AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044186


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044186.t1 MTR_3g080100 95.686 255 11 0 1 255 1 255 0.0 497
MS.gene044186.t1 MTR_3g080100 95.833 216 9 0 40 255 1 216 1.60e-152 424
MS.gene044186.t1 MTR_8g089895 37.987 308 132 9 1 255 1 302 6.45e-54 176
MS.gene044186.t1 MTR_3g086790 40.604 298 129 8 1 255 1 293 1.70e-50 167
MS.gene044186.t1 MTR_6g011610 46.465 198 91 4 42 238 63 246 3.26e-41 142
MS.gene044186.t1 MTR_6g011610 46.465 198 91 4 42 238 82 265 3.28e-41 142
MS.gene044186.t1 MTR_8g468210 57.724 123 51 1 46 168 64 185 1.42e-38 136
MS.gene044186.t1 MTR_5g039000 57.600 125 52 1 44 168 62 185 2.67e-38 135
MS.gene044186.t1 MTR_5g038280 60.000 110 44 0 47 156 75 184 6.87e-37 132
MS.gene044186.t1 MTR_6g007647 64.286 98 35 0 58 155 32 129 9.54e-35 125
MS.gene044186.t1 MTR_8g469430 60.577 104 41 0 59 162 89 192 1.14e-34 126
MS.gene044186.t1 MTR_6g007647 64.286 98 35 0 58 155 66 163 2.27e-34 125
MS.gene044186.t1 MTR_4g107650 61.905 105 40 0 58 162 94 198 4.82e-34 125
MS.gene044186.t1 MTR_7g010020 51.938 129 58 2 47 174 71 196 6.91e-33 121
MS.gene044186.t1 MTR_7g103340 47.761 134 68 2 52 185 56 187 5.22e-30 112
MS.gene044186.t1 MTR_1g061660 49.550 111 56 0 58 168 58 168 1.29e-25 100
MS.gene044186.t1 MTR_8g026960 51.163 86 42 0 66 151 41 126 4.42e-24 97.1
MS.gene044186.t1 MTR_3g092150 48.352 91 47 0 61 151 40 130 3.83e-23 94.7
MS.gene044186.t1 MTR_4g097600 47.748 111 49 2 43 153 113 214 1.45e-20 88.6
MS.gene044186.t1 MTR_4g100550 46.667 105 49 1 49 153 110 207 4.51e-20 87.0
MS.gene044186.t1 MTR_5g019680 44.915 118 53 1 51 168 45 150 4.87e-20 85.5
MS.gene044186.t1 MTR_5g019650 45.000 120 57 3 51 168 45 157 2.46e-19 84.0
MS.gene044186.t1 MTR_1g054285 48.113 106 46 2 48 153 145 241 2.43e-18 82.8
MS.gene044186.t1 MTR_7g093430 47.170 106 47 2 48 153 151 247 2.53e-18 82.8
MS.gene044186.t1 MTR_2g061030 42.222 135 58 3 19 153 173 287 2.65e-18 83.2
MS.gene044186.t1 MTR_4g126900 39.130 138 74 2 19 153 45 175 8.25e-18 79.7
MS.gene044186.t1 MTR_5g013010 42.857 133 56 3 21 152 112 225 1.80e-17 80.5
MS.gene044186.t1 MTR_5g014890 46.226 106 49 2 48 153 113 210 4.26e-17 79.0
MS.gene044186.t1 MTR_1g017090 31.667 180 81 5 59 226 130 279 2.69e-16 77.0
MS.gene044186.t1 MTR_8g006705 46.316 95 44 1 59 153 146 233 6.55e-16 76.3
MS.gene044186.t1 MTR_4g084750 44.211 95 46 1 59 153 65 152 1.89e-14 70.1
MS.gene044186.t1 MTR_3g103590 38.406 138 68 4 57 186 143 271 8.30e-14 70.1
MS.gene044186.t1 MTR_2g038625 44.211 95 46 1 59 153 82 169 3.63e-13 67.0
MS.gene044186.t1 MTR_2g038580 44.211 95 46 1 59 153 82 169 3.63e-13 67.0
