AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045645

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045645.t1 MTR_5g041350 92.635 353 26 0 1 353 1 353 0.0 615
MS.gene045645.t1 MTR_5g041350 91.635 263 22 0 91 353 2 264 1.21e-153 432
MS.gene045645.t1 MTR_5g041350 92.562 242 18 0 112 353 1 242 4.85e-141 400
MS.gene045645.t1 MTR_4g081710 61.823 351 117 6 2 350 4 339 3.45e-128 371
MS.gene045645.t1 MTR_2g086450 40.390 359 181 7 8 345 38 384 4.71e-69 221
MS.gene045645.t1 MTR_6g032990 48.936 235 113 3 8 238 35 266 2.20e-64 209
MS.gene045645.t1 MTR_6g444980 55.414 157 61 3 8 164 28 175 2.93e-49 169
MS.gene045645.t1 MTR_5g027440 44.865 185 94 4 5 186 15 194 7.29e-46 158
MS.gene045645.t1 MTR_6g444980 52.174 161 68 3 8 168 28 179 5.87e-45 157
MS.gene045645.t1 MTR_7g088070 51.034 145 62 2 14 158 49 184 1.42e-42 149
MS.gene045645.t1 MTR_2g027800 41.949 236 116 7 8 230 32 259 3.45e-42 149
MS.gene045645.t1 MTR_7g088070 51.034 145 58 3 14 158 49 180 1.12e-41 147
MS.gene045645.t1 MTR_2g027800 41.202 233 108 7 8 230 32 245 1.04e-40 145
MS.gene045645.t1 MTR_1g090670 50.000 142 68 1 8 149 37 175 9.98e-40 142
MS.gene045645.t1 MTR_2g027860 48.387 155 69 3 15 169 49 192 1.34e-38 140
MS.gene045645.t1 MTR_7g098250 44.218 147 66 3 11 155 258 390 1.83e-36 138
MS.gene045645.t1 MTR_1g080330 44.375 160 71 5 11 168 171 314 1.08e-35 134
MS.gene045645.t1 MTR_7g115530 37.766 188 93 3 15 200 218 383 1.19e-35 135
MS.gene045645.t1 MTR_1g053835 40.940 149 74 2 11 159 254 388 2.45e-32 125
MS.gene045645.t1 MTR_7g093030 35.849 159 85 2 14 170 211 354 3.96e-30 119
MS.gene045645.t1 MTR_1g080330 45.238 126 53 4 11 134 171 282 7.37e-28 110
MS.gene045645.t1 MTR_7g115530 43.548 124 54 2 14 135 217 326 6.48e-27 108
MS.gene045645.t1 MTR_7g068600 38.621 145 74 4 11 152 9 141 3.05e-24 99.8
MS.gene045645.t1 MTR_1g053800 38.255 149 73 2 8 153 170 302 5.45e-23 98.2
MS.gene045645.t1 MTR_7g115530 51.220 82 38 1 14 93 217 298 7.44e-22 94.7
MS.gene045645.t1 MTR_7g115530 58.621 58 24 0 14 71 217 274 1.04e-20 92.0
MS.gene045645.t1 MTR_7g093030 55.172 58 26 0 14 71 211 268 3.39e-19 87.0
MS.gene045645.t1 MTR_7g115530 61.538 52 20 0 15 66 218 269 3.45e-19 86.7
MS.gene045645.t1 MTR_1g053830 49.180 61 31 0 11 71 14 74 5.78e-19 81.3
MS.gene045645.t1 MTR_7g115530 58.491 53 22 0 15 67 218 270 2.42e-18 84.7
MS.gene045645.t1 MTR_1g112370 47.778 90 37 3 16 100 23 107 1.57e-17 82.8
MS.gene045645.t1 MTR_3g450310 46.067 89 45 2 15 100 19 107 2.89e-17 79.7
MS.gene045645.t1 MTR_0223s0040 50.000 76 35 1 16 88 19 94 7.44e-17 80.1
MS.gene045645.t1 MTR_1g090670 43.689 103 55 1 47 149 1 100 9.63e-17 78.6
MS.gene045645.t1 MTR_7g069660 56.604 53 23 0 16 68 72 124 1.57e-16 80.1
MS.gene045645.t1 MTR_2g023580 51.667 60 29 0 16 75 56 115 2.35e-16 77.8
MS.gene045645.t1 MTR_4g021790 58.182 55 22 1 15 69 168 221 5.20e-16 77.4
MS.gene045645.t1 MTR_2g016220 41.304 92 45 3 7 98 83 165 1.49e-15 77.4
MS.gene045645.t1 MTR_2g016220 41.304 92 45 3 7 98 83 165 1.78e-15 77.0
MS.gene045645.t1 MTR_5g029470 44.872 78 43 0 16 93 220 297 2.93e-15 76.6
MS.gene045645.t1 MTR_6g071625 42.045 88 50 1 16 102 203 290 3.09e-15 76.3
MS.gene045645.t1 MTR_2g016220 38.710 93 50 3 7 98 83 169 6.34e-15 75.5
MS.gene045645.t1 MTR_8g086410 52.830 53 25 0 16 68 112 164 7.26e-15 75.9
