AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044391

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044391.t1 MTR_4g108720 97.472 356 9 0 1 356 1 356 0.0 721
MS.gene044391.t1 MTR_5g041650 49.587 363 163 8 1 354 38 389 1.74e-109 326
MS.gene044391.t1 MTR_3g102570 36.872 179 102 5 1 173 1 174 1.57e-28 113
MS.gene044391.t1 MTR_4g084740 33.188 229 126 7 1 214 1 217 7.85e-26 106
MS.gene044391.t1 MTR_5g066180 34.302 172 79 5 1 169 1 141 8.96e-18 81.6
MS.gene044391.t1 MTR_1g029670 39.423 104 63 0 1 104 1 104 6.67e-17 78.2
MS.gene044391.t1 MTR_3g088615 62.264 53 20 0 1 53 1 53 3.77e-16 76.3
MS.gene044391.t1 MTR_1g053070 48.611 72 35 1 1 72 1 70 6.95e-16 77.0
MS.gene044391.t1 MTR_5g032520 48.571 70 34 1 1 70 1 68 8.04e-15 73.6
MS.gene044391.t1 MTR_5g032150 48.571 70 34 1 1 70 1 68 8.83e-15 73.2
MS.gene044391.t1 MTR_0003s0590 46.479 71 36 1 1 71 1 69 9.15e-15 73.9
MS.gene044391.t1 MTR_3g113030 56.604 53 23 0 1 53 1 53 1.21e-14 72.8
MS.gene044391.t1 MTR_5g046790 45.570 79 42 1 1 79 1 78 1.32e-14 72.4
MS.gene044391.t1 MTR_2g017865 45.070 71 37 1 1 71 16 84 1.39e-14 73.6
MS.gene044391.t1 MTR_8g066260 43.038 79 44 1 1 79 1 78 1.95e-14 72.4
MS.gene044391.t1 MTR_7g075870 30.899 178 93 5 1 176 1 150 2.02e-14 72.0
MS.gene044391.t1 MTR_5g066960 50.943 53 26 0 1 53 1 53 2.03e-14 67.8
MS.gene044391.t1 MTR_1g038300 45.070 71 39 0 1 71 1 71 2.24e-14 72.4
MS.gene044391.t1 MTR_2g009890 46.479 71 36 1 1 71 1 69 2.27e-14 73.2
MS.gene044391.t1 MTR_3g452380 54.717 53 24 0 1 53 16 68 3.07e-14 72.0
MS.gene044391.t1 MTR_4g109830 43.038 79 44 1 1 79 1 78 3.08e-14 72.0
MS.gene044391.t1 MTR_2g461710 54.717 53 24 0 1 53 1 53 3.73e-14 67.0
MS.gene044391.t1 MTR_3g005530 54.717 53 24 0 1 53 1 53 3.82e-14 71.2
MS.gene044391.t1 MTR_4g093970 48.571 70 34 1 1 70 27 94 5.81e-14 71.2
MS.gene044391.t1 MTR_5g031000 43.662 71 38 1 1 71 1 69 8.60e-14 70.5
MS.gene044391.t1 MTR_8g087860 52.830 53 25 0 1 53 17 69 9.15e-14 70.9
MS.gene044391.t1 MTR_1g101970 46.575 73 37 1 1 73 1 71 9.17e-14 69.7
MS.gene044391.t1 MTR_5g031000 43.662 71 38 1 1 71 1 69 1.00e-13 70.5
MS.gene044391.t1 MTR_7g075850 41.333 75 44 0 1 75 1 75 1.86e-13 70.1
MS.gene044391.t1 MTR_7g075870 29.775 178 96 5 1 176 1 151 2.55e-13 68.9
MS.gene044391.t1 MTR_5g021270 38.710 124 71 4 1 120 1 123 2.84e-13 68.9
MS.gene044391.t1 MTR_8g097090 32.057 209 106 10 1 188 1 194 2.91e-13 69.3
MS.gene044391.t1 MTR_1g054265 38.961 77 45 1 1 77 1 75 3.14e-13 67.4
MS.gene044391.t1 MTR_5g021270 38.710 124 71 4 1 120 1 123 3.33e-13 68.9
MS.gene044391.t1 MTR_1g108510 29.762 168 104 6 2 167 9 164 3.87e-13 68.6
MS.gene044391.t1 MTR_4g102530 56.604 53 23 0 1 53 1 53 6.02e-13 67.8
MS.gene044391.t1 MTR_3g030770 32.558 129 82 4 2 128 9 134 6.68e-13 67.8
MS.gene044391.t1 MTR_8g033270 52.830 53 25 0 1 53 1 53 6.68e-13 68.2
MS.gene044391.t1 MTR_7g016600 31.343 201 109 8 1 184 1 189 7.51e-13 68.2
MS.gene044391.t1 MTR_5g046870 52.830 53 25 0 1 53 1 53 7.66e-13 65.1
MS.gene044391.t1 MTR_4g109810 50.943 53 26 0 1 53 1 53 9.03e-13 67.8
MS.gene044391.t1 MTR_4g093030 54.717 53 24 0 1 53 1 53 9.08e-13 65.9
MS.gene044391.t1 MTR_8g086290 51.923 52 25 0 2 53 12 63 1.29e-12 65.9
MS.gene044391.t1 MTR_8g043650 28.977 176 103 7 2 167 9 172 1.58e-12 67.0
MS.gene044391.t1 MTR_1g041615 37.363 91 51 2 16 104 20 106 1.78e-12 64.7
MS.gene044391.t1 MTR_8g033220 43.662 71 38 1 1 71 1 69 2.40e-12 66.2
MS.gene044391.t1 MTR_8g033220 50.943 53 26 0 1 53 1 53 2.49e-12 66.2
MS.gene044391.t1 MTR_6g015975 50.943 53 26 0 1 53 1 53 2.76e-12 66.6
MS.gene044391.t1 MTR_3g084980 50.943 53 26 0 1 53 1 53 3.15e-12 66.2
MS.gene044391.t1 MTR_4g094638 53.704 54 25 0 1 54 1 54 3.36e-12 64.7
MS.gene044391.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 3.43e-12 64.7
MS.gene044391.t1 MTR_7g016630 28.977 176 113 4 1 169 1 171 3.72e-12 66.2
MS.gene044391.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 3.88e-12 65.1
MS.gene044391.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 4.70e-12 64.7
MS.gene044391.t1 MTR_8g051580 30.247 162 80 5 1 156 1 135 4.98e-12 64.3
MS.gene044391.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 5.12e-12 64.7
MS.gene044391.t1 MTR_1g108580 38.636 88 51 2 2 89 9 93 6.21e-12 65.1
MS.gene044391.t1 MTR_6g464720 43.243 74 39 2 1 73 1 72 7.36e-12 65.1
MS.gene044391.t1 MTR_3g052870 33.884 121 73 5 2 119 9 125 8.18e-12 64.7
MS.gene044391.t1 MTR_4g131030 40.260 77 44 1 1 77 1 75 1.18e-11 63.9
MS.gene044391.t1 MTR_1g105920 33.884 121 73 5 2 119 9 125 1.39e-11 64.3
MS.gene044391.t1 MTR_2g105290 34.146 123 56 2 1 98 11 133 1.59e-11 62.8
MS.gene044391.t1 MTR_3g052920 39.326 89 49 3 2 89 9 93 1.96e-11 63.9
MS.gene044391.t1 MTR_1g063160 37.662 77 46 1 1 77 1 75 2.67e-11 62.4
MS.gene044391.t1 MTR_8g079502 46.296 54 29 0 1 54 1 54 2.85e-11 62.4
MS.gene044391.t1 MTR_5g045560 39.744 78 45 1 1 78 8 83 3.03e-11 63.2
