Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046276.t1 | PNX75333.1 | 98.3 | 631 | 11 | 0 | 47 | 677 | 1 | 631 | 0.00E+00 | 1260 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046276.t1 | Q9SZL8 | 37.8 | 588 | 349 | 5 | 24 | 596 | 54 | 639 | 1.1e-124 | 448.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046276.t1 | A0A2K3L9Y4 | 98.3 | 631 | 11 | 0 | 47 | 677 | 1 | 631 | 0.0e+00 | 1260.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046276.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046276.t1 | MTR_1g019850 | 94.737 | 171 | 9 | 0 | 1 | 171 | 28 | 198 | 1.87e-111 | 335 |
| MS.gene046276.t1 | MTR_0734s0010 | 95.833 | 120 | 5 | 0 | 558 | 677 | 1 | 120 | 8.51e-77 | 244 |
| MS.gene046276.t1 | MTR_0853s0010 | 56.774 | 155 | 52 | 2 | 30 | 169 | 4 | 158 | 6.09e-51 | 174 |
| MS.gene046276.t1 | MTR_2g024230 | 79.000 | 100 | 20 | 1 | 1 | 99 | 47 | 146 | 7.03e-46 | 160 |
| MS.gene046276.t1 | MTR_6g051425 | 34.498 | 229 | 137 | 4 | 82 | 300 | 64 | 289 | 8.16e-38 | 144 |
| MS.gene046276.t1 | MTR_4g035820 | 26.087 | 299 | 193 | 8 | 6 | 277 | 27 | 324 | 4.91e-23 | 104 |
| MS.gene046276.t1 | MTR_4g035820 | 37.755 | 98 | 61 | 0 | 411 | 508 | 327 | 424 | 1.71e-11 | 67.8 |
| MS.gene046276.t1 | MTR_0558s0020 | 25.292 | 257 | 162 | 5 | 47 | 273 | 40 | 296 | 6.70e-22 | 97.1 |
| MS.gene046276.t1 | MTR_3g450850 | 28.819 | 288 | 168 | 11 | 3 | 261 | 7 | 286 | 3.81e-20 | 92.8 |
| MS.gene046276.t1 | MTR_3g020080 | 47.475 | 99 | 31 | 2 | 383 | 477 | 3 | 84 | 4.19e-20 | 87.4 |
| MS.gene046276.t1 | MTR_8g446020 | 27.717 | 184 | 116 | 2 | 101 | 273 | 79 | 256 | 4.56e-19 | 87.8 |
| MS.gene046276.t1 | MTR_1g070355 | 25.322 | 233 | 159 | 5 | 48 | 267 | 79 | 309 | 5.86e-18 | 88.2 |
| MS.gene046276.t1 | MTR_5g076070 | 27.059 | 255 | 165 | 8 | 21 | 261 | 35 | 282 | 2.72e-17 | 83.2 |
| MS.gene046276.t1 | MTR_6g022070 | 30.233 | 172 | 114 | 2 | 327 | 497 | 1 | 167 | 1.64e-16 | 81.6 |
| MS.gene046276.t1 | MTR_6g090505 | 25.773 | 194 | 139 | 2 | 101 | 289 | 54 | 247 | 3.22e-16 | 81.3 |
| MS.gene046276.t1 | MTR_1g033820 | 50.704 | 71 | 29 | 1 | 23 | 87 | 1 | 71 | 1.60e-15 | 72.8 |
| MS.gene046276.t1 | MTR_7g446480 | 28.409 | 176 | 119 | 3 | 112 | 281 | 140 | 314 | 2.78e-15 | 77.8 |
| MS.gene046276.t1 | MTR_7g446510 | 26.857 | 175 | 123 | 1 | 112 | 281 | 155 | 329 | 5.96e-15 | 77.0 |
| MS.gene046276.t1 | MTR_6g048540 | 40.000 | 110 | 61 | 2 | 566 | 672 | 106 | 213 | 7.61e-15 | 75.1 |
