Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044945.t1 | XP_003621899.2 | 94.3 | 176 | 10 | 0 | 1 | 176 | 1 | 176 | 2.90E-84 | 321.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044945.t1 | P05332 | 28.5 | 172 | 115 | 4 | 5 | 169 | 5 | 175 | 6.3e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044945.t1 | G7KXV2 | 94.3 | 176 | 10 | 0 | 1 | 176 | 1 | 176 | 2.1e-84 | 321.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044945.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044945.t1 | MTR_7g024760 | 95.455 | 176 | 8 | 0 | 1 | 176 | 1 | 176 | 7.91e-127 | 353 |
| MS.gene044945.t1 | MTR_7g024765 | 84.393 | 173 | 27 | 0 | 1 | 173 | 1 | 173 | 1.19e-106 | 303 |
| MS.gene044945.t1 | MTR_7g024780 | 68.208 | 173 | 54 | 1 | 1 | 172 | 1 | 173 | 9.73e-84 | 244 |
| MS.gene044945.t1 | MTR_7g114410 | 59.195 | 174 | 69 | 2 | 1 | 172 | 1 | 174 | 8.60e-69 | 207 |
| MS.gene044945.t1 | MTR_7g114410 | 58.553 | 152 | 61 | 2 | 7 | 156 | 7 | 158 | 4.51e-59 | 181 |
| MS.gene044945.t1 | MTR_3g463610 | 50.595 | 168 | 78 | 2 | 7 | 169 | 6 | 173 | 9.84e-54 | 170 |
| MS.gene044945.t1 | MTR_1g103110 | 44.512 | 164 | 88 | 3 | 7 | 167 | 22 | 185 | 3.43e-41 | 137 |
| MS.gene044945.t1 | MTR_5g006470 | 42.771 | 166 | 92 | 2 | 7 | 169 | 20 | 185 | 5.97e-39 | 131 |
| MS.gene044945.t1 | MTR_5g006460 | 42.105 | 171 | 90 | 6 | 7 | 169 | 21 | 190 | 3.65e-38 | 129 |
| MS.gene044945.t1 | MTR_5g006510 | 42.857 | 168 | 90 | 4 | 7 | 169 | 22 | 188 | 4.28e-38 | 129 |
| MS.gene044945.t1 | MTR_1g103150 | 41.566 | 166 | 94 | 3 | 7 | 169 | 22 | 187 | 2.89e-37 | 127 |
| MS.gene044945.t1 | MTR_5g006450 | 41.071 | 168 | 94 | 3 | 7 | 169 | 20 | 187 | 8.64e-37 | 126 |
| MS.gene044945.t1 | MTR_5g006520 | 39.881 | 168 | 96 | 3 | 7 | 169 | 1738 | 1905 | 2.29e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044945.t1 | AT2G32020 | 39.884 | 173 | 100 | 3 | 1 | 171 | 12 | 182 | 7.34e-45 | 146 |
| MS.gene044945.t1 | AT3G22560 | 44.578 | 166 | 88 | 3 | 8 | 169 | 7 | 172 | 4.95e-43 | 141 |
| MS.gene044945.t1 | AT2G32030 | 40.361 | 166 | 95 | 3 | 8 | 171 | 24 | 187 | 1.07e-42 | 141 |
Find 43 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTATTGGGTTCAACTATT+GGG | 0.213071 | 7.1:+73043738 | MS.gene044945:CDS |
| GCAAAGTTTAAAGCAGATTT+AGG | 0.222691 | 7.1:+73043712 | MS.gene044945:CDS |
| ATGCTATTGGGTTCAACTAT+TGG | 0.239859 | 7.1:+73043737 | MS.gene044945:CDS |
| TCCTGATTTGGTAAGGTTGC+AGG | 0.261225 | 7.1:+73043819 | MS.gene044945:CDS |
| GCAGATTTAGGCTATGCTAT+TGG | 0.284212 | 7.1:+73043724 | MS.gene044945:CDS |