MS.gene044391.t1 MTR_0121s0100 41.333 75 42 1 2 76 9 81 3.05e-11 60.8
MS.gene044391.t1 MTR_8g046350 30.075 133 66 3 1 133 1 106 3.56e-11 61.6
MS.gene044391.t1 MTR_2g085280 38.043 92 54 2 2 93 9 97 3.56e-11 63.2
MS.gene044391.t1 MTR_2g085250 38.043 92 54 2 2 93 9 97 3.60e-11 63.2
MS.gene044391.t1 MTR_1g105910 38.202 89 50 3 2 89 9 93 3.60e-11 63.2
MS.gene044391.t1 MTR_1g075600 29.293 198 113 10 2 195 9 183 3.77e-11 63.2
MS.gene044391.t1 MTR_2g030740 38.202 89 50 3 2 89 9 93 6.57e-11 62.4
MS.gene044391.t1 MTR_1g047550 46.296 54 29 0 1 54 1 54 7.25e-11 61.2
MS.gene044391.t1 MTR_4g094632 48.148 54 28 0 1 54 1 54 7.41e-11 60.5
MS.gene044391.t1 MTR_1g077360 42.857 77 41 2 2 77 9 83 7.86e-11 62.0
MS.gene044391.t1 MTR_5g055100 42.857 77 41 2 2 77 9 83 8.24e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044391.t1 AT1G18750 52.824 301 123 6 1 296 1 287 3.45e-97 294
MS.gene044391.t1 AT1G18750 52.824 301 123 6 1 296 1 287 8.93e-97 293
MS.gene044391.t1 AT1G18750 47.605 334 123 7 1 296 1 320 4.13e-90 277
MS.gene044391.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.68e-89 273
MS.gene044391.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.68e-89 273
MS.gene044391.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.68e-89 273
MS.gene044391.t1 AT2G03060 51.930 285 113 5 1 276 1 270 4.92e-89 273
MS.gene044391.t1 AT1G69540 38.947 380 186 13 1 355 1 359 5.27e-70 224
MS.gene044391.t1 AT1G69540 46.097 269 134 5 1 260 1 267 7.05e-70 223
MS.gene044391.t1 AT1G18750 46.275 255 118 6 47 296 1 241 6.48e-66 212
MS.gene044391.t1 AT2G03060 43.627 204 91 5 82 276 24 212 2.10e-42 150
MS.gene044391.t1 AT2G03060 43.627 204 91 5 82 276 24 212 2.10e-42 150
MS.gene044391.t1 AT2G03060 43.627 204 91 5 82 276 24 212 2.10e-42 150
MS.gene044391.t1 AT1G18750 36.923 195 104 6 107 296 5 185 6.31e-29 114
MS.gene044391.t1 AT1G77980 36.735 196 108 6 1 181 1 195 4.62e-28 112
MS.gene044391.t1 AT1G18750 36.508 189 101 6 113 296 23 197 1.21e-26 107
MS.gene044391.t1 AT1G22130 33.500 200 118 5 1 186 1 199 2.12e-25 105
MS.gene044391.t1 AT1G77950 34.031 191 101 6 1 171 1 186 8.78e-24 99.0
MS.gene044391.t1 AT1G77950 34.031 191 101 6 1 171 1 186 8.78e-24 99.0
MS.gene044391.t1 AT1G77950 34.031 191 101 6 1 171 1 186 8.78e-24 99.0
MS.gene044391.t1 AT1G77950 34.031 191 101 6 1 171 1 186 8.78e-24 99.0
MS.gene044391.t1 AT1G69540 33.333 174 106 4 96 260 2 174 5.13e-21 91.7
MS.gene044391.t1 AT2G26320 47.561 82 43 0 1 82 17 98 4.47e-19 82.0
MS.gene044391.t1 AT2G03710 36.747 166 70 6 1 159 1 138 2.70e-17 81.3
MS.gene044391.t1 AT2G03710 36.747 166 70 6 1 159 1 138 2.83e-17 81.3
MS.gene044391.t1 AT2G03710 36.747 166 70 6 1 159 1 138 9.70e-17 78.2
MS.gene044391.t1 AT4G24540 32.787 183 91 6 1 180 1 154 4.46e-16 77.0
MS.gene044391.t1 AT5G13790 38.384 99 57 2 1 97 1 97 7.83e-16 77.0
MS.gene044391.t1 AT5G23260 47.887 71 37 0 1 71 1 71 1.98e-15 75.5
MS.gene044391.t1 AT5G23260 47.887 71 37 0 1 71 1 71 2.06e-15 75.9
MS.gene044391.t1 AT5G23260 47.887 71 37 0 1 71 17 87 2.78e-15 75.5
MS.gene044391.t1 AT5G23260 47.887 71 37 0 1 71 17 87 3.06e-15 75.5
MS.gene044391.t1 AT3G57390 42.353 85 47 1 1 85 1 83 3.29e-15 74.7
MS.gene044391.t1 AT3G57390 42.353 85 47 1 1 85 1 83 4.16e-15 74.7
MS.gene044391.t1 AT5G13790 43.056 72 39 1 1 72 1 70 5.09e-15 74.7
MS.gene044391.t1 AT3G57230 44.444 72 38 1 1 72 1 70 8.63e-15 71.2
MS.gene044391.t1 AT4G37435 26.531 196 108 6 1 178 1 178 1.41e-14 72.0
MS.gene044391.t1 AT2G22540 32.927 164 84 6 1 164 1 138 1.84e-14 72.8
MS.gene044391.t1 AT2G22540 32.927 164 84 6 1 164 1 138 1.84e-14 72.8
MS.gene044391.t1 AT1G26310 43.038 79 44 1 1 79 1 78 2.05e-14 72.8
MS.gene044391.t1 AT3G57230 44.444 72 38 1 1 72 1 70 2.41e-14 72.4
MS.gene044391.t1 AT3G57230 44.444 72 38 1 1 72 1 70 2.41e-14 72.4
MS.gene044391.t1 AT2G14210 43.590 78 43 1 1 78 1 77 2.52e-14 72.4
MS.gene044391.t1 AT2G45650 30.000 190 114 6 1 180 1 181 2.60e-14 72.4
MS.gene044391.t1 AT2G14210 43.590 78 43 1 1 78 1 77 2.93e-14 72.0
MS.gene044391.t1 AT4G37940 45.070 71 37 1 1 71 1 69 3.76e-14 70.5
MS.gene044391.t1 AT5G51870 45.333 75 39 1 1 75 1 73 4.65e-14 70.1
MS.gene044391.t1 AT4G37940 45.070 71 37 1 1 71 1 69 4.65e-14 70.9
MS.gene044391.t1 AT1G69120 43.038 79 44 1 1 79 1 78 5.65e-14 71.2
MS.gene044391.t1 AT4G37940 45.070 71 37 1 1 71 1 69 5.89e-14 70.9
MS.gene044391.t1 AT4G09960 43.662 71 38 1 1 71 1 69 6.42e-14 70.9
MS.gene044391.t1 AT1G69120 43.038 79 44 1 1 79 1 78 7.27e-14 71.2
MS.gene044391.t1 AT2G45660 46.479 71 36 1 1 71 1 69 7.78e-14 68.9
MS.gene044391.t1 AT5G51870 45.333 75 39 1 1 75 1 73 8.85e-14 70.1
MS.gene044391.t1 AT2G22540 52.830 53 25 0 1 53 1 53 8.91e-14 70.5
MS.gene044391.t1 AT4G09960 43.662 71 38 1 1 71 1 69 9.10e-14 70.5
MS.gene044391.t1 AT4G09960 43.662 71 38 1 1 71 2 70 1.01e-13 70.5