| MS.gene046276.t1 | MTR_6g048540 | 52.542 | 59 | 28 | 0 | 38 | 96 | 23 | 81 | 3.49e-12 | 67.0 |
| MS.gene046276.t1 | MTR_1g083640 | 22.344 | 273 | 181 | 6 | 6 | 248 | 15 | 286 | 2.22e-13 | 71.6 |
| MS.gene046276.t1 | MTR_1g013060 | 25.439 | 228 | 147 | 6 | 23 | 234 | 10 | 230 | 6.54e-13 | 69.3 |
| MS.gene046276.t1 | MTR_8g466860 | 73.684 | 38 | 10 | 0 | 398 | 435 | 81 | 118 | 2.75e-12 | 64.7 |
| MS.gene046276.t1 | MTR_7g446520 | 25.000 | 244 | 154 | 8 | 44 | 275 | 89 | 315 | 3.09e-12 | 68.9 |
| MS.gene046276.t1 | MTR_8g465780 | 31.068 | 103 | 70 | 1 | 404 | 505 | 1 | 103 | 4.26e-12 | 65.9 |
| MS.gene046276.t1 | MTR_7g074790 | 30.058 | 173 | 85 | 6 | 43 | 201 | 10 | 160 | 3.37e-11 | 64.3 |
| MS.gene046276.t1 | MTR_5g039760 | 25.397 | 189 | 114 | 5 | 45 | 226 | 12 | 180 | 3.51e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046276.t1 | AT4G38180 | 37.755 | 588 | 349 | 5 | 24 | 596 | 54 | 639 | 2.61e-151 | 458 |
| MS.gene046276.t1 | AT2G27110 | 32.845 | 682 | 407 | 15 | 1 | 665 | 1 | 648 | 5.64e-122 | 384 |
| MS.gene046276.t1 | AT2G27110 | 32.845 | 682 | 407 | 15 | 1 | 665 | 1 | 648 | 5.64e-122 | 384 |
| MS.gene046276.t1 | AT2G27110 | 36.876 | 461 | 282 | 4 | 206 | 665 | 51 | 503 | 4.49e-112 | 353 |
| MS.gene046276.t1 | AT4G15090 | 31.250 | 656 | 424 | 8 | 36 | 676 | 44 | 687 | 8.70e-112 | 356 |
| MS.gene046276.t1 | AT4G15090 | 31.250 | 656 | 424 | 8 | 36 | 676 | 44 | 687 | 8.70e-112 | 356 |
| MS.gene046276.t1 | AT4G15090 | 31.250 | 656 | 424 | 8 | 36 | 676 | 44 | 687 | 8.70e-112 | 356 |
| MS.gene046276.t1 | AT4G15090 | 31.250 | 656 | 424 | 8 | 36 | 676 | 44 | 687 | 8.70e-112 | 356 |
| MS.gene046276.t1 | AT4G15090 | 31.250 | 656 | 424 | 8 | 36 | 676 | 44 | 687 | 8.70e-112 | 356 |
| MS.gene046276.t1 | AT4G38170 | 38.600 | 443 | 260 | 7 | 210 | 648 | 21 | 455 | 4.58e-108 | 338 |
| MS.gene046276.t1 | AT3G22170 | 32.079 | 611 | 369 | 10 | 1 | 590 | 32 | 617 | 1.12e-107 | 345 |
| MS.gene046276.t1 | AT3G22170 | 32.079 | 611 | 369 | 10 | 1 | 590 | 32 | 617 | 1.12e-107 | 345 |
| MS.gene046276.t1 | AT1G76320 | 33.938 | 551 | 349 | 6 | 47 | 590 | 1 | 543 | 1.10e-102 | 328 |
| MS.gene046276.t1 | AT1G76320 | 33.938 | 551 | 349 | 6 | 47 | 590 | 1 | 543 | 3.09e-102 | 328 |
| MS.gene046276.t1 | AT1G76320 | 33.938 | 551 | 349 | 6 | 47 | 590 | 1 | 543 | 3.96e-102 | 328 |
| MS.gene046276.t1 | AT1G76320 | 33.938 | 551 | 349 | 6 | 47 | 590 | 1 | 543 | 3.96e-102 | 328 |
| MS.gene046276.t1 | AT3G06250 | 30.511 | 626 | 384 | 10 | 30 | 648 | 178 | 759 | 2.48e-97 | 316 |
