AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044467

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044467.t1 MTR_4g109770 96.383 470 17 0 1 470 1 470 0.0 938
MS.gene044467.t1 MTR_2g461920 61.866 493 149 11 12 470 52 539 1.80e-171 493
MS.gene044467.t1 MTR_5g046670 49.315 219 88 8 56 264 174 379 1.15e-50 179
MS.gene044467.t1 MTR_3g099080 54.348 138 42 4 75 198 43 173 3.83e-38 143
MS.gene044467.t1 MTR_7g028740 35.331 317 179 8 75 382 26 325 8.64e-38 142
MS.gene044467.t1 MTR_8g096780 38.194 288 132 11 75 339 45 309 1.10e-36 139
MS.gene044467.t1 MTR_7g444860 70.000 80 24 0 117 196 143 222 6.29e-34 130
MS.gene044467.t1 MTR_7g092930 69.136 81 25 0 117 197 62 142 6.93e-34 130
MS.gene044467.t1 MTR_3g085180 50.769 130 57 2 72 198 115 240 8.23e-34 132
MS.gene044467.t1 MTR_1g053715 38.197 233 106 6 123 351 74 272 1.69e-33 129
MS.gene044467.t1 MTR_8g005960 73.684 76 20 0 123 198 187 262 4.83e-33 128
MS.gene044467.t1 MTR_4g088555 72.973 74 20 0 123 196 63 136 7.48e-33 122
MS.gene044467.t1 MTR_2g014200 70.886 79 23 0 118 196 59 137 3.18e-32 120
MS.gene044467.t1 MTR_8g463140 70.270 74 22 0 123 196 65 138 2.80e-31 119
MS.gene044467.t1 MTR_2g078770 70.270 74 22 0 123 196 58 131 1.17e-30 117
MS.gene044467.t1 MTR_7g110320 69.737 76 23 0 121 196 143 218 1.53e-30 125
MS.gene044467.t1 MTR_2g046550 45.946 148 69 3 58 196 67 212 2.32e-30 125
MS.gene044467.t1 MTR_7g110320 69.737 76 23 0 121 196 143 218 2.49e-30 125
MS.gene044467.t1 MTR_1g035010 62.366 93 31 1 110 198 87 179 1.53e-29 123
MS.gene044467.t1 MTR_1g086250 69.737 76 23 0 121 196 145 220 1.91e-29 122
MS.gene044467.t1 MTR_8g080680 36.607 224 103 8 122 339 131 321 2.56e-28 116
MS.gene044467.t1 MTR_8g080690 63.158 76 28 0 123 198 151 226 5.28e-28 115
MS.gene044467.t1 MTR_8g080670 61.644 73 28 0 123 195 162 234 4.09e-25 107
MS.gene044467.t1 MTR_2g020620 51.562 64 31 0 133 196 100 163 2.28e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044467.t1 AT1G69170 64.286 126 32 3 75 197 83 198 7.65e-45 162
MS.gene044467.t1 AT5G50670 46.067 178 84 4 123 288 101 278 2.53e-37 140
MS.gene044467.t1 AT5G50670 46.067 178 84 4 123 288 101 278 2.53e-37 140
MS.gene044467.t1 AT5G50570 46.067 178 84 4 123 288 101 278 2.53e-37 140
MS.gene044467.t1 AT5G50570 46.067 178 84 4 123 288 101 278 2.53e-37 140
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 2.32e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27370 68.966 87 27 0 123 209 176 262 5.48e-36 137
MS.gene044467.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.50e-35 135
MS.gene044467.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.50e-35 135
MS.gene044467.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.50e-35 135
MS.gene044467.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.50e-35 135
MS.gene044467.t1 AT5G43270 46.199 171 68 6 48 209 94 249 6.78e-34 132
MS.gene044467.t1 AT5G43270 46.199 171 68 6 48 209 94 249 6.78e-34 132
MS.gene044467.t1 AT5G43270 46.199 171 68 6 48 209 94 249 6.78e-34 132
MS.gene044467.t1 AT2G33810 67.089 79 26 0 118 196 49 127 6.63e-33 121
MS.gene044467.t1 AT3G57920 70.588 85 25 0 114 198 50 134 1.34e-32 127
MS.gene044467.t1 AT2G42200 75.000 76 19 0 123 198 74 149 1.75e-32 127
MS.gene044467.t1 AT1G02065 72.727 77 21 0 120 196 185 261 2.11e-32 126
MS.gene044467.t1 AT2G47070 73.684 76 20 0 121 196 104 179 2.57e-31 128
MS.gene044467.t1 AT3G60030 72.973 74 20 0 123 196 127 200 4.26e-31 127
MS.gene044467.t1 AT1G53160 51.667 120 48 3 123 232 54 173 4.77e-31 118
