AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045643

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045643.t1 MTR_5g041380 84.856 383 42 2 1 379 1 371 0.0 637
MS.gene045643.t1 MTR_5g041400 83.377 379 47 2 1 379 1 363 0.0 622
MS.gene045643.t1 MTR_5g041530 64.232 397 108 11 1 379 1 381 1.22e-148 425
MS.gene045643.t1 MTR_5g041420 60.925 389 75 9 1 379 1 322 5.47e-128 370
MS.gene045643.t1 MTR_4g082060 38.571 490 155 20 4 378 6 464 2.03e-74 238
MS.gene045643.t1 MTR_7g010950 33.803 497 192 17 2 377 4 484 3.43e-64 212
MS.gene045643.t1 MTR_6g012450 33.465 508 184 20 4 377 6 493 1.06e-52 182
MS.gene045643.t1 MTR_3g435480 32.640 481 198 17 2 377 4 463 3.63e-52 180
MS.gene045643.t1 MTR_8g044220 31.728 353 150 13 111 379 94 439 4.57e-40 147
MS.gene045643.t1 MTR_6g027460 58.586 99 35 2 96 188 51 149 3.22e-32 124
MS.gene045643.t1 MTR_7g086780 31.378 341 133 12 129 379 93 422 7.24e-30 119
MS.gene045643.t1 MTR_2g016030 70.769 65 19 0 124 188 36 100 9.52e-30 112
MS.gene045643.t1 MTR_6g027450 54.167 96 38 2 99 188 47 142 6.05e-28 112
MS.gene045643.t1 MTR_8g079060 63.492 63 23 0 135 197 29 91 6.00e-23 96.3
MS.gene045643.t1 MTR_4g022370 51.429 70 34 0 119 188 66 135 1.36e-21 95.1
MS.gene045643.t1 MTR_8g027295 56.250 64 28 0 125 188 14 77 3.67e-21 92.4
MS.gene045643.t1 MTR_2g093220 57.353 68 29 0 121 188 26 93 5.90e-21 92.4
MS.gene045643.t1 MTR_3g091820 49.398 83 38 1 109 191 21 99 7.62e-21 92.0
MS.gene045643.t1 MTR_8g479350 69.231 52 16 0 137 188 70 121 9.37e-21 92.4
MS.gene045643.t1 MTR_2g096740 67.308 52 17 0 137 188 79 130 9.68e-21 92.0
MS.gene045643.t1 MTR_2g096740 67.308 52 17 0 137 188 64 115 9.74e-21 91.7
MS.gene045643.t1 MTR_4g089095 64.151 53 19 0 137 189 52 104 1.51e-20 91.3
MS.gene045643.t1 MTR_7g024670 49.412 85 34 2 103 187 54 129 1.67e-20 92.4
MS.gene045643.t1 MTR_4g088580 71.154 52 15 0 137 188 57 108 1.73e-20 92.4
MS.gene045643.t1 MTR_4g089095 64.151 53 19 0 137 189 18 70 1.74e-20 90.5
MS.gene045643.t1 MTR_8g015840 67.308 52 17 0 135 186 20 71 2.35e-20 89.0
MS.gene045643.t1 MTR_4g461080 61.538 65 21 1 127 187 17 81 2.41e-20 90.1
MS.gene045643.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 2.47e-20 90.1
MS.gene045643.t1 MTR_4g109980 64.151 53 19 0 133 185 38 90 2.48e-20 90.9
MS.gene045643.t1 MTR_1g077600 64.151 53 19 0 135 187 43 95 2.92e-20 90.1
MS.gene045643.t1 MTR_1g056810 52.941 85 37 3 125 208 1 83 3.81e-20 89.7
MS.gene045643.t1 MTR_7g059400 51.429 70 34 0 119 188 60 129 4.61e-20 90.5
MS.gene045643.t1 MTR_4g063780 43.269 104 58 1 119 221 59 162 4.85e-20 90.5
MS.gene045643.t1 MTR_2g013370 66.071 56 18 1 133 188 44 98 5.61e-20 89.4
MS.gene045643.t1 MTR_8g068210 51.389 72 27 1 117 188 22 85 9.52e-20 89.7
MS.gene045643.t1 MTR_3g090430 63.158 57 20 1 133 188 48 104 1.48e-19 89.0
MS.gene045643.t1 MTR_2g059540 67.347 49 16 0 135 183 31 79 1.72e-19 86.3
MS.gene045643.t1 MTR_5g031440 60.000 55 22 0 131 185 39 93 1.38e-18 86.3
MS.gene045643.t1 MTR_2g014170 67.347 49 16 0 135 183 45 93 1.40e-18 85.9
MS.gene045643.t1 MTR_2g014060 66.000 50 17 0 137 186 27 76 2.11e-18 85.9
MS.gene045643.t1 MTR_3g077750 64.706 51 18 0 135 185 61 111 5.91e-18 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045643.t1 AT5G62430 44.599 287 133 9 106 379 25 298 4.42e-62 201
MS.gene045643.t1 AT3G47500 33.678 484 175 18 2 379 5 448 3.67e-53 182
MS.gene045643.t1 AT5G39660 32.900 462 213 16 4 377 3 455 1.41e-49 173
MS.gene045643.t1 AT5G39660 32.900 462 213 16 4 377 3 455 1.41e-49 173
MS.gene045643.t1 AT5G39660 32.900 462 213 16 4 377 3 455 1.41e-49 173
MS.gene045643.t1 AT1G69570 35.542 332 159 14 88 379 81 397 3.04e-44 157
MS.gene045643.t1 AT1G26790 33.766 308 165 10 100 379 76 372 8.96e-41 148
MS.gene045643.t1 AT1G29160 71.212 66 19 0 123 188 50 115 6.41e-32 119
MS.gene045643.t1 AT2G34140 58.333 84 32 1 108 188 28 111 3.08e-30 114
MS.gene045643.t1 AT3G55370 35.821 134 75 1 57 190 9 131 6.06e-23 98.6
MS.gene045643.t1 AT3G55370 35.821 134 75 1 57 190 9 131 9.73e-23 98.6
MS.gene045643.t1 AT1G51700 51.190 84 31 1 111 194 19 92 5.68e-22 92.8
MS.gene045643.t1 AT5G60200 57.143 70 30 0 125 194 45 114 8.67e-22 94.0
MS.gene045643.t1 AT3G55370 47.126 87 45 1 105 190 90 176 1.05e-21 95.9
MS.gene045643.t1 AT3G50410 71.154 52 15 0 135 186 30 81 1.30e-21 93.2
MS.gene045643.t1 AT3G21270 72.549 51 14 0 137 187 31 81 3.90e-21 90.9
MS.gene045643.t1 AT3G61850 42.157 102 59 0 90 191 17 118 4.62e-21 92.4
MS.gene045643.t1 AT3G61850 42.157 102 59 0 90 191 17 118 4.62e-21 92.4
MS.gene045643.t1 AT3G61850 42.157 102 59 0 90 191 29 130 4.64e-21 92.8
MS.gene045643.t1 AT3G61850 42.157 102 59 0 90 191 17 118 6.30e-21 92.8
MS.gene045643.t1 AT3G61850 42.157 102 59 0 90 191 29 130 6.74e-21 92.8
MS.gene045643.t1 AT2G28510 67.241 58 19 0 137 194 50 107 8.89e-21 91.7
MS.gene045643.t1 AT5G02460 52.857 70 33 0 119 188 79 148 9.54e-21 93.2
MS.gene045643.t1 AT2G28810 45.679 81 44 0 106 186 48 128 1.46e-20 91.7
MS.gene045643.t1 AT5G60850 66.071 56 19 0 135 190 51 106 1.47e-20 91.7
MS.gene045643.t1 AT2G28810 45.679 81 44 0 106 186 65 145 1.85e-20 91.7
MS.gene045643.t1 AT5G66940 70.588 51 15 0 137 187 34 84 2.24e-20 89.4
MS.gene045643.t1 AT5G65590 49.425 87 41 1 104 190 13 96 2.69e-20 90.9
MS.gene045643.t1 AT1G28310 66.667 54 18 0 135 188 27 80 3.63e-20 90.5
MS.gene045643.t1 AT4G24060 63.158 57 21 0 132 188 50 106 4.02e-20 90.9
MS.gene045643.t1 AT3G45610 59.016 61 25 0 131 191 36 96 4.27e-20 89.0
MS.gene045643.t1 AT1G07640 68.627 51 16 0 137 187 23 73 4.40e-20 89.7
MS.gene045643.t1 AT1G28310 66.667 54 18 0 135 188 51 104 5.04e-20 90.5
MS.gene045643.t1 AT1G07640 68.627 51 16 0 137 187 79 129 5.09e-20 90.5
MS.gene045643.t1 AT2G37590 58.621 58 24 0 131 188 85 142 5.23e-20 90.1
MS.gene045643.t1 AT1G07640 68.627 51 16 0 137 187 87 137 6.17e-20 90.1
MS.gene045643.t1 AT1G47655 69.388 49 15 0 135 183 29 77 1.49e-19 86.7
MS.gene045643.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.45e-19 87.0
MS.gene045643.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.45e-19 87.0
MS.gene045643.t1 AT4G00940 64.286 56 20 0 137 192 70 125 4.45e-19 87.0
MS.gene045643.t1 AT4G38000 56.250 64 27 1 130 192 36 99 6.00e-19 85.9
MS.gene045643.t1 AT1G64620 51.471 68 29 1 116 183 34 97 6.10e-19 87.4
MS.gene045643.t1 AT1G21340 49.398 83 39 1 118 200 21 100 6.15e-19 85.9
MS.gene045643.t1 AT3G52440 61.404 57 21 1 133 188 22 78 8.78e-19 85.1
MS.gene045643.t1 AT3G52440 61.404 57 21 1 133 188 41 97 1.20e-18 85.5
MS.gene045643.t1 AT5G62940 67.347 49 16 0 137 185 75 123 6.31e-18 84.7
MS.gene045643.t1 AT2G46590 59.615 52 21 0 131 182 64 115 2.54e-17 82.8
MS.gene045643.t1 AT2G46590 59.615 52 21 0 131 182 76 127 2.58e-17 82.8
MS.gene045643.t1 AT4G21050 50.725 69 32 1 120 188 11 77 4.09e-17 79.7
MS.gene045643.t1 AT4G21040 45.679 81 40 2 137 216 27 104 3.32e-15 74.7
MS.gene045643.t1 AT4G21080 55.769 52 23 0 137 188 27 78 1.52e-14 73.2

Find 71 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATGGATTTGCATCTA+TGG 0.224783 5.3:+29029333 MS.gene045643:CDS
GTTCCAAGTAAACCTACTTT+AGG 0.234419 5.3:+29029160 MS.gene045643:CDS
AAACCCTTTGCATCTTCATT+AGG 0.263138 5.3:-29029271 None:intergenic
TCTTCAATTCTCAATTTCTT+AGG 0.264262 5.3:-29029247 None:intergenic
GCAAAAGCTGCCAGAGATAT+TGG 0.290195 5.3:+29028798 MS.gene045643:CDS
ACCACCATAGTATGCTATAT+TGG 0.313954 5.3:-29029089 None:intergenic
AACCCTTTGCATCTTCATTA+GGG 0.318049 5.3:-29029270 None:intergenic
CTTGTCAAGAAAATCAATAT+TGG 0.352841 5.3:+29028971 MS.gene045643:CDS
TGCCAGAGATATTGGACTTC+CGG 0.368326 5.3:+29028806 MS.gene045643:CDS
TTCCAAGTAAACCTACTTTA+GGG 0.369270 5.3:+29029161 MS.gene045643:CDS
ATGAGTAGTGTCTTGACCTT+TGG 0.369998 5.3:+29028917 MS.gene045643:CDS
TTCCCTAAAGTAGGTTTACT+TGG 0.378626 5.3:-29029163 None:intergenic
AGTGAAGATGAAGACATAAT+AGG 0.382456 5.3:-29028950 None:intergenic
ACATGATTCAAAGAGATATA+AGG 0.401349 5.3:+29027581 MS.gene045643:CDS
CAACCCAATATAGCATACTA+TGG 0.408858 5.3:+29029085 MS.gene045643:CDS
CCACCATAGTATGCTATATT+GGG 0.415830 5.3:-29029088 None:intergenic
CCTTGATAATGCTGCAGGAT+TGG 0.416917 5.3:-29029404 None:intergenic
ATCAATTTCAGGAACAATCA+AGG 0.419402 5.3:+29028498 MS.gene045643:intron
TTCAATGGATTTGCATCTAT+GGG 0.443267 5.3:+29029334 MS.gene045643:CDS
TTGCATCTATGGGTGGTGAT+AGG 0.444216 5.3:+29029344 MS.gene045643:CDS
ACACAGGATAACACATTGTT+GGG 0.445576 5.3:-29029057 None:intergenic
TAACACATTGTTGGGTTCCA+TGG 0.452271 5.3:-29029049 None:intergenic
GACACAGGATAACACATTGT+TGG 0.452547 5.3:-29029058 None:intergenic
CTTGAATGTTTCCCTAAAGT+AGG 0.465584 5.3:-29029172 None:intergenic
CTCCGGAAGTCCAATATCTC+TGG 0.468706 5.3:-29028808 None:intergenic
CTCAATAAGGCTTGAAGATG+TGG 0.477034 5.3:-29028591 None:intergenic
CATACTTCTCGTGGTTCCTC+CGG 0.479008 5.3:-29028825 None:intergenic
GCAGAAGTGACGGGGCTGGT+TGG 0.486295 5.3:-29028777 None:intergenic
TTTGAAGAATTCACTTCAAG+TGG 0.499476 5.3:-29027555 None:intergenic
GTGGTTGTTTAGTCCCATCA+TGG 0.499829 5.3:+29029107 MS.gene045643:CDS
GAAGTGTCTGTTTCTGATGA+GGG 0.503461 5.3:-29028626 None:intergenic
TATATCTCTTTGAATCATGT+TGG 0.511854 5.3:-29027578 None:intergenic
TTCCCAATCAAATCCTCTCA+TGG 0.512251 5.3:-29029438 None:intergenic
CCAATCCTGCAGCATTATCA+AGG 0.523964 5.3:+29029404 MS.gene045643:CDS
TGATAATCAAGTACTGAGGT+AGG 0.528696 5.3:-29028527 None:intergenic
AGAAGTATGCTTGTAGGTGC+AGG 0.528984 5.3:+29028839 MS.gene045643:CDS
TTAGGGAAACATTCAAGAGA+TGG 0.534355 5.3:+29029178 MS.gene045643:CDS
AGAAGTGTCTGTTTCTGATG+AGG 0.537046 5.3:-29028627 None:intergenic
TAGGTTGTACGCTCCATGAT+GGG 0.544678 5.3:-29029120 None:intergenic
AGTGTCTGTTTCTGATGAGG+GGG 0.550139 5.3:-29028624 None:intergenic
TGAGACCTTGATAATGCTGC+AGG 0.554542 5.3:-29029409 None:intergenic
CATAGTATGCTATATTGGGT+TGG 0.557895 5.3:-29029084 None:intergenic
GAGAAATCTTGATGAGCAAG+AGG 0.571155 5.3:-29027495 None:intergenic
GACCCTAATGAAGATGCAAA+GGG 0.573223 5.3:+29029268 MS.gene045643:CDS
CTAGGAAACAAGAGTGGTGG+AGG 0.573390 5.3:+29029307 MS.gene045643:CDS
ACCACGAGAAGTATGCTTGT+AGG 0.578460 5.3:+29028833 MS.gene045643:CDS
ATAGGTTGTACGCTCCATGA+TGG 0.581466 5.3:-29029121 None:intergenic
AATAAGGCTTGAAGATGTGG+TGG 0.584261 5.3:-29028588 None:intergenic
GGGTTCCATGGAAACTGTTG+TGG 0.587185 5.3:-29029037 None:intergenic
AGACCCTAATGAAGATGCAA+AGG 0.590368 5.3:+29029267 MS.gene045643:CDS
GGTCTCATGTGTTCCATGAG+AGG 0.592053 5.3:+29029425 MS.gene045643:CDS
AAGTGTCTGTTTCTGATGAG+GGG 0.596442 5.3:-29028625 None:intergenic
ACACAAGATTGAGTTGCGAT+AGG 0.596525 5.3:-29029139 None:intergenic
AATAGGAGAATCTGATCCAA+AGG 0.598047 5.3:-29028933 None:intergenic
TTGACGATTATGTGAGACAT+CGG 0.603312 5.3:-29028894 None:intergenic
ATTGGCTTTCAATACTGATG+AGG 0.608635 5.3:-29029386 None:intergenic
ATAAGGCTTGAAGATGTGGT+GGG 0.622284 5.3:-29028587 None:intergenic
TGAACAAAGTGATATGTCAC+AGG 0.628815 5.3:+29028673 MS.gene045643:CDS
AATGGATTTGCATCTATGGG+TGG 0.631829 5.3:+29029337 MS.gene045643:CDS
ACCTACAAGCATACTTCTCG+TGG 0.650658 5.3:-29028834 None:intergenic
GGGAGTGTTTAAATCCTCTG+TGG 0.652740 5.3:-29028567 None:intergenic
CCCAATATAGCATACTATGG+TGG 0.653975 5.3:+29029088 MS.gene045643:CDS
ACACTAGGAAACAAGAGTGG+TGG 0.656394 5.3:+29029304 MS.gene045643:CDS
AAATCTTGATGAGCAAGAGG+AGG 0.659290 5.3:-29027492 None:intergenic
ATATTGGGTTGGAATGACAC+AGG 0.664359 5.3:-29029073 None:intergenic
CAGAGATATTGGACTTCCGG+AGG 0.693631 5.3:+29028809 MS.gene045643:CDS
GGGTGGTGATAGGAATCATG+TGG 0.704890 5.3:+29029354 MS.gene045643:CDS
TTCTTGATAATCAAGTACTG+AGG 0.713203 5.3:-29028531 None:intergenic
AAGAGGAGGAGAAAACTCAG+AGG 0.721417 5.3:-29027478 None:intergenic
TTAACACTAGGAAACAAGAG+TGG 0.767808 5.3:+29029301 MS.gene045643:CDS
AGCCTCAAAACAAACCACAG+AGG 0.778500 5.3:+29028553 MS.gene045643:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTACTTATAGTT+AGG + chr5.3:29028142-29028161 MS.gene045643:intron 10.0%
!!! AACTATAAGTAATTAATTTT+AGG - chr5.3:29028142-29028161 None:intergenic 10.0%
!!! CTTTTTTTGTTTTAAAATTA+AGG + chr5.3:29027627-29027646 MS.gene045643:intron 10.0%
!!! TGAAAAATATACAATTTTTT+TGG + chr5.3:29028317-29028336 MS.gene045643:intron 10.0%
!! AAGTTATTAATGTTACAATT+TGG - chr5.3:29028065-29028084 None:intergenic 15.0%
!! AATTATTGTTTATCAATTTC+AGG + chr5.3:29028487-29028506 MS.gene045643:intron 15.0%
!! ATTGTAACATTAATAACTTA+TGG + chr5.3:29028066-29028085 MS.gene045643:intron 15.0%
!! TGATAAACAATAATTAGATT+AGG - chr5.3:29028482-29028501 None:intergenic 15.0%
!! TTGTAACATTAATAACTTAT+GGG + chr5.3:29028067-29028086 MS.gene045643:intron 15.0%
!! AAGTTATTAACGTTACAATT+TGG + chr5.3:29027704-29027723 MS.gene045643:intron 20.0%
!! ATAGACTTCAAAATATGAAT+TGG - chr5.3:29028398-29028417 None:intergenic 20.0%
!! ATTGTAACGTTAATAACTTA+TGG - chr5.3:29027703-29027722 None:intergenic 20.0%
!! GAAACCAAAAAATAAGTTAA+GGG + chr5.3:29027916-29027935 MS.gene045643:intron 20.0%
!! GAAACCAAAAAATAAGTTAA+GGG - chr5.3:29027935-29027916 None:intergenic 20.0%
!! TTAAGGCTTAATTAATGATT+TGG + chr5.3:29027644-29027663 MS.gene045643:intron 20.0%
!! TTGTAACGTTAATAACTTAT+GGG - chr5.3:29027702-29027721 None:intergenic 20.0%
!!! AACGAAACTTTTAATAGTTA+AGG + chr5.3:29028006-29028025 MS.gene045643:intron 20.0%
!!! AACGAAACTTTTAATAGTTA+AGG - chr5.3:29028025-29028006 None:intergenic 20.0%
!!! AACTATTAAAAGTTTCGTTT+TGG + chr5.3:29027764-29027783 MS.gene045643:intron 20.0%
!!! AACTATTAAAAGTTTCGTTT+TGG - chr5.3:29027783-29027764 None:intergenic 20.0%
!!! AAGAAGAGATTTTATTCTTA+CGG - chr5.3:29028868-29028887 None:intergenic 20.0%
!!! ACGAAACTTTTAATAGTTAA+GGG + chr5.3:29028007-29028026 MS.gene045643:intron 20.0%
!!! ACGAAACTTTTAATAGTTAA+GGG - chr5.3:29028026-29028007 None:intergenic 20.0%
!!! TTTTGGTGATAAAATTCTTA+TGG + chr5.3:29028271-29028290 MS.gene045643:intron 20.0%
! ACATGATTCAAAGAGATATA+AGG + chr5.3:29027581-29027600 MS.gene045643:CDS 25.0%
! CGAAACCAAAAAATAAGTTA+AGG + chr5.3:29027915-29027934 MS.gene045643:intron 25.0%
! CGAAACCAAAAAATAAGTTA+AGG - chr5.3:29027934-29027915 None:intergenic 25.0%
! CTTGTCAAGAAAATCAATAT+TGG + chr5.3:29028971-29028990 MS.gene045643:CDS 25.0%
! GAAACCAAAAAGTAACTTAA+GGG - chr5.3:29028057-29028038 None:intergenic 25.0%
! GGAAACCAAAAAATAAGTTA+AGG + chr5.3:29027946-29027965 MS.gene045643:intron 25.0%
! GTAACCAACAAATAACTTAA+GGG + chr5.3:29028098-29028117 MS.gene045643:intron 25.0%
! GTAACCAACAAATAACTTAA+GGG - chr5.3:29028117-29028098 None:intergenic 25.0%
! TATATCTCTTTGAATCATGT+TGG - chr5.3:29027581-29027600 None:intergenic 25.0%
! TCTTCAATTCTCAATTTCTT+AGG - chr5.3:29029250-29029269 None:intergenic 25.0%
! TGTAACCAACAAATAACTTA+AGG + chr5.3:29028097-29028116 MS.gene045643:intron 25.0%
! TGTAACCAACAAATAACTTA+AGG - chr5.3:29028116-29028097 None:intergenic 25.0%
!! ATTCAACCATTAAGCTTTTT+GGG + chr5.3:29027418-29027437 MS.gene045643:CDS 25.0%
!! GTTGCAAATAAAACTAGTTT+AGG - chr5.3:29028424-29028443 None:intergenic 25.0%
!! TAACTTTGTGGTTCATATTT+TGG + chr5.3:29028451-29028470 MS.gene045643:intron 25.0%
!! TTTAGGCTTAATTACTCTTT+TGG - chr5.3:29028125-29028144 None:intergenic 25.0%
!!! CTTATTTTTTGGTTTCCTTT+TGG - chr5.3:29027943-29027962 None:intergenic 25.0%
!!! CTTATTTTTTGGTTTCGTTT+TGG + chr5.3:29027795-29027814 MS.gene045643:intron 25.0%
!!! CTTATTTTTTGGTTTCGTTT+TGG - chr5.3:29027814-29027795 None:intergenic 25.0%
!!! GTTATTTGTTGGTTACATTT+TGG + chr5.3:29027675-29027694 MS.gene045643:intron 25.0%
!!! GTTATTTGTTGGTTACATTT+TGG - chr5.3:29027694-29027675 None:intergenic 25.0%
AAATAAGTTAAGGGACCAAA+AGG + chr5.3:29027894-29027913 MS.gene045643:intron 30.0%
AGTGAAGATGAAGACATAAT+AGG - chr5.3:29028953-29028972 None:intergenic 30.0%
ATCAATTTCAGGAACAATCA+AGG + chr5.3:29028498-29028517 MS.gene045643:intron 30.0%
CCCTCCAAAAAATAAGTTAA+GGG + chr5.3:29027885-29027904 MS.gene045643:intron 30.0%
CGAAACCAAAAAGTAACTTA+AGG - chr5.3:29028056-29028037 None:intergenic 30.0%
TCCCTCCAAAAAATAAGTTA+AGG + chr5.3:29027884-29027903 MS.gene045643:intron 30.0%
TTCCAAGTAAACCTACTTTA+GGG + chr5.3:29029161-29029180 MS.gene045643:CDS 30.0%
TTTGAAGAATTCACTTCAAG+TGG - chr5.3:29027558-29027577 None:intergenic 30.0%
! GATTCAACCATTAAGCTTTT+TGG + chr5.3:29027417-29027436 MS.gene045643:CDS 30.0%
! TTCAATGGATTTGCATCTAT+GGG + chr5.3:29029334-29029353 MS.gene045643:CDS 30.0%
! TTCTTGATAATCAAGTACTG+AGG - chr5.3:29028534-29028553 None:intergenic 30.0%
! TTTCAATGGATTTGCATCTA+TGG + chr5.3:29029333-29029352 MS.gene045643:CDS 30.0%
!! AAGATGCAAAGGGTTTTAAT+TGG + chr5.3:29029278-29029297 MS.gene045643:CDS 30.0%
!!! CACTCCCTTAACTTATTTTT+TGG + chr5.3:29027850-29027869 MS.gene045643:intron 30.0%
!!! CCCTTAACTTATTTTTTGGA+GGG - chr5.3:29027888-29027907 None:intergenic 30.0%
!!! GGTTTTAATTGGTTAACACT+AGG + chr5.3:29029289-29029308 MS.gene045643:CDS 30.0%
!!! GTGCACTTTTTTCATCTTTT+TGG + chr5.3:29028254-29028273 MS.gene045643:intron 30.0%
!!! GTTACTTTTTGGTTTCGTTT+TGG + chr5.3:29027735-29027754 MS.gene045643:intron 30.0%
!!! GTTACTTTTTGGTTTCGTTT+TGG - chr5.3:29027754-29027735 None:intergenic 30.0%
!!! TCCCTTAACTTATTTTTTGG+AGG - chr5.3:29027806-29027787 None:intergenic 30.0%
!!! TGGTCCCTTAACTTATTTTT+TGG + chr5.3:29027784-29027803 MS.gene045643:intron 30.0%
!!! TGGTCCCTTAACTTATTTTT+TGG - chr5.3:29027803-29027784 None:intergenic 30.0%
!!! TGGTCTCTTAAGTTACTTTT+TGG - chr5.3:29028045-29028064 None:intergenic 30.0%
AAACCCTTTGCATCTTCATT+AGG - chr5.3:29029274-29029293 None:intergenic 35.0%
AACCCTTTGCATCTTCATTA+GGG - chr5.3:29029273-29029292 None:intergenic 35.0%
AATAGGAGAATCTGATCCAA+AGG - chr5.3:29028936-29028955 None:intergenic 35.0%
ACACAGGATAACACATTGTT+GGG - chr5.3:29029060-29029079 None:intergenic 35.0%
ACCACCATAGTATGCTATAT+TGG - chr5.3:29029092-29029111 None:intergenic 35.0%
CAACCCAATATAGCATACTA+TGG + chr5.3:29029085-29029104 MS.gene045643:CDS 35.0%
CATAGTATGCTATATTGGGT+TGG - chr5.3:29029087-29029106 None:intergenic 35.0%
CCACCATAGTATGCTATATT+GGG - chr5.3:29029091-29029110 None:intergenic 35.0%
CTTGAATGTTTCCCTAAAGT+AGG - chr5.3:29029175-29029194 None:intergenic 35.0%
GCAACTTCAACTTAACTTTG+TGG + chr5.3:29028439-29028458 MS.gene045643:intron 35.0%
GTTCCAAGTAAACCTACTTT+AGG + chr5.3:29029160-29029179 MS.gene045643:CDS 35.0%
TAAGTTTCGTTGCAATCTTG+TGG + chr5.3:29028188-29028207 MS.gene045643:intron 35.0%
TGAACAAAGTGATATGTCAC+AGG + chr5.3:29028673-29028692 MS.gene045643:CDS 35.0%
TGGTCCCTTAAGTTATTTGT+TGG + chr5.3:29027664-29027683 MS.gene045643:intron 35.0%
TGGTCCCTTAAGTTATTTGT+TGG - chr5.3:29027683-29027664 None:intergenic 35.0%
TGTTCTCCCAAAAAGCTTAA+TGG - chr5.3:29027427-29027446 None:intergenic 35.0%
TTAACACTAGGAAACAAGAG+TGG + chr5.3:29029301-29029320 MS.gene045643:CDS 35.0%
TTAGGGAAACATTCAAGAGA+TGG + chr5.3:29029178-29029197 MS.gene045643:CDS 35.0%
TTCCCTAAAGTAGGTTTACT+TGG - chr5.3:29029166-29029185 None:intergenic 35.0%
TTGACGATTATGTGAGACAT+CGG - chr5.3:29028897-29028916 None:intergenic 35.0%
! GGACATGGAACAATTTTGTT+AGG - chr5.3:29028707-29028726 None:intergenic 35.0%
! TTTTTCCACAACAGTTTCCA+TGG + chr5.3:29029032-29029051 MS.gene045643:CDS 35.0%
!! ATTGGCTTTCAATACTGATG+AGG - chr5.3:29029389-29029408 None:intergenic 35.0%
!! TGATAATCAAGTACTGAGGT+AGG - chr5.3:29028530-29028549 None:intergenic 35.0%
!!! TGGTCCCTTAAGTTACTTTT+TGG + chr5.3:29027724-29027743 MS.gene045643:intron 35.0%
AAATCTTGATGAGCAAGAGG+AGG - chr5.3:29027495-29027514 None:intergenic 40.0%
AATAAGGCTTGAAGATGTGG+TGG - chr5.3:29028591-29028610 None:intergenic 40.0%
AGACCCTAATGAAGATGCAA+AGG + chr5.3:29029267-29029286 MS.gene045643:CDS 40.0%
ATAAGGCTTGAAGATGTGGT+GGG - chr5.3:29028590-29028609 None:intergenic 40.0%
ATGAGTAGTGTCTTGACCTT+TGG + chr5.3:29028917-29028936 MS.gene045643:CDS 40.0%
CCCAATATAGCATACTATGG+TGG + chr5.3:29029088-29029107 MS.gene045643:CDS 40.0%
CTCAATAAGGCTTGAAGATG+TGG - chr5.3:29028594-29028613 None:intergenic 40.0%
GACACAGGATAACACATTGT+TGG - chr5.3:29029061-29029080 None:intergenic 40.0%
GACCCTAATGAAGATGCAAA+GGG + chr5.3:29029268-29029287 MS.gene045643:CDS 40.0%
GAGAAATCTTGATGAGCAAG+AGG - chr5.3:29027498-29027517 None:intergenic 40.0%
TAACACATTGTTGGGTTCCA+TGG - chr5.3:29029052-29029071 None:intergenic 40.0%
! AATGGATTTGCATCTATGGG+TGG + chr5.3:29029337-29029356 MS.gene045643:CDS 40.0%
! ACACAAGATTGAGTTGCGAT+AGG - chr5.3:29029142-29029161 None:intergenic 40.0%
! TTTCTGATGAGGGGGTTTTA+GGG - chr5.3:29028619-29028638 None:intergenic 40.0%
!! AAGTGTCTGTTTCTGATGAG+GGG - chr5.3:29028628-29028647 None:intergenic 40.0%
!! AGAAGTGTCTGTTTCTGATG+AGG - chr5.3:29028630-29028649 None:intergenic 40.0%
!! ATATTGGGTTGGAATGACAC+AGG - chr5.3:29029076-29029095 None:intergenic 40.0%
!! GAAGTGTCTGTTTCTGATGA+GGG - chr5.3:29028629-29028648 None:intergenic 40.0%
!!! ATCCTCTGTGGTTTGTTTTG+AGG - chr5.3:29028558-29028577 None:intergenic 40.0%
!!! GGGTTTTAGGGTTCTCAATA+AGG - chr5.3:29028607-29028626 None:intergenic 40.0%
AAGAGGAGGAGAAAACTCAG+AGG - chr5.3:29027481-29027500 None:intergenic 45.0%
ACACTAGGAAACAAGAGTGG+TGG + chr5.3:29029304-29029323 MS.gene045643:CDS 45.0%
ACCTACAAGCATACTTCTCG+TGG - chr5.3:29028837-29028856 None:intergenic 45.0%
AGCCTCAAAACAAACCACAG+AGG + chr5.3:29028553-29028572 MS.gene045643:CDS 45.0%
ATAGGTTGTACGCTCCATGA+TGG - chr5.3:29029124-29029143 None:intergenic 45.0%
CCAATCCTGCAGCATTATCA+AGG + chr5.3:29029404-29029423 MS.gene045643:CDS 45.0%
GCAAAAGCTGCCAGAGATAT+TGG + chr5.3:29028798-29028817 MS.gene045643:CDS 45.0%
GTGGTTGTTTAGTCCCATCA+TGG + chr5.3:29029107-29029126 MS.gene045643:CDS 45.0%
TAGGTTGTACGCTCCATGAT+GGG - chr5.3:29029123-29029142 None:intergenic 45.0%
TGCCAGAGATATTGGACTTC+CGG + chr5.3:29028806-29028825 MS.gene045643:CDS 45.0%
TTGCATCTATGGGTGGTGAT+AGG + chr5.3:29029344-29029363 MS.gene045643:CDS 45.0%
! ACCACGAGAAGTATGCTTGT+AGG + chr5.3:29028833-29028852 MS.gene045643:CDS 45.0%
! ACGCTTTTGCATCTTGGACA+TGG - chr5.3:29028722-29028741 None:intergenic 45.0%
! AGAAGTATGCTTGTAGGTGC+AGG + chr5.3:29028839-29028858 MS.gene045643:CDS 45.0%
! GTGTCTACGCTTTTGCATCT+TGG - chr5.3:29028728-29028747 None:intergenic 45.0%
! GTTTCTGATGAGGGGGTTTT+AGG - chr5.3:29028620-29028639 None:intergenic 45.0%
!! AGTGGTGGAGGACTTTTCAA+TGG + chr5.3:29029319-29029338 MS.gene045643:CDS 45.0%
!! AGTGTCTGTTTCTGATGAGG+GGG - chr5.3:29028627-29028646 None:intergenic 45.0%
!! CCTTGATAATGCTGCAGGAT+TGG - chr5.3:29029407-29029426 None:intergenic 45.0%
!! GGGAGTGTTTAAATCCTCTG+TGG - chr5.3:29028570-29028589 None:intergenic 45.0%
!! TGAGACCTTGATAATGCTGC+AGG - chr5.3:29029412-29029431 None:intergenic 45.0%
CAGAGATATTGGACTTCCGG+AGG + chr5.3:29028809-29028828 MS.gene045643:CDS 50.0%
CATACTTCTCGTGGTTCCTC+CGG - chr5.3:29028828-29028847 None:intergenic 50.0%
CTAGGAAACAAGAGTGGTGG+AGG + chr5.3:29029307-29029326 MS.gene045643:CDS 50.0%
CTCCGGAAGTCCAATATCTC+TGG - chr5.3:29028811-29028830 None:intergenic 50.0%
GGGTGGTGATAGGAATCATG+TGG + chr5.3:29029354-29029373 MS.gene045643:CDS 50.0%
GGGTTCCATGGAAACTGTTG+TGG - chr5.3:29029040-29029059 None:intergenic 50.0%
GGTCTCATGTGTTCCATGAG+AGG + chr5.3:29029425-29029444 MS.gene045643:CDS 50.0%
! CAGCTTTTGCAGAAGTGACG+GGG - chr5.3:29028788-29028807 None:intergenic 50.0%
! GCAGCTTTTGCAGAAGTGAC+GGG - chr5.3:29028789-29028808 None:intergenic 50.0%
! GGCAGCTTTTGCAGAAGTGA+CGG - chr5.3:29028790-29028809 None:intergenic 50.0%
!! TTTTGCAGAAGTGACGGGGC+TGG - chr5.3:29028784-29028803 None:intergenic 55.0%
! GCAGAAGTGACGGGGCTGGT+TGG - chr5.3:29028780-29028799 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 29027408 29029453 29027408 ID=MS.gene045643
chr5.3 mRNA 29027408 29029453 29027408 ID=MS.gene045643.t1;Parent=MS.gene045643
chr5.3 exon 29027408 29027602 29027408 ID=MS.gene045643.t1.exon1;Parent=MS.gene045643.t1
chr5.3 CDS 29027408 29027602 29027408 ID=cds.MS.gene045643.t1;Parent=MS.gene045643.t1
chr5.3 exon 29028509 29029453 29028509 ID=MS.gene045643.t1.exon2;Parent=MS.gene045643.t1
chr5.3 CDS 29028509 29029453 29028509 ID=cds.MS.gene045643.t1;Parent=MS.gene045643.t1
Gene Sequence

>MS.gene045643

ATGACTAATGATTCAACCATTAAGCTTTTTGGGAGAACAATTTTTCAAACACACAACACTGATGATTGTTCCTCTGAGTTTTCTCCTCCTCTTGCTCATCAAGATTTCTCTGATCATAGTATTCATTCATCTTTATCTTCATCTTCTCCACTTGAAGTGAATTCTTCAAACCAACATGATTCAAAGAGATATAAGGTATGTTTCATGCTAATTTTTCATCTTTTTTTGTTTTAAAATTAAGGCTTAATTAATGATTTGGTCCCTTAAGTTATTTGTTGGTTACATTTTGGTCCCATAAGTTATTAACGTTACAATTTGGTCCCTTAAGTTACTTTTTGGTTTCGTTTTGGTCCCTTAACTATTAAAAGTTTCGTTTTGGTCCCTTAACTTATTTTTTGGTTTCGTTTTGGTCCCTTAACTTATTTTTTGGTTTCGTTTTGGTCACTCCCTTAACTTATTTTTTGGTTTCGTTTTGGTCCCTCCAAAAAATAAGTTAAGGGACCAAAACGAAACCAAAAAATAAGTTAAGGGACCAAAAGGAAACCAAAAAATAAGTTAAGGGACCAAAACGAAACCAAAAAATAAGTTAAGGGACCAAAACGAAACTTTTAATAGTTAAGGGACCAAAACGAAACCAAAAAGTAACTTAAGAGACCAAATTGTAACATTAATAACTTATGGGACCAAAATGTAACCAACAAATAACTTAAGGGACCAAAAGAGTAATTAAGCCTAAAATTAATTACTTATAGTTAGGTGTATGCAAATGTGTATTTATACTAAGTTTCGTTGCAATCTTGTGGCTATTTTGACAACTTTTTGCTTGAAACTATGCTTCATGAAATTGTGCACTTTTTTCATCTTTTTGGTGATAAAATTCTTATGGCTGTTTTGACAACTTTTTTTGCTTGAAAAATATACAATTTTTTTGGATTTCATGATGACTGATGCTTGATGAAATTGTGCTTTTAAAGTTTTCAAGTTTTTCCAATTCATATTTTGAAGTCTATAATCCTAAACTAGTTTTATTTGCAACTTCAACTTAACTTTGTGGTTCATATTTTGGTCTTACCTAATCTAATTATTGTTTATCAATTTCAGGAACAATCAAGGAAAAAACCTACCTCAGTACTTGATTATCAAGAAGCCTCAAAACAAACCACAGAGGATTTAAACACTCCCACCACATCTTCAAGCCTTATTGAGAACCCTAAAACCCCCTCATCAGAAACAGACACTTCTCAACAGAATTCTACTAAAATTGATGAACAAAGTGATATGTCACAGGACAAAGCTCCTAACAAAATTGTTCCATGTCCAAGATGCAAAAGCGTAGACACAAAATTTTGTTACTACAATAATTACAACGCCAACCAGCCCCGTCACTTCTGCAAAAGCTGCCAGAGATATTGGACTTCCGGAGGAACCACGAGAAGTATGCTTGTAGGTGCAGGTCGCCGTAAGAATAAAATCTCTTCTTTTTCTTCCGATGTCTCACATAATCGTCAAATGAGTAGTGTCTTGACCTTTGGATCAGATTCTCCTATTATGTCTTCATCTTCACTTGTCAAGAAAATCAATATTGGATCAAATGATGAAACATCTGATAAGAGTAATCAGTGTTTTTTTCCACAACAGTTTCCATGGAACCCAACAATGTGTTATCCTGTGTCATTCCAACCCAATATAGCATACTATGGTGGTTGTTTAGTCCCATCATGGAGCGTACAACCTATCGCAACTCAATCTTGTGTTCCAAGTAAACCTACTTTAGGGAAACATTCAAGAGATGGCTTAGAAAATAACAACAAAGAAAGTGATAATAATAGTGTGTTGATTCCTAAGAAATTGAGAATTGAAGACCCTAATGAAGATGCAAAGGGTTTTAATTGGTTAACACTAGGAAACAAGAGTGGTGGAGGACTTTTCAATGGATTTGCATCTATGGGTGGTGATAGGAATCATGTGGTTGAAGCAGCCTCATCAGTATTGAAAGCCAATCCTGCAGCATTATCAAGGTCTCATGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene045643.t1

MTNDSTIKLFGRTIFQTHNTDDCSSEFSPPLAHQDFSDHSIHSSLSSSSPLEVNSSNQHDSKRYKEQSRKKPTSVLDYQEASKQTTEDLNTPTTSSSLIENPKTPSSETDTSQQNSTKIDEQSDMSQDKAPNKIVPCPRCKSVDTKFCYYNNYNANQPRHFCKSCQRYWTSGGTTRSMLVGAGRRKNKISSFSSDVSHNRQMSSVLTFGSDSPIMSSSSLVKKINIGSNDETSDKSNQCFFPQQFPWNPTMCYPVSFQPNIAYYGGCLVPSWSVQPIATQSCVPSKPTLGKHSRDGLENNNKESDNNSVLIPKKLRIEDPNEDAKGFNWLTLGNKSGGGLFNGFASMGGDRNHVVEAASSVLKANPAALSRSHVFHERI