| MS.gene046276.t1 | AT3G06250 | 30.511 | 626 | 384 | 10 | 30 | 648 | 178 | 759 | 2.48e-97 | 316 |
| MS.gene046276.t1 | AT3G06250 | 30.511 | 626 | 384 | 10 | 30 | 648 | 178 | 759 | 2.48e-97 | 316 |
| MS.gene046276.t1 | AT5G18960 | 31.433 | 614 | 372 | 12 | 43 | 648 | 211 | 783 | 4.65e-95 | 311 |
| MS.gene046276.t1 | AT5G18960 | 31.433 | 614 | 372 | 12 | 43 | 648 | 211 | 783 | 4.65e-95 | 311 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 4.45e-94 | 308 |
| MS.gene046276.t1 | AT2G32250 | 30.293 | 614 | 377 | 9 | 47 | 653 | 1 | 570 | 1.54e-93 | 306 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 1.62e-93 | 307 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 1.62e-93 | 307 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 1.69e-93 | 307 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 1.69e-93 | 307 |
| MS.gene046276.t1 | AT2G32250 | 29.618 | 655 | 402 | 11 | 14 | 653 | 1 | 611 | 1.69e-93 | 307 |
| MS.gene046276.t1 | AT2G32250 | 30.293 | 614 | 377 | 9 | 47 | 653 | 1 | 570 | 2.05e-93 | 306 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 26 | 574 | 1.99e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 26 | 574 | 1.99e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 26 | 574 | 2.66e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 26 | 574 | 3.63e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 26 | 574 | 3.63e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 30.703 | 583 | 346 | 7 | 48 | 606 | 40 | 588 | 3.67e-89 | 295 |
| MS.gene046276.t1 | AT4G19990 | 32.617 | 512 | 307 | 5 | 99 | 606 | 40 | 517 | 6.07e-87 | 287 |
| MS.gene046276.t1 | AT1G52520 | 28.549 | 634 | 419 | 13 | 43 | 657 | 85 | 703 | 3.71e-86 | 285 |
| MS.gene046276.t1 | AT1G10240 | 29.362 | 596 | 380 | 14 | 20 | 588 | 23 | 604 | 1.61e-82 | 275 |
| MS.gene046276.t1 | AT1G10240 | 29.362 | 596 | 380 | 14 | 20 | 588 | 23 | 604 | 1.61e-82 | 275 |
| MS.gene046276.t1 | AT1G80010 | 30.293 | 581 | 361 | 14 | 43 | 592 | 95 | 662 | 1.41e-81 | 273 |
| MS.gene046276.t1 | AT5G28530 | 26.117 | 582 | 396 | 10 | 43 | 600 | 55 | 626 | 2.87e-64 | 226 |
| MS.gene046276.t1 | AT5G28530 | 26.117 | 582 | 396 | 10 | 43 | 600 | 55 | 626 | 2.87e-64 | 226 |
| MS.gene046276.t1 | AT5G28530 | 25.945 | 582 | 401 | 10 | 43 | 600 | 55 | 630 | 5.19e-64 | 225 |
| MS.gene046276.t1 | AT5G28530 | 24.956 | 569 | 356 | 12 | 43 | 600 | 55 | 563 | 3.61e-46 | 174 |
| MS.gene046276.t1 | AT5G28530 | 24.780 | 569 | 361 | 12 | 43 | 600 | 55 | 567 | 8.99e-46 | 172 |