| ATTCCAAGACTTTCCTGATT+TGG | 0.295374 | 7.1:+73043807 | MS.gene044945:CDS |
| TGTCTTAATGATCACTCTAT+TGG | 0.321892 | 7.1:+73043661 | MS.gene044945:CDS |
| CAGATTTAGGCTATGCTATT+GGG | 0.336174 | 7.1:+73043725 | MS.gene044945:CDS |
| TTCTGAACAATCCCTCCCTT+TGG | 0.370350 | 7.1:-73043897 | None:intergenic |
| TTAAACTTTGCATCAGGATA+AGG | 0.382959 | 7.1:-73043700 | None:intergenic |
| CAAGAGAATCTTCACTGCTT+TGG | 0.386713 | 7.1:-73043776 | None:intergenic |
| CATTAAGACATATTGATTGT+TGG | 0.387585 | 7.1:-73043648 | None:intergenic |
| GCCTGCAACCTTACCAAATC+AGG | 0.389988 | 7.1:-73043820 | None:intergenic |
| TCAGCATCACTTTCTTTGAA+TGG | 0.407648 | 7.1:-73043511 | None:intergenic |
| AGACATATTGATTGTTGGAA+TGG | 0.412290 | 7.1:-73043643 | None:intergenic |
| GAAAGGGAATTTATAACAAA+GGG | 0.412673 | 7.1:+73043917 | MS.gene044945:CDS |
| TCTGCTTTAAACTTTGCATC+AGG | 0.422143 | 7.1:-73043706 | None:intergenic |
| CTGCTTTGGTGGCTACACCT+TGG | 0.450078 | 7.1:-73043762 | None:intergenic |
| CATCACTTTCTTTGAATGGC+CGG | 0.455673 | 7.1:-73043507 | None:intergenic |
| AAGACTTTCCTGATTTGGTA+AGG | 0.487902 | 7.1:+73043812 | MS.gene044945:CDS |
| TTATAACAAAGGGAATATAG+AGG | 0.497416 | 7.1:+73043927 | MS.gene044945:CDS |
| TTACCAAATCAGGAAAGTCT+TGG | 0.502664 | 7.1:-73043810 | None:intergenic |
| CGATGAAATTCCTCATGCTA+AGG | 0.513932 | 7.1:+73043978 | MS.gene044945:CDS |
| GGGTTCAACTATTGGGGCCA+AGG | 0.514130 | 7.1:+73043745 | MS.gene044945:CDS |
| GATTGGAGTTATGTAAATCA+AGG | 0.516459 | 7.1:+73043581 | MS.gene044945:CDS |
| GAGAATCTTCACTGCTTTGG+TGG | 0.530304 | 7.1:-73043773 | None:intergenic |
| AGAAAGGGAATTTATAACAA+AGG | 0.541022 | 7.1:+73043916 | MS.gene044945:CDS |
| GATGAAATTCCTCATGCTAA+GGG | 0.544681 | 7.1:+73043979 | MS.gene044945:CDS |
| TGGAAAAGGTTGGTTTCCAA+AGG | 0.552140 | 7.1:+73043881 | MS.gene044945:CDS |
| AGGGAGGGATTGTTCAGAAA+GGG | 0.555894 | 7.1:+73043901 | MS.gene044945:CDS |
| AGTCATGCAATATATGCGAT+TGG | 0.560410 | 7.1:+73043564 | MS.gene044945:CDS |
| GGAAAAGGTTGGTTTCCAAA+GGG | 0.561564 | 7.1:+73043882 | MS.gene044945:CDS |
| GCTATTGGGTTCAACTATTG+GGG | 0.562007 | 7.1:+73043739 | MS.gene044945:CDS |
| AAAGGTTGGTTTCCAAAGGG+AGG | 0.574627 | 7.1:+73043885 | MS.gene044945:CDS |
| TAATACTCGTCCAGAGAACA+TGG | 0.576553 | 7.1:+73043843 | MS.gene044945:CDS |
| ATGAGTCAAGGATCACTCTC+CGG | 0.583353 | 7.1:+73043488 | MS.gene044945:CDS |
| AAGGGAGGGATTGTTCAGAA+AGG | 0.588499 | 7.1:+73043900 | MS.gene044945:CDS |
| ATTGGAGTTATGTAAATCAA+GGG | 0.589655 | 7.1:+73043582 | MS.gene044945:CDS |
| AAGGTTGGTTTCCAAAGGGA+GGG | 0.591625 | 7.1:+73043886 | MS.gene044945:CDS |
