AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043885

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043885.t1 MTR_5g065200 91.211 842 58 6 1 831 1 837 0.0 1507
MS.gene043885.t1 MTR_1g114150 70.596 806 197 9 45 830 267 1052 0.0 1153
MS.gene043885.t1 MTR_1g114130 72.813 743 179 6 106 829 301 1039 0.0 1105
MS.gene043885.t1 MTR_1g069565 62.822 893 257 9 3 830 1 883 0.0 1073
MS.gene043885.t1 MTR_1g114070 70.225 712 205 2 125 830 216 926 0.0 1033
MS.gene043885.t1 MTR_1g069565 57.474 950 272 12 3 830 1 940 0.0 1004
MS.gene043885.t1 MTR_1g114150 67.244 693 187 9 45 717 267 939 0.0 937
MS.gene043885.t1 MTR_5g047620 65.809 661 183 9 205 829 145 798 0.0 864
MS.gene043885.t1 MTR_0020s0100 61.514 621 209 11 205 811 87 691 0.0 753
MS.gene043885.t1 MTR_2g024270 45.916 808 380 14 33 826 53 817 0.0 632
MS.gene043885.t1 MTR_4g132540 45.308 746 320 13 127 826 161 864 0.0 618
MS.gene043885.t1 MTR_2g024140 47.578 702 323 10 127 826 27 685 0.0 612
MS.gene043885.t1 MTR_7g117445 38.566 809 367 21 130 827 136 925 4.18e-166 506
MS.gene043885.t1 MTR_7g117445 38.566 809 367 21 130 827 136 925 6.28e-166 505
MS.gene043885.t1 MTR_7g117445 38.566 809 367 21 130 827 136 925 1.51e-165 506
MS.gene043885.t1 MTR_7g117445 43.793 580 272 10 130 683 136 687 1.02e-150 464
MS.gene043885.t1 MTR_1g083120 35.762 741 383 12 134 827 198 892 2.47e-148 459
MS.gene043885.t1 MTR_1g083120 35.762 741 383 12 134 827 215 909 5.84e-148 459
MS.gene043885.t1 MTR_1g083120 34.993 683 351 12 134 769 198 834 1.43e-129 408
MS.gene043885.t1 MTR_1g083120 34.993 683 351 12 134 769 198 834 2.88e-129 407
MS.gene043885.t1 MTR_1g008060 44.527 539 256 12 128 659 855 1357 3.33e-129 424
MS.gene043885.t1 MTR_1g008060 74.265 136 35 0 682 817 1542 1677 4.87e-58 216
MS.gene043885.t1 MTR_1g008060 44.527 539 256 12 128 659 864 1366 3.76e-129 424
MS.gene043885.t1 MTR_1g008060 74.265 136 35 0 682 817 1551 1686 4.93e-58 216
MS.gene043885.t1 MTR_8g062940 49.315 365 153 4 134 498 33 365 1.97e-109 340
MS.gene043885.t1 MTR_2g024240 56.352 307 120 3 489 791 92 388 3.13e-109 340
MS.gene043885.t1 MTR_2g011630 34.286 525 321 10 134 649 207 716 3.56e-84 289
MS.gene043885.t1 MTR_2g011630 48.993 149 76 0 682 830 810 958 5.73e-40 159
MS.gene043885.t1 MTR_2g011630 34.615 442 266 9 217 649 45 472 1.69e-70 247
MS.gene043885.t1 MTR_2g011630 48.993 149 76 0 682 830 566 714 2.69e-40 159
MS.gene043885.t1 MTR_2g011630 34.615 442 266 9 217 649 77 504 2.77e-70 247
MS.gene043885.t1 MTR_2g011630 48.993 149 76 0 682 830 598 746 3.53e-40 159
MS.gene043885.t1 MTR_0295s0020 62.424 165 62 0 502 666 12 176 2.46e-61 205
MS.gene043885.t1 MTR_4g091520 39.057 297 170 5 357 649 657 946 1.83e-59 220
MS.gene043885.t1 MTR_4g091520 35.025 197 120 4 106 295 230 425 1.46e-28 124
MS.gene043885.t1 MTR_4g091520 39.057 297 170 5 357 649 692 981 1.96e-59 220
MS.gene043885.t1 MTR_4g091520 35.025 197 120 4 106 295 230 425 1.44e-28 124
MS.gene043885.t1 MTR_4g091520 39.057 297 170 5 357 649 727 1016 2.48e-59 220
MS.gene043885.t1 MTR_4g091520 53.378 148 65 2 681 825 1132 1278 1.13e-42 169
MS.gene043885.t1 MTR_4g091520 35.025 197 120 4 106 295 230 425 2.05e-28 123
MS.gene043885.t1 MTR_4g091520 39.057 297 170 5 357 649 727 1016 2.61e-59 220
MS.gene043885.t1 MTR_4g091520 35.025 197 120 4 106 295 230 425 1.48e-28 124
MS.gene043885.t1 MTR_8g465700 52.778 180 81 1 444 623 2 177 2.36e-55 192
MS.gene043885.t1 MTR_8g465720 70.526 95 27 1 675 769 46 139 1.66e-40 147