MS.gene044467.t1 AT1G53160 51.667 120 48 3 123 232 54 173 4.77e-31 118
MS.gene044467.t1 AT1G20980 62.000 100 35 1 110 206 104 203 8.42e-31 127
MS.gene044467.t1 AT1G76580 72.368 76 21 0 123 198 114 189 3.27e-30 124
MS.gene044467.t1 AT1G76580 72.368 76 21 0 123 198 114 189 3.31e-30 124
MS.gene044467.t1 AT1G53160 62.069 87 31 1 123 207 54 140 4.29e-30 115
MS.gene044467.t1 AT1G76580 72.368 76 21 0 123 198 114 189 6.36e-30 124
MS.gene044467.t1 AT3G15270 63.636 77 28 0 123 199 63 139 1.03e-28 112
MS.gene044467.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.16e-22 101
MS.gene044467.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.25e-22 101
MS.gene044467.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.38e-22 100
MS.gene044467.t1 AT2G47070 76.000 50 12 0 147 196 1 50 5.15e-18 87.4
MS.gene044467.t1 AT1G02065 64.706 51 18 0 120 170 185 235 2.50e-16 79.0

Find 96 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCATATTGTCAAGTTTA+TGG 0.148069 8.1:+15915580 MS.gene044467:CDS
AGGTCTTCCAAAGTGGTTTC+TGG 0.202370 8.1:+15917093 MS.gene044467:CDS
GGCGTTCACTCTACAAATTC+TGG 0.217076 8.1:+15916515 MS.gene044467:CDS
CTGCTGGTGGCTTGGTTCCC+TGG 0.251209 8.1:-15917052 None:intergenic
GGACTGGCTTGAAGTGTTAT+TGG 0.259748 8.1:-15917423 None:intergenic
CGAAATCACTTTAAGTAATT+CGG 0.269479 8.1:-15917802 None:intergenic
AGTTATATTTCTGAAGAAAA+AGG 0.280021 8.1:+15915235 MS.gene044467:CDS
CACAAACTTTATGCCTCTTA+TGG 0.303462 8.1:-15915636 None:intergenic
CAAAATAGGTGCTGCTCCTT+TGG 0.306496 8.1:-15915506 None:intergenic
GGGAGACTGTTTCAGCCATT+TGG 0.327396 8.1:+15916536 MS.gene044467:CDS
TATGGCCTGCAAGTCGTTTA+CGG 0.342741 8.1:-15916469 None:intergenic
TCAGATGATTTCAATTTCAT+TGG 0.348900 8.1:+15915301 MS.gene044467:CDS
CCAAGCATTCAAGGATTATC+AGG 0.351371 8.1:+15917359 MS.gene044467:CDS
GCTTCATGTTCTGCTTTCAT+AGG 0.354620 8.1:-15917119 None:intergenic
TCCATCTGTAGGATTTAATT+CGG 0.357577 8.1:-15917555 None:intergenic
TTATTGTCTGGTACCAATAT+AGG 0.364521 8.1:-15917590 None:intergenic
CGACAGTTGTAGTAAATTGA+TGG 0.372715 8.1:-15917705 None:intergenic
GTTCCAAGCAAGAAACTTGA+TGG 0.403736 8.1:+15915328 MS.gene044467:CDS
TGAGTGCACTCTCACTCTCT+TGG 0.404810 8.1:-15915554 None:intergenic
CATACCGCACCAAGCATTCA+AGG 0.405157 8.1:+15917350 MS.gene044467:CDS
CAAGGATCTTAGTTCTTGTA+AGG 0.406648 8.1:+15915609 MS.gene044467:CDS
TGATATCGTGAACTTTGAAA+TGG 0.410198 8.1:+15917613 MS.gene044467:CDS
GACTCACAAGAAGACAAAAT+AGG 0.410388 8.1:-15915520 None:intergenic
TCTTGCAGATATCAGATTCC+AGG 0.416127 8.1:+15917034 MS.gene044467:intron
AATTCCCTTTGATAACCCTT+TGG 0.423487 8.1:+15917451 MS.gene044467:CDS
CAGAACATGAAGCTGGCTTT+AGG 0.424799 8.1:+15917129 MS.gene044467:CDS
TGTTGCATGCAATGGAATCT+TGG 0.426426 8.1:+15915212 None:intergenic
GAGTGCACTCTCACTCTCTT+GGG 0.430597 8.1:-15915553 None:intergenic
TTACCATCAAGTTTCTTGCT+TGG 0.431934 8.1:-15915331 None:intergenic
AGTTGCCGTAAACGACTTGC+AGG 0.435658 8.1:+15916464 MS.gene044467:CDS
CCTGATAATCCTTGAATGCT+TGG 0.438418 8.1:-15917359 None:intergenic
AAGTCATCTCAGTCCTATAT+TGG 0.441537 8.1:+15917577 MS.gene044467:CDS
ATTCCATTCACAATGACTAT+AGG 0.443748 8.1:-15915676 None:intergenic
CAAAGGGAATTTCCAAGGAC+TGG 0.449402 8.1:-15917439 None:intergenic
TCTCTCATTGATTTGAAGCT+AGG 0.463051 8.1:+15915439 MS.gene044467:CDS
CACAATGACTATAGGAGTCT+TGG 0.465991 8.1:-15915668 None:intergenic
TAACACTTCAAGCCAGTCCT+TGG 0.468157 8.1:+15917427 MS.gene044467:CDS
CATTCACAAAATCTGAATCA+TGG 0.469185 8.1:-15917649 None:intergenic
TCTTCTGTGCCTGTCACCAA+TGG 0.476821 8.1:+15917158 MS.gene044467:CDS
