Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04622.t1 | XP_013466755.2 | 57 | 165 | 39 | 2 | 36 | 168 | 645 | 809 | 7.00E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04622.t1 | A0A396JLZ7 | 66.7 | 105 | 35 | 0 | 42 | 146 | 4 | 108 | 1.5e-34 | 155.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04622.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04622.t1 | MTR_1g035640 | 51.020 | 98 | 48 | 0 | 42 | 139 | 165 | 262 | 4.52e-26 | 103 |
| MS.gene04622.t1 | MTR_1g050845 | 52.174 | 92 | 40 | 3 | 52 | 139 | 158 | 249 | 2.52e-19 | 84.7 |
| MS.gene04622.t1 | MTR_1g035570 | 43.333 | 90 | 51 | 0 | 50 | 139 | 180 | 269 | 4.72e-19 | 83.2 |
| MS.gene04622.t1 | MTR_1g035620 | 49.020 | 102 | 45 | 4 | 42 | 139 | 163 | 261 | 7.94e-19 | 82.8 |
| MS.gene04622.t1 | MTR_1g035490 | 46.341 | 82 | 44 | 0 | 58 | 139 | 97 | 178 | 2.37e-17 | 78.2 |
| MS.gene04622.t1 | MTR_6g061070 | 43.750 | 112 | 54 | 4 | 37 | 139 | 193 | 304 | 1.50e-16 | 76.3 |
| MS.gene04622.t1 | MTR_5g044670 | 36.957 | 92 | 55 | 2 | 58 | 148 | 205 | 294 | 8.22e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTATTGAACATCTCTT+AGG | 0.278157 | 7.3:+17464433 | None:intergenic |
| GGGACCCCTTCTACAAATTC+AGG | 0.347036 | 7.3:-17463838 | MS.gene04622:CDS |
| GAACCTCCTGAATTTGTAGA+AGG | 0.363430 | 7.3:+17463832 | None:intergenic |
| TAGCATCACTGCCACGGCCT+TGG | 0.370846 | 7.3:+17465654 | None:intergenic |
| TTGACGGGATCGCAAGGATA+AGG | 0.384450 | 7.3:-17464214 | MS.gene04622:CDS |
| ACTCACGAAGTTGAATAGTT+TGG | 0.408216 | 7.3:+17465615 | None:intergenic |
| TGACGGGATCGCAAGGATAA+GGG | 0.470585 | 7.3:-17464213 | MS.gene04622:CDS |
| AATGTCAGTGTCTAGTTGAA+TGG | 0.484492 | 7.3:+17465697 | None:intergenic |
| AGGCCGTGGCAGTGATGCTA+AGG | 0.488026 | 7.3:-17465651 | MS.gene04622:CDS |
| AATACTTGAACGAGTTTGAC+GGG | 0.492831 | 7.3:-17464229 | MS.gene04622:CDS |
| GCCACGGCCTTGGTGAATGC+AGG | 0.515591 | 7.3:+17465664 | None:intergenic |
| AAGGAAAATAACTTGCAAGT+TGG | 0.532958 | 7.3:-17464101 | MS.gene04622:CDS |
| GGGTTGATGCAGGTAGAACT+TGG | 0.542033 | 7.3:-17464193 | MS.gene04622:CDS |
| GAATACTTGAACGAGTTTGA+CGG | 0.544212 | 7.3:-17464230 | MS.gene04622:CDS |
| ACCTGCATTCACCAAGGCCG+TGG | 0.545742 | 7.3:-17465665 | MS.gene04622:CDS |
| AACCTCCTGAATTTGTAGAA+GGG | 0.561604 | 7.3:+17463833 | None:intergenic |
| ACCTCCTGAATTTGTAGAAG+GGG | 0.563047 | 7.3:+17463834 | None:intergenic |
| ACGAGTTTGACGGGATCGCA+AGG | 0.567127 | 7.3:-17464220 | MS.gene04622:CDS |
| GCAAGGATAAGGGTTGATGC+AGG | 0.581356 | 7.3:-17464203 | MS.gene04622:CDS |
| AACTTTGCAGGTACTAACAA+AGG | 0.591292 | 7.3:-17464466 | MS.gene04622:intron |
| GAACTTGGAAAGTGAATGTG+AGG | 0.592866 | 7.3:-17464178 | MS.gene04622:CDS |
| AATTAATCATACCGCGTACG+AGG | 0.595783 | 7.3:+17463768 | None:intergenic |
| GCAGGTACTAACAAAGGAGG+AGG | 0.600379 | 7.3:-17464460 | MS.gene04622:intron |
| TTCCCTTAGCATCACTGCCA+CGG | 0.603534 | 7.3:+17465648 | None:intergenic |
| TGGTTGGTCTTTATTCAACA+AGG | 0.607895 | 7.3:-17464120 | MS.gene04622:CDS |
| ATGACACAAACTTATGCCCA+TGG | 0.615053 | 7.3:-17464361 | MS.gene04622:CDS |
| ACCCCTTCTACAAATTCAGG+AGG | 0.639356 | 7.3:-17463835 | MS.gene04622:CDS |
| GGCCGTGGCAGTGATGCTAA+GGG | 0.651042 | 7.3:-17465650 | MS.gene04622:CDS |
| TTTGCAGGTACTAACAAAGG+AGG | 0.671084 | 7.3:-17464463 | MS.gene04622:intron |
| CAACATACCTGCATTCACCA+AGG | 0.683546 | 7.3:-17465671 | MS.gene04622:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATAATCAATAGTAAT+TGG | + | chr7.3:17464868-17464887 | None:intergenic | 10.0% |
| !!! | ATTGTTTTTATAATAATGAT+CGG | - | chr7.3:17464140-17464159 | MS.gene04622:CDS | 10.0% |
| !! | ACAAATCAAAAATATACAAA+TGG | + | chr7.3:17464326-17464345 | None:intergenic | 15.0% |
| !! | ATAGAAATATTAAATGAAAG+CGG | + | chr7.3:17465434-17465453 | None:intergenic | 15.0% |
| !!! | ATTTGTATATTTTTGATTTG+TGG | - | chr7.3:17464325-17464344 | MS.gene04622:intron | 15.0% |
| !!! | TATTCTTTTGAAAACTTATT+TGG | + | chr7.3:17465230-17465249 | None:intergenic | 15.0% |
| !!! | TTGAAAATGTTTACTAATTT+TGG | - | chr7.3:17465159-17465178 | MS.gene04622:intron | 15.0% |
| !! | AAACAATAAAATGTTCATTC+AGG | + | chr7.3:17464285-17464304 | None:intergenic | 20.0% |
| !! | AATCAAAAATATACAAATGG+AGG | + | chr7.3:17464323-17464342 | None:intergenic | 20.0% |
| !! | AGTGATAAAAGGAATTAAAA+AGG | + | chr7.3:17465600-17465619 | None:intergenic | 20.0% |
| !!! | AGTCTAAGATGATTTAAAAA+TGG | + | chr7.3:17464527-17464546 | None:intergenic | 20.0% |
| !!! | ATAATGAGTCTTGTTTATTA+AGG | + | chr7.3:17463991-17464010 | None:intergenic | 20.0% |
| !!! | CTGTATATTGATTTTGTATT+TGG | - | chr7.3:17464600-17464619 | MS.gene04622:intron | 20.0% |
| ! | AAAACCAATGAGTTTAAACT+TGG | + | chr7.3:17464670-17464689 | None:intergenic | 25.0% |
| ! | AAAGCAAACTTACACAAAAA+TGG | + | chr7.3:17465140-17465159 | None:intergenic | 25.0% |
| ! | AACTTTCACAATTGTAAGTT+TGG | - | chr7.3:17464460-17464479 | MS.gene04622:intron | 25.0% |
| ! | ACTGACTAGGTTATAAAAAA+GGG | - | chr7.3:17465617-17465636 | MS.gene04622:intron | 25.0% |
| ! | AGAATATACATTAACTTTGC+AGG | - | chr7.3:17464997-17465016 | MS.gene04622:intron | 25.0% |
| ! | ATTGAACAAGTAATGAACTT+TGG | + | chr7.3:17464647-17464666 | None:intergenic | 25.0% |
| ! | GACTTATCATGTTAGTAATT+TGG | - | chr7.3:17464543-17464562 | MS.gene04622:intron | 25.0% |
| ! | GATGATTTAAAAATGGACAA+TGG | + | chr7.3:17464520-17464539 | None:intergenic | 25.0% |
| ! | GTATATTCTAATCATTTCCA+AGG | + | chr7.3:17464986-17465005 | None:intergenic | 25.0% |
| ! | TATCTCTCTACAAAATTCTT+TGG | + | chr7.3:17464716-17464735 | None:intergenic | 25.0% |
| ! | TCAATTATTGAACATCTCTT+AGG | + | chr7.3:17465045-17465064 | None:intergenic | 25.0% |
| ! | TTAAAAATGGACAATGGTTT+AGG | + | chr7.3:17464514-17464533 | None:intergenic | 25.0% |
| !! | AAAACTTGGATTCTCATTTT+TGG | + | chr7.3:17465085-17465104 | None:intergenic | 25.0% |
| !! | AATGGTTTAGGTTACAAAAA+TGG | + | chr7.3:17464502-17464521 | None:intergenic | 25.0% |
| !! | AGAATTTTGTAGAGAGATAT+TGG | - | chr7.3:17464717-17464736 | MS.gene04622:intron | 25.0% |
| !!! | GTCATTTTAAGTTCAAAACT+TGG | + | chr7.3:17465099-17465118 | None:intergenic | 25.0% |
| !!! | TTTTGTATTTGGTTTGAGTT+TGG | - | chr7.3:17464611-17464630 | MS.gene04622:intron | 25.0% |
| AAGAGAAACTAATGAAGTAG+AGG | + | chr7.3:17464844-17464863 | None:intergenic | 30.0% | |
| AATATTAAGTAGTCGCATAG+TGG | - | chr7.3:17464167-17464186 | MS.gene04622:CDS | 30.0% | |
| ATAACCTAGTCAGTGATAAA+AGG | + | chr7.3:17465611-17465630 | None:intergenic | 30.0% | |
| ATGATATATCATAGCTTGAG+TGG | + | chr7.3:17464072-17464091 | None:intergenic | 30.0% | |
| CAATCCAAGTTTAAACTCAT+TGG | - | chr7.3:17464663-17464682 | MS.gene04622:intron | 30.0% | |
| TATACCATGAATTGAAACAG+AGG | - | chr7.3:17465201-17465220 | MS.gene04622:intron | 30.0% | |
| TGATATATCATAGCTTGAGT+GGG | + | chr7.3:17464071-17464090 | None:intergenic | 30.0% | |
| ! | AACATCTCTAGTCGAATTTT+AGG | - | chr7.3:17464218-17464237 | MS.gene04622:CDS | 30.0% |
| ! | AAGGAAAATAACTTGCAAGT+TGG | - | chr7.3:17465374-17465393 | MS.gene04622:intron | 30.0% |
| ! | ACATCTCTAGTCGAATTTTA+GGG | - | chr7.3:17464219-17464238 | MS.gene04622:CDS | 30.0% |
| ! | CACTGACTAGGTTATAAAAA+AGG | - | chr7.3:17465616-17465635 | MS.gene04622:intron | 30.0% |
| !! | AAAAACCTTGCAACTTGTTA+GGG | + | chr7.3:17465481-17465500 | None:intergenic | 30.0% |
| !! | AATTCCTTTTATCACTGACT+AGG | - | chr7.3:17465604-17465623 | MS.gene04622:intron | 30.0% |
| !! | GTAATTTGGTTAGCTCTTTT+CGG | - | chr7.3:17464557-17464576 | MS.gene04622:intron | 30.0% |
| !!! | ACTTGCCTAAGACTTTTTTA+TGG | + | chr7.3:17464820-17464839 | None:intergenic | 30.0% |
| !!! | AGATGTTCTTTTTTGCAAGT+TGG | - | chr7.3:17465335-17465354 | MS.gene04622:intron | 30.0% |
| !!! | CTTGCCTAAGACTTTTTTAT+GGG | + | chr7.3:17464819-17464838 | None:intergenic | 30.0% |
| AACCTCCTGAATTTGTAGAA+GGG | + | chr7.3:17465645-17465664 | None:intergenic | 35.0% | |
| AACTTACACAAAAATGGCCA+TGG | + | chr7.3:17465134-17465153 | None:intergenic | 35.0% | |
| AACTTTGCAGGTACTAACAA+AGG | - | chr7.3:17465009-17465028 | MS.gene04622:intron | 35.0% | |
| AATACTTGAACGAGTTTGAC+GGG | - | chr7.3:17465246-17465265 | MS.gene04622:intron | 35.0% | |
| AATGTCAGTGTCTAGTTGAA+TGG | + | chr7.3:17463781-17463800 | None:intergenic | 35.0% | |
| ACTCACGAAGTTGAATAGTT+TGG | + | chr7.3:17463863-17463882 | None:intergenic | 35.0% | |
| ACTTACACAAAAATGGCCAT+GGG | + | chr7.3:17465133-17465152 | None:intergenic | 35.0% | |
| AGTTGTCAGGGTTGAAATAA+TGG | + | chr7.3:17464046-17464065 | None:intergenic | 35.0% | |
| ATAGTGTTCTGGTGTGATAA+AGG | + | chr7.3:17464384-17464403 | None:intergenic | 35.0% | |
| GAATACTTGAACGAGTTTGA+CGG | - | chr7.3:17465245-17465264 | MS.gene04622:intron | 35.0% | |
| GCAACCCATAAAAAAGTCTT+AGG | - | chr7.3:17464812-17464831 | MS.gene04622:intron | 35.0% | |
| GTATCCTCTGTTTCAATTCA+TGG | + | chr7.3:17465208-17465227 | None:intergenic | 35.0% | |
| GTGAACATGTAATAGTGTTC+TGG | + | chr7.3:17464395-17464414 | None:intergenic | 35.0% | |
| GTTGTCAGGGTTGAAATAAT+GGG | + | chr7.3:17464045-17464064 | None:intergenic | 35.0% | |
| TGTGAAAGTTTGTAAAAGCG+TGG | + | chr7.3:17464450-17464469 | None:intergenic | 35.0% | |
| ! | CATCTCTAGTCGAATTTTAG+GGG | - | chr7.3:17464220-17464239 | MS.gene04622:CDS | 35.0% |
| ! | GAAAAACCTTGCAACTTGTT+AGG | + | chr7.3:17465482-17465501 | None:intergenic | 35.0% |
| ! | GTGAAAGTTTGTAAAAGCGT+GGG | + | chr7.3:17464449-17464468 | None:intergenic | 35.0% |
| !! | TGGTTGGTCTTTATTCAACA+AGG | - | chr7.3:17465355-17465374 | MS.gene04622:intron | 35.0% |
| !!! | GTTCTTTTTTGCAAGTTGGT+TGG | - | chr7.3:17465339-17465358 | MS.gene04622:intron | 35.0% |
| ACCTCCTGAATTTGTAGAAG+GGG | + | chr7.3:17465644-17465663 | None:intergenic | 40.0% | |
| ATGACACAAACTTATGCCCA+TGG | - | chr7.3:17465114-17465133 | MS.gene04622:intron | 40.0% | |
| CAATATCCCTTAGACCTCAA+GGG | - | chr7.3:17464756-17464775 | MS.gene04622:intron | 40.0% | |
| GAACCTCCTGAATTTGTAGA+AGG | + | chr7.3:17465646-17465665 | None:intergenic | 40.0% | |
| GAACTTGGAAAGTGAATGTG+AGG | - | chr7.3:17465297-17465316 | MS.gene04622:intron | 40.0% | |
| TCAATATACAGATCCCTTGC+CGG | + | chr7.3:17464591-17464610 | None:intergenic | 40.0% | |
| TTGTCAATGTTTGTCCCTTG+AGG | + | chr7.3:17464773-17464792 | None:intergenic | 40.0% | |
| TTTGCAGGTACTAACAAAGG+AGG | - | chr7.3:17465012-17465031 | MS.gene04622:intron | 40.0% | |
| ! | TTTTGTGAATTCCTCGTACG+CGG | - | chr7.3:17465696-17465715 | MS.gene04622:CDS | 40.0% |
| ACCCCTTCTACAAATTCAGG+AGG | - | chr7.3:17465640-17465659 | MS.gene04622:CDS | 45.0% | |
| CAACATACCTGCATTCACCA+AGG | - | chr7.3:17463804-17463823 | MS.gene04622:CDS | 45.0% | |
| CCAATATCCCTTAGACCTCA+AGG | - | chr7.3:17464755-17464774 | MS.gene04622:intron | 45.0% | |
| CCTTGAGGTCTAAGGGATAT+TGG | + | chr7.3:17464758-17464777 | None:intergenic | 45.0% | |
| GTTTGTCCCTTGAGGTCTAA+GGG | + | chr7.3:17464765-17464784 | None:intergenic | 45.0% | |
| TGTTTGTCCCTTGAGGTCTA+AGG | + | chr7.3:17464766-17464785 | None:intergenic | 45.0% | |
| TTCTATTACCCGAGCTAGTG+AGG | - | chr7.3:17465448-17465467 | MS.gene04622:intron | 45.0% | |
| ! | GTTTGTAAAAGCGTGGGAGT+CGG | + | chr7.3:17464443-17464462 | None:intergenic | 45.0% |
| CTAAAGCTAACTGTGCGCCT+TGG | - | chr7.3:17464966-17464985 | MS.gene04622:intron | 50.0% | |
| GCAGGTACTAACAAAGGAGG+AGG | - | chr7.3:17465015-17465034 | MS.gene04622:intron | 50.0% | |
| GGGACCCCTTCTACAAATTC+AGG | - | chr7.3:17465637-17465656 | MS.gene04622:CDS | 50.0% | |
| TGACGGGATCGCAAGGATAA+GGG | - | chr7.3:17465262-17465281 | MS.gene04622:intron | 50.0% | |
| TTCCCTTAGCATCACTGCCA+CGG | + | chr7.3:17463830-17463849 | None:intergenic | 50.0% | |
| TTGACGGGATCGCAAGGATA+AGG | - | chr7.3:17465261-17465280 | MS.gene04622:intron | 50.0% | |
| ! | TGAGCGTGCTTACTAGCTCT+TGG | + | chr7.3:17463893-17463912 | None:intergenic | 50.0% |
| !! | CCTTGCAACTTGTTAGGGCT+CGG | + | chr7.3:17465476-17465495 | None:intergenic | 50.0% |
| !! | CTTGAGTGGGCTAGTTGTCA+GGG | + | chr7.3:17464058-17464077 | None:intergenic | 50.0% |
| !! | GCAAGGATAAGGGTTGATGC+AGG | - | chr7.3:17465272-17465291 | MS.gene04622:intron | 50.0% |
| !! | GGGTTGATGCAGGTAGAACT+TGG | - | chr7.3:17465282-17465301 | MS.gene04622:intron | 50.0% |
| ACGAGTTTGACGGGATCGCA+AGG | - | chr7.3:17465255-17465274 | MS.gene04622:intron | 55.0% | |
| ATACAGATCCCTTGCCGGTG+AGG | + | chr7.3:17464586-17464605 | None:intergenic | 55.0% | |
| CCGAGCCCTAACAAGTTGCA+AGG | - | chr7.3:17465473-17465492 | MS.gene04622:intron | 55.0% | |
| !! | GCTTGAGTGGGCTAGTTGTC+AGG | + | chr7.3:17464059-17464078 | None:intergenic | 55.0% |
| ACCTGCATTCACCAAGGCCG+TGG | - | chr7.3:17463810-17463829 | MS.gene04622:CDS | 60.0% | |
| AGGCCGTGGCAGTGATGCTA+AGG | - | chr7.3:17463824-17463843 | MS.gene04622:CDS | 60.0% | |
| GCTCGGTTCCTCACTAGCTC+GGG | + | chr7.3:17465459-17465478 | None:intergenic | 60.0% | |
| GGCCGTGGCAGTGATGCTAA+GGG | - | chr7.3:17463825-17463844 | MS.gene04622:CDS | 60.0% | |
| TAGCATCACTGCCACGGCCT+TGG | + | chr7.3:17463824-17463843 | None:intergenic | 60.0% | |
| ! | GCTCTTTTCGGCGTCCTCAC+CGG | - | chr7.3:17464569-17464588 | MS.gene04622:intron | 60.0% |
| ! | GGCTCGGTTCCTCACTAGCT+CGG | + | chr7.3:17465460-17465479 | None:intergenic | 60.0% |
| GCCACGGCCTTGGTGAATGC+AGG | + | chr7.3:17463814-17463833 | None:intergenic | 65.0% | |
| TTCGGCGTCCTCACCGGCAA+GGG | - | chr7.3:17464575-17464594 | MS.gene04622:intron | 65.0% | |
| TTTCGGCGTCCTCACCGGCA+AGG | - | chr7.3:17464574-17464593 | MS.gene04622:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 17463774 | 17465723 | 17463774 | ID=MS.gene04622 |
| chr7.3 | mRNA | 17463774 | 17465723 | 17463774 | ID=MS.gene04622.t1;Parent=MS.gene04622 |
| chr7.3 | exon | 17465621 | 17465723 | 17465621 | ID=MS.gene04622.t1.exon1;Parent=MS.gene04622.t1 |
| chr7.3 | CDS | 17465621 | 17465723 | 17465621 | ID=cds.MS.gene04622.t1;Parent=MS.gene04622.t1 |
| chr7.3 | exon | 17464351 | 17464478 | 17464351 | ID=MS.gene04622.t1.exon2;Parent=MS.gene04622.t1 |
| chr7.3 | CDS | 17464351 | 17464478 | 17464351 | ID=cds.MS.gene04622.t1;Parent=MS.gene04622.t1 |
| chr7.3 | exon | 17464093 | 17464276 | 17464093 | ID=MS.gene04622.t1.exon3;Parent=MS.gene04622.t1 |
| chr7.3 | CDS | 17464093 | 17464276 | 17464093 | ID=cds.MS.gene04622.t1;Parent=MS.gene04622.t1 |
| chr7.3 | exon | 17463774 | 17463871 | 17463774 | ID=MS.gene04622.t1.exon4;Parent=MS.gene04622.t1 |
| chr7.3 | CDS | 17463774 | 17463871 | 17463774 | ID=cds.MS.gene04622.t1;Parent=MS.gene04622.t1 |
| Gene Sequence |
| Protein sequence |