MS.gene043885.t1 MTR_8g013920 66.165 133 26 3 536 668 40 153 2.50e-39 144
MS.gene043885.t1 MTR_3g080750 71.739 46 13 0 623 668 86 131 2.45e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043885.t1 AT3G07610 45.602 864 391 13 29 830 49 895 0.0 702
MS.gene043885.t1 AT3G07610 44.507 883 395 14 29 830 49 917 0.0 681
MS.gene043885.t1 AT1G11950 49.286 700 332 7 130 826 189 868 0.0 676
MS.gene043885.t1 AT1G11950 49.286 700 332 7 130 826 189 868 0.0 676
MS.gene043885.t1 AT1G62310 49.130 690 320 11 133 810 208 878 0.0 652
MS.gene043885.t1 AT1G11950 47.077 633 312 7 130 759 189 801 0.0 572
MS.gene043885.t1 AT3G07610 41.489 658 312 12 29 629 49 690 1.82e-148 455
MS.gene043885.t1 AT4G00990 42.274 563 288 12 134 685 80 616 1.17e-133 417
MS.gene043885.t1 AT4G00990 61.062 113 39 2 663 775 671 778 7.49e-35 143
MS.gene043885.t1 AT4G00990 42.274 563 288 12 134 685 80 616 1.40e-133 417
MS.gene043885.t1 AT4G00990 64.762 105 32 2 663 767 671 770 4.66e-34 140
MS.gene043885.t1 AT4G00990 42.274 563 288 12 134 685 80 616 2.50e-133 418
MS.gene043885.t1 AT4G00990 61.963 163 57 2 663 825 671 828 1.15e-56 209
MS.gene043885.t1 AT1G09060 32.440 746 408 15 127 827 197 891 1.22e-120 387
MS.gene043885.t1 AT1G09060 32.440 746 408 15 127 827 197 891 1.22e-120 387
MS.gene043885.t1 AT1G09060 32.440 746 408 15 127 827 211 905 1.99e-120 387
MS.gene043885.t1 AT1G09060 32.440 746 408 15 127 827 211 905 1.99e-120 387
MS.gene043885.t1 AT4G21430 30.013 743 431 12 129 830 222 916 4.58e-102 337

Find 187 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCCACCATGTAGATATTT+TGG 0.122618 5.3:-51349524 None:intergenic
GGATTTGTATATTCTTTAAA+TGG 0.213495 5.3:-51350531 None:intergenic
AGTACATGCGCTCATCATTA+TGG 0.215750 5.3:-51349270 None:intergenic
TCTTTACTGGGTACACAAAT+TGG 0.224530 5.3:+51350397 MS.gene043885:CDS
GATCTTCATCATGATGATTT+AGG 0.224559 5.3:+51349914 MS.gene043885:CDS
CTTCGTTGTGGGCAGCTTCT+AGG 0.236807 5.3:+51349461 MS.gene043885:CDS
ATTCCTTAGATGGTGCTCTT+TGG 0.245043 5.3:+51351465 MS.gene043885:CDS
TTCCTTAGATGGTGCTCTTT+GGG 0.262549 5.3:+51351466 MS.gene043885:CDS
AATGTGCACCAATTCTTTAC+TGG 0.265399 5.3:+51350384 MS.gene043885:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 5.3:-51349968 None:intergenic
AAACAAGAACAAGTCACTTT+TGG 0.285165 5.3:+51350057 MS.gene043885:CDS
TCACATTTAATCTCAGGTTT+TGG 0.298428 5.3:-51349656 None:intergenic
CTTTCTTCAAATAGTTTAGA+AGG 0.302686 5.3:-51350471 None:intergenic
TACCTCCAATTTGTCCTCTA+TGG 0.305460 5.3:-51354385 None:intergenic
TCATATTGCTGAAGCAAAAC+TGG 0.320106 5.3:+51351316 MS.gene043885:CDS
GCTCTTGTTTGAAGCCTGTT+AGG 0.324611 5.3:+51349804 MS.gene043885:CDS
TCTATGGACCTGTGATTTAC+TGG 0.329135 5.3:-51354369 None:intergenic
TTATTATCTCTGTTCCTAAC+AGG 0.330429 5.3:-51349818 None:intergenic
GGAGCTACATGGTGATATTC+AGG 0.343116 5.3:+51351400 MS.gene043885:CDS
ATGCCCGATGGTTAGATAAA+AGG 0.344516 5.3:-51352880 None:intergenic
AGGCCAATTCTGTTTATCCT+TGG 0.348155 5.3:-51350421 None:intergenic
AGCATCAGGTGCTTTGTATC+TGG 0.350049 5.3:-51349561 None:intergenic
ATCCCAAAGAGCACCATCTA+AGG 0.353655 5.3:-51351468 None:intergenic
GGCCAATTCTGTTTATCCTT+GGG 0.360073 5.3:-51350420 None:intergenic
ATTCGTACATGGTCAAAATC+TGG 0.362867 5.3:+51349599 MS.gene043885:CDS
GTTGATGGGGATTCCTTAGA+TGG 0.367964 5.3:+51351455 MS.gene043885:CDS
CTTGGCTTCTATCTCCTTCT+CGG 0.376051 5.3:-51348928 None:intergenic
AGAAGCATTTCAGAGAGTTC+AGG 0.389778 5.3:+51351534 MS.gene043885:CDS
GTACATGCGCTCATCATTAT+GGG 0.392626 5.3:-51349269 None:intergenic