MS.gene044186.t1 MTR_5g013010 43.956 91 35 2 21 110 112 187 2.74e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044186.t1 AT5G65310 53.571 168 65 4 1 155 1 168 4.24e-49 164
MS.gene044186.t1 AT5G65310 59.441 143 47 2 24 155 8 150 9.84e-49 162
MS.gene044186.t1 AT4G40060 35.065 308 123 9 1 255 1 284 1.73e-44 152
MS.gene044186.t1 AT2G22430 49.057 159 75 2 1 155 2 158 2.01e-42 147
MS.gene044186.t1 AT1G69780 60.163 123 48 1 46 168 72 193 2.30e-40 141
MS.gene044186.t1 AT5G15150 58.594 128 49 3 42 166 95 221 5.33e-39 138
MS.gene044186.t1 AT1G26960 44.000 200 97 5 41 235 53 242 1.01e-37 133
MS.gene044186.t1 AT3G01220 43.548 186 95 4 44 224 70 250 2.08e-35 128
MS.gene044186.t1 AT3G01470 60.000 100 40 0 56 155 65 164 3.96e-33 121
MS.gene044186.t1 AT3G61890 39.161 143 82 2 62 202 33 172 1.64e-27 106
MS.gene044186.t1 AT1G27045 54.545 99 45 0 57 155 66 164 5.79e-27 104
MS.gene044186.t1 AT1G27045 54.545 99 45 0 57 155 15 113 8.99e-27 102
MS.gene044186.t1 AT1G27045 54.545 99 45 0 57 155 23 121 1.04e-26 102
MS.gene044186.t1 AT1G27045 54.545 99 45 0 57 155 23 121 1.04e-26 102
MS.gene044186.t1 AT2G46680 50.538 93 46 0 60 152 33 125 2.73e-26 103
MS.gene044186.t1 AT1G27045 53.684 95 44 0 57 151 66 160 1.97e-25 100
MS.gene044186.t1 AT2G46680 48.958 96 48 1 60 154 33 128 6.32e-25 99.8
MS.gene044186.t1 AT2G18550 39.521 167 78 4 59 217 61 212 1.50e-22 92.8
MS.gene044186.t1 AT5G03790 50.000 92 46 0 59 150 77 168 1.19e-21 90.5
MS.gene044186.t1 AT4G36740 47.368 114 56 1 52 161 48 161 2.42e-21 89.4
MS.gene044186.t1 AT4G36740 47.368 114 56 1 52 161 49 162 2.76e-21 89.4
MS.gene044186.t1 AT5G03790 52.439 82 39 0 59 140 77 158 6.87e-21 87.4
MS.gene044186.t1 AT2G36610 45.545 101 47 1 58 150 70 170 1.03e-20 87.0
MS.gene044186.t1 AT5G66700 35.429 175 101 5 46 217 60 225 2.76e-20 86.7
MS.gene044186.t1 AT5G53980 42.268 97 56 0 59 155 11 107 4.22e-19 81.6
MS.gene044186.t1 AT2G01430 43.119 109 55 1 45 153 125 226 3.00e-18 82.0
MS.gene044186.t1 AT5G47370 42.424 132 63 2 21 152 101 219 4.35e-18 82.0
MS.gene044186.t1 AT4G17460 42.063 126 62 3 32 153 104 222 1.31e-17 80.5
MS.gene044186.t1 AT5G06710 33.333 171 103 4 20 187 152 314 1.44e-17 81.3
MS.gene044186.t1 AT4G37790 42.241 116 59 2 38 153 106 213 8.10e-17 78.2
MS.gene044186.t1 AT2G44910 46.316 95 44 1 59 153 163 250 6.11e-16 76.3
MS.gene044186.t1 AT2G22800 40.650 123 71 2 48 169 101 222 1.50e-15 74.7
MS.gene044186.t1 AT3G60390 42.593 108 58 1 45 152 148 251 3.12e-15 74.3
MS.gene044186.t1 AT4G16780 43.689 103 50 2 51 153 122 216 7.41e-15 72.8
MS.gene044186.t1 AT2G01430 43.529 85 44 1 45 125 125 209 1.09e-12 65.9
MS.gene044186.t1 AT1G70920 38.525 122 73 1 23 144 5 124 4.25e-12 63.5
