AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene046218

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046218.t1 MTR_0402s0040 64.500 200 62 2 12 203 2 200 4.46e-75 253
MS.gene046218.t1 MTR_0402s0040 50.526 285 71 3 388 672 200 414 2.70e-71 243
MS.gene046218.t1 MTR_5g015480 64.848 165 54 1 1 165 25 185 3.35e-62 211
MS.gene046218.t1 MTR_0137s0040 61.078 167 11 2 1 167 1 113 2.74e-53 181
MS.gene046218.t1 MTR_6g017200 72.727 121 33 0 12 132 34 154 5.85e-52 178
MS.gene046218.t1 MTR_2g070590 71.552 116 33 0 24 139 5 120 5.89e-50 172
MS.gene046218.t1 MTR_1g057920 68.800 125 37 1 1 125 1 123 5.95e-49 169
MS.gene046218.t1 MTR_7g113010 55.901 161 55 2 1 161 424 568 1.36e-44 170
MS.gene046218.t1 MTR_0137s0070 47.475 198 78 6 673 846 435 630 1.83e-39 155
MS.gene046218.t1 MTR_1g086270 75.610 82 20 0 323 404 1 82 6.16e-38 137
MS.gene046218.t1 MTR_0137s0070 41.202 233 76 6 673 846 435 665 1.01e-36 148
MS.gene046218.t1 MTR_3g014080 46.465 198 80 6 673 846 1017 1212 1.28e-36 149
MS.gene046218.t1 MTR_6g051425 30.272 294 175 7 43 309 24 314 1.04e-34 136
MS.gene046218.t1 MTR_0558s0020 32.389 247 140 6 50 269 50 296 2.12e-32 128
MS.gene046218.t1 MTR_3g014080 39.662 237 74 9 673 846 1017 1247 9.92e-31 130
MS.gene046218.t1 MTR_8g446020 34.091 176 107 2 103 269 81 256 1.77e-26 109
MS.gene046218.t1 MTR_1g070355 31.535 241 151 6 35 263 71 309 6.06e-25 110
MS.gene046218.t1 MTR_6g090505 35.838 173 102 2 105 268 58 230 2.13e-24 106
MS.gene046218.t1 MTR_0291s0060 65.753 73 25 0 14 86 13 85 9.86e-24 96.3
MS.gene046218.t1 MTR_0137s0010 39.779 181 86 6 684 846 563 738 7.59e-22 101
MS.gene046218.t1 MTR_3g014040 60.241 83 32 1 765 846 1134 1216 2.78e-21 100
MS.gene046218.t1 MTR_1g050985 68.333 60 19 0 106 165 39 98 1.59e-20 87.8
MS.gene046218.t1 MTR_5g039760 35.714 196 104 7 32 224 6 182 8.87e-20 88.6
MS.gene046218.t1 MTR_4g067290 41.481 135 66 5 36 163 56 184 1.38e-19 89.4
MS.gene046218.t1 MTR_4g067290 41.481 135 66 5 36 163 76 204 1.75e-19 89.4
MS.gene046218.t1 MTR_4g067290 44.860 107 53 3 36 140 56 158 1.78e-19 89.0
MS.gene046218.t1 MTR_4g067290 41.481 135 66 5 36 163 74 202 1.88e-19 89.4
MS.gene046218.t1 MTR_3g007190 40.260 154 74 4 693 845 932 1068 2.65e-19 94.0
MS.gene046218.t1 MTR_4g067290 44.860 107 53 3 36 140 76 178 2.97e-19 89.0
MS.gene046218.t1 MTR_4g067290 44.860 107 53 3 36 140 116 218 4.04e-19 89.4
MS.gene046218.t1 MTR_4g035820 27.586 290 178 9 8 273 43 324 6.79e-19 92.0
MS.gene046218.t1 MTR_1g083640 27.197 239 142 7 34 240 44 282 8.59e-19 88.2
MS.gene046218.t1 MTR_7g074790 36.723 177 78 7 33 198 7 160 2.52e-18 85.5
MS.gene046218.t1 MTR_7g446520 28.980 245 148 6 39 274 91 318 6.25e-18 86.7
MS.gene046218.t1 MTR_2g098920 37.121 132 72 5 11 140 60 182 1.72e-17 84.0
MS.gene046218.t1 MTR_4g102720 30.233 172 84 4 33 204 4 139 1.93e-17 82.8
MS.gene046218.t1 MTR_1g091013 66.071 56 19 0 49 104 14 69 2.65e-16 74.3
MS.gene046218.t1 MTR_3g014320 36.310 168 100 5 685 845 1174 1341 4.00e-16 84.0
MS.gene046218.t1 MTR_7g061980 39.496 119 68 3 28 145 2 117 7.22e-16 75.1
MS.gene046218.t1 MTR_3g021340 30.435 322 162 16 553 836 834 1131 2.49e-14 77.8
MS.gene046218.t1 MTR_6g084540 32.353 170 87 6 691 846 77 232 7.42e-14 72.4
MS.gene046218.t1 MTR_5g045210 38.462 91 56 0 560 650 20 110 8.11e-14 71.6
MS.gene046218.t1 MTR_3g006370 35.433 127 75 3 36 161 50 170 9.17e-14 72.0
MS.gene046218.t1 MTR_3g022400 36.170 188 91 8 670 836 955 1134 1.82e-13 75.1
MS.gene046218.t1 MTR_3g022940 32.287 223 108 10 641 838 935 1139 2.18e-13 74.7
MS.gene046218.t1 MTR_4g019360 35.088 171 84 5 685 839 983 1142 3.40e-13 74.3
MS.gene046218.t1 MTR_5g076070 25.221 226 157 6 42 257 59 282 4.28e-13 71.2
MS.gene046218.t1 MTR_3g014510 32.716 162 99 5 693 845 1158 1318 6.34e-13 73.6
MS.gene046218.t1 MTR_3g032340 32.749 171 84 5 693 845 1135 1292 7.04e-13 73.2
MS.gene046218.t1 MTR_3g033860 30.653 199 101 7 685 846 1054 1252 9.72e-13 72.8
MS.gene046218.t1 MTR_0337s0040 28.000 150 99 2 96 236 75 224 1.14e-12 68.9
MS.gene046218.t1 MTR_3g035815 54.688 64 28 1 776 838 57 120 1.22e-12 65.9
MS.gene046218.t1 MTR_1g019850 30.460 174 110 4 4 174 36 201 1.32e-12 68.9
MS.gene046218.t1 MTR_3g022570 35.638 188 92 8 670 836 996 1175 2.52e-12 71.6
MS.gene046218.t1 MTR_2g099430 39.506 81 47 1 59 139 26 104 2.67e-12 64.7
MS.gene046218.t1 MTR_3g021400 31.064 235 124 11 624 836 912 1130 2.73e-12 71.2
MS.gene046218.t1 MTR_3g021100 34.574 188 92 10 673 838 969 1147 4.34e-12 70.9
MS.gene046218.t1 MTR_3g435220 44.444 90 41 2 757 845 1114 1195 8.91e-12 69.7
MS.gene046218.t1 MTR_2g098880 36.607 112 62 3 36 142 61 168 9.79e-12 66.6
MS.gene046218.t1 MTR_3g018930 34.211 190 94 11 672 839 972 1152 1.06e-11 69.3
MS.gene046218.t1 MTR_2g098880 36.607 112 62 3 36 142 73 180 1.14e-11 66.6
MS.gene046218.t1 MTR_0337s0060 26.190 168 95 2 419 583 21 162 1.16e-11 66.2
MS.gene046218.t1 MTR_7g446480 30.114 176 112 3 112 277 140 314 1.73e-11 66.6
MS.gene046218.t1 MTR_7g446510 29.545 176 113 4 112 277 155 329 1.83e-11 67.0
MS.gene046218.t1 MTR_1g045960 33.136 169 83 5 693 845 1047 1201 2.42e-11 68.2
MS.gene046218.t1 MTR_3g021380 35.119 168 90 6 686 839 968 1130 3.39e-11 67.8
MS.gene046218.t1 MTR_3g032110 44.565 92 36 3 757 845 1208 1287 5.92e-11 67.0