AAGTATTGAAATTGAAAGAT+TGG 0.403818 5.3:+51350445 MS.gene043885:CDS
AGAGGACGTACCTGCATTGC+AGG 0.406514 5.3:+51351502 MS.gene043885:CDS
CAGCTCTTTGCAAAGAATCC+AGG 0.410316 5.3:+51349861 MS.gene043885:CDS
TCTTCCACATTTAATACGAT+TGG 0.410475 5.3:+51348890 MS.gene043885:CDS
TCTTTCTAGAATAGACATAA+TGG 0.411099 5.3:-51347889 None:intergenic
TTCAATGGCACTGGAGCAAA+GGG 0.413215 5.3:+51349942 MS.gene043885:CDS
TCAGGCTGGGCACCAGCATC+AGG 0.415198 5.3:-51349575 None:intergenic
GAAAATAGTTTGTTGGTTGA+TGG 0.415576 5.3:+51351440 MS.gene043885:CDS
TTGTGTACCCAGTAAAGAAT+TGG 0.417175 5.3:-51350392 None:intergenic
AATGTACTTGAATGTACATC+TGG 0.421952 5.3:+51349983 MS.gene043885:CDS
TATGGATTTGCTCAGGAGCT+TGG 0.422137 5.3:+51350636 MS.gene043885:CDS
TTACCTTCAGATTTCGGACT+TGG 0.425214 5.3:-51353096 None:intergenic
AAACCACCGGTAAACGTAAC+CGG 0.429322 5.3:+51347975 MS.gene043885:CDS
CCGTGATCACATTTAATCTC+AGG 0.429409 5.3:-51349662 None:intergenic
AAAACAGACTCCTCACAATA+AGG 0.433520 5.3:+51348174 MS.gene043885:CDS
AACAAACGCGGGAGCGGTGG+CGG 0.439355 5.3:+51347919 MS.gene043885:CDS
AGGCACTTGATTGCTTAGAT+TGG 0.440544 5.3:+51350086 MS.gene043885:CDS
TTCAGATATTCCTGCAATGC+AGG 0.441628 5.3:-51351512 None:intergenic
AAGAGCTTTGAGCATATACT+TGG 0.441941 5.3:-51348868 None:intergenic
GGCCCAAGGATAAACAGAAT+TGG 0.443826 5.3:+51350418 MS.gene043885:CDS
AAGATAAACTCAAAATCAAA+TGG 0.449448 5.3:-51349494 None:intergenic
TTTGACCATGTACGAATCTC+AGG 0.451399 5.3:-51349593 None:intergenic
AATGTACATCTGGTTTAAGC+TGG 0.451500 5.3:+51349993 MS.gene043885:CDS
GACAAAATCAAGTGCAACCT+TGG 0.456526 5.3:-51354292 None:intergenic
TCTGGCTTTAGAACTTCATC+AGG 0.461474 5.3:-51350588 None:intergenic
GTCAAAGAAATGACAGTGGC+AGG 0.462164 5.3:+51348464 MS.gene043885:CDS
TTCTTCTTGTCTACCAAGAC+GGG 0.465663 5.3:-51348073 None:intergenic
CAGGTCCATAGAGGACAAAT+TGG 0.466017 5.3:+51354380 MS.gene043885:CDS
TTCAGATTTCGGACTTGGTG+GGG 0.467992 5.3:-51353091 None:intergenic
ATTTGCTCAGGAGCTTGGAC+GGG 0.473572 5.3:+51350641 MS.gene043885:CDS
CCTTCAGATTTCGGACTTGG+TGG 0.476898 5.3:-51353093 None:intergenic
AAACGCGGGAGCGGTGGCGG+AGG 0.477252 5.3:+51347922 MS.gene043885:CDS
GATTTGCTCAGGAGCTTGGA+CGG 0.479287 5.3:+51350640 MS.gene043885:CDS
ACGATTGGATCAAGAACAAA+TGG 0.480998 5.3:+51348905 MS.gene043885:CDS
ACTACAGTCAATTAAAGAAA+AGG 0.482579 5.3:+51348809 MS.gene043885:intron
AAAATAGTTTGTTGGTTGAT+GGG 0.484733 5.3:+51351441 MS.gene043885:CDS
TATTGCTTATGGATTTGCTC+AGG 0.486399 5.3:+51350629 MS.gene043885:CDS
TGCGGAATTCCTCTGTCAAA+CGG 0.487229 5.3:-51354341 None:intergenic
GTTGCCGTGAGCTTCGTTGT+GGG 0.489346 5.3:+51349450 MS.gene043885:CDS
ACTAACTTTACCTCCTCTAC+AGG 0.489467 5.3:-51351572 None:intergenic
TGTGATTTACTGGAAGTTTG+CGG 0.491065 5.3:-51354359 None:intergenic
ATGTGCACCAATTCTTTACT+GGG 0.492244 5.3:+51350385 MS.gene043885:CDS
CTTCAGATTTCGGACTTGGT+GGG 0.495361 5.3:-51353092 None:intergenic
CACTGTTGAAAATAGTTTGT+TGG 0.496892 5.3:+51351433 MS.gene043885:CDS
TTTCTTCTTGTCTACCAAGA+CGG 0.504344 5.3:-51348074 None:intergenic
ACTAACTCAGAGATGCAATC+TGG 0.506716 5.3:-51349713 None:intergenic
GTTGTGGGCAGCTTCTAGGT+GGG 0.507656 5.3:+51349465 MS.gene043885:CDS
GGAAACCAACGATCATCATC+AGG 0.509624 5.3:+51348836 MS.gene043885:CDS
TGTATGTAGGTATTGAGCCT+TGG 0.510562 5.3:+51353020 MS.gene043885:intron
TGTATATTCTTTAAATGGCA+AGG 0.512473 5.3:-51350526 None:intergenic
GATAAGAAGAACAGCAGTAA+AGG 0.512786 5.3:+51348048 MS.gene043885:CDS
CGTTGTGGGCAGCTTCTAGG+TGG 0.513332 5.3:+51349464 MS.gene043885:CDS
