Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045016.t1 | PNX89523.1 | 39.6 | 338 | 188 | 5 | 17 | 352 | 11 | 334 | 1.50E-10 | 77.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045016.t1 | A0A2K3MFE8 | 39.6 | 338 | 188 | 5 | 17 | 352 | 11 | 334 | 1.1e-10 | 77.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene045016.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 161 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTTAAAAAAGAACAC+AGG | - | 37914:3730-3749 | MS.gene045016:CDS | 15.0% |
| !!! | AAATGATAACAATTTAAAAC+AGG | + | 37914:4672-4691 | None:intergenic | 15.0% |
| !!! | AATTACTGTTATATAGTTTT+TGG | - | 37914:4631-4650 | MS.gene045016:intron | 15.0% |
| ! | AAATCAAAGAAATTATGTGG+AGG | + | 37914:4378-4397 | None:intergenic | 25.0% |
| ! | AGTACACAAAATCTTAGATA+CGG | + | 37914:3390-3409 | None:intergenic | 25.0% |
| ! | GCAAAATCAAAGAAATTATG+TGG | + | 37914:4381-4400 | None:intergenic | 25.0% |
| !!! | CATTTGTTGTTACATGTTTA+AGG | - | 37914:3277-3296 | MS.gene045016:CDS | 25.0% |
| AAATATATTGCAGAGTGACA+AGG | + | 37914:3473-3492 | None:intergenic | 30.0% | |
| AGTACACATTATCGTAGATA+CGG | + | 37914:3577-3596 | None:intergenic | 30.0% | |
| ATTACAGAGTGACAAAAAAC+TGG | + | 37914:3621-3640 | None:intergenic | 30.0% | |
| TATAATTCATACTCCTCCAA+GGG | + | 37914:4150-4169 | None:intergenic | 30.0% | |
| TTACAGAGTGACAAAAAACT+GGG | + | 37914:3620-3639 | None:intergenic | 30.0% | |
| TTGTTACTAAACTTTGAGTG+TGG | - | 37914:3174-3193 | MS.gene045016:CDS | 30.0% | |
| AAAATCTACCGGATACCAAA+AGG | + | 37914:3248-3267 | None:intergenic | 35.0% | |
| AAATCTACCGGATACCAAAA+GGG | + | 37914:3247-3266 | None:intergenic | 35.0% | |
| AAGTGGTTGTTGAAGTATGA+TGG | + | 37914:3850-3869 | None:intergenic | 35.0% | |
| ACCCATATGTTATGTATGCT+AGG | - | 37914:4920-4939 | MS.gene045016:CDS | 35.0% | |
| ACTCCTTAGCCTATAGAAAA+AGG | + | 37914:3959-3978 | None:intergenic | 35.0% | |
| ATACTGCGTCTAAGTTACAA+CGG | - | 37914:4012-4031 | MS.gene045016:CDS | 35.0% | |
| ATCTTCATCGTTGATATCCA+TGG | + | 37914:4409-4428 | None:intergenic | 35.0% | |
| ATTACAGAGTGACAACAAAC+AGG | + | 37914:3434-3453 | None:intergenic | 35.0% | |
| CCCATATGTTATGTATGCTA+GGG | - | 37914:4921-4940 | MS.gene045016:CDS | 35.0% | |
| CCCTAGCATACATAACATAT+GGG | + | 37914:4924-4943 | None:intergenic | 35.0% | |
| GGTAACAATGGTGAAAACAT+GGG | - | 37914:4489-4508 | MS.gene045016:intron | 35.0% | |
| GTATAATTCATACTCCTCCA+AGG | + | 37914:4151-4170 | None:intergenic | 35.0% | |
| TCAAAGAAATTATGTGGAGG+CGG | + | 37914:4375-4394 | None:intergenic | 35.0% | |
| TCATACTTCAACAACCACTT+TGG | - | 37914:3850-3869 | MS.gene045016:CDS | 35.0% | |
| TCCCTAGCATACATAACATA+TGG | + | 37914:4925-4944 | None:intergenic | 35.0% | |
| TTCTTGTGTGTGCATAATAG+TGG | + | 37914:3366-3385 | None:intergenic | 35.0% | |
| TTGATTGGCATCATGAACTT+CGG | + | 37914:4322-4341 | None:intergenic | 35.0% | |
| TTTACAGAGTGACATCAAAC+TGG | + | 37914:3659-3678 | None:intergenic | 35.0% | |
| ! | GAAGTTGTCACTTGTAATGT+AGG | - | 37914:3810-3829 | MS.gene045016:CDS | 35.0% |
| ! | TTATTTTCCTGTCTCCCTTT+TGG | - | 37914:3230-3249 | MS.gene045016:CDS | 35.0% |
| AACATCAATGCCAAGATCGA+TGG | + | 37914:4214-4233 | None:intergenic | 40.0% | |
| AACCAACAACAAGGCAAACA+TGG | + | 37914:3499-3518 | None:intergenic | 40.0% | |
| AACGATGAAGATGTGGAAGA+TGG | - | 37914:4417-4436 | MS.gene045016:intron | 40.0% | |
| ACTCGTGCAACAAATCTTCT+TGG | + | 37914:4709-4728 | None:intergenic | 40.0% | |
| ATCCTACTTACAGATGCCAT+GGG | - | 37914:4754-4773 | MS.gene045016:intron | 40.0% | |
| ATGTTTGCCTTCTTGTTGGT+TGG | - | 37914:3309-3328 | MS.gene045016:CDS | 40.0% | |
| GACTATGTTTGCCTTCTTGT+TGG | - | 37914:3305-3324 | MS.gene045016:CDS | 40.0% | |
| GCAGCATCAAAGAAGTTATG+CGG | + | 37914:4456-4475 | None:intergenic | 40.0% | |
| GGATATCAACGATGAAGATG+TGG | - | 37914:4410-4429 | MS.gene045016:intron | 40.0% | |
| GGGTAACAATGGTGAAAACA+TGG | - | 37914:4488-4507 | MS.gene045016:intron | 40.0% | |
| GTCACTTGTAATGTAGGATG+AGG | - | 37914:3816-3835 | MS.gene045016:CDS | 40.0% | |
| GTGCAACAAATCTTCTTGGT+AGG | + | 37914:4705-4724 | None:intergenic | 40.0% | |
| GTGGGAATGTGGCTAATAAT+GGG | - | 37914:4517-4536 | MS.gene045016:intron | 40.0% | |
| TAAGACACACTTACCTTGTC+TGG | + | 37914:3140-3159 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAACA+AGG | + | 37914:3508-3527 | None:intergenic | 40.0% | |
| TCATCATCCAACCAACAAGA+AGG | + | 37914:3319-3338 | None:intergenic | 40.0% | |
| TGAAGCCATGCAAAATCTAC+CGG | + | 37914:3259-3278 | None:intergenic | 40.0% | |
| TGATTGGCATCATGAACTTC+GGG | + | 37914:4321-4340 | None:intergenic | 40.0% | |
| TGTCAGACTTGCAGAATACA+TGG | - | 37914:4557-4576 | MS.gene045016:intron | 40.0% | |
| TTATGTATGCTAGGGAAGCT+CGG | - | 37914:4929-4948 | MS.gene045016:CDS | 40.0% | |
| ! | GGACCTTTTTCTATAGGCTA+AGG | - | 37914:3953-3972 | MS.gene045016:CDS | 40.0% |
| ! | GGTGTTTTCATAGGAAGTGA+GGG | - | 37914:3925-3944 | MS.gene045016:CDS | 40.0% |
| ! | GTGTTTTCATAGGAAGTGAG+GGG | - | 37914:3926-3945 | MS.gene045016:CDS | 40.0% |
| ! | GTTTTCACCATTGTTACCCA+TGG | + | 37914:4487-4506 | None:intergenic | 40.0% |
| ! | TGGTGTTTTCATAGGAAGTG+AGG | - | 37914:3924-3943 | MS.gene045016:CDS | 40.0% |
| !! | AATTATGTGGAGGCGGATTT+TGG | + | 37914:4368-4387 | None:intergenic | 40.0% |
| !! | AGAGATTTGTTACGGAGAAC+CGG | - | 37914:4854-4873 | MS.gene045016:intron | 40.0% |
| !! | AGATTTGTTGCACGAGTGTT+TGG | - | 37914:4712-4731 | MS.gene045016:intron | 40.0% |
| !! | ATGTTTGCCTTGTTGTTGGT+TGG | - | 37914:3498-3517 | MS.gene045016:CDS | 40.0% |
| !! | ATTATGTGGAGGCGGATTTT+GGG | + | 37914:4367-4386 | None:intergenic | 40.0% |
| !! | GATTTGTTGCACGAGTGTTT+GGG | - | 37914:4713-4732 | MS.gene045016:intron | 40.0% |
| !!! | TCGAGGTTTGGTGTTTTCAT+AGG | - | 37914:3916-3935 | MS.gene045016:CDS | 40.0% |
| AATGGTGAAAACATGGGTGC+AGG | - | 37914:4495-4514 | MS.gene045016:intron | 45.0% | |
| AGACGCAGTATGAGACCTAA+CGG | + | 37914:4003-4022 | None:intergenic | 45.0% | |
| ATCGTCGTCGTTGTCTTCAT+CGG | + | 37914:4187-4206 | None:intergenic | 45.0% | |
| ATGAACTACGACACAGAGTC+TGG | - | 37914:3875-3894 | MS.gene045016:CDS | 45.0% | |
| CACAACAATCGATCCAGACA+AGG | - | 37914:3124-3143 | MS.gene045016:CDS | 45.0% | |
| CATCCTACTTACAGATGCCA+TGG | - | 37914:4753-4772 | MS.gene045016:intron | 45.0% | |
| CATGGCATCTGTAAGTAGGA+TGG | + | 37914:4755-4774 | None:intergenic | 45.0% | |
| CATGGTGAAGACTAGCAATC+TGG | - | 37914:4575-4594 | MS.gene045016:intron | 45.0% | |
| CGTCTAAGTTACAACGGACA+GGG | - | 37914:4018-4037 | MS.gene045016:CDS | 45.0% | |
| CTATAGGCTAAGGAGTTTGC+TGG | - | 37914:3963-3982 | MS.gene045016:CDS | 45.0% | |
| GAACTTCCTGAATGTAAGCG+CGG | + | 37914:3102-3121 | None:intergenic | 45.0% | |
| GACGATGATTCCATCGATCT+TGG | - | 37914:4201-4220 | MS.gene045016:CDS | 45.0% | |
| GCATCAAAGAAGTTATGCGG+AGG | + | 37914:4453-4472 | None:intergenic | 45.0% | |
| GCATCTGTAAGTAGGATGGT+GGG | + | 37914:4751-4770 | None:intergenic | 45.0% | |
| GGCATTGTGTGGTTGTTGAT+TGG | + | 37914:4337-4356 | None:intergenic | 45.0% | |
| GGTGGGAATGTGGCTAATAA+TGG | - | 37914:4516-4535 | MS.gene045016:intron | 45.0% | |
| GTCGTAGTTCATCACCAAAG+TGG | + | 37914:3867-3886 | None:intergenic | 45.0% | |
| GTCTAAGTTACAACGGACAG+GGG | - | 37914:4019-4038 | MS.gene045016:CDS | 45.0% | |
| GTCTGGTTAGAAGACACAAG+AGG | - | 37914:3892-3911 | MS.gene045016:CDS | 45.0% | |
| GTGAAGACTAGCAATCTGGT+AGG | - | 37914:4579-4598 | MS.gene045016:intron | 45.0% | |
| TAGAAGACACAAGAGGTTCG+AGG | - | 37914:3899-3918 | MS.gene045016:CDS | 45.0% | |
| ! | GACCATGTTTGCCTTGTTGT+TGG | - | 37914:3494-3513 | MS.gene045016:CDS | 45.0% |
| ! | GGTATCCGGTAGATTTTGCA+TGG | - | 37914:3251-3270 | MS.gene045016:CDS | 45.0% |
| ! | TGTGGCTAATAATGGGAGAG+TGG | - | 37914:4524-4543 | MS.gene045016:intron | 45.0% |
| !! | CGGGTGGAAGAGATTTGTTA+CGG | - | 37914:4846-4865 | MS.gene045016:intron | 45.0% |
| !! | CTTTGATTTTGCTGCTGCCA+TGG | - | 37914:4389-4408 | MS.gene045016:intron | 45.0% |
| !! | TGGCAGTTGGGAAAAGTGTT+AGG | - | 37914:4878-4897 | MS.gene045016:intron | 45.0% |
| ACATCAACATCCGCTGGATC+CGG | + | 37914:4276-4295 | None:intergenic | 50.0% | |
| ACTCGACATGACCGAGCTAA+CGG | - | 37914:4065-4084 | MS.gene045016:CDS | 50.0% | |
| ACTTTCCGTGCAGGATCAAG+TGG | - | 37914:4782-4801 | MS.gene045016:intron | 50.0% | |
| AGCCCATGGCATCTGTAAGT+AGG | + | 37914:4759-4778 | None:intergenic | 50.0% | |
| AGGATCAAGTGGAAGGACAG+AGG | - | 37914:4793-4812 | MS.gene045016:intron | 50.0% | |
| ATCTCGGCAGCAATCAGGAA+AGG | + | 37914:4120-4139 | None:intergenic | 50.0% | |
| CTCCAAGGGATCCAGTATCT+CGG | + | 37914:4136-4155 | None:intergenic | 50.0% | |
| GACACAAGAGGTTCGAGGTT+TGG | - | 37914:3904-3923 | MS.gene045016:CDS | 50.0% | |
| GCATCATGAACTTCGGGATG+AGG | + | 37914:4315-4334 | None:intergenic | 50.0% | |
| GCGTCTAAGTTACAACGGAC+AGG | - | 37914:4017-4036 | MS.gene045016:CDS | 50.0% | |
| GCTGTTGCCATGGGTAACAA+TGG | - | 37914:4477-4496 | MS.gene045016:intron | 50.0% | |
| GGCATCTGTAAGTAGGATGG+TGG | + | 37914:4752-4771 | None:intergenic | 50.0% | |
| GTGAAAACATGGGTGCAGGT+GGG | - | 37914:4499-4518 | MS.gene045016:intron | 50.0% | |
| GTTGTCTTCATCGGATGACG+AGG | + | 37914:4178-4197 | None:intergenic | 50.0% | |
| TCATGAACTTCGGGATGAGG+AGG | + | 37914:4312-4331 | None:intergenic | 50.0% | |
| TCCTTCCACTTGATCCTGCA+CGG | + | 37914:4790-4809 | None:intergenic | 50.0% | |
| TCGGGATGAGGAGGAGAATT+GGG | + | 37914:4303-4322 | None:intergenic | 50.0% | |
| TTCAACTCCTGTGGCAGAAC+CGG | - | 37914:4254-4273 | MS.gene045016:intron | 50.0% | |
| TTCGGGATGAGGAGGAGAAT+TGG | + | 37914:4304-4323 | None:intergenic | 50.0% | |
| ! | ATTTGTTACGGAGAACCGGC+TGG | - | 37914:4858-4877 | MS.gene045016:intron | 50.0% |
| ! | CCTGTCTCCCTTTTGGTATC+CGG | - | 37914:3237-3256 | MS.gene045016:CDS | 50.0% |
| ! | GGACGCGGACCTTTTTCTAT+AGG | - | 37914:3947-3966 | MS.gene045016:CDS | 50.0% |
| !! | AGGCGGATTTTGGGCATCAT+CGG | + | 37914:4358-4377 | None:intergenic | 50.0% |
| !! | TCTGTAAGTAGGATGGTGGG+CGG | + | 37914:4748-4767 | None:intergenic | 50.0% |
| CCAGTATCTCGGCAGCAATC+AGG | + | 37914:4125-4144 | None:intergenic | 55.0% | |
| CCGGATACCAAAAGGGAGAC+AGG | + | 37914:3240-3259 | None:intergenic | 55.0% | |
| CCTCTCACGAACCGTTAGCT+CGG | + | 37914:4079-4098 | None:intergenic | 55.0% | |
| CCTGATTGCTGCCGAGATAC+TGG | - | 37914:4122-4141 | MS.gene045016:CDS | 55.0% | |
| CGAGATACTGGATCCCTTGG+AGG | - | 37914:4134-4153 | MS.gene045016:CDS | 55.0% | |
| CGGCAGCAATCAGGAAAGGA+GGG | + | 37914:4116-4135 | None:intergenic | 55.0% | |
| GAAATGCCGCTGGAGGAAGA+TGG | + | 37914:4819-4838 | None:intergenic | 55.0% | |
| GATGCTGCTGCTGTTGCCAT+GGG | - | 37914:4468-4487 | MS.gene045016:intron | 55.0% | |
| GCACGGAAAGTCAAAGCCCA+TGG | + | 37914:4773-4792 | None:intergenic | 55.0% | |
| GCAGTATGAGACCTAACGGC+TGG | + | 37914:3999-4018 | None:intergenic | 55.0% | |
| GGACAGGGGCTGTTCTACAT+GGG | - | 37914:4033-4052 | MS.gene045016:CDS | 55.0% | |
| GGTGAAAACATGGGTGCAGG+TGG | - | 37914:4498-4517 | MS.gene045016:intron | 55.0% | |
| GGTGCAACATCAACATCCGC+TGG | + | 37914:4282-4301 | None:intergenic | 55.0% | |
| GGTTCTGCCACAGGAGTTGA+AGG | + | 37914:4255-4274 | None:intergenic | 55.0% | |
| GTATGAGACCTAACGGCTGG+TGG | + | 37914:3996-4015 | None:intergenic | 55.0% | |
| GTATGCTAGGGAAGCTCGGA+TGG | - | 37914:4933-4952 | MS.gene045016:CDS | 55.0% | |
| TCATAGGAAGTGAGGGGACG+CGG | - | 37914:3932-3951 | MS.gene045016:CDS | 55.0% | |
| TCCGTGCAGGATCAAGTGGA+AGG | - | 37914:4786-4805 | MS.gene045016:intron | 55.0% | |
| TCCTCCAGCGGCATTTCTCA+GGG | - | 37914:4822-4841 | MS.gene045016:intron | 55.0% | |
| TCGGCAGCAATCAGGAAAGG+AGG | + | 37914:4117-4136 | None:intergenic | 55.0% | |
| TCTGCCACAGGAGTTGAAGG+AGG | + | 37914:4252-4271 | None:intergenic | 55.0% | |
| TGATGCTGCTGCTGTTGCCA+TGG | - | 37914:4467-4486 | MS.gene045016:intron | 55.0% | |
| TGCACCTCCTTCAACTCCTG+TGG | - | 37914:4245-4264 | MS.gene045016:intron | 55.0% | |
| TGCCGAGATACTGGATCCCT+TGG | - | 37914:4131-4150 | MS.gene045016:CDS | 55.0% | |
| TGGGCATCATCGGCATTGTG+TGG | + | 37914:4348-4367 | None:intergenic | 55.0% | |
| TTCCTCCAGCGGCATTTCTC+AGG | - | 37914:4821-4840 | MS.gene045016:intron | 55.0% | |
| ! | ACAGGAGTTGAAGGAGGTGC+AGG | + | 37914:4246-4265 | None:intergenic | 55.0% |
| ! | CACTTTTCCCAACTGCCAGC+CGG | + | 37914:4876-4895 | None:intergenic | 55.0% |
| ! | TGGGCTTTGACTTTCCGTGC+AGG | - | 37914:4773-4792 | MS.gene045016:intron | 55.0% |
| CAGAGGCCATCTTCCTCCAG+CGG | - | 37914:4810-4829 | MS.gene045016:intron | 60.0% | |
| CATGGGTGCAGGTGGGAATG+TGG | - | 37914:4506-4525 | MS.gene045016:intron | 60.0% | |
| CCGAGCTAACGGTTCGTGAG+AGG | - | 37914:4076-4095 | MS.gene045016:CDS | 60.0% | |
| CCTCCAGCGGCATTTCTCAG+GGG | - | 37914:4823-4842 | MS.gene045016:intron | 60.0% | |
| CGGACAGGGGCTGTTCTACA+TGG | - | 37914:4032-4051 | MS.gene045016:CDS | 60.0% | |
| GAGCACTTCCACCAGCCGTT+AGG | - | 37914:3985-4004 | MS.gene045016:CDS | 60.0% | |
| TGTGGCAGAACCGGATCCAG+CGG | - | 37914:4263-4282 | MS.gene045016:intron | 60.0% | |
| ! | GGAGTTGAAGGAGGTGCAGG+AGG | + | 37914:4243-4262 | None:intergenic | 60.0% |
| ACGGAGAACCGGCTGGCAGT+TGG | - | 37914:4865-4884 | MS.gene045016:intron | 65.0% | |
| CAGCGGCATTTCTCAGGGGC+GGG | - | 37914:4827-4846 | MS.gene045016:intron | 65.0% | |
| CCAGCGGCATTTCTCAGGGG+CGG | - | 37914:4826-4845 | MS.gene045016:intron | 65.0% | |
| CCCCTGAGAAATGCCGCTGG+AGG | + | 37914:4826-4845 | None:intergenic | 65.0% | |
| CGGAGAACCGGCTGGCAGTT+GGG | - | 37914:4866-4885 | MS.gene045016:intron | 65.0% | |
| GCTGGATCCGGTTCTGCCAC+AGG | + | 37914:4264-4283 | None:intergenic | 65.0% | |
| CCGCCCCTGAGAAATGCCGC+TGG | + | 37914:4829-4848 | None:intergenic | 70.0% | |
| CGGCATTTCTCAGGGGCGGG+TGG | - | 37914:4830-4849 | MS.gene045016:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37914 | gene | 3095 | 4963 | 3095 | ID=MS.gene045016 |
| 37914 | mRNA | 3095 | 4963 | 3095 | ID=MS.gene045016.t1;Parent=MS.gene045016 |
| 37914 | exon | 4913 | 4963 | 4913 | ID=MS.gene045016.t1.exon1;Parent=MS.gene045016.t1 |
| 37914 | CDS | 4913 | 4963 | 4913 | ID=cds.MS.gene045016.t1;Parent=MS.gene045016.t1 |
| 37914 | exon | 3462 | 4226 | 3462 | ID=MS.gene045016.t1.exon2;Parent=MS.gene045016.t1 |
| 37914 | CDS | 3462 | 4226 | 3462 | ID=cds.MS.gene045016.t1;Parent=MS.gene045016.t1 |
| 37914 | exon | 3095 | 3358 | 3095 | ID=MS.gene045016.t1.exon3;Parent=MS.gene045016.t1 |
| 37914 | CDS | 3095 | 3358 | 3095 | ID=cds.MS.gene045016.t1;Parent=MS.gene045016.t1 |
| Gene Sequence |
| Protein sequence |