MS.gene044391.t1 AT4G09960 43.662 71 38 1 1 71 11 79 1.12e-13 70.5
MS.gene044391.t1 AT2G45660 46.479 71 36 1 1 71 1 69 1.17e-13 69.7
MS.gene044391.t1 AT3G58780 52.830 53 25 0 1 53 16 68 1.18e-13 70.5
MS.gene044391.t1 AT5G51870 45.333 75 39 1 1 75 24 96 1.19e-13 69.3
MS.gene044391.t1 AT1G71692 42.500 80 45 1 1 80 1 79 1.35e-13 69.7
MS.gene044391.t1 AT3G58780 52.830 53 25 0 1 53 16 68 1.40e-13 70.5
MS.gene044391.t1 AT2G42830 52.830 53 25 0 1 53 16 68 1.46e-13 70.1
MS.gene044391.t1 AT5G51870 45.333 75 39 1 1 75 1 73 1.55e-13 69.7
MS.gene044391.t1 AT2G42830 52.830 53 25 0 1 53 16 68 1.59e-13 70.1
MS.gene044391.t1 AT3G58780 52.830 53 25 0 1 53 22 74 1.65e-13 70.1
MS.gene044391.t1 AT5G51870 45.333 75 39 1 1 75 11 83 1.79e-13 69.7
MS.gene044391.t1 AT4G09960 43.662 71 38 1 1 71 27 95 1.99e-13 70.1
MS.gene044391.t1 AT3G58780 52.830 53 25 0 1 53 16 68 2.38e-13 70.1
MS.gene044391.t1 AT4G18960 53.846 52 24 0 2 53 18 69 2.52e-13 69.7
MS.gene044391.t1 AT5G20240 36.782 87 55 0 1 87 1 87 2.86e-13 67.4
MS.gene044391.t1 AT3G58780 52.830 53 25 0 1 53 16 68 3.41e-13 69.7
MS.gene044391.t1 AT2G22630 43.750 80 41 2 1 78 1 78 3.70e-13 68.6
MS.gene044391.t1 AT2G22630 43.750 80 41 2 1 78 1 78 3.70e-13 68.6
MS.gene044391.t1 AT3G61120 56.604 53 23 0 1 53 1 53 4.01e-13 68.9
MS.gene044391.t1 AT5G60910 45.070 71 38 1 1 71 1 70 4.37e-13 68.9
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 4.78e-13 65.5
MS.gene044391.t1 AT5G20240 50.943 53 26 0 1 53 1 53 4.82e-13 68.2
MS.gene044391.t1 AT4G09960 52.830 53 25 0 1 53 96 148 5.18e-13 69.7
MS.gene044391.t1 AT5G10140 52.830 53 25 0 1 53 1 53 5.29e-13 67.0
MS.gene044391.t1 AT3G54340 31.731 104 71 0 1 104 1 104 5.51e-13 68.2
MS.gene044391.t1 AT3G30260 40.845 71 41 1 1 71 1 70 5.92e-13 68.6
MS.gene044391.t1 AT5G10140 52.830 53 25 0 1 53 1 53 6.36e-13 67.4
MS.gene044391.t1 AT5G10140 52.830 53 25 0 1 53 1 53 6.82e-13 67.0
MS.gene044391.t1 AT5G10140 52.830 53 25 0 1 53 1 53 6.84e-13 67.0
MS.gene044391.t1 AT4G11880 52.830 53 25 0 1 53 1 53 1.32e-12 66.2
MS.gene044391.t1 AT4G11880 52.830 53 25 0 1 53 1 53 1.32e-12 66.2
MS.gene044391.t1 AT4G11880 52.830 53 25 0 1 53 1 53 2.38e-12 66.2
MS.gene044391.t1 AT4G11880 52.830 53 25 0 1 53 1 53 2.38e-12 66.2
MS.gene044391.t1 AT1G24260 50.943 53 26 0 1 53 1 53 2.56e-12 66.6
MS.gene044391.t1 AT5G51860 28.723 188 98 6 1 168 1 172 2.82e-12 65.9
MS.gene044391.t1 AT1G24260 50.943 53 26 0 1 53 1 53 3.00e-12 66.6
MS.gene044391.t1 AT1G24260 50.943 53 26 0 1 53 1 53 3.12e-12 66.2
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.37e-12 64.3
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.37e-12 64.3
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.37e-12 64.3
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.37e-12 64.3
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.37e-12 64.3
MS.gene044391.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.56e-12 64.3
MS.gene044391.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.56e-12 64.3
MS.gene044391.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.56e-12 64.3
MS.gene044391.t1 AT5G15800 50.943 53 26 0 1 53 1 53 3.63e-12 66.2
MS.gene044391.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.71e-12 65.5
MS.gene044391.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.71e-12 65.5
MS.gene044391.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.71e-12 65.5
MS.gene044391.t1 AT4G22950 26.519 181 106 3 1 181 1 154 4.21e-12 65.5
MS.gene044391.t1 AT4G22950 26.519 181 106 3 1 181 1 154 4.21e-12 65.5
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 4.28e-12 65.1
MS.gene044391.t1 AT5G62165 40.789 76 43 1 1 76 1 74 4.28e-12 65.1
MS.gene044391.t1 AT5G15800 50.943 53 26 0 1 53 1 53 4.84e-12 65.9
MS.gene044391.t1 AT3G02310 50.943 53 26 0 1 53 1 53 5.71e-12 64.7
MS.gene044391.t1 AT5G51860 42.466 73 40 1 1 73 1 71 6.95e-12 64.7
MS.gene044391.t1 AT3G02310 50.943 53 26 0 1 53 1 53 7.29e-12 65.5
MS.gene044391.t1 AT5G65050 34.211 152 84 5 1 147 1 141 7.56e-12 63.9
MS.gene044391.t1 AT5G65050 43.662 71 39 1 1 71 1 70 9.06e-12 63.5
MS.gene044391.t1 AT4G22950 50.943 53 26 0 1 53 1 53 9.09e-12 63.5
MS.gene044391.t1 AT2G34440 46.296 54 29 0 1 54 1 54 1.47e-11 62.8
MS.gene044391.t1 AT5G65050 43.662 71 39 1 1 71 1 70 1.93e-11 62.8
MS.gene044391.t1 AT5G65050 43.662 71 39 1 1 71 1 70 1.93e-11 63.2
MS.gene044391.t1 AT5G65070 47.170 53 28 0 1 53 1 53 2.15e-11 62.0
MS.gene044391.t1 AT3G18650 26.667 225 152 5 14 231 29 247 3.29e-11 64.7
MS.gene044391.t1 AT5G65070 47.170 53 28 0 1 53 1 53 3.95e-11 62.4
MS.gene044391.t1 AT3G66656 46.296 54 29 0 1 54 1 54 4.03e-11 62.0
MS.gene044391.t1 AT1G01530 49.057 53 27 0 1 53 6 58 4.29e-11 63.2
MS.gene044391.t1 AT4G36590 43.662 71 38 1 2 72 7 75 4.38e-11 63.2
MS.gene044391.t1 AT5G65070 47.170 53 28 0 1 53 1 53 6.93e-11 61.6
MS.gene044391.t1 AT5G65070 47.170 53 28 0 1 53 1 53 7.15e-11 62.0
