Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046043.t1 | XP_013455584.1 | 97.9 | 96 | 2 | 0 | 13 | 108 | 59 | 154 | 4.60E-48 | 200.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046043.t1 | O05517 | 35.7 | 70 | 45 | 0 | 26 | 95 | 69 | 138 | 3.7e-06 | 52.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046043.t1 | G7ZX36 | 97.9 | 96 | 2 | 0 | 13 | 108 | 59 | 154 | 3.3e-48 | 200.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046043.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046043.t1 | MTR_4g046047 | 97.917 | 96 | 2 | 0 | 13 | 108 | 59 | 154 | 2.89e-69 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046043.t1 | AT1G03650 | 76.842 | 95 | 22 | 0 | 14 | 108 | 63 | 157 | 2.15e-51 | 159 |
Find 26 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTATGTCATGTATTCT+TGG | 0.193464 | 4.4:-57345586 | MS.gene046043:CDS |
| CATGTTGACTGTTTGGTTGA+AGG | 0.355783 | 4.4:-57344462 | MS.gene046043:CDS |
| TGGCTTTCATGTTGACTGTT+TGG | 0.383570 | 4.4:-57344469 | MS.gene046043:CDS |
| TGGACTTCGATTCAAATTGA+AGG | 0.474763 | 4.4:-57344401 | None:intergenic |
| GGAGCTGGAACCTGCGAGCT+TGG | 0.496063 | 4.4:+57345538 | None:intergenic |
| AGGGCATGGAGAGGTTCTAC+TGG | 0.510423 | 4.4:-57344589 | MS.gene046043:CDS |
| AAATGCTTATAGAATGTACT+TGG | 0.517363 | 4.4:-57344421 | MS.gene046043:CDS |
| CAATGGAGGAGGCAAGGGCA+TGG | 0.517572 | 4.4:-57344603 | MS.gene046043:CDS |
| TCTTGTACAGATTCAGCGCA+GGG | 0.523854 | 4.4:+57344496 | None:intergenic |
| AAGGAGCAATGGAGGAGGCA+AGG | 0.528602 | 4.4:-57344609 | MS.gene046043:CDS |
| GCAGAACAAGAAAAGTTTCG+CGG | 0.541873 | 4.4:-57344548 | MS.gene046043:CDS |
| TGGAGAGGTTCTACTGGAAG+CGG | 0.545320 | 4.4:-57344583 | MS.gene046043:CDS |
| GCTTCTATCACCAAGCTCGC+AGG | 0.549548 | 4.4:-57345548 | MS.gene046043:intron |
| TTCTTGTACAGATTCAGCGC+AGG | 0.552919 | 4.4:+57344495 | None:intergenic |
| TGCTTCACGTCGATCCGTTG+AGG | 0.562820 | 4.4:-57344521 | MS.gene046043:CDS |
| ATAGAAGCATACAATGAAGA+AGG | 0.562971 | 4.4:+57345563 | None:intergenic |
| AGGAGCAATGGAGGAGGCAA+GGG | 0.571699 | 4.4:-57344608 | MS.gene046043:CDS |
| CTTCTGTAGTGAAGGAGCAA+TGG | 0.596774 | 4.4:-57344620 | MS.gene046043:intron |
| CTGAATCTGTACAAGAAGCA+TGG | 0.599242 | 4.4:-57344489 | MS.gene046043:CDS |
| CTGTAGTGAAGGAGCAATGG+AGG | 0.602271 | 4.4:-57344617 | MS.gene046043:intron |
| TAGTGAAGGAGCAATGGAGG+AGG | 0.603189 | 4.4:-57344614 | MS.gene046043:CDS |
| CGCACGAGTGATGGCAACTG+TGG | 0.609912 | 4.4:-57345771 | None:intergenic |
| TTCAGCGCAGGGGTCCTCAA+CGG | 0.614591 | 4.4:+57344507 | None:intergenic |
| TTAGAACTTGACAGAAACAG+TGG | 0.638839 | 4.4:-57345743 | MS.gene046043:intron |
| GAGGAGGCAAGGGCATGGAG+AGG | 0.640974 | 4.4:-57344598 | MS.gene046043:CDS |
| CTTGTACAGATTCAGCGCAG+GGG | 0.671497 | 4.4:+57344497 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATTATTCAATTTAT+AGG | - | chr4.4:57345017-57345036 | MS.gene046043:intron | 10.0% |
| !! | AAGAAATTATTCAATTTATA+GGG | - | chr4.4:57345018-57345037 | MS.gene046043:intron | 10.0% |
| !! | AATTAACTATCTTTAATATA+AGG | - | chr4.4:57345406-57345425 | MS.gene046043:intron | 10.0% |
| !! | ATTAACTATCTTTAATATAA+GGG | - | chr4.4:57345407-57345426 | MS.gene046043:intron | 10.0% |
| !! | AGAAATTATTCAATTTATAG+GGG | - | chr4.4:57345019-57345038 | MS.gene046043:intron | 15.0% |
| ! | AAAATTTGCAACCCATTTAT+AGG | - | chr4.4:57344954-57344973 | MS.gene046043:intron | 25.0% |
| ! | AAATGCTTATAGAATGTACT+TGG | - | chr4.4:57345744-57345763 | MS.gene046043:CDS | 25.0% |
| ! | ACAATATGGGTATGTAATAT+GGG | - | chr4.4:57345511-57345530 | MS.gene046043:intron | 25.0% |
| ! | ATACAAGCATTTCATTAGTT+GGG | + | chr4.4:57345464-57345483 | None:intergenic | 25.0% |
| ! | CTAAACTATTGAAAGATAGT+AGG | + | chr4.4:57345202-57345221 | None:intergenic | 25.0% |
| ! | TTATGCAAACTCCTATAAAT+GGG | + | chr4.4:57344968-57344987 | None:intergenic | 25.0% |
| !! | AAAATTGTCTAAGTGTGTTT+TGG | - | chr4.4:57344692-57344711 | MS.gene046043:intron | 25.0% |
| !!! | ACCAGGTTTTATAATAATTC+TGG | + | chr4.4:57344929-57344948 | None:intergenic | 25.0% |
| !!! | CAATTTTTCAAACAGCTTAT+AGG | + | chr4.4:57344679-57344698 | None:intergenic | 25.0% |
| !!! | GTTGCAAATTTTTTTTTACC+AGG | + | chr4.4:57344946-57344965 | None:intergenic | 25.0% |
| !!! | TTTGAGTTCATTTTCAAAGA+AGG | + | chr4.4:57344513-57344532 | None:intergenic | 25.0% |
| AAATTGCAAAAACATTCAGC+CGG | - | chr4.4:57345075-57345094 | MS.gene046043:intron | 30.0% | |
| CACAATATGGGTATGTAATA+TGG | - | chr4.4:57345510-57345529 | MS.gene046043:intron | 30.0% | |
| CATACAAGCATTTCATTAGT+TGG | + | chr4.4:57345465-57345484 | None:intergenic | 30.0% | |
| CTTATGCAAACTCCTATAAA+TGG | + | chr4.4:57344969-57344988 | None:intergenic | 30.0% | |
| GCCAGAATTATTATAAAACC+TGG | - | chr4.4:57344925-57344944 | MS.gene046043:intron | 30.0% | |
| TAATACATCAACTTAGTCGT+TGG | - | chr4.4:57345165-57345184 | MS.gene046043:intron | 30.0% | |
| ! | ATAGAAGCATACAATGAAGA+AGG | + | chr4.4:57344605-57344624 | None:intergenic | 30.0% |
| ! | GTATGTAATATGGGATGTTT+TGG | - | chr4.4:57345520-57345539 | MS.gene046043:intron | 30.0% |
| ! | TTGTTGGTTACTCACAATAT+GGG | - | chr4.4:57345498-57345517 | MS.gene046043:intron | 30.0% |
| ! | TTTGTTGGTTACTCACAATA+TGG | - | chr4.4:57345497-57345516 | MS.gene046043:intron | 30.0% |
| !! | ACGACTAAGTTGATGTATTA+AGG | + | chr4.4:57345165-57345184 | None:intergenic | 30.0% |
| !! | ACTAAGTTGATGTATTAAGG+GGG | + | chr4.4:57345162-57345181 | None:intergenic | 30.0% |
| !! | CGACTAAGTTGATGTATTAA+GGG | + | chr4.4:57345164-57345183 | None:intergenic | 30.0% |
| !! | GACTAAGTTGATGTATTAAG+GGG | + | chr4.4:57345163-57345182 | None:intergenic | 30.0% |
| AGTGGAAGAGATAGTGAAAT+TGG | - | chr4.4:57344451-57344470 | MS.gene046043:CDS | 35.0% | |
| CAGGTTATGTCATGTATTCT+TGG | - | chr4.4:57344579-57344598 | MS.gene046043:CDS | 35.0% | |
| CATTGTCGATATAGTTAGGT+TGG | + | chr4.4:57345373-57345392 | None:intergenic | 35.0% | |
| CTATATCGACAATGTCAGTT+AGG | - | chr4.4:57345379-57345398 | MS.gene046043:intron | 35.0% | |
| CTGACATTGTCGATATAGTT+AGG | + | chr4.4:57345377-57345396 | None:intergenic | 35.0% | |
| GAACTCAAAAAGAAGAACAG+TGG | - | chr4.4:57344524-57344543 | MS.gene046043:CDS | 35.0% | |
| GTGAGTAACCAACAAATCAA+AGG | + | chr4.4:57345493-57345512 | None:intergenic | 35.0% | |
| TGATTTGAGCAAAAGGCTAA+AGG | + | chr4.4:57345129-57345148 | None:intergenic | 35.0% | |
| TTAGAACTTGACAGAAACAG+TGG | - | chr4.4:57344422-57344441 | MS.gene046043:CDS | 35.0% | |
| TTGTGTGTGTGAATTTCATG+TGG | + | chr4.4:57345316-57345335 | None:intergenic | 35.0% | |
| ! | CTTGAGAGCATAAAAAAGAG+TGG | + | chr4.4:57344651-57344670 | None:intergenic | 35.0% |
| ! | GAACTTTTGTAAGTGTGACT+CGG | - | chr4.4:57344834-57344853 | MS.gene046043:intron | 35.0% |
| !! | ACTCAACTGATTTGAGCAAA+AGG | + | chr4.4:57345136-57345155 | None:intergenic | 35.0% |
| !! | ATGCGAATCCTTTGATTTGT+TGG | - | chr4.4:57345482-57345501 | MS.gene046043:intron | 35.0% |
| !!! | TTTTGGTTCTTCTGTAGTGA+AGG | - | chr4.4:57345537-57345556 | MS.gene046043:intron | 35.0% |
| AAGCTTGGATATTGGTATGC+AGG | - | chr4.4:57344862-57344881 | MS.gene046043:intron | 40.0% | |
| AATTCACACACACAACTGCA+AGG | - | chr4.4:57345321-57345340 | MS.gene046043:intron | 40.0% | |
| ACATGACATAACCTGCAAGT+TGG | + | chr4.4:57344574-57344593 | None:intergenic | 40.0% | |
| AGCATAAAAAAGAGTGGAGC+TGG | + | chr4.4:57344645-57344664 | None:intergenic | 40.0% | |
| AGCTTGGATATTGGTATGCA+GGG | - | chr4.4:57344863-57344882 | MS.gene046043:intron | 40.0% | |
| CAGAAACAGTGGTAAAGTAG+TGG | - | chr4.4:57344433-57344452 | MS.gene046043:CDS | 40.0% | |
| CATGACATAACCTGCAAGTT+GGG | + | chr4.4:57344573-57344592 | None:intergenic | 40.0% | |
| CTGAATCTGTACAAGAAGCA+TGG | - | chr4.4:57345676-57345695 | MS.gene046043:intron | 40.0% | |
| GCAGAACAAGAAAAGTTTCG+CGG | - | chr4.4:57345617-57345636 | MS.gene046043:intron | 40.0% | |
| TGAGCAAAAGGCTAAAGGTA+GGG | + | chr4.4:57345124-57345143 | None:intergenic | 40.0% | |
| TTGAGCAAAAGGCTAAAGGT+AGG | + | chr4.4:57345125-57345144 | None:intergenic | 40.0% | |
| ! | CATGTTGACTGTTTGGTTGA+AGG | - | chr4.4:57345703-57345722 | MS.gene046043:intron | 40.0% |
| ! | TGGCTTTCATGTTGACTGTT+TGG | - | chr4.4:57345696-57345715 | MS.gene046043:intron | 40.0% |
| !!! | TTTTGTAAGTGTGACTCGGT+TGG | - | chr4.4:57344838-57344857 | MS.gene046043:intron | 40.0% |
| ACACACAACTGCAAGGTCTT+GGG | - | chr4.4:57345328-57345347 | MS.gene046043:intron | 45.0% | |
| CTTCTGTAGTGAAGGAGCAA+TGG | - | chr4.4:57345545-57345564 | MS.gene046043:intron | 45.0% | |
| GAAGCGAGTGATTCGTGTTT+GGG | + | chr4.4:57344491-57344510 | None:intergenic | 45.0% | |
| GAGCAAAAGGCTAAAGGTAG+GGG | + | chr4.4:57345123-57345142 | None:intergenic | 45.0% | |
| GATATTGGTATGCAGGGTTC+GGG | - | chr4.4:57344869-57344888 | MS.gene046043:intron | 45.0% | |
| GGATATTGGTATGCAGGGTT+CGG | - | chr4.4:57344868-57344887 | MS.gene046043:intron | 45.0% | |
| TCTTGTACAGATTCAGCGCA+GGG | + | chr4.4:57345672-57345691 | None:intergenic | 45.0% | |
| TTCTTGTACAGATTCAGCGC+AGG | + | chr4.4:57345673-57345692 | None:intergenic | 45.0% | |
| !! | CGGTTGGTAAGCTTGGATAT+TGG | - | chr4.4:57344854-57344873 | MS.gene046043:intron | 45.0% |
| AAAAACATTCAGCCGGAGCC+CGG | - | chr4.4:57345082-57345101 | MS.gene046043:intron | 50.0% | |
| CAAAAGGCTAAAGGTAGGGG+CGG | + | chr4.4:57345120-57345139 | None:intergenic | 50.0% | |
| CACACACAACTGCAAGGTCT+TGG | - | chr4.4:57345327-57345346 | MS.gene046043:intron | 50.0% | |
| CTGTAGTGAAGGAGCAATGG+AGG | - | chr4.4:57345548-57345567 | MS.gene046043:intron | 50.0% | |
| CTTGTACAGATTCAGCGCAG+GGG | + | chr4.4:57345671-57345690 | None:intergenic | 50.0% | |
| GGAAGCGAGTGATTCGTGTT+TGG | + | chr4.4:57344492-57344511 | None:intergenic | 50.0% | |
| GGTTCGAACCATTTAGCCAG+TGG | - | chr4.4:57344898-57344917 | MS.gene046043:intron | 50.0% | |
| TAAAGGTAGGGGCGGTTCTA+AGG | + | chr4.4:57345112-57345131 | None:intergenic | 50.0% | |
| TAGTGAAGGAGCAATGGAGG+AGG | - | chr4.4:57345551-57345570 | MS.gene046043:CDS | 50.0% | |
| TCGAACCATTTAGCCAGTGG+CGG | - | chr4.4:57344901-57344920 | MS.gene046043:intron | 50.0% | |
| TGGAGAGGTTCTACTGGAAG+CGG | - | chr4.4:57345582-57345601 | MS.gene046043:CDS | 50.0% | |
| !! | AATAATTCTGGCTCCGCCAC+TGG | + | chr4.4:57344917-57344936 | None:intergenic | 50.0% |
| !! | TGTGACTCGGTTGGTAAGCT+TGG | - | chr4.4:57344847-57344866 | MS.gene046043:intron | 50.0% |
| AAAACATTCAGCCGGAGCCC+GGG | - | chr4.4:57345083-57345102 | MS.gene046043:intron | 55.0% | |
| AAGGAGCAATGGAGGAGGCA+AGG | - | chr4.4:57345556-57345575 | MS.gene046043:CDS | 55.0% | |
| AGGAGCAATGGAGGAGGCAA+GGG | - | chr4.4:57345557-57345576 | MS.gene046043:CDS | 55.0% | |
| AGGGCATGGAGAGGTTCTAC+TGG | - | chr4.4:57345576-57345595 | MS.gene046043:CDS | 55.0% | |
| CACGTTGATGCCCAACTTGC+AGG | - | chr4.4:57344560-57344579 | MS.gene046043:CDS | 55.0% | |
| GCTTCTATCACCAAGCTCGC+AGG | - | chr4.4:57344617-57344636 | MS.gene046043:intron | 55.0% | |
| GGTATGCAGGGTTCGGGATT+CGG | - | chr4.4:57344875-57344894 | MS.gene046043:intron | 55.0% | |
| GTATGCAGGGTTCGGGATTC+GGG | - | chr4.4:57344876-57344895 | MS.gene046043:intron | 55.0% | |
| TATGCAGGGTTCGGGATTCG+GGG | - | chr4.4:57344877-57344896 | MS.gene046043:intron | 55.0% | |
| TGCTTCACGTCGATCCGTTG+AGG | - | chr4.4:57345644-57345663 | MS.gene046043:intron | 55.0% | |
| AAACATTCAGCCGGAGCCCG+GGG | - | chr4.4:57345084-57345103 | MS.gene046043:intron | 60.0% | |
| CAATGGAGGAGGCAAGGGCA+TGG | - | chr4.4:57345562-57345581 | MS.gene046043:CDS | 60.0% | |
| TTCAGCGCAGGGGTCCTCAA+CGG | + | chr4.4:57345661-57345680 | None:intergenic | 60.0% | |
| ! | TGGCTCCGCCACTGGCTAAA+TGG | + | chr4.4:57344909-57344928 | None:intergenic | 60.0% |
| AACATTCAGCCGGAGCCCGG+GGG | - | chr4.4:57345085-57345104 | MS.gene046043:intron | 65.0% | |
| GGAGCTGGAACCTGCGAGCT+TGG | + | chr4.4:57344630-57344649 | None:intergenic | 65.0% | |
| ! | GAGGAGGCAAGGGCATGGAG+AGG | - | chr4.4:57345567-57345586 | MS.gene046043:CDS | 65.0% |
| ACATTCAGCCGGAGCCCGGG+GGG | - | chr4.4:57345086-57345105 | MS.gene046043:intron | 70.0% | |
| !! | TTCTAAGGCCCCCCGGGCTC+CGG | + | chr4.4:57345097-57345116 | None:intergenic | 70.0% |
| !! | GGGCGGTTCTAAGGCCCCCC+GGG | + | chr4.4:57345103-57345122 | None:intergenic | 75.0% |
| !! | GGGGCGGTTCTAAGGCCCCC+CGG | + | chr4.4:57345104-57345123 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 57344404 | 57345783 | 57344404 | ID=MS.gene046043 |
| chr4.4 | mRNA | 57344404 | 57345783 | 57344404 | ID=MS.gene046043.t1;Parent=MS.gene046043 |
| chr4.4 | exon | 57345744 | 57345783 | 57345744 | ID=MS.gene046043.t1.exon1;Parent=MS.gene046043.t1 |
| chr4.4 | CDS | 57345744 | 57345783 | 57345744 | ID=cds.MS.gene046043.t1;Parent=MS.gene046043.t1 |
| chr4.4 | exon | 57345549 | 57345605 | 57345549 | ID=MS.gene046043.t1.exon2;Parent=MS.gene046043.t1 |
| chr4.4 | CDS | 57345549 | 57345605 | 57345549 | ID=cds.MS.gene046043.t1;Parent=MS.gene046043.t1 |
| chr4.4 | exon | 57344404 | 57344633 | 57344404 | ID=MS.gene046043.t1.exon3;Parent=MS.gene046043.t1 |
| chr4.4 | CDS | 57344404 | 57344633 | 57344404 | ID=cds.MS.gene046043.t1;Parent=MS.gene046043.t1 |
| Gene Sequence |
| Protein sequence |
