Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04457.t1 | KEH15586.1 | 87.2 | 78 | 10 | 0 | 70 | 147 | 50 | 127 | 1.00E-26 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04457.t1 | Q6X7J4 | 48.7 | 115 | 27 | 4 | 1 | 90 | 1 | 108 | 1.3e-17 | 90.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04457.t1 | A0A072TEA9 | 87.2 | 78 | 10 | 0 | 70 | 147 | 50 | 127 | 7.5e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04457.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04457.t1 | MTR_7g025010 | 58.750 | 160 | 43 | 3 | 1 | 146 | 1 | 151 | 1.09e-45 | 157 |
| MS.gene04457.t1 | MTR_4g088080 | 56.115 | 139 | 33 | 5 | 1 | 124 | 1 | 126 | 2.68e-36 | 127 |
| MS.gene04457.t1 | MTR_0777s0020 | 87.179 | 78 | 10 | 0 | 70 | 147 | 50 | 127 | 3.93e-36 | 121 |
| MS.gene04457.t1 | MTR_4g088070 | 71.429 | 84 | 21 | 2 | 43 | 124 | 35 | 117 | 1.62e-30 | 114 |
| MS.gene04457.t1 | MTR_2g015000 | 52.500 | 120 | 31 | 4 | 1 | 98 | 1 | 116 | 9.09e-29 | 109 |
| MS.gene04457.t1 | MTR_4g088065 | 52.679 | 112 | 48 | 2 | 30 | 141 | 120 | 226 | 1.02e-27 | 103 |
| MS.gene04457.t1 | MTR_6g027390 | 67.391 | 46 | 15 | 0 | 43 | 88 | 38 | 83 | 1.50e-17 | 77.4 |
| MS.gene04457.t1 | MTR_7g086940 | 65.909 | 44 | 15 | 0 | 43 | 86 | 34 | 77 | 3.26e-15 | 70.9 |
| MS.gene04457.t1 | MTR_4g084550 | 43.636 | 55 | 31 | 0 | 44 | 98 | 71 | 125 | 2.62e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04457.t1 | AT2G33880 | 50.000 | 126 | 33 | 5 | 1 | 102 | 1 | 120 | 1.65e-27 | 105 |
| MS.gene04457.t1 | AT2G33880 | 50.000 | 126 | 33 | 5 | 1 | 102 | 1 | 120 | 2.19e-27 | 105 |
| MS.gene04457.t1 | AT5G45980 | 43.333 | 120 | 44 | 3 | 1 | 99 | 1 | 117 | 1.35e-22 | 91.3 |
| MS.gene04457.t1 | AT5G17810 | 65.217 | 46 | 16 | 0 | 43 | 88 | 27 | 72 | 1.99e-16 | 73.9 |
| MS.gene04457.t1 | AT5G17810 | 65.217 | 46 | 16 | 0 | 43 | 88 | 27 | 72 | 2.19e-16 | 73.9 |
| MS.gene04457.t1 | AT5G17810 | 65.217 | 46 | 16 | 0 | 43 | 88 | 27 | 72 | 2.19e-16 | 73.9 |
| MS.gene04457.t1 | AT3G03660 | 63.043 | 46 | 17 | 0 | 43 | 88 | 39 | 84 | 3.13e-16 | 73.6 |
| MS.gene04457.t1 | AT3G03660 | 63.043 | 46 | 17 | 0 | 43 | 88 | 39 | 84 | 4.67e-16 | 73.2 |
| MS.gene04457.t1 | AT3G03660 | 63.043 | 46 | 17 | 0 | 43 | 88 | 39 | 84 | 9.70e-16 | 72.4 |
| MS.gene04457.t1 | AT3G03660 | 63.043 | 46 | 17 | 0 | 43 | 88 | 39 | 84 | 1.25e-15 | 72.4 |
| MS.gene04457.t1 | AT2G17950 | 42.500 | 80 | 43 | 2 | 43 | 121 | 44 | 121 | 4.52e-13 | 65.1 |
Find 32 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCATTTGGTTGTGTTTC+TGG | 0.090039 | 3.4:+58164764 | None:intergenic |
| CGTGAACAATTACAAGAATT+TGG | 0.113481 | 3.4:-58164875 | MS.gene04457:CDS |
| TGTTTCTGGTCTCTTGTTGT+AGG | 0.261301 | 3.4:+58164778 | None:intergenic |
| GAAAGATGATGAAGATGATT+TGG | 0.341139 | 3.4:+58164703 | None:intergenic |
| ATTTGTGTGAGAGAGTTATT+AGG | 0.365608 | 3.4:+58164740 | None:intergenic |
| GTGAACAATTACAAGAATTT+GGG | 0.368349 | 3.4:-58164874 | MS.gene04457:CDS |
| CAGTTACGAGACAGGAGTTT+AGG | 0.401996 | 3.4:+58165575 | None:intergenic |
| CTAAACTCCTGTCTCGTAAC+TGG | 0.406804 | 3.4:-58165574 | MS.gene04457:intron |
| GTCGACTTCTTAAACATGTT+AGG | 0.407359 | 3.4:+58165640 | None:intergenic |
| TTCTTAATCTCATCTCTTGG+TGG | 0.415394 | 3.4:+58164902 | None:intergenic |
| GTTTCTGGTCTCTTGTTGTA+GGG | 0.424258 | 3.4:+58164779 | None:intergenic |
| GGCTATGTTGCTGATTCTGA+TGG | 0.431149 | 3.4:+58165596 | None:intergenic |
| ATCTTCTTAATCTCATCTCT+TGG | 0.440501 | 3.4:+58164899 | None:intergenic |
| CTGATTCTGATGGGTGGAGT+TGG | 0.440680 | 3.4:+58165606 | None:intergenic |
| TTAGGACAATGTTTGTTTGA+GGG | 0.465380 | 3.4:+58165658 | None:intergenic |
| TTACAAGAATTTGGGCAACT+TGG | 0.466911 | 3.4:-58164866 | MS.gene04457:CDS |
| GTTAGGACAATGTTTGTTTG+AGG | 0.473408 | 3.4:+58165657 | None:intergenic |
| GAGTTATTAGGAGTTGCATT+TGG | 0.477754 | 3.4:+58164752 | None:intergenic |
| ATGACATATTATGATTTCAC+CGG | 0.480595 | 3.4:+58164658 | None:intergenic |
| TATTATGATTTCACCGGGAG+AGG | 0.484380 | 3.4:+58164664 | None:intergenic |
| TTGAAAACCAGTTACGAGAC+AGG | 0.489558 | 3.4:+58165567 | None:intergenic |
| TTCTGATGGGTGGAGTTGGA+TGG | 0.503756 | 3.4:+58165610 | None:intergenic |
| GATGATGAAGATGATTTGGA+AGG | 0.542768 | 3.4:+58164707 | None:intergenic |
| GCTATGTTGCTGATTCTGAT+GGG | 0.545053 | 3.4:+58165597 | None:intergenic |
| CACCATCTCTGAGTTGAAGA+GGG | 0.555445 | 3.4:+58164928 | None:intergenic |
| TCACCATCTCTGAGTTGAAG+AGG | 0.563521 | 3.4:+58164927 | None:intergenic |
| TTTCTGGTCTCTTGTTGTAG+GGG | 0.566911 | 3.4:+58164780 | None:intergenic |
| ATGTTGCTGATTCTGATGGG+TGG | 0.582610 | 3.4:+58165600 | None:intergenic |
| GATGAAGATGATTTGGAAGG+AGG | 0.596007 | 3.4:+58164710 | None:intergenic |
| TTTGTCTGCTTATGGACTTG+CGG | 0.603126 | 3.4:+58164813 | None:intergenic |
| AGCCCTCTTCAACTCAGAGA+TGG | 0.607398 | 3.4:-58164930 | MS.gene04457:CDS |
| TGACATATTATGATTTCACC+GGG | 0.615673 | 3.4:+58164659 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCTTATAACAAATATTATTT+AGG | - | chr3.4:58165264-58165283 | MS.gene04457:intron | 15.0% |
| !! | TATAACAAATATTATTTAGG+TGG | - | chr3.4:58165267-58165286 | MS.gene04457:intron | 15.0% |
| !!! | TTTATTTTATACTATATCTG+TGG | - | chr3.4:58165073-58165092 | MS.gene04457:intron | 15.0% |
| !! | GCTTATAACAAATATTGTTT+AGG | - | chr3.4:58165333-58165352 | MS.gene04457:intron | 20.0% |
| !! | TATAACAAATATTGTTTAGG+TGG | - | chr3.4:58165336-58165355 | MS.gene04457:intron | 20.0% |
| !!! | ATCGATATAATTGTTTTGAT+TGG | + | chr3.4:58164915-58164934 | None:intergenic | 20.0% |
| !!! | TCAAAACAATTATATCGATT+TGG | - | chr3.4:58164916-58164935 | MS.gene04457:CDS | 20.0% |
| !!! | TTTCTTTATTCTAGTTTGAT+TGG | - | chr3.4:58165311-58165330 | MS.gene04457:intron | 20.0% |
| ! | ATGACATATTATGATTTCAC+CGG | + | chr3.4:58165664-58165683 | None:intergenic | 25.0% |
| ! | GTGAACAATTACAAGAATTT+GGG | - | chr3.4:58165445-58165464 | MS.gene04457:intron | 25.0% |
| !! | ATCATCATAATGATAATGAG+TGG | + | chr3.4:58164788-58164807 | None:intergenic | 25.0% |
| !! | CAAACAAATTTTATCTCTAC+AGG | - | chr3.4:58164974-58164993 | MS.gene04457:intron | 25.0% |
| !! | CTGTAGAGATAAAATTTGTT+TGG | + | chr3.4:58164976-58164995 | None:intergenic | 25.0% |
| ATCTTCTTAATCTCATCTCT+TGG | + | chr3.4:58165423-58165442 | None:intergenic | 30.0% | |
| ATTTGTGTGAGAGAGTTATT+AGG | + | chr3.4:58165582-58165601 | None:intergenic | 30.0% | |
| CGTGAACAATTACAAGAATT+TGG | - | chr3.4:58165444-58165463 | MS.gene04457:intron | 30.0% | |
| GAAAGATGATGAAGATGATT+TGG | + | chr3.4:58165619-58165638 | None:intergenic | 30.0% | |
| TGACATATTATGATTTCACC+GGG | + | chr3.4:58165663-58165682 | None:intergenic | 30.0% | |
| TTAGGACAATGTTTGTTTGA+GGG | + | chr3.4:58164664-58164683 | None:intergenic | 30.0% | |
| ! | AGTTTGTAGAGTCATTGATT+TGG | + | chr3.4:58164885-58164904 | None:intergenic | 30.0% |
| ! | TCATAATGATAATGAGTGGA+AGG | + | chr3.4:58164784-58164803 | None:intergenic | 30.0% |
| ! | TCTTTTCTTTTGTCTGCTTA+TGG | + | chr3.4:58165517-58165536 | None:intergenic | 30.0% |
| ! | TTTCGTTACTCTTGTTTGAT+TGG | - | chr3.4:58165242-58165261 | MS.gene04457:intron | 30.0% |
| !!! | ATTTGGTTTTTTCCATGTTG+GGG | - | chr3.4:58164933-58164952 | MS.gene04457:CDS | 30.0% |
| !!! | GATTTGGTTTTTTCCATGTT+GGG | - | chr3.4:58164932-58164951 | MS.gene04457:CDS | 30.0% |
| GAGTTATTAGGAGTTGCATT+TGG | + | chr3.4:58165570-58165589 | None:intergenic | 35.0% | |
| GTTAGGACAATGTTTGTTTG+AGG | + | chr3.4:58164665-58164684 | None:intergenic | 35.0% | |
| TTACAAGAATTTGGGCAACT+TGG | - | chr3.4:58165453-58165472 | MS.gene04457:intron | 35.0% | |
| TTCTTAATCTCATCTCTTGG+TGG | + | chr3.4:58165420-58165439 | None:intergenic | 35.0% | |
| ! | GATGATGAAGATGATTTGGA+AGG | + | chr3.4:58165615-58165634 | None:intergenic | 35.0% |
| ! | GTCGACTTCTTAAACATGTT+AGG | + | chr3.4:58164682-58164701 | None:intergenic | 35.0% |
| !!! | CGATTTGGTTTTTTCCATGT+TGG | - | chr3.4:58164931-58164950 | MS.gene04457:CDS | 35.0% |
| GTTGCATTTGGTTGTGTTTC+TGG | + | chr3.4:58165558-58165577 | None:intergenic | 40.0% | |
| TATTATGATTTCACCGGGAG+AGG | + | chr3.4:58165658-58165677 | None:intergenic | 40.0% | |
| TTCGTTATATGACCCCAACA+TGG | + | chr3.4:58164948-58164967 | None:intergenic | 40.0% | |
| TTGAAAACCAGTTACGAGAC+AGG | + | chr3.4:58164755-58164774 | None:intergenic | 40.0% | |
| TTTAGGTGGAGATGCTGAAT+TGG | - | chr3.4:58165281-58165300 | MS.gene04457:intron | 40.0% | |
| TTTGTCTGCTTATGGACTTG+CGG | + | chr3.4:58165509-58165528 | None:intergenic | 40.0% | |
| ! | GATGAAGATGATTTGGAAGG+AGG | + | chr3.4:58165612-58165631 | None:intergenic | 40.0% |
| ! | GTGATGCTAGTGTCTTTTAC+TGG | - | chr3.4:58165475-58165494 | MS.gene04457:intron | 40.0% |
| ! | TTTCTGGTCTCTTGTTGTAG+GGG | + | chr3.4:58165542-58165561 | None:intergenic | 40.0% |
| !! | GCTATGTTGCTGATTCTGAT+GGG | + | chr3.4:58164725-58164744 | None:intergenic | 40.0% |
| !! | GTTTCTGGTCTCTTGTTGTA+GGG | + | chr3.4:58165543-58165562 | None:intergenic | 40.0% |
| !! | TGTTTCTGGTCTCTTGTTGT+AGG | + | chr3.4:58165544-58165563 | None:intergenic | 40.0% |
| CAGTTACGAGACAGGAGTTT+AGG | + | chr3.4:58164747-58164766 | None:intergenic | 45.0% | |
| CTAAACTCCTGTCTCGTAAC+TGG | - | chr3.4:58164745-58164764 | MS.gene04457:CDS | 45.0% | |
| GATCATCAAAAAGCCTCTCC+CGG | - | chr3.4:58165642-58165661 | MS.gene04457:CDS | 45.0% | |
| ! | CACCATCTCTGAGTTGAAGA+GGG | + | chr3.4:58165394-58165413 | None:intergenic | 45.0% |
| ! | TCACCATCTCTGAGTTGAAG+AGG | + | chr3.4:58165395-58165414 | None:intergenic | 45.0% |
| !! | ATGTTGCTGATTCTGATGGG+TGG | + | chr3.4:58164722-58164741 | None:intergenic | 45.0% |
| !! | GGCTATGTTGCTGATTCTGA+TGG | + | chr3.4:58164726-58164745 | None:intergenic | 45.0% |
| AGCCCTCTTCAACTCAGAGA+TGG | - | chr3.4:58165389-58165408 | MS.gene04457:intron | 50.0% | |
| ! | CTGATTCTGATGGGTGGAGT+TGG | + | chr3.4:58164716-58164735 | None:intergenic | 50.0% |
| ! | TTCTGATGGGTGGAGTTGGA+TGG | + | chr3.4:58164712-58164731 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 58164655 | 58165686 | 58164655 | ID=MS.gene04457 |
| chr3.4 | mRNA | 58164655 | 58165686 | 58164655 | ID=MS.gene04457.t1;Parent=MS.gene04457 |
| chr3.4 | exon | 58165575 | 58165686 | 58165575 | ID=MS.gene04457.t1.exon1;Parent=MS.gene04457.t1 |
| chr3.4 | CDS | 58165575 | 58165686 | 58165575 | ID=cds.MS.gene04457.t1;Parent=MS.gene04457.t1 |
| chr3.4 | exon | 58164655 | 58164986 | 58164655 | ID=MS.gene04457.t1.exon2;Parent=MS.gene04457.t1 |
| chr3.4 | CDS | 58164655 | 58164986 | 58164655 | ID=cds.MS.gene04457.t1;Parent=MS.gene04457.t1 |
| Gene Sequence |
| Protein sequence |