MS.gene044391.t1 AT5G65070 47.170 53 28 0 1 53 1 53 7.52e-11 62.0

Find 87 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTGAAGATAAAGAAAT+TGG 0.209657 8.4:-18635716 MS.gene044391:CDS
GTTCTAATTGAGCAGGAAAA+TGG 0.237423 8.4:-18635047 MS.gene044391:intron
TGCACATTTAAGTAAGGATT+TGG 0.267580 8.4:+18633234 None:intergenic
CCAAAATCACTATTTGAAAA+TGG 0.281681 8.4:-18633093 MS.gene044391:CDS
TTGAAAATGGACACCAGTTC+TGG 0.282537 8.4:-18633080 MS.gene044391:CDS
AACCAAAGTTTATGTCATTC+AGG 0.285801 8.4:-18633633 MS.gene044391:intron
CCCTTTCTTGTGGGCTAAGT+TGG 0.290811 8.4:+18635146 None:intergenic
ATTTCACTCCCAAGCTATTC+TGG 0.316741 8.4:-18633351 MS.gene044391:CDS
GGTACCTTGAATGAGTTAAA+TGG 0.322272 8.4:-18633261 MS.gene044391:CDS
GCCCACAAGAAAGGGCTAAA+AGG 0.346530 8.4:-18635138 MS.gene044391:intron
CCAAGCTATTCTGGTTATAC+TGG 0.348682 8.4:-18633342 MS.gene044391:CDS
CGCATTCAAGTGACATTAGT+TGG 0.358372 8.4:+18633854 None:intergenic
ACTTTACCCTTATTGATGTC+TGG 0.362461 8.4:-18633724 MS.gene044391:CDS
TTGTAGACCAGACATCAATA+AGG 0.370210 8.4:+18633717 None:intergenic
TGTAAAGATACAAGATTTCC+TGG 0.370430 8.4:-18634860 MS.gene044391:CDS
CCTTTCTTGTGGGCTAAGTT+GGG 0.379085 8.4:+18635147 None:intergenic
AAGGGTAAAGTCATCCCTTC+TGG 0.386047 8.4:+18633736 None:intergenic
AGGAAAGCCTACGTTATTAC+AGG 0.394098 8.4:-18635575 MS.gene044391:CDS
CACTGTTGCACATTTAAGTA+AGG 0.404816 8.4:+18633228 None:intergenic
CTCTCCACATGTCACTACTT+TGG 0.406741 8.4:-18633295 MS.gene044391:CDS
TCTCAGTTATTGGAGTAATC+TGG 0.414099 8.4:-18634089 MS.gene044391:intron
TCTCCACATGTCACTACTTT+GGG 0.420528 8.4:-18633294 MS.gene044391:CDS
ACATGTCACTACTTTGGGTC+AGG 0.427961 8.4:-18633289 MS.gene044391:CDS
ATCATGATCCAATTTCTTGA+AGG 0.431577 8.4:+18634882 None:intergenic
CCCAACTTAGCCCACAAGAA+AGG 0.433847 8.4:-18635147 MS.gene044391:CDS
CTTGGAACATCTTAGACAAA+TGG 0.436675 8.4:-18634053 MS.gene044391:CDS
GTTCACTAGGTAACATCAAC+TGG 0.439721 8.4:+18633653 None:intergenic
TTGATTTGCTATTGATTGGT+TGG 0.442413 8.4:+18633154 None:intergenic
CACCTGAATGACATAAACTT+TGG 0.443645 8.4:+18633631 None:intergenic
CAATCGATTGTCTATACAAA+AGG 0.445203 8.4:-18634008 MS.gene044391:intron
TTATTTGATTTGCTATTGAT+TGG 0.458324 8.4:+18633150 None:intergenic
TTCACTTCCTCAAGATCTTG+TGG 0.459942 8.4:+18633183 None:intergenic
TGGAAGCTGCAAACTGGAGG+TGG 0.461307 8.4:-18633322 MS.gene044391:CDS
TTCATATTGCAGCAGTCAAA+CGG 0.469120 8.4:-18634756 MS.gene044391:intron
GATCATAATTGTTATTAACT+TGG 0.470376 8.4:+18633118 None:intergenic
AAGAATCTCAACCTTTGTCT+TGG 0.471192 8.4:-18633696 MS.gene044391:CDS
TACAAGATTTCCTGGGTTCA+AGG 0.476006 8.4:-18634852 MS.gene044391:intron
ATTTCCATTTAACTCATTCA+AGG 0.477610 8.4:+18633257 None:intergenic
CAGTATAACCAGAATAGCTT+GGG 0.478016 8.4:+18633343 None:intergenic
TTATACTGGAAGCTGCAAAC+TGG 0.478801 8.4:-18633328 MS.gene044391:CDS
AGATTCTCACTTGATGACTT+AGG 0.481817 8.4:+18634623 None:intergenic
TCCTCAAGATCTTGTGGAGG+AGG 0.493605 8.4:+18633189 None:intergenic
CCAACTTAGCCCACAAGAAA+GGG 0.507371 8.4:-18635146 MS.gene044391:CDS
ACTACTTTGGGTCAGGGAGG+TGG 0.508002 8.4:-18633282 MS.gene044391:CDS
TCTGGAGAAGATCAATAACT+TGG 0.508786 8.4:-18634071 MS.gene044391:CDS
TGTAGACCAGACATCAATAA+GGG 0.511954 8.4:+18633718 None:intergenic
CCAGTATAACCAGAATAGCT+TGG 0.515909 8.4:+18633342 None:intergenic
CATTTGTAGAATTCCAGAAC+TGG 0.522572 8.4:+18633067 None:intergenic
GTCACTTGAATGCGTTAACC+AGG 0.524046 8.4:-18633845 MS.gene044391:intron
CAGTTCTGGAATTCTACAAA+TGG 0.526268 8.4:-18633066 MS.gene044391:CDS
ACTTATTCGAAGCGGAAAAG+TGG 0.527998 8.4:-18635670 MS.gene044391:CDS
ATTGCTATGTATAACGAGAA+TGG 0.533675 8.4:-18633033 MS.gene044391:CDS
GACAAATGGAAGACTCACTC+AGG 0.540615 8.4:-18634039 MS.gene044391:CDS
CCTTTGTCTTGGCTTCTAAG+TGG 0.542337 8.4:-18633685 MS.gene044391:CDS
AAGTGAGAATCTTACAAGCT+CGG 0.547873 8.4:-18634612 MS.gene044391:CDS
CCCTCCTCCACAAGATCTTG+AGG 0.562025 8.4:-18633190 MS.gene044391:CDS
ATAACGAGAATGGTTACCAT+AGG 0.564017 8.4:-18633023 MS.gene044391:CDS
ATTGATTCATAGTTACCAGA+AGG 0.565773 8.4:-18633751 MS.gene044391:intron
CTGTTGGAGAAAACATGAGA+AGG 0.576749 8.4:+18635596 None:intergenic
AATTCTACAAATGGCTCTTG+TGG 0.581991 8.4:-18633057 MS.gene044391:CDS
CTTATTCGAAGCGGAAAAGT+GGG 0.585417 8.4:-18635669 MS.gene044391:CDS
CAATCACTTACCTTGAACCC+AGG 0.589742 8.4:+18634842 None:intergenic
CGTTCTCCCTGTAATAACGT+AGG 0.597125 8.4:+18635568 None:intergenic
GTAAAGATACAAGATTTCCT+GGG 0.597719 8.4:-18634859 MS.gene044391:CDS
TAACGAGAATGGTTACCATA+GGG 0.597927 8.4:-18633022 MS.gene044391:intron
TTGCAGCAGTCAAACGGTGG+AGG 0.600335 8.4:-18634750 MS.gene044391:intron
CCTCAAGATCTTGTGGAGGA+GGG 0.600496 8.4:+18633190 None:intergenic
GGAAGCTGCAAACTGGAGGT+GGG 0.610613 8.4:-18633321 MS.gene044391:CDS
ATTACGTTACACAGTGACAA+TGG 0.613795 8.4:-18633402 MS.gene044391:intron
GACATAAACTTTGGTTCACT+AGG 0.613858 8.4:+18633640 None:intergenic
