Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045868.t1 | PNY07851.1 | 35.2 | 213 | 136 | 2 | 13 | 223 | 12 | 224 | 3.30E-32 | 148.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045868.t1 | A0A2K3NXT8 | 35.2 | 213 | 136 | 2 | 13 | 223 | 12 | 224 | 2.4e-32 | 148.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene045868.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045868.t1 | MTR_4g123470 | 28.141 | 199 | 143 | 0 | 10 | 208 | 12 | 210 | 5.30e-28 | 106 |
| MS.gene045868.t1 | MTR_0014s0220 | 26.108 | 203 | 132 | 5 | 12 | 213 | 628 | 813 | 1.56e-15 | 75.5 |
| MS.gene045868.t1 | MTR_1g054495 | 22.798 | 193 | 148 | 1 | 17 | 209 | 16 | 207 | 2.37e-15 | 72.8 |
| MS.gene045868.t1 | MTR_4g085790 | 24.121 | 199 | 150 | 1 | 25 | 223 | 24 | 221 | 1.96e-14 | 70.1 |
| MS.gene045868.t1 | MTR_7g025150 | 24.103 | 195 | 147 | 1 | 29 | 223 | 28 | 221 | 2.52e-14 | 69.7 |
| MS.gene045868.t1 | MTR_1g090753 | 32.632 | 95 | 64 | 0 | 22 | 116 | 38 | 132 | 5.09e-14 | 67.8 |
| MS.gene045868.t1 | MTR_4g053550 | 45.714 | 70 | 38 | 0 | 27 | 96 | 49 | 118 | 6.32e-14 | 66.6 |
| MS.gene045868.t1 | MTR_5g074790 | 31.731 | 104 | 71 | 0 | 14 | 117 | 160 | 263 | 1.57e-12 | 65.5 |
| MS.gene045868.t1 | MTR_1g054485 | 23.353 | 167 | 128 | 0 | 22 | 188 | 21 | 187 | 3.30e-12 | 63.2 |
| MS.gene045868.t1 | MTR_7g039210 | 20.548 | 219 | 143 | 4 | 12 | 199 | 10 | 228 | 7.30e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAATGTGATGTAATAATA+AGG | + | 57133:38765-38784 | MS.gene045868:CDS | 20.0% |
| !! | CAAATGTGATGTAATAATAA+GGG | + | 57133:38766-38785 | MS.gene045868:CDS | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | + | 57133:38827-38846 | MS.gene045868:CDS | 20.0% |
| ! | AAGTACATCAGATATAATGA+AGG | + | 57133:38892-38911 | MS.gene045868:CDS | 25.0% |
| ! | AATAAGGGATAGAAAAAAGA+AGG | + | 57133:38781-38800 | MS.gene045868:CDS | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | + | 57133:38433-38452 | MS.gene045868:CDS | 25.0% |
| ! | AGTGCTCAACATAAAATAAT+AGG | - | 57133:38185-38204 | None:intergenic | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | + | 57133:38434-38453 | MS.gene045868:CDS | 25.0% |
| ! | TACTCTGCTATTAATATGTA+TGG | + | 57133:38725-38744 | MS.gene045868:CDS | 25.0% |
| ! | TATTACATCACATTTGTACT+CGG | - | 57133:38762-38781 | None:intergenic | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | 57133:38865-38884 | MS.gene045868:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | 57133:38794-38813 | MS.gene045868:CDS | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | + | 57133:38562-38581 | MS.gene045868:CDS | 30.0% | |
| ATAAAATGCTCAACAAAGGA+AGG | - | 57133:38272-38291 | None:intergenic | 30.0% | |
| ATATCTCATTTACAGACGTA+TGG | + | 57133:38236-38255 | MS.gene045868:intron | 30.0% | |
| ATCATTTGAGAATGAGTTGT+TGG | - | 57133:38612-38631 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | + | 57133:38734-38753 | MS.gene045868:CDS | 30.0% | |
| CAGATATAATGAAGGTTTCT+AGG | + | 57133:38900-38919 | MS.gene045868:CDS | 30.0% | |
| GAACTTAACTTTGTGTCTAT+TGG | - | 57133:38336-38355 | None:intergenic | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | - | 57133:38556-38575 | None:intergenic | 30.0% | |
| TAAATTCTCTTCCAAGTTTG+TGG | - | 57133:38295-38314 | None:intergenic | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | + | 57133:38149-38168 | MS.gene045868:CDS | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | + | 57133:38435-38454 | MS.gene045868:CDS | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | + | 57133:38395-38414 | MS.gene045868:CDS | 30.0% |
| ATCTACTTCAAGGACTCATA+GGG | + | 57133:38110-38129 | MS.gene045868:CDS | 35.0% | |