Find 92 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTTGCTACAATGTTTC+AGG | 0.177913 | 3.4:-8834372 | MS.gene046276:CDS |
| AACTCCAAATCCTTCTTTCT+TGG | 0.222792 | 3.4:+8834700 | None:intergenic |
| TATTTGTCCTATTGTTGATA+TGG | 0.229024 | 3.4:-8834768 | MS.gene046276:CDS |
| AAGGAGAATGAATGGCTTAA+AGG | 0.244725 | 3.4:-8833702 | MS.gene046276:CDS |
| CTCGTCACCGGTTATGTTTA+TGG | 0.247826 | 3.4:-8833875 | MS.gene046276:CDS |
| ATTCATAGTTTGTGTGGATT+TGG | 0.266789 | 3.4:-8833280 | MS.gene046276:CDS |
| ATAGAATGATAAATTTCTTC+TGG | 0.268818 | 3.4:-8834187 | MS.gene046276:CDS |
| TTATAAAGATTATTCAATTT+AGG | 0.268994 | 3.4:-8833374 | MS.gene046276:CDS |
| AAGCAGGGAAGAGAAGTAAA+TGG | 0.304863 | 3.4:-8834535 | MS.gene046276:CDS |
| CGCAGATAGGCCACACTCTT+AGG | 0.314469 | 3.4:+8834467 | None:intergenic |
| AATTTATGGGATTGCTATGT+AGG | 0.326606 | 3.4:-8833221 | MS.gene046276:CDS |
| TAAGCTGATATCATCTGATT+TGG | 0.331906 | 3.4:-8832983 | MS.gene046276:CDS |
| TCTTCAAAGTATTCTATAGA+TGG | 0.346727 | 3.4:+8833759 | None:intergenic |
| TTTGCCAAGAAAGAAGGATT+TGG | 0.365412 | 3.4:-8834704 | MS.gene046276:CDS |
| AGAAGAATGGGAACGTATAA+TGG | 0.393483 | 3.4:-8833739 | MS.gene046276:CDS |
| TTCTTCTGAAATTTCTAAGA+TGG | 0.394077 | 3.4:+8834312 | None:intergenic |
| AATGGTTGATTATAATTTGA+AGG | 0.401452 | 3.4:-8833721 | MS.gene046276:CDS |
| TAGAATACTTTGAAGAAGAA+TGG | 0.402134 | 3.4:-8833752 | MS.gene046276:CDS |
| CGCTTTAAAATATACATGTC+TGG | 0.419734 | 3.4:+8833156 | None:intergenic |
| AAATGGCATATCATACTTGT+TGG | 0.423697 | 3.4:+8834094 | None:intergenic |
| AGGAGAATGAATGGCTTAAA+GGG | 0.425174 | 3.4:-8833701 | MS.gene046276:CDS |
| TCAAATTCCATATCAACAAT+AGG | 0.427064 | 3.4:+8834761 | None:intergenic |
| ATAATTTGAAGGAGAATGAA+TGG | 0.429066 | 3.4:-8833710 | MS.gene046276:CDS |
| TAGAATGATAAATTTCTTCT+GGG | 0.433286 | 3.4:-8834186 | MS.gene046276:CDS |
| GGTGGATGGAAGAGCAAGAT+TGG | 0.436268 | 3.4:-8834165 | MS.gene046276:CDS |
| AAAGTGCTTCTGTTGAAAAC+AGG | 0.437656 | 3.4:+8833651 | None:intergenic |
| GAAGGTACACATATAGTAAA+GGG | 0.439216 | 3.4:-8834629 | MS.gene046276:CDS |
| TTATGTGCCATAAACATAAC+CGG | 0.445245 | 3.4:+8833868 | None:intergenic |
| TAAAGATTATTCAATTTAGG+AGG | 0.447983 | 3.4:-8833371 | MS.gene046276:CDS |
| TCACTTACTGTTTCAAAAGC+AGG | 0.452498 | 3.4:-8834551 | MS.gene046276:CDS |
| ATAAATTTCTTCTGGGTGGA+TGG | 0.455401 | 3.4:-8834179 | MS.gene046276:CDS |
| ATAAACAATGTTCATAATCA+TGG | 0.455589 | 3.4:-8834500 | MS.gene046276:CDS |
