Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044288.t1 | XP_024634747.1 | 96.6 | 499 | 17 | 0 | 1 | 499 | 36 | 534 | 1.90E-261 | 911.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044288.t1 | Q9SFD5 | 47.9 | 509 | 235 | 8 | 7 | 496 | 47 | 544 | 6.1e-120 | 432.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044288.t1 | A0A072V1B9 | 93.2 | 502 | 19 | 2 | 1 | 499 | 24 | 513 | 2.5e-247 | 864.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044288.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044288.t1 | MTR_3g081360 | 93.825 | 502 | 16 | 2 | 1 | 499 | 24 | 513 | 0.0 | 964 |
| MS.gene044288.t1 | MTR_3g082790 | 80.115 | 523 | 79 | 3 | 1 | 498 | 41 | 563 | 0.0 | 838 |
| MS.gene044288.t1 | MTR_3g082790 | 80.115 | 523 | 79 | 3 | 1 | 498 | 1 | 523 | 0.0 | 837 |
| MS.gene044288.t1 | MTR_2g026310 | 50.294 | 511 | 237 | 6 | 2 | 496 | 39 | 548 | 7.35e-168 | 485 |
| MS.gene044288.t1 | MTR_7g063290 | 47.648 | 489 | 208 | 7 | 7 | 495 | 43 | 483 | 4.82e-145 | 425 |
| MS.gene044288.t1 | MTR_2g026380 | 46.263 | 495 | 215 | 7 | 7 | 496 | 44 | 492 | 1.28e-140 | 414 |
| MS.gene044288.t1 | MTR_3g081380 | 77.852 | 149 | 31 | 1 | 1 | 147 | 35 | 183 | 2.16e-82 | 253 |
| MS.gene044288.t1 | MTR_3g081420 | 37.500 | 208 | 121 | 2 | 295 | 496 | 2 | 206 | 2.22e-40 | 145 |
| MS.gene044288.t1 | MTR_3g081390 | 78.889 | 90 | 19 | 0 | 53 | 142 | 36 | 125 | 2.83e-40 | 141 |
| MS.gene044288.t1 | MTR_7g062030 | 69.811 | 53 | 16 | 0 | 7 | 59 | 57 | 109 | 3.53e-22 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044288.t1 | AT3G07740 | 51.544 | 518 | 218 | 9 | 1 | 496 | 17 | 523 | 1.19e-167 | 484 |
| MS.gene044288.t1 | AT3G07740 | 51.644 | 517 | 217 | 9 | 2 | 496 | 39 | 544 | 2.96e-167 | 484 |
| MS.gene044288.t1 | AT4G16420 | 50.816 | 490 | 187 | 7 | 11 | 498 | 45 | 482 | 1.22e-165 | 477 |
| MS.gene044288.t1 | AT3G07740 | 50.954 | 524 | 217 | 10 | 2 | 496 | 39 | 551 | 1.49e-164 | 477 |
| MS.gene044288.t1 | AT4G16420 | 50.405 | 494 | 187 | 8 | 11 | 498 | 45 | 486 | 1.13e-163 | 473 |
| MS.gene044288.t1 | AT4G16420 | 50.000 | 494 | 188 | 7 | 11 | 498 | 45 | 485 | 6.79e-163 | 470 |
| MS.gene044288.t1 | AT3G07740 | 51.553 | 483 | 204 | 8 | 33 | 496 | 2 | 473 | 1.27e-154 | 449 |
Find 90 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTAACTCCATCATTATTT+TGG | 0.150007 | 3.3:-68893412 | None:intergenic |
| TTTGGCAGAAATGGAATTTA+AGG | 0.163949 | 3.3:+68894836 | MS.gene044288:CDS |
| GTAGGATTTGTCTTATTCTA+TGG | 0.220086 | 3.3:+68892095 | MS.gene044288:intron |
| TATGATTTGCTTGTGGAAAA+AGG | 0.230023 | 3.3:+68896622 | MS.gene044288:CDS |
| CAAAAGAATAAAAGACTTAA+AGG | 0.230732 | 3.3:+68895441 | MS.gene044288:CDS |
| GAAAAGAAGCCTAAATTATC+TGG | 0.247074 | 3.3:+68894723 | MS.gene044288:CDS |
| GCTATTGACATGCATGGATT+TGG | 0.276427 | 3.3:+68891886 | MS.gene044288:CDS |
| GTTCACATTGGAAGCCTTCT+TGG | 0.280186 | 3.3:-68893390 | None:intergenic |