MS.gene046218.t1 MTR_3g022400 35.032 157 87 6 695 836 950 1106 6.33e-11 67.0
MS.gene046218.t1 MTR_3g435210 53.846 65 29 1 773 836 864 928 9.64e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046218.t1 AT4G38180 34.375 640 392 10 31 649 68 700 2.61e-114 367
MS.gene046218.t1 AT4G15090 29.538 650 429 9 17 645 25 666 1.91e-90 304
MS.gene046218.t1 AT4G15090 29.538 650 429 9 17 645 25 666 1.91e-90 304
MS.gene046218.t1 AT4G15090 29.538 650 429 9 17 645 25 666 1.91e-90 304
MS.gene046218.t1 AT4G15090 29.538 650 429 9 17 645 25 666 1.91e-90 304
MS.gene046218.t1 AT4G15090 29.538 650 429 9 17 645 25 666 1.91e-90 304
MS.gene046218.t1 AT3G22170 31.644 651 390 15 6 628 34 657 6.85e-90 303
MS.gene046218.t1 AT3G22170 31.644 651 390 15 6 628 34 657 6.85e-90 303
MS.gene046218.t1 AT1G76320 31.715 618 383 15 40 640 1 596 5.58e-86 288
MS.gene046218.t1 AT1G76320 31.715 618 383 15 40 640 1 596 3.06e-85 288
MS.gene046218.t1 AT1G76320 31.715 618 383 15 40 640 1 596 3.06e-85 288
MS.gene046218.t1 AT1G76320 31.715 618 383 15 40 640 1 596 3.12e-85 288
MS.gene046218.t1 AT2G27110 31.260 627 389 14 36 652 49 643 5.40e-82 281
MS.gene046218.t1 AT2G27110 31.260 627 389 14 36 652 49 643 5.40e-82 281
MS.gene046218.t1 AT3G06250 28.943 615 381 14 36 640 190 758 2.30e-74 258
MS.gene046218.t1 AT3G06250 28.943 615 381 14 36 640 190 758 2.30e-74 258
MS.gene046218.t1 AT3G06250 28.943 615 381 14 36 640 190 758 2.30e-74 258
MS.gene046218.t1 AT5G18960 29.167 624 387 15 28 640 203 782 6.69e-73 255
MS.gene046218.t1 AT5G18960 31.098 164 94 4 12 173 22 168 2.47e-12 71.2
MS.gene046218.t1 AT5G18960 29.167 624 387 15 28 640 203 782 6.69e-73 255
MS.gene046218.t1 AT5G18960 31.098 164 94 4 12 173 22 168 2.47e-12 71.2
MS.gene046218.t1 AT2G27110 33.333 453 286 8 207 652 55 498 1.53e-71 249
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 2.34e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 1 564 2.52e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 1 564 2.71e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 5.54e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 5.54e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 5.54e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 6.30e-69 244
MS.gene046218.t1 AT2G32250 26.974 608 393 10 40 640 42 605 6.30e-69 244
MS.gene046218.t1 AT1G10240 27.241 569 392 9 29 580 41 604 5.27e-64 228
MS.gene046218.t1 AT1G10240 27.241 569 392 9 29 580 41 604 5.27e-64 228
MS.gene046218.t1 AT4G38170 28.454 485 318 10 212 684 27 494 1.56e-63 223
MS.gene046218.t1 AT4G19990 27.787 619 392 10 41 638 26 610 1.12e-62 226
MS.gene046218.t1 AT4G19990 27.787 619 392 10 41 638 26 610 1.12e-62 226
MS.gene046218.t1 AT4G19990 27.787 619 392 10 41 638 26 610 1.20e-62 226
MS.gene046218.t1 AT4G19990 27.787 619 392 10 41 638 40 624 1.32e-62 226
MS.gene046218.t1 AT4G19990 27.111 675 418 13 41 682 26 659 1.83e-62 225
MS.gene046218.t1 AT4G19990 27.111 675 418 13 41 682 26 659 1.83e-62 225
MS.gene046218.t1 AT5G28530 26.131 597 402 13 34 603 53 637 6.12e-56 205
MS.gene046218.t1 AT5G28530 26.131 597 402 13 34 603 53 637 6.12e-56 205
MS.gene046218.t1 AT5G28530 25.667 600 405 12 34 603 53 641 1.53e-55 204
MS.gene046218.t1 AT4G19990 27.350 585 370 10 75 638 3 553 4.57e-55 203
MS.gene046218.t1 AT1G80010 26.576 587 372 13 36 583 95 661 6.63e-53 197
MS.gene046218.t1 AT1G52520 28.746 574 361 16 35 581 84 636 1.11e-52 196
MS.gene046218.t1 AT5G28530 25.301 581 364 17 34 603 53 574 1.18e-38 153
MS.gene046218.t1 AT5G28530 24.829 584 367 16 34 603 53 578 2.45e-38 152
MS.gene046218.t1 AT3G59470 39.706 136 72 4 36 165 70 201 6.26e-19 87.8
MS.gene046218.t1 AT3G59470 39.706 136 72 4 36 165 70 201 6.26e-19 87.8
MS.gene046218.t1 AT3G59470 39.416 137 73 4 36 166 82 214 7.94e-19 87.8
MS.gene046218.t1 AT3G07500 36.429 140 85 3 1 140 1 136 2.04e-15 76.6
MS.gene046218.t1 AT2G43280 42.857 105 56 3 36 138 22 124 6.94e-14 72.0
MS.gene046218.t1 AT4G12850 33.645 107 67 2 36 142 11 113 5.90e-12 64.3
MS.gene046218.t1 AT4G12850 33.628 113 71 2 36 148 11 119 9.43e-12 63.9
MS.gene046218.t1 AT4G12850 33.645 107 67 2 36 142 11 113 2.78e-11 63.9
MS.gene046218.t1 AT2G43280 42.222 90 48 3 51 138 2 89 5.10e-11 62.8

Find 160 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAATGTATTT+TGG 0.084293 3.3:+9128484 MS.gene046218:CDS
TATTATTTAAAATGCTTAAA+AGG 0.102888 3.3:+9130639 MS.gene046218:CDS
ATGAATTCCTTGATGAAAAT+TGG 0.174841 3.3:+9130556 MS.gene046218:CDS
TAAATAATAAAGATGAAAAT+TGG 0.226820 3.3:-9130625 None:intergenic
TTCTCTACACCTTCAACTAT+TGG 0.269471 3.3:-9127814 None:intergenic
TTTGGTATTCGTCGAGAATA+TGG 0.287007 3.3:+9127976 MS.gene046218:CDS
CACAATCCATGAAATGTTTC+CGG 0.296465 3.3:-9128789 None:intergenic
GCACAAATGATAAACGATTA+TGG 0.303139 3.3:+9128513 MS.gene046218:CDS
TTGAACTCTCTTGATATAGA+TGG 0.314695 3.3:-9128851 None:intergenic
AATAAGTATAGCAGGAGAAT+TGG 0.321280 3.3:+9127952 MS.gene046218:CDS
AGTTGAAGGTGTAGAGAAAT+TGG 0.324040 3.3:+9127819 MS.gene046218:CDS
TTGCATGTTGATCAGTTAGA+AGG 0.329715 3.3:-9128737 None:intergenic
AATAAGGAGCGGACGGTAAT+GGG 0.340111 3.3:+9129371 MS.gene046218:CDS
CATTTCATGGATTGTGCACT+TGG 0.341154 3.3:+9128796 MS.gene046218:CDS
ATTGGGAAGTAATCAAGATT+TGG 0.341191 3.3:+9129544 MS.gene046218:CDS