AGCCAATGGCATGCGCATAA+TGG 0.479009 8.1:-15917292 None:intergenic
GCATCATAGACAATCATTGC+AGG 0.480777 8.1:+15917748 MS.gene044467:CDS
GCGTTCACTCTACAAATTCT+GGG 0.481125 8.1:+15916516 MS.gene044467:CDS
TTCACGATATCATTATTGTC+TGG 0.484373 8.1:-15917602 None:intergenic
AGCCATTATGCGCATGCCAT+TGG 0.493081 8.1:+15917290 MS.gene044467:CDS
CTAGGAAGATTTGATGATCA+TGG 0.493709 8.1:+15915457 MS.gene044467:CDS
GCCATTGGCTACACCTCCTT+AGG 0.499701 8.1:+15917305 MS.gene044467:CDS
ATTGAAGTCTTCGCTTCCTA+AGG 0.501723 8.1:-15917321 None:intergenic
TATATATTATTACCACCAAA+TGG 0.502996 8.1:-15916551 None:intergenic
AAAAGATGCTGCTGGTGGCT+TGG 0.511710 8.1:-15917060 None:intergenic
TAGACATGGCTATGGCTGCT+TGG 0.512721 8.1:-15917479 None:intergenic
TGTGGTGGAATCCAATGGTT+GGG 0.513935 8.1:+15915414 MS.gene044467:CDS
ACTCCTATAGTCATTGTGAA+TGG 0.516933 8.1:+15915673 MS.gene044467:CDS
TTGCTGTCTGAGTTTGATGA+TGG 0.518570 8.1:+15916434 MS.gene044467:CDS
TCCGAATTAAATCCTACAGA+TGG 0.523648 8.1:+15917554 MS.gene044467:CDS
AATGAGAGAATCCCAACCAT+TGG 0.523865 8.1:-15915425 None:intergenic
AAACCACTTTGGAAGACCTC+TGG 0.529651 8.1:-15917089 None:intergenic
TAGGTCTCCAGAAACCACTT+TGG 0.532795 8.1:-15917100 None:intergenic
CTTGTAAGGACTACCATAAG+AGG 0.534199 8.1:+15915623 MS.gene044467:CDS
TTTCAAGCTTTCAAATTCTG+TGG 0.537070 8.1:+15915396 MS.gene044467:CDS
TTGCATCATCTTCACAAGAA+AGG 0.541164 8.1:-15917682 None:intergenic
TTATGCTGTAGACATGGCTA+TGG 0.541912 8.1:-15917487 None:intergenic
AATGTTGCCACATCCACTAC+AGG 0.543311 8.1:+15917245 MS.gene044467:CDS
ATAACCAAAGGGTTATCAAA+GGG 0.544827 8.1:-15917455 None:intergenic
CAAGCTTTCAAATTCTGTGG+TGG 0.545205 8.1:+15915399 MS.gene044467:CDS
GTTATCAAAGGGAATTTCCA+AGG 0.547080 8.1:-15917444 None:intergenic
AGACTGTTTCAGCCATTTGG+TGG 0.551343 8.1:+15916539 MS.gene044467:CDS
GGATAGAGAAAAGATGCTGC+TGG 0.557359 8.1:-15917068 None:intergenic
AATAACCAAAGGGTTATCAA+AGG 0.561243 8.1:-15917456 None:intergenic
TGGCTGCTTGGAATAACCAA+AGG 0.562021 8.1:-15917467 None:intergenic
CTGTGGTGGAATCCAATGGT+TGG 0.564108 8.1:+15915413 MS.gene044467:CDS
ATGAAAGCAGAACATGAAGC+TGG 0.566946 8.1:+15917122 MS.gene044467:CDS
GGCTGCTTGGAATAACCAAA+GGG 0.567623 8.1:-15917466 None:intergenic
GAAGTCTTCGCTTCCTAAGG+AGG 0.569996 8.1:-15917318 None:intergenic
GTGAACTTTGAAATGGAAGA+CGG 0.572384 8.1:+15917620 MS.gene044467:CDS
CTTACTGCCTGTAGTGGATG+TGG 0.574967 8.1:-15917252 None:intergenic
TGAACTTTGAAATGGAAGAC+GGG 0.575100 8.1:+15917621 MS.gene044467:CDS
ACTGAACTTACTGCCTGTAG+TGG 0.586429 8.1:-15917258 None:intergenic
CTGAGATGACTTCCATCTGT+AGG 0.587562 8.1:-15917566 None:intergenic
TCTTGATTGAAGATGTCCAT+TGG 0.588804 8.1:-15917174 None:intergenic
TCTATCAGACACTCGGCTTG+AGG 0.591247 8.1:-15917525 None:intergenic
TGAAGATGTCCATTGGTGAC+AGG 0.591626 8.1:-15917167 None:intergenic
CTTGCAGATATCAGATTCCA+GGG 0.591867 8.1:+15917035 MS.gene044467:intron
TGCACTCTCACTCTCTTGGG+AGG 0.598164 8.1:-15915550 None:intergenic
TCCTAAGGAGGTGTAGCCAA+TGG 0.598734 8.1:-15917306 None:intergenic
TATCCAGAGGTCTTCCAAAG+TGG 0.601895 8.1:+15917086 MS.gene044467:CDS
TTGTGAATGGAATTGAGCAG+AGG 0.621516 8.1:+15915686 MS.gene044467:CDS
AATTCTGTGGTGGAATCCAA+TGG 0.627306 8.1:+15915409 MS.gene044467:CDS
TGGCAATGCTTCAATTGATG+CGG 0.627501 8.1:+15915477 MS.gene044467:CDS
TAAGAGGCATAAAGTTTGTG+AGG 0.628037 8.1:+15915639 MS.gene044467:CDS
GTGATCTTATGCTGTAGACA+TGG 0.639068 8.1:-15917493 None:intergenic
ATAAAAGTCTATCAGACACT+CGG 0.644463 8.1:-15917532 None:intergenic
TCAAGTTTATGGATGTAACA+AGG 0.656151 8.1:+15915591 MS.gene044467:CDS
