Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044279.t1 | KEH35169.1 | 89.3 | 122 | 12 | 1 | 1 | 121 | 52 | 173 | 1.90E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044279.t1 | A0A072UZD5 | 89.3 | 122 | 12 | 1 | 1 | 121 | 52 | 173 | 1.4e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044279.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044279.t1 | MTR_3g081210 | 89.344 | 122 | 12 | 1 | 1 | 121 | 52 | 173 | 2.45e-74 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAACGAAATTGTTGTT+TGG | 0.350812 | 3.3:-68771109 | MS.gene044279:CDS |
| TGCAGGCTAGAGCTATAATA+TGG | 0.402146 | 3.3:-68770560 | MS.gene044279:intron |
| ATTTGACGAAAAGGAGCTTC+AGG | 0.413840 | 3.3:-68770909 | MS.gene044279:intron |
| ATGGTTGGCAAGTCAAGAAA+TGG | 0.426643 | 3.3:-68770541 | MS.gene044279:CDS |
| TCCTATTCTTTCTCTTCTCA+AGG | 0.460409 | 3.3:+68770400 | None:intergenic |
| CAAGAAATGGATGGAAAGAA+TGG | 0.480394 | 3.3:-68770528 | MS.gene044279:CDS |
| ATGCACTAGGTTGCATTGAA+AGG | 0.491779 | 3.3:+68770475 | None:intergenic |
| TGCACTAGGTTGCATTGAAA+GGG | 0.509370 | 3.3:+68770476 | None:intergenic |
| GGCTAGAGCTATAATATGGT+TGG | 0.541963 | 3.3:-68770556 | MS.gene044279:CDS |
| TCCTTGAGAAGAGAAAGAAT+AGG | 0.545631 | 3.3:-68770401 | MS.gene044279:CDS |
| ACACCACAGATTTGACGAAA+AGG | 0.545745 | 3.3:-68770918 | MS.gene044279:CDS |
| TGCATTGAAAGGGATAGTGT+TGG | 0.550068 | 3.3:+68770486 | None:intergenic |
| TTACAAATGCATGCCTCACA+TGG | 0.582863 | 3.3:-68770453 | MS.gene044279:CDS |
| CTTGTTGCAAATCCTCGCAA+TGG | 0.611244 | 3.3:-68770941 | MS.gene044279:CDS |
| TATCATGAGTTACAATAGAG+AGG | 0.622766 | 3.3:+68770364 | None:intergenic |
| AATCTGTGGTGTCCATTGCG+AGG | 0.627615 | 3.3:+68770929 | None:intergenic |
| TTGGCAAGTCAAGAAATGGA+TGG | 0.632410 | 3.3:-68770537 | MS.gene044279:CDS |
| GCATGCATTTGTAATGCACT+AGG | 0.632578 | 3.3:+68770462 | None:intergenic |
| CTTGATGGAAAAGCCATGTG+AGG | 0.674355 | 3.3:+68770440 | None:intergenic |
| CATGAGTTACAATAGAGAGG+TGG | 0.687315 | 3.3:+68770367 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATATCTATTTTTATCAT+TGG | - | chr3.3:68770851-68770870 | MS.gene044279:intron | 10.0% |
| !! | ATTTACATTATGAAAAAGAT+AGG | + | chr3.3:68770828-68770847 | None:intergenic | 15.0% |
| !!! | TTTTATCATTGGTTAAATTT+TGG | - | chr3.3:68770862-68770881 | MS.gene044279:intron | 15.0% |
| !!! | TCTTGGAATTTTTTTTTTGT+AGG | - | chr3.3:68770455-68770474 | MS.gene044279:CDS | 20.0% |
| ! | AAAAAGATAGGTATATTCAC+TGG | + | chr3.3:68770816-68770835 | None:intergenic | 25.0% |
| ! | GTTATACATTGATCATTACA+CGG | + | chr3.3:68770750-68770769 | None:intergenic | 25.0% |
| !! | AAATTTTCTTCCACAAAACA+AGG | + | chr3.3:68770607-68770626 | None:intergenic | 25.0% |
| !! | TATGGTTTTTGTTAAACTCT+TGG | - | chr3.3:68770438-68770457 | MS.gene044279:CDS | 25.0% |
| !!! | TTGTTTTGTGGAAGAAAATT+TGG | - | chr3.3:68770606-68770625 | MS.gene044279:intron | 25.0% |
| ATTATCGCATGCAAAAAGTT+AGG | + | chr3.3:68770650-68770669 | None:intergenic | 30.0% | |
| GATAGAATTTGAGTCGTTTA+TGG | - | chr3.3:68770420-68770439 | MS.gene044279:CDS | 30.0% | |
| GTGTATGGCAAAAAAAAACA+TGG | - | chr3.3:68770673-68770692 | MS.gene044279:intron | 30.0% | |
| TATCATGAGTTACAATAGAG+AGG | + | chr3.3:68771085-68771104 | None:intergenic | 30.0% | |
| !!! | ATTGGTTAAATTTTGGTTGC+AGG | - | chr3.3:68770869-68770888 | MS.gene044279:intron | 30.0% |
| !!! | ATTTCTTCATCCTTGTTTTG+TGG | - | chr3.3:68770594-68770613 | MS.gene044279:intron | 30.0% |
| CAAGAAATGGATGGAAAGAA+TGG | - | chr3.3:68770918-68770937 | MS.gene044279:CDS | 35.0% | |
| TCCTATTCTTTCTCTTCTCA+AGG | + | chr3.3:68771049-68771068 | None:intergenic | 35.0% | |
| TCCTTGAGAAGAGAAAGAAT+AGG | - | chr3.3:68771045-68771064 | MS.gene044279:intron | 35.0% | |
| !!! | ATTTTGAAGTGATCGCTTGA+TGG | + | chr3.3:68771024-68771043 | None:intergenic | 35.0% |
| ACACCACAGATTTGACGAAA+AGG | - | chr3.3:68770528-68770547 | MS.gene044279:CDS | 40.0% | |
| ACATTGATCATTACACGGTG+TGG | + | chr3.3:68770745-68770764 | None:intergenic | 40.0% | |
| ATGCACTAGGTTGCATTGAA+AGG | + | chr3.3:68770974-68770993 | None:intergenic | 40.0% | |
| ATGGTTGGCAAGTCAAGAAA+TGG | - | chr3.3:68770905-68770924 | MS.gene044279:intron | 40.0% | |
| ATTTGACGAAAAGGAGCTTC+AGG | - | chr3.3:68770537-68770556 | MS.gene044279:CDS | 40.0% | |
| CATGAGTTACAATAGAGAGG+TGG | + | chr3.3:68771082-68771101 | None:intergenic | 40.0% | |
| GCATGCATTTGTAATGCACT+AGG | + | chr3.3:68770987-68771006 | None:intergenic | 40.0% | |
| GCATGCGATAATTACGTGTA+TGG | - | chr3.3:68770658-68770677 | MS.gene044279:intron | 40.0% | |
| GGCTAGAGCTATAATATGGT+TGG | - | chr3.3:68770890-68770909 | MS.gene044279:intron | 40.0% | |
| TGCACTAGGTTGCATTGAAA+GGG | + | chr3.3:68770973-68770992 | None:intergenic | 40.0% | |
| TGCAGGCTAGAGCTATAATA+TGG | - | chr3.3:68770886-68770905 | MS.gene044279:intron | 40.0% | |
| TGCATTGAAAGGGATAGTGT+TGG | + | chr3.3:68770963-68770982 | None:intergenic | 40.0% | |
| TTACAAATGCATGCCTCACA+TGG | - | chr3.3:68770993-68771012 | MS.gene044279:intron | 40.0% | |
| TTGGCAAGTCAAGAAATGGA+TGG | - | chr3.3:68770909-68770928 | MS.gene044279:intron | 40.0% | |
| ATCATTACACGGTGTGGTCA+AGG | + | chr3.3:68770739-68770758 | None:intergenic | 45.0% | |
| CTTGATGGAAAAGCCATGTG+AGG | + | chr3.3:68771009-68771028 | None:intergenic | 45.0% | |
| CTTGTTGCAAATCCTCGCAA+TGG | - | chr3.3:68770505-68770524 | MS.gene044279:CDS | 45.0% | |
| TCATTACACGGTGTGGTCAA+GGG | + | chr3.3:68770738-68770757 | None:intergenic | 45.0% | |
| ! | GCTCCTTTTCGTCAAATCTG+TGG | + | chr3.3:68770534-68770553 | None:intergenic | 45.0% |
| AATCTGTGGTGTCCATTGCG+AGG | + | chr3.3:68770520-68770539 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 68770338 | 68771130 | 68770338 | ID=MS.gene044279 |
| chr3.3 | mRNA | 68770338 | 68771130 | 68770338 | ID=MS.gene044279.t1;Parent=MS.gene044279 |
| chr3.3 | exon | 68771087 | 68771130 | 68771087 | ID=MS.gene044279.t1.exon1;Parent=MS.gene044279.t1 |
| chr3.3 | CDS | 68771087 | 68771130 | 68771087 | ID=cds.MS.gene044279.t1;Parent=MS.gene044279.t1 |
| chr3.3 | exon | 68770910 | 68770991 | 68770910 | ID=MS.gene044279.t1.exon2;Parent=MS.gene044279.t1 |
| chr3.3 | CDS | 68770910 | 68770991 | 68770910 | ID=cds.MS.gene044279.t1;Parent=MS.gene044279.t1 |
| chr3.3 | exon | 68770338 | 68770577 | 68770338 | ID=MS.gene044279.t1.exon3;Parent=MS.gene044279.t1 |
| chr3.3 | CDS | 68770338 | 68770577 | 68770338 | ID=cds.MS.gene044279.t1;Parent=MS.gene044279.t1 |
| Gene Sequence |
| Protein sequence |