ATAATGTTTCTGAAGATTCA+TGG 0.342573 3.3:+9128961 MS.gene046218:CDS
CGCCCGTTGGGAGTATTTGT+TGG 0.343808 3.3:+9128591 MS.gene046218:CDS
GTTGTAGCAGCGGACGAATC+TGG 0.343821 3.3:+9128276 MS.gene046218:CDS
TTGGTTATCTTCGAACTAAA+AGG 0.348624 3.3:+9128358 MS.gene046218:CDS
AAGATTGGGACAAGCATATA+TGG 0.353381 3.3:-9130302 None:intergenic
ATTTCACTTGATCGAAAGAT+TGG 0.360362 3.3:+9128141 MS.gene046218:CDS
TGTCGATTATACACCACATT+TGG 0.361855 3.3:+9127888 MS.gene046218:CDS
ACCTTGAGATTTCAGATTGT+CGG 0.364901 3.3:+9130694 MS.gene046218:CDS
GACGGTAATGGGGAATCTTA+TGG 0.365249 3.3:+9129382 MS.gene046218:CDS
TACAAATTATGATAATAATA+AGG 0.368461 3.3:+9129355 MS.gene046218:CDS
CCCTCCTTGCTCTGACAGCT+TGG 0.376183 3.3:-9130793 None:intergenic
ACGTGCATCACATCCCGTTC+TGG 0.380300 3.3:-9128112 None:intergenic
TCAAATAACCATTGAAAAGA+AGG 0.380370 3.3:-9128675 None:intergenic
TACGACATACATGACAAATA+AGG 0.385236 3.3:+9128563 MS.gene046218:CDS
TTGATCAGTTTAACCATTTG+AGG 0.392360 3.3:-9129629 None:intergenic
TTTGAAGAAAGGGATGACTA+TGG 0.395864 3.3:-9130273 None:intergenic
GGCTATTTCTTTAGTTATGC+CGG 0.399841 3.3:+9128770 MS.gene046218:CDS
TCTGGATGTTGGTGGGGAGC+AGG 0.410929 3.3:+9129823 MS.gene046218:CDS
ATGGACAAGAGATGCAAGAT+TGG 0.411222 3.3:+9129526 MS.gene046218:CDS
GTTATGCCGGAAACATTTCA+TGG 0.413868 3.3:+9128783 MS.gene046218:CDS
TGCCTAAATTGAAAATGAAA+AGG 0.417192 3.3:+9129210 MS.gene046218:CDS
ATAAGTATAGCAGGAGAATT+GGG 0.417341 3.3:+9127953 MS.gene046218:CDS
TTTGCTCTTCAACATCCATT+TGG 0.421736 3.3:-9128467 None:intergenic
GTGCTTTAAAGAGGGGAAAC+GGG 0.422426 3.3:+9128044 MS.gene046218:CDS
GAGGAAGGCAGTCCTTCTTC+TGG 0.424904 3.3:-9130732 None:intergenic
GGAATTTCAAGAAGAATATG+AGG 0.425849 3.3:+9129283 MS.gene046218:CDS
TTATACACCACATTTGGAAA+TGG 0.427733 3.3:+9127894 MS.gene046218:CDS
ATGGATAAAGTTTGAAGAAA+GGG 0.431041 3.3:-9130283 None:intergenic
TAATAAGGAGCGGACGGTAA+TGG 0.433456 3.3:+9129370 MS.gene046218:CDS
TCATTGCTTCCGACTATATG+AGG 0.433888 3.3:-9130600 None:intergenic
CTCATGTCTTGACTCATAAT+CGG 0.441200 3.3:-9129186 None:intergenic
ATTATTTAAAATGCTTAAAA+GGG 0.445119 3.3:+9130640 MS.gene046218:CDS
CTGGATGTTGGTGGGGAGCA+GGG 0.451018 3.3:+9129824 MS.gene046218:CDS
GGGGAATCTTATGGATCAAA+AGG 0.451072 3.3:+9129391 MS.gene046218:CDS
ATGTGAATATTGTGCAGAAT+GGG 0.456804 3.3:+9127854 MS.gene046218:CDS
CGTGCTTTAAAGAGGGGAAA+CGG 0.458025 3.3:+9128043 MS.gene046218:CDS
AGGCTAGACAGACTCTGAAT+TGG 0.459576 3.3:-9130357 None:intergenic
CATTGTTGATGCATTTCAAA+CGG 0.460056 3.3:+9128412 MS.gene046218:CDS
GAAGAATATGAGGAATATCT+AGG 0.460765 3.3:+9129293 MS.gene046218:CDS
TAAAATAATTGATGATATGT+TGG 0.463062 3.3:+9129712 MS.gene046218:CDS
AAGTGGAAATGACCGAAGAT+TGG 0.464602 3.3:-9130317 None:intergenic
TACTTCAGCTATCACTAGGC+GGG 0.470556 3.3:+9130484 MS.gene046218:intron
AGTGGAAATGACCGAAGATT+GGG 0.474301 3.3:-9130316 None:intergenic
TGCGTAAAGAATTTGAAAGA+AGG 0.475897 3.3:+9129320 MS.gene046218:CDS
TGGAAGTCATAAAAGAATGA+AGG 0.477032 3.3:-9128447 None:intergenic
TGGACAAGAGATGCAAGATT+GGG 0.479430 3.3:+9129527 MS.gene046218:CDS
TTCCATGGACAACCCTTTCA+AGG 0.482368 3.3:-9129142 None:intergenic
TATATGCTTGTCCCAATCTT+CGG 0.484504 3.3:+9130305 MS.gene046218:CDS
TATGGATAAAGTTTGAAGAA+AGG 0.485878 3.3:-9130284 None:intergenic
AGGCAGTCCTTCTTCTGGTA+AGG 0.487880 3.3:-9130727 None:intergenic
CAACGAGTTATGTCCTCAAA+TGG 0.488180 3.3:+9129616 MS.gene046218:CDS
ATTCATTGTTCCGTATTCCA+TGG 0.490621 3.3:-9129157 None:intergenic
AATAAGGATTATCGCCCGTT+GGG 0.491675 3.3:+9128579 MS.gene046218:CDS
CAAATAACCATTGAAAAGAA+GGG 0.495588 3.3:-9128674 None:intergenic
CGAGACCACTTGACAAAAGA+GGG 0.501200 3.3:+9128078 MS.gene046218:CDS
GTCCAACAAATACTCCCAAC+GGG 0.502839 3.3:-9128593 None:intergenic
AAAGGGTTGTCCATGGAATA+CGG 0.503140 3.3:+9129147 MS.gene046218:CDS
GGAAGGGCAACCTATCGAAC+TGG 0.504370 3.3:+9129565 MS.gene046218:CDS
GCCTAAATTGAAAATGAAAA+GGG 0.504520 3.3:+9129211 MS.gene046218:CDS
GCGTAAAGAATTTGAAAGAA+GGG 0.512208 3.3:+9129321 MS.gene046218:CDS
AAGATTCACGTGCTTTAAAG+AGG 0.515507 3.3:+9128035 MS.gene046218:CDS
TATAGCAGGAGAATTGGGTT+TGG 0.515906 3.3:+9127958 MS.gene046218:CDS
TTGTTATCAGTAATTATGCA+TGG 0.520487 3.3:-9130843 None:intergenic
AACGGGATGTGATGCACGTA+TGG 0.520689 3.3:+9128116 MS.gene046218:CDS
GTTCGAAAACATTTCACTGC+AGG 0.524411 3.3:+9129029 MS.gene046218:CDS
TGTCATGTATCCATACCAAT+TGG 0.531415 3.3:+9129770 MS.gene046218:CDS
AGGATGCGGTCACTAATGTA+TGG 0.533492 3.3:+9128378 MS.gene046218:CDS
ACGAGACCACTTGACAAAAG+AGG 0.535505 3.3:+9128077 MS.gene046218:CDS
GATGTGAATATTGTGCAGAA+TGG 0.537847 3.3:+9127853 MS.gene046218:CDS
TCAAACTCCATTTCCAAATG+TGG 0.542045 3.3:-9127901 None:intergenic
TCAACAATTCCAATAGTTGA+AGG 0.542316 3.3:+9127805 MS.gene046218:CDS
CTTGAATCCTTACCAGAAGA+AGG 0.545255 3.3:+9130720 MS.gene046218:CDS
TTTCCGGCTTGTACAAGCAT+AGG 0.546185 3.3:-9129236 None:intergenic
TCGAAAGATTGGTAAGTACA+AGG 0.548048 3.3:+9128152 MS.gene046218:CDS
ATGCATTGATCTACATGAAG+AGG 0.551822 3.3:+9128896 MS.gene046218:CDS
GGAAATGGAGTTTGAATCTG+AGG 0.555015 3.3:+9127909 MS.gene046218:CDS
TGCATGTTGATCAGTTAGAA+GGG 0.557107 3.3:-9128736 None:intergenic
AGATTCACGTGCTTTAAAGA+GGG 0.557704 3.3:+9128036 MS.gene046218:CDS
GGAAGTAATCAAGATTTGGA+AGG 0.559886 3.3:+9129548 MS.gene046218:CDS
GTCACTAATGTATGGTGAAG+TGG 0.561332 3.3:+9128386 MS.gene046218:CDS