CAATAGCAAATCAAATAACG+TGG 0.614557 8.4:-18633145 MS.gene044391:CDS
CATGTCACTACTTTGGGTCA+GGG 0.618397 8.4:-18633288 MS.gene044391:CDS
ACTTCCTCAAGATCTTGTGG+AGG 0.620137 8.4:+18633186 None:intergenic
AATAACGTAGGCTTTCCTGT+TGG 0.623309 8.4:+18635580 None:intergenic
TTGATTCATAGTTACCAGAA+GGG 0.636080 8.4:-18633750 MS.gene044391:intron
CAGAAATACAACAGAGACTG+AGG 0.637183 8.4:-18634585 MS.gene044391:intron
ATAGCTTGGGAGTGAAATGT+CGG 0.637360 8.4:+18633356 None:intergenic
TACTGGAAGCTGCAAACTGG+AGG 0.637452 8.4:-18633325 MS.gene044391:CDS
TGACCCAAAGTAGTGACATG+TGG 0.660177 8.4:+18633291 None:intergenic
ATATTGCAGCAGTCAAACGG+TGG 0.662282 8.4:-18634753 MS.gene044391:intron
GCTTCGAATAAGTCACATGT+CGG 0.667125 8.4:+18635680 None:intergenic
GTCACTACTTTGGGTCAGGG+AGG 0.667405 8.4:-18633285 MS.gene044391:CDS
ACAAATGGAAGACTCACTCA+GGG 0.670210 8.4:-18634038 MS.gene044391:CDS
GGAAAGCCTACGTTATTACA+GGG 0.679926 8.4:-18635574 MS.gene044391:CDS
GACATGTGACTTATTCGAAG+CGG 0.683369 8.4:-18635678 MS.gene044391:CDS
CCACTTAGAAGCCAAGACAA+AGG 0.694579 8.4:+18633685 None:intergenic
CGTTATTACAGGGAGAACGA+AGG 0.718082 8.4:-18635564 MS.gene044391:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATAGATAGAAAAAA+GGG + chr8.4:18634791-18634810 None:intergenic 10.0%
!! TATAATAATAGATAGAAAAA+AGG + chr8.4:18634792-18634811 None:intergenic 10.0%
!! ATATTTAATATCTCAGTTAT+TGG - chr8.4:18634493-18634512 MS.gene044391:intron 15.0%
!! TAAATTGAAGATAAAGAAAT+TGG - chr8.4:18632876-18632895 MS.gene044391:CDS 15.0%
!! TTATTCACAAAATATCAAAT+GGG - chr8.4:18633871-18633890 MS.gene044391:CDS 15.0%
!! TTTATTCACAAAATATCAAA+TGG - chr8.4:18633870-18633889 MS.gene044391:CDS 15.0%
!!! AGTATACCTATTTTTTTTTA+AGG + chr8.4:18633294-18633313 None:intergenic 15.0%
!!! AGTTTTCCTTAAAAAAAAAT+AGG - chr8.4:18633285-18633304 MS.gene044391:CDS 15.0%
!!! TATTGATATTTTGATTTCAT+CGG - chr8.4:18634695-18634714 MS.gene044391:intron 15.0%
!! AAAATACACTATTATTTACC+TGG + chr8.4:18634768-18634787 None:intergenic 20.0%
!! AAAGTAATATATGAATTACG+AGG + chr8.4:18633600-18633619 None:intergenic 20.0%
!! AATCTTCAAAATCATTTACA+AGG + chr8.4:18633800-18633819 None:intergenic 20.0%
!! CATAAATGTCAAATAATGTT+GGG + chr8.4:18634283-18634302 None:intergenic 20.0%
!! CGAAAAATTTAAATTGAACA+AGG + chr8.4:18634201-18634220 None:intergenic 20.0%
!! GTCAAATACTATCATAATTT+GGG - chr8.4:18634445-18634464 MS.gene044391:intron 20.0%
!! TCATAAATGTCAAATAATGT+TGG + chr8.4:18634284-18634303 None:intergenic 20.0%
!! TCTATACAAAAGGTAATATT+TGG - chr8.4:18634594-18634613 MS.gene044391:CDS 20.0%
!! TGATAGTATTTGACATAATT+TGG + chr8.4:18634439-18634458 None:intergenic 20.0%
!! TGTCAAATACTATCATAATT+TGG - chr8.4:18634444-18634463 MS.gene044391:intron 20.0%
!!! ACTTATCATTTTAATCTCAA+AGG - chr8.4:18633202-18633221 MS.gene044391:CDS 20.0%
!!! ATTTTATTTTTCCAGCAATA+TGG - chr8.4:18633401-18633420 MS.gene044391:intron 20.0%
!!! GATCATAATTGTTATTAACT+TGG + chr8.4:18635477-18635496 None:intergenic 20.0%
!!! TTATTTGATTTGCTATTGAT+TGG + chr8.4:18635445-18635464 None:intergenic 20.0%
!!! TTTGGTCTTGTTTAAAATTT+AGG - chr8.4:18633675-18633694 MS.gene044391:CDS 20.0%
! ATTTCCATTTAACTCATTCA+AGG + chr8.4:18635338-18635357 None:intergenic 25.0%
! CAATTGAACTCATAACAATT+TGG + chr8.4:18633937-18633956 None:intergenic 25.0%
! CCAAAATCACTATTTGAAAA+TGG - chr8.4:18635499-18635518 MS.gene044391:intron 25.0%
! CGAAGGTAATGTAATAATAA+TGG - chr8.4:18633045-18633064 MS.gene044391:CDS 25.0%
! CTTGTGATAAAATGAGAAAA+TGG - chr8.4:18633121-18633140 MS.gene044391:CDS 25.0%
! GAAAAAAACCTTCAAGAAAT+TGG - chr8.4:18633702-18633721 MS.gene044391:CDS 25.0%
! GTAAACAAGTTAGTATTTGT+TGG - chr8.4:18635628-18635647 MS.gene044391:CDS 25.0%
!! AGTTTGTACCAAAAAAGTAT+AGG + chr8.4:18634398-18634417 None:intergenic 25.0%
!! CCATTTTCAAATAGTGATTT+TGG + chr8.4:18635502-18635521 None:intergenic 25.0%
!! TTTCTCATTTTATCACAAGA+AGG + chr8.4:18633120-18633139 None:intergenic 25.0%
!! TTTGATGTAGTCTCAATTTT+CGG - chr8.4:18633638-18633657 MS.gene044391:CDS 25.0%
!!! ACTTTTACACACTTTAAAGA+TGG + chr8.4:18633332-18633351 None:intergenic 25.0%
!!! ATCATTTTAATCTCAAAGGA+CGG - chr8.4:18633206-18633225 MS.gene044391:CDS 25.0%
!!! TCCACAAACACATTTTTTTT+GGG + chr8.4:18634373-18634392 None:intergenic 25.0%
!!! TTCCACAAACACATTTTTTT+TGG + chr8.4:18634374-18634393 None:intergenic 25.0%
!!! TTTCTTTATTTTGCAGTAAC+TGG - chr8.4:18635659-18635678 MS.gene044391:CDS 25.0%
AACCAAAGTTTATGTCATTC+AGG - chr8.4:18634959-18634978 MS.gene044391:intron 30.0%
AAGCACATACAAAATTGGTA+AGG + chr8.4:18634128-18634147 None:intergenic 30.0%
ACCCAAAAAAAATGTGTTTG+TGG - chr8.4:18634369-18634388 MS.gene044391:intron 30.0%
AGAATCTTAAGCTGGAAATT+AGG - chr8.4:18633908-18633927 MS.gene044391:intron 30.0%
ATCATGATCCAATTTCTTGA+AGG + chr8.4:18633713-18633732 None:intergenic 30.0%
ATTGCTATGTATAACGAGAA+TGG - chr8.4:18635559-18635578 MS.gene044391:intron 30.0%
