AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044732


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044732.t1 MTR_4g051538 89.362 235 24 1 1 235 1 234 1.87e-143 400
MS.gene044732.t1 MTR_3g104630 51.969 127 49 3 106 220 16 142 5.34e-31 112
MS.gene044732.t1 MTR_1g105920 31.098 164 96 4 1 150 1 161 1.51e-16 76.3
MS.gene044732.t1 MTR_1g012570 27.979 193 118 5 1 182 1 183 1.54e-16 76.3
MS.gene044732.t1 MTR_5g053390 28.194 227 112 8 1 205 1 198 2.87e-16 75.5
MS.gene044732.t1 MTR_3g080940 44.706 85 46 1 1 85 1 84 2.93e-16 75.1
MS.gene044732.t1 MTR_8g086290 30.818 159 96 3 4 151 7 162 3.52e-16 73.9
MS.gene044732.t1 MTR_1g108510 29.114 158 94 3 1 143 1 155 7.18e-16 74.3
MS.gene044732.t1 MTR_3g052920 33.333 147 88 4 1 137 1 147 8.90e-16 74.3
MS.gene044732.t1 MTR_1g106070 31.707 164 101 5 1 153 1 164 9.06e-16 73.2
MS.gene044732.t1 MTR_1g105910 32.609 138 78 3 1 126 1 135 1.10e-15 73.9
MS.gene044732.t1 MTR_3g030770 29.677 155 97 4 1 143 1 155 1.55e-15 73.6
MS.gene044732.t1 MTR_1g077360 40.244 82 49 0 1 82 1 82 1.80e-15 73.6
MS.gene044732.t1 MTR_2g030740 33.333 135 81 2 1 126 1 135 2.39e-15 73.2
MS.gene044732.t1 MTR_8g043650 31.852 135 83 3 1 126 1 135 2.39e-15 73.2
MS.gene044732.t1 MTR_3g052870 40.244 82 49 0 1 82 1 82 4.44e-15 72.0
MS.gene044732.t1 MTR_1g108580 43.056 72 41 0 1 72 1 72 5.30e-15 72.0
MS.gene044732.t1 MTR_2g105290 29.747 158 99 4 4 151 7 162 1.33e-14 69.7
MS.gene044732.t1 MTR_2g085250 38.961 77 47 0 1 77 1 77 1.34e-14 70.9
MS.gene044732.t1 MTR_2g085280 38.961 77 47 0 1 77 1 77 1.42e-14 70.9
MS.gene044732.t1 MTR_5g055100 30.833 120 65 2 1 120 1 102 1.74e-14 70.9
MS.gene044732.t1 MTR_5g045560 28.188 149 93 3 1 137 1 147 3.28e-14 70.1
MS.gene044732.t1 MTR_1g075600 36.585 82 52 0 1 82 1 82 1.64e-13 68.2
MS.gene044732.t1 MTR_4g094638 39.130 69 42 0 9 77 2 70 2.06e-13 66.6
MS.gene044732.t1 MTR_0121s0100 35.065 77 50 0 1 77 1 77 2.34e-13 65.5
MS.gene044732.t1 MTR_2g093190 37.662 77 48 0 1 77 1 77 3.00e-13 67.0
MS.gene044732.t1 MTR_4g094632 36.145 83 53 0 9 91 2 84 5.55e-13 65.1
MS.gene044732.t1 MTR_3g030780 32.979 94 61 1 1 92 1 94 7.56e-13 63.9
MS.gene044732.t1 MTR_0121s0080 25.904 166 106 4 1 152 1 163 5.42e-12 63.9
MS.gene044732.t1 MTR_8g046350 36.111 72 46 0 9 80 2 73 1.49e-11 61.2
MS.gene044732.t1 MTR_8g079502 29.412 136 73 4 9 123 2 135 3.27e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044732.t1 AT4G36590 50.000 68 34 0 5 72 3 70 1.73e-17 79.3
MS.gene044732.t1 AT1G29962 46.250 80 42 1 1 80 1 79 1.56e-16 75.1
MS.gene044732.t1 AT2G24840 30.488 164 95 3 7 154 61 221 4.58e-16 75.5
MS.gene044732.t1 AT2G34440 34.884 129 76 4 9 130 2 129 3.87e-15 71.2
MS.gene044732.t1 AT1G28460 45.455 77 41 1 1 77 1 76 5.29e-14 68.6
MS.gene044732.t1 AT5G60440 28.293 205 119 6 5 200 3 188 5.51e-14 70.1
MS.gene044732.t1 AT1G01530 27.419 124 78 2 5 116 3 126 3.85e-12 64.3
MS.gene044732.t1 AT3G04100 38.095 84 49 1 2 85 7 87 6.61e-12 63.2
MS.gene044732.t1 AT1G28450 41.250 80 46 1 1 80 1 79 9.60e-12 62.4
MS.gene044732.t1 AT1G65360 43.333 60 34 0 9 68 7 66 1.01e-11 62.8

Find 43 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGAGTTAACAACACTTTC+AGG 0.165743 4.4:-51351204 MS.gene044732:CDS
TTGAAGAAGTTGGAAAGTTC+TGG 0.270990 4.4:-51350973 MS.gene044732:CDS
TGCACCAACTTCATGATCTT+TGG 0.281401 4.4:+51350642 None:intergenic
ACACAAGGAGTCAACCTTAT+TGG 0.301989 4.4:-51350259 MS.gene044732:CDS
GATGGGTTTCCATAACAAAA+TGG 0.311638 4.4:+51351141 None:intergenic
ATAACCAATGTCTTGAAAAC+TGG 0.328346 4.4:-51350871 MS.gene044732:CDS
CTTATTGGTGTAAGCGGAAT+AGG 0.333563 4.4:-51350244 MS.gene044732:CDS
CTTCTACCATGTCATATATC+TGG 0.335672 4.4:+51350193 None:intergenic
CTATTCCGCTTACACCAATA+AGG 0.352863 4.4:+51350245 None:intergenic
TTTCTTTCACTTGATCATAT+TGG 0.353442 4.4:+51350929 None:intergenic
ATCATTTCCACTTCCATTGA+AGG 0.371552 4.4:+51351078 None:intergenic
AGTTGGAAAGTTCTGGATAT+TGG 0.382288 4.4:-51350966 MS.gene044732:CDS
ATTCTCATGCTTTCTCCAAC+GGG 0.403336 4.4:-51351168 MS.gene044732:CDS
TTTATTTACGACAGCAACTA+AGG 0.422183 4.4:+51350897 None:intergenic
TTTGTTATAGATTCAGAAGA+TGG 0.427015 4.4:+51351123 None:intergenic
CCGAGTTAACAACACTTTCA+GGG 0.450892 4.4:-51351203 MS.gene044732:CDS
CCCTGAAAGTGTTGTTAACT+CGG 0.457016 4.4:+51351203 None:intergenic
CGTAGCAATTTCTACCATTG+AGG 0.467584 4.4:-51350713 MS.gene044732:intron
GAAGGATGAAAATCCTTCAA+TGG 0.468568 4.4:-51351091 MS.gene044732:CDS
GACTCCATTCAGTTCCTCAA+TGG 0.472764 4.4:+51350699 None:intergenic
GGAAGTGGAAATGATGAAAC+CGG 0.474804 4.4:-51351070 MS.gene044732:CDS
GGTGTAAGCGGAATAGGATA+TGG 0.475102 4.4:-51350238 MS.gene044732:CDS
TGTTGCATTTACAACGAGAA+AGG 0.475609 4.4:-51351247 MS.gene044732:CDS
TGAAAATCCTTCAATGGAAG+TGG 0.475677 4.4:-51351085 MS.gene044732:CDS
TATTCTCATGCTTTCTCCAA+CGG 0.502164 4.4:-51351169 MS.gene044732:CDS
TTGTTATAGATTCAGAAGAT+GGG 0.510327 4.4:+51351124 None:intergenic
TCTACCATTGAGGAACTGAA+TGG 0.512244 4.4:-51350703 MS.gene044732:CDS
TTGTCCTCAAAGACAAAAGA+AGG 0.512656 4.4:+51350667 None:intergenic
GCATTTACAACGAGAAAGGC+TGG 0.523590 4.4:-51351243 MS.gene044732:CDS
TTATCCAAAAGGTAAAGGTA+TGG 0.531949 4.4:-51350317 MS.gene044732:intron
GCGCCGAAGAGGTCATCAAA+AGG 0.555723 4.4:-51351318 MS.gene044732:CDS
AACACCAAAGATCATGAAGT+TGG 0.566706 4.4:-51350646 MS.gene044732:CDS
TGGATATTGGTCGATAAAAG+AGG 0.572226 4.4:-51350953 MS.gene044732:CDS
GTCAACCTTATTGGTGTAAG+CGG 0.584588 4.4:-51350250 MS.gene044732:CDS
CGAGTTAACAACACTTTCAG+GGG 0.590811 4.4:-51351202 MS.gene044732:CDS
ACCAATGTCTTGAAAACTGG+AGG 0.591573 4.4:-51350868 MS.gene044732:CDS
AGCGGAATAGGATATGGAAG+TGG 0.601533 4.4:-51350232 MS.gene044732:CDS
GAGTAAGAATGGCGCCGAAG+AGG 0.609055 4.4:-51351329 None:intergenic
GGAGGAGAGATTGACCCTCA+TGG 0.634950 4.4:-51350850 MS.gene044732:intron
AAGATGTACACATACCATGA+GGG 0.689701 4.4:+51350836 None:intergenic
TGAAAGTGTTGTTAACTCGG+AGG 0.701093 4.4:+51351206 None:intergenic
AAGCATGAGAATATCAACTG+AGG 0.718686 4.4:+51351179 None:intergenic
AAAGGCTGGAATTTACAACA+AGG 0.729615 4.4:-51351229 MS.gene044732:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTGAATCAATGAA+AGG + chr4.4:51351189-51351208 None:intergenic 15.0%
!! CAATACATAATATATATGTT+AGG + chr4.4:51351161-51351180 None:intergenic 15.0%
!! CTTAAAATAATTAGGTAAAA+AGG + chr4.4:51350772-51350791 None:intergenic 15.0%
!!! TTCAATTTTTTTTATCCAAA+AGG - chr4.4:51351197-51351216 MS.gene044732:CDS 15.0%
!! CAGATTCACTTAAAATAATT+AGG + chr4.4:51350780-51350799 None:intergenic 20.0%
!! GTTGAAAAAAATTGAGAAAA+AGG - chr4.4:51350245-51350264 MS.gene044732:CDS 20.0%
!!! CTAATTATTTTAAGTGAATC+TGG - chr4.4:51350778-51350797 MS.gene044732:intron 20.0%
!!! TATAACAAAAACTAGTTTGA+AGG - chr4.4:51350416-51350435 MS.gene044732:intron 20.0%
!!! TGAAAAAAATGATGATCTTT+TGG - chr4.4:51350494-51350513 MS.gene044732:intron 20.0%
!!! TTTTTTTATCCAAAAGGTAA+AGG - chr4.4:51351203-51351222 MS.gene044732:CDS 20.0%
! ACGTAAAAATTTGAAGAAGT+TGG - chr4.4:51350542-51350561 MS.gene044732:intron 25.0%
! ATAGCTACAAAAAAAACACA+AGG - chr4.4:51351251-51351270 MS.gene044732:CDS 25.0%
! TCTTCTTAATTTCCATTACT+AGG - chr4.4:51351098-51351117 MS.gene044732:CDS 25.0%
! TTGTTATAGATTCAGAAGAT+GGG + chr4.4:51350404-51350423 None:intergenic 25.0%
! TTTCTTTCACTTGATCATAT+TGG + chr4.4:51350599-51350618 None:intergenic 25.0%
!! TTTGTTATAGATTCAGAAGA+TGG + chr4.4:51350405-51350424 None:intergenic 25.0%
!! TTTTTCATGAAATATCCTAG+CGG - chr4.4:51350945-51350964 MS.gene044732:CDS 25.0%
AATGACGTCAAATGATTTCA+TGG - chr4.4:51350743-51350762 MS.gene044732:intron 30.0%
ATAACCAATGTCTTGAAAAC+TGG - chr4.4:51350654-51350673 MS.gene044732:CDS 30.0%
CTAGAGATATTTGATGTGTT+GGG + chr4.4:51351026-51351045 None:intergenic 30.0%
TCTAGAGATATTTGATGTGT+TGG + chr4.4:51351027-51351046 None:intergenic 30.0%
TTATCCAAAAGGTAAAGGTA+TGG - chr4.4:51351208-51351227 MS.gene044732:CDS 30.0%
TTTATTTACGACAGCAACTA+AGG + chr4.4:51350631-51350650 None:intergenic 30.0%
! AAATCCTTCTTTTGTCTTTG+AGG - chr4.4:51350854-51350873 MS.gene044732:CDS 30.0%
! TCATTTTGCTCTATAATCTC+CGG + chr4.4:51350477-51350496 None:intergenic 30.0%
! TTTGGATAAAAAGCATGTTG+CGG - chr4.4:51350512-51350531 MS.gene044732:intron 30.0%
! TTTTGTAGCTATATCTTGAG+AGG + chr4.4:51351243-51351262 None:intergenic 30.0%
!! ACCATTTAAGTTGCACTTAT+GGG - chr4.4:51351057-51351076 MS.gene044732:CDS 30.0%
!! TACCATTTAAGTTGCACTTA+TGG - chr4.4:51351056-51351075 MS.gene044732:CDS 30.0%
AAAGGCTGGAATTTACAACA+AGG - chr4.4:51350296-51350315 MS.gene044732:CDS 35.0%
AACACCAAAGATCATGAAGT+TGG - chr4.4:51350879-51350898 MS.gene044732:CDS 35.0%
AAGATGTACACATACCATGA+GGG + chr4.4:51350692-51350711 None:intergenic 35.0%
AAGCATGAGAATATCAACTG+AGG + chr4.4:51350349-51350368 None:intergenic 35.0%
AATGTTTGCATGCCTAGTAA+TGG + chr4.4:51351113-51351132 None:intergenic 35.0%
ATCATTTCCACTTCCATTGA+AGG + chr4.4:51350450-51350469 None:intergenic 35.0%
GAAGGATGAAAATCCTTCAA+TGG - chr4.4:51350434-51350453 MS.gene044732:intron 35.0%
TAGACAAGATCAGATGTTGT+TGG - chr4.4:51350990-51351009 MS.gene044732:CDS 35.0%
TATTCTCATGCTTTCTCCAA+CGG - chr4.4:51350356-51350375 MS.gene044732:intron 35.0%
TCCCATAAGTGCAACTTAAA+TGG + chr4.4:51351061-51351080 None:intergenic 35.0%
TGAAAATCCTTCAATGGAAG+TGG - chr4.4:51350440-51350459 MS.gene044732:intron 35.0%
TGGATATTGGTCGATAAAAG+AGG - chr4.4:51350572-51350591 MS.gene044732:intron 35.0%
TGTTGCATTTACAACGAGAA+AGG - chr4.4:51350278-51350297 MS.gene044732:CDS 35.0%
TTGAAGAAGTTGGAAAGTTC+TGG - chr4.4:51350552-51350571 MS.gene044732:intron 35.0%
TTGTCCTCAAAGACAAAAGA+AGG + chr4.4:51350861-51350880 None:intergenic 35.0%
! ACGGGAAAACCATTTTGTTA+TGG - chr4.4:51350375-51350394 MS.gene044732:intron 35.0%
! GATGGGTTTCCATAACAAAA+TGG + chr4.4:51350387-51350406 None:intergenic 35.0%
! TAACAAAATGGTTTTCCCGT+TGG + chr4.4:51350375-51350394 None:intergenic 35.0%
!! AGTTGGAAAGTTCTGGATAT+TGG - chr4.4:51350559-51350578 MS.gene044732:intron 35.0%
!! TTTCCTTTTGATGACCTCTT+CGG + chr4.4:51350213-51350232 None:intergenic 35.0%
ACACAAGGAGTCAACCTTAT+TGG - chr4.4:51351266-51351285 MS.gene044732:CDS 40.0%
ACCAATGTCTTGAAAACTGG+AGG - chr4.4:51350657-51350676 MS.gene044732:CDS 40.0%
ATTCTCATGCTTTCTCCAAC+GGG - chr4.4:51350357-51350376 MS.gene044732:intron 40.0%
CAAGATGTACACATACCATG+AGG + chr4.4:51350693-51350712 None:intergenic 40.0%
CCGAGTTAACAACACTTTCA+GGG - chr4.4:51350322-51350341 MS.gene044732:intron 40.0%
CGAGTTAACAACACTTTCAG+GGG - chr4.4:51350323-51350342 MS.gene044732:intron 40.0%
CGTAGCAATTTCTACCATTG+AGG - chr4.4:51350812-51350831 MS.gene044732:intron 40.0%
CTATTCCGCTTACACCAATA+AGG + chr4.4:51351283-51351302 None:intergenic 40.0%
CTTATTGGTGTAAGCGGAAT+AGG - chr4.4:51351281-51351300 MS.gene044732:CDS 40.0%
GGAAGTGGAAATGATGAAAC+CGG - chr4.4:51350455-51350474 MS.gene044732:intron 40.0%
GTCAACCTTATTGGTGTAAG+CGG - chr4.4:51351275-51351294 MS.gene044732:CDS 40.0%
TCCGAGTTAACAACACTTTC+AGG - chr4.4:51350321-51350340 MS.gene044732:intron 40.0%
TCTACCATTGAGGAACTGAA+TGG - chr4.4:51350822-51350841 MS.gene044732:intron 40.0%
TGCACCAACTTCATGATCTT+TGG + chr4.4:51350886-51350905 None:intergenic 40.0%
! GCAACCATACCTTTACCTTT+TGG + chr4.4:51351215-51351234 None:intergenic 40.0%
!! CCCTGAAAGTGTTGTTAACT+CGG + chr4.4:51350325-51350344 None:intergenic 40.0%
!! TCCTCCAGTTTTCAAGACAT+TGG + chr4.4:51350661-51350680 None:intergenic 40.0%
!! TGAAAGTGTTGTTAACTCGG+AGG + chr4.4:51350322-51350341 None:intergenic 40.0%
AGCGGAATAGGATATGGAAG+TGG - chr4.4:51351293-51351312 MS.gene044732:CDS 45.0%
GACTCCATTCAGTTCCTCAA+TGG + chr4.4:51350829-51350848 None:intergenic 45.0%
GCATTTACAACGAGAAAGGC+TGG - chr4.4:51350282-51350301 MS.gene044732:CDS 45.0%
GGTGTAAGCGGAATAGGATA+TGG - chr4.4:51351287-51351306 MS.gene044732:CDS 45.0%
! AATGTGTGAGTGTTGCCGCT+AGG + chr4.4:51350963-51350982 None:intergenic 50.0%
GCGCCGAAGAGGTCATCAAA+AGG - chr4.4:51350207-51350226 MS.gene044732:CDS 55.0%
GGAGGAGAGATTGACCCTCA+TGG - chr4.4:51350675-51350694 MS.gene044732:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 51350204 51351343 51350204 ID=MS.gene044732
chr4.4 mRNA 51350204 51351343 51350204 ID=MS.gene044732.t1;Parent=MS.gene044732
chr4.4 exon 51350851 51351343 51350851 ID=MS.gene044732.t1.exon1;Parent=MS.gene044732.t1
chr4.4 CDS 51350851 51351343 51350851 ID=cds.MS.gene044732.t1;Parent=MS.gene044732.t1
chr4.4 exon 51350641 51350730 51350641 ID=MS.gene044732.t1.exon2;Parent=MS.gene044732.t1
chr4.4 CDS 51350641 51350730 51350641 ID=cds.MS.gene044732.t1;Parent=MS.gene044732.t1
chr4.4 exon 51350204 51350328 51350204 ID=MS.gene044732.t1.exon3;Parent=MS.gene044732.t1
chr4.4 CDS 51350204 51350328 51350204 ID=cds.MS.gene044732.t1;Parent=MS.gene044732.t1
Gene Sequence

>MS.gene044732

ATGGCGCCGAAGAGGTCATCAAAAGGAAAACAAAAAATTGAGTTGAAAAAAATTGAGAAAAAGGATGATCTCTTTGTTGCATTTACAACGAGAAAGGCTGGAATTTACAACAAGGCCTCCGAGTTAACAACACTTTCAGGGGCCTCAGTTGATATTCTCATGCTTTCTCCAACGGGAAAACCATTTTGTTATGGAAACCCATCTTCTGAATCTATAACAAAAACTAGTTTGAAGGATGAAAATCCTTCAATGGAAGTGGAAATGATGAAACCGGAGATTATAGAGCAAAATGAAAAAAATGATGATCTTTTGGATAAAAAGCATGTTGCGGAAGCACAACGTAAAAATTTGAAGAAGTTGGAAAGTTCTGGATATTGGTCGATAAAAGAGGACCAATATGATCAAGTGAAAGAAATTGACAAATCCTTAGTTGCTGTCGTAAATAAATTGATAACCAATGTCTTGAAAACTGGAGGAGAGATTGACCCTCATGGTATGTGTACATCTTGTAACTATCTATGTGTTATATTTGATCTATTAATGACGTCAAATGATTTCATGGCATCCTTTTTACCTAATTATTTTAAGTGAATCTGGTTATAATTTTTCGTAGCAATTTCTACCATTGAGGAACTGAATGGAGTCAAAACAAATCCTTCTTTTGTCTTTGAGGACAACACCAAAGATCATGAAGTTGGTGCAAGTAAGAATTTTCATTCATGTTTTTAATTTTTTTATTTTTTTTTCATGAAATATCCTAGCGGCAACACTCACACATTAAACTTTTAGACAAGATCAGATGTTGTTGGATAATCATGTCCCAACACATCAAATATCTCTAGATAAAACTTTTACCATTTAAGTTGCACTTATGGGAAAAATATCAATTCTTTTTCTTCTTAATTTCCATTACTAGGCATGCAAACATTTACTTTTAATATTTCATGCGACAAACCTAACATATATATTATGTATTGTCTTTCCTTTCATTGATTCAATTTTTTTTATCCAAAAGGTAAAGGTATGGTTGCTAAAGCCTCTCAAGATATAGCTACAAAAAAAACACAAGGAGTCAACCTTATTGGTGTAAGCGGAATAGGATATGGAAGTGGTGTTGATCATGCTTCTCCAGATATATGA

Protein sequence

>MS.gene044732.t1

MAPKRSSKGKQKIELKKIEKKDDLFVAFTTRKAGIYNKASELTTLSGASVDILMLSPTGKPFCYGNPSSESITKTSLKDENPSMEVEMMKPEIIEQNEKNDDLLDKKHVAEAQRKNLKKLESSGYWSIKEDQYDQVKEIDKSLVAVVNKLITNVLKTGGEIDPHAISTIEELNGVKTNPSFVFEDNTKDHEVGASKGMVAKASQDIATKKTQGVNLIGVSGIGYGSGVDHASPDI