AlfalfaGEDB | MS.gene044961

M. sativa cultivar XinJiangDaYe / MS.gene044961

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044961.t1	KEH26852.1	60.3	63	25	0	1	63	82	144	8.10E-14	85.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044961.t1	A0A072UB72	60.3	63	25	0	1	63	82	144	5.8e-14	85.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene044961.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene044961.t1	MTR_6g477950	60.317	63	25	0	1	63	82	144	1.72e-21	85.1
MS.gene044961.t1	MTR_7g068970	64.615	65	14	2	1	63	27	84	1.04e-19	77.4
MS.gene044961.t1	MTR_6g034285	88.889	36	4	0	11	46	365	400	9.26e-17	72.4
MS.gene044961.t1	MTR_8g069780	49.206	63	32	0	1	63	93	155	8.57e-14	62.0
MS.gene044961.t1	MTR_5g071410	48.438	64	33	0	1	64	206	269	5.74e-13	61.6
MS.gene044961.t1	MTR_7g117585	45.312	64	35	0	1	64	83	146	6.37e-13	59.7
MS.gene044961.t1	MTR_3g117130	52.941	51	24	0	1	51	216	266	8.71e-12	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTTGGTTCCAGTTTGTT+AGG	0.284226	7.1:-73263047	MS.gene044961:CDS
CGACAAGCTGCTATTCGATT+TGG	0.289962	7.1:-73263004	MS.gene044961:CDS
GACAAGCTGCTATTCGATTT+GGG	0.344086	7.1:-73263003	MS.gene044961:CDS
GCAGCTTGTCGCCCTCTTGT+AGG	0.397478	7.1:+73263016	None:intergenic
TCCTACAATGTTATTTGTTG+AGG	0.406389	7.1:-73262974	MS.gene044961:CDS
GATTGATGTGCTTACCGGTC+AGG	0.408212	7.1:-73263127	MS.gene044961:CDS
TACAATGTTATTTGTTGAGG+TGG	0.413661	7.1:-73262971	MS.gene044961:CDS
CAGTTGATTGATGTGCTTAC+CGG	0.428124	7.1:-73263132	MS.gene044961:CDS
GGTGATTGTCCCTAACAAAC+TGG	0.445103	7.1:+73263037	None:intergenic
AGGTTGGTTCCAGTTTGTTA+GGG	0.493750	7.1:-73263046	MS.gene044961:CDS
TTCAGCCTTGTTACGCTCAA+CGG	0.503360	7.1:+73263083	None:intergenic
ACCTCAACAAATAACATTGT+AGG	0.513439	7.1:+73262973	None:intergenic
TGTTACGCTCAACGGTAATG+AGG	0.584146	7.1:+73263091	None:intergenic
CATTACCGTTGAGCGTAACA+AGG	0.590910	7.1:-73263088	MS.gene044961:CDS
TCAACAAATAACATTGTAGG+AGG	0.591551	7.1:+73262976	None:intergenic
CTGGAACCAACCTTCTCCCA+AGG	0.599362	7.1:+73263056	None:intergenic
GCGGCAATCGTATTCCTGAC+CGG	0.622702	7.1:+73263113	None:intergenic
TAGGGACAATCACCTACAAG+AGG	0.660602	7.1:-73263028	MS.gene044961:CDS
TACGCTCAACGGTAATGAGG+CGG	0.676660	7.1:+73263094	None:intergenic
AGGGACAATCACCTACAAGA+GGG	0.691250	7.1:-73263027	MS.gene044961:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACCTCAACAAATAACATTGT+AGG	+	chr7.1:73263113-73263132	None:intergenic	30.0%
	TCAACAAATAACATTGTAGG+AGG	+	chr7.1:73263110-73263129	None:intergenic	30.0%
!	TACAATGTTATTTGTTGAGG+TGG	-	chr7.1:73263112-73263131	MS.gene044961:CDS	30.0%
!	TCCTACAATGTTATTTGTTG+AGG	-	chr7.1:73263109-73263128	MS.gene044961:CDS	30.0%
	AACAAGGCTGAAAAATCCTT+GGG	-	chr7.1:73263011-73263030	MS.gene044961:CDS	35.0%
	TAACAAGGCTGAAAAATCCT+TGG	-	chr7.1:73263010-73263029	MS.gene044961:CDS	35.0%
	AAAAATCCTTGGGAGAAGGT+TGG	-	chr7.1:73263021-73263040	MS.gene044961:CDS	40.0%
	CAGTTGATTGATGTGCTTAC+CGG	-	chr7.1:73262951-73262970	MS.gene044961:CDS	40.0%
	GACAAGCTGCTATTCGATTT+GGG	-	chr7.1:73263080-73263099	MS.gene044961:CDS	40.0%
!	AAGGTTGGTTCCAGTTTGTT+AGG	-	chr7.1:73263036-73263055	MS.gene044961:CDS	40.0%
!!	AGGTTGGTTCCAGTTTGTTA+GGG	-	chr7.1:73263037-73263056	MS.gene044961:CDS	40.0%
	AGGGACAATCACCTACAAGA+GGG	-	chr7.1:73263056-73263075	MS.gene044961:CDS	45.0%
	CATTACCGTTGAGCGTAACA+AGG	-	chr7.1:73262995-73263014	MS.gene044961:CDS	45.0%
	CGACAAGCTGCTATTCGATT+TGG	-	chr7.1:73263079-73263098	MS.gene044961:CDS	45.0%
	GCTGAAAAATCCTTGGGAGA+AGG	-	chr7.1:73263017-73263036	MS.gene044961:CDS	45.0%
	GGTGATTGTCCCTAACAAAC+TGG	+	chr7.1:73263049-73263068	None:intergenic	45.0%
	TAGGGACAATCACCTACAAG+AGG	-	chr7.1:73263055-73263074	MS.gene044961:CDS	45.0%
	TGTTACGCTCAACGGTAATG+AGG	+	chr7.1:73262995-73263014	None:intergenic	45.0%
!!	TTCAGCCTTGTTACGCTCAA+CGG	+	chr7.1:73263003-73263022	None:intergenic	45.0%
	GATTGATGTGCTTACCGGTC+AGG	-	chr7.1:73262956-73262975	MS.gene044961:CDS	50.0%
	TACGCTCAACGGTAATGAGG+CGG	+	chr7.1:73262992-73263011	None:intergenic	50.0%
	CTGGAACCAACCTTCTCCCA+AGG	+	chr7.1:73263030-73263049	None:intergenic	55.0%
	GCGGCAATCGTATTCCTGAC+CGG	+	chr7.1:73262973-73262992	None:intergenic	55.0%
	GCAGCTTGTCGCCCTCTTGT+AGG	+	chr7.1:73263070-73263089	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	73262948	73263157	73262948	ID=MS.gene044961
chr7.1	mRNA	73262948	73263157	73262948	ID=MS.gene044961.t1;Parent=MS.gene044961
chr7.1	exon	73262948	73263157	73262948	ID=MS.gene044961.t1.exon1;Parent=MS.gene044961.t1
chr7.1	CDS	73262948	73263157	73262948	ID=cds.MS.gene044961.t1;Parent=MS.gene044961.t1

Gene Sequence	>MS.gene044961 ATGCAGTTGATTGATGTGCTTACCGGTCAGGAATACGATTGCCGCCTCATTACCGTTGAGCGTAACAAGGCTGAAAAATCCTTGGGAGAAGGTTGGTTCCAGTTTGTTAGGGACAATCACCTACAAGAGGGCGACAAGCTGCTATTCGATTTGGGTGTTCCTCCTACAATGTTATTTGTTGAGGTGGTTCGTAGAAATAATCTTGCTTGA
Protein sequence	>MS.gene044961.t1 MQLIDVLTGQEYDCRLITVERNKAEKSLGEGWFQFVRDNHLQEGDKLLFDLGVPPTMLFVEVVRRNNLA