CAATAGCAAATCAAATAACG+TGG - chr8.4:18635447-18635466 MS.gene044391:intron 30.0%
CAATCGATTGTCTATACAAA+AGG - chr8.4:18634584-18634603 MS.gene044391:intron 30.0%
GTAAAGATACAAGATTTCCT+GGG - chr8.4:18633733-18633752 MS.gene044391:CDS 30.0%
GTTTACCACATAACTAGAAA+GGG + chr8.4:18635614-18635633 None:intergenic 30.0%
TACACACTCATAGTTAATAC+AGG + chr8.4:18634166-18634185 None:intergenic 30.0%
TCGGTCATTGCTTAAAAATT+TGG - chr8.4:18633657-18633676 MS.gene044391:CDS 30.0%
TCTGACTTCTACAATAAAGA+AGG - chr8.4:18635692-18635711 MS.gene044391:CDS 30.0%
TGCACATTTAAGTAAGGATT+TGG + chr8.4:18635361-18635380 None:intergenic 30.0%
TGTAAAGATACAAGATTTCC+TGG - chr8.4:18633732-18633751 MS.gene044391:CDS 30.0%
TGTTTACCACATAACTAGAA+AGG + chr8.4:18635615-18635634 None:intergenic 30.0%
TTAAAGTGTGTAAAAGTCGA+AGG - chr8.4:18633335-18633354 MS.gene044391:CDS 30.0%
TTGATTCATAGTTACCAGAA+GGG - chr8.4:18634842-18634861 MS.gene044391:intron 30.0%
TTGCAAAGCACATACAAAAT+TGG + chr8.4:18634133-18634152 None:intergenic 30.0%
! AAAGAGCTTTGTAACAAAAG+TGG - chr8.4:18635010-18635029 MS.gene044391:intron 30.0%
! AGGTAATATTTGGTTTCATG+CGG - chr8.4:18634604-18634623 MS.gene044391:CDS 30.0%
! ATCTTCACAACGATTTTCTA+AGG + chr8.4:18634670-18634689 None:intergenic 30.0%
! ATTGATTCATAGTTACCAGA+AGG - chr8.4:18634841-18634860 MS.gene044391:intron 30.0%
! TCTTCACAACGATTTTCTAA+GGG + chr8.4:18634669-18634688 None:intergenic 30.0%
! TTTTCTTGTTCTAATTGAGC+AGG - chr8.4:18633538-18633557 MS.gene044391:intron 30.0%
!! ATTGCTTTAGAATCTTAAGC+TGG - chr8.4:18633900-18633919 MS.gene044391:intron 30.0%
!! TTATTTTTCCAGCAATATGG+AGG - chr8.4:18633404-18633423 MS.gene044391:intron 30.0%
!! TTGATTTGCTATTGATTGGT+TGG + chr8.4:18635441-18635460 None:intergenic 30.0%
!!! ACAAACACATTTTTTTTGGG+TGG + chr8.4:18634370-18634389 None:intergenic 30.0%
!!! TGTGGAAACCTATACTTTTT+TGG - chr8.4:18634387-18634406 MS.gene044391:intron 30.0%
!!! TTTTTCCCTTTCTAGTTATG+TGG - chr8.4:18635606-18635625 MS.gene044391:CDS 30.0%
!!! TTTTTTGGGTGGTGTTTATT+TGG + chr8.4:18634359-18634378 None:intergenic 30.0%
AAAACTACATGCTTACCCTA+TGG + chr8.4:18635588-18635607 None:intergenic 35.0%
AAGAATCTCAACCTTTGTCT+TGG - chr8.4:18634896-18634915 MS.gene044391:CDS 35.0%
AAGTGAGAATCTTACAAGCT+CGG - chr8.4:18633980-18633999 MS.gene044391:intron 35.0%
AATTCTACAAATGGCTCTTG+TGG - chr8.4:18635535-18635554 MS.gene044391:intron 35.0%
ACTTTACCCTTATTGATGTC+TGG - chr8.4:18634868-18634887 MS.gene044391:CDS 35.0%
ATAACGAGAATGGTTACCAT+AGG - chr8.4:18635569-18635588 MS.gene044391:CDS 35.0%
ATTACGTTACACAGTGACAA+TGG - chr8.4:18635190-18635209 MS.gene044391:intron 35.0%
CACCTGAATGACATAAACTT+TGG + chr8.4:18634964-18634983 None:intergenic 35.0%
CACTGTTGCACATTTAAGTA+AGG + chr8.4:18635367-18635386 None:intergenic 35.0%
CAGTATAACCAGAATAGCTT+GGG + chr8.4:18635252-18635271 None:intergenic 35.0%
CATTTGTAGAATTCCAGAAC+TGG + chr8.4:18635528-18635547 None:intergenic 35.0%
CTTGGAACATCTTAGACAAA+TGG - chr8.4:18634539-18634558 MS.gene044391:intron 35.0%
GGTACCTTGAATGAGTTAAA+TGG - chr8.4:18635331-18635350 MS.gene044391:intron 35.0%
GTTCTAATTGAGCAGGAAAA+TGG - chr8.4:18633545-18633564 MS.gene044391:intron 35.0%
TAACGAGAATGGTTACCATA+GGG - chr8.4:18635570-18635589 MS.gene044391:CDS 35.0%
TCTCAGTTATTGGAGTAATC+TGG - chr8.4:18634503-18634522 MS.gene044391:intron 35.0%
TCTGGAGAAGATCAATAACT+TGG - chr8.4:18634521-18634540 MS.gene044391:intron 35.0%
TGTAGACCAGACATCAATAA+GGG + chr8.4:18634877-18634896 None:intergenic 35.0%
TTCATATTGCAGCAGTCAAA+CGG - chr8.4:18633836-18633855 MS.gene044391:intron 35.0%
TTGTAGACCAGACATCAATA+AGG + chr8.4:18634878-18634897 None:intergenic 35.0%
! AGATTCTCACTTGATGACTT+AGG + chr8.4:18633972-18633991 None:intergenic 35.0%
! CTTCACAACGATTTTCTAAG+GGG + chr8.4:18634668-18634687 None:intergenic 35.0%
! GACATAAACTTTGGTTCACT+AGG + chr8.4:18634955-18634974 None:intergenic 35.0%
!! CAGTTCTGGAATTCTACAAA+TGG - chr8.4:18635526-18635545 MS.gene044391:intron 35.0%
!! GTTTATTTGGTGTGAGCTAA+AGG + chr8.4:18634346-18634365 None:intergenic 35.0%
!!! GATATTTTGATTTCATCGGC+AGG - chr8.4:18634699-18634718 MS.gene044391:intron 35.0%
AATAACGTAGGCTTTCCTGT+TGG + chr8.4:18633015-18633034 None:intergenic 40.0%
AATGATCTCCTCCATATTGC+TGG + chr8.4:18633415-18633434 None:intergenic 40.0%
ACAAATGGAAGACTCACTCA+GGG - chr8.4:18634554-18634573 MS.gene044391:intron 40.0%
ACTTATTCGAAGCGGAAAAG+TGG - chr8.4:18632922-18632941 MS.gene044391:intron 40.0%
AGGAAAGCCTACGTTATTAC+AGG - chr8.4:18633017-18633036 MS.gene044391:intron 40.0%
ATAGCTTGGGAGTGAAATGT+CGG + chr8.4:18635239-18635258 None:intergenic 40.0%
ATTTCACTCCCAAGCTATTC+TGG - chr8.4:18635241-18635260 MS.gene044391:intron 40.0%
CAGAAATACAACAGAGACTG+AGG - chr8.4:18634007-18634026 MS.gene044391:intron 40.0%
CCAGTATAACCAGAATAGCT+TGG + chr8.4:18635253-18635272 None:intergenic 40.0%