| TGCATAGAGTGCTTCTTTGT+TGG | 0.282569 | 3.3:+68889809 | MS.gene044288:CDS |
| TGGATATGCATCAGATTTCT+TGG | 0.286020 | 3.3:-68896563 | None:intergenic |
| ACATGCATGGATTTGGGAAT+TGG | 0.309773 | 3.3:+68891893 | MS.gene044288:CDS |
| CAATGATGCTGAGCAAGTTT+TGG | 0.319202 | 3.3:+68894818 | MS.gene044288:CDS |
| TTTAATACCATAACTCGATA+TGG | 0.332731 | 3.3:-68889863 | None:intergenic |
| TCACATATTTGAAGCTCTTC+GGG | 0.342820 | 3.3:-68895334 | None:intergenic |
| TTCACATATTTGAAGCTCTT+CGG | 0.343334 | 3.3:-68895335 | None:intergenic |
| TTATATTCCCCAGATAATTT+AGG | 0.358245 | 3.3:-68894732 | None:intergenic |
| CCAAAATAATGATGGAGTTA+AGG | 0.371137 | 3.3:+68893412 | MS.gene044288:CDS |
| CTATTGACATGCATGGATTT+GGG | 0.372854 | 3.3:+68891887 | MS.gene044288:CDS |
| TCTTCACCTGCACTCCAATC+TGG | 0.373150 | 3.3:-68891714 | None:intergenic |
| TGTCTTTGTGATAGCTTGTA+TGG | 0.382689 | 3.3:-68896222 | None:intergenic |
| AGAGTCAGCTTTGCATTTGA+AGG | 0.391333 | 3.3:-68893079 | None:intergenic |
| TATGGCTGGCGGTGCATCCT+TGG | 0.406764 | 3.3:-68896204 | None:intergenic |
| TCGCAGGAAGCTAGAATTGC+TGG | 0.412483 | 3.3:+68896056 | MS.gene044288:intron |
| ATCAATTTATTGAACAAAAG+AGG | 0.414539 | 3.3:+68896102 | MS.gene044288:CDS |
| GGTGAGAGAAATTTCTCAAA+AGG | 0.423818 | 3.3:-68895313 | None:intergenic |
| TATAAAGAAAGTGGTCAGAT+TGG | 0.427378 | 3.3:+68896149 | MS.gene044288:CDS |
| GAAGCCTTCTTGGTTATACT+TGG | 0.427656 | 3.3:-68893380 | None:intergenic |
| TAAATTCTGTCAATCTTGCT+TGG | 0.438413 | 3.3:-68896601 | None:intergenic |
| CACAAAGACACTAGAAGAAT+GGG | 0.442742 | 3.3:+68896235 | MS.gene044288:CDS |
| CCTCTCAACACTTCAATAGA+TGG | 0.448042 | 3.3:-68894756 | None:intergenic |
| TAGGATTTGTCTTATTCTAT+GGG | 0.455897 | 3.3:+68892096 | MS.gene044288:intron |
| TGAAGAGAAGTTACTTATTG+AGG | 0.462048 | 3.3:+68891731 | MS.gene044288:CDS |
| CTTTAGGCTATTGACATGCA+TGG | 0.463532 | 3.3:+68891880 | MS.gene044288:intron |
| GAACTCCTTGCCATGGCAAA+AGG | 0.466099 | 3.3:+68892132 | MS.gene044288:CDS |
| TCACAAAGACACTAGAAGAA+TGG | 0.467289 | 3.3:+68896234 | MS.gene044288:CDS |
| AGAAATTTCTCAAAAGGATC+GGG | 0.467873 | 3.3:-68895307 | None:intergenic |
| TCAGATATACAGCATTATAA+TGG | 0.471582 | 3.3:-68891966 | None:intergenic |
| TTCCGCTTACATCTCCAGAT+TGG | 0.472380 | 3.3:+68891700 | MS.gene044288:CDS |
| AATGATGGAGTTAAGGTTGA+AGG | 0.474514 | 3.3:+68893419 | MS.gene044288:CDS |
| TCTCAAGCAGGTTGGAGCAC+TGG | 0.475857 | 3.3:+68894699 | MS.gene044288:CDS |
| CCGCGTAATGTTGAGGAAGA+TGG | 0.476953 | 3.3:-68896506 | None:intergenic |
| AAAAGAAGCCTAAATTATCT+GGG | 0.481337 | 3.3:+68894724 | MS.gene044288:CDS |
| CAATCATCCATATCGAGTTA+TGG | 0.484056 | 3.3:+68889856 | MS.gene044288:CDS |
| GGCGGTGCATCCTTGGTACT+AGG | 0.496097 | 3.3:-68896197 | None:intergenic |
| TTTACTTTATTCCATCAGAA+AGG | 0.497295 | 3.3:-68893114 | None:intergenic |
| AAAGAACCTCACCTTTCTGA+TGG | 0.498619 | 3.3:+68893103 | MS.gene044288:CDS |