MS.gene045645.t1 MTR_8g086410 52.830 53 25 0 16 68 110 162 7.27e-15 75.5
MS.gene045645.t1 MTR_2g084230 52.830 53 25 0 16 68 98 150 1.13e-14 74.7
MS.gene045645.t1 MTR_4g121020 57.627 59 24 1 11 69 211 268 1.14e-14 75.5
MS.gene045645.t1 MTR_4g121020 57.627 59 24 1 11 69 211 268 1.17e-14 75.5
MS.gene045645.t1 MTR_2g084230 52.830 53 25 0 16 68 98 150 1.19e-14 73.9
MS.gene045645.t1 MTR_4g121020 57.627 59 24 1 11 69 107 164 1.24e-14 75.5
MS.gene045645.t1 MTR_4g021855 56.364 55 23 1 15 69 168 221 1.87e-14 73.6
MS.gene045645.t1 MTR_2g034960 54.286 70 25 2 11 74 207 275 2.33e-14 74.7
MS.gene045645.t1 MTR_5g017980 60.000 55 22 0 15 69 193 247 2.87e-14 73.2
MS.gene045645.t1 MTR_8g066310 45.070 71 39 0 16 86 174 244 4.17e-14 72.8
MS.gene045645.t1 MTR_8g086410 44.615 65 32 1 16 76 112 176 4.91e-14 73.2
MS.gene045645.t1 MTR_3g064840 52.632 57 27 0 16 72 275 331 8.93e-14 72.4
MS.gene045645.t1 MTR_1g032570 54.237 59 26 1 11 69 127 184 9.66e-14 72.8
MS.gene045645.t1 MTR_1g021520 39.773 88 45 1 16 95 127 214 1.08e-13 71.2
MS.gene045645.t1 MTR_1g032570 54.237 59 26 1 11 69 196 253 1.11e-13 72.4
MS.gene045645.t1 MTR_4g086835 55.172 58 26 0 12 69 271 328 1.15e-13 72.0
MS.gene045645.t1 MTR_4g021760 55.556 54 23 1 15 68 169 221 1.25e-13 71.2
MS.gene045645.t1 MTR_7g089010 56.364 55 23 1 15 69 91 144 1.78e-13 70.5
MS.gene045645.t1 MTR_2g450070 52.542 59 27 1 11 69 209 266 2.06e-13 71.6
MS.gene045645.t1 MTR_2g450070 52.542 59 27 1 11 69 209 266 2.12e-13 71.6
MS.gene045645.t1 MTR_4g064730 53.448 58 26 1 12 69 128 184 2.67e-13 70.1
MS.gene045645.t1 MTR_4g113140 53.448 58 27 0 12 69 232 289 5.69e-13 70.1
MS.gene045645.t1 MTR_1g093080 60.000 55 22 0 15 69 211 265 5.85e-13 69.7
MS.gene045645.t1 MTR_4g021845 56.863 51 21 1 15 65 168 217 6.01e-13 67.8
MS.gene045645.t1 MTR_5g017980 60.000 55 21 1 15 69 193 246 6.76e-13 68.9
MS.gene045645.t1 MTR_5g017980 60.000 55 21 1 15 69 192 245 7.30e-13 68.9
MS.gene045645.t1 MTR_4g086835 55.172 58 25 1 12 69 271 327 1.64e-12 68.6
MS.gene045645.t1 MTR_7g093010 32.609 138 80 3 14 150 212 337 1.70e-12 68.2
MS.gene045645.t1 MTR_7g026400 52.727 55 25 1 15 69 157 210 1.87e-11 65.5
MS.gene045645.t1 MTR_5g054300 54.545 55 25 0 15 69 198 252 2.19e-11 64.7
MS.gene045645.t1 MTR_4g131600 49.153 59 29 1 11 69 197 254 2.19e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045645.t1 AT1G69580 50.562 267 114 5 6 263 25 282 1.03e-75 237
MS.gene045645.t1 AT1G69580 50.000 268 115 5 6 263 25 283 1.84e-74 234
MS.gene045645.t1 AT3G04030 54.113 231 96 4 8 233 38 263 3.28e-68 219
MS.gene045645.t1 AT5G18240 52.174 230 100 4 8 232 38 262 1.94e-66 215
MS.gene045645.t1 AT5G18240 52.174 230 100 4 8 232 38 262 1.94e-66 215
MS.gene045645.t1 AT3G04030 53.247 231 97 5 8 233 38 262 3.41e-65 211
MS.gene045645.t1 AT5G18240 51.304 230 100 5 8 232 38 260 4.23e-63 206
MS.gene045645.t1 AT3G04030 51.948 231 95 5 8 233 38 257 1.91e-61 202
MS.gene045645.t1 AT5G18240 50.000 230 99 5 8 232 38 256 1.27e-59 197
MS.gene045645.t1 AT5G18240 50.000 230 99 5 8 232 38 256 1.27e-59 197
MS.gene045645.t1 AT4G13640 46.154 234 104 7 8 229 30 253 5.50e-57 187
MS.gene045645.t1 AT3G24120 45.575 226 115 4 8 226 34 258 8.17e-57 187
MS.gene045645.t1 AT4G13640 45.570 237 104 8 8 229 30 256 8.42e-55 182
MS.gene045645.t1 AT3G24120 44.978 229 115 5 8 226 34 261 1.21e-54 181
