Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044765.t1 | XP_003606099.1 | 94.9 | 176 | 9 | 0 | 1 | 176 | 1 | 176 | 5.20E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044765.t1 | Q3EAL1 | 44.6 | 101 | 53 | 2 | 77 | 176 | 43 | 141 | 5.0e-16 | 85.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044765.t1 | G7JHA0 | 94.9 | 176 | 9 | 0 | 1 | 176 | 1 | 176 | 3.7e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044765.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044765.t1 | MTR_4g052040 | 94.886 | 176 | 9 | 0 | 1 | 176 | 1 | 176 | 1.09e-114 | 323 |
| MS.gene044765.t1 | MTR_4g052060 | 69.613 | 181 | 43 | 5 | 1 | 176 | 1 | 174 | 4.21e-79 | 233 |
| MS.gene044765.t1 | MTR_5g079710 | 46.988 | 166 | 76 | 5 | 4 | 168 | 6 | 160 | 1.79e-41 | 140 |
| MS.gene044765.t1 | MTR_7g105790 | 35.294 | 187 | 103 | 4 | 1 | 176 | 1 | 180 | 2.51e-26 | 99.8 |
| MS.gene044765.t1 | MTR_0907s0020 | 62.319 | 69 | 26 | 0 | 101 | 169 | 104 | 172 | 3.76e-25 | 96.3 |
| MS.gene044765.t1 | MTR_5g075210 | 53.030 | 66 | 31 | 0 | 97 | 162 | 278 | 343 | 4.68e-15 | 72.0 |
| MS.gene044765.t1 | MTR_8g103520 | 44.156 | 77 | 43 | 0 | 92 | 168 | 217 | 293 | 5.64e-15 | 71.2 |
| MS.gene044765.t1 | MTR_7g072875 | 45.070 | 71 | 39 | 0 | 90 | 160 | 210 | 280 | 5.49e-14 | 68.6 |
| MS.gene044765.t1 | MTR_4g028930 | 49.180 | 61 | 31 | 0 | 100 | 160 | 315 | 375 | 6.92e-14 | 68.9 |
| MS.gene044765.t1 | MTR_7g072630 | 45.070 | 71 | 39 | 0 | 90 | 160 | 272 | 342 | 7.60e-14 | 68.6 |
| MS.gene044765.t1 | MTR_2g018030 | 40.000 | 65 | 39 | 0 | 96 | 160 | 274 | 338 | 1.52e-11 | 62.0 |
| MS.gene044765.t1 | MTR_3g111780 | 46.835 | 79 | 42 | 0 | 86 | 164 | 185 | 263 | 2.79e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044765.t1 | AT3G52525 | 63.333 | 60 | 22 | 0 | 100 | 159 | 67 | 126 | 4.69e-22 | 87.4 |
| MS.gene044765.t1 | AT2G36026 | 57.377 | 61 | 26 | 0 | 104 | 164 | 91 | 151 | 1.16e-18 | 79.3 |
| MS.gene044765.t1 | AT5G19650 | 50.000 | 66 | 33 | 0 | 99 | 164 | 154 | 219 | 1.97e-15 | 71.6 |
| MS.gene044765.t1 | AT5G22240 | 44.595 | 74 | 39 | 1 | 100 | 171 | 97 | 170 | 1.39e-14 | 68.9 |
| MS.gene044765.t1 | AT1G06920 | 39.759 | 83 | 50 | 0 | 82 | 164 | 226 | 308 | 4.55e-14 | 68.9 |
| MS.gene044765.t1 | AT2G18500 | 46.875 | 64 | 34 | 0 | 97 | 160 | 224 | 287 | 9.38e-14 | 68.2 |
| MS.gene044765.t1 | AT5G58360 | 44.118 | 68 | 38 | 0 | 93 | 160 | 218 | 285 | 1.02e-13 | 67.8 |
| MS.gene044765.t1 | AT2G30400 | 46.875 | 64 | 34 | 0 | 97 | 160 | 250 | 313 | 4.23e-13 | 66.2 |
| MS.gene044765.t1 | AT5G01840 | 51.724 | 58 | 28 | 0 | 103 | 160 | 202 | 259 | 9.07e-13 | 65.1 |
| MS.gene044765.t1 | AT4G18830 | 46.552 | 58 | 31 | 0 | 102 | 159 | 285 | 342 | 3.13e-11 | 61.2 |
Find 33 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCATTAGTGGGCTTTGTT+TGG | 0.342399 | 4.1:+45881226 | None:intergenic |
| CTCAATTCTAGTTGTTATCA+TGG | 0.388006 | 4.1:-45881074 | MS.gene044765:CDS |
| GCAGTGCTACCAACCTTGTC+CGG | 0.389497 | 4.1:+45881008 | None:intergenic |
| CATATGTCATGAAGCTTTCC+CGG | 0.390173 | 4.1:-45881027 | MS.gene044765:CDS |
| TTTCTCCACCACGACGTTCT+CGG | 0.395337 | 4.1:-45881279 | MS.gene044765:CDS |
| TTCATTAGTGGGCTTTGTTT+GGG | 0.402789 | 4.1:+45881227 | None:intergenic |
| GATCACAAACCCAAGTAGTA+AGG | 0.415466 | 4.1:-45881366 | MS.gene044765:CDS |
| GGTTCTTCAACTACAACTTC+AGG | 0.425708 | 4.1:-45881344 | MS.gene044765:CDS |
| ACAACTTCAGGTGAAGGATA+TGG | 0.425803 | 4.1:-45881332 | MS.gene044765:CDS |
| GTTGAAGAACCCTTACTACT+TGG | 0.435315 | 4.1:+45881356 | None:intergenic |
| ACCGCCACCGTGTTCATTAG+TGG | 0.446501 | 4.1:+45881215 | None:intergenic |
| CTATCGTCGATGCTACAAAC+GGG | 0.480286 | 4.1:+45881308 | None:intergenic |
| CGAGCCAAAGCCAACTCAAA+AGG | 0.488426 | 4.1:-45881411 | MS.gene044765:CDS |
| TTCACATCAAATGGAAGTTG+TGG | 0.499142 | 4.1:-45881464 | MS.gene044765:CDS |
| ATGATCTTCAAGATCTTCTC+CGG | 0.511298 | 4.1:-45881109 | MS.gene044765:CDS |
| ACTATCGTCGATGCTACAAA+CGG | 0.524855 | 4.1:+45881307 | None:intergenic |
| CCGCCACCGTGTTCATTAGT+GGG | 0.525048 | 4.1:+45881216 | None:intergenic |
| TTGAAGAACCCTTACTACTT+GGG | 0.531511 | 4.1:+45881357 | None:intergenic |
| TCATGAAGCTTTCCCGGACA+AGG | 0.542749 | 4.1:-45881021 | MS.gene044765:CDS |
| CAGTGCTACCAACCTTGTCC+GGG | 0.561453 | 4.1:+45881009 | None:intergenic |
| GAACACGGTGGCGGTAGAGA+AGG | 0.563314 | 4.1:-45881207 | MS.gene044765:CDS |
| GAATGTTTGAAATCATGATA+CGG | 0.577874 | 4.1:+45881173 | None:intergenic |
| TCAACTACAACTTCAGGTGA+AGG | 0.579124 | 4.1:-45881338 | MS.gene044765:CDS |
| GAAGCTTTCCCGGACAAGGT+TGG | 0.580453 | 4.1:-45881017 | MS.gene044765:CDS |
| ATCACAAACCCAAGTAGTAA+GGG | 0.581401 | 4.1:-45881365 | MS.gene044765:CDS |
| AATTGAGATGAAGAAAACAC+CGG | 0.628303 | 4.1:+45881090 | None:intergenic |
| CCCACTAATGAACACGGTGG+CGG | 0.643057 | 4.1:-45881216 | MS.gene044765:CDS |
| GACATCCGAGAACGTCGTGG+TGG | 0.662804 | 4.1:+45881274 | None:intergenic |
| GGCGGTAGAGAAGGACTCTG+AGG | 0.686815 | 4.1:-45881198 | MS.gene044765:CDS |
| ACAAAGCCCACTAATGAACA+CGG | 0.697529 | 4.1:-45881222 | MS.gene044765:CDS |
| TATCGTCGATGCTACAAACG+GGG | 0.740115 | 4.1:+45881309 | None:intergenic |
| GCTGACATCCGAGAACGTCG+TGG | 0.753322 | 4.1:+45881271 | None:intergenic |
| AAGCCCACTAATGAACACGG+TGG | 0.804493 | 4.1:-45881219 | MS.gene044765:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTGATTAATAGAAAATT+TGG | + | chr4.1:45881104-45881123 | None:intergenic | 15.0% |
| !! | ATGTGAAAAATGTTCTTATA+AGG | + | chr4.1:45881012-45881031 | None:intergenic | 20.0% |
| !! | AGAACATTTTTCACATCAAA+TGG | - | chr4.1:45881016-45881035 | MS.gene044765:CDS | 25.0% |
| !! | GAATGTTTGAAATCATGATA+CGG | + | chr4.1:45881319-45881338 | None:intergenic | 25.0% |
| !! | TCTTATAAGGTTTTTCTTGT+TGG | + | chr4.1:45880999-45881018 | None:intergenic | 25.0% |
| AATTGAGATGAAGAAAACAC+CGG | + | chr4.1:45881402-45881421 | None:intergenic | 30.0% | |
| ! | CTCAATTCTAGTTGTTATCA+TGG | - | chr4.1:45881415-45881434 | MS.gene044765:CDS | 30.0% |
| ATCACAAACCCAAGTAGTAA+GGG | - | chr4.1:45881124-45881143 | MS.gene044765:CDS | 35.0% | |
| ATGATCTTCAAGATCTTCTC+CGG | - | chr4.1:45881380-45881399 | MS.gene044765:CDS | 35.0% | |
| TTCACATCAAATGGAAGTTG+TGG | - | chr4.1:45881025-45881044 | MS.gene044765:CDS | 35.0% | |
| TTGAAGAACCCTTACTACTT+GGG | + | chr4.1:45881135-45881154 | None:intergenic | 35.0% | |
| !! | GAAAATTTGGCCTTTTGAGT+TGG | + | chr4.1:45881091-45881110 | None:intergenic | 35.0% |
| !! | TTCATTAGTGGGCTTTGTTT+GGG | + | chr4.1:45881265-45881284 | None:intergenic | 35.0% |
| ACAAAGCCCACTAATGAACA+CGG | - | chr4.1:45881267-45881286 | MS.gene044765:CDS | 40.0% | |
| ACAACTTCAGGTGAAGGATA+TGG | - | chr4.1:45881157-45881176 | MS.gene044765:CDS | 40.0% | |
| ACTATCGTCGATGCTACAAA+CGG | + | chr4.1:45881185-45881204 | None:intergenic | 40.0% | |
| CATATGTCATGAAGCTTTCC+CGG | - | chr4.1:45881462-45881481 | MS.gene044765:CDS | 40.0% | |
| GATCACAAACCCAAGTAGTA+AGG | - | chr4.1:45881123-45881142 | MS.gene044765:CDS | 40.0% | |
| GGTTCTTCAACTACAACTTC+AGG | - | chr4.1:45881145-45881164 | MS.gene044765:CDS | 40.0% | |
| GTTGAAGAACCCTTACTACT+TGG | + | chr4.1:45881136-45881155 | None:intergenic | 40.0% | |
| TCAACTACAACTTCAGGTGA+AGG | - | chr4.1:45881151-45881170 | MS.gene044765:CDS | 40.0% | |
| !! | GTTCATTAGTGGGCTTTGTT+TGG | + | chr4.1:45881266-45881285 | None:intergenic | 40.0% |
| CTATCGTCGATGCTACAAAC+GGG | + | chr4.1:45881184-45881203 | None:intergenic | 45.0% | |
| TATCGTCGATGCTACAAACG+GGG | + | chr4.1:45881183-45881202 | None:intergenic | 45.0% | |
| !!! | TTGGCCTTTTGAGTTGGCTT+TGG | + | chr4.1:45881085-45881104 | None:intergenic | 45.0% |
| AAGCCCACTAATGAACACGG+TGG | - | chr4.1:45881270-45881289 | MS.gene044765:CDS | 50.0% | |
| CGAGCCAAAGCCAACTCAAA+AGG | - | chr4.1:45881078-45881097 | MS.gene044765:CDS | 50.0% | |
| TCATGAAGCTTTCCCGGACA+AGG | - | chr4.1:45881468-45881487 | MS.gene044765:CDS | 50.0% | |
| TTTCTCCACCACGACGTTCT+CGG | - | chr4.1:45881210-45881229 | MS.gene044765:CDS | 50.0% | |
| ACCGCCACCGTGTTCATTAG+TGG | + | chr4.1:45881277-45881296 | None:intergenic | 55.0% | |
| CAGTGCTACCAACCTTGTCC+GGG | + | chr4.1:45881483-45881502 | None:intergenic | 55.0% | |
| CCCACTAATGAACACGGTGG+CGG | - | chr4.1:45881273-45881292 | MS.gene044765:CDS | 55.0% | |
| CCGCCACCGTGTTCATTAGT+GGG | + | chr4.1:45881276-45881295 | None:intergenic | 55.0% | |
| GAAGCTTTCCCGGACAAGGT+TGG | - | chr4.1:45881472-45881491 | MS.gene044765:CDS | 55.0% | |
| GCAGTGCTACCAACCTTGTC+CGG | + | chr4.1:45881484-45881503 | None:intergenic | 55.0% | |
| GACATCCGAGAACGTCGTGG+TGG | + | chr4.1:45881218-45881237 | None:intergenic | 60.0% | |
| GCTGACATCCGAGAACGTCG+TGG | + | chr4.1:45881221-45881240 | None:intergenic | 60.0% | |
| GGCGGTAGAGAAGGACTCTG+AGG | - | chr4.1:45881291-45881310 | MS.gene044765:CDS | 60.0% | |
| ! | GAACACGGTGGCGGTAGAGA+AGG | - | chr4.1:45881282-45881301 | MS.gene044765:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 45880989 | 45881522 | 45880989 | ID=MS.gene044765 |
| chr4.1 | mRNA | 45880989 | 45881522 | 45880989 | ID=MS.gene044765.t1;Parent=MS.gene044765 |
| chr4.1 | exon | 45880989 | 45881522 | 45880989 | ID=MS.gene044765.t1.exon1;Parent=MS.gene044765.t1 |
| chr4.1 | CDS | 45880989 | 45881522 | 45880989 | ID=cds.MS.gene044765.t1;Parent=MS.gene044765.t1 |
| Gene Sequence |
| Protein sequence |