TTTCAATGGCACTGGAGCAA+AGG 0.515097 5.3:+51349941 MS.gene043885:CDS
AGCAGTAAAGGCTCCCGTCT+TGG 0.517014 5.3:+51348060 MS.gene043885:CDS
GCTCCCTCTTGTCTTGCTCA+AGG 0.519343 5.3:-51351382 None:intergenic
ACCATGTACGAATCTCAGGC+TGG 0.519873 5.3:-51349589 None:intergenic
AACAACAACACATTCACGAC+GGG 0.521041 5.3:+51347848 MS.gene043885:CDS
TGTTGCCGTGAGCTTCGTTG+TGG 0.522924 5.3:+51349449 MS.gene043885:CDS
CTGAAGCAAAACTGGATTGT+GGG 0.525366 5.3:+51351324 MS.gene043885:CDS
TCTACATGGTGGAGTTGCAC+AGG 0.525763 5.3:+51349532 MS.gene043885:CDS
GGAAGAAGAAGCAGCTTCAT+CGG 0.527580 5.3:-51348002 None:intergenic
TCTTTCAATTTCAATACTTG+AGG 0.529125 5.3:-51350441 None:intergenic
TTTGTCTCTCCTGAAAATGT+TGG 0.534278 5.3:+51354309 MS.gene043885:CDS
TTCGTACATGGTCAAAATCT+GGG 0.534280 5.3:+51349600 MS.gene043885:CDS
TCAGATTTCGGACTTGGTGG+GGG 0.534353 5.3:-51353090 None:intergenic
TCAAGAACAAATGGCCGAGA+AGG 0.538911 5.3:+51348914 MS.gene043885:CDS
TGATCCAATCGTATTAAATG+TGG 0.539950 5.3:-51348894 None:intergenic
GAGATAGAAGCCAAGTTACA+AGG 0.542112 5.3:+51348936 MS.gene043885:CDS
AATGCACGCCACATGACAAA+CGG 0.544435 5.3:-51350019 None:intergenic
GTCAATTAAAGAAAAGGAGG+AGG 0.545970 5.3:+51348815 MS.gene043885:intron
TTTGCTCAGGAGCTTGGACG+GGG 0.547453 5.3:+51350642 MS.gene043885:CDS
ATAATGATGAGCGCATGTAC+TGG 0.548032 5.3:+51349272 MS.gene043885:CDS
AAGGTATCCTCACTTGAAAG+AGG 0.549713 5.3:+51348620 MS.gene043885:intron
AGCTCTTTGCAAAGAATCCA+GGG 0.549815 5.3:+51349862 MS.gene043885:CDS
TGTCCATTGCTCTCCTGTAG+AGG 0.552261 5.3:+51351559 MS.gene043885:CDS
TTAGATAAAAGGTCTGATCA+TGG 0.552811 5.3:-51352869 None:intergenic
TCAGATGCACCAAATGCAAA+CGG 0.554343 5.3:+51348491 MS.gene043885:CDS
TAAAAGGTCTGATCATGGAT+GGG 0.555220 5.3:-51352864 None:intergenic
ATAAAAGGTCTGATCATGGA+TGG 0.557609 5.3:-51352865 None:intergenic
CTCGACCTGATGATGATCGT+TGG 0.562673 5.3:-51348841 None:intergenic
AGGTTGAGAGCACCTTTGAA+AGG 0.563537 5.3:-51350552 None:intergenic
ACTCAGAACAAACGCGGGAG+CGG 0.564215 5.3:+51347913 MS.gene043885:CDS
AAGAAGAAGAATCTTGGTGA+TGG 0.565270 5.3:+51348100 MS.gene043885:CDS
CGAGGCCCAAAATATCTACA+TGG 0.566761 5.3:+51349518 MS.gene043885:CDS
ATCATCATGATGAAGATCTA+CGG 0.566812 5.3:-51349909 None:intergenic
GCTGGGAGCCGTTTGTCATG+TGG 0.567136 5.3:+51350011 MS.gene043885:CDS
AGCATTCTGTGCAGCTTCTG+TGG 0.567239 5.3:-51349406 None:intergenic
GAATATACAAATCCTTTCAA+AGG 0.567529 5.3:+51350540 MS.gene043885:CDS
GCACCTTGAGCAAGACAAGA+GGG 0.570458 5.3:+51351379 MS.gene043885:CDS
AAGTTTACCGCGTCATTGTG+CGG 0.570831 5.3:+51350491 MS.gene043885:CDS
TGATGAAGATCTACGGCCCT+AGG 0.571901 5.3:-51349902 None:intergenic
ATGTACATCTGGTTTAAGCT+GGG 0.572127 5.3:+51349994 MS.gene043885:CDS
AATTGTTCCTCTTTCAAGTG+AGG 0.572865 5.3:-51348627 None:intergenic
CTAGAATAGACATAATGGAT+AGG 0.573963 5.3:-51347884 None:intergenic
AGATACAAAGCACCTGATGC+TGG 0.574516 5.3:+51349563 MS.gene043885:CDS
AGCTTCTGAACAAAAGTCCA+AGG 0.575249 5.3:-51353037 None:intergenic
TTTCTTTCGAGAAATTGTGT+TGG 0.575306 5.3:-51348140 None:intergenic
CCTGAGATTAAATGTGATCA+CGG 0.577057 5.3:+51349662 MS.gene043885:CDS
CATCAGGCAGCTTCACAGCA+AGG 0.577705 5.3:-51350572 None:intergenic
ATAATAAACACAATAACGCA+AGG 0.582707 5.3:+51349834 MS.gene043885:CDS
AAATAGTTTGTTGGTTGATG+GGG 0.585934 5.3:+51351442 MS.gene043885:CDS
GAACAACAACAACAAACCAC+CGG 0.586106 5.3:+51347962 MS.gene043885:CDS
GTCCATAGAGGACAAATTGG+AGG 0.587047 5.3:+51354383 MS.gene043885:CDS
AGTAAATCACAGGTCCATAG+AGG 0.591019 5.3:+51354371 MS.gene043885:CDS
CAGAACAAACGCGGGAGCGG+TGG 0.591548 5.3:+51347916 MS.gene043885:CDS