CGCATTCAAGTGACATTAGT+TGG + chr8.4:18634741-18634760 None:intergenic 40.0%
CTGTTGGAGAAAACATGAGA+AGG + chr8.4:18632999-18633018 None:intergenic 40.0%
CTTATTCGAAGCGGAAAAGT+GGG - chr8.4:18632923-18632942 MS.gene044391:intron 40.0%
GACATGTGACTTATTCGAAG+CGG - chr8.4:18632914-18632933 MS.gene044391:intron 40.0%
GCTTCGAATAAGTCACATGT+CGG + chr8.4:18632915-18632934 None:intergenic 40.0%
GGAAAGCCTACGTTATTACA+GGG - chr8.4:18633018-18633037 MS.gene044391:intron 40.0%
GTTCACTAGGTAACATCAAC+TGG + chr8.4:18634942-18634961 None:intergenic 40.0%
TACAAGATTTCCTGGGTTCA+AGG - chr8.4:18633740-18633759 MS.gene044391:CDS 40.0%
TCTCCACATGTCACTACTTT+GGG - chr8.4:18635298-18635317 MS.gene044391:intron 40.0%
TTATACTGGAAGCTGCAAAC+TGG - chr8.4:18635264-18635283 MS.gene044391:intron 40.0%
TTCACTTCCTCAAGATCTTG+TGG + chr8.4:18635412-18635431 None:intergenic 40.0%
TTGAAAATGGACACCAGTTC+TGG - chr8.4:18635512-18635531 MS.gene044391:intron 40.0%
! AACCTTTTAGCCCTTTCTTG+TGG + chr8.4:18633459-18633478 None:intergenic 40.0%
! ACCTTTTAGCCCTTTCTTGT+GGG + chr8.4:18633458-18633477 None:intergenic 40.0%
! CCAAGCTATTCTGGTTATAC+TGG - chr8.4:18635250-18635269 MS.gene044391:intron 40.0%
! CTTCTCATGTTTTCTCCAAC+AGG - chr8.4:18632997-18633016 MS.gene044391:intron 40.0%
! TGAGTTCAATTGCCATTTGC+AGG - chr8.4:18633945-18633964 MS.gene044391:intron 40.0%
!! ACAACGATTTTCTAAGGGGA+CGG + chr8.4:18634664-18634683 None:intergenic 40.0%
AAGGGTAAAGTCATCCCTTC+TGG + chr8.4:18634859-18634878 None:intergenic 45.0%
ACATGTCACTACTTTGGGTC+AGG - chr8.4:18635303-18635322 MS.gene044391:intron 45.0%
ACTTCCTCAAGATCTTGTGG+AGG + chr8.4:18635409-18635428 None:intergenic 45.0%
ATATTGCAGCAGTCAAACGG+TGG - chr8.4:18633839-18633858 MS.gene044391:intron 45.0%
CAATCACTTACCTTGAACCC+AGG + chr8.4:18633753-18633772 None:intergenic 45.0%
CATGTCACTACTTTGGGTCA+GGG - chr8.4:18635304-18635323 MS.gene044391:intron 45.0%
CCAACTTAGCCCACAAGAAA+GGG - chr8.4:18633446-18633465 MS.gene044391:intron 45.0%
CCACTTAGAAGCCAAGACAA+AGG + chr8.4:18634910-18634929 None:intergenic 45.0%
CCTTTGTCTTGGCTTCTAAG+TGG - chr8.4:18634907-18634926 MS.gene044391:intron 45.0%
CGTTATTACAGGGAGAACGA+AGG - chr8.4:18633028-18633047 MS.gene044391:CDS 45.0%
CGTTCTCCCTGTAATAACGT+AGG + chr8.4:18633027-18633046 None:intergenic 45.0%
CTCTCCACATGTCACTACTT+TGG - chr8.4:18635297-18635316 MS.gene044391:intron 45.0%
GACAAATGGAAGACTCACTC+AGG - chr8.4:18634553-18634572 MS.gene044391:intron 45.0%
GATGACTTAGGTCCTGCAAA+TGG + chr8.4:18633960-18633979 None:intergenic 45.0%
GTCACTTGAATGCGTTAACC+AGG - chr8.4:18634747-18634766 MS.gene044391:intron 45.0%
TGACCCAAAGTAGTGACATG+TGG + chr8.4:18635304-18635323 None:intergenic 45.0%
! CCTTTCTTGTGGGCTAAGTT+GGG + chr8.4:18633448-18633467 None:intergenic 45.0%
! TTGGTGTGAGCTAAAGGCAT+GGG + chr8.4:18634340-18634359 None:intergenic 45.0%
! TTTGGTGTGAGCTAAAGGCA+TGG + chr8.4:18634341-18634360 None:intergenic 45.0%
!! CAACGATTTTCTAAGGGGAC+GGG + chr8.4:18634663-18634682 None:intergenic 45.0%
!!! AGGTGCAACTTTGGAGCTTT+TGG - chr8.4:18634027-18634046 MS.gene044391:CDS 45.0%
!! AAATAAAAATAAATATTTAG+TGG + chr8.4:18633494-18633513 None:intergenic 5.0%
!! AATAAAAATAAATATTTAGT+GGG + chr8.4:18633493-18633512 None:intergenic 5.0%
!!! TTCATATATTTTAAAAAAAA+TGG - chr8.4:18633231-18633250 MS.gene044391:CDS 5.0%
CAGAGACTGAGGTGCAACTT+TGG - chr8.4:18634018-18634037 MS.gene044391:CDS 50.0%
CCCAACTTAGCCCACAAGAA+AGG - chr8.4:18633445-18633464 MS.gene044391:intron 50.0%
CCTCAAGATCTTGTGGAGGA+GGG + chr8.4:18635405-18635424 None:intergenic 50.0%
GCCCACAAGAAAGGGCTAAA+AGG - chr8.4:18633454-18633473 MS.gene044391:intron 50.0%
TACTGGAAGCTGCAAACTGG+AGG - chr8.4:18635267-18635286 MS.gene044391:intron 50.0%
TCCTCAAGATCTTGTGGAGG+AGG + chr8.4:18635406-18635425 None:intergenic 50.0%
! CCCTTTCTTGTGGGCTAAGT+TGG + chr8.4:18633449-18633468 None:intergenic 50.0%
! TGGTGTGAGCTAAAGGCATG+GGG + chr8.4:18634339-18634358 None:intergenic 50.0%
ACTACTTTGGGTCAGGGAGG+TGG - chr8.4:18635310-18635329 MS.gene044391:intron 55.0%
CCCTCCTCCACAAGATCTTG+AGG - chr8.4:18635402-18635421 MS.gene044391:intron 55.0%
GGAAGCTGCAAACTGGAGGT+GGG - chr8.4:18635271-18635290 MS.gene044391:intron 55.0%
GTCACTACTTTGGGTCAGGG+AGG - chr8.4:18635307-18635326 MS.gene044391:intron 55.0%
TGGAAGCTGCAAACTGGAGG+TGG - chr8.4:18635270-18635289 MS.gene044391:intron 55.0%
TTGCAGCAGTCAAACGGTGG+AGG - chr8.4:18633842-18633861 MS.gene044391:intron 55.0%
! TTCTAAGGGGACGGGAACGA+TGG + chr8.4:18634655-18634674 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 18632865 18635749 18632865 ID=MS.gene044391
chr8.4 mRNA 18632865 18635749 18632865 ID=MS.gene044391.t1;Parent=MS.gene044391
chr8.4 exon 18635565 18635749 18635565 ID=MS.gene044391.t1.exon1;Parent=MS.gene044391.t1
chr8.4 CDS 18635565 18635749 18635565 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18635139 18635198 18635139 ID=MS.gene044391.t1.exon2;Parent=MS.gene044391.t1