TTTGTAGAGTGAACGCCTTG+TGG 0.665425 8.1:-15916509 None:intergenic
TAGAGAAAAGATGCTGCTGG+TGG 0.692709 8.1:-15917065 None:intergenic
TAATCCTTGAATGCTTGGTG+CGG 0.726498 8.1:-15917354 None:intergenic
GAGCGTCGAAGAAAACCACA+AGG 0.736819 8.1:+15916494 MS.gene044467:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAATTAATTTAGTAAAA+TGG - chr8.1:15915933-15915952 None:intergenic 10.0%
!! AAGAAATCAATGTAATTTAA+TGG - chr8.1:15916653-15916672 None:intergenic 15.0%
!! AGAAATCAATGTAATTTAAT+GGG - chr8.1:15916652-15916671 None:intergenic 15.0%
!! GGATACAATTATATAAAAAA+GGG - chr8.1:15915885-15915904 None:intergenic 15.0%
!! TGGATACAATTATATAAAAA+AGG - chr8.1:15915886-15915905 None:intergenic 15.0%
!!! TGTAAAATTGATTCTATATT+TGG + chr8.1:15915774-15915793 MS.gene044467:intron 15.0%
!! ACTTATTCAACAATAGTTAA+TGG + chr8.1:15916701-15916720 MS.gene044467:intron 20.0%
!! ATTGTTGAATAAGTAACTTA+TGG - chr8.1:15916695-15916714 None:intergenic 20.0%
!! GAAGTAATAAACTTGAAAAT+TGG - chr8.1:15915738-15915757 None:intergenic 20.0%
!! TAGTAACTTAGTTATATGAA+TGG - chr8.1:15916836-15916855 None:intergenic 20.0%
!! TATATATTATTACCACCAAA+TGG - chr8.1:15916554-15916573 None:intergenic 20.0%
!!! AAAATTGATTCTATATTTGG+TGG + chr8.1:15915777-15915796 MS.gene044467:intron 20.0%
!!! AAACTAATTTTGTTTAAGTC+TGG - chr8.1:15915988-15916007 None:intergenic 20.0%
!!! AGTTATATTTCTGAAGAAAA+AGG + chr8.1:15915235-15915254 MS.gene044467:CDS 20.0%
! AAAACATGTGATCACAATTT+AGG - chr8.1:15916206-15916225 None:intergenic 25.0%
! AGAAATTAAGTATCTCCTTA+TGG + chr8.1:15916731-15916750 MS.gene044467:intron 25.0%
! ATAGTTATGATATATAGGTC+TGG + chr8.1:15916380-15916399 MS.gene044467:intron 25.0%
! ATTGTAAATAATCACACATG+TGG + chr8.1:15916130-15916149 MS.gene044467:intron 25.0%
! CAAATGAATGTGAAAGAAAA+AGG - chr8.1:15916617-15916636 None:intergenic 25.0%
! GCTGTATAGTTATGATATAT+AGG + chr8.1:15916375-15916394 MS.gene044467:intron 25.0%
! TCAGATGATTTCAATTTCAT+TGG + chr8.1:15915301-15915320 MS.gene044467:CDS 25.0%
!! TAAGAAGCATAGTAATCAAT+AGG - chr8.1:15916298-15916317 None:intergenic 25.0%
!! TTTAGCATCACAATAGTTTT+TGG + chr8.1:15916005-15916024 MS.gene044467:intron 25.0%
!!! AAAATGGATTATGACTAAAG+AGG - chr8.1:15915917-15915936 None:intergenic 25.0%
!!! ATTTTGTTTAAGTCTGGTTT+TGG - chr8.1:15915982-15916001 None:intergenic 25.0%
!!! CATGTTTTTGTTTTAAACCT+TGG + chr8.1:15916218-15916237 MS.gene044467:intron 25.0%
!!! TAATGTTTAGCTTGTTATAC+AGG + chr8.1:15916405-15916424 MS.gene044467:intron 25.0%
!!! TACATTGATTTCTTTTGTTG+TGG + chr8.1:15916659-15916678 MS.gene044467:intron 25.0%
AATAACCAAAGGGTTATCAA+AGG - chr8.1:15917459-15917478 None:intergenic 30.0%
ACTGCATATTGTCAAGTTTA+TGG + chr8.1:15915580-15915599 MS.gene044467:CDS 30.0%
ATAAAAGTCTATCAGACACT+CGG - chr8.1:15917535-15917554 None:intergenic 30.0%
ATAACCAAAGGGTTATCAAA+GGG - chr8.1:15917458-15917477 None:intergenic 30.0%
ATAGTAATCAATAGGTGCAA+AGG - chr8.1:15916290-15916309 None:intergenic 30.0%
ATTCCATTCACAATGACTAT+AGG - chr8.1:15915679-15915698 None:intergenic 30.0%
ATTTCTAACTAGCTGGAATT+TGG + chr8.1:15915812-15915831 MS.gene044467:intron 30.0%
CATTCACAAAATCTGAATCA+TGG - chr8.1:15917652-15917671 None:intergenic 30.0%
CGAATGTTGAAAACAAAACA+AGG - chr8.1:15916259-15916278 None:intergenic 30.0%
GAAGTTTATGATTGACCATA+AGG - chr8.1:15916749-15916768 None:intergenic 30.0%
TAGTAATCAATAGGTGCAAA+GGG - chr8.1:15916289-15916308 None:intergenic 30.0%
TATGATTTGATACGATCCTA+TGG + chr8.1:15917010-15917029 MS.gene044467:intron 30.0%