| GAGAAATTTCTCAAAAGGAT+CGG | 0.500929 | 3.3:-68895308 | None:intergenic |
| TTTGTGATAGCTTGTATGGC+TGG | 0.504362 | 3.3:-68896218 | None:intergenic |
| AATGTGAACCAAAATAATGA+TGG | 0.504833 | 3.3:+68893404 | MS.gene044288:CDS |
| CGCGTAATGTTGAGGAAGAT+GGG | 0.509064 | 3.3:-68896505 | None:intergenic |
| ACATCTCCAGATTGGAGTGC+AGG | 0.509957 | 3.3:+68891708 | MS.gene044288:CDS |
| TCAAATAGCCTTACAGGTAG+AGG | 0.513546 | 3.3:-68892003 | None:intergenic |
| TCAAATATGTGAACAGTACA+AGG | 0.524557 | 3.3:+68895345 | MS.gene044288:CDS |
| CATGCAGTTAGAAATTTCAA+AGG | 0.524634 | 3.3:+68896531 | MS.gene044288:CDS |
| CTATAGATTGTACCTGAGGT+TGG | 0.533420 | 3.3:-68893257 | None:intergenic |
| CTTGGAGAGAACTTGAACAG+TGG | 0.542544 | 3.3:-68896583 | None:intergenic |
| ATGCAGTTAGAAATTTCAAA+GGG | 0.542710 | 3.3:+68896532 | MS.gene044288:CDS |
| CAGCTCTGAAGAATCTAAGA+AGG | 0.544590 | 3.3:+68893205 | MS.gene044288:intron |
| CAAGGTAACTATAAAGAAAG+TGG | 0.546753 | 3.3:+68896140 | MS.gene044288:CDS |
| AAAGGACATCAACTCAAGAA+AGG | 0.549772 | 3.3:+68892150 | MS.gene044288:CDS |
| CAGAATTTATGATTTGCTTG+TGG | 0.553524 | 3.3:+68896615 | MS.gene044288:CDS |
| TCTTCCAAGTATAACCAAGA+AGG | 0.569053 | 3.3:+68893376 | MS.gene044288:CDS |
| ATGGTGTTGCTGAAAACGTT+GGG | 0.572743 | 3.3:+68891917 | MS.gene044288:CDS |
| AACATGTCAAGGCCAACCTC+AGG | 0.574063 | 3.3:+68893245 | MS.gene044288:CDS |
| TTGTTAGTAGAATCTCAAGC+AGG | 0.574478 | 3.3:+68894687 | MS.gene044288:intron |
| AGTGGTCAGATTGGAACAAG+TGG | 0.587270 | 3.3:+68896158 | MS.gene044288:CDS |
| CATCTATTGAAGTGTTGAGA+GGG | 0.593154 | 3.3:+68894757 | MS.gene044288:CDS |
| GCATGATCCGCGTAATGTTG+AGG | 0.597253 | 3.3:-68896513 | None:intergenic |
| CATGGATTTGGGAATTGGAA+TGG | 0.599035 | 3.3:+68891898 | MS.gene044288:CDS |
| CCACTAATCAAAACATGTCA+AGG | 0.601102 | 3.3:+68893234 | MS.gene044288:CDS |
| TAGTAGAATCTCAAGCAGGT+TGG | 0.601506 | 3.3:+68894691 | MS.gene044288:intron |
| CCATCTATTGAAGTGTTGAG+AGG | 0.602727 | 3.3:+68894756 | MS.gene044288:CDS |
| CTTACAGGTAGAGGAAAACA+TGG | 0.611599 | 3.3:-68891994 | None:intergenic |
| CCATCTTCCTCAACATTACG+CGG | 0.613654 | 3.3:+68896506 | MS.gene044288:CDS |
| TGAACGTGAAATGAAACTGC+AGG | 0.617265 | 3.3:+68894875 | MS.gene044288:CDS |
| CAAAGAGGAACTCCTTGCCA+TGG | 0.618565 | 3.3:+68892125 | MS.gene044288:CDS |
| AGAATCATTTCCTAGTACCA+AGG | 0.625963 | 3.3:+68896187 | MS.gene044288:CDS |
| TTTATTCCATCAGAAAGGTG+AGG | 0.628847 | 3.3:-68893109 | None:intergenic |
| AGGACAAAAGAAGCTGAACA+AGG | 0.637958 | 3.3:+68896122 | MS.gene044288:CDS |
| TCACTCAAAAGAAGAGCATG+AGG | 0.651691 | 3.3:+68895381 | MS.gene044288:CDS |
| TTCTATGGGAAAGAACAAAG+AGG | 0.651784 | 3.3:+68892110 | MS.gene044288:CDS |
| GTGATAGCTTGTATGGCTGG+CGG | 0.658411 | 3.3:-68896215 | None:intergenic |
| AATGGTGTTGCTGAAAACGT+TGG | 0.659458 | 3.3:+68891916 | MS.gene044288:CDS |
| AACAGTACAAGGTCTTCATG+CGG | 0.668099 | 3.3:+68895356 | MS.gene044288:CDS |
