Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044982.t1 | AES78040.1 | 96.6 | 176 | 6 | 0 | 1 | 176 | 1 | 176 | 5.90E-85 | 323.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044982.t1 | Q5QMM3 | 48.0 | 179 | 66 | 4 | 1 | 152 | 1 | 179 | 1.4e-31 | 137.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044982.t1 | G7KVS3 | 96.6 | 176 | 6 | 0 | 1 | 176 | 1 | 176 | 4.2e-85 | 323.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene044982.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044982.t1 | MTR_7g023810 | 96.591 | 176 | 6 | 0 | 1 | 176 | 1 | 176 | 3.14e-125 | 350 |
| MS.gene044982.t1 | MTR_1g115315 | 61.290 | 186 | 50 | 5 | 1 | 165 | 5 | 189 | 7.01e-67 | 205 |
| MS.gene044982.t1 | MTR_1g115315 | 61.290 | 186 | 50 | 5 | 1 | 165 | 5 | 189 | 1.19e-66 | 204 |
| MS.gene044982.t1 | MTR_3g115620 | 45.026 | 191 | 79 | 4 | 9 | 176 | 7 | 194 | 8.05e-46 | 150 |
| MS.gene044982.t1 | MTR_4g084550 | 33.071 | 127 | 73 | 3 | 36 | 150 | 25 | 151 | 5.97e-14 | 66.6 |
| MS.gene044982.t1 | MTR_8g107210 | 48.387 | 62 | 32 | 0 | 68 | 129 | 96 | 157 | 3.06e-13 | 67.0 |
| MS.gene044982.t1 | MTR_2g015000 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 9.72e-12 | 62.8 |
| MS.gene044982.t1 | MTR_4g088080 | 43.333 | 60 | 34 | 0 | 66 | 125 | 38 | 97 | 3.23e-11 | 60.8 |
| MS.gene044982.t1 | MTR_4g063735 | 45.161 | 62 | 34 | 0 | 68 | 129 | 25 | 86 | 7.74e-11 | 59.3 |
| MS.gene044982.t1 | MTR_1g019130 | 31.304 | 115 | 72 | 2 | 53 | 164 | 79 | 189 | 9.27e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044982.t1 | AT4G35550 | 57.237 | 152 | 45 | 2 | 17 | 148 | 33 | 184 | 4.81e-47 | 154 |
| MS.gene044982.t1 | AT1G20700 | 47.333 | 150 | 56 | 4 | 15 | 141 | 24 | 173 | 4.45e-36 | 124 |
| MS.gene044982.t1 | AT1G20700 | 47.333 | 150 | 56 | 4 | 15 | 141 | 24 | 173 | 5.03e-36 | 124 |
| MS.gene044982.t1 | AT1G20710 | 45.113 | 133 | 53 | 2 | 15 | 128 | 8 | 139 | 1.31e-25 | 97.8 |
| MS.gene044982.t1 | AT1G20700 | 54.321 | 81 | 32 | 1 | 66 | 141 | 31 | 111 | 4.28e-23 | 89.7 |
| MS.gene044982.t1 | AT3G18010 | 45.161 | 62 | 34 | 0 | 68 | 129 | 76 | 137 | 3.22e-12 | 63.9 |
| MS.gene044982.t1 | AT2G33880 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 1.46e-11 | 62.0 |
| MS.gene044982.t1 | AT2G33880 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 1.67e-11 | 62.0 |
| MS.gene044982.t1 | AT5G45980 | 41.935 | 62 | 36 | 0 | 66 | 127 | 53 | 114 | 3.56e-11 | 60.8 |
Find 34 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGACCAGTGTGTATAAT+TGG | 0.216618 | 7.1:+73603237 | MS.gene044982:CDS |
| ACCTGTGCAACTGCAAAGTT+TGG | 0.336692 | 7.1:+73603127 | MS.gene044982:CDS |
| TGTATAATTGGTTTCAGAAT+AGG | 0.361284 | 7.1:+73603249 | MS.gene044982:CDS |
| GAGATCTTGTTGAGAAGATA+AGG | 0.380614 | 7.1:-73602874 | None:intergenic |
| TCCTAGTAAGGAAAAGATAA+AGG | 0.397905 | 7.1:+73603178 | MS.gene044982:CDS |
| TACACACTGGTCTCTGAAAT+CGG | 0.399480 | 7.1:-73603230 | None:intergenic |
| TTTGTGCAGCCAGTACTTAG+AGG | 0.411680 | 7.1:+73603394 | MS.gene044982:CDS |
| CTCTGAAATCGGCCCGTGCT+TGG | 0.426359 | 7.1:-73603219 | None:intergenic |
| TCAACCTCAGTCTCTACTTC+CGG | 0.438371 | 7.1:-73603317 | None:intergenic |
| AAATAATGTTAATGATGAAC+CGG | 0.459463 | 7.1:+73603298 | MS.gene044982:CDS |
| GACTGAGATGCAACGATCTC+CGG | 0.461176 | 7.1:-73603362 | None:intergenic |
| CGAATATTTGAAGCTGAGAC+GGG | 0.462716 | 7.1:+73603152 | MS.gene044982:CDS |
| ATGAACAAGTCGAAACTCTT+CGG | 0.463263 | 7.1:+73602796 | MS.gene044982:CDS |
| TCGTAAACTGTCATCTGATT+CGG | 0.469903 | 7.1:-73603426 | None:intergenic |
| CACTTGCATCATTCCAACAT+TGG | 0.480071 | 7.1:-73602760 | None:intergenic |
| TCCAAACTTTGCAGTTGCAC+AGG | 0.484501 | 7.1:-73603128 | None:intergenic |
| CTACTAGGCTGAGGCAGCGT+TGG | 0.489931 | 7.1:+73603096 | MS.gene044982:CDS |
| CAATGATAAGAAAACTGAAC+CGG | 0.495461 | 7.1:+73603343 | MS.gene044982:CDS |
| AAGAGTTTCGACTTGTTCAT+CGG | 0.503160 | 7.1:-73602793 | None:intergenic |
| GGTTTCAGAATAGGCGTGCT+AGG | 0.518890 | 7.1:+73603258 | MS.gene044982:CDS |
| AATTCAAGTCCTCTAAGTAC+TGG | 0.519200 | 7.1:-73603403 | None:intergenic |
| GCGAATATTTGAAGCTGAGA+CGG | 0.525730 | 7.1:+73603151 | MS.gene044982:CDS |
| TGAGACGGGGACTCCTAGTA+AGG | 0.526200 | 7.1:+73603166 | MS.gene044982:CDS |
| CTTTGCAGTTGCACAGGTGT+TGG | 0.529341 | 7.1:-73603122 | None:intergenic |
| TTTAATCAGAACGCCAATGT+TGG | 0.533338 | 7.1:+73602747 | MS.gene044982:CDS |
| GAATAGGCGTGCTAGGTCAA+AGG | 0.534265 | 7.1:+73603265 | MS.gene044982:CDS |
| AATAGGCGTGCTAGGTCAAA+GGG | 0.534387 | 7.1:+73603266 | MS.gene044982:CDS |
| CAACAAGATCTCACTCTCAC+TGG | 0.535564 | 7.1:+73602885 | MS.gene044982:CDS |
| ACCGCTGATTTAACCAAGCA+CGG | 0.547494 | 7.1:+73603206 | MS.gene044982:CDS |
| CCCGTGCTTGGTTAAATCAG+CGG | 0.597671 | 7.1:-73603207 | None:intergenic |
| CCGCTGATTTAACCAAGCAC+GGG | 0.638814 | 7.1:+73603207 | MS.gene044982:CDS |
| CTGAAACCAATTATACACAC+TGG | 0.642488 | 7.1:-73603243 | None:intergenic |
| GAATATTTGAAGCTGAGACG+GGG | 0.658394 | 7.1:+73603153 | MS.gene044982:CDS |
| TGAACCGGAAGTAGAGACTG+AGG | 0.698771 | 7.1:+73603313 | MS.gene044982:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTAATGATGATTTTT+AGG | + | chr7.1:73603071-73603090 | MS.gene044982:intron | 15.0% |
| !!! | ACATTTAATGATGATTTTTA+GGG | + | chr7.1:73603072-73603091 | MS.gene044982:intron | 15.0% |
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr7.1:73603298-73603317 | MS.gene044982:CDS | 20.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr7.1:73603249-73603268 | MS.gene044982:CDS | 25.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr7.1:73603343-73603362 | MS.gene044982:CDS | 30.0% | |
| TCCTAGTAAGGAAAAGATAA+AGG | + | chr7.1:73603178-73603197 | MS.gene044982:CDS | 30.0% | |
| ! | TCCTTTATCTTTTCCTTACT+AGG | - | chr7.1:73603182-73603201 | None:intergenic | 30.0% |
| AATTCAAGTCCTCTAAGTAC+TGG | - | chr7.1:73603406-73603425 | None:intergenic | 35.0% | |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr7.1:73602796-73602815 | MS.gene044982:CDS | 35.0% | |
| CTGAAACCAATTATACACAC+TGG | - | chr7.1:73603246-73603265 | None:intergenic | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr7.1:73602877-73602896 | None:intergenic | 35.0% | |
| GTTCAGTTACTCATTCTCAA+CGG | + | chr7.1:73602929-73602948 | MS.gene044982:intron | 35.0% | |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr7.1:73602986-73603005 | MS.gene044982:intron | 35.0% | |
| TTTAATCAGAACGCCAATGT+TGG | + | chr7.1:73602747-73602766 | MS.gene044982:CDS | 35.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr7.1:73602796-73602815 | None:intergenic | 35.0% |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr7.1:73602978-73602997 | None:intergenic | 35.0% |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr7.1:73603345-73603364 | None:intergenic | 35.0% |
| !!! | GATGATTTTTAGGGTCTACT+AGG | + | chr7.1:73603081-73603100 | MS.gene044982:intron | 35.0% |
| AACCAAACCGTACTAAAGAC+CGG | - | chr7.1:73602996-73603015 | None:intergenic | 40.0% | |
| CACTTGCATCATTCCAACAT+TGG | - | chr7.1:73602763-73602782 | None:intergenic | 40.0% | |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr7.1:73603237-73603256 | MS.gene044982:CDS | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr7.1:73602974-73602993 | MS.gene044982:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr7.1:73602977-73602996 | None:intergenic | 40.0% | |
| TCAACGGTATCACAGTTAAC+CGG | + | chr7.1:73602945-73602964 | MS.gene044982:intron | 40.0% | |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr7.1:73603152-73603171 | MS.gene044982:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr7.1:73603153-73603172 | MS.gene044982:CDS | 40.0% |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr7.1:73603151-73603170 | MS.gene044982:CDS | 40.0% |
| !! | TACACACTGGTCTCTGAAAT+CGG | - | chr7.1:73603233-73603252 | None:intergenic | 40.0% |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr7.1:73602991-73603010 | MS.gene044982:intron | 45.0% | |
| AATAGGCGTGCTAGGTCAAA+GGG | + | chr7.1:73603266-73603285 | MS.gene044982:CDS | 45.0% | |
| ACCTGTGCAACTGCAAAGTT+TGG | + | chr7.1:73603127-73603146 | MS.gene044982:CDS | 45.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr7.1:73602885-73602904 | MS.gene044982:CDS | 45.0% | |
| CAACGGTATCACAGTTAACC+GGG | + | chr7.1:73602946-73602965 | MS.gene044982:intron | 45.0% | |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr7.1:73603320-73603339 | None:intergenic | 45.0% | |
| TCCAAACTTTGCAGTTGCAC+AGG | - | chr7.1:73603131-73603150 | None:intergenic | 45.0% | |
| TTTGTGCAGCCAGTACTTAG+AGG | + | chr7.1:73603394-73603413 | MS.gene044982:CDS | 45.0% | |
| !! | ACCGCTGATTTAACCAAGCA+CGG | + | chr7.1:73603206-73603225 | MS.gene044982:CDS | 45.0% |
| !!! | TTTTAGGGTCTACTAGGCTG+AGG | + | chr7.1:73603087-73603106 | MS.gene044982:intron | 45.0% |
| CCCGTGCTTGGTTAAATCAG+CGG | - | chr7.1:73603210-73603229 | None:intergenic | 50.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr7.1:73603125-73603144 | None:intergenic | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr7.1:73603265-73603284 | MS.gene044982:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr7.1:73603365-73603384 | None:intergenic | 50.0% | |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr7.1:73603258-73603277 | MS.gene044982:CDS | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr7.1:73603313-73603332 | MS.gene044982:CDS | 50.0% | |
| !! | CCGCTGATTTAACCAAGCAC+GGG | + | chr7.1:73603207-73603226 | MS.gene044982:CDS | 50.0% |
| ATATTGAACCGGGCTCCACC+CGG | - | chr7.1:73602967-73602986 | None:intergenic | 55.0% | |
| CGGTATCACAGTTAACCGGG+TGG | + | chr7.1:73602949-73602968 | MS.gene044982:intron | 55.0% | |
| TGAGACGGGGACTCCTAGTA+AGG | + | chr7.1:73603166-73603185 | MS.gene044982:CDS | 55.0% | |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr7.1:73602956-73602975 | MS.gene044982:intron | 60.0% | |
| CTACTAGGCTGAGGCAGCGT+TGG | + | chr7.1:73603096-73603115 | MS.gene044982:CDS | 60.0% | |
| !! | CTCTGAAATCGGCCCGTGCT+TGG | - | chr7.1:73603222-73603241 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 73602726 | 73603440 | 73602726 | ID=MS.gene044982 |
| chr7.1 | mRNA | 73602726 | 73603440 | 73602726 | ID=MS.gene044982.t1;Parent=MS.gene044982 |
| chr7.1 | exon | 73602726 | 73602906 | 73602726 | ID=MS.gene044982.t1.exon1;Parent=MS.gene044982.t1 |
| chr7.1 | CDS | 73602726 | 73602906 | 73602726 | ID=cds.MS.gene044982.t1;Parent=MS.gene044982.t1 |
| chr7.1 | exon | 73603093 | 73603440 | 73603093 | ID=MS.gene044982.t1.exon2;Parent=MS.gene044982.t1 |
| chr7.1 | CDS | 73603093 | 73603440 | 73603093 | ID=cds.MS.gene044982.t1;Parent=MS.gene044982.t1 |
| Gene Sequence |
| Protein sequence |