CACCTTGAAAGGGTTGTCCA+TGG 0.564856 3.3:+9129140 MS.gene046218:CDS
TTCTTCTTGAAATTCCTCAA+AGG 0.565966 3.3:-9129276 None:intergenic
CGCTTCAGTATATAGTGTTG+AGG 0.567827 3.3:-9129503 None:intergenic
AAATAAGGATTATCGCCCGT+TGG 0.568611 3.3:+9128578 MS.gene046218:CDS
GTCACTACTGAAAGAGAGGA+AGG 0.569317 3.3:-9130747 None:intergenic
GCTCCTATGCTTGTACAAGC+CGG 0.569380 3.3:+9129233 MS.gene046218:CDS
AACTGATCAACATGCAACAA+TGG 0.569412 3.3:+9128743 MS.gene046218:CDS
AACACTATATACTGAAGCGA+TGG 0.569686 3.3:+9129507 MS.gene046218:CDS
GGGAGAGTAGAAACCAGAAC+GGG 0.572783 3.3:+9128099 MS.gene046218:CDS
CCAAGCTGTCAGAGCAAGGA+GGG 0.581065 3.3:+9130793 MS.gene046218:CDS
TCCGACTTCCCTCATATAGT+CGG 0.581215 3.3:+9130591 MS.gene046218:CDS
GGAGTTTGAATCTGAGGCAG+CGG 0.581564 3.3:+9127915 MS.gene046218:CDS
TGACAACAATGTCAACACTC+TGG 0.582033 3.3:+9129805 MS.gene046218:CDS
TGACTCAGATATTTGTCGAT+GGG 0.583735 3.3:-9128241 None:intergenic
TGTCATGTATGTCGTATCAA+AGG 0.583944 3.3:-9128556 None:intergenic
TCCGACTATATGAGGGAAGT+CGG 0.586170 3.3:-9130592 None:intergenic
GCCGACAATCTGAAATCTCA+AGG 0.586350 3.3:-9130695 None:intergenic
TCAACATGCAACAATGGCTA+AGG 0.587790 3.3:+9128749 MS.gene046218:CDS
TTGAAGAAAGGGATGACTAT+GGG 0.588421 3.3:-9130272 None:intergenic
ATTCACAACGCTGTAAGACA+AGG 0.589456 3.3:-9130517 None:intergenic
TCACTAATGTATGGTGAAGT+GGG 0.590425 3.3:+9128387 MS.gene046218:CDS
ACTAAATAATCACAAGCAGA+TGG 0.591874 3.3:+9128614 MS.gene046218:CDS
GAAGTAATCAAGATTTGGAA+GGG 0.592932 3.3:+9129549 MS.gene046218:CDS
TGTCTACTTCAGCTATCACT+AGG 0.593950 3.3:+9130480 MS.gene046218:intron
GTGACTCAGATATTTGTCGA+TGG 0.600504 3.3:-9128242 None:intergenic
ATCTTCGAACTAAAAGGATG+CGG 0.601988 3.3:+9128364 MS.gene046218:CDS
ATAAACGATTATGGATACTT+CGG 0.603159 3.3:+9128522 MS.gene046218:CDS
CGAGGAGTATGAAAATGCCC+TGG 0.603338 3.3:-9128216 None:intergenic
GATAGTCACTACTGAAAGAG+AGG 0.606713 3.3:-9130751 None:intergenic
TGATAATAATAAGGAGCGGA+CGG 0.606864 3.3:+9129364 MS.gene046218:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 3.3:+9129425 MS.gene046218:CDS
TCAACACTCTGGATGTTGGT+GGG 0.611788 3.3:+9129816 MS.gene046218:CDS
GGGGAGAGTAGAAACCAGAA+CGG 0.611921 3.3:+9128098 MS.gene046218:CDS
ACTTCAGCTATCACTAGGCG+GGG 0.613507 3.3:+9130485 MS.gene046218:intron
TGCTTTAAAGAGGGGAAACG+GGG 0.614579 3.3:+9128045 MS.gene046218:CDS
GATTCACGTGCTTTAAAGAG+GGG 0.621094 3.3:+9128037 MS.gene046218:CDS
ACAACCAAGCTGTCAGAGCA+AGG 0.623605 3.3:+9130789 MS.gene046218:CDS
GTGAATATTGTGCAGAATGG+GGG 0.623992 3.3:+9127856 MS.gene046218:CDS
AGTCCAACAAATACTCCCAA+CGG 0.624240 3.3:-9128594 None:intergenic
TGAGCATTCAGTCTATGAAG+TGG 0.626486 3.3:-9130334 None:intergenic
CAACACTCTGGATGTTGGTG+GGG 0.627596 3.3:+9129817 MS.gene046218:CDS
CATTGCTTCCGACTATATGA+GGG 0.629446 3.3:-9130599 None:intergenic
CTACTTCAGCTATCACTAGG+CGG 0.632866 3.3:+9130483 MS.gene046218:intron
GTCAACACTCTGGATGTTGG+TGG 0.637024 3.3:+9129815 MS.gene046218:CDS
ATGTGTACAAGCAAGATGGT+GGG 0.637123 3.3:+9128325 MS.gene046218:CDS
CAGAGCAAGGAGGGAACGAG+TGG 0.638796 3.3:+9130802 MS.gene046218:CDS
TGTATCCATACCAATTGGCA+CGG 0.642140 3.3:+9129775 MS.gene046218:CDS
GATATTTGTCGATGGGAGCG+AGG 0.642651 3.3:-9128234 None:intergenic
TTGAGATTTCAGATTGTCGG+CGG 0.649583 3.3:+9130697 MS.gene046218:CDS
ATAAGGAGCGGACGGTAATG+GGG 0.649896 3.3:+9129372 MS.gene046218:CDS
TGTGAATATTGTGCAGAATG+GGG 0.650690 3.3:+9127855 MS.gene046218:CDS
AATGTCAACACTCTGGATGT+TGG 0.652365 3.3:+9129812 MS.gene046218:CDS
TATGTGTACAAGCAAGATGG+TGG 0.654711 3.3:+9128324 MS.gene046218:CDS
GAGTATGTGTACAAGCAAGA+TGG 0.660702 3.3:+9128321 MS.gene046218:CDS
ATTGATCTACATGAAGAGGA+AGG 0.663372 3.3:+9128900 MS.gene046218:CDS
ATATAAAAGCTCATTTCATG+AGG 0.670840 3.3:+9129588 MS.gene046218:CDS
ATTATCTCAAGTTGTAGCAG+CGG 0.678511 3.3:+9128266 MS.gene046218:CDS
AATACGGAACAATGAATCAG+AGG 0.679671 3.3:+9129163 MS.gene046218:CDS
TTCACAACGCTGTAAGACAA+GGG 0.681775 3.3:-9130516 None:intergenic
ATTATGATAATAATAAGGAG+CGG 0.687685 3.3:+9129360 MS.gene046218:CDS
GATGGTGGGATAGATGCTGT+TGG 0.699225 3.3:+9128339 MS.gene046218:CDS
GCGTTGTGAATTCGAGACGA+TGG 0.703580 3.3:+9130529 MS.gene046218:CDS
ACCAAGCTGTCAGAGCAAGG+AGG 0.707203 3.3:+9130792 MS.gene046218:CDS
GGAGTATGAAAATGCCCTGG+CGG 0.712650 3.3:-9128213 None:intergenic
GAGACCACTTGACAAAAGAG+GGG 0.716173 3.3:+9128079 MS.gene046218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATAAAGATGAAAAT+TGG - chr3.3:9130628-9130647 None:intergenic 10.0%
!! TACAAATTATGATAATAATA+AGG + chr3.3:9129355-9129374 MS.gene046218:CDS 10.0%
!!! ATTATTTAAAATGCTTAAAA+GGG + chr3.3:9130640-9130659 MS.gene046218:CDS 10.0%
!!! TATTATTTAAAATGCTTAAA+AGG + chr3.3:9130639-9130658 MS.gene046218:CDS 10.0%
!! GAAAATTTACAATGAATTTA+AGG + chr3.3:9130429-9130448 MS.gene046218:intron 15.0%
!!! AATTTTATAATAAGTATAGC+AGG + chr3.3:9127944-9127963 MS.gene046218:CDS 15.0%
!!! TAAAATAATTGATGATATGT+TGG + chr3.3:9129712-9129731 MS.gene046218:CDS 15.0%
!! ATTATGATAATAATAAGGAG+CGG + chr3.3:9129360-9129379 MS.gene046218:CDS 20.0%
!!! AGCAAATAACAAATGTATTT+TGG + chr3.3:9128484-9128503 MS.gene046218:CDS 20.0%
!!! GCAAATAACAAATGTATTTT+GGG + chr3.3:9128485-9128504 MS.gene046218:CDS 20.0%
!!! TTTTCAATTGAAGGAAAAAT+GGG + chr3.3:9128995-9129014 MS.gene046218:CDS 20.0%