TCAAGTTTATGGATGTAACA+AGG + chr8.1:15915591-15915610 MS.gene044467:CDS 30.0%
TCATGTTCTAGTATTCCAAT+AGG - chr8.1:15916039-15916058 None:intergenic 30.0%
TCCATCTGTAGGATTTAATT+CGG - chr8.1:15917558-15917577 None:intergenic 30.0%
TGAATGGAAACTCAGAATTT+AGG - chr8.1:15916820-15916839 None:intergenic 30.0%
TGACTAAAGAGGTTAATGTT+TGG - chr8.1:15915906-15915925 None:intergenic 30.0%
TTATTGTCTGGTACCAATAT+AGG - chr8.1:15917593-15917612 None:intergenic 30.0%
TTCACGATATCATTATTGTC+TGG - chr8.1:15917605-15917624 None:intergenic 30.0%
TTTCAAGCTTTCAAATTCTG+TGG + chr8.1:15915396-15915415 MS.gene044467:CDS 30.0%
! AAGTGAGTTTTCTGTAAAAG+GGG + chr8.1:15915366-15915385 MS.gene044467:CDS 30.0%
! ACTAAAAAAGCATAGGAAAC+TGG - chr8.1:15916969-15916988 None:intergenic 30.0%
! ACTATACAGCTTCTTATTTG+AGG - chr8.1:15916365-15916384 None:intergenic 30.0%
! ATTTTGCATTTCTAACTAGC+TGG + chr8.1:15915805-15915824 MS.gene044467:intron 30.0%
! CAAGTGAGTTTTCTGTAAAA+GGG + chr8.1:15915365-15915384 MS.gene044467:CDS 30.0%
! CTTTTACAGAAAACTCACTT+GGG - chr8.1:15915366-15915385 None:intergenic 30.0%
! GCAACATTTTCATGAAGAAA+GGG - chr8.1:15917233-15917252 None:intergenic 30.0%
! TAGGCTTTTGTGAAATGTTT+GGG + chr8.1:15915269-15915288 MS.gene044467:CDS 30.0%
! TCAGATGACTAAAAAAGCAT+AGG - chr8.1:15916976-15916995 None:intergenic 30.0%
! TGATATCGTGAACTTTGAAA+TGG + chr8.1:15917613-15917632 MS.gene044467:CDS 30.0%
! TTAGGCTTTTGTGAAATGTT+TGG + chr8.1:15915268-15915287 MS.gene044467:CDS 30.0%
! TTTTACATGGTACAACACTA+AGG - chr8.1:15916860-15916879 None:intergenic 30.0%
!! AATTGTTTTTCGTTGTACGA+AGG - chr8.1:15917209-15917228 None:intergenic 30.0%
!! GAAAAAGGTTCTGAAGAATT+AGG + chr8.1:15915250-15915269 MS.gene044467:CDS 30.0%
!!! ATGAGCAGGGTTTTAAATTA+TGG + chr8.1:15916082-15916101 MS.gene044467:intron 30.0%
!!! TTGTTTTAAACCTTGGTCTT+GGG + chr8.1:15916225-15916244 MS.gene044467:intron 30.0%
!!! TTTGTTTTAAACCTTGGTCT+TGG + chr8.1:15916224-15916243 MS.gene044467:intron 30.0%
AAGTCATCTCAGTCCTATAT+TGG + chr8.1:15917577-15917596 MS.gene044467:CDS 35.0%
ACTCCTATAGTCATTGTGAA+TGG + chr8.1:15915673-15915692 MS.gene044467:CDS 35.0%
AGGGTTGAGCAATAAATTAG+AGG - chr8.1:15915866-15915885 None:intergenic 35.0%
CACAAACTTTATGCCTCTTA+TGG - chr8.1:15915639-15915658 None:intergenic 35.0%
CGACAGTTGTAGTAAATTGA+TGG - chr8.1:15917708-15917727 None:intergenic 35.0%
CTAAGTAGAAACATGACTGA+AGG - chr8.1:15916333-15916352 None:intergenic 35.0%
GACTCACAAGAAGACAAAAT+AGG - chr8.1:15915523-15915542 None:intergenic 35.0%
GTTATCAAAGGGAATTTCCA+AGG - chr8.1:15917447-15917466 None:intergenic 35.0%
GTTGAATTATGTCATGAGCA+GGG + chr8.1:15916069-15916088 MS.gene044467:intron 35.0%
TCATTCTGTAAGAAAGCAGA+TGG - chr8.1:15916771-15916790 None:intergenic 35.0%
TCCGAATTAAATCCTACAGA+TGG + chr8.1:15917554-15917573 MS.gene044467:CDS 35.0%
TCTTGATTGAAGATGTCCAT+TGG - chr8.1:15917177-15917196 None:intergenic 35.0%
TGTTGAATTATGTCATGAGC+AGG + chr8.1:15916068-15916087 MS.gene044467:intron 35.0%
TTACCATCAAGTTTCTTGCT+TGG - chr8.1:15915334-15915353 None:intergenic 35.0%
TTGCATCATCTTCACAAGAA+AGG - chr8.1:15917685-15917704 None:intergenic 35.0%
! AATTCCCTTTGATAACCCTT+TGG + chr8.1:15917451-15917470 MS.gene044467:CDS 35.0%
! ATGTAATTTAATGGGTGCAG+AGG - chr8.1:15916644-15916663 None:intergenic 35.0%
! CAAGGATCTTAGTTCTTGTA+AGG + chr8.1:15915609-15915628 MS.gene044467:CDS 35.0%
! CCAAGTGAGTTTTCTGTAAA+AGG + chr8.1:15915364-15915383 MS.gene044467:CDS 35.0%
! CCTTTTACAGAAAACTCACT+TGG - chr8.1:15915367-15915386 None:intergenic 35.0%
! CTAGGAAGATTTGATGATCA+TGG + chr8.1:15915457-15915476 MS.gene044467:CDS 35.0%