chr8.4 CDS 18635139 18635198 18635139 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18635036 18635054 18635036 ID=MS.gene044391.t1.exon3;Parent=MS.gene044391.t1
chr8.4 CDS 18635036 18635054 18635036 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18634853 18634917 18634853 ID=MS.gene044391.t1.exon4;Parent=MS.gene044391.t1
chr8.4 CDS 18634853 18634917 18634853 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18634751 18634766 18634751 ID=MS.gene044391.t1.exon5;Parent=MS.gene044391.t1
chr8.4 CDS 18634751 18634766 18634751 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18634586 18634647 18634586 ID=MS.gene044391.t1.exon6;Parent=MS.gene044391.t1
chr8.4 CDS 18634586 18634647 18634586 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18634009 18634105 18634009 ID=MS.gene044391.t1.exon7;Parent=MS.gene044391.t1
chr8.4 CDS 18634009 18634105 18634009 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18633846 18633893 18633846 ID=MS.gene044391.t1.exon8;Parent=MS.gene044391.t1
chr8.4 CDS 18633846 18633893 18633846 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18633634 18633761 18633634 ID=MS.gene044391.t1.exon9;Parent=MS.gene044391.t1
chr8.4 CDS 18633634 18633761 18633634 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18633023 18633410 18633023 ID=MS.gene044391.t1.exon10;Parent=MS.gene044391.t1
chr8.4 CDS 18633023 18633410 18633023 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
chr8.4 exon 18632865 18632903 18632865 ID=MS.gene044391.t1.exon11;Parent=MS.gene044391.t1
chr8.4 CDS 18632865 18632903 18632865 ID=cds.MS.gene044391.t1;Parent=MS.gene044391.t1
Gene Sequence

>MS.gene044391

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGGTAATGTAATAATAATGGTGAAGCATTAATATGTGTCACATGTTTCTAGTACGACGAACAATTGTTTCCTTCTTGTGATAAAATGAGAAAATGGTACAATAATTGTTGAATGATTAACATGCGTCGTCGATTGATCAATATGTGAATCACACACTTATCATTTTAATCTCAAAGGACGGTGTTCATATATTTTAAAAAAAATGGTTGTAACATGAACTTTAGTGTCGAATAAGAGAGTTTTCCTTAAAAAAAAATAGGTATACTAGACTAATATAAAATCCATCTTTAAAGTGTGTAAAAGTCGAAGGAAATATCAAATATATGACAAGTTTTGAATGAACATGCATGTTGATTTTATTTTTCCAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGCCCACAAGAAAGGGCTAAAAGGTTAGTCAAATTTTCCCACTAAATATTTATTTTTATTTATTCATGATGAATGATCTAATATCTTTTCTTGTTCTAATTGAGCAGGAAAATGGAAAGTCTTGAAGTAAGATTACTTTTATCTCCTCGTAATTCATATATTACTTTTGTTGCATCGAGTGTGTATTTGATGTAGTCTCAATTTTCGGTCATTGCTTAAAAATTTGGTCTTGTTTAAAATTTAGGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGGTAAGTGATTGTGATTATCAAATTATCAATGTCTTCCTTGTAAATGATTTTGAAGATTCTGACATGTTTTAATTTTCATATTGCAGCAGTCAAACGGTGGAGGTACTGTTTATTCACAAAATATCAAATGGGTTAATAATTGCTTTAGAATCTTAAGCTGGAAATTAGGCGACCAAATTGTTATGAGTTCAATTGCCATTTGCAGGACCTAAGTCATCAAGTGAGAATCTTACAAGCTCGGCTCACAGAAATACAACAGAGACTGAGGTGCAACTTTGGAGCTTTTGGTTGTTAAAGTATTTCTTTTTCTTTTTTGTGTTATTTTATTGTTCTCTTGTTGAACTATTATTCATTCAGCTTCTACCTTACCAATTTTGTATGTGCTTTGCAATTGTTGTATACCTGTATTAACTATGAGTGTGTATGTACTTTTTTTCCTTGTTCAATTTAAATTTTTCGTATTCTTTTTTTCGTTATCATTCACATATCTTCATTCACATTTCATTAAAATTGTTTCACCCAACATTATTTGACATTTATGAGACTTATAAGCATTATAGACAAACTATTCTAACCCCATGCCTTTAGCTCACACCAAATAAACACCACCCAAAAAAAATGTGTTTGTGGAAACCTATACTTTTTTGGTACAAACTCTGTTGATTAAAACATCACCAAATTATGTCAAATACTATCATAATTTGGGCTATCATATAGTAATGTTGTTTTGAATATTTAATATCTCAGTTATTGGAGTAATCTGGAGAAGATCAATAACTTGGAACATCTTAGACAAATGGAAGACTCACTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGTAATATTTGGTTTCATGCGGACTAGTCATACAATTATATCATTTTCCATCGTTCCCGTCCCCTTAGAAAATCGTTGTGAAGATGTGTGTATTGATATTTTGATTTCATCGGCAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGGTAAATAATAGTGTATTTTCCCTTTTTTCTATCTATTATTATAATTTTTGAAATAATCTTATCTGAGATGATATTGATTCATAGTTACCAGAAGGGATGACTTTACCCTTATTGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGGTGAGTTAACAAGTTGCTTTCATAGACATAAAGAGCTTTGTAACAAAAGTGGATCGCATTTATACGAGAGTTATACATTTTAATGAACTTTCATATTTGCTTTTTAACTAATATGTTTCATAATTCTGCTATTTTTCAAATGTTCACTATGAAAATAAAATAAAATATATATATTGCTTATTTCATTTTATAATCTAGTTATTAATTTAATTACGTTACACAGTGACAATGGCAATAGAGATGTTGAGTGCTCAACCGACATTTCACTCCCAAGCTATTCTGGTTATACTGGAAGCTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTACTTTGGGTCAGGGAGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTTGAGGAAGTGAAACATCACCAACCAATCAATAGCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGGCTCTTGTGGTATTGCTATGTATAACGAGAATGGTTACCATAGGGTAAGCATGTAGTTTTTTTCCCTTTCTAGTTATGTGGTAAACAAGTTAGTATTTGTTGGTTTATTATTTTCTTTATTTTGCAGTAACTGGTTGATATATGTCTGACTTCTACAATAAAGAAGGATGATGATGACTTAAATGATGTGACAATTGCTTGA
Protein sequence

>MS.gene044391.t1

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSSQTVEDLSHQVRILQARLTEIQQRLSYWSNLEKINNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLLMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNGNRDVECSTDISLPSYSGYTGSCKLEVGSSPHVTTLGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINSKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNGSCGIAMYNENGYHRKDDDDLNDVTIA