CAAGACAAGAGGGAGCTACA+TGG 0.591607 5.3:+51351389 MS.gene043885:CDS
GCTGCCCACAACGAAGCTCA+CGG 0.592775 5.3:-51349454 None:intergenic
GAATAGACATAATGGATAGG+AGG 0.597179 5.3:-51347881 None:intergenic
TTGCAGTTACCACAGCAGAC+AGG 0.598583 5.3:-51348663 None:intergenic
GCAAACTTCCAGTAAATCAC+AGG 0.600882 5.3:+51354361 MS.gene043885:CDS
TTGTGGGCAGCTTCTAGGTG+GGG 0.602777 5.3:+51349466 MS.gene043885:CDS
AAAAGGTCTGATCATGGATG+GGG 0.603357 5.3:-51352863 None:intergenic
TCTTCAAATAGTTTAGAAGG+AGG 0.604177 5.3:-51350468 None:intergenic
AGAATTTAAGCATACTGCAT+CGG 0.606233 5.3:-51350697 None:intergenic
ACAGTCAATTAAAGAAAAGG+AGG 0.606305 5.3:+51348812 MS.gene043885:intron
CCCAGCCTGAGATTCGTACA+TGG 0.606376 5.3:+51349588 MS.gene043885:CDS
AAGAATCTTGGTGATGGTGA+TGG 0.607702 5.3:+51348106 MS.gene043885:CDS
CAATAAACAGTGGATTGAAG+AGG 0.607751 5.3:+51348420 MS.gene043885:intron
AACGAAGCTCACGGCAACAG+AGG 0.614200 5.3:-51349445 None:intergenic
GCTGAAGCAAAACTGGATTG+TGG 0.616163 5.3:+51351323 MS.gene043885:CDS
TTGACTCTTTCGAACAGCAA+CGG 0.617245 5.3:-51348023 None:intergenic
GAGGCATGTCCTGTCTGCTG+TGG 0.617423 5.3:+51348654 MS.gene043885:CDS
AGCACCTTGAGCAAGACAAG+AGG 0.618569 5.3:+51351378 MS.gene043885:CDS
TGAAGTTCTAAAGCCAGACA+TGG 0.620406 5.3:+51350593 MS.gene043885:CDS
AATGAAACTTCAAGATGCAG+AGG 0.623550 5.3:+51349763 MS.gene043885:CDS
CCATGTACGAATCTCAGGCT+GGG 0.624774 5.3:-51349588 None:intergenic
CTACATGGTGATATTCAGGA+TGG 0.633510 5.3:+51351404 MS.gene043885:CDS
CCATTGCTCTCCTGTAGAGG+AGG 0.634485 5.3:+51351562 MS.gene043885:CDS
CCACCAAGTCCGAAATCTGA+AGG 0.638088 5.3:+51353093 MS.gene043885:CDS
TCAAAGAAATGACAGTGGCA+GGG 0.638575 5.3:+51348465 MS.gene043885:CDS
TTGCTGTTCGAAAGAGTCAA+CGG 0.641060 5.3:+51348026 MS.gene043885:CDS
GAAAGAGGAACAATTTGCTG+AGG 0.645723 5.3:+51348635 MS.gene043885:CDS
CACGGCAACAGAGGAGACAA+AGG 0.649807 5.3:-51349436 None:intergenic
CCTCCTCTACAGGAGAGCAA+TGG 0.652097 5.3:-51351562 None:intergenic
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 5.3:+51354332 MS.gene043885:CDS
TTTCCGGTTACGTTTACCGG+TGG 0.665565 5.3:-51347978 None:intergenic
GAAGTTCTAAAGCCAGACAT+GGG 0.665837 5.3:+51350594 MS.gene043885:CDS
GGGTGGCATGTAGATAGTGA+TGG 0.666179 5.3:+51349620 MS.gene043885:CDS
CAACAACAACACATTCACGA+CGG 0.666770 5.3:+51347847 MS.gene043885:CDS
AACTCAGAGATGCAATCTGG+CGG 0.666994 5.3:-51349710 None:intergenic
TACATGCGCTCATCATTATG+GGG 0.667563 5.3:-51349268 None:intergenic
ACAATTTCTCGAAAGAAACC+CGG 0.668784 5.3:+51348145 MS.gene043885:CDS
TGGGTACACAAATTGGCCCA+AGG 0.673718 5.3:+51350404 MS.gene043885:CDS
TAATAAACACAATAACGCAA+GGG 0.674880 5.3:+51349835 MS.gene043885:CDS
ATAAACTCCGCACAATGACG+CGG 0.675233 5.3:-51350498 None:intergenic
GACGGGGTGATTCAGTGACT+AGG 0.676943 5.3:+51350658 MS.gene043885:CDS
CGTATATGCTATGATTGATG+TGG 0.680480 5.3:+51354813 MS.gene043885:CDS
GGCCCAAAATATCTACATGG+TGG 0.682965 5.3:+51349521 MS.gene043885:CDS
AAGAGACTCAGAACAAACGC+GGG 0.691625 5.3:+51347908 MS.gene043885:CDS
TGATTGCTTAGATTGGTGCG+AGG 0.693830 5.3:+51350093 MS.gene043885:CDS
GATGTTGTGACCTTATTGTG+AGG 0.707489 5.3:-51348184 None:intergenic
AAAGAGACTCAGAACAAACG+CGG 0.708789 5.3:+51347907 MS.gene043885:CDS
CTGCATCGGACATATCACAA+TGG 0.718824 5.3:-51350683 None:intergenic
CAATGTCAAAGAAATGACAG+TGG 0.718966 5.3:+51348460 MS.gene043885:CDS
GCTCTTTGCAAAGAATCCAG+GGG 0.726479 5.3:+51349863 MS.gene043885:CDS
ACAACAACACATTCACGACG+GGG 0.745848 5.3:+51347849 MS.gene043885:CDS