| ACTAATTAACCCTTAGCATG+AGG | 0.612515 | 7.1:-73043988 | None:intergenic |
| CTTAGCATGAGGAATTTCAT+CGG | 0.640114 | 7.1:-73043977 | None:intergenic |
| TGGCAATAGATCATGAGTCA+AGG | 0.642231 | 7.1:+73043476 | MS.gene044945:CDS |
| ACTTTGCATCAGGATAAGGA+AGG | 0.674910 | 7.1:-73043696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAAAGGGAATTTATAACAA+AGG | + | chr7.1:73043916-73043935 | MS.gene044945:CDS | 25.0% |
| ! | ATTGGAGTTATGTAAATCAA+GGG | + | chr7.1:73043582-73043601 | MS.gene044945:CDS | 25.0% |
| ! | GAAAGGGAATTTATAACAAA+GGG | + | chr7.1:73043917-73043936 | MS.gene044945:CDS | 25.0% |
| ! | TTATAACAAAGGGAATATAG+AGG | + | chr7.1:73043927-73043946 | MS.gene044945:CDS | 25.0% |
| !! | CATTAAGACATATTGATTGT+TGG | - | chr7.1:73043651-73043670 | None:intergenic | 25.0% |
| GATTGGAGTTATGTAAATCA+AGG | + | chr7.1:73043581-73043600 | MS.gene044945:CDS | 30.0% | |
| TGTCTTAATGATCACTCTAT+TGG | + | chr7.1:73043661-73043680 | MS.gene044945:CDS | 30.0% | |
| TTAAACTTTGCATCAGGATA+AGG | - | chr7.1:73043703-73043722 | None:intergenic | 30.0% | |
| TTCTCAAAAACTGTTGGAAA+AGG | + | chr7.1:73043867-73043886 | MS.gene044945:CDS | 30.0% | |
| ! | AGACATATTGATTGTTGGAA+TGG | - | chr7.1:73043646-73043665 | None:intergenic | 30.0% |
| ! | GCAAAGTTTAAAGCAGATTT+AGG | + | chr7.1:73043712-73043731 | MS.gene044945:CDS | 30.0% |
| AGTCATGCAATATATGCGAT+TGG | + | chr7.1:73043564-73043583 | MS.gene044945:CDS | 35.0% | |
| ATGCTATTGGGTTCAACTAT+TGG | + | chr7.1:73043737-73043756 | MS.gene044945:CDS | 35.0% | |
| ATTCCAAGACTTTCCTGATT+TGG | + | chr7.1:73043807-73043826 | MS.gene044945:CDS | 35.0% | |
| CAAAAACTGTTGGAAAAGGT+TGG | + | chr7.1:73043871-73043890 | MS.gene044945:CDS | 35.0% | |
| CAGATTTAGGCTATGCTATT+GGG | + | chr7.1:73043725-73043744 | MS.gene044945:CDS | 35.0% | |
| CTTAGCATGAGGAATTTCAT+CGG | - | chr7.1:73043980-73043999 | None:intergenic | 35.0% | |
| GATGAAATTCCTCATGCTAA+GGG | + | chr7.1:73043979-73043998 | MS.gene044945:CDS | 35.0% | |
| TCAGCATCACTTTCTTTGAA+TGG | - | chr7.1:73043514-73043533 | None:intergenic | 35.0% | |
| TGCTATTGGGTTCAACTATT+GGG | + | chr7.1:73043738-73043757 | MS.gene044945:CDS | 35.0% | |
| TTACCAAATCAGGAAAGTCT+TGG | - | chr7.1:73043813-73043832 | None:intergenic | 35.0% | |
| ! | TCTGCTTTAAACTTTGCATC+AGG | - | chr7.1:73043709-73043728 | None:intergenic | 35.0% |
| !! | AAGACTTTCCTGATTTGGTA+AGG | + | chr7.1:73043812-73043831 | MS.gene044945:CDS | 35.0% |
| !! | ATGCTGATGATGTTTTGTTG+TGG | + | chr7.1:73043527-73043546 | MS.gene044945:CDS | 35.0% |