MS.gene044186.t1 AT1G70920 38.525 122 73 1 23 144 35 154 4.38e-12 63.9
MS.gene044186.t1 AT5G06710 39.560 91 54 1 20 110 152 241 5.37e-12 64.7

Find 36 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAAGTTCAATGATGAATT+TGG 0.158147 3.4:-76471847 MS.gene044186:CDS
TCGTTCGTGCTCGACGATTT+TGG 0.160811 3.4:+76472245 None:intergenic
CTTTCTATACCTCTTGAAAT+TGG 0.202435 3.4:+76471951 None:intergenic
AACCTTAAGAACATCAAAAT+TGG 0.244715 3.4:+76472183 None:intergenic
TTGAAGTTAGCTGAAGAATT+AGG 0.251335 3.4:-76472298 MS.gene044186:CDS
AACCTAGACAAGTTTCAATT+TGG 0.262579 3.4:-76472270 MS.gene044186:CDS
TAATTCAAGTGGTGTGATTA+AGG 0.320954 3.4:-76471913 MS.gene044186:CDS
ATAATCTCTTTCCAATTGTT+TGG 0.328373 3.4:+76472219 None:intergenic
TTTAGAAAAGAGCTTTGAAT+TGG 0.355867 3.4:-76472347 MS.gene044186:CDS
GATTCTCCTATATGTTTCAA+TGG 0.381447 3.4:-76471876 MS.gene044186:CDS
GTTCAATAACTCAGAAACTA+TGG 0.387793 3.4:-76472660 MS.gene044186:CDS
TCAGAATCAATTTATGAGAA+TGG 0.408136 3.4:-76471784 MS.gene044186:CDS
CATACCTTTGGATTGGTACA+TGG 0.415046 3.4:+76472625 None:intergenic
ACCAAATTCATCATTGAACT+TGG 0.453246 3.4:+76471846 None:intergenic
ACTTATACATACCTTTGGAT+TGG 0.465929 3.4:+76472618 None:intergenic
CTCAGTTGGTATTACAAAGT+AGG 0.474989 3.4:+76471702 None:intergenic
AAAGAACCATTGAAACATAT+AGG 0.475600 3.4:+76471870 None:intergenic
CTTTGAATTGGACAACAAGC+TGG 0.480175 3.4:-76472335 MS.gene044186:CDS
TCAGTTGGTATTACAAAGTA+GGG 0.482035 3.4:+76471703 None:intergenic
ACGAACGAAAACCAAACAAT+TGG 0.484787 3.4:-76472230 MS.gene044186:CDS
GAATGAGACTCTTACTAGAA+AGG 0.510168 3.4:-76472137 MS.gene044186:intron
TGCAAATGCAGGTGTGGATG+TGG 0.525574 3.4:-76472506 MS.gene044186:intron
CAAGAAGATAGCTATGAAGA+TGG 0.532967 3.4:-76472421 MS.gene044186:CDS
TGAAGATGGTAGTTCAATGT+TGG 0.535009 3.4:-76472407 MS.gene044186:CDS
AACCAAATTGAAACTTGTCT+AGG 0.545141 3.4:+76472268 None:intergenic
ATTTCAAGAGGTATAGAAAG+AGG 0.548836 3.4:-76471948 MS.gene044186:CDS
GATCAGAGGAATGAGACAAA+AGG 0.552396 3.4:-76472484 MS.gene044186:CDS
CTTTCCATGTACCAATCCAA+AGG 0.553684 3.4:-76472629 MS.gene044186:intron
TTGGAGAAGAAAAGAAGACT+TGG 0.569876 3.4:-76472388 MS.gene044186:CDS
GTTCATTATTATGATTCAAG+AGG 0.577370 3.4:-76471825 MS.gene044186:CDS
GTAGAATGTCCAATTTCAAG+AGG 0.608484 3.4:-76471960 MS.gene044186:CDS
GAATCAATTTATGAGAATGG+AGG 0.629666 3.4:-76471781 MS.gene044186:CDS
TCAAGAGGTATAGAAAGAGG+AGG 0.641487 3.4:-76471945 MS.gene044186:CDS
CAGTTGGTATTACAAAGTAG+GGG 0.656897 3.4:+76471704 None:intergenic
GGTTCAGAAAGTAATTCAAG+TGG 0.665455 3.4:-76471924 MS.gene044186:CDS
CAGGTGTGGATGTGGATCAG+AGG 0.671722 3.4:-76472498 MS.gene044186:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATACACACATAAATTA+TGG + chr3.4:76471831-76471850 None:intergenic 15.0%