!!! TTTTTCAATTGAAGGAAAAA+TGG + chr3.3:9128994-9129013 MS.gene046218:CDS 20.0%
! ATAAACGATTATGGATACTT+CGG + chr3.3:9128522-9128541 MS.gene046218:CDS 25.0%
! ATATAAAAGCTCATTTCATG+AGG + chr3.3:9129588-9129607 MS.gene046218:CDS 25.0%
! ATGAATTCCTTGATGAAAAT+TGG + chr3.3:9130556-9130575 MS.gene046218:CDS 25.0%
! ATGGATAAAGTTTGAAGAAA+GGG - chr3.3:9130286-9130305 None:intergenic 25.0%
! ATTAGACTCTTCAAAAACTT+TGG - chr3.3:9129693-9129712 None:intergenic 25.0%
! CAAATAACCATTGAAAAGAA+GGG - chr3.3:9128677-9128696 None:intergenic 25.0%
! CCTTCAATTGAAAAATCATA+CGG - chr3.3:9128989-9129008 None:intergenic 25.0%
! CTCAAATAATGCTAAAAAAC+GGG + chr3.3:9129866-9129885 MS.gene046218:intron 25.0%
! GCCTAAATTGAAAATGAAAA+GGG + chr3.3:9129211-9129230 MS.gene046218:CDS 25.0%
! GCTCAAATAATGCTAAAAAA+CGG + chr3.3:9129865-9129884 MS.gene046218:intron 25.0%
! GGAAATAAAAGCAAAAAAGA+TGG + chr3.3:9127997-9128016 MS.gene046218:CDS 25.0%
! TATGGATAAAGTTTGAAGAA+AGG - chr3.3:9130287-9130306 None:intergenic 25.0%
! TCAAATAACCATTGAAAAGA+AGG - chr3.3:9128678-9128697 None:intergenic 25.0%
! TCAAATAATGCTAAAAAACG+GGG + chr3.3:9129867-9129886 MS.gene046218:intron 25.0%
! TGCCTAAATTGAAAATGAAA+AGG + chr3.3:9129210-9129229 MS.gene046218:CDS 25.0%
! TTGTTATCAGTAATTATGCA+TGG - chr3.3:9130846-9130865 None:intergenic 25.0%
!! ATAATGTTTCTGAAGATTCA+TGG + chr3.3:9128961-9128980 MS.gene046218:CDS 25.0%
!! ATTCTTTTATGACTTCCAAA+TGG + chr3.3:9128452-9128471 MS.gene046218:CDS 25.0%
AATAAGTATAGCAGGAGAAT+TGG + chr3.3:9127952-9127971 MS.gene046218:CDS 30.0%
ACTAAATAATCACAAGCAGA+TGG + chr3.3:9128614-9128633 MS.gene046218:CDS 30.0%
AGTAGACAAACTCTCAAAAT+AGG - chr3.3:9130468-9130487 None:intergenic 30.0%
ATAAGTATAGCAGGAGAATT+GGG + chr3.3:9127953-9127972 MS.gene046218:CDS 30.0%
ATGTGAATATTGTGCAGAAT+GGG + chr3.3:9127854-9127873 MS.gene046218:CDS 30.0%
ATTGGGAAGTAATCAAGATT+TGG + chr3.3:9129544-9129563 MS.gene046218:CDS 30.0%
ATTTCACTTGATCGAAAGAT+TGG + chr3.3:9128141-9128160 MS.gene046218:CDS 30.0%
CATACACACTCAATTGATAA+AGG - chr3.3:9130380-9130399 None:intergenic 30.0%
CATTGTTGATGCATTTCAAA+CGG + chr3.3:9128412-9128431 MS.gene046218:CDS 30.0%
CTTTATCAATTGAGTGTGTA+TGG + chr3.3:9130378-9130397 MS.gene046218:intron 30.0%
GAAGAATATGAGGAATATCT+AGG + chr3.3:9129293-9129312 MS.gene046218:CDS 30.0%
GAAGTAATCAAGATTTGGAA+GGG + chr3.3:9129549-9129568 MS.gene046218:CDS 30.0%
GATTCTCACAAGAAAGAAAA+AGG - chr3.3:9130079-9130098 None:intergenic 30.0%
GCACAAATGATAAACGATTA+TGG + chr3.3:9128513-9128532 MS.gene046218:CDS 30.0%
GCGTAAAGAATTTGAAAGAA+GGG + chr3.3:9129321-9129340 MS.gene046218:CDS 30.0%
GGAATTTCAAGAAGAATATG+AGG + chr3.3:9129283-9129302 MS.gene046218:CDS 30.0%
TACGACATACATGACAAATA+AGG + chr3.3:9128563-9128582 MS.gene046218:CDS 30.0%
TCAACAATTCCAATAGTTGA+AGG + chr3.3:9127805-9127824 MS.gene046218:CDS 30.0%
TGCGTAAAGAATTTGAAAGA+AGG + chr3.3:9129320-9129339 MS.gene046218:CDS 30.0%
TGGAAGTCATAAAAGAATGA+AGG - chr3.3:9128450-9128469 None:intergenic 30.0%
TTATACACCACATTTGGAAA+TGG + chr3.3:9127894-9127913 MS.gene046218:CDS 30.0%
TTCTTCTTGAAATTCCTCAA+AGG - chr3.3:9129279-9129298 None:intergenic 30.0%
TTGAACTCTCTTGATATAGA+TGG - chr3.3:9128854-9128873 None:intergenic 30.0%
TTGATCAGTTTAACCATTTG+AGG - chr3.3:9129632-9129651 None:intergenic 30.0%
TTGGTTATCTTCGAACTAAA+AGG + chr3.3:9128358-9128377 MS.gene046218:CDS 30.0%
! AAACAATCCCTTCTTTTCAA+TGG + chr3.3:9128667-9128686 MS.gene046218:CDS 30.0%
! AATTTTTCACACACCTTGAA+AGG + chr3.3:9129129-9129148 MS.gene046218:CDS 30.0%
! ATTTTTCACACACCTTGAAA+GGG + chr3.3:9129130-9129149 MS.gene046218:CDS 30.0%
! GAAACTTTTCTCAAAGCAAT+GGG + chr3.3:9128696-9128715 MS.gene046218:CDS 30.0%
! GCCCTTTTCATTTTCAATTT+AGG - chr3.3:9129215-9129234 None:intergenic 30.0%
! TGAAACTTTTCTCAAAGCAA+TGG + chr3.3:9128695-9128714 MS.gene046218:CDS 30.0%
! TGAAATTCCTCAAAGGTTTT+TGG - chr3.3:9129272-9129291 None:intergenic 30.0%
! TTCAAAAGTCTTTTCATCTC+AGG + chr3.3:9130208-9130227 MS.gene046218:intron 30.0%
!! CCGTATGATTTTTCAATTGA+AGG + chr3.3:9128986-9129005 MS.gene046218:CDS 30.0%
!! TGAAAAGACTTTTGAAGACT+AGG - chr3.3:9130204-9130223 None:intergenic 30.0%
!!! TTTTGGAGTGTAAATGTTTC+CGG - chr3.3:9129255-9129274 None:intergenic 30.0%
AACACTATATACTGAAGCGA+TGG + chr3.3:9129507-9129526 MS.gene046218:CDS 35.0%
AACTGATCAACATGCAACAA+TGG + chr3.3:9128743-9128762 MS.gene046218:CDS 35.0%
AAGATTGGGACAAGCATATA+TGG - chr3.3:9130305-9130324 None:intergenic 35.0%
AAGGAGAGATTGTAATGTTG+AGG - chr3.3:9130060-9130079 None:intergenic 35.0%
AAGTGAAGTGCAATATGGTA+AGG - chr3.3:9130123-9130142 None:intergenic 35.0%
AATACGGAACAATGAATCAG+AGG + chr3.3:9129163-9129182 MS.gene046218:CDS 35.0%
AATTGAAGGAAAAATGGGCA+TGG + chr3.3:9129000-9129019 MS.gene046218:CDS 35.0%
ACCTTGAGATTTCAGATTGT+CGG + chr3.3:9130694-9130713 MS.gene046218:CDS 35.0%
AGGAGAGATTGTAATGTTGA+GGG - chr3.3:9130059-9130078 None:intergenic 35.0%
ATAATGCTAAAAAACGGGGT+TGG + chr3.3:9129871-9129890 MS.gene046218:intron 35.0%
ATCTTCGAACTAAAAGGATG+CGG + chr3.3:9128364-9128383 MS.gene046218:CDS 35.0%
ATTCATTGTTCCGTATTCCA+TGG - chr3.3:9129160-9129179 None:intergenic 35.0%
ATTGAAGGAAAAATGGGCAT+GGG + chr3.3:9129001-9129020 MS.gene046218:CDS 35.0%
ATTGATCTACATGAAGAGGA+AGG + chr3.3:9128900-9128919 MS.gene046218:CDS 35.0%