| CTCCAATCTGGAGATGTAAG+CGG | 0.681010 | 3.3:-68891702 | None:intergenic |
| AAAGAAGCCTAAATTATCTG+GGG | 0.686550 | 3.3:+68894725 | MS.gene044288:CDS |
| AGAGGGTACAATTCTAACAG+AGG | 0.689534 | 3.3:+68894774 | MS.gene044288:CDS |
| GTAGGCATGGGAATAACTGA+TGG | 0.696306 | 3.3:+68889695 | None:intergenic |
| ATGAAACTGCAGGTGCTCCG+TGG | 0.711759 | 3.3:+68894885 | MS.gene044288:CDS |
| GTAATGTTGAGGAAGATGGG+TGG | 0.746783 | 3.3:-68896502 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 68889701 | 68896660 | 68889701 | ID=MS.gene044288 |
| chr3.3 | mRNA | 68889701 | 68896660 | 68889701 | ID=MS.gene044288.t1;Parent=MS.gene044288 |
| chr3.3 | exon | 68889701 | 68889877 | 68889701 | ID=MS.gene044288.t1.exon1;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68889701 | 68889877 | 68889701 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68891687 | 68891752 | 68891687 | ID=MS.gene044288.t1.exon2;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68891687 | 68891752 | 68891687 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68891886 | 68892011 | 68891886 | ID=MS.gene044288.t1.exon3;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68891886 | 68892011 | 68891886 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68892099 | 68892171 | 68892099 | ID=MS.gene044288.t1.exon4;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68892099 | 68892171 | 68892099 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68893074 | 68893131 | 68893074 | ID=MS.gene044288.t1.exon5;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68893074 | 68893131 | 68893074 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68893208 | 68893266 | 68893208 | ID=MS.gene044288.t1.exon6;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68893208 | 68893266 | 68893208 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68893363 | 68893440 | 68893363 | ID=MS.gene044288.t1.exon7;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68893363 | 68893440 | 68893363 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68894697 | 68894919 | 68894697 | ID=MS.gene044288.t1.exon8;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68894697 | 68894919 | 68894697 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68895252 | 68895462 | 68895252 | ID=MS.gene044288.t1.exon9;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68895252 | 68895462 | 68895252 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68896062 | 68896298 | 68896062 | ID=MS.gene044288.t1.exon10;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68896062 | 68896298 | 68896062 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| chr3.3 | exon | 68896469 | 68896660 | 68896469 | ID=MS.gene044288.t1.exon11;Parent=MS.gene044288.t1 |
| chr3.3 | CDS | 68896469 | 68896660 | 68896469 | ID=cds.MS.gene044288.t1;Parent=MS.gene044288.t1 |
| Gene Sequence |
| Protein sequence |