!! ACACATAAATTATGGTAAAA+AGG + chr3.4:76471823-76471842 None:intergenic 20.0%
!! CACATAAATTATGGTAAAAA+GGG + chr3.4:76471822-76471841 None:intergenic 20.0%
!! TAATTACTTATACATACCTT+TGG + chr3.4:76471771-76471790 None:intergenic 20.0%
!!! AATTTATGTGTGTATTTTTG+TGG - chr3.4:76471832-76471851 MS.gene044186:CDS 20.0%
! AAAGAACCATTGAAACATAT+AGG + chr3.4:76472514-76472533 None:intergenic 25.0%
! AACCTTAAGAACATCAAAAT+TGG + chr3.4:76472201-76472220 None:intergenic 25.0%
! ATAATCTCTTTCCAATTGTT+TGG + chr3.4:76472165-76472184 None:intergenic 25.0%
! GTTCATTATTATGATTCAAG+AGG - chr3.4:76472556-76472575 MS.gene044186:intron 25.0%
! TCAGAATCAATTTATGAGAA+TGG - chr3.4:76472597-76472616 MS.gene044186:intron 25.0%
!!! TTTAGAAAAGAGCTTTGAAT+TGG - chr3.4:76472034-76472053 MS.gene044186:intron 25.0%
AACCAAATTGAAACTTGTCT+AGG + chr3.4:76472116-76472135 None:intergenic 30.0%
AACCTAGACAAGTTTCAATT+TGG - chr3.4:76472111-76472130 MS.gene044186:intron 30.0%
ACCAAATTCATCATTGAACT+TGG + chr3.4:76472538-76472557 None:intergenic 30.0%
ACTTATACATACCTTTGGAT+TGG + chr3.4:76471766-76471785 None:intergenic 30.0%
CTTTCTATACCTCTTGAAAT+TGG + chr3.4:76472433-76472452 None:intergenic 30.0%
GAATCAATTTATGAGAATGG+AGG - chr3.4:76472600-76472619 MS.gene044186:intron 30.0%
GATTCTCCTATATGTTTCAA+TGG - chr3.4:76472505-76472524 MS.gene044186:intron 30.0%
GTTCAATAACTCAGAAACTA+TGG - chr3.4:76471721-76471740 MS.gene044186:CDS 30.0%
TAATTCAAGTGGTGTGATTA+AGG - chr3.4:76472468-76472487 MS.gene044186:CDS 30.0%
TTGAAGTTAGCTGAAGAATT+AGG - chr3.4:76472083-76472102 MS.gene044186:intron 30.0%
! ATTTCAAGAGGTATAGAAAG+AGG - chr3.4:76472433-76472452 MS.gene044186:CDS 30.0%
!! GTCCAATTTTGATGTTCTTA+AGG - chr3.4:76472196-76472215 MS.gene044186:CDS 30.0%
ACGAACGAAAACCAAACAAT+TGG - chr3.4:76472151-76472170 MS.gene044186:CDS 35.0%
CAAGAAGATAGCTATGAAGA+TGG - chr3.4:76471960-76471979 MS.gene044186:CDS 35.0%
GAATGAGACTCTTACTAGAA+AGG - chr3.4:76472244-76472263 MS.gene044186:CDS 35.0%
GCCAAGTTCAATGATGAATT+TGG - chr3.4:76472534-76472553 MS.gene044186:intron 35.0%
GGTTCAGAAAGTAATTCAAG+TGG - chr3.4:76472457-76472476 MS.gene044186:CDS 35.0%
GTAGAATGTCCAATTTCAAG+AGG - chr3.4:76472421-76472440 MS.gene044186:CDS 35.0%
GTTTCATTCTTTGCAAATGC+AGG - chr3.4:76471864-76471883 MS.gene044186:CDS 35.0%
TGAAGATGGTAGTTCAATGT+TGG - chr3.4:76471974-76471993 MS.gene044186:CDS 35.0%
TTGGAGAAGAAAAGAAGACT+TGG - chr3.4:76471993-76472012 MS.gene044186:CDS 35.0%
! GCTAACTTCAATTTTCTCTC+TGG + chr3.4:76472074-76472093 None:intergenic 35.0%
ATTCTTTGCAAATGCAGGTG+TGG - chr3.4:76471869-76471888 MS.gene044186:CDS 40.0%
CTTTCCATGTACCAATCCAA+AGG - chr3.4:76471752-76471771 MS.gene044186:CDS 40.0%
CTTTGAATTGGACAACAAGC+TGG - chr3.4:76472046-76472065 MS.gene044186:intron 40.0%
GATCAGAGGAATGAGACAAA+AGG - chr3.4:76471897-76471916 MS.gene044186:CDS 40.0%
! TCAAGAGGTATAGAAAGAGG+AGG - chr3.4:76472436-76472455 MS.gene044186:CDS 40.0%
!! CATACCTTTGGATTGGTACA+TGG + chr3.4:76471759-76471778 None:intergenic 40.0%
TGCAAATGCAGGTGTGGATG+TGG - chr3.4:76471875-76471894 MS.gene044186:CDS 50.0%
! TCGTTCGTGCTCGACGATTT+TGG + chr3.4:76472139-76472158 None:intergenic 50.0%
CAGGTGTGGATGTGGATCAG+AGG - chr3.4:76471883-76471902 MS.gene044186:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 76471713 76472690 76471713 ID=MS.gene044186
chr3.4 mRNA 76471713 76472690 76471713 ID=MS.gene044186.t1;Parent=MS.gene044186
chr3.4 exon 76472630 76472690 76472630 ID=MS.gene044186.t1.exon1;Parent=MS.gene044186.t1
chr3.4 CDS 76472630 76472690 76472630 ID=cds.MS.gene044186.t1;Parent=MS.gene044186.t1
chr3.4 exon 76472138 76472517 76472138 ID=MS.gene044186.t1.exon2;Parent=MS.gene044186.t1
chr3.4 CDS 76472138 76472517 76472138 ID=cds.MS.gene044186.t1;Parent=MS.gene044186.t1
chr3.4 exon 76471713 76472039 76471713 ID=MS.gene044186.t1.exon3;Parent=MS.gene044186.t1
chr3.4 CDS 76471713 76472039 76471713 ID=cds.MS.gene044186.t1;Parent=MS.gene044186.t1
Gene Sequence

>MS.gene044186

ATGAAGAGGTTCAATAACTCAGAAACTATGGCTTCTTTGCTTTCCATGTACCAATCCAAAGGTATGTATAAGTAATTAAATTACTAATTGTTTCATTCTTCATATACCCTTTTTACCATAATTTATGTGTGTATTTTTGTGGTCTAATTATGTTTCATTCTTTGCAAATGCAGGTGTGGATGTGGATCAGAGGAATGAGACAAAAGGTTACAGTGAAGAATTTCAAGCAATGTTAGATAGATTAGAGCAAGAAGATAGCTATGAAGATGGTAGTTCAATGTTGGAGAAGAAAAGAAGACTTGGTTATGATCAAGTAAAAGCTTTAGAAAAGAGCTTTGAATTGGACAACAAGCTGGAGCCAGAGAGAAAATTGAAGTTAGCTGAAGAATTAGGCTTGCAACCTAGACAAGTTTCAATTTGGTTCCAAAATCGTCGAGCACGAACGAAAACCAAACAATTGGAAAGAGATTATAGTGTTCTTAAGTCCAATTTTGATGTTCTTAAGGTTGAGTATAGTACTCTTCAACAAGAGAATGAGACTCTTACTAGAAAGGTAATAATATATACATGTATTATTCATGATTTTTTATTTATTTATGTGAATAATGTTAATGTGTAATCTTAGTGTCTTTTTATGTGTGTGAGCTGCAGTTAAGAGAGTTGAAAGCGAAATTGTTAAGAGTTGAAAGCAGTGAGTTAGAGAAAGAAGTAGAATGTCCAATTTCAAGAGGTATAGAAAGAGGAGGTTCAGAAAGTAATTCAAGTGGTGTGATTAAGGAAGAAAGTAATGTTGATTCTCCTATATGTTTCAATGGTTCTTTGCCAAGTTCAATGATGAATTTGGTTCATTATTATGATTCAAGAGGTTCTCAAGAAAATGTGTTTCAGAATCAATTTATGAGAATGGAGGAGCAGAATTTCTTAAGTAATAGTGAATTATTCAGTTTCTTTTCAGTTGATCAATCCCCTACTTTGTAA

Protein sequence

>MS.gene044186.t1

MKRFNNSETMASLLSMYQSKGVDVDQRNETKGYSEEFQAMLDRLEQEDSYEDGSSMLEKKRRLGYDQVKALEKSFELDNKLEPERKLKLAEELGLQPRQVSIWFQNRRARTKTKQLERDYSVLKSNFDVLKVEYSTLQQENETLTRKLRELKAKLLRVESSELEKEVECPISRGIERGGSESNSSGVIKEESNVDSPICFNGSLPSSMMNLVHYYDSRGSQENVFQNQFMRMEEQNFLSNSELFSFFSVDQSPTL