRVKEVPSKFILPRWSKNVKRKYAHMKTSYNAKKLQPHIERFEGLCKTFYEIAECAAETPSQTSDLYEEMIKWSTKKLGKMSLQPHCGVGGDHSLNTMEDSLSQQPDQPILNPLKVPRKGRPRTNRRLSTTEKIVKKRSKMQRKKPIVSVVEPTLLPMSDGFQASGDTLNYFSSQQSTSGFMSLLASMSKDVDAAEF