MS.gene045645.t1 AT1G79430 56.688 157 59 3 8 164 27 174 2.84e-51 174
MS.gene045645.t1 AT5G45580 43.902 164 86 2 9 166 1 164 3.93e-42 148
MS.gene045645.t1 AT5G45580 45.122 164 84 2 9 166 17 180 5.30e-42 148
MS.gene045645.t1 AT3G04030 48.958 192 88 4 47 233 1 187 1.14e-40 145
MS.gene045645.t1 AT2G01060 48.701 154 67 4 14 167 14 155 1.51e-40 144
MS.gene045645.t1 AT3G12730 48.701 154 71 3 8 159 16 163 2.21e-40 142
MS.gene045645.t1 AT3G04450 43.243 148 75 2 11 158 194 332 1.33e-35 134
MS.gene045645.t1 AT3G04450 43.243 148 75 2 11 158 194 332 1.33e-35 134
MS.gene045645.t1 AT5G29000 44.000 150 65 3 14 158 187 322 1.85e-35 133
MS.gene045645.t1 AT5G29000 44.000 150 65 3 14 158 187 322 1.85e-35 133
MS.gene045645.t1 AT3G04450 43.243 148 75 2 11 158 234 372 1.89e-35 134
MS.gene045645.t1 AT5G29000 44.000 150 65 3 14 158 230 365 3.00e-35 133
MS.gene045645.t1 AT4G28610 41.892 148 75 2 14 161 224 360 1.08e-34 132
MS.gene045645.t1 AT3G04030 46.354 192 87 5 47 233 1 181 4.09e-34 128
MS.gene045645.t1 AT5G18240 45.026 191 89 5 47 232 1 180 2.75e-33 126
MS.gene045645.t1 AT5G06800 41.667 144 76 2 15 158 192 327 3.64e-32 124
MS.gene045645.t1 AT5G06800 41.667 144 71 3 15 158 192 322 2.16e-31 122
MS.gene045645.t1 AT3G13040 44.595 148 65 4 11 153 237 372 2.29e-30 120
MS.gene045645.t1 AT3G13040 44.595 148 65 4 11 153 237 372 2.29e-30 120
MS.gene045645.t1 AT3G13040 44.595 148 65 4 11 153 237 372 2.29e-30 120
MS.gene045645.t1 AT2G20400 41.830 153 68 3 15 162 231 367 1.03e-29 118
MS.gene045645.t1 AT2G20400 41.176 153 68 3 15 162 231 366 3.09e-29 116
MS.gene045645.t1 AT1G79430 52.542 118 47 3 47 164 1 109 6.73e-28 110
MS.gene045645.t1 AT3G04450 57.377 61 26 0 11 71 234 294 7.60e-21 92.0
MS.gene045645.t1 AT2G01060 44.915 118 53 4 50 167 1 106 9.39e-21 90.1
MS.gene045645.t1 AT5G29000 56.897 58 25 0 14 71 230 287 2.13e-20 90.9
MS.gene045645.t1 AT2G06020 54.098 61 28 0 11 71 82 142 2.22e-18 84.7
MS.gene045645.t1 AT5G06800 56.140 57 25 0 15 71 192 248 3.36e-18 84.0
MS.gene045645.t1 AT1G14600 58.491 53 22 0 16 68 24 76 1.42e-17 81.6
MS.gene045645.t1 AT3G12730 42.609 115 58 3 47 159 1 109 2.31e-17 79.3
MS.gene045645.t1 AT2G02060 52.459 61 29 0 16 76 30 90 7.34e-17 79.7
MS.gene045645.t1 AT2G02060 52.459 61 29 0 16 76 30 90 7.34e-17 79.7
MS.gene045645.t1 AT1G32240 44.872 78 43 0 16 93 214 291 7.10e-16 78.6
MS.gene045645.t1 AT1G32240 44.872 78 43 0 16 93 214 291 7.84e-16 78.2
MS.gene045645.t1 AT2G38300 56.604 53 23 0 16 68 55 107 1.57e-15 77.0
MS.gene045645.t1 AT4G04605 52.830 53 25 0 16 68 15 67 1.99e-15 75.1
MS.gene045645.t1 AT2G42660 50.000 64 29 1 16 79 51 111 5.27e-15 74.3
MS.gene045645.t1 AT2G40260 50.847 59 29 0 16 74 83 141 8.71e-15 75.5
MS.gene045645.t1 AT4G17695 45.570 79 37 1 16 88 165 243 1.66e-14 73.9
MS.gene045645.t1 AT5G16560 52.632 57 27 0 16 72 220 276 3.05e-14 73.6
MS.gene045645.t1 AT5G59570 53.448 58 26 1 12 69 138 194 3.38e-14 72.8
MS.gene045645.t1 AT5G59570 53.448 58 26 1 12 69 138 194 3.38e-14 72.8
MS.gene045645.t1 AT2G40970 55.172 58 25 1 12 69 102 158 5.68e-14 71.2
MS.gene045645.t1 AT5G05090 56.364 55 23 1 15 69 81 134 5.81e-14 71.6
MS.gene045645.t1 AT3G10760 56.364 55 23 1 15 69 105 158 7.76e-14 72.0
MS.gene045645.t1 AT5G42630 51.786 56 27 0 16 71 106 161 8.24e-14 70.5
MS.gene045645.t1 AT3G16857 56.364 55 23 1 15 69 167 220 9.28e-14 72.8
MS.gene045645.t1 AT3G16857 56.364 55 23 1 15 69 237 290 1.05e-13 72.8
MS.gene045645.t1 AT3G16857 56.364 55 23 1 15 69 237 290 1.12e-13 72.8
MS.gene045645.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.13e-13 71.6
MS.gene045645.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.13e-13 71.6
MS.gene045645.t1 AT5G42630 51.786 56 27 0 16 71 106 161 1.22e-13 70.9
MS.gene045645.t1 AT3G46640 54.545 55 24 1 15 69 144 197 1.35e-13 71.2
MS.gene045645.t1 AT1G25550 55.172 58 26 0 12 69 206 263 3.00e-13 70.5
MS.gene045645.t1 AT1G49560 60.000 55 22 0 15 69 192 246 3.24e-13 70.1
MS.gene045645.t1 AT1G68670 56.897 58 25 0 12 69 212 269 6.08e-13 69.7
MS.gene045645.t1 AT3G25790 49.206 63 32 0 20 82 201 263 6.29e-13 69.3
MS.gene045645.t1 AT4G04580 52.830 53 25 0 16 68 15 67 1.32e-12 65.9
MS.gene045645.t1 AT2G03500 54.545 55 25 0 15 69 233 287 2.45e-12 68.2
MS.gene045645.t1 AT4G37180 52.830 53 25 0 17 69 212 264 2.83e-12 67.4
MS.gene045645.t1 AT4G37180 52.830 53 25 0 17 69 219 271 3.33e-12 67.4
MS.gene045645.t1 AT5G58080 54.237 59 26 1 11 69 190 247 7.63e-12 67.0
MS.gene045645.t1 AT2G01760 50.909 55 26 1 15 69 135 188 8.50e-12 65.9
MS.gene045645.t1 AT2G01760 50.909 55 26 1 15 69 200 253 1.28e-11 65.9
MS.gene045645.t1 AT1G67710 45.588 68 32 1 17 79 121 188 1.54e-11 65.9
MS.gene045645.t1 AT2G01760 50.909 55 26 1 15 69 228 281 1.84e-11 65.5
MS.gene045645.t1 AT1G67710 45.588 68 32 1 17 79 195 262 2.12e-11 65.5
MS.gene045645.t1 AT1G67710 45.588 68 32 1 17 79 130 197 2.15e-11 65.1
MS.gene045645.t1 AT1G13300 46.269 67 36 0 15 81 181 247 2.32e-11 64.7
MS.gene045645.t1 AT5G45580 35.455 110 65 2 63 166 16 125 2.83e-11 63.2
MS.gene045645.t1 AT5G44190 50.000 54 26 1 15 68 147 199 4.81e-11 63.9
MS.gene045645.t1 AT3G62670 46.774 62 29 1 11 68 207 268 4.83e-11 63.9
MS.gene045645.t1 AT5G44190 50.000 54 26 1 15 68 147 199 6.36e-11 63.5
MS.gene045645.t1 AT1G49560 50.000 64 30 1 15 78 192 253 7.36e-11 62.4
MS.gene045645.t1 AT1G25550 51.852 54 26 0 12 65 206 259 9.56e-11 62.4

Find 56 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACTCAGTGATTTGATTC+TGG 0.268350 5.3:+28945602 None:intergenic
TGGAACTTCTTTGTGTTCAT+TGG 0.299674 5.3:-28944912 MS.gene045645:CDS
CAGAGTAGGTTTCTAGTTGT+TGG 0.316123 5.3:+28945947 None:intergenic
ATGTTGAGAATTATTCATTA+AGG 0.320144 5.3:-28944652 MS.gene045645:CDS
TTCACTTGAATTGCCACTAA+TGG 0.322715 5.3:-28944798 MS.gene045645:CDS
TAATTGCATCAATGAATCTT+TGG 0.326033 5.3:+28946297 None:intergenic
AGTCCATGTCATTCTGATAT+TGG 0.338286 5.3:+28944570 None:intergenic
AAACATTCATTAGAAGAAAT+AGG 0.341588 5.3:-28944845 MS.gene045645:CDS
ATGAATCTTTGGTGAAGTTC+AGG 0.353886 5.3:+28946308 None:intergenic
CCATCTCAAGAGCCATTTAC+AGG 0.357774 5.3:-28946082 MS.gene045645:intron
AATGAAGAAACTAAATTTGA+TGG 0.358160 5.3:-28944680 MS.gene045645:CDS
ATGCAAAACCAAGGCTCAAA+TGG 0.392729 5.3:-28946334 MS.gene045645:CDS
GCATTGTTCATCACAGCTCT+TGG 0.396519 5.3:+28945648 None:intergenic
AAGGCGCAGGAGGCCCTCTC+TGG 0.408389 5.3:-28945088 MS.gene045645:CDS
GGATACAATTCTTCACCAAT+TGG 0.409885 5.3:-28945067 MS.gene045645:CDS
TTGTTCTTTGTTGTCAGAGT+AGG 0.427163 5.3:+28945933 None:intergenic
AAAAGACGAACTGTCTCAAT+TGG 0.442895 5.3:-28945032 MS.gene045645:CDS
GCAGCAGAGAAATCAGTCTT+GGG 0.443255 5.3:-28945627 MS.gene045645:CDS
TCTGACCGCATGGCCATTAG+TGG 0.447992 5.3:+28944785 None:intergenic
GATTCTGTTAGATCTGAGAT+TGG 0.449789 5.3:+28944977 None:intergenic
TCAGGAGTCCATTTGAGCCT+TGG 0.464509 5.3:+28946326 None:intergenic
TCATGCTCTAGAAATGCAAA+TGG 0.465824 5.3:-28945459 MS.gene045645:CDS
TGCAGCAGAGAAATCAGTCT+TGG 0.471008 5.3:-28945628 MS.gene045645:CDS
ATTGATGCAATTAATCAACT+TGG 0.481050 5.3:-28946287 MS.gene045645:CDS
ATGAGAGTGATGGAAATTCC+AGG 0.483319 5.3:-28946117 MS.gene045645:CDS
AGATCTGAGATTGGAGAACT+TGG 0.485427 5.3:+28944986 None:intergenic
ATCACTCTCATAATACTCTT+CGG 0.486005 5.3:+28946129 None:intergenic
AATTATTCATTAAGGAAAAG+AGG 0.490541 5.3:-28944644 MS.gene045645:CDS
TTTACAATCGGTACTAATGA+AGG 0.497419 5.3:-28945107 MS.gene045645:CDS
TCAAGGAAAATATTTACAAT+CGG 0.522599 5.3:-28945119 MS.gene045645:CDS
AGATGGTACAAAGTAAGTCC+TGG 0.523577 5.3:+28946099 None:intergenic
GAAACTTAATGAACAAATTG+AGG 0.524227 5.3:-28945426 MS.gene045645:intron
GATGCAATTAATCAACTTGG+AGG 0.524466 5.3:-28946284 MS.gene045645:CDS
AAATGCAAATGGAAGTAGAA+AGG 0.543789 5.3:-28945448 MS.gene045645:CDS
CCTGTAAATGGCTCTTGAGA+TGG 0.548306 5.3:+28946082 None:intergenic
AGGGTTAAGTTTAAACTATG+AGG 0.556660 5.3:-28944951 MS.gene045645:CDS
GAAGAGTATTATGAGAGTGA+TGG 0.566590 5.3:-28946127 MS.gene045645:CDS
CGAAGCACAGATACAGACAT+TGG 0.567770 5.3:+28944743 None:intergenic
CAGCTTCGAATCGAAGCTCA+AGG 0.569311 5.3:-28945136 MS.gene045645:CDS
TGATTTCTCTTCTGACCGCA+TGG 0.575568 5.3:+28944775 None:intergenic
TAAGTTTAAACTATGAGGAG+AGG 0.577698 5.3:-28944946 MS.gene045645:CDS
ATCGGTACTAATGAAGGCGC+AGG 0.585210 5.3:-28945101 MS.gene045645:CDS
GGTTGTAGAAATATAGACTT+GGG 0.601442 5.3:-28945975 MS.gene045645:intron
GTGAAGAATTGTATCCAGAG+AGG 0.609917 5.3:+28945074 None:intergenic
TGGTTGTAGAAATATAGACT+TGG 0.620704 5.3:-28945976 MS.gene045645:intron
TCAGATCTAACAGAATCAAG+AGG 0.622910 5.3:-28944971 MS.gene045645:CDS
TGAAGAATTGTATCCAGAGA+GGG 0.628116 5.3:+28945075 None:intergenic
TACTCTGACAACAAAGAACA+AGG 0.633344 5.3:-28945931 MS.gene045645:intron
CAGATCTAACAGAATCAAGA+GGG 0.636389 5.3:-28944970 MS.gene045645:CDS
GAGGAGAGGAAACATGACAA+TGG 0.651274 5.3:-28944932 MS.gene045645:CDS
GGTACTAATGAAGGCGCAGG+AGG 0.653630 5.3:-28945098 MS.gene045645:CDS
CAGCAGAGAAATCAGTCTTG+GGG 0.657853 5.3:-28945626 MS.gene045645:CDS
TATTCATTAAGGAAAAGAGG+TGG 0.668863 5.3:-28944641 MS.gene045645:CDS
TAGCCAATATCAGAATGACA+TGG 0.678287 5.3:-28944573 MS.gene045645:CDS
TATCCACTGATGCAAAACCA+AGG 0.720964 5.3:-28946343 MS.gene045645:CDS
AATTGCCACTAATGGCCATG+CGG 0.734562 5.3:-28944790 MS.gene045645:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGAAAAAAAATAGTA+AGG + chr5.3:28944659-28944678 None:intergenic 10.0%
!! TTATAATATCATTACAAAAA+AGG + chr5.3:28944831-28944850 None:intergenic 10.0%
!!! GTAATGATATTATAATTTTT+TGG - chr5.3:28944836-28944855 MS.gene045645:CDS 10.0%
!! AATTTGAAAATAAAATCTCT+AGG + chr5.3:28945375-28945394 None:intergenic 15.0%
!!! TTTTTTTTTTACCAAAATCA+CGG + chr5.3:28945589-28945608 None:intergenic 15.0%
!! AAACATTCATTAGAAGAAAT+AGG - chr5.3:28946037-28946056 MS.gene045645:intron 20.0%
!! AATGAAGAAACTAAATTTGA+TGG - chr5.3:28946202-28946221 MS.gene045645:intron 20.0%
!! AATTATTCATTAAGGAAAAG+AGG - chr5.3:28946238-28946257 MS.gene045645:intron 20.0%
!! ATGTTGAGAATTATTCATTA+AGG - chr5.3:28946230-28946249 MS.gene045645:intron 20.0%
!! TCAAGGAAAATATTTACAAT+CGG - chr5.3:28945763-28945782 MS.gene045645:intron 20.0%
! AAAAAGGTAATACCTGTAAA+TGG + chr5.3:28944815-28944834 None:intergenic 25.0%
! AAATAGTATGATCGATCATT+TGG - chr5.3:28945171-28945190 MS.gene045645:intron 25.0%
! AAATGATCGATCATACTATT+TGG + chr5.3:28945172-28945191 None:intergenic 25.0%
! ATGTATATGTGATGAAATCA+CGG + chr5.3:28945645-28945664 None:intergenic 25.0%
! ATTGATGCAATTAATCAACT+TGG - chr5.3:28944595-28944614 MS.gene045645:CDS 25.0%
! GAAACTTAATGAACAAATTG+AGG - chr5.3:28945456-28945475 MS.gene045645:CDS 25.0%
! TAATTGCATCAATGAATCTT+TGG + chr5.3:28944588-28944607 None:intergenic 25.0%
! TCAAATTGTGTATGTTAATG+TGG - chr5.3:28944719-28944738 MS.gene045645:CDS 25.0%
!!! GACTTTTCATGTTTTTTTAC+TGG - chr5.3:28944886-28944905 MS.gene045645:CDS 25.0%
!!! TTTGTCAGTTTTATTCCAAT+TGG + chr5.3:28945833-28945852 None:intergenic 25.0%
AAATGCAAATGGAAGTAGAA+AGG - chr5.3:28945434-28945453 MS.gene045645:CDS 30.0%
AGGGTTAAGTTTAAACTATG+AGG - chr5.3:28945931-28945950 MS.gene045645:intron 30.0%
ATCACTCTCATAATACTCTT+CGG + chr5.3:28944756-28944775 None:intergenic 30.0%
ATCATTTGGCATAACATTGA+TGG - chr5.3:28945185-28945204 MS.gene045645:intron 30.0%
ATTACTCAGTGATTTGATTC+TGG + chr5.3:28945283-28945302 None:intergenic 30.0%
GGTTGTAGAAATATAGACTT+GGG - chr5.3:28944907-28944926 MS.gene045645:CDS 30.0%
TAAGGACGTGTTAATCAATT+TGG - chr5.3:28945695-28945714 MS.gene045645:intron 30.0%
TAAGTTTAAACTATGAGGAG+AGG - chr5.3:28945936-28945955 MS.gene045645:CDS 30.0%
TATGCACTAAATGTTGCATA+AGG - chr5.3:28945677-28945696 MS.gene045645:intron 30.0%
TATTCATTAAGGAAAAGAGG+TGG - chr5.3:28946241-28946260 MS.gene045645:intron 30.0%
TCAATTTGGTTGTTCATTTC+AGG - chr5.3:28945709-28945728 MS.gene045645:intron 30.0%
TGCAACATTTAGTGCATATT+TGG + chr5.3:28945675-28945694 None:intergenic 30.0%
TGGTTGTAGAAATATAGACT+TGG - chr5.3:28944906-28944925 MS.gene045645:CDS 30.0%
TTTACAATCGGTACTAATGA+AGG - chr5.3:28945775-28945794 MS.gene045645:intron 30.0%
! ACCGTTAATTTGAAATCAGA+CGG + chr5.3:28945107-28945126 None:intergenic 30.0%
! CTAACTTCCAAGTACATTTT+TGG - chr5.3:28945528-28945547 MS.gene045645:intron 30.0%
!! TTCCAAGTACATTTTTGGAT+TGG - chr5.3:28945533-28945552 MS.gene045645:intron 30.0%
AAAAGACGAACTGTCTCAAT+TGG - chr5.3:28945850-28945869 MS.gene045645:intron 35.0%
AGTCCATGTCATTCTGATAT+TGG + chr5.3:28946315-28946334 None:intergenic 35.0%
ATGAATCTTTGGTGAAGTTC+AGG + chr5.3:28944577-28944596 None:intergenic 35.0%
ATGAGATATCTCCATGTTTC+TGG + chr5.3:28945407-28945426 None:intergenic 35.0%
CAGATCTAACAGAATCAAGA+GGG - chr5.3:28945912-28945931 MS.gene045645:intron 35.0%
GATGCAATTAATCAACTTGG+AGG - chr5.3:28944598-28944617 MS.gene045645:CDS 35.0%
GCCGTCTGATTTCAAATTAA+CGG - chr5.3:28945103-28945122 MS.gene045645:CDS 35.0%
GGATACAATTCTTCACCAAT+TGG - chr5.3:28945815-28945834 MS.gene045645:intron 35.0%
TACTCTGACAACAAAGAACA+AGG - chr5.3:28944951-28944970 MS.gene045645:CDS 35.0%
TAGCCAATATCAGAATGACA+TGG - chr5.3:28946309-28946328 MS.gene045645:CDS 35.0%
TATATGTGATGAAATCACGG+TGG + chr5.3:28945642-28945661 None:intergenic 35.0%
TCAGATCTAACAGAATCAAG+AGG - chr5.3:28945911-28945930 MS.gene045645:intron 35.0%
TGAAGAATTGTATCCAGAGA+GGG + chr5.3:28945810-28945829 None:intergenic 35.0%
TGGAACTTCTTTGTGTTCAT+TGG - chr5.3:28945970-28945989 MS.gene045645:CDS 35.0%
TTCACTTGAATTGCCACTAA+TGG - chr5.3:28946084-28946103 MS.gene045645:CDS 35.0%
! AGAACAAGGTAATGACTTGT+AGG - chr5.3:28944965-28944984 MS.gene045645:CDS 35.0%
! GAACAAGGTAATGACTTGTA+GGG - chr5.3:28944966-28944985 MS.gene045645:CDS 35.0%
! GATTCTGTTAGATCTGAGAT+TGG + chr5.3:28945908-28945927 None:intergenic 35.0%
! TTGTTCTTTGTTGTCAGAGT+AGG + chr5.3:28944952-28944971 None:intergenic 35.0%
!! CAAGTACATTTTTGGATTGG+CGG - chr5.3:28945536-28945555 MS.gene045645:intron 35.0%
!! CATAACATTGATGGTCCTTT+TGG - chr5.3:28945194-28945213 MS.gene045645:intron 35.0%
!! GAAGAGTATTATGAGAGTGA+TGG - chr5.3:28944755-28944774 MS.gene045645:CDS 35.0%
!! TCATGCTCTAGAAATGCAAA+TGG - chr5.3:28945423-28945442 MS.gene045645:intron 35.0%
!!! TTCTGTTTTTGCCAGAAACA+TGG - chr5.3:28945393-28945412 MS.gene045645:intron 35.0%
ACCATCTCGTATCAAAACAG+TGG - chr5.3:28945034-28945053 MS.gene045645:CDS 40.0%
AGATGGTACAAAGTAAGTCC+TGG + chr5.3:28944786-28944805 None:intergenic 40.0%
ATGCAAAACCAAGGCTCAAA+TGG - chr5.3:28944548-28944567 MS.gene045645:CDS 40.0%
CGCCAATCCAAAAATGTACT+TGG + chr5.3:28945538-28945557 None:intergenic 40.0%
GTGAAGAATTGTATCCAGAG+AGG + chr5.3:28945811-28945830 None:intergenic 40.0%
TATCCACTGATGCAAAACCA+AGG - chr5.3:28944539-28944558 MS.gene045645:CDS 40.0%
! AGATCTGAGATTGGAGAACT+TGG + chr5.3:28945899-28945918 None:intergenic 40.0%
! ATGAGAGTGATGGAAATTCC+AGG - chr5.3:28944765-28944784 MS.gene045645:CDS 40.0%
!! CAGAGTAGGTTTCTAGTTGT+TGG + chr5.3:28944938-28944957 None:intergenic 40.0%
!!! ACCACTGTTTTGATACGAGA+TGG + chr5.3:28945038-28945057 None:intergenic 40.0%
AATTGCCACTAATGGCCATG+CGG - chr5.3:28946092-28946111 MS.gene045645:CDS 45.0%
CAGCAGAGAAATCAGTCTTG+GGG - chr5.3:28945256-28945275 MS.gene045645:intron 45.0%
CCATCTCAAGAGCCATTTAC+AGG - chr5.3:28944800-28944819 MS.gene045645:CDS 45.0%
CCTGTAAATGGCTCTTGAGA+TGG + chr5.3:28944803-28944822 None:intergenic 45.0%
CGAAGCACAGATACAGACAT+TGG + chr5.3:28946142-28946161 None:intergenic 45.0%
CGGTGAGTTCAGTAGAATCA+TGG - chr5.3:28945556-28945575 MS.gene045645:intron 45.0%
GAGGAGAGGAAACATGACAA+TGG - chr5.3:28945950-28945969 MS.gene045645:CDS 45.0%
GCAGCAGAGAAATCAGTCTT+GGG - chr5.3:28945255-28945274 MS.gene045645:intron 45.0%
GCATTGTTCATCACAGCTCT+TGG + chr5.3:28945237-28945256 None:intergenic 45.0%
TCTGTGTACACTGCACCAAA+AGG + chr5.3:28945212-28945231 None:intergenic 45.0%
TGATTTCTCTTCTGACCGCA+TGG + chr5.3:28946110-28946129 None:intergenic 45.0%
TGCAGCAGAGAAATCAGTCT+TGG - chr5.3:28945254-28945273 MS.gene045645:intron 45.0%
! ATGGTGTGCTACCGTGATTT+TGG - chr5.3:28945575-28945594 MS.gene045645:intron 45.0%
CAGCTTCGAATCGAAGCTCA+AGG - chr5.3:28945746-28945765 MS.gene045645:intron 50.0%
TCAGGAGTCCATTTGAGCCT+TGG + chr5.3:28944559-28944578 None:intergenic 50.0%
! ATCGGTACTAATGAAGGCGC+AGG - chr5.3:28945781-28945800 MS.gene045645:intron 50.0%
!!! GAGCCTTGGTTTTGCATCAG+TGG + chr5.3:28944545-28944564 None:intergenic 50.0%
TCTGACCGCATGGCCATTAG+TGG + chr5.3:28946100-28946119 None:intergenic 55.0%
! GGTACTAATGAAGGCGCAGG+AGG - chr5.3:28945784-28945803 MS.gene045645:intron 55.0%
AAGGCGCAGGAGGCCCTCTC+TGG - chr5.3:28945794-28945813 MS.gene045645:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 28944511 28946393 28944511 ID=MS.gene045645
chr5.3 mRNA 28944511 28946393 28944511 ID=MS.gene045645.t1;Parent=MS.gene045645
chr5.3 exon 28946276 28946393 28946276 ID=MS.gene045645.t1.exon1;Parent=MS.gene045645.t1
chr5.3 CDS 28946276 28946393 28946276 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
chr5.3 exon 28946083 28946159 28946083 ID=MS.gene045645.t1.exon2;Parent=MS.gene045645.t1
chr5.3 CDS 28946083 28946159 28946083 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
chr5.3 exon 28945932 28945989 28945932 ID=MS.gene045645.t1.exon3;Parent=MS.gene045645.t1
chr5.3 CDS 28945932 28945989 28945932 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
chr5.3 exon 28945607 28945682 28945607 ID=MS.gene045645.t1.exon4;Parent=MS.gene045645.t1
chr5.3 CDS 28945607 28945682 28945607 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
chr5.3 exon 28945427 28945496 28945427 ID=MS.gene045645.t1.exon5;Parent=MS.gene045645.t1
chr5.3 CDS 28945427 28945496 28945427 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
chr5.3 exon 28944511 28945173 28944511 ID=MS.gene045645.t1.exon6;Parent=MS.gene045645.t1
chr5.3 CDS 28944511 28945173 28944511 ID=cds.MS.gene045645.t1;Parent=MS.gene045645.t1
Gene Sequence

>MS.gene045645

ATGCAGAATCAAAGTATGCATTTTGTTCTATCCACTGATGCAAAACCAAGGCTCAAATGGACTCCTGAACTTCACCAAAGATTCATTGATGCAATTAATCAACTTGGAGGTGCAGCTAGTAAGTACAAAACAAACTCAATAATGACCTTACTATTTTTTTTCATATTTTTATTACACTTTAGAGCATAAATGTTACTTGATGTATCATTCAAATTGTGTATGTTAATGTGGCAGAAGCAACACCGAAGAGTATTATGAGAGTGATGGAAATTCCAGGACTTACTTTGTACCATCTCAAGAGCCATTTACAGGTATTACCTTTTTTGTAATGATATTATAATTTTTTGGTTATTTTTTGTTGACTGAAGAAGTTGTGACTTTTCATGTTTTTTTACTGGTTGTAGAAATATAGACTTGGGAAAAGCCAACAACTAGAAACCTACTCTGACAACAAAGAACAAGGTAATGACTTGTAGGGAAACATATTTACTTCAGTAACTGTGTCGCACAGTTTTCAATCTCGACCATCTCGTATCAAAACAGTGGTCGAGAGTGTTTATAGACTAATTGATTTTGTTATTGTGTAATTTGAGCCGTCTGATTTCAAATTAACGGTTAAGATTGTTTACAACGTGTAAATGTGTGTGTGTCTGTTAATCCAAATAGTATGATCGATCATTTGGCATAACATTGATGGTCCTTTTGGTGCAGTGTACACAGAAACCAAGAGCTGTGATGAACAATGCAGCAGAGAAATCAGTCTTGGGGACCAGAATCAAATCACTGAGTAATAATTAGAGCTTTTTCAAATATTCGATTATGATTTTTTCGAAAAAAAAATTTTAGATTGAAATAGTATTACCTAGAGATTTTATTTTCAAATTCTGTTTTTGCCAGAAACATGGAGATATCTCATGCTCTAGAAATGCAAATGGAAGTAGAAAGGAAACTTAATGAACAAATTGAGGTAACATAAATAATGTGATATCATGATTAAAATTTGCTTCAATCATAATTCTAACTTCCAAGTACATTTTTGGATTGGCGGTGAGTTCAGTAGAATCATGGTGTGCTACCGTGATTTTGGTAAAAAAAAAATACACTTTAAGCTGCAACAAAATCAAGTTGCCACCGTGATTTCATCACATATACATTGTGTATCCAAATATGCACTAAATGTTGCATAAGGACGTGTTAATCAATTTGGTTGTTCATTTCAGGTGCAGAAACATTTGCAGCTTCGAATCGAAGCTCAAGGAAAATATTTACAATCGGTACTAATGAAGGCGCAGGAGGCCCTCTCTGGATACAATTCTTCACCAATTGGAATAAAACTGACAAAAGACGAACTGTCTCAATTGGTTACTATGATCAACAATGCATGTCCAAGTTCTCCAATCTCAGATCTAACAGAATCAAGAGGGTTAAGTTTAAACTATGAGGAGAGGAAACATGACAATGGAACTTCTTTGTGTTCATTGGAAAGTTCTTTGACATCTTCTGAAATCTCAGAGAGAAATGAAGAGAAACATTCATTAGAAGAAATAGGAGACTTCAAAAATTCTAGTGCTCTTTCACTTGAATTGCCACTAATGGCCATGCGGTCAGAAGAGAAATCAGTGTCGTGTCCAATGTCTGTATCTGTGCTTCGTAGCGATAATTCAAGTAATGAAGCTAATGTTAGAAAGAGAAATGAAGAAACTAAATTTGATGGTAGCTATGTTGAGAATTATTCATTAAGGAAAAGAGGTGGCAACAAGTTGAAGAAAGCTAAGTTGTCAGAAAAATTTGACTTGAATAGCCAATATCAGAATGACATGGACTCAACAAGTTCAAAAACGTTATTAGACTTAAATTGCAGTTTAAACTTTTATGAACCATGA
Protein sequence

>MS.gene045645.t1

MQNQSMHFVLSTDAKPRLKWTPELHQRFIDAINQLGGAAKATPKSIMRVMEIPGLTLYHLKSHLQKYRLGKSQQLETYSDNKEQVYTETKSCDEQCSREISLGDQNQITENMEISHALEMQMEVERKLNEQIEVQKHLQLRIEAQGKYLQSVLMKAQEALSGYNSSPIGIKLTKDELSQLVTMINNACPSSPISDLTESRGLSLNYEERKHDNGTSLCSLESSLTSSEISERNEEKHSLEEIGDFKNSSALSLELPLMAMRSEEKSVSCPMSVSVLRSDNSSNEANVRKRNEETKFDGSYVENYSLRKRGGNKLKKAKLSEKFDLNSQYQNDMDSTSSKTLLDLNCSLNFYEP