| AGAATACTTTGAAGAAGAAT+GGG | 0.456244 | 3.4:-8833751 | MS.gene046276:CDS |
| ACATCGTTTGCCAAGAAAGA+AGG | 0.463881 | 3.4:-8834710 | MS.gene046276:CDS |
| ATAGGACAAATATTAGCTTC+AGG | 0.470032 | 3.4:+8834779 | None:intergenic |
| GTTGAGAAGTTTGATGAAGA+AGG | 0.472036 | 3.4:-8834407 | MS.gene046276:CDS |
| GGACCAGAGTCTTCCTCTAC+AGG | 0.475137 | 3.4:-8833090 | MS.gene046276:CDS |
| AATCCGGTGAAGGGTGCAAA+TGG | 0.486093 | 3.4:+8834077 | None:intergenic |
| GCTGATATCATCTGATTTGG+AGG | 0.486826 | 3.4:-8832980 | MS.gene046276:CDS |
| AGGGTCTCAGTATAGATATA+AGG | 0.487060 | 3.4:-8833331 | MS.gene046276:CDS |
| AAGGATTTGGAGTTCGTGTT+CGG | 0.488165 | 3.4:-8834691 | MS.gene046276:CDS |
| AGCAGGGAAGAGAAGTAAAT+GGG | 0.489503 | 3.4:-8834534 | MS.gene046276:CDS |
| CATAACCGGTGACGAGTATG+TGG | 0.490741 | 3.4:+8833882 | None:intergenic |
| TGAAGGTACACATATAGTAA+AGG | 0.494494 | 3.4:-8834630 | MS.gene046276:CDS |
| GGTGCTGTGTTTATTGAAGA+TGG | 0.495192 | 3.4:-8833111 | MS.gene046276:CDS |
| AATATTAGCTTCAGGCGACT+TGG | 0.496239 | 3.4:+8834787 | None:intergenic |
| ATGCCATTTGCACCCTTCAC+CGG | 0.499873 | 3.4:-8834080 | MS.gene046276:CDS |
| GATGAAGAAGGAGTACCTAC+AGG | 0.503271 | 3.4:-8834395 | MS.gene046276:CDS |
| AAAGATTATTCAATTTAGGA+GGG | 0.507177 | 3.4:-8833370 | MS.gene046276:CDS |
| GTAAAGGGTGAAAATGAAGA+AGG | 0.507431 | 3.4:-8834614 | MS.gene046276:CDS |
| TTGAAAATGATGATTCAATC+CGG | 0.518170 | 3.4:+8834061 | None:intergenic |
| TTTCTTATGAGACCGCAGAT+AGG | 0.520274 | 3.4:+8834454 | None:intergenic |
| TGATCTGTTATTATTGACAC+TGG | 0.529855 | 3.4:+8833942 | None:intergenic |
| AAAGTTGCTACAATGTTTCA+GGG | 0.533512 | 3.4:-8834371 | MS.gene046276:CDS |
| AGTTAAATTTGACAAGGCAA+TGG | 0.535325 | 3.4:-8833535 | MS.gene046276:CDS |
| TGCTTTACAATCAGTTCTTG+AGG | 0.537334 | 3.4:+8834574 | None:intergenic |
| TGTACGATATAAGAAAATCA+TGG | 0.540635 | 3.4:-8833677 | MS.gene046276:CDS |
| TAGAACATGGCTAGACGCAA+TGG | 0.540817 | 3.4:-8833976 | MS.gene046276:CDS |
| CACTTACTGTTTCAAAAGCA+GGG | 0.542698 | 3.4:-8834550 | MS.gene046276:CDS |
| AGAAGAAGAAATCTTGATGT+GGG | 0.546130 | 3.4:-8834296 | MS.gene046276:CDS |
| ATGATGATTCAATCCGGTGA+AGG | 0.546228 | 3.4:+8834067 | None:intergenic |
| ATTGCTATGTAGGCATATCT+TGG | 0.547174 | 3.4:-8833211 | MS.gene046276:CDS |
| ATGTGGGAAAACATTGGCTA+TGG | 0.551030 | 3.4:+8833899 | None:intergenic |
| TAAAGGGTGAAAATGAAGAA+GGG | 0.554329 | 3.4:-8834613 | MS.gene046276:CDS |
| CAGAAGAAGAAATCTTGATG+TGG | 0.556138 | 3.4:-8834297 | MS.gene046276:CDS |
| AGACACATTCATAGTTTGTG+TGG | 0.560120 | 3.4:-8833286 | MS.gene046276:CDS |
| CAATGTTCATAATCATGGAA+TGG | 0.563943 | 3.4:-8834495 | MS.gene046276:CDS |
| AGAACATGGCTAGACGCAAT+GGG | 0.566082 | 3.4:-8833975 | MS.gene046276:CDS |
| ATTGTAGCAACTTTCCCTGT+AGG | 0.569258 | 3.4:+8834380 | None:intergenic |
| AGTTGATGAGTTTCCTGTAG+AGG | 0.572088 | 3.4:+8833077 | None:intergenic |
| TTGTGTGGATTTGGAGACAA+AGG | 0.575494 | 3.4:-8833271 | MS.gene046276:CDS |
| TTGAGTGGTTGTTTAGAACA+TGG | 0.580129 | 3.4:-8833989 | MS.gene046276:CDS |
| ACACATCATCAACTATCTCA+TGG | 0.582009 | 3.4:+8834840 | None:intergenic |
| CCTTTGTGTAGTGTTCATGC+CGG | 0.583834 | 3.4:+8833623 | None:intergenic |
| AATGATAAATTTCTTCTGGG+TGG | 0.588712 | 3.4:-8834183 | MS.gene046276:CDS |
| TGATGATTCAATCCGGTGAA+GGG | 0.589411 | 3.4:+8834068 | None:intergenic |
| ATGTTTGTATGCGCTAATGA+AGG | 0.600535 | 3.4:-8834647 | MS.gene046276:CDS |
| ACGAGTATGTGGGAAAACAT+TGG | 0.606382 | 3.4:+8833893 | None:intergenic |
| AGACTCAGAAGACGTTTGAG+TGG | 0.616471 | 3.4:-8834004 | MS.gene046276:CDS |
| AATAATAACAGATCAAGACT+TGG | 0.616986 | 3.4:-8833934 | MS.gene046276:CDS |
| TTTCCTGTAGAGGAAGACTC+TGG | 0.627574 | 3.4:+8833087 | None:intergenic |
| ATGAAGAAGGAGTACCTACA+GGG | 0.636491 | 3.4:-8834394 | MS.gene046276:CDS |
| CTTCATCAAACTTCTCAACG+AGG | 0.637235 | 3.4:+8834411 | None:intergenic |
| CTAAGAGTGTGGCCTATCTG+CGG | 0.648365 | 3.4:-8834466 | MS.gene046276:CDS |
| GTACATGGATAATGAGTGTG+TGG | 0.666454 | 3.4:-8834891 | None:intergenic |
| TGGACAAAGGATGCTAACAA+AGG | 0.673725 | 3.4:-8833132 | MS.gene046276:CDS |
| AATGGTTAGTCCTAAGAGTG+TGG | 0.677899 | 3.4:-8834477 | MS.gene046276:CDS |
| GTCTTCTAAACACTCAACAG+TGG | 0.678815 | 3.4:+8834862 | None:intergenic |
| TATGGAATTTGACTCCATTG+CGG | 0.693460 | 3.4:-8834750 | MS.gene046276:CDS |
| ACATGGCTAGACGCAATGGG+TGG | 0.716265 | 3.4:-8833972 | MS.gene046276:CDS |
| ATAACCGGTGACGAGTATGT+GGG | 0.725095 | 3.4:+8833883 | None:intergenic |
| CCGGCATGAACACTACACAA+AGG | 0.746468 | 3.4:-8833623 | MS.gene046276:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAAGATTATTCAATTT+AGG | - | chr3.4:8834391-8834410 | MS.gene046276:CDS | 10.0% |
| !! | ATTAACAAAAGAATCAAAAA+AGG | + | chr3.4:8834184-8834203 | None:intergenic | 15.0% |
| !! | TGTATTGCATAAAAAAAATT+GGG | + | chr3.4:8833544-8833563 | None:intergenic | 15.0% |
| !! | TTGTATTGCATAAAAAAAAT+TGG | + | chr3.4:8833545-8833564 | None:intergenic | 15.0% |
| !!! | ATATATACAAGAAACATTTT+TGG | - | chr3.4:8834351-8834370 | MS.gene046276:CDS | 15.0% |
| !!! | TATATACAAGAAACATTTTT+GGG | - | chr3.4:8834352-8834371 | MS.gene046276:CDS | 15.0% |
| !! | AAAGATTATTCAATTTAGGA+GGG | - | chr3.4:8834395-8834414 | MS.gene046276:CDS | 20.0% |
| !! | AATGGTTGATTATAATTTGA+AGG | - | chr3.4:8834044-8834063 | MS.gene046276:CDS | 20.0% |
| !! | ATAAACAATGTTCATAATCA+TGG | - | chr3.4:8833265-8833284 | MS.gene046276:CDS | 20.0% |
| !! | ATAGAATGATAAATTTCTTC+TGG | - | chr3.4:8833578-8833597 | MS.gene046276:CDS | 20.0% |
| !! | TAAAGATTATTCAATTTAGG+AGG | - | chr3.4:8834394-8834413 | MS.gene046276:CDS | 20.0% |
| !! | TAGAATGATAAATTTCTTCT+GGG | - | chr3.4:8833579-8833598 | MS.gene046276:CDS | 20.0% |
| !!! | ATACAAAAAAGCTTTGATAA+TGG | - | chr3.4:8834812-8834831 | MS.gene046276:CDS | 20.0% |
| !!! | CATTTTCAATCTATCTTATT+TGG | - | chr3.4:8833718-8833737 | MS.gene046276:CDS | 20.0% |
| !!! | TTTTGTAGTTAAATTTGACA+AGG | - | chr3.4:8834224-8834243 | MS.gene046276:CDS | 20.0% |
| ! | AATAATAACAGATCAAGACT+TGG | - | chr3.4:8833831-8833850 | MS.gene046276:CDS | 25.0% |
| ! | AGTTGTTAAGTCAAAAATTG+AGG | - | chr3.4:8834314-8834333 | MS.gene046276:CDS | 25.0% |
| ! | ATAATTTGAAGGAGAATGAA+TGG | - | chr3.4:8834055-8834074 | MS.gene046276:CDS | 25.0% |
| ! | TATTTGTCCTATTGTTGATA+TGG | - | chr3.4:8832997-8833016 | MS.gene046276:CDS | 25.0% |
| ! | TCAAATTCCATATCAACAAT+AGG | + | chr3.4:8833007-8833026 | None:intergenic | 25.0% |
| ! | TGTACGATATAAGAAAATCA+TGG | - | chr3.4:8834088-8834107 | MS.gene046276:CDS | 25.0% |
| ! | TTGAAAATGATGATTCAATC+CGG | + | chr3.4:8833707-8833726 | None:intergenic | 25.0% |
| !! | AGAATACTTTGAAGAAGAAT+GGG | - | chr3.4:8834014-8834033 | MS.gene046276:CDS | 25.0% |
| !! | TAGAATACTTTGAAGAAGAA+TGG | - | chr3.4:8834013-8834032 | MS.gene046276:CDS | 25.0% |
| !! | TCTTCAAAGTATTCTATAGA+TGG | + | chr3.4:8834009-8834028 | None:intergenic | 25.0% |
| !! | TGAATTTTTGTGGTATATGT+GGG | + | chr3.4:8833947-8833966 | None:intergenic | 25.0% |
| !! | TTGAATTTTTGTGGTATATG+TGG | + | chr3.4:8833948-8833967 | None:intergenic | 25.0% |
| !!! | AAGATAATGCTTTTTCTAGT+CGG | - | chr3.4:8833419-8833438 | MS.gene046276:CDS | 25.0% |
| !!! | ACATGTATATTTTAAAGCGT+TGG | - | chr3.4:8834613-8834632 | MS.gene046276:CDS | 25.0% |
| !!! | AGATTGGCATATCAATTTTT+TGG | - | chr3.4:8833616-8833635 | MS.gene046276:CDS | 25.0% |
| !!! | TTCTTCTGAAATTTCTAAGA+TGG | + | chr3.4:8833456-8833475 | None:intergenic | 25.0% |
| AAAGTTGCTACAATGTTTCA+GGG | - | chr3.4:8833394-8833413 | MS.gene046276:CDS | 30.0% | |
| AAATGGCATATCATACTTGT+TGG | + | chr3.4:8833674-8833693 | None:intergenic | 30.0% | |
| AATGATAAATTTCTTCTGGG+TGG | - | chr3.4:8833582-8833601 | MS.gene046276:CDS | 30.0% | |
| AATTTATGGGATTGCTATGT+AGG | - | chr3.4:8834544-8834563 | MS.gene046276:CDS | 30.0% | |
| AGAAGAAGAAATCTTGATGT+GGG | - | chr3.4:8833469-8833488 | MS.gene046276:CDS | 30.0% | |
| AGGGAAAAACTTGAAAAAGA+AGG | - | chr3.4:8834414-8834433 | MS.gene046276:CDS | 30.0% | |
| AGTTAAATTTGACAAGGCAA+TGG | - | chr3.4:8834230-8834249 | MS.gene046276:CDS | 30.0% | |
| ATCCCATAAATTGAAAAAGC+TGG | + | chr3.4:8834536-8834555 | None:intergenic | 30.0% | |
| CAATGTTCATAATCATGGAA+TGG | - | chr3.4:8833270-8833289 | MS.gene046276:CDS | 30.0% | |
| CTATGAAAAACAAAGAAGAG+AGG | - | chr3.4:8834260-8834279 | MS.gene046276:CDS | 30.0% | |
| GGGAAAAACTTGAAAAAGAA+GGG | - | chr3.4:8834415-8834434 | MS.gene046276:CDS | 30.0% | |
| TAAAGGGTGAAAATGAAGAA+GGG | - | chr3.4:8833152-8833171 | MS.gene046276:CDS | 30.0% | |
| TAAGCTGATATCATCTGATT+TGG | - | chr3.4:8834782-8834801 | MS.gene046276:CDS | 30.0% | |
| TGATCTGTTATTATTGACAC+TGG | + | chr3.4:8833826-8833845 | None:intergenic | 30.0% | |
| TTATGTGCCATAAACATAAC+CGG | + | chr3.4:8833900-8833919 | None:intergenic | 30.0% | |
| ! | ATAGGACAAATATTAGCTTC+AGG | + | chr3.4:8832989-8833008 | None:intergenic | 30.0% |
| ! | ATTCATAGTTTGTGTGGATT+TGG | - | chr3.4:8834485-8834504 | MS.gene046276:CDS | 30.0% |
| ! | GAAGGTACACATATAGTAAA+GGG | - | chr3.4:8833136-8833155 | MS.gene046276:CDS | 30.0% |
| ! | GACTTGGAATTTTCTGAATT+TGG | + | chr3.4:8832965-8832984 | None:intergenic | 30.0% |
| ! | GCTTGAAAGTTGAATTTTTG+TGG | + | chr3.4:8833957-8833976 | None:intergenic | 30.0% |
| ! | TGAAGGTACACATATAGTAA+AGG | - | chr3.4:8833135-8833154 | MS.gene046276:CDS | 30.0% |
| !! | CGCTTTAAAATATACATGTC+TGG | + | chr3.4:8834612-8834631 | None:intergenic | 30.0% |
| !!! | AGATAATGCTTTTTCTAGTC+GGG | - | chr3.4:8833420-8833439 | MS.gene046276:CDS | 30.0% |
| !!! | TATTTTAAAGCGTTGGACAA+AGG | - | chr3.4:8834620-8834639 | MS.gene046276:CDS | 30.0% |
| !!! | TGCCAGCTTTTTCAATTTAT+GGG | - | chr3.4:8834531-8834550 | MS.gene046276:CDS | 30.0% |
| AACTCCAAATCCTTCTTTCT+TGG | + | chr3.4:8833068-8833087 | None:intergenic | 35.0% | |
| AAGGAGAATGAATGGCTTAA+AGG | - | chr3.4:8834063-8834082 | MS.gene046276:CDS | 35.0% | |
| ACACATCATCAACTATCTCA+TGG | + | chr3.4:8832928-8832947 | None:intergenic | 35.0% | |
| AGAAGAATGGGAACGTATAA+TGG | - | chr3.