AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045148

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045148.t1 MTR_3g086790 98.013 302 5 1 1 301 1 302 0.0 606
MS.gene045148.t1 MTR_8g089895 58.716 327 93 11 1 301 1 311 6.65e-116 336
MS.gene045148.t1 MTR_3g080100 41.297 293 133 7 1 292 1 255 4.13e-49 164
MS.gene045148.t1 MTR_3g080100 43.083 253 107 5 40 292 1 216 1.37e-46 156
MS.gene045148.t1 MTR_5g038280 56.452 124 50 1 45 164 69 192 2.15e-38 138
MS.gene045148.t1 MTR_8g469430 56.557 122 49 1 47 168 81 198 2.87e-38 137
MS.gene045148.t1 MTR_8g468210 50.993 151 61 4 25 165 33 180 4.86e-38 136
MS.gene045148.t1 MTR_5g039000 56.667 120 49 1 46 165 64 180 2.14e-36 132
MS.gene045148.t1 MTR_4g107650 58.559 111 46 0 50 160 86 196 4.18e-36 132
MS.gene045148.t1 MTR_6g007647 61.321 106 41 0 58 163 32 137 4.45e-36 130
MS.gene045148.t1 MTR_6g007647 61.321 106 41 0 58 163 66 171 1.09e-35 130
MS.gene045148.t1 MTR_6g011610 48.905 137 62 2 26 154 36 172 9.30e-35 127
MS.gene045148.t1 MTR_6g011610 60.825 97 38 0 58 154 95 191 1.71e-34 127
MS.gene045148.t1 MTR_7g010020 58.763 97 40 0 58 154 79 175 2.84e-29 112
MS.gene045148.t1 MTR_7g103340 40.571 175 90 4 35 209 41 201 1.37e-28 110
MS.gene045148.t1 MTR_8g026960 36.702 188 106 5 66 241 41 227 1.03e-25 102
MS.gene045148.t1 MTR_1g061660 37.791 172 89 4 58 220 58 220 2.01e-25 101
MS.gene045148.t1 MTR_3g092150 50.549 91 45 0 61 151 40 130 1.21e-24 99.8
MS.gene045148.t1 MTR_5g019650 49.091 110 56 0 51 160 45 154 1.68e-23 96.3
MS.gene045148.t1 MTR_5g019680 58.537 82 34 0 51 132 45 126 2.15e-23 95.5
MS.gene045148.t1 MTR_4g100550 41.406 128 67 2 51 178 112 231 3.42e-18 83.2
MS.gene045148.t1 MTR_1g017090 42.500 120 62 1 59 178 130 242 3.49e-17 80.5
MS.gene045148.t1 MTR_2g061030 36.879 141 78 3 56 195 197 327 1.03e-16 79.7
MS.gene045148.t1 MTR_4g097600 41.085 129 65 3 51 178 119 237 2.13e-16 77.8
MS.gene045148.t1 MTR_5g013010 40.625 128 68 2 51 178 128 247 4.27e-16 77.4
MS.gene045148.t1 MTR_1g054285 37.063 143 81 2 42 184 137 270 6.13e-16 77.0
MS.gene045148.t1 MTR_5g014890 42.718 103 52 1 51 153 115 210 1.69e-15 75.5
MS.gene045148.t1 MTR_8g006705 41.322 121 61 3 59 178 146 257 3.78e-15 74.7
MS.gene045148.t1 MTR_4g084750 41.346 104 53 2 54 156 60 156 4.64e-15 72.8
MS.gene045148.t1 MTR_7g093430 36.478 159 80 4 32 178 121 270 1.24e-14 73.2
MS.gene045148.t1 MTR_4g126900 45.055 91 43 1 62 152 91 174 2.41e-14 71.2
MS.gene045148.t1 MTR_3g103590 50.000 72 36 0 59 130 145 216 3.21e-12 66.2
MS.gene045148.t1 MTR_5g013010 55.000 60 27 0 51 110 128 187 4.04e-11 61.6
MS.gene045148.t1 MTR_2g038625 37.288 118 64 3 42 156 63 173 5.12e-11 61.6
MS.gene045148.t1 MTR_2g038580 37.288 118 64 3 42 156 63 173 5.12e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045148.t1 AT4G40060 46.965 313 135 15 1 301 1 294 8.88e-70 218
MS.gene045148.t1 AT2G22430 44.880 332 130 14 1 301 2 311 3.67e-62 199
MS.gene045148.t1 AT5G65310 41.994 331 142 12 1 301 1 311 3.26e-61 197
MS.gene045148.t1 AT5G65310 43.750 288 118 9 38 301 26 293 9.55e-60 192
MS.gene045148.t1 AT3G01470 62.857 105 38 1 58 161 67 171 2.47e-37 134
MS.gene045148.t1 AT1G69780 54.167 120 52 1 46 165 72 188 1.20e-35 130
MS.gene045148.t1 AT5G15150 47.799 159 79 2 42 197 95 252 5.59e-35 129
MS.gene045148.t1 AT3G01220 53.097 113 51 1 44 154 70 182 9.98e-32 119
MS.gene045148.t1 AT2G46680 40.123 162 91 2 50 205 23 184 6.34e-30 114
MS.gene045148.t1 AT2G46680 40.000 160 92 2 50 205 23 182 6.83e-29 111
MS.gene045148.t1 AT1G26960 43.448 145 75 2 57 201 69 206 1.69e-28 110
MS.gene045148.t1 AT3G61890 47.872 94 49 0 62 155 33 126 3.29e-25 101
MS.gene045148.t1 AT1G27045 48.696 115 59 0 45 159 54 168 4.59e-25 100
MS.gene045148.t1 AT1G27045 48.696 115 59 0 45 159 11 125 7.55e-25 99.0
MS.gene045148.t1 AT1G27045 48.696 115 59 0 45 159 11 125 7.55e-25 99.0
MS.gene045148.t1 AT1G27045 48.696 115 59 0 45 159 3 117 9.19e-25 98.6
MS.gene045148.t1 AT5G03790 43.200 125 55 3 59 180 77 188 1.02e-24 100
MS.gene045148.t1 AT1G27045 48.598 107 55 0 45 151 54 160 3.44e-23 96.3
MS.gene045148.t1 AT5G03790 50.000 90 38 1 59 148 77 159 1.76e-22 92.8
MS.gene045148.t1 AT4G36740 47.706 109 50 1 52 160 49 150 3.34e-22 92.8
MS.gene045148.t1 AT4G36740 47.706 109 50 1 52 160 48 149 3.55e-22 92.8
MS.gene045148.t1 AT2G18550 45.038 131 57 3 46 173 50 168 3.95e-22 92.4
MS.gene045148.t1 AT5G66700 62.162 74 28 0 59 132 71 144 5.44e-22 92.4
MS.gene045148.t1 AT2G36610 40.310 129 60 3 38 158 52 171 1.56e-21 90.1
MS.gene045148.t1 AT5G53980 52.113 71 34 0 59 129 11 81 2.69e-16 75.1
MS.gene045148.t1 AT2G01430 38.129 139 73 5 42 179 122 248 4.08e-16 77.0
MS.gene045148.t1 AT4G16780 39.062 128 70 2 51 178 121 240 5.38e-16 77.0
MS.gene045148.t1 AT4G37790 42.718 103 52 1 51 153 118 213 1.16e-15 75.9
MS.gene045148.t1 AT2G22800 44.660 103 50 1 51 153 105 200 2.47e-15 75.1
MS.gene045148.t1 AT5G06710 35.758 165 81 5 57 221 188 327 6.34e-15 74.7
MS.gene045148.t1 AT4G17460 43.000 100 50 1 54 153 130 222 3.38e-14 72.0
MS.gene045148.t1 AT5G47370 42.718 103 52 1 51 153 122 217 4.65e-14 71.6
MS.gene045148.t1 AT2G44910 39.167 120 65 2 59 178 163 274 1.87e-13 70.1
MS.gene045148.t1 AT3G60390 38.525 122 67 2 57 178 160 273 1.13e-12 67.8
MS.gene045148.t1 AT2G01430 45.714 70 38 0 42 111 122 191 9.22e-11 60.8

Find 59 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATCTTGCTCTCCTGTTT+TGG 0.182991 3.1:+69555772 None:intergenic
TCTTCACATGATAAGATTTC+TGG 0.185572 3.1:+69554763 None:intergenic
GTCATAATCCAATTGATCTT+TGG 0.265734 3.1:+69554962 None:intergenic
CCATACACATGTTTGTTCCT+TGG 0.325978 3.1:+69556008 None:intergenic
AAGCAGTTGGAAAGAGATTA+TGG 0.339996 3.1:-69555744 MS.gene045148:CDS
CAATTCATGGAGGAAGATTC+AGG 0.342389 3.1:+69554795 None:intergenic
GGAATTGATGTTTCTGAAGA+TGG 0.346885 3.1:+69554996 None:intergenic
TTCAGAATCCAAAGATCAAT+TGG 0.360161 3.1:-69554970 MS.gene045148:CDS
TAGCTGTTTGGTTCCAAAAC+AGG 0.367018 3.1:-69555785 MS.gene045148:CDS
GAGAAGCTTGCCATTGAACT+TGG 0.376848 3.1:-69555825 MS.gene045148:CDS
ATTAATGATTAACCTGTTGA+TGG 0.378350 3.1:+69556179 None:intergenic
TCCAGCGCAATTTCTTCATC+CGG 0.382294 3.1:-69554852 MS.gene045148:CDS
TGATCTTTGGATTCTGAACT+AGG 0.386464 3.1:+69554975 None:intergenic
AGTGGATCAAGTTAAGGCTT+TGG 0.405768 3.1:-69555892 MS.gene045148:CDS
AGCCTAGACAAGTAGCTGTT+TGG 0.421533 3.1:-69555797 MS.gene045148:CDS
CTGGTACTGGAAACAATTCA+TGG 0.434538 3.1:+69554782 None:intergenic
ATTAAGAGTGGATCAAGTTA+AGG 0.434553 3.1:-69555898 MS.gene045148:CDS
CCAGCGCAATTTCTTCATCC+GGG 0.438421 3.1:-69554851 MS.gene045148:CDS
GAAGGGTGTGTTGAAGAAAC+TGG 0.440606 3.1:-69555945 MS.gene045148:CDS
ATCACAATCTCTCCATCAAC+AGG 0.446409 3.1:-69556191 MS.gene045148:intron
CATGATAAGATTTCTGGTAC+TGG 0.447688 3.1:+69554769 None:intergenic
AGACTTGAAAGACGGTTCTT+CGG 0.453631 3.1:-69554883 MS.gene045148:CDS
GAACCGTCTTTCAAGTCTAC+TGG 0.456218 3.1:+69554888 None:intergenic
TTGGATGGATTTGAAGAAGA+AGG 0.464593 3.1:-69555963 MS.gene045148:CDS
TTTCAATCAATGATGTTGGA+TGG 0.484310 3.1:-69555978 MS.gene045148:CDS
TGAAGAAGAATCTGAGGTGT+TGG 0.489416 3.1:+69556223 None:intergenic
CCAAGGAACAAACATGTGTA+TGG 0.493903 3.1:-69556008 MS.gene045148:CDS
GGTCAGGACAGTACCATTGT+AGG 0.494776 3.1:+69554673 None:intergenic
AACAACAATGATGTTGGTAT+TGG 0.500792 3.1:-69554930 MS.gene045148:CDS
GAACAAACATGTGTATGGTA+TGG 0.501372 3.1:-69556003 MS.gene045148:CDS
AGGAGTTGAAGAAGAATCTG+AGG 0.515030 3.1:+69556217 None:intergenic
GGAGTTTCAATCAATGATGT+TGG 0.518927 3.1:-69555982 MS.gene045148:CDS
GAAGAAGAATCTGAGGTGTT+GGG 0.524763 3.1:+69556224 None:intergenic
GAATTGATGTTTCTGAAGAT+GGG 0.525493 3.1:+69554997 None:intergenic
GAAATCTTATCATGTGAAGA+TGG 0.526190 3.1:-69554760 MS.gene045148:CDS
AGGCTGCAACCCAAGTTCAA+TGG 0.529892 3.1:+69555815 None:intergenic
CCCGGATGAAGAAATTGCGC+TGG 0.538230 3.1:+69554851 None:intergenic
AAGGAATTGAAATCAAAGCT+TGG 0.543819 3.1:-69555101 MS.gene045148:CDS
AAGATTCAGGAGACAACAAA+AGG 0.555354 3.1:+69554808 None:intergenic
AGAAGCTTGCCATTGAACTT+GGG 0.559661 3.1:-69555824 MS.gene045148:CDS
ATAATCAACAACAATGATGT+TGG 0.562748 3.1:-69554936 MS.gene045148:CDS
CAGCGCAATTTCTTCATCCG+GGG 0.572338 3.1:-69554850 MS.gene045148:CDS
GGACAGTACCATTGTAGGGT+TGG 0.574109 3.1:+69554678 None:intergenic
TCCAAAACAGGAGAGCAAGA+TGG 0.577020 3.1:-69555773 MS.gene045148:CDS
GTCAGGACAGTACCATTGTA+GGG 0.584883 3.1:+69554674 None:intergenic
GAAGAAACGTAGATTAAGAG+TGG 0.588824 3.1:-69555910 MS.gene045148:CDS
AACCAAACAGCTACTTGTCT+AGG 0.590010 3.1:+69555795 None:intergenic
CAATTTCTTAAGTGCCGATG+AGG 0.590118 3.1:-69554730 MS.gene045148:CDS
TGGATGGATTTGAAGAAGAA+GGG 0.596251 3.1:-69555962 MS.gene045148:CDS
TTTCCAGTAGACTTGAAAGA+CGG 0.606889 3.1:-69554891 MS.gene045148:CDS
AAGATGGAAGAACAAGCAGT+TGG 0.616169 3.1:-69555757 MS.gene045148:CDS
ATTTAGAGGAACATAGTCCA+AGG 0.623460 3.1:-69556025 MS.gene045148:intron
TTCATCACAAGATTCAAGCA+TGG 0.624852 3.1:+69555031 None:intergenic
AATGGTACTGTCCTGACCAG+TGG 0.629087 3.1:-69554668 MS.gene045148:CDS
CTGCTGACTCTGAAGAACCC+CGG 0.637223 3.1:+69554833 None:intergenic
AGCAAGCACCAACCCTACAA+TGG 0.659727 3.1:-69554686 MS.gene045148:CDS
ATCTTATCATGTGAAGATGG+AGG 0.661383 3.1:-69554757 MS.gene045148:CDS
GATGGAGAGATTGTGATGAG+AGG 0.700166 3.1:+69556197 None:intergenic
GTACTGGAAACAATTCATGG+AGG 0.765506 3.1:+69554785 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTCTATTTT+TGG - chr3.1:69554768-69554787 MS.gene045148:CDS 10.0%
!! AACGTAAAAGTAAAAAAACA+TGG + chr3.1:69554800-69554819 None:intergenic 20.0%
!!! ATGTTTTTTTACTTTTACGT+TGG - chr3.1:69554799-69554818 MS.gene045148:CDS 20.0%
!!! TTTGTTGTATTTACAGATTA+AGG - chr3.1:69555775-69555794 MS.gene045148:CDS 20.0%
!!! TTTTTCTTTCTATTTTTGGA+GGG - chr3.1:69554772-69554791 MS.gene045148:CDS 20.0%
!!! TTTTTTCTTTCTATTTTTGG+AGG - chr3.1:69554771-69554790 MS.gene045148:CDS 20.0%
! ATAATCAACAACAATGATGT+TGG - chr3.1:69555959-69555978 MS.gene045148:CDS 25.0%
! ATTAATGATTAACCTGTTGA+TGG + chr3.1:69554719-69554738 None:intergenic 25.0%
! GATCTTAGTTATGAAATTGT+AGG - chr3.1:69555654-69555673 MS.gene045148:intron 25.0%
! TATTTAGTGTATGTTTGGAT+TGG - chr3.1:69555564-69555583 MS.gene045148:intron 25.0%
! TGAATTTAGCAAAATCACTT+TGG - chr3.1:69555590-69555609 MS.gene045148:intron 25.0%
! TTATAGTTAAGTTACTACTG+TGG + chr3.1:69555273-69555292 None:intergenic 25.0%
!!! AAAAAGCACAATCAATGTTT+TGG - chr3.1:69555826-69555845 MS.gene045148:CDS 25.0%
!!! CTTTTTTTGCATTGATTTAG+AGG - chr3.1:69554856-69554875 MS.gene045148:CDS 25.0%
!!! TGTGTTATTTAGTGTATGTT+TGG - chr3.1:69555559-69555578 MS.gene045148:intron 25.0%
AACAACAATGATGTTGGTAT+TGG - chr3.1:69555965-69555984 MS.gene045148:CDS 30.0%
AAGGAATTGAAATCAAAGCT+TGG - chr3.1:69555794-69555813 MS.gene045148:CDS 30.0%
ATTAAGAGTGGATCAAGTTA+AGG - chr3.1:69554997-69555016 MS.gene045148:CDS 30.0%
GAAATCTTATCATGTGAAGA+TGG - chr3.1:69556135-69556154 MS.gene045148:intron 30.0%
GTCATAATCCAATTGATCTT+TGG + chr3.1:69555936-69555955 None:intergenic 30.0%
TCTTCACATGATAAGATTTC+TGG + chr3.1:69556135-69556154 None:intergenic 30.0%
TTCAGAATCCAAAGATCAAT+TGG - chr3.1:69555925-69555944 MS.gene045148:CDS 30.0%
TTTCAATCAATGATGTTGGA+TGG - chr3.1:69554917-69554936 MS.gene045148:CDS 30.0%
! GAATTGATGTTTCTGAAGAT+GGG + chr3.1:69555901-69555920 None:intergenic 30.0%
!! GGCAAGTATTTAACTTTTCT+AGG - chr3.1:69555675-69555694 MS.gene045148:CDS 30.0%
AAGATTCAGGAGACAACAAA+AGG + chr3.1:69556090-69556109 None:intergenic 35.0%
AAGCAGTTGGAAAGAGATTA+TGG - chr3.1:69555151-69555170 MS.gene045148:intron 35.0%
AGATCAAGTACTTTCACAAG+AGG + chr3.1:69555639-69555658 None:intergenic 35.0%
ATCTTATCATGTGAAGATGG+AGG - chr3.1:69556138-69556157 MS.gene045148:intron 35.0%
ATTTAGAGGAACATAGTCCA+AGG - chr3.1:69554870-69554889 MS.gene045148:CDS 35.0%
GAACAAACATGTGTATGGTA+TGG - chr3.1:69554892-69554911 MS.gene045148:CDS 35.0%
GAAGAAACGTAGATTAAGAG+TGG - chr3.1:69554985-69555004 MS.gene045148:CDS 35.0%
GGAGTTTCAATCAATGATGT+TGG - chr3.1:69554913-69554932 MS.gene045148:CDS 35.0%
TGATCTTTGGATTCTGAACT+AGG + chr3.1:69555923-69555942 None:intergenic 35.0%
TTCATCACAAGATTCAAGCA+TGG + chr3.1:69555867-69555886 None:intergenic 35.0%
TTTCCAGTAGACTTGAAAGA+CGG - chr3.1:69556004-69556023 MS.gene045148:CDS 35.0%
! AGACAACAAAGCTTTTCACA+AGG - chr3.1:69555228-69555247 MS.gene045148:intron 35.0%
! GGAATTGATGTTTCTGAAGA+TGG + chr3.1:69555902-69555921 None:intergenic 35.0%
! TGGATGGATTTGAAGAAGAA+GGG - chr3.1:69554933-69554952 MS.gene045148:CDS 35.0%
! TTGGATGGATTTGAAGAAGA+AGG - chr3.1:69554932-69554951 MS.gene045148:CDS 35.0%
!! CATGATAAGATTTCTGGTAC+TGG + chr3.1:69556129-69556148 None:intergenic 35.0%
!!! AATCAATGTTTTGGTGAAGG+AGG - chr3.1:69555835-69555854 MS.gene045148:CDS 35.0%
!!! CACAATCAATGTTTTGGTGA+AGG - chr3.1:69555832-69555851 MS.gene045148:CDS 35.0%
AACCAAACAGCTACTTGTCT+AGG + chr3.1:69555103-69555122 None:intergenic 40.0%
AAGATGGAAGAACAAGCAGT+TGG - chr3.1:69555138-69555157 MS.gene045148:intron 40.0%
AGAAGCTTGCCATTGAACTT+GGG - chr3.1:69555071-69555090 MS.gene045148:CDS 40.0%
AGACTTGAAAGACGGTTCTT+CGG - chr3.1:69556012-69556031 MS.gene045148:CDS 40.0%
AGGAGTTGAAGAAGAATCTG+AGG + chr3.1:69554681-69554700 None:intergenic 40.0%
AGTGGATCAAGTTAAGGCTT+TGG - chr3.1:69555003-69555022 MS.gene045148:CDS 40.0%
ATCACAATCTCTCCATCAAC+AGG - chr3.1:69554704-69554723 MS.gene045148:CDS 40.0%
CAATTCATGGAGGAAGATTC+AGG + chr3.1:69556103-69556122 None:intergenic 40.0%
CCAAGGAACAAACATGTGTA+TGG - chr3.1:69554887-69554906 MS.gene045148:CDS 40.0%
CCATACACATGTTTGTTCCT+TGG + chr3.1:69554890-69554909 None:intergenic 40.0%
GTACTGGAAACAATTCATGG+AGG + chr3.1:69556113-69556132 None:intergenic 40.0%
! CAACAAAGCTTTTCACAAGG+AGG - chr3.1:69555231-69555250 MS.gene045148:intron 40.0%
! CTGGTACTGGAAACAATTCA+TGG + chr3.1:69556116-69556135 None:intergenic 40.0%
! GAAGAAGAATCTGAGGTGTT+GGG + chr3.1:69554674-69554693 None:intergenic 40.0%
! GCAAGCTTCTCTTTTCTTTC+AGG + chr3.1:69555061-69555080 None:intergenic 40.0%
! TAGCTGTTTGGTTCCAAAAC+AGG - chr3.1:69555110-69555129 MS.gene045148:intron 40.0%
! TGAAGAAGAATCTGAGGTGT+TGG + chr3.1:69554675-69554694 None:intergenic 40.0%
!! CAATTTCTTAAGTGCCGATG+AGG - chr3.1:69556165-69556184 MS.gene045148:intron 40.0%
!! GGCTTTGGAGAAAAACTTTG+AGG - chr3.1:69555018-69555037 MS.gene045148:CDS 40.0%
AGCCTAGACAAGTAGCTGTT+TGG - chr3.1:69555098-69555117 MS.gene045148:CDS 45.0%
GAAAAAGTTGCACGCCTCAT+CGG + chr3.1:69556182-69556201 None:intergenic 45.0%
GAGAAGCTTGCCATTGAACT+TGG - chr3.1:69555070-69555089 MS.gene045148:CDS 45.0%
GATGGAGAGATTGTGATGAG+AGG + chr3.1:69554701-69554720 None:intergenic 45.0%
GTCAGGACAGTACCATTGTA+GGG + chr3.1:69556224-69556243 None:intergenic 45.0%
TCCAAAACAGGAGAGCAAGA+TGG - chr3.1:69555122-69555141 MS.gene045148:intron 45.0%
TCCAGCGCAATTTCTTCATC+CGG - chr3.1:69556043-69556062 MS.gene045148:intron 45.0%
! GAACCGTCTTTCAAGTCTAC+TGG + chr3.1:69556010-69556029 None:intergenic 45.0%
! GAAGGGTGTGTTGAAGAAAC+TGG - chr3.1:69554950-69554969 MS.gene045148:CDS 45.0%
! TCCATCTTGCTCTCCTGTTT+TGG + chr3.1:69555126-69555145 None:intergenic 45.0%
!! TGAGGCGTGCAACTTTTTCT+CGG - chr3.1:69556183-69556202 MS.gene045148:intron 45.0%
AATGGTACTGTCCTGACCAG+TGG - chr3.1:69556227-69556246 MS.gene045148:CDS 50.0%
AGCAAGCACCAACCCTACAA+TGG - chr3.1:69556209-69556228 MS.gene045148:CDS 50.0%
AGGCTGCAACCCAAGTTCAA+TGG + chr3.1:69555083-69555102 None:intergenic 50.0%
CAGCGCAATTTCTTCATCCG+GGG - chr3.1:69556045-69556064 MS.gene045148:intron 50.0%
CCAGCGCAATTTCTTCATCC+GGG - chr3.1:69556044-69556063 MS.gene045148:intron 50.0%
GGACAGTACCATTGTAGGGT+TGG + chr3.1:69556220-69556239 None:intergenic 50.0%
GGTCAGGACAGTACCATTGT+AGG + chr3.1:69556225-69556244 None:intergenic 50.0%
CCCGGATGAAGAAATTGCGC+TGG + chr3.1:69556047-69556066 None:intergenic 55.0%
!! CTGCTGACTCTGAAGAACCC+CGG + chr3.1:69556065-69556084 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 69554662 69556255 69554662 ID=MS.gene045148
chr3.1 mRNA 69554662 69556255 69554662 ID=MS.gene045148.t1;Parent=MS.gene045148
chr3.1 exon 69556192 69556255 69556192 ID=MS.gene045148.t1.exon1;Parent=MS.gene045148.t1
chr3.1 CDS 69556192 69556255 69556192 ID=cds.MS.gene045148.t1;Parent=MS.gene045148.t1
chr3.1 exon 69555665 69556041 69555665 ID=MS.gene045148.t1.exon2;Parent=MS.gene045148.t1
chr3.1 CDS 69555665 69556041 69555665 ID=cds.MS.gene045148.t1;Parent=MS.gene045148.t1
chr3.1 exon 69554662 69555126 69554662 ID=MS.gene045148.t1.exon3;Parent=MS.gene045148.t1
chr3.1 CDS 69554662 69555126 69554662 ID=cds.MS.gene045148.t1;Parent=MS.gene045148.t1
Gene Sequence

>MS.gene045148

ATGAAGAGACCCAACACCTCAGATTCTTCTTCAACTCCTCTCATCACAATCTCTCCATCAACAGGTTAATCATTAATTTCAGACATAGATCTATGTTTGTGTTTTTTTTTTTTTTCTTTCTATTTTTGGAGGGTACCATGTTTTTTTACTTTTACGTTGGTTATAGTAATTATGTTACTATTACTTATAGTTTTCTTTTTTTGCATTGATTTAGAGGAACATAGTCCAAGGAACAAACATGTGTATGGTATGGAGTTTCAATCAATGATGTTGGATGGATTTGAAGAAGAAGGGTGTGTTGAAGAAACTGGTCATCATTCTGAGAAGAAACGTAGATTAAGAGTGGATCAAGTTAAGGCTTTGGAGAAAAACTTTGAGGTAGAAAACAAGCTTGAACCTGAAAGAAAAGAGAAGCTTGCCATTGAACTTGGGTTGCAGCCTAGACAAGTAGCTGTTTGGTTCCAAAACAGGAGAGCAAGATGGAAGAACAAGCAGTTGGAAAGAGATTATGGTGTTCTTAAAGCTAATTATGATGCTCTTAAGCTCAAGTTTGATGCAATTGCTCAAGACAACAAAGCTTTTCACAAGGAGGTATAAACATCTATAATCCACAGTAGTAACTTAACTATAATTTAGTTTCTGAAGTTATAAGTGCAATAACAACTTAATTAAGTGTTTATAGCATAATCACTTATCATTTAAGTACTTTCGAATAAGCTACTTCATAATATAAGATAGAATAAAGTCACTAGTCAAACTATTTTCATGTAAGTTATTAGTTGTTTTCATAAATTATTCTAAAGCGCTTGTGAAAATAAGCTAAAAACAACTTATTTATAAGTTGTTTCTATAAAAAACAACTTATTTGATATATCAATTCACAGATTTTTAAGCTGTTGTGTTATTTAGTGTATGTTTGGATTGGCAGTGAATTTAGCAAAATCACTTTGGCACTTGAATTTGTAAAGCTATGTCCTCTTGTGAAAGTACTTGATCTTAGTTATGAAATTGTAGGCAAGTATTTAACTTTTCTAGGAACAAATGTATCATAAATTTGAAGTTATTATTGAAAGTGTGTGATTAATTTTCTCTCAAAAACAGAAAGTTGATTGATTTGTTGTATTTACAGATTAAGGAATTGAAATCAAAGCTTGGAGAAGAAGAAAAAAGCACAATCAATGTTTTGGTGAAGGAGGAGTTAACCATGCTTGAATCTTGTGATGAAGACAAGCATAACCCATCTTCAGAAACATCAATTCCTAGTTCAGAATCCAAAGATCAATTGGATTATGACTGCATAATCAACAACAATGATGTTGGTATTGGTGAAACTTCTTCACTCTTTCCAGTAGACTTGAAAGACGGTTCTTCGGACAGCGACTCCAGCGCAATTTCTTCATCCGGGGTTCTTCAGAGTCAGCAGCACCTTTTGTTGTCTCCTGAATCTTCCTCCATGAATTGTTTCCAGTACCAGAAATCTTATCATGTGAAGATGGAGGAGCACAATTTCTTAAGTGCCGATGAGGCGTGCAACTTTTTCTCGGACGAGCAAGCACCAACCCTACAATGGTACTGTCCTGACCAGTGGAGTTAA
Protein sequence

>MS.gene045148.t1

MKRPNTSDSSSTPLITISPSTEEHSPRNKHVYGMEFQSMMLDGFEEEGCVEETGHHSEKKRRLRVDQVKALEKNFEVENKLEPERKEKLAIELGLQPRQVAVWFQNRRARWKNKQLERDYGVLKANYDALKLKFDAIAQDNKAFHKEIKELKSKLGEEEKSTINVLVKEELTMLESCDEDKHNPSSETSIPSSESKDQLDYDCIINNNDVGIGETSSLFPVDLKDGSSDSDSSAISSSGVLQSQQHLLLSPESSSMNCFQYQKSYHVKMEEHNFLSADEACNFFSDEQAPTLQWYCPDQWS