! CTTCAGTCATGTTTCTACTT+AGG + chr8.1:15916331-15916350 MS.gene044467:intron 35.0%
! CTTTTTCCCATTTCTAACGA+GGG - chr8.1:15916906-15916925 None:intergenic 35.0%
! GGCAACATTTTCATGAAGAA+AGG - chr8.1:15917234-15917253 None:intergenic 35.0%
! TAAGAGGCATAAAGTTTGTG+AGG + chr8.1:15915639-15915658 MS.gene044467:CDS 35.0%
! TCTTTTTCCCATTTCTAACG+AGG - chr8.1:15916907-15916926 None:intergenic 35.0%
! TGTAATTTAATGGGTGCAGA+GGG - chr8.1:15916643-15916662 None:intergenic 35.0%
! TTTTCGTTGTACGAAGGAAA+CGG - chr8.1:15917203-15917222 None:intergenic 35.0%
!! GTGAACTTTGAAATGGAAGA+CGG + chr8.1:15917620-15917639 MS.gene044467:CDS 35.0%
!! TCTCTCATTGATTTGAAGCT+AGG + chr8.1:15915439-15915458 MS.gene044467:CDS 35.0%
!! TGAACTTTGAAATGGAAGAC+GGG + chr8.1:15917621-15917640 MS.gene044467:CDS 35.0%
AATGAGAGAATCCCAACCAT+TGG - chr8.1:15915428-15915447 None:intergenic 40.0%
ATGAAAGCAGAACATGAAGC+TGG + chr8.1:15917122-15917141 MS.gene044467:CDS 40.0%
ATTGAAGTCTTCGCTTCCTA+AGG - chr8.1:15917324-15917343 None:intergenic 40.0%
CAAGCTTTCAAATTCTGTGG+TGG + chr8.1:15915399-15915418 MS.gene044467:CDS 40.0%
CACAATGACTATAGGAGTCT+TGG - chr8.1:15915671-15915690 None:intergenic 40.0%
CCAAGCATTCAAGGATTATC+AGG + chr8.1:15917359-15917378 MS.gene044467:CDS 40.0%
CCTGATAATCCTTGAATGCT+TGG - chr8.1:15917362-15917381 None:intergenic 40.0%
CTGATATCTGCAAGAACCAT+AGG - chr8.1:15917029-15917048 None:intergenic 40.0%
CTTGCAGATATCAGATTCCA+GGG + chr8.1:15917035-15917054 MS.gene044467:intron 40.0%
CTTGTAAGGACTACCATAAG+AGG + chr8.1:15915623-15915642 MS.gene044467:CDS 40.0%
GCATCATAGACAATCATTGC+AGG + chr8.1:15917748-15917767 MS.gene044467:CDS 40.0%
GCGTTCACTCTACAAATTCT+GGG + chr8.1:15916516-15916535 MS.gene044467:CDS 40.0%
GCTTCATGTTCTGCTTTCAT+AGG - chr8.1:15917122-15917141 None:intergenic 40.0%
GTGATCTTATGCTGTAGACA+TGG - chr8.1:15917496-15917515 None:intergenic 40.0%
GTTCCAAGCAAGAAACTTGA+TGG + chr8.1:15915328-15915347 MS.gene044467:CDS 40.0%
TCTTGCAGATATCAGATTCC+AGG + chr8.1:15917034-15917053 MS.gene044467:intron 40.0%
TGGCAATGCTTCAATTGATG+CGG + chr8.1:15915477-15915496 MS.gene044467:CDS 40.0%
TTATGCTGTAGACATGGCTA+TGG - chr8.1:15917490-15917509 None:intergenic 40.0%
TTCTGTCCCTCGTTAGAAAT+GGG + chr8.1:15916897-15916916 MS.gene044467:intron 40.0%
TTGCTGTCTGAGTTTGATGA+TGG + chr8.1:15916434-15916453 MS.gene044467:CDS 40.0%
TTGTCAACAATGTAGCAGGT+TGG + chr8.1:15915711-15915730 MS.gene044467:intron 40.0%
TTGTGAATGGAATTGAGCAG+AGG + chr8.1:15915686-15915705 MS.gene044467:CDS 40.0%
TTTCTGTCCCTCGTTAGAAA+TGG + chr8.1:15916896-15916915 MS.gene044467:intron 40.0%
! AATTCTGTGGTGGAATCCAA+TGG + chr8.1:15915409-15915428 MS.gene044467:CDS 40.0%
! AGCATCTTTTCTCTATCCAG+AGG + chr8.1:15917073-15917092 MS.gene044467:CDS 40.0%
! ATTGATGCGGCATTTTCCAA+AGG + chr8.1:15915490-15915509 MS.gene044467:CDS 40.0%
! GTTTTCTTCGACGCTCATTA+TGG - chr8.1:15916490-15916509 None:intergenic 40.0%
! TAATCCTTGAATGCTTGGTG+CGG - chr8.1:15917357-15917376 None:intergenic 40.0%
!! GGTTTTGTCAACAATGTAGC+AGG + chr8.1:15915707-15915726 MS.gene044467:CDS 40.0%
!! TTTTTGGTGTGTGTGCCTAT+TGG + chr8.1:15916021-15916040 MS.gene044467:intron 40.0%
AATGTTGCCACATCCACTAC+AGG + chr8.1:15917245-15917264 MS.gene044467:CDS 45.0%
ACTGAACTTACTGCCTGTAG+TGG - chr8.1:15917261-15917280 None:intergenic 45.0%
AGACTGTTTCAGCCATTTGG+TGG + chr8.1:15916539-15916558 MS.gene044467:CDS 45.0%
AGGTCTTCCAAAGTGGTTTC+TGG + chr8.1:15917093-15917112 MS.gene044467:CDS 45.0%
CAAAGGGAATTTCCAAGGAC+TGG - chr8.1:15917442-15917461 None:intergenic 45.0%
