AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045639

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045639.t1 MTR_5g041530 88.860 386 38 1 1 386 1 381 0.0 655
MS.gene045639.t1 MTR_5g041380 63.384 396 110 9 1 386 1 371 2.02e-155 442
MS.gene045639.t1 MTR_5g041400 62.437 394 109 10 1 386 1 363 4.46e-149 426
MS.gene045639.t1 MTR_5g041420 72.787 305 71 4 1 297 1 301 3.71e-142 407
MS.gene045639.t1 MTR_4g082060 44.604 417 130 16 69 384 47 463 1.04e-90 281
MS.gene045639.t1 MTR_7g010950 36.970 495 186 15 2 384 4 484 6.33e-70 228
MS.gene045639.t1 MTR_6g012450 37.292 421 147 13 80 384 74 493 1.13e-64 214
MS.gene045639.t1 MTR_3g435480 36.496 411 153 14 69 384 66 463 2.80e-59 199
MS.gene045639.t1 MTR_8g044220 32.133 361 142 10 115 386 93 439 1.64e-47 167
MS.gene045639.t1 MTR_6g027460 55.140 107 45 1 98 201 51 157 8.62e-33 126
MS.gene045639.t1 MTR_6g027450 53.846 104 45 1 101 201 47 150 1.17e-30 120
MS.gene045639.t1 MTR_7g086780 42.138 159 90 2 78 235 36 193 2.06e-30 121
MS.gene045639.t1 MTR_2g016030 77.966 59 13 0 135 193 42 100 1.91e-29 112
MS.gene045639.t1 MTR_8g015840 66.667 63 17 1 133 191 9 71 3.55e-24 99.8
MS.gene045639.t1 MTR_2g096740 58.571 70 29 0 124 193 46 115 4.62e-23 98.2
MS.gene045639.t1 MTR_2g096740 58.571 70 29 0 124 193 61 130 5.03e-23 98.6
MS.gene045639.t1 MTR_4g022370 56.522 69 30 0 125 193 67 135 1.03e-22 98.6
MS.gene045639.t1 MTR_8g479350 68.966 58 17 1 142 198 70 127 3.47e-22 96.7
MS.gene045639.t1 MTR_3g091820 58.209 67 28 0 127 193 30 96 4.27e-22 95.9
MS.gene045639.t1 MTR_8g079060 53.846 78 26 1 125 192 4 81 5.11e-22 94.0
MS.gene045639.t1 MTR_8g068210 52.703 74 34 1 122 194 14 87 5.89e-22 95.9
MS.gene045639.t1 MTR_7g024670 46.078 102 42 3 105 205 54 143 6.69e-22 96.3
MS.gene045639.t1 MTR_4g109980 69.231 52 16 0 139 190 39 90 1.18e-21 94.7
MS.gene045639.t1 MTR_3g090430 58.904 73 28 2 127 197 36 108 1.46e-21 94.7
MS.gene045639.t1 MTR_8g027295 62.712 59 22 0 135 193 19 77 1.58e-21 93.6
MS.gene045639.t1 MTR_2g059540 68.627 51 16 0 138 188 29 79 1.83e-21 91.7
MS.gene045639.t1 MTR_2g093220 60.606 66 25 1 129 193 28 93 2.67e-21 93.2
MS.gene045639.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 3.68e-21 92.4
MS.gene045639.t1 MTR_4g063780 63.934 61 21 1 136 195 71 131 4.23e-21 93.6
MS.gene045639.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 4.40e-21 92.8
MS.gene045639.t1 MTR_4g088580 71.154 52 15 0 142 193 57 108 1.86e-20 92.4
MS.gene045639.t1 MTR_7g059400 60.656 61 23 1 133 193 70 129 4.28e-20 90.9
MS.gene045639.t1 MTR_4g461080 60.000 65 25 1 129 192 17 81 4.73e-20 89.4
MS.gene045639.t1 MTR_5g031440 63.636 55 20 0 136 190 39 93 6.09e-20 90.1
MS.gene045639.t1 MTR_1g056810 67.308 52 17 0 142 193 14 65 8.55e-20 89.0
MS.gene045639.t1 MTR_4g089095 62.264 53 20 0 142 194 52 104 1.79e-19 88.2
MS.gene045639.t1 MTR_4g089095 62.264 53 20 0 142 194 18 70 1.88e-19 87.4
MS.gene045639.t1 MTR_2g013370 68.519 54 16 1 140 193 46 98 3.12e-19 87.0
MS.gene045639.t1 MTR_2g014060 66.000 50 17 0 142 191 27 76 9.39e-19 86.7
MS.gene045639.t1 MTR_3g077750 62.963 54 20 0 137 190 58 111 1.29e-18 86.3
MS.gene045639.t1 MTR_2g014170 67.347 49 16 0 140 188 45 93 1.37e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045639.t1 AT5G62430 44.828 290 132 10 109 386 25 298 2.73e-64 207
MS.gene045639.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.65e-58 195
MS.gene045639.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.65e-58 195
MS.gene045639.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.65e-58 195
MS.gene045639.t1 AT1G69570 36.499 337 147 16 98 386 80 397 1.08e-46 164
MS.gene045639.t1 AT1G26790 34.875 281 140 11 135 386 106 372 4.80e-38 140
MS.gene045639.t1 AT1G29160 73.913 69 15 1 125 193 50 115 1.49e-32 120
MS.gene045639.t1 AT2G34140 57.143 91 27 2 125 206 46 133 1.02e-30 115
MS.gene045639.t1 AT3G47500 63.415 82 30 0 122 203 92 173 4.71e-30 120
MS.gene045639.t1 AT3G47500 42.945 163 70 6 240 386 293 448 1.02e-23 102
MS.gene045639.t1 AT1G51700 73.077 52 14 0 140 191 33 84 4.89e-23 95.9
MS.gene045639.t1 AT1G07640 54.545 77 29 1 123 199 10 80 5.84e-23 97.8
MS.gene045639.t1 AT5G60200 45.098 102 39 1 92 193 24 108 6.85e-23 97.1
MS.gene045639.t1 AT1G07640 54.545 77 29 1 123 199 66 136 7.68e-23 98.6
MS.gene045639.t1 AT1G07640 54.545 77 29 1 123 199 74 144 1.06e-22 98.2
MS.gene045639.t1 AT2G37590 54.412 68 31 0 126 193 75 142 1.96e-22 97.4
MS.gene045639.t1 AT5G60850 70.370 54 16 0 140 193 51 104 2.62e-22 96.7
MS.gene045639.t1 AT2G28810 48.810 84 40 2 108 191 48 128 4.13e-22 96.3
MS.gene045639.t1 AT3G21270 74.510 51 13 0 142 192 31 81 4.54e-22 93.6
MS.gene045639.t1 AT2G28810 48.810 84 40 2 108 191 65 145 5.58e-22 96.3
MS.gene045639.t1 AT3G61850 38.017 121 72 1 73 193 10 127 7.39e-22 95.1
MS.gene045639.t1 AT3G61850 38.017 121 72 1 73 193 10 127 1.16e-21 95.1
MS.gene045639.t1 AT3G55370 60.317 63 24 1 131 193 68 129 1.43e-21 94.7
MS.gene045639.t1 AT3G55370 60.317 63 24 1 131 193 68 129 1.79e-21 95.1
MS.gene045639.t1 AT3G55370 60.317 63 24 1 131 193 113 174 1.96e-21 95.1
MS.gene045639.t1 AT4G24060 60.000 65 26 0 129 193 42 106 2.49e-21 94.4
MS.gene045639.t1 AT3G50410 69.231 52 16 0 140 191 30 81 3.37e-21 92.4
MS.gene045639.t1 AT4G38000 51.948 77 36 1 139 214 40 116 4.06e-21 92.0
MS.gene045639.t1 AT3G61850 57.576 66 28 0 128 193 50 115 6.22e-21 92.0
MS.gene045639.t1 AT3G61850 57.576 66 28 0 128 193 50 115 6.22e-21 92.0
MS.gene045639.t1 AT3G45610 43.564 101 55 1 133 231 33 133 8.98e-21 90.9
MS.gene045639.t1 AT3G61850 57.576 66 28 0 128 193 50 115 9.52e-21 92.0
MS.gene045639.t1 AT5G65590 53.750 80 29 2 119 193 18 94 1.15e-20 92.0
MS.gene045639.t1 AT3G52440 48.315 89 45 1 137 224 22 110 2.08e-20 90.1
MS.gene045639.t1 AT5G02460 62.069 58 22 0 136 193 91 148 2.93e-20 92.0
MS.gene045639.t1 AT3G52440 48.315 89 45 1 137 224 41 129 3.39e-20 89.7
MS.gene045639.t1 AT2G28510 67.308 52 17 0 142 193 50 101 5.66e-20 89.7
MS.gene045639.t1 AT1G28310 66.667 54 18 0 140 193 27 80 5.79e-20 90.1
MS.gene045639.t1 AT1G28310 66.667 54 18 0 140 193 51 104 8.33e-20 89.7
MS.gene045639.t1 AT5G66940 66.667 51 17 0 142 192 34 84 9.74e-20 87.4
MS.gene045639.t1 AT1G21340 59.677 62 24 1 138 198 35 96 1.88e-19 87.4
MS.gene045639.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.92e-19 88.2
MS.gene045639.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.92e-19 88.2
MS.gene045639.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.92e-19 88.2
MS.gene045639.t1 AT1G64620 63.462 52 19 0 137 188 46 97 2.01e-19 89.0
MS.gene045639.t1 AT1G47655 65.306 49 17 0 140 188 29 77 4.57e-19 85.1
MS.gene045639.t1 AT4G21050 67.308 52 17 0 142 193 26 77 9.51e-19 84.3
MS.gene045639.t1 AT2G46590 60.377 53 21 0 135 187 63 115 6.00e-18 84.7
MS.gene045639.t1 AT2G46590 60.377 53 21 0 135 187 75 127 6.41e-18 84.7
MS.gene045639.t1 AT5G62940 67.347 49 16 0 142 190 75 123 6.70e-18 84.7
MS.gene045639.t1 AT4G21040 61.538 52 20 0 142 193 27 78 8.79e-17 79.3
MS.gene045639.t1 AT4G21080 61.538 52 20 0 142 193 27 78 3.58e-16 77.8

Find 72 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTCTTTGCAATTTCATT+AGG 0.246474 5.3:-29093288 None:intergenic
GGTCAAAATTCAACTACATT+AGG 0.249796 5.3:+29093150 MS.gene045639:CDS
ACCGCCATAGTATGCTATAT+TGG 0.313531 5.3:-29093079 None:intergenic
TCATCGATTCTTAGTGTCTT+AGG 0.320046 5.3:-29093264 None:intergenic
ACAATTATGTCCGGTTTCAT+TGG 0.357374 5.3:-29092703 None:intergenic
AACTCTTTGCAATTTCATTA+GGG 0.357550 5.3:-29093287 None:intergenic
GTCAAAATTCAACTACATTA+GGG 0.362039 5.3:+29093151 MS.gene045639:CDS
TGCCAGAGGTATTGGACTTC+CGG 0.368367 5.3:+29092814 MS.gene045639:CDS
TCTTCCTCAGAGTTAGTGTT+TGG 0.376912 5.3:+29091585 MS.gene045639:CDS
TTAGGGAAACATTCAAGAAA+TGG 0.383950 5.3:+29093168 MS.gene045639:CDS
TCGGTTGGAGAATTCACTTC+AGG 0.387221 5.3:-29091669 None:intergenic
CCGCCATAGTATGCTATATT+GGG 0.397382 5.3:-29093078 None:intergenic
TATCCCAATATAGCATACTA+TGG 0.401203 5.3:+29093075 MS.gene045639:CDS
AGTACTACTATCTTGACCTT+TGG 0.413423 5.3:+29092910 MS.gene045639:CDS
AGCATACTATGGCGGTTGTT+TGG 0.417812 5.3:+29093086 MS.gene045639:CDS
GGGGAACATGAAGATGAATA+AGG 0.417962 5.3:-29091645 None:intergenic
CAGTTTAGAAGTTTCTGTTT+CGG 0.422590 5.3:-29092632 None:intergenic
AAATGATGGAAAGAGATATA+AGG 0.429316 5.3:+29091692 MS.gene045639:CDS
CATATTTCTCGTTGCTCCTC+CGG 0.432490 5.3:-29092833 None:intergenic
GACACAGGATAACACATTGC+AGG 0.443245 5.3:-29093048 None:intergenic
CAACACTAGGAATCAAGAAC+GGG 0.446060 5.3:+29093319 MS.gene045639:CDS
GAGGAATCTTCATGAGGAAA+AGG 0.451641 5.3:-29091612 None:intergenic
GGGTTCTCAACAAGGCTTGA+AGG 0.457419 5.3:-29092595 None:intergenic
TAACTCTGAGGAAGAATCAT+TGG 0.468143 5.3:-29091577 None:intergenic
TAGGGAAACATTCAAGAAAT+GGG 0.472410 5.3:+29093169 MS.gene045639:CDS
GCAACGAGAAATATGATTGT+AGG 0.472417 5.3:+29092841 MS.gene045639:CDS
TTCATGGGATTCGCATCCAA+AGG 0.488816 5.3:+29093351 MS.gene045639:CDS
AGAGAAATCTCCAATGAAAC+CGG 0.492605 5.3:+29092693 MS.gene045639:CDS
GGACAGGGAACAATTATGTC+CGG 0.498365 5.3:-29092712 None:intergenic
CTCCGGAAGTCCAATACCTC+TGG 0.499281 5.3:-29092816 None:intergenic
TCATGGGATTCGCATCCAAA+GGG 0.501709 5.3:+29093352 MS.gene045639:CDS
CTTGACAAGAAAGTGAATGT+TGG 0.504154 5.3:+29092964 MS.gene045639:CDS
GCGGTTGTTTGGTACCATCA+TGG 0.511292 5.3:+29093097 MS.gene045639:CDS
TTCGCAATCAAATCCTCTCA+TGG 0.511946 5.3:-29093452 None:intergenic
AATTCTCCAACCGAAAATGA+TGG 0.512006 5.3:+29091678 MS.gene045639:CDS
AAATTGCAAAGAGTTCTATA+TGG 0.517606 5.3:+29093295 MS.gene045639:CDS
AGAAGTTTCTGTTTCGGACG+AGG 0.520692 5.3:-29092626 None:intergenic
CTAATCCTGTAGCTTTATCA+AGG 0.525653 5.3:+29093418 MS.gene045639:CDS
AGAAATATGATTGTAGGTGC+AGG 0.532367 5.3:+29092847 MS.gene045639:CDS
TTGTGTTGCAGGAAACATCA+TGG 0.539505 5.3:+29092503 MS.gene045639:intron
GAAGTTTCTGTTTCGGACGA+GGG 0.541471 5.3:-29092625 None:intergenic
AGAGACCTTGATAAAGCTAC+AGG 0.544478 5.3:-29093423 None:intergenic
AACAAACTCAATGCAGCCAA+TGG 0.545923 5.3:+29092880 MS.gene045639:CDS
GGTCTCTTGTGTTCCATGAG+AGG 0.551572 5.3:+29093439 MS.gene045639:CDS
CATATCAGTTCAAGAGGATG+AGG 0.559879 5.3:+29092534 MS.gene045639:CDS
AGTAGTACTATGCAATCCAT+TGG 0.564693 5.3:-29092896 None:intergenic
GTTGGAGAATTCACTTCAGG+TGG 0.567909 5.3:-29091666 None:intergenic
AGAACTCATATCAGTTCAAG+AGG 0.588411 5.3:+29092528 MS.gene045639:CDS
AGTAGAAGAATCTGATCCAA+AGG 0.598047 5.3:-29092926 None:intergenic
TTGGAGAATTCACTTCAGGT+GGG 0.604331 5.3:-29091665 None:intergenic
CTGTCCAAGATGCAAAAGCA+TGG 0.610924 5.3:+29092729 MS.gene045639:CDS
ATAGATTGTACATTCCATGA+TGG 0.621316 5.3:-29093111 None:intergenic
AGTTCTATATGGTCAACACT+AGG 0.622239 5.3:+29093306 MS.gene045639:CDS
ACACAGGATAACACATTGCA+GGG 0.622856 5.3:-29093047 None:intergenic
TCAACACTAGGAATCAAGAA+CGG 0.637810 5.3:+29093318 MS.gene045639:CDS
ATGGGATTCGCATCCAAAGG+GGG 0.645601 5.3:+29093354 MS.gene045639:CDS
TGAATAAGGACTATGATCAG+AGG 0.646385 5.3:-29091631 None:intergenic
ATCCTCTGTAGATTGGAATG+AGG 0.648122 5.3:-29092557 None:intergenic
TGGAGAATTCACTTCAGGTG+GGG 0.648645 5.3:-29091664 None:intergenic
TCTATCTCGACTCAATCTTG+TGG 0.657132 5.3:+29093129 MS.gene045639:CDS
ATATTGGGATAGAATGACAC+AGG 0.661691 5.3:-29093063 None:intergenic
CCCAATATAGCATACTATGG+CGG 0.676610 5.3:+29093078 MS.gene045639:CDS
GGGTTCCAAGAAAACTGTGG+AGG 0.676664 5.3:-29093027 None:intergenic
ATTAGCTTGCAAAACTGACG+AGG 0.678686 5.3:-29093400 None:intergenic
TGATCAGAGGAATCTTCATG+AGG 0.678996 5.3:-29091618 None:intergenic
CAGAGGTATTGGACTTCCGG+AGG 0.686773 5.3:+29092817 MS.gene045639:CDS
CATGGGATTCGCATCCAAAG+GGG 0.694560 5.3:+29093353 MS.gene045639:CDS
AGAACCAAACACTAACTCTG+AGG 0.705763 5.3:-29091589 None:intergenic
AACACTAGGAATCAAGAACG+GGG 0.710662 5.3:+29093320 MS.gene045639:CDS
GGCCTCATTCCAATCTACAG+AGG 0.711504 5.3:+29092555 MS.gene045639:CDS
GCAGGGTTCCAAGAAAACTG+TGG 0.717555 5.3:-29093030 None:intergenic
ACACTAGGAATCAAGAACGG+GGG 0.749858 5.3:+29093321 MS.gene045639:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTAAAATATCAAGA+AGG + chr5.3:29092239-29092258 MS.gene045639:intron 10.0%
!! AGAAATTAGAAATTGTTAAT+GGG + chr5.3:29091825-29091844 MS.gene045639:intron 15.0%
!! AAAAACTTTATCTTAATCAG+TGG + chr5.3:29092147-29092166 MS.gene045639:intron 20.0%
!! ATATACAACAGAATATCATA+TGG - chr5.3:29092266-29092285 None:intergenic 20.0%
!! CAGAAATTAGAAATTGTTAA+TGG + chr5.3:29091824-29091843 MS.gene045639:intron 20.0%
!!! ACTTTGTTCATCAATTTTAT+GGG - chr5.3:29092662-29092681 None:intergenic 20.0%
! AAATTGCAAAGAGTTCTATA+TGG + chr5.3:29093295-29093314 MS.gene045639:CDS 25.0%
! AACTCTTTGCAATTTCATTA+GGG - chr5.3:29093290-29093309 None:intergenic 25.0%
! ATTGACATTATAAAGCTATG+AGG - chr5.3:29092467-29092486 None:intergenic 25.0%
! GAATGTAAGTATATGTATCT+GGG - chr5.3:29092374-29092393 None:intergenic 25.0%
! GTCAAAATTCAACTACATTA+GGG + chr5.3:29093151-29093170 MS.gene045639:CDS 25.0%
! GTTATTCATTGTGATTAAAC+CGG - chr5.3:29092073-29092092 None:intergenic 25.0%
! TCATTGTTAATGATCTTACA+TGG + chr5.3:29092118-29092137 MS.gene045639:intron 25.0%
! TGAATGTAAGTATATGTATC+TGG - chr5.3:29092375-29092394 None:intergenic 25.0%
!! AAATGATGGAAAGAGATATA+AGG + chr5.3:29091692-29091711 MS.gene045639:CDS 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5.3:29091516-29091535 MS.gene045639:CDS 25.0%
!! ATATCTCTTTCCATCATTTT+CGG - chr5.3:29091691-29091710 None:intergenic 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.3:29091517-29091536 MS.gene045639:CDS 25.0%
!! CACTTTGTTCATCAATTTTA+TGG - chr5.3:29092663-29092682 None:intergenic 25.0%
!!! TTAGTTTTCATGATGATAGA+TGG + chr5.3:29091958-29091977 MS.gene045639:intron 25.0%
AATCAGTATAATTGTGTTGC+AGG + chr5.3:29092492-29092511 MS.gene045639:intron 30.0%
AGTTCTCAAATTAATATCGG+TGG + chr5.3:29092406-29092425 MS.gene045639:intron 30.0%
ATAGATTGTACATTCCATGA+TGG - chr5.3:29093114-29093133 None:intergenic 30.0%
CAGTTTAGAAGTTTCTGTTT+CGG - chr5.3:29092635-29092654 None:intergenic 30.0%
CATATGTATATCCGTAAAAC+CGG + chr5.3:29092051-29092070 MS.gene045639:intron 30.0%
CATGAAAACTAACCAAAAAG+TGG - chr5.3:29091950-29091969 None:intergenic 30.0%
CGCAGTTCTCAAATTAATAT+CGG + chr5.3:29092403-29092422 MS.gene045639:intron 30.0%
GAACTCTTTGCAATTTCATT+AGG - chr5.3:29093291-29093310 None:intergenic 30.0%
GGTCAAAATTCAACTACATT+AGG + chr5.3:29093150-29093169 MS.gene045639:CDS 30.0%
GTTCTCAAATTAATATCGGT+GGG + chr5.3:29092407-29092426 MS.gene045639:intron 30.0%
TAGGGAAACATTCAAGAAAT+GGG + chr5.3:29093169-29093188 MS.gene045639:CDS 30.0%
TATCCCAATATAGCATACTA+TGG + chr5.3:29093075-29093094 MS.gene045639:CDS 30.0%
TTAGGGAAACATTCAAGAAA+TGG + chr5.3:29093168-29093187 MS.gene045639:CDS 30.0%
! ACGCATTTAATCTAAAGTGT+TGG - chr5.3:29092333-29092352 None:intergenic 30.0%
! ATTTTGAATCCTCTGTAGAT+TGG - chr5.3:29092567-29092586 None:intergenic 30.0%
! TGAATGAACTTTTATCCAGA+AGG - chr5.3:29092181-29092200 None:intergenic 30.0%
!! TCATGATTTGTTGACGATTT+CGG - chr5.3:29092026-29092045 None:intergenic 30.0%
!! TTGTGATTAAACCGGTTTTA+CGG - chr5.3:29092065-29092084 None:intergenic 30.0%
AATTCTCCAACCGAAAATGA+TGG + chr5.3:29091678-29091697 MS.gene045639:CDS 35.0%
ACAATTATGTCCGGTTTCAT+TGG - chr5.3:29092706-29092725 None:intergenic 35.0%
AGAAATATGATTGTAGGTGC+AGG + chr5.3:29092847-29092866 MS.gene045639:CDS 35.0%
AGAACTCATATCAGTTCAAG+AGG + chr5.3:29092528-29092547 MS.gene045639:CDS 35.0%
AGAGAAATCTCCAATGAAAC+CGG + chr5.3:29092693-29092712 MS.gene045639:CDS 35.0%
AGCTCAGAGAAAGAAAAACT+TGG + chr5.3:29093210-29093229 MS.gene045639:CDS 35.0%
AGTACTACTATCTTGACCTT+TGG + chr5.3:29092910-29092929 MS.gene045639:CDS 35.0%
AGTAGAAGAATCTGATCCAA+AGG - chr5.3:29092929-29092948 None:intergenic 35.0%
AGTAGTACTATGCAATCCAT+TGG - chr5.3:29092899-29092918 None:intergenic 35.0%
AGTTCTATATGGTCAACACT+AGG + chr5.3:29093306-29093325 MS.gene045639:CDS 35.0%
ATATTGGGATAGAATGACAC+AGG - chr5.3:29093066-29093085 None:intergenic 35.0%
CTTGACAAGAAAGTGAATGT+TGG + chr5.3:29092964-29092983 MS.gene045639:CDS 35.0%
GCAACGAGAAATATGATTGT+AGG + chr5.3:29092841-29092860 MS.gene045639:CDS 35.0%
TCAACACTAGGAATCAAGAA+CGG + chr5.3:29093318-29093337 MS.gene045639:CDS 35.0%
TGAATAAGGACTATGATCAG+AGG - chr5.3:29091634-29091653 None:intergenic 35.0%
TGTTCTCCCAAAAAGCTTTA+TGG - chr5.3:29091526-29091545 None:intergenic 35.0%
! AAGTTTTTCTTTCTCTGAGC+TGG - chr5.3:29093211-29093230 None:intergenic 35.0%
! TAACTCTGAGGAAGAATCAT+TGG - chr5.3:29091580-29091599 None:intergenic 35.0%
!! ATGAGGTATTGACATAGACA+AGG - chr5.3:29092450-29092469 None:intergenic 35.0%
!! CTAATCCTGTAGCTTTATCA+AGG + chr5.3:29093418-29093437 MS.gene045639:CDS 35.0%
!! TCATCGATTCTTAGTGTCTT+AGG - chr5.3:29093267-29093286 None:intergenic 35.0%
!! TGAGGTATTGACATAGACAA+GGG - chr5.3:29092449-29092468 None:intergenic 35.0%
!!! TTGCTGTTTAGACCACTTTT+TGG + chr5.3:29091935-29091954 MS.gene045639:intron 35.0%
AACAAACTCAATGCAGCCAA+TGG + chr5.3:29092880-29092899 MS.gene045639:CDS 40.0%
AACACTAGGAATCAAGAACG+GGG + chr5.3:29093320-29093339 MS.gene045639:CDS 40.0%
ACACAGGATAACACATTGCA+GGG - chr5.3:29093050-29093069 None:intergenic 40.0%
ACCGCCATAGTATGCTATAT+TGG - chr5.3:29093082-29093101 None:intergenic 40.0%
AGAACCAAACACTAACTCTG+AGG - chr5.3:29091592-29091611 None:intergenic 40.0%
AGAGACCTTGATAAAGCTAC+AGG - chr5.3:29093426-29093445 None:intergenic 40.0%
ATCCTCTGTAGATTGGAATG+AGG - chr5.3:29092560-29092579 None:intergenic 40.0%
CAACACTAGGAATCAAGAAC+GGG + chr5.3:29093319-29093338 MS.gene045639:CDS 40.0%
CATATCAGTTCAAGAGGATG+AGG + chr5.3:29092534-29092553 MS.gene045639:CDS 40.0%
CCCAATATAGCATACTATGG+CGG + chr5.3:29093078-29093097 MS.gene045639:CDS 40.0%
CCGCCATAGTATGCTATATT+GGG - chr5.3:29093081-29093100 None:intergenic 40.0%
GAGGAATCTTCATGAGGAAA+AGG - chr5.3:29091615-29091634 None:intergenic 40.0%
GCCAATAAATAGAACCACCA+TGG - chr5.3:29091737-29091756 None:intergenic 40.0%
GGGGAACATGAAGATGAATA+AGG - chr5.3:29091648-29091667 None:intergenic 40.0%
TAAATAGAACCACCATGGCA+TGG - chr5.3:29091732-29091751 None:intergenic 40.0%
TATATGTATCTGGGTCGTGT+CGG - chr5.3:29092365-29092384 None:intergenic 40.0%
TCTATCTCGACTCAATCTTG+TGG + chr5.3:29093129-29093148 MS.gene045639:CDS 40.0%
TCTTCCTCAGAGTTAGTGTT+TGG + chr5.3:29091585-29091604 MS.gene045639:CDS 40.0%
TGATCAGAGGAATCTTCATG+AGG - chr5.3:29091621-29091640 None:intergenic 40.0%
TTGGAGAATTCACTTCAGGT+GGG - chr5.3:29091668-29091687 None:intergenic 40.0%
TTGTGTTGCAGGAAACATCA+TGG + chr5.3:29092503-29092522 MS.gene045639:intron 40.0%
! ATGCTTTTGCATCTTGGACA+GGG - chr5.3:29092730-29092749 None:intergenic 40.0%
! CTCTTTCCATCATTTTCGGT+TGG - chr5.3:29091687-29091706 None:intergenic 40.0%
! CTTTTATCCAGAAGGACTTC+AGG - chr5.3:29092173-29092192 None:intergenic 40.0%
! GCCATGGTGGTTCTATTTAT+TGG + chr5.3:29091733-29091752 MS.gene045639:intron 40.0%
! GTTTACCTCCACAGTTTTCT+TGG + chr5.3:29093022-29093041 MS.gene045639:CDS 40.0%
!! ATTAGCTTGCAAAACTGACG+AGG - chr5.3:29093403-29093422 None:intergenic 40.0%
!! GTGTTGGTGTTACATAGACA+TGG - chr5.3:29092317-29092336 None:intergenic 40.0%
ACACTAGGAATCAAGAACGG+GGG + chr5.3:29093321-29093340 MS.gene045639:CDS 45.0%
ACTTCTGCAAAAACTGCCAG+AGG + chr5.3:29092800-29092819 MS.gene045639:CDS 45.0%
AGAAGTTTCTGTTTCGGACG+AGG - chr5.3:29092629-29092648 None:intergenic 45.0%
AGCATACTATGGCGGTTGTT+TGG + chr5.3:29093086-29093105 MS.gene045639:CDS 45.0%
CATATTTCTCGTTGCTCCTC+CGG - chr5.3:29092836-29092855 None:intergenic 45.0%
CTGTCCAAGATGCAAAAGCA+TGG + chr5.3:29092729-29092748 MS.gene045639:CDS 45.0%
GAAGTTTCTGTTTCGGACGA+GGG - chr5.3:29092628-29092647 None:intergenic 45.0%
GACACAGGATAACACATTGC+AGG - chr5.3:29093051-29093070 None:intergenic 45.0%
GGACAGGGAACAATTATGTC+CGG - chr5.3:29092715-29092734 None:intergenic 45.0%
GTTGGAGAATTCACTTCAGG+TGG - chr5.3:29091669-29091688 None:intergenic 45.0%
TCATGGGATTCGCATCCAAA+GGG + chr5.3:29093352-29093371 MS.gene045639:CDS 45.0%
TCGGTTGGAGAATTCACTTC+AGG - chr5.3:29091672-29091691 None:intergenic 45.0%
TGGAGAATTCACTTCAGGTG+GGG - chr5.3:29091667-29091686 None:intergenic 45.0%
TTCATGGGATTCGCATCCAA+AGG + chr5.3:29093351-29093370 MS.gene045639:CDS 45.0%
! CATGCTTTTGCATCTTGGAC+AGG - chr5.3:29092731-29092750 None:intergenic 45.0%
! GCAAAAACTGCCAGAGGTAT+TGG + chr5.3:29092806-29092825 MS.gene045639:CDS 45.0%
! GTGTCCATGCTTTTGCATCT+TGG - chr5.3:29092736-29092755 None:intergenic 45.0%
!! GGCAGTTTTTGCAGAAGTGT+CGG - chr5.3:29092798-29092817 None:intergenic 45.0%
!!! CAGTTTTTGCAGAAGTGTCG+GGG - chr5.3:29092796-29092815 None:intergenic 45.0%
!!! GAGTTTTCGGGTTCTCAACA+AGG - chr5.3:29092606-29092625 None:intergenic 45.0%
!!! GCAGTTTTTGCAGAAGTGTC+GGG - chr5.3:29092797-29092816 None:intergenic 45.0%
!! ATTTATTTCAAAATAAAATT+TGG - chr5.3:29091856-29091875 None:intergenic 5.0%
ACGATTCTTGACTCCCCCTT+TGG - chr5.3:29093370-29093389 None:intergenic 50.0%
ATGGGATTCGCATCCAAAGG+GGG + chr5.3:29093354-29093373 MS.gene045639:CDS 50.0%
CATGGGATTCGCATCCAAAG+GGG + chr5.3:29093353-29093372 MS.gene045639:CDS 50.0%
GCAGGGTTCCAAGAAAACTG+TGG - chr5.3:29093033-29093052 None:intergenic 50.0%
GCGGTTGTTTGGTACCATCA+TGG + chr5.3:29093097-29093116 MS.gene045639:CDS 50.0%
GGCCTCATTCCAATCTACAG+AGG + chr5.3:29092555-29092574 MS.gene045639:CDS 50.0%
GGGTTCCAAGAAAACTGTGG+AGG - chr5.3:29093030-29093049 None:intergenic 50.0%
GGGTTCTCAACAAGGCTTGA+AGG - chr5.3:29092598-29092617 None:intergenic 50.0%
GGTCTCTTGTGTTCCATGAG+AGG + chr5.3:29093439-29093458 MS.gene045639:CDS 50.0%
TGAGTTCTTGTGCCATGCCA+TGG + chr5.3:29091717-29091736 MS.gene045639:intron 50.0%
! AACGGGGGAGAGCTTTTCAT+GGG + chr5.3:29093336-29093355 MS.gene045639:CDS 50.0%
! GTTTCGGACGAGGGAGTTTT+CGG - chr5.3:29092619-29092638 None:intergenic 50.0%
! TTTCGGACGAGGGAGTTTTC+GGG - chr5.3:29092618-29092637 None:intergenic 50.0%
!! TGCCAGAGGTATTGGACTTC+CGG + chr5.3:29092814-29092833 MS.gene045639:CDS 50.0%
CTCCGGAAGTCCAATACCTC+TGG - chr5.3:29092819-29092838 None:intergenic 55.0%
GTTCTTGTGCCATGCCATGG+TGG + chr5.3:29091720-29091739 MS.gene045639:intron 55.0%
TCAGTGGCCTGAAGTCCTTC+TGG + chr5.3:29092163-29092182 MS.gene045639:intron 55.0%
! GAACGGGGGAGAGCTTTTCA+TGG + chr5.3:29093335-29093354 MS.gene045639:CDS 55.0%
!! CAGAGGTATTGGACTTCCGG+AGG + chr5.3:29092817-29092836 MS.gene045639:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 29091507 29093467 29091507 ID=MS.gene045639
chr5.3 mRNA 29091507 29093467 29091507 ID=MS.gene045639.t1;Parent=MS.gene045639
chr5.3 exon 29091507 29091713 29091507 ID=MS.gene045639.t1.exon1;Parent=MS.gene045639.t1
chr5.3 CDS 29091507 29091713 29091507 ID=cds.MS.gene045639.t1;Parent=MS.gene045639.t1
chr5.3 exon 29092514 29093467 29092514 ID=MS.gene045639.t1.exon2;Parent=MS.gene045639.t1
chr5.3 CDS 29092514 29093467 29092514 ID=cds.MS.gene045639.t1;Parent=MS.gene045639.t1
Gene Sequence

>MS.gene045639

ATGACTAATAATTCAACCATAAAGCTTTTTGGGAGAACAATTTTTCAAACATACAACACAGATGTCTCTACCAATGATTCTTCCTCAGAGTTAGTGTTTGGTTCTCCTTTTCCTCATGAAGATTCCTCTGATCATAGTCCTTATTCATCTTCATGTTCCCCACCTGAAGTGAATTCTCCAACCGAAAATGATGGAAAGAGATATAAGGTATGAGTTCTTGTGCCATGCCATGGTGGTTCTATTTATTGGCTAAAATTTATTATTTAAAATATGTTGCTAACTAGTGTGTTTTGAAATTATAACAGTGCAACTTTTTTCAGAAATTAGAAATTGTTAATGGGTTGATCCAAATTTTATTTTGAAATAAATTGCTTCTAGTTAAGACTTAAGAGTATATAATTATAATTAAGTCTCACAGCAATTTTTCGTTGCTGTTTAGACCACTTTTTGGTTAGTTTTCATGATGATAGATGGTTGATGAGATTGTTATCTTTTCTCATTAGTAGATTTATTTAACCGAAATCGTCAACAAATCATGATCAATCATATGTATATCCGTAAAACCGGTTTAATCACAATGAATAACACGACTCAATCATGATTAAAGTCAATCATTGTTAATGATCTTACATGGTTTTATAAAAACTTTATCTTAATCAGTGGCCTGAAGTCCTTCTGGATAAAAGTTCATTCAAGAATTTCGTGTTCTAAAACATGAGATTGCAAAACTGAAATAAATTAAAATATCAAGAAGGACCATATGATATTCTGTTGTATATTTATGTCAAAACGAATAAATCATTTAGTCCATGTCTATGTAACACCAACACTTTAGATTAAATGCGTGTTTGATGTCCGACACGACCCAGATACATATACTTACATTCAATATTTCACGCAGTTCTCAAATTAATATCGGTGGGAGTGTCGTATTCGATTCCCTTGTCTATGTCAATACCTCATAGCTTTATAATGTCAATTTATAAATCAGTATAATTGTGTTGCAGGAAACATCATGGAAAGAACTCATATCAGTTCAAGAGGATGAGGCCTCATTCCAATCTACAGAGGATTCAAAATCTCACACACCTTCAAGCCTTGTTGAGAACCCGAAAACTCCCTCGTCCGAAACAGAAACTTCTAAACTGAATTCCCATAAAATTGATGAACAAAGTGATATGTCACAAGAGAAATCTCCAATGAAACCGGACATAATTGTTCCCTGTCCAAGATGCAAAAGCATGGACACAAAATTTTGTTACTATAACAACTACAATGTCAAACAGCCCCGACACTTCTGCAAAAACTGCCAGAGGTATTGGACTTCCGGAGGAGCAACGAGAAATATGATTGTAGGTGCAGGTCGTCGCAAGAACAAACTCAATGCAGCCAATGGATTGCATAGTACTACTATCTTGACCTTTGGATCAGATTCTTCTACTATGTCTTCAACTTCACTTGACAAGAAAGTGAATGTTGGATCACATGAAGAAACATTTGATAAGAGTTATCAAAGTTTACCTCCACAGTTTTCTTGGAACCCTGCAATGTGTTATCCTGTGTCATTCTATCCCAATATAGCATACTATGGCGGTTGTTTGGTACCATCATGGAATGTACAATCTATCTCGACTCAATCTTGTGGTCAAAATTCAACTACATTAGGGAAACATTCAAGAAATGGGAACATTCTCGTTTGTTCCAGCTCAGAGAAAGAAAAACTTGGATCAGAAAGTAATAATAATAGTGTGTTCATACCTAAGACACTAAGAATCGATGACCCTAATGAAATTGCAAAGAGTTCTATATGGTCAACACTAGGAATCAAGAACGGGGGAGAGCTTTTCATGGGATTCGCATCCAAAGGGGGAGTCAAGAATCGTGTTGAAACATCCTCGTCAGTTTTGCAAGCTAATCCTGTAGCTTTATCAAGGTCTCTTGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene045639.t1

MTNNSTIKLFGRTIFQTYNTDVSTNDSSSELVFGSPFPHEDSSDHSPYSSSCSPPEVNSPTENDGKRYKETSWKELISVQEDEASFQSTEDSKSHTPSSLVENPKTPSSETETSKLNSHKIDEQSDMSQEKSPMKPDIIVPCPRCKSMDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGATRNMIVGAGRRKNKLNAANGLHSTTILTFGSDSSTMSSTSLDKKVNVGSHEETFDKSYQSLPPQFSWNPAMCYPVSFYPNIAYYGGCLVPSWNVQSISTQSCGQNSTTLGKHSRNGNILVCSSSEKEKLGSESNNNSVFIPKTLRIDDPNEIAKSSIWSTLGIKNGGELFMGFASKGGVKNRVETSSSVLQANPVALSRSLVFHERI