Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046124.t1 | XP_003592777.1 | 93.4 | 377 | 25 | 0 | 5 | 381 | 75 | 451 | 2.20E-201 | 711.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046124.t1 | A9X1D0 | 26.3 | 358 | 221 | 15 | 28 | 369 | 148 | 478 | 8.5e-13 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046124.t1 | G7IF12 | 93.4 | 377 | 25 | 0 | 5 | 381 | 75 | 451 | 1.6e-201 | 711.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046124.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046124.t1 | MTR_1g115980 | 93.369 | 377 | 25 | 0 | 5 | 381 | 75 | 451 | 0.0 | 711 |
| MS.gene046124.t1 | MTR_1g115980 | 91.512 | 377 | 23 | 1 | 5 | 381 | 75 | 442 | 0.0 | 689 |
| MS.gene046124.t1 | MTR_1g115980 | 93.313 | 329 | 22 | 0 | 5 | 333 | 75 | 403 | 0.0 | 642 |
| MS.gene046124.t1 | MTR_1g115980 | 91.185 | 329 | 20 | 1 | 5 | 333 | 75 | 394 | 0.0 | 619 |
| MS.gene046124.t1 | MTR_1g115980 | 92.308 | 286 | 22 | 0 | 5 | 290 | 75 | 360 | 0.0 | 556 |
| MS.gene046124.t1 | MTR_1g115980 | 89.860 | 286 | 20 | 1 | 5 | 290 | 75 | 351 | 0.0 | 535 |
| MS.gene046124.t1 | MTR_3g082690 | 26.596 | 376 | 236 | 14 | 13 | 369 | 113 | 467 | 1.88e-17 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046124.t1 | AT3G55080 | 55.703 | 377 | 164 | 2 | 5 | 380 | 84 | 458 | 8.98e-148 | 426 |
| MS.gene046124.t1 | AT3G55080 | 56.065 | 371 | 160 | 2 | 11 | 380 | 6 | 374 | 1.99e-147 | 422 |
| MS.gene046124.t1 | AT3G55080 | 56.065 | 371 | 160 | 2 | 11 | 380 | 6 | 374 | 1.99e-147 | 422 |
| MS.gene046124.t1 | AT3G55080 | 56.977 | 344 | 145 | 2 | 38 | 380 | 2 | 343 | 2.55e-137 | 395 |
| MS.gene046124.t1 | AT3G55080 | 56.160 | 349 | 145 | 3 | 38 | 380 | 2 | 348 | 9.42e-135 | 389 |
| MS.gene046124.t1 | AT3G07670 | 29.952 | 207 | 132 | 8 | 38 | 234 | 12 | 215 | 9.28e-17 | 81.3 |
| MS.gene046124.t1 | AT3G07670 | 29.952 | 207 | 132 | 8 | 38 | 234 | 150 | 353 | 9.32e-17 | 82.0 |
| MS.gene046124.t1 | AT1G14030 | 28.326 | 233 | 121 | 11 | 24 | 232 | 117 | 327 | 2.58e-13 | 71.6 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATGCTCCGCAATTAAT+TGG | 0.158521 | 1.1:-81592593 | None:intergenic |
| CTGATAAAAGGTTTGATTTC+AGG | 0.171835 | 1.1:-81581490 | None:intergenic |
| CTCCGAGCCCTTTGCATCTT+TGG | 0.188607 | 1.1:-81591576 | None:intergenic |
| CCAACATGTTCACTGATAAA+AGG | 0.220029 | 1.1:-81581502 | None:intergenic |
| TCTTTCTTGTACCACTCTTC+AGG | 0.243444 | 1.1:+81592308 | MS.gene046124:CDS |
| CCGTCAAAGTTCAGTTTATC+AGG | 0.269055 | 1.1:+81581901 | MS.gene046124:CDS |
| TCCTCTGCGTCACACAAAAT+TGG | 0.272658 | 1.1:+81591523 | MS.gene046124:CDS |
| TTTCAGGTGCTTATAAGATA+TGG | 0.280416 | 1.1:+81591255 | MS.gene046124:intron |
| GCTGGCTAGGCGCCCTTTAC+AGG | 0.304269 | 1.1:+81592521 | MS.gene046124:CDS |
| TATTATGCATCTCGGCTTGC+GGG | 0.336854 | 1.1:-81581703 | None:intergenic |
| GGAGCATAATGCAGCTATTA+TGG | 0.347092 | 1.1:+81592605 | MS.gene046124:CDS |
| AATCACTTCGTGCACTTGCT+CGG | 0.349523 | 1.1:+81592283 | MS.gene046124:CDS |
| CCTGATAAACTGAACTTTGA+CGG | 0.359051 | 1.1:-81581901 | None:intergenic |
| TCTGATCGTGATTATGTTCC+TGG | 0.368828 | 1.1:+81585193 | MS.gene046124:CDS |
| CGAGCCATTCGTCTTCTAAC+AGG | 0.372330 | 1.1:-81592908 | None:intergenic |
| CTTTGTTCATATCCTGTAAA+GGG | 0.388424 | 1.1:-81592533 | None:intergenic |
| CCCTTTGCATCTTTGGTTGG+AGG | 0.393470 | 1.1:-81591569 | None:intergenic |
| GTGAAGTCTGCTGGAGGAAA+AGG | 0.398194 | 1.1:+81592246 | MS.gene046124:CDS |
| CTTTCTTGTACCACTCTTCA+GGG | 0.401046 | 1.1:+81592309 | MS.gene046124:CDS |
| GAGCCCTTTGCATCTTTGGT+TGG | 0.427651 | 1.1:-81591572 | None:intergenic |
| ATCACTTCGTGCACTTGCTC+GGG | 0.429403 | 1.1:+81592284 | MS.gene046124:CDS |
| ACCTGATCATAAATGTTGTA+TGG | 0.431705 | 1.1:-81591324 | None:intergenic |
| TCCATACAACATTTATGATC+AGG | 0.435626 | 1.1:+81591323 | MS.gene046124:CDS |
| ATTTCAGGAGGGAGATTATC+CGG | 0.436105 | 1.1:-81581475 | None:intergenic |
| AATGCCAGAGCTTAACCATT+TGG | 0.439954 | 1.1:+81592470 | MS.gene046124:intron |
| TGATTATGTTCCTGGTGAAC+AGG | 0.442198 | 1.1:+81585201 | MS.gene046124:CDS |
| CATAGGGAGGTGAAGTCTGC+TGG | 0.442386 | 1.1:+81592237 | MS.gene046124:intron |
| TCTTTGTTCATATCCTGTAA+AGG | 0.450034 | 1.1:-81592534 | None:intergenic |
| TTCAAATGCTACATTGATGC+TGG | 0.452747 | 1.1:+81591284 | MS.gene046124:CDS |
| CCAAAAGTCTCAAATTGAAA+AGG | 0.459340 | 1.1:+81581934 | MS.gene046124:CDS |
| GCTACATTGATGCTGGACTT+TGG | 0.464448 | 1.1:+81591291 | MS.gene046124:CDS |
| CAAATCTGCTTCTACCTGGC+TGG | 0.469867 | 1.1:+81592961 | MS.gene046124:CDS |
| AAACTGATGGTAGGCTGGCT+AGG | 0.476607 | 1.1:+81592508 | MS.gene046124:CDS |
| CAGATTTCTTGAATCATGAC+GGG | 0.480634 | 1.1:+81584595 | MS.gene046124:CDS |
| GCTTCTAAATCAATACAAAC+CGG | 0.487852 | 1.1:+81581330 | MS.gene046124:CDS |
| AACCAAAGATGCAAAGGGCT+CGG | 0.490231 | 1.1:+81591574 | MS.gene046124:CDS |
| GTGCAGCCTCCGTGACCAAA+TGG | 0.495854 | 1.1:-81592485 | None:intergenic |
| AATCCACAAGAATTTGGGCC+AGG | 0.496147 | 1.1:+81581555 | MS.gene046124:CDS |
| CATACTTAGGTATATCGTAC+TGG | 0.499200 | 1.1:-81591499 | None:intergenic |
| GACCAGTTGGCTCTAGAGCA+TGG | 0.502799 | 1.1:+81584463 | MS.gene046124:intron |
| AAGCACAAAGGGTTTATCTC+TGG | 0.505327 | 1.1:+81584488 | MS.gene046124:CDS |
| TGCACAAACTGATGGTAGGC+TGG | 0.508432 | 1.1:+81592503 | MS.gene046124:CDS |
| ATTCTTGTGGATTAAAAGAA+CGG | 0.515742 | 1.1:-81581545 | None:intergenic |
| ACCAGTTGGCTCTAGAGCAT+GGG | 0.517262 | 1.1:+81584464 | MS.gene046124:intron |
| GCAAGCCGAGATGCATAATA+CGG | 0.530146 | 1.1:+81581706 | MS.gene046124:CDS |
| GTATTATGCATCTCGGCTTG+CGG | 0.530651 | 1.1:-81581704 | None:intergenic |
| AGAGCATGGGGAAGCACAAA+GGG | 0.532315 | 1.1:+81584477 | MS.gene046124:CDS |
| TTCTCAAATCTGCTTCTACC+TGG | 0.536097 | 1.1:+81592957 | MS.gene046124:CDS |
| TGAAGTCTGCTGGAGGAAAA+GGG | 0.538297 | 1.1:+81592247 | MS.gene046124:CDS |
| GCCTCCAACCAAAGATGCAA+AGG | 0.545403 | 1.1:+81591568 | MS.gene046124:CDS |
| ATAAAAGGTTTGATTTCAGG+AGG | 0.546686 | 1.1:-81581487 | None:intergenic |
| ACTTGCCGTATTATGCATCT+CGG | 0.546722 | 1.1:-81581711 | None:intergenic |
| TTCATGCATGCATGCGCACT+AGG | 0.547297 | 1.1:+81582889 | MS.gene046124:CDS |
| TAGTATTTGACCTGTTCACC+AGG | 0.550072 | 1.1:-81585211 | None:intergenic |
| GCAGATTTCTTGAATCATGA+CGG | 0.555755 | 1.1:+81584594 | MS.gene046124:CDS |
| TTGTATGACAGGACTCTGAA+TGG | 0.556237 | 1.1:+81581657 | MS.gene046124:intron |
| AATTAATTGGATGAATAATG+AGG | 0.556334 | 1.1:-81592580 | None:intergenic |
| GCAAGTGCACGAAGTGATTG+TGG | 0.565446 | 1.1:-81592279 | None:intergenic |
| ATTATTCATCCAATTAATTG+CGG | 0.565451 | 1.1:+81592584 | MS.gene046124:CDS |
| CCTCCAACCAAAGATGCAAA+GGG | 0.571025 | 1.1:+81591569 | MS.gene046124:CDS |
| AACAGGAAGTCTTTCACTCG+AGG | 0.574466 | 1.1:-81592891 | None:intergenic |
| ATTATGCATCTCGGCTTGCG+GGG | 0.583027 | 1.1:-81581702 | None:intergenic |
| TGACGCAGAGGATCATACTT+AGG | 0.583198 | 1.1:-81591512 | None:intergenic |
| GTCTGCTGGAGGAAAAGGGA+AGG | 0.583307 | 1.1:+81592251 | MS.gene046124:CDS |
| TATTGATTTAGAAGCAAACA+AGG | 0.584991 | 1.1:-81581322 | None:intergenic |
| AAAACAGTAGTTCTCCAGCC+AGG | 0.585984 | 1.1:-81592975 | None:intergenic |
| GTACCTGGCCCAAATTCTTG+TGG | 0.590815 | 1.1:-81581558 | None:intergenic |
| ACGGAGGCTGCACAAACTGA+TGG | 0.603336 | 1.1:+81592495 | MS.gene046124:CDS |
| AAGTGCAAACTTGATTGTGA+AGG | 0.605155 | 1.1:-81591612 | None:intergenic |
| TAGAGCATGGGGAAGCACAA+AGG | 0.605458 | 1.1:+81584476 | MS.gene046124:CDS |
| AGAGCTTAACCATTTGGTCA+CGG | 0.610389 | 1.1:+81592476 | MS.gene046124:intron |
| AGGCTGCACAAACTGATGGT+AGG | 0.613383 | 1.1:+81592499 | MS.gene046124:CDS |
| TTATGCATCTCGGCTTGCGG+GGG | 0.622308 | 1.1:-81581701 | None:intergenic |
| CCAGTTGGCTCTAGAGCATG+GGG | 0.627133 | 1.1:+81584465 | MS.gene046124:intron |
| TGTTTACACACCCTGAAGAG+TGG | 0.628835 | 1.1:-81592319 | None:intergenic |
| ACTTCCTGTTAGAAGACGAA+TGG | 0.634423 | 1.1:+81592904 | MS.gene046124:CDS |
| TAAAAGGTTTGATTTCAGGA+GGG | 0.637802 | 1.1:-81581486 | None:intergenic |
| ATTTACTGACCATCAAGTCA+AGG | 0.654900 | 1.1:-81592999 | None:intergenic |
| TCTGCTGGAGGAAAAGGGAA+GGG | 0.677166 | 1.1:+81592252 | MS.gene046124:CDS |
| AGGGAGGTGAAGTCTGCTGG+AGG | 0.687293 | 1.1:+81592240 | MS.gene046124:intron |
| ATGGCTCGAGATCTCTTGCA+CGG | 0.704607 | 1.1:+81592923 | MS.gene046124:CDS |
| GCTTAACCATTTGGTCACGG+AGG | 0.739786 | 1.1:+81592479 | MS.gene046124:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 81581313 | 81593009 | 81581313 | ID=MS.gene046124 |
| chr1.1 | mRNA | 81581313 | 81593009 | 81581313 | ID=MS.gene046124.t1;Parent=MS.gene046124 |
| chr1.1 | exon | 81581313 | 81581351 | 81581313 | ID=MS.gene046124.t1.exon1;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81581313 | 81581351 | 81581313 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81581466 | 81581576 | 81581466 | ID=MS.gene046124.t1.exon2;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81581466 | 81581576 | 81581466 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81581668 | 81581727 | 81581668 | ID=MS.gene046124.t1.exon3;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81581668 | 81581727 | 81581668 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81581869 | 81581976 | 81581869 | ID=MS.gene046124.t1.exon4;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81581869 | 81581976 | 81581869 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81582841 | 81582910 | 81582841 | ID=MS.gene046124.t1.exon5;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81582841 | 81582910 | 81582841 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81584469 | 81584509 | 81584469 | ID=MS.gene046124.t1.exon6;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81584469 | 81584509 | 81584469 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81584585 | 81584665 | 81584585 | ID=MS.gene046124.t1.exon7;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81584585 | 81584665 | 81584585 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81585187 | 81585222 | 81585187 | ID=MS.gene046124.t1.exon8;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81585187 | 81585222 | 81585187 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81591261 | 81591344 | 81591261 | ID=MS.gene046124.t1.exon9;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81591261 | 81591344 | 81591261 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81591491 | 81591624 | 81591491 | ID=MS.gene046124.t1.exon10;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81591491 | 81591624 | 81591491 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81592242 | 81592330 | 81592242 | ID=MS.gene046124.t1.exon11;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81592242 | 81592330 | 81592242 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81592478 | 81592626 | 81592478 | ID=MS.gene046124.t1.exon12;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81592478 | 81592626 | 81592478 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| chr1.1 | exon | 81592866 | 81593009 | 81592866 | ID=MS.gene046124.t1.exon13;Parent=MS.gene046124.t1 |
| chr1.1 | CDS | 81592866 | 81593009 | 81592866 | ID=cds.MS.gene046124.t1;Parent=MS.gene046124.t1 |
| Gene Sequence |
| Protein sequence |