CAATATGGTAAGGATTCATG+AGG - chr3.3:9130113-9130132 None:intergenic 35.0%
CACAATCCATGAAATGTTTC+CGG - chr3.3:9128792-9128811 None:intergenic 35.0%
CTCATGTCTTGACTCATAAT+CGG - chr3.3:9129189-9129208 None:intergenic 35.0%
GATGTGAATATTGTGCAGAA+TGG + chr3.3:9127853-9127872 MS.gene046218:CDS 35.0%
GGAAGTAATCAAGATTTGGA+AGG + chr3.3:9129548-9129567 MS.gene046218:CDS 35.0%
TATATGCTTGTCCCAATCTT+CGG + chr3.3:9130305-9130324 MS.gene046218:CDS 35.0%
TCAAACTCCATTTCCAAATG+TGG - chr3.3:9127904-9127923 None:intergenic 35.0%
TCACTAATGTATGGTGAAGT+GGG + chr3.3:9128387-9128406 MS.gene046218:CDS 35.0%
TCGAAAGATTGGTAAGTACA+AGG + chr3.3:9128152-9128171 MS.gene046218:CDS 35.0%
TGACTCAGATATTTGTCGAT+GGG - chr3.3:9128244-9128263 None:intergenic 35.0%
TGATAATAATAAGGAGCGGA+CGG + chr3.3:9129364-9129383 MS.gene046218:CDS 35.0%
TGCATGTTGATCAGTTAGAA+GGG - chr3.3:9128739-9128758 None:intergenic 35.0%
TGTCATGTATCCATACCAAT+TGG + chr3.3:9129770-9129789 MS.gene046218:CDS 35.0%
TGTCATGTATGTCGTATCAA+AGG - chr3.3:9128559-9128578 None:intergenic 35.0%
TGTGAATATTGTGCAGAATG+GGG + chr3.3:9127855-9127874 MS.gene046218:CDS 35.0%
TTACACTCCAAAAACCTTTG+AGG + chr3.3:9129262-9129281 MS.gene046218:CDS 35.0%
TTCTCAAGTGAAGTGCAATA+TGG - chr3.3:9130128-9130147 None:intergenic 35.0%
TTCTCTACACCTTCAACTAT+TGG - chr3.3:9127817-9127836 None:intergenic 35.0%
TTGAAGAAAGGGATGACTAT+GGG - chr3.3:9130275-9130294 None:intergenic 35.0%
TTGCATGTTGATCAGTTAGA+AGG - chr3.3:9128740-9128759 None:intergenic 35.0%
TTTGAAGAAAGGGATGACTA+TGG - chr3.3:9130276-9130295 None:intergenic 35.0%
TTTGCTCTTCAACATCCATT+TGG - chr3.3:9128470-9128489 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr3.3:9127976-9127995 MS.gene046218:CDS 35.0%
! AGTTGAAGGTGTAGAGAAAT+TGG + chr3.3:9127819-9127838 MS.gene046218:CDS 35.0%
! ATGCATTGATCTACATGAAG+AGG + chr3.3:9128896-9128915 MS.gene046218:CDS 35.0%
! ATGTTGGCTAAAAAGTTCGT+GGG + chr3.3:9129728-9129747 MS.gene046218:CDS 35.0%
! ATTATCTCAAGTTGTAGCAG+CGG + chr3.3:9128266-9128285 MS.gene046218:CDS 35.0%
! GCTTTTATATCCAGTTCGAT+AGG - chr3.3:9129578-9129597 None:intergenic 35.0%
! TATGTTGGCTAAAAAGTTCG+TGG + chr3.3:9129727-9129746 MS.gene046218:CDS 35.0%
! TGACTCTCCAATTTTCATCA+AGG - chr3.3:9130566-9130585 None:intergenic 35.0%
! TGATCAGTTAGAAGGGTTTT+AGG - chr3.3:9128732-9128751 None:intergenic 35.0%
! TGTCGATTATACACCACATT+TGG + chr3.3:9127888-9127907 MS.gene046218:CDS 35.0%
! TGTCGTAAATTTGAGACACA+TGG + chr3.3:9129425-9129444 MS.gene046218:CDS 35.0%
!! AAGATTCACGTGCTTTAAAG+AGG + chr3.3:9128035-9128054 MS.gene046218:CDS 35.0%
!! AGATTCACGTGCTTTAAAGA+GGG + chr3.3:9128036-9128055 MS.gene046218:CDS 35.0%
!! GGCTATTTCTTTAGTTATGC+CGG + chr3.3:9128770-9128789 MS.gene046218:CDS 35.0%
AAAGGGTTGTCCATGGAATA+CGG + chr3.3:9129147-9129166 MS.gene046218:CDS 40.0%
AAATAAGGATTATCGCCCGT+TGG + chr3.3:9128578-9128597 MS.gene046218:CDS 40.0%
AAGTGGAAATGACCGAAGAT+TGG - chr3.3:9130320-9130339 None:intergenic 40.0%
AATAAGGATTATCGCCCGTT+GGG + chr3.3:9128579-9128598 MS.gene046218:CDS 40.0%
AATGTCAACACTCTGGATGT+TGG + chr3.3:9129812-9129831 MS.gene046218:CDS 40.0%
AGTCCAACAAATACTCCCAA+CGG - chr3.3:9128597-9128616 None:intergenic 40.0%
AGTGGAAATGACCGAAGATT+GGG - chr3.3:9130319-9130338 None:intergenic 40.0%
ATGGACAAGAGATGCAAGAT+TGG + chr3.3:9129526-9129545 MS.gene046218:CDS 40.0%
ATGTGTACAAGCAAGATGGT+GGG + chr3.3:9128325-9128344 MS.gene046218:CDS 40.0%
ATTCACAACGCTGTAAGACA+AGG - chr3.3:9130520-9130539 None:intergenic 40.0%
CAACGAGTTATGTCCTCAAA+TGG + chr3.3:9129616-9129635 MS.gene046218:CDS 40.0%
CATTGCTTCCGACTATATGA+GGG - chr3.3:9130602-9130621 None:intergenic 40.0%
CGCTTCAGTATATAGTGTTG+AGG - chr3.3:9129506-9129525 None:intergenic 40.0%
CTTGAATCCTTACCAGAAGA+AGG + chr3.3:9130720-9130739 MS.gene046218:CDS 40.0%
GAGTATGTGTACAAGCAAGA+TGG + chr3.3:9128321-9128340 MS.gene046218:CDS 40.0%
GATAGTCACTACTGAAAGAG+AGG - chr3.3:9130754-9130773 None:intergenic 40.0%
GGAAATGGAGTTTGAATCTG+AGG + chr3.3:9127909-9127928 MS.gene046218:CDS 40.0%
GGAGAGATTGTAATGTTGAG+GGG - chr3.3:9130058-9130077 None:intergenic 40.0%
GGGGAATCTTATGGATCAAA+AGG + chr3.3:9129391-9129410 MS.gene046218:CDS 40.0%
GTCACTAATGTATGGTGAAG+TGG + chr3.3:9128386-9128405 MS.gene046218:CDS 40.0%
GTGAATATTGTGCAGAATGG+GGG + chr3.3:9127856-9127875 MS.gene046218:CDS 40.0%
GTGACTCAGATATTTGTCGA+TGG - chr3.3:9128245-9128264 None:intergenic 40.0%
GTGATTCATTGACATCCTCT+CGG + chr3.3:9130168-9130187 MS.gene046218:intron 40.0%
GTTATGCCGGAAACATTTCA+TGG + chr3.3:9128783-9128802 MS.gene046218:CDS 40.0%
GTTCGAAAACATTTCACTGC+AGG + chr3.3:9129029-9129048 MS.gene046218:CDS 40.0%
TATAGCAGGAGAATTGGGTT+TGG + chr3.3:9127958-9127977 MS.gene046218:CDS 40.0%
TATGTGTACAAGCAAGATGG+TGG + chr3.3:9128324-9128343 MS.gene046218:CDS 40.0%
TCAACATGCAACAATGGCTA+AGG + chr3.3:9128749-9128768 MS.gene046218:CDS 40.0%
TCATTGCTTCCGACTATATG+AGG - chr3.3:9130603-9130622 None:intergenic 40.0%
TGACAACAATGTCAACACTC+TGG + chr3.3:9129805-9129824 MS.gene046218:CDS 40.0%
TGAGCATTCAGTCTATGAAG+TGG - chr3.3:9130337-9130356 None:intergenic 40.0%
TGGACAAGAGATGCAAGATT+GGG + chr3.3:9129527-9129546 MS.gene046218:CDS 40.0%
TGTATCCATACCAATTGGCA+CGG + chr3.3:9129775-9129794 MS.gene046218:CDS 40.0%
TGTCTACTTCAGCTATCACT+AGG + chr3.3:9130480-9130499 MS.gene046218:intron 40.0%