CATGTAAAATGCCGCTGACA+CGG + chr8.1:15916871-15916890 MS.gene044467:intron 45.0%
CTGAGATGACTTCCATCTGT+AGG - chr8.1:15917569-15917588 None:intergenic 45.0%
GGATAGAGAAAAGATGCTGC+TGG - chr8.1:15917071-15917090 None:intergenic 45.0%
GGCGTTCACTCTACAAATTC+TGG + chr8.1:15916515-15916534 MS.gene044467:CDS 45.0%
GGCTGCTTGGAATAACCAAA+GGG - chr8.1:15917469-15917488 None:intergenic 45.0%
TAACACTTCAAGCCAGTCCT+TGG + chr8.1:15917427-15917446 MS.gene044467:CDS 45.0%
TAGAGAAAAGATGCTGCTGG+TGG - chr8.1:15917068-15917087 None:intergenic 45.0%
TAGGTCTCCAGAAACCACTT+TGG - chr8.1:15917103-15917122 None:intergenic 45.0%
TATCCAGAGGTCTTCCAAAG+TGG + chr8.1:15917086-15917105 MS.gene044467:CDS 45.0%
TATGGCCTGCAAGTCGTTTA+CGG - chr8.1:15916472-15916491 None:intergenic 45.0%
TGGCTGCTTGGAATAACCAA+AGG - chr8.1:15917470-15917489 None:intergenic 45.0%
TTTGTAGAGTGAACGCCTTG+TGG - chr8.1:15916512-15916531 None:intergenic 45.0%
! AAACCACTTTGGAAGACCTC+TGG - chr8.1:15917092-15917111 None:intergenic 45.0%
! TGAAGATGTCCATTGGTGAC+AGG - chr8.1:15917170-15917189 None:intergenic 45.0%
! TGTGGTGGAATCCAATGGTT+GGG + chr8.1:15915414-15915433 MS.gene044467:CDS 45.0%
!! CAAAATAGGTGCTGCTCCTT+TGG - chr8.1:15915509-15915528 None:intergenic 45.0%
!! CAGAACATGAAGCTGGCTTT+AGG + chr8.1:15917129-15917148 MS.gene044467:CDS 45.0%
!! GGACTGGCTTGAAGTGTTAT+TGG - chr8.1:15917426-15917445 None:intergenic 45.0%
AAAAGATGCTGCTGGTGGCT+TGG - chr8.1:15917063-15917082 None:intergenic 50.0%
AGCCAATGGCATGCGCATAA+TGG - chr8.1:15917295-15917314 None:intergenic 50.0%
AGCCATTATGCGCATGCCAT+TGG + chr8.1:15917290-15917309 MS.gene044467:CDS 50.0%
AGTTGCCGTAAACGACTTGC+AGG + chr8.1:15916464-15916483 MS.gene044467:CDS 50.0%
CATACCGCACCAAGCATTCA+AGG + chr8.1:15917350-15917369 MS.gene044467:CDS 50.0%
GAAGTCTTCGCTTCCTAAGG+AGG - chr8.1:15917321-15917340 None:intergenic 50.0%
GAGTGCACTCTCACTCTCTT+GGG - chr8.1:15915556-15915575 None:intergenic 50.0%
GGACAGAAAATCCGTGTCAG+CGG - chr8.1:15916885-15916904 None:intergenic 50.0%
GGCTTCAAAACCCAAGACCA+AGG - chr8.1:15916238-15916257 None:intergenic 50.0%
GGGAGACTGTTTCAGCCATT+TGG + chr8.1:15916536-15916555 MS.gene044467:CDS 50.0%
TAGACATGGCTATGGCTGCT+TGG - chr8.1:15917482-15917501 None:intergenic 50.0%
TCTTCTGTGCCTGTCACCAA+TGG + chr8.1:15917158-15917177 MS.gene044467:CDS 50.0%
TGAGTGCACTCTCACTCTCT+TGG - chr8.1:15915557-15915576 None:intergenic 50.0%
! CGTGTCAGCGGCATTTTACA+TGG - chr8.1:15916873-15916892 None:intergenic 50.0%
! CTGTGGTGGAATCCAATGGT+TGG + chr8.1:15915413-15915432 MS.gene044467:CDS 50.0%
! TCCTAAGGAGGTGTAGCCAA+TGG - chr8.1:15917309-15917328 None:intergenic 50.0%
! TCTATCAGACACTCGGCTTG+AGG - chr8.1:15917528-15917547 None:intergenic 50.0%
!! CTTACTGCCTGTAGTGGATG+TGG - chr8.1:15917255-15917274 None:intergenic 50.0%
!! GAGCGTCGAAGAAAACCACA+AGG + chr8.1:15916494-15916513 MS.gene044467:CDS 50.0%
TGCACTCTCACTCTCTTGGG+AGG - chr8.1:15915553-15915572 None:intergenic 55.0%
! GCCATTGGCTACACCTCCTT+AGG + chr8.1:15917305-15917324 MS.gene044467:CDS 55.0%
!! CTGCTGGTGGCTTGGTTCCC+TGG - chr8.1:15917055-15917074 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 15915223 15917814 15915223 ID=MS.gene044467
chr8.1 mRNA 15915223 15917814 15915223 ID=MS.gene044467.t1;Parent=MS.gene044467
chr8.1 exon 15915223 15915728 15915223 ID=MS.gene044467.t1.exon1;Parent=MS.gene044467.t1
chr8.1 CDS 15915223 15915728 15915223 ID=cds.MS.gene044467.t1;Parent=MS.gene044467.t1
chr8.1 exon 15916427 15916560 15916427 ID=MS.gene044467.t1.exon2;Parent=MS.gene044467.t1