GTACATGGTCAAAATCTGGG+TGG 0.747902 5.3:+51349603 MS.gene043885:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 5.3:+51349943 MS.gene043885:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.3 gene 51347821 51354867 51347821 ID=MS.gene043885
chr5.3 mRNA 51347821 51354867 51347821 ID=MS.gene043885.t1;Parent=MS.gene043885
chr5.3 exon 51347821 51348195 51347821 ID=MS.gene043885.t1.exon1;Parent=MS.gene043885.t1
chr5.3 CDS 51347821 51348195 51347821 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51348430 51348545 51348430 ID=MS.gene043885.t1.exon2;Parent=MS.gene043885.t1
chr5.3 CDS 51348430 51348545 51348430 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51348623 51348722 51348623 ID=MS.gene043885.t1.exon3;Parent=MS.gene043885.t1
chr5.3 CDS 51348623 51348722 51348623 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51348816 51348957 51348816 ID=MS.gene043885.t1.exon4;Parent=MS.gene043885.t1
chr5.3 CDS 51348816 51348957 51348816 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51349230 51349293 51349230 ID=MS.gene043885.t1.exon5;Parent=MS.gene043885.t1
chr5.3 CDS 51349230 51349293 51349230 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51349376 51350114 51349376 ID=MS.gene043885.t1.exon6;Parent=MS.gene043885.t1
chr5.3 CDS 51349376 51350114 51349376 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51350375 51350704 51350375 ID=MS.gene043885.t1.exon7;Parent=MS.gene043885.t1
chr5.3 CDS 51350375 51350704 51350375 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51351302 51351583 51351302 ID=MS.gene043885.t1.exon8;Parent=MS.gene043885.t1
chr5.3 CDS 51351302 51351583 51351302 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51352855 51352927 51352855 ID=MS.gene043885.t1.exon9;Parent=MS.gene043885.t1
chr5.3 CDS 51352855 51352927 51352855 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51353029 51353114 51353029 ID=MS.gene043885.t1.exon10;Parent=MS.gene043885.t1
chr5.3 CDS 51353029 51353114 51353029 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51354285 51354404 51354285 ID=MS.gene043885.t1.exon11;Parent=MS.gene043885.t1
chr5.3 CDS 51354285 51354404 51354285 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
chr5.3 exon 51354799 51354867 51354799 ID=MS.gene043885.t1.exon12;Parent=MS.gene043885.t1
chr5.3 CDS 51354799 51354867 51354799 ID=cds.MS.gene043885.t1;Parent=MS.gene043885.t1
Gene Sequence

>MS.gene043885

ATGGCGTTTGCGAGGGAACAAGAAGACAACAACAACACATTCACGACGGGGATTAACAAGCCTCCTATCCATTATGTCTATTCTAGAAAGAGACTCAGAACAAACGCGGGAGCGGTGGCGGAGGAGTTAGCTAAGAACGAAGAACAACAACAACAAACCACCGGTAAACGTAACCGGAAAACCGATGAAGCTGCTTCTTCTTCCGTTGCTGTTCGAAAGAGTCAACGGATAAGAAGAACAGCAGTAAAGGCTCCCGTCTTGGTAGACAAGAAGAAAAAGAAGAAGAAGAATCTTGGTGATGGTGATGGTGCTGAGAAAACCAACACAATTTCTCGAAAGAAACCCGGAAAAAGAAAACAGACTCCTCACAATAAGGTCACAACATCTTCTTCTAGTTATTATTATTATTATTATTATTATGCACTGTGGTTTTGTAATAACCTATTAATCTCATACATAATTTGAGAAAGTAATATTTCAATTTTTATTTTATTTCGAGAAAATGAAGTAATTCGATGTAATTGTATGTGTTGGTGTCGGACATTGATGCGTGTCTGACACCGGACACACCTAATCTGATGAGTGACTAACTAACTATTCAATAAACAGTGGATTGAAGAGGAATCGTTAATGTGTCATCAATGTCAAAGAAATGACAGTGGCAGGGTTGTCAGATGCACCAAATGCAAACGGAAAAGATATTGTGTATGCTGTATAAAAAAATGGTATGTTTCTATGTTTCTGCAACTTGATTCATTCACCAATGTAATGTAATCTAATGTATATCGTTTTTGTTGCTAAGGTATCCTCACTTGAAAGAGGAACAATTTGCTGAGGCATGTCCTGTCTGCTGTGGTAACTGCAATTGCAAAGCTTGTTTGAAGTCACGAAAACTTATCGATGTATGTACTAATCTAAACTTAACTTCGACCTCTGCTATCTACATTATATCTATTCATTTATTAACATTTTAAATTGTCTTTTATTCACTACAGTCAATTAAAGAAAAGGAGGAGGAAACCAACGATCATCATCAGGTCGAGCGCTCCAAGTATATGCTCAAAGCTCTTCTTCCACATTTAATACGATTGGATCAAGAACAAATGGCCGAGAAGGAGATAGAAGCCAAGTTACAAGGTATTTTTTCCTCTCTTCTTGCTTTTTTGTCTATCCTCTTATTAATGTCACTTGTTTTTTCCATGGTTCACATTTTATATATACCTTTCTTTCTCTTCGATAACTTACATACTAATTCATATCCTGCTTTACACTATTTTTCCTGCATGTGCTTTTTGTTTATGTGTTACGTTTGTATTTCATCTCATGTGTCGGTCTGTCCGTGATTGATCATTTTTTAGTTTCATATTATTTTCCATTTTTTTAATTGGATTTTGGTATCAACGTTGCAGGTCTCTCACTCTCTGAGCTAAAAATTAAAAAATCAAATCCCCATAATGATGAGCGCATGTACTGGTAGGGACTTATTTGGTTTATAAATTGGATTTTTAGTTAGTTATTCCAATGCTATATTGTTAATGACTTATTACTTTTGTAGTGACAACTGCAAAACATCAATATTTGACTACCACAGAAGCTGCACAGAATGCTCTTTTGACCTTTGTCTCCTCTGTTGCCGTGAGCTTCGTTGTGGGCAGCTTCTAGGTGGGGCAGATCCATTTGATTTTGAGTTTATCTTCCGAGGCCCAAAATATCTACATGGTGGAGTTGCACAGGAAGTTACCAGATACAAAGCACCTGATGCTGGTGCCCAGCCTGAGATTCGTACATGGTCAAAATCTGGGTGGCATGTAGATAGTGATGGCAATATTCCCTGTCCAAAACCTGAGATTAAATGTGATCACGGTTACCTTGAACTGAAAAGCGTATTTCCGCCAGATTGCATCTCTGAGTTAGTATGTAAAGCAAAAGAAATTGCAGATTCAATGAAACTTCAAGATGCAGAGGTGACTCTTGATAATAGCTGCTCTTGTTTGAAGCCTGTTAGGAACAGAGATAATAAACACAATAACGCAAGGGAAGCAGCTCTTTGCAAAGAATCCAGGGGCAACTTTTTGTACTGTCCTAGGGCCGTAGATCTTCATCATGATGATTTAGGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAGCCGTTTGTCATGTGGCGTGCATTTCGTCAGATAAACAAAAACAAGAACAAGTCACTTTTGGATGTTAAGGCACTTGATTGCTTAGATTGGTGCGAGGTTTGTTCAATTTCTCAATCTTGAACATAAATTTTGTTTGAGTTCGTGATCTTTGAGCTTCTCATGCGGTTTCTTGCACTCTAGTGTCCCTTGCATCCCCTGATTTCTGTTTTTCTGTTGGTATTTTTCTCTGTTTCTTATGCATACTCACCCCAGTTGTGTTCATTTTCTTTCATTTCTGCATTTTTTTTACTATACTTGACATGCCTATAAGTTGCTTGTAACTTCTAAATTTTATTTTAGTTTTTGATTCTATGCAGATAGATATTAATGTGCACCAATTCTTTACTGGGTACACAAATTGGCCCAAGGATAAACAGAATTGGCCTCAAGTATTGAAATTGAAAGATTGGCCTCCTTCTAAACTATTTGAAGAAAGTTTACCGCGTCATTGTGCGGAGTTTATATCTTCCTTGCCATTTAAAGAATATACAAATCCTTTCAAAGGTGCTCTCAACCTTGCTGTGAAGCTGCCTGATGAAGTTCTAAAGCCAGACATGGGACCAAAAACATATATTGCTTATGGATTTGCTCAGGAGCTTGGACGGGGTGATTCAGTGACTAGGCTCCATTGTGATATGTCCGATGCAGTATGCTTAAATTCTCTGAGTTTTTCCTGGACTGTTTTTTCTAGAATGTCGTGTGTATTTTTTTTTTATGTTTATGTTGTTATTTATTATTTGAGTATCATATTAAAAGGGGCATAATTTGTAGGATGTATACGATGCCTTTGTAGGCCATTGCATTAAGAGCTGATAGTTGTGTAGTTTCAAGTACATCATATCTTAAGTTTTTGGTCATTGCTCTGTCTTTGAAACACTCATTTCATTATATATTTGATTTGAAATGCTATTTACCTTGGTATTTTGGTAATTGAAAATTGGATAGTATTATTTTTCTGATTAGTTGCATCATTAGATAAGGTATTTTTTGGTCTTGAAATATGAAAGATGGTTTTGGATTAGAATGTTTTACTCGATGTCTACTTCCATCAGATGCATTGTCTTTCCGGCCGGATTAATTATTTGTTTGTGCTTTATATGTTTTGCTTTGCGTTTGAGTTCTTACTTTTGTTTGTGCTTTATATGTTTTACTTTGTGTCTTCAGTTCTTTACTCTATTATGAGGAAAATTAAAGTTTTTTTTTTTGTTTTTTTTGTTTTTGTTTTTGTTTTTTTTGGCTTTTAGGTAAATGTGTTGACTCATATTGCTGAAGCAAAACTGGATTGTGGGAGTATTGACGCAATTAAGAAATTGAAACAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATATTCAGGATGGAGAGACCACTGTTGAAAATAGTTTGTTGGTTGATGGGGATTCCTTAGATGGTGCTCTTTGGGATATTTTTCGGAGAGAGGACGTACCTGCATTGCAGGAATATCTGAAGAAGCATTTCAGAGAGTTCAGGCATGTCCATTGCTCTCCTGTAGAGGAGGTAAAGTTAGTTGTTATAATTTTCTATCATCTATTTGGATACCCCCTGTTAACTGGAGTCATTAATGAAACTTAGTTGCTACCGATTAGTTTTGCTGCTGTGAACATGCTCTATAATTACCCTTTTATTTTTCACATTTTGCTGCCACGGACATGATAAAGTTGACACTATCTCATTGCTTTTCCCTATTTGACTTAATTCTTAGCTTATGCTTTATTTTATTTTATTTTATTTTTCTATGTTGTTATTGTTGGTTTGCTTATGTACCTTGTAATATGTTCTATGGGGCCTTCATAGTTTAGTCTCACCTAGCTTTGAAGGATGTAGCACACTCTGTCGTAGTTACAACTGTCTCTAACAATTTAACTCCCTATAAATTCCTATCAGTAATTTAAGTTACATCAAGTAGTTTAGTTTTCAAACAAAAGAAATAAAAAATGCCCATAATGAAGGTATCCTGCAGTGCTGGTCTGGGTTTGTCTAGGCATCGCATTGCCTATTGCCTGCGGCGTTGATTCTTTCTACCTTGCCCACTCTTTTTGTAGAGACCTTGTGCCTCTCTCTCTCTCTCTCACTCTCTCTCTCTCTCTCTCTGTTTTTTTCCTTCTGTCTACCCAAATCTCTCTTTTTATAGGGTTATGTTTGTTATACTACTGTACATTGTAATATGTTGTATTGGTCCTTGGTTGTTCAGTCCTGCAACACATTGGTAAGTTCTGCCTAGCTTAATAGGATCCAGCACACTTTGTTGGGGGAAACTGTCCATAACAGATTGCACTTCCCTATAAATCATTTTCGTACTTTCTGTTTTGCTAATAAGCTACATCAACAGAAAGTTATCTCAATTATCTGTTTACATTTTCCTTCATTATCTCTTGTTTTCTCAGCTAGTGATTACTTACATCTGTTAGGTTCAGATTTTTTTTCTTTTTTTTTTATTATGAAATTTACATGCCTCGAATGATCAAGCTGGTAATAAATAATATTTGTGAGTAGTTAGTTTTGAATACGGACCATCATGAGTATGGAATTTTGTTGATGCGAATAGCGGTATTGAAACATATGATATGAAGGGAAATGCATTATGGATGTAATTTCCAGTCCCTTAGGTCACCGATTCAGTAGATGTTCTTTCATTTCATTTTACTGTTTCAATTTCAAAGTTCACTTGGCAGTAGACGGGGCTTTTTATGTAGATGACTTGGCAGTCTGATGACGGTTCATTCATTATGTATGTATCTTTTTGAATGACATCTCCTTTGGCATTATAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCATCGGGCATAAGAAAAAGCTGAAGGAAGAGTATGGTTAGTAGTGCTTATATTTTACTTATTTTGTTTGATTTGTGGAGTGTAGATCAATAGGGTTGTACCATTTTGTTAACAATTCAACACTGACTTGTATGTAGGTATTGAGCCTTGGACTTTTGTTCAGAAGCTTGGAGATGCAGTTTTCATTCCAGCTGGTTGCCCCCACCAAGTCCGAAATCTGAAGGTAAAACTTGCATACATGGTGGATTTGTTTTATAATGAAGGCATACATTTTCCTTGTTTGCTAAGAAGTGTAGATGTTGATGTTATGAGTTTTTCAAATTTTTTTTTTGAGTAACGTTATGAGTTAGAAGTAGAGAAAAAATAAATATAAATATGGTTAAAGATGATTGATAATGACTTGATGACCAACTATTATGTTGCCAAGTTCTTGAAAGTTTGAGAATGTCCTAGTAGTTGTATACTGGTATTGTATTCAAAATCCTTGTTGAATTAGTGCTAAATAATTGTCATTTGACAATAATGTGATGTTAACTTGATACAAAAGACTAAATGCTAATTCCTAGGTAGAACATTATTACAACAAGGTCCTAGGTAAAAAATAACTTCCATATTCTGTTATACTTCTTATTATAGAACACTATAATAGGAGATTTCTTAGTTAGTTGGTTATATGCAGTTATGTTTTTATTATTATCTGTGTGTGTCGGTTATGTAAAGTGGGAAAGACATGAAAGAATTGGTTATATGGAAGTTAATTAAGTGGTTATTGGGACTCAAAATCATGGAGAACCAGCATAACCCAGCCCTGTTAAGTGTGTTCCTGTTCTGTCTTGTCTTGTAACAATCTCTGTGTTAATAATGTATATGTGTTTATATGAATCCCTTCTCCTAAGGTTAGATCAATAATATTCTATTGTATTTCATAAATAATTATTTATACTTGTACCAGTTTCTATCATATTCCTATTTAAACATTCCTAATGGGGGAGGGGTTTGGGATTTGGTTCCTATGGATGTGGGCATAGAATTTAGATTGAGCCTAATCAAATCACAAAATTAGCTTATAAGTGAGAGATGCTTCCCTCATAAATACTTCAAGCCATATCATCTAGACGTCTAGATCCCTACTCGAGTAGGGTGACATCGTAGCCAAATGGATAAAGCATAGCCTCTGATATCTTAGTATTTAGCTTGGTTCTAACTCATACCAATGAGTCACTGAAGTTAATATTGAGTGCATCTAAGTTCTTTTTGAAGACTGCATTTTTAATTTTTTTTTATACATATCAGTGTGGAGATTTGAACCTACAATTTCTTACAGATTACCCAATCAATTACTCACTAGCTCACTTTAATGTTCTTGCTAATGATATGCATCATCATTTTTCAGTCATGCACCAAGGTTGCACTTGATTTTGTCTCTCCTGAAAATGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTATGCCAGACTATTATGGATTTGATTCTTCTGTTTATATGGAGCTTTCAGTTACACCTATTTTTGGCATCGTCATGTTGCAATTGTCCAAATCTGAACATTTGTTGGTTGAACTGTTTCAAGCATATTATTCACATTTGTTTGAATTGATCATTTTTTACCATCTAGAATCTAGTAAAGAGTATTTGTTATATATTTTATATTTTCTTATCTAGTAGTATTGTAGCTTTTATGTAAAGACAAGGTTCAAATATTTAACTAATGTTTGGCAGTTTTGAGTGTAAAGCTATTTCAACCAACTATGTTACTGGGTCATACCATCATGTCATATCAACAGATTATTTTTGGTATTTTCTGTATAGTTTGTTCCTTATTGCTGCTTCTTTTGTAACAGGTGAAGAAAATGATCGTATATGCTATGATTGATGTGGTCAAAAAGTTGGAGAAAGCAAGGAGCCAATGA
Protein sequence

>MS.gene043885.t1

MAFAREQEDNNNTFTTGINKPPIHYVYSRKRLRTNAGAVAEELAKNEEQQQQTTGKRNRKTDEAASSSVAVRKSQRIRRTAVKAPVLVDKKKKKKKNLGDGDGAEKTNTISRKKPGKRKQTPHNKWIEEESLMCHQCQRNDSGRVVRCTKCKRKRYCVCCIKKWYPHLKEEQFAEACPVCCGNCNCKACLKSRKLIDSIKEKEEETNDHHQVERSKYMLKALLPHLIRLDQEQMAEKEIEAKLQGLSLSELKIKKSNPHNDERMYCDNCKTSIFDYHRSCTECSFDLCLLCCRELRCGQLLGGADPFDFEFIFRGPKYLHGGVAQEVTRYKAPDAGAQPEIRTWSKSGWHVDSDGNIPCPKPEIKCDHGYLELKSVFPPDCISELVCKAKEIADSMKLQDAEVTLDNSCSCLKPVRNRDNKHNNAREAALCKESRGNFLYCPRAVDLHHDDLGHFQWHWSKGEPVIVSNVLECTSGLSWEPFVMWRAFRQINKNKNKSLLDVKALDCLDWCEIDINVHQFFTGYTNWPKDKQNWPQVLKLKDWPPSKLFEESLPRHCAEFISSLPFKEYTNPFKGALNLAVKLPDEVLKPDMGPKTYIAYGFAQELGRGDSVTRLHCDMSDAVNVLTHIAEAKLDCGSIDAIKKLKQKHLEQDKRELHGDIQDGETTVENSLLVDGDSLDGALWDIFRREDVPALQEYLKKHFREFRHVHCSPVEEVIHPIHDQTFYLTIGHKKKLKEEYGIEPWTFVQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSIEDKLEVKKMIVYAMIDVVKKLEKARSQ