TTCACAACGCTGTAAGACAA+GGG - chr3.3:9130519-9130538 None:intergenic 40.0%
TTGAGATTTCAGATTGTCGG+CGG + chr3.3:9130697-9130716 MS.gene046218:CDS 40.0%
TTGAGGGGAGAACATTTCTT+GGG - chr3.3:9130043-9130062 None:intergenic 40.0%
! AATCATTTTCTTGAACCGCC+AGG + chr3.3:9128198-9128217 MS.gene046218:CDS 40.0%
! ACTTTTCTCAAAGCAATGGG+TGG + chr3.3:9128699-9128718 MS.gene046218:CDS 40.0%
! ATCATTTTCTTGAACCGCCA+GGG + chr3.3:9128199-9128218 MS.gene046218:CDS 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr3.3:9128796-9128815 MS.gene046218:CDS 40.0%
! TGTTGGCTAAAAAGTTCGTG+GGG + chr3.3:9129729-9129748 MS.gene046218:CDS 40.0%
! TTTTTCCGTGCCAATTGGTA+TGG - chr3.3:9129783-9129802 None:intergenic 40.0%
!! AAGGTGTGTTTTTGAACGCA+TGG + chr3.3:9128919-9128938 MS.gene046218:CDS 40.0%
!! CACAAGCAGATGGTTGTTTT+TGG + chr3.3:9128624-9128643 MS.gene046218:CDS 40.0%
!! GATTCACGTGCTTTAAAGAG+GGG + chr3.3:9128037-9128056 MS.gene046218:CDS 40.0%
!!! TCACTTTTTTCCGTGCCAAT+TGG - chr3.3:9129788-9129807 None:intergenic 40.0%
AAAGACCATCACATCCTGAG+TGG + chr3.3:9129965-9129984 MS.gene046218:intron 45.0%
AATAAGGAGCGGACGGTAAT+GGG + chr3.3:9129371-9129390 MS.gene046218:CDS 45.0%
ACGAGACCACTTGACAAAAG+AGG + chr3.3:9128077-9128096 MS.gene046218:CDS 45.0%
AGGATGCGGTCACTAATGTA+TGG + chr3.3:9128378-9128397 MS.gene046218:CDS 45.0%
AGGCTAGACAGACTCTGAAT+TGG - chr3.3:9130360-9130379 None:intergenic 45.0%
AGTCTAATCACCATCCACTC+AGG - chr3.3:9129982-9130001 None:intergenic 45.0%
CGAGACCACTTGACAAAAGA+GGG + chr3.3:9128078-9128097 MS.gene046218:CDS 45.0%
CTACTTCAGCTATCACTAGG+CGG + chr3.3:9130483-9130502 MS.gene046218:intron 45.0%
GAGACCACTTGACAAAAGAG+GGG + chr3.3:9128079-9128098 MS.gene046218:CDS 45.0%
GTCACTACTGAAAGAGAGGA+AGG - chr3.3:9130750-9130769 None:intergenic 45.0%
GTCCAACAAATACTCCCAAC+GGG - chr3.3:9128596-9128615 None:intergenic 45.0%
GTTGAGGGGAGAACATTTCT+TGG - chr3.3:9130044-9130063 None:intergenic 45.0%
GTTGGCTAAAAAGTTCGTGG+GGG + chr3.3:9129730-9129749 MS.gene046218:CDS 45.0%
TAATAAGGAGCGGACGGTAA+TGG + chr3.3:9129370-9129389 MS.gene046218:CDS 45.0%
TACTTCAGCTATCACTAGGC+GGG + chr3.3:9130484-9130503 MS.gene046218:intron 45.0%
TCAACACTCTGGATGTTGGT+GGG + chr3.3:9129816-9129835 MS.gene046218:CDS 45.0%
TCCGACTATATGAGGGAAGT+CGG - chr3.3:9130595-9130614 None:intergenic 45.0%
TCCGACTTCCCTCATATAGT+CGG + chr3.3:9130591-9130610 MS.gene046218:CDS 45.0%
TTCCATGGACAACCCTTTCA+AGG - chr3.3:9129145-9129164 None:intergenic 45.0%
! GCCGACAATCTGAAATCTCA+AGG - chr3.3:9130698-9130717 None:intergenic 45.0%
! TTTCCGGCTTGTACAAGCAT+AGG - chr3.3:9129239-9129258 None:intergenic 45.0%
!! CGTGCTTTAAAGAGGGGAAA+CGG + chr3.3:9128043-9128062 MS.gene046218:CDS 45.0%
!! GACGGTAATGGGGAATCTTA+TGG + chr3.3:9129382-9129401 MS.gene046218:CDS 45.0%
!! GTGCTTTAAAGAGGGGAAAC+GGG + chr3.3:9128044-9128063 MS.gene046218:CDS 45.0%
!! TGCTTTAAAGAGGGGAAACG+GGG + chr3.3:9128045-9128064 MS.gene046218:CDS 45.0%
AACGGGATGTGATGCACGTA+TGG + chr3.3:9128116-9128135 MS.gene046218:CDS 50.0%
ACAACCAAGCTGTCAGAGCA+AGG + chr3.3:9130789-9130808 MS.gene046218:CDS 50.0%
ACCATCACATCCTGAGTGGA+TGG + chr3.3:9129969-9129988 MS.gene046218:intron 50.0%
ACCATCCACTCAGGATGTGA+TGG - chr3.3:9129973-9129992 None:intergenic 50.0%
ACTTCAGCTATCACTAGGCG+GGG + chr3.3:9130485-9130504 MS.gene046218:intron 50.0%
AGGCAGTCCTTCTTCTGGTA+AGG - chr3.3:9130730-9130749 None:intergenic 50.0%
ATAAGGAGCGGACGGTAATG+GGG + chr3.3:9129372-9129391 MS.gene046218:CDS 50.0%
CAACACTCTGGATGTTGGTG+GGG + chr3.3:9129817-9129836 MS.gene046218:CDS 50.0%
CACCTTGAAAGGGTTGTCCA+TGG + chr3.3:9129140-9129159 MS.gene046218:CDS 50.0%
GATATTTGTCGATGGGAGCG+AGG - chr3.3:9128237-9128256 None:intergenic 50.0%
GCGTTGTGAATTCGAGACGA+TGG + chr3.3:9130529-9130548 MS.gene046218:CDS 50.0%
GCTCCTATGCTTGTACAAGC+CGG + chr3.3:9129233-9129252 MS.gene046218:CDS 50.0%
GGAGTATGAAAATGCCCTGG+CGG - chr3.3:9128216-9128235 None:intergenic 50.0%
GGGAGAGTAGAAACCAGAAC+GGG + chr3.3:9128099-9128118 MS.gene046218:CDS 50.0%
GGGGAGAACATTTCTTGGGT+AGG - chr3.3:9130039-9130058 None:intergenic 50.0%
GGGGAGAGTAGAAACCAGAA+CGG + chr3.3:9128098-9128117 MS.gene046218:CDS 50.0%
GTAAATGACACTGCGACGAC+TGG - chr3.3:9129904-9129923 None:intergenic 50.0%
GTATGGACGTCGAAAACTGC+AGG + chr3.3:9130395-9130414 MS.gene046218:intron 50.0%
GTCAACACTCTGGATGTTGG+TGG + chr3.3:9129815-9129834 MS.gene046218:CDS 50.0%
! CAGGTCACAGCATCGAAAAG+AGG + chr3.3:9129925-9129944 MS.gene046218:intron 50.0%
! CGAGGAGTATGAAAATGCCC+TGG - chr3.3:9128219-9128238 None:intergenic 50.0%
! CTCTCCCCTCTTTTGTCAAG+TGG - chr3.3:9128086-9128105 None:intergenic 50.0%
! GGAGTTTGAATCTGAGGCAG+CGG + chr3.3:9127915-9127934 MS.gene046218:CDS 50.0%
!! CGTCGCAGTGTCATTTACTC+AGG + chr3.3:9129906-9129925 MS.gene046218:intron 50.0%
!! GATGGTGGGATAGATGCTGT+TGG + chr3.3:9128339-9128358 MS.gene046218:CDS 50.0%
ACCAAGCTGTCAGAGCAAGG+AGG + chr3.3:9130792-9130811 MS.gene046218:CDS 55.0%
ACGTGCATCACATCCCGTTC+TGG - chr3.3:9128115-9128134 None:intergenic 55.0%
CCAAGCTGTCAGAGCAAGGA+GGG + chr3.3:9130793-9130812 MS.gene046218:CDS 55.0%
CTAGGAGAGAACTAGCCGAG+AGG - chr3.3:9130186-9130205 None:intergenic 55.0%
GAGGAAGGCAGTCCTTCTTC+TGG - chr3.3:9130735-9130754 None:intergenic 55.0%
GGAAGGGCAACCTATCGAAC+TGG + chr3.3:9129565-9129584 MS.gene046218:CDS 55.0%
GTTGTAGCAGCGGACGAATC+TGG + chr3.3:9128276-9128295 MS.gene046218:CDS 55.0%
!! CGCCCGTTGGGAGTATTTGT+TGG + chr3.3:9128591-9128610 MS.gene046218:CDS 55.0%
CAGAGCAAGGAGGGAACGAG+TGG + chr3.3:9130802-9130821 MS.gene046218:CDS 60.0%
CCCTCCTTGCTCTGACAGCT+TGG - chr3.3:9130796-9130815 None:intergenic 60.0%
! CTGGATGTTGGTGGGGAGCA+GGG + chr3.3:9129824-9129843 MS.gene046218:CDS 60.0%
! TCTGGATGTTGGTGGGGAGC+AGG + chr3.3:9129823-9129842 MS.gene046218:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 9127796 9130878 9127796 ID=MS.gene046218
chr3.3 mRNA 9127796 9130878 9127796 ID=MS.gene046218.t1;Parent=MS.gene046218
chr3.3 exon 9127796 9129845 9127796 ID=MS.gene046218.t1.exon1;Parent=MS.gene046218.t1
chr3.3 CDS 9127796 9129845 9127796 ID=cds.MS.gene046218.t1;Parent=MS.gene046218.t1
chr3.3 exon 9130263 9130366 9130263 ID=MS.gene046218.t1.exon2;Parent=MS.gene046218.t1
chr3.3 CDS 9130263 9130366 9130263 ID=cds.MS.gene046218.t1;Parent=MS.gene046218.t1
chr3.3 exon 9130492 9130878 9130492 ID=MS.gene046218.t1.exon3;Parent=MS.gene046218.t1
chr3.3 CDS 9130492 9130878 9130492 ID=cds.MS.gene046218.t1;Parent=MS.gene046218.t1
Gene Sequence

>MS.gene046218

ATGAATGGGTCAACAATTCCAATAGTTGAAGGTGTAGAGAAATTGGTGAACAATATTGATGTGAATATTGTGCAGAATGGGGGAGAGAGTGTTGTCGATTATACACCACATTTGGAAATGGAGTTTGAATCTGAGGCAGCGGCATATGAATTTTATAATAAGTATAGCAGGAGAATTGGGTTTGGTATTCGTCGAGAATATGGAAATAAAAGCAAAAAAGATGGAATTCTGACTTCAAGAAGATTCACGTGCTTTAAAGAGGGGAAACGGGGTGTTGACAAACGAGACCACTTGACAAAAGAGGGGAGAGTAGAAACCAGAACGGGATGTGATGCACGTATGGTTATTTCACTTGATCGAAAGATTGGTAAGTACAAGGTTGTTGACTTTGTAGCTCAACATAATCATTTTCTTGAACCGCCAGGGCATTTTCATACTCCTCGCTCCCATCGACAAATATCTGAGTCACGATTATCTCAAGTTGTAGCAGCGGACGAATCTGGATTAAAACGAAAAGATTTTCATGAGTATGTGTACAAGCAAGATGGTGGGATAGATGCTGTTGGTTATCTTCGAACTAAAAGGATGCGGTCACTAATGTATGGTGAAGTGGGTGCATTGTTGATGCATTTCAAACGGCAAAGTGAAAATCCTTCATTCTTTTATGACTTCCAAATGGATGTTGAAGAGCAAATAACAAATGTATTTTGGGCAGATGCACAAATGATAAACGATTATGGATACTTCGGTGATGTCATCACCTTTGATACGACATACATGACAAATAAGGATTATCGCCCGTTGGGAGTATTTGTTGGACTAAATAATCACAAGCAGATGGTTGTTTTTGGTGCAACATTGCTATATGATGAAACAATCCCTTCTTTTCAATGGTTATTTGAAACTTTTCTCAAAGCAATGGGTGGAGAAAAGCCTAAAACCCTTCTAACTGATCAACATGCAACAATGGCTAAGGCTATTTCTTTAGTTATGCCGGAAACATTTCATGGATTGTGCACTTGGCGTATGAGAGAAAATGCACAGAGACATGTGAACCATCTATATCAAGAGAGTTCAAAATTCTGCTCAGATTTTGAAGCATGCATTGATCTACATGAAGAGGAAGGTGTGTTTTTGAACGCATGGAATGTTTTACTTGTCGAACATAATGTTTCTGAAGATTCATGGCTCCGTATGATTTTTCAATTGAAGGAAAAATGGGCATGGGCGTATGTTCGAAAACATTTCACTGCAGGTATGAGATCTACTCAAATAAGTGAGAGTTTTAATGCTGAGTTAAAAAATTATTTGAAGTCAGATTTAAATTTAGTTCAATTTTTCACACACCTTGAAAGGGTTGTCCATGGAATACGGAACAATGAATCAGAGGCCGATTATGAGTCAAGACATGAGTTGCCTAAATTGAAAATGAAAAGGGCTCCTATGCTTGTACAAGCCGGAAACATTTACACTCCAAAAACCTTTGAGGAATTTCAAGAAGAATATGAGGAATATCTAGGCACTTGCGTAAAGAATTTGAAAGAAGGGTTGTATGCTGTTACAAATTATGATAATAATAAGGAGCGGACGGTAATGGGGAATCTTATGGATCAAAAGGTTGTCTGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGCAGTCATGCTTTGAAAGTATTAGATGTTATGAATATTAAGCTGATTCCTCAACACTATATACTGAAGCGATGGACAAGAGATGCAAGATTGGGAAGTAATCAAGATTTGGAAGGGCAACCTATCGAACTGGATATAAAAGCTCATTTCATGAGGCGATACAACGAGTTATGTCCTCAAATGGTTAAACTGATCAACAAAGCATCAAAATCTCATGAAACTTACACATTTTTATCCAAAGTTTTTGAAGAGTCTAATAAAATAATTGATGATATGTTGGCTAAAAAGTTCGTGGGGGCAGAATCACTTGAGCTGTGTCATGTATCCATACCAATTGGCACGGAAAAAAGTGACAACAATGTCAACACTCTGGATGTTGGTGGGGAGCAGGGTATAAAGAAAGCAAGATTGCTCAAATAATGCTAAAAAACGGGGTTGGACATAATCCAGTCGTCGCAGTGTCATTTACTCAGGTCACAGCATCGAAAAGAGGTGAATAGCAGCAGCATAAAAGACCATCACATCCTGAGTGGATGGTGATTAGACTTAATTCTATTAAAGAGTTCAAGTTTCTCTTAGATCCTACCCAAGAAATGTTCTCCCCTCAACATTACAATCTCTCCTTTTTCTTTCTTGTGAGAATCTAGAATTCTTACCTCATGAATCCTTACCATATTGCACTTCACTTGAGAAATTGAAAATATTCAATATCTGTGATTCATTGACATCCTCTCGGCTAGTTCTCTCCTAGTCTTCAAAAGTCTTTTCATCTCAGGTTGTAAAAATCTAAAATCTTTTTCTTTTTCAGAAGAAGCGTCCCATAGTCATCCCTTTCTTCAAACTTTATCCATATATGCTTGTCCCAATCTTCGGTCATTTCCACTTCATAGACTGAATGCTCACCAATTCAGAGTCTGTCTAGCCTTTATCAATTGAGTGTGTATGGACGTCGAAAACTGCAGGCTTTTGCTCAAGAAAATTTACAATGAATTTAAGGATACTTGAAGTGTCCTATTTTGAGAGTTTGTCTACTTCAGCTATCACTAGGCGGGGTTTGCATTCCCTTGTCTTACAGCGTTGTGAATTCGAGACGATGGTCGTATGAATTCCTTGATGAAAATTGGAGAGTCATTGCTTCCGACTTCCCTCATATAGTCGGAAGCAATGACTCCAATTTTCATCTTTATTATTTAAAATGCTTAAAAGGGAAGTTGCTTCAGCATCTCACATCTCTTGAAAACCTTGAGATTTCAGATTGTCGGCGGCTTGAATCCTTACCAGAAGAAGGACTGCCTTCCTCTCTTTCAGTAGTGACTATCAATGATGCTTTTACTACAACCAAGCTGTCAGAGCAAGGAGGGAACGAGTGGCATAAGATTGCACATAATCCATGCATAATTACTGATAACAAAGTGATAATCTGA
Protein sequence

>MS.gene046218.t1

MNGSTIPIVEGVEKLVNNIDVNIVQNGGESVVDYTPHLEMEFESEAAAYEFYNKYSRRIGFGIRREYGNKSKKDGILTSRRFTCFKEGKRGVDKRDHLTKEGRVETRTGCDARMVISLDRKIGKYKVVDFVAQHNHFLEPPGHFHTPRSHRQISESRLSQVVAADESGLKRKDFHEYVYKQDGGIDAVGYLRTKRMRSLMYGEVGALLMHFKRQSENPSFFYDFQMDVEEQITNVFWADAQMINDYGYFGDVITFDTTYMTNKDYRPLGVFVGLNNHKQMVVFGATLLYDETIPSFQWLFETFLKAMGGEKPKTLLTDQHATMAKAISLVMPETFHGLCTWRMRENAQRHVNHLYQESSKFCSDFEACIDLHEEEGVFLNAWNVLLVEHNVSEDSWLRMIFQLKEKWAWAYVRKHFTAGMRSTQISESFNAELKNYLKSDLNLVQFFTHLERVVHGIRNNESEADYESRHELPKLKMKRAPMLVQAGNIYTPKTFEEFQEEYEEYLGTCVKNLKEGLYAVTNYDNNKERTVMGNLMDQKVVCDCRKFETHGILCSHALKVLDVMNIKLIPQHYILKRWTRDARLGSNQDLEGQPIELDIKAHFMRRYNELCPQMVKLINKASKSHETYTFLSKVFEESNKIIDDMLAKKFVGAESLELCHVSIPIGTEKSDNNVNTLDVGGEQEEASHSHPFLQTLSIYACPNLRSFPLHRLNAHQFRLSLGGVCIPLSYSVVNSRRWSYEFLDENWRVIASDFPHIVGSNDSNFHLYYLKCLKGKLLQHLTSLENLEISDCRRLESLPEEGLPSSLSVVTINDAFTTTKLSEQGGNEWHKIAHNPCIITDNKVII