chr8.1 CDS 15916427 15916560 15916427 ID=cds.MS.gene044467.t1;Parent=MS.gene044467.t1
chr8.1 exon 15917042 15917814 15917042 ID=MS.gene044467.t1.exon3;Parent=MS.gene044467.t1
chr8.1 CDS 15917042 15917814 15917042 ID=cds.MS.gene044467.t1;Parent=MS.gene044467.t1
Gene Sequence

>MS.gene044467

ATGGAATCTTGGAGTTATATTTCTGAAGAAAAAGGTTCTGAAGAATTAGGCTTTTGTGAAATGTTTGGGAAACAATATTCAGATGATTTCAATTTCATTGGAAGTGTTCCAAGCAAGAAACTTGATGGTAAATTTGATGACCCAAGTGAGTTTTCTGTAAAAGGGGACACAAATTTCAAGCTTTCAAATTCTGTGGTGGAATCCAATGGTTGGGATTCTCTCATTGATTTGAAGCTAGGAAGATTTGATGATCATGGCAATGCTTCAATTGATGCGGCATTTTCCAAAGGAGCAGCACCTATTTTGTCTTCTTGTGAGTCTTCAACACCTCCCAAGAGAGTGAGAGTGCACTCAATGACTGCATATTGTCAAGTTTATGGATGTAACAAGGATCTTAGTTCTTGTAAGGACTACCATAAGAGGCATAAAGTTTGTGAGGTTCACTCCAAGACTCCTATAGTCATTGTGAATGGAATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTGGTCCAATTTTCAAGTTTATTACTTCTTTCAATTCATTTTAGTGTAAAATTGATTCTATATTTGGTGGAATTGATTTTGCATTTCTAACTAGCTGGAATTTGGAATTGTTGCTTCTACAATTGATTTTTAGCCTCTAATTTATTGCTCAACCCTTTTTTATATAATTGTATCCAAACATTAACCTCTTTAGTCATAATCCATTTTACTAAATTAATTCATTCAAAATTAATTTTTGTTATTGCAGAACCAAAACCAGACTTAAACAAAATTAGTTTAGCATCACAATAGTTTTTGGTGTGTGTGCCTATTGGAATACTAGAACATGAATCTTTACTTGTTGAATTATGTCATGAGCAGGGTTTTAAATTATGGTTGTGTTGCAATAATTGAAATTAATATTGTAAATAATCACACATGTGGCTTATTTGATTTTAATTATCACTGCGAAATACATCGAAAACTTTAATGTGCCTAAATTGTGATCACATGTTTTTGTTTTAAACCTTGGTCTTGGGTTTTGAAGCCTTGTTTTGTTTTCAACATTCGTTGCTTACCCTTTGCACCTATTGATTACTATGCTTCTTAAATGAAGTTTTTCCTTCAGTCATGTTTCTACTTAGGATGATAAGCCTCAAATAAGAAGCTGTATAGTTATGATATATAGGTCTGGTGTAATGTTTAGCTTGTTATACAGGTTTCATTTGCTGTCTGAGTTTGATGATGGTAAGCGCAGTTGCCGTAAACGACTTGCAGGCCATAATGAGCGTCGAAGAAAACCACAAGGCGTTCACTCTACAAATTCTGGGAGACTGTTTCAGCCATTTGGTGGTAATAATATATATAATAAAGTTAGATCATGATTTAACTTTATTCTTTACACTCCTTTTTCTTTCACATTCATTTGTTGCCCTCTGCACCCATTAAATTACATTGATTTCTTTTGTTGTGGTTGATGTGAACCATAAGTTACTTATTCAACAATAGTTAATGGTTGAAGCAGAAATTAAGTATCTCCTTATGGTCAATCATAAACTTCCATCTGCTTTCTTACAGAATGACATCTAAATCTAATTAATTGTAGTTACCTAAATTCTGAGTTTCCATTCATATAACTAAGTTACTAACCTTAGTGTTGTACCATGTAAAATGCCGCTGACACGGATTTTCTGTCCCTCGTTAGAAATGGGAAAAAGATGAAGCATTGTGACTCTTATAATAATTGATTATCTGAAGCCAGTTTCCTATGCTTTTTTAGTCATCTGATATTAATATAAATGTATGATTTGATACGATCCTATGGTTCTTGCAGATATCAGATTCCAGGGAACCAAGCCACCAGCAGCATCTTTTCTCTATCCAGAGGTCTTCCAAAGTGGTTTCTGGAGACCTATGAAAGCAGAACATGAAGCTGGCTTTAGGCATTTTTCTTCTGTGCCTGTCACCAATGGACATCTTCAATCAAGATCTCCGTTTCCTTCGTACAACGAAAAACAATTTCCCTTTCTTCATGAAAATGTTGCCACATCCACTACAGGCAGTAAGTTCAGTGAAAACAATAGCCATTATGCGCATGCCATTGGCTACACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTCATACCGCACCAAGCATTCAAGGATTATCAGGAATTTCAGACTCTTGTGCTCTCTCTCTTCTGTCATCTCAGTCCAATAACACTTCAAGCCAGTCCTTGGAAATTCCCTTTGATAACCCTTTGGTTATTCCAAGCAGCCATAGCCATGTCTACAGCATAAGATCACAAGCGACTTCCTCAAGCCGAGTGTCTGATAGACTTTTATCCGAATTAAATCCTACAGATGGAAGTCATCTCAGTCCTATATTGGTACCAGACAATAATGATATCGTGAACTTTGAAATGGAAGACGGGATCTTCCATGATTCAGATTTTGTGAATGCGAAAGACTGCCTTTCTTGTGAAGATGATGCAACCATCAATTTACTACAACTGTCGTCGCAGCTTCAACGAGTTGAGCATCATAGACAATCATTGCAGGTGAAGAAAGAGTTTGATTCTTCTTGCACTCTCCGAATTACTTAA
Protein sequence

>MS.gene044467.t1

MESWSYISEEKGSEELGFCEMFGKQYSDDFNFIGSVPSKKLDGKFDDPSEFSVKGDTNFKLSNSVVESNGWDSLIDLKLGRFDDHGNASIDAAFSKGAAPILSSCESSTPPKRVRVHSMTAYCQVYGCNKDLSSCKDYHKRHKVCEVHSKTPIVIVNGIEQRFCQQCSRFHLLSEFDDGKRSCRKRLAGHNERRRKPQGVHSTNSGRLFQPFGDIRFQGTKPPAASFLYPEVFQSGFWRPMKAEHEAGFRHFSSVPVTNGHLQSRSPFPSYNEKQFPFLHENVATSTTGSKFSENNSHYAHAIGYTSLGSEDFNVFHTAPSIQGLSGISDSCALSLLSSQSNNTSSQSLEIPFDNPLVIPSSHSHVYSIRSQATSSSRVSDRLLSELNPTDGSHLSPILVPDNNDIVNFEMEDGIFHDSDFVNAKDCLSCEDDATINLLQLSSQLQRVEHHRQSLQVKKEFDSSCTLRIT