| !! | TGCTGATGATGTTTTGTTGT+GGG | + | chr7.1:73043528-73043547 | MS.gene044945:CDS | 35.0% |
| ACTTTGCATCAGGATAAGGA+AGG | - | chr7.1:73043699-73043718 | None:intergenic | 40.0% | |
| CAAGAGAATCTTCACTGCTT+TGG | - | chr7.1:73043779-73043798 | None:intergenic | 40.0% | |
| CATCACTTTCTTTGAATGGC+CGG | - | chr7.1:73043510-73043529 | None:intergenic | 40.0% | |
| CATGGCTTCTCAAAAACTGT+TGG | + | chr7.1:73043861-73043880 | MS.gene044945:CDS | 40.0% | |
| CGATGAAATTCCTCATGCTA+AGG | + | chr7.1:73043978-73043997 | MS.gene044945:CDS | 40.0% | |
| GCAGATTTAGGCTATGCTAT+TGG | + | chr7.1:73043724-73043743 | MS.gene044945:CDS | 40.0% | |
| GCTATTGGGTTCAACTATTG+GGG | + | chr7.1:73043739-73043758 | MS.gene044945:CDS | 40.0% | |
| TAATACTCGTCCAGAGAACA+TGG | + | chr7.1:73043843-73043862 | MS.gene044945:CDS | 40.0% | |
| TGGCAATAGATCATGAGTCA+AGG | + | chr7.1:73043476-73043495 | MS.gene044945:CDS | 40.0% | |
| !! | GGAAAAGGTTGGTTTCCAAA+GGG | + | chr7.1:73043882-73043901 | MS.gene044945:CDS | 40.0% |
| !! | TGGAAAAGGTTGGTTTCCAA+AGG | + | chr7.1:73043881-73043900 | MS.gene044945:CDS | 40.0% |
| !! | TTTTGAGAAGCCATGTTCTC+TGG | - | chr7.1:73043856-73043875 | None:intergenic | 40.0% |
| AAGGGAGGGATTGTTCAGAA+AGG | + | chr7.1:73043900-73043919 | MS.gene044945:CDS | 45.0% | |
| AGGGAGGGATTGTTCAGAAA+GGG | + | chr7.1:73043901-73043920 | MS.gene044945:CDS | 45.0% | |
| ATGAGTCAAGGATCACTCTC+CGG | + | chr7.1:73043488-73043507 | MS.gene044945:CDS | 45.0% | |
| GAGAATCTTCACTGCTTTGG+TGG | - | chr7.1:73043776-73043795 | None:intergenic | 45.0% | |
| TTCTGAACAATCCCTCCCTT+TGG | - | chr7.1:73043900-73043919 | None:intergenic | 45.0% | |
| !! | AAAGGTTGGTTTCCAAAGGG+AGG | + | chr7.1:73043885-73043904 | MS.gene044945:CDS | 45.0% |
| !! | AAGGTTGGTTTCCAAAGGGA+GGG | + | chr7.1:73043886-73043905 | MS.gene044945:CDS | 45.0% |
| !! | TCCTGATTTGGTAAGGTTGC+AGG | + | chr7.1:73043819-73043838 | MS.gene044945:CDS | 45.0% |
| GCCTGCAACCTTACCAAATC+AGG | - | chr7.1:73043823-73043842 | None:intergenic | 50.0% | |
| GGGTTCAACTATTGGGGCCA+AGG | + | chr7.1:73043745-73043764 | MS.gene044945:CDS | 55.0% | |
| ! | CTGCTTTGGTGGCTACACCT+TGG | - | chr7.1:73043765-73043784 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 73043475 | 73044005 | 73043475 | ID=MS.gene044945 |
| chr7.1 | mRNA | 73043475 | 73044005 | 73043475 | ID=MS.gene044945.t1;Parent=MS.gene044945 |
| chr7.1 | exon | 73043475 | 73044005 | 73043475 | ID=MS.gene044945.t1.exon1;Parent=MS.gene044945.t1 |
| chr7.1 | CDS | 73043475 | 73044005 | 73043475 | ID=cds.MS.gene044945.t1;Parent=MS.gene044945.t1 |
| Gene Sequence |
| Protein sequence |