| ATGTTAACTCATTGCCATCA+TGG | + | 57133:38498-38517 | MS.gene045868:CDS | 35.0% | |
| GTGGATAAAATGCTCAACAA+AGG | - | 57133:38276-38295 | None:intergenic | 35.0% | |
| TTCCAATTCACTGCATTTCA+TGG | + | 57133:38467-38486 | MS.gene045868:CDS | 35.0% | |
| ! | GAGCATTTTATCCACAAACT+TGG | + | 57133:38281-38300 | MS.gene045868:CDS | 35.0% |
| ! | TGTAAAGACAATGGATTCCA+TGG | + | 57133:38836-38855 | MS.gene045868:CDS | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | + | 57133:38399-38418 | MS.gene045868:CDS | 35.0% |
| CACTTACAAGCAAGGAATTG+AGG | + | 57133:38523-38542 | MS.gene045868:CDS | 40.0% | |
| GATCTACTTCAAGGACTCAT+AGG | + | 57133:38109-38128 | MS.gene045868:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | + | 57133:38701-38720 | MS.gene045868:CDS | 40.0% | |
| GTCCATGAAATGCAGTGAAT+TGG | - | 57133:38472-38491 | None:intergenic | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | + | 57133:38580-38599 | MS.gene045868:CDS | 40.0% | |
| TCAAGGACTCATAGGGATAA+TGG | + | 57133:38117-38136 | MS.gene045868:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | + | 57133:38385-38404 | MS.gene045868:CDS | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | - | 57133:38578-38597 | None:intergenic | 40.0% | |
| TGCAGCCATATAAGGAGTTA+AGG | - | 57133:38570-38589 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | + | 57133:38569-38588 | MS.gene045868:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | + | 57133:38839-38858 | MS.gene045868:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | + | 57133:38840-38859 | MS.gene045868:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | + | 57133:38680-38699 | MS.gene045868:intron | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | 57133:38800-38819 | MS.gene045868:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | - | 57133:38856-38875 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | + | 57133:38801-38820 | MS.gene045868:CDS | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | + | 57133:38384-38403 | MS.gene045868:CDS | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | + | 57133:38368-38387 | MS.gene045868:CDS | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | 57133:38515-38534 | MS.gene045868:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | + | 57133:38367-38386 | MS.gene045868:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | 57133:38515-38534 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | + | 57133:38841-38860 | MS.gene045868:CDS | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | + | 57133:38372-38391 | MS.gene045868:CDS | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | - | 57133:38377-38396 | None:intergenic | 50.0% | |
| CAACAAAGATTGGCAGTGGG+TGG | + | 57133:38804-38823 | MS.gene045868:CDS | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | 57133:38752-38771 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCTACTTCA+AGG | + | 57133:38100-38119 | MS.gene045868:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57133 | gene | 38096 | 38928 | 38096 | ID=MS.gene045868 |
| 57133 | mRNA | 38096 | 38928 | 38096 | ID=MS.gene045868.t1;Parent=MS.gene045868 |
| 57133 | exon | 38096 | 38170 | 38096 | ID=MS.gene045868.t1.exon1;Parent=MS.gene045868.t1 |
| 57133 | CDS | 38096 | 38170 | 38096 | ID=cds.MS.gene045868.t1;Parent=MS.gene045868.t1 |
| 57133 | exon | 38251 | 38601 | 38251 | ID=MS.gene045868.t1.exon2;Parent=MS.gene045868.t1 |
| 57133 | CDS | 38251 | 38601 | 38251 | ID=cds.MS.gene045868.t1;Parent=MS.gene045868.t1 |
| 57133 | exon | 38683 | 38928 | 38683 | ID=MS.gene045868.t1.exon3;Parent=MS.gene045868.t1 |
| 57133 | CDS | 38683 | 38928 | 38683 | ID=cds.MS.gene045868.t1;Parent=MS.gene045868.t1 |
| Gene Sequence |
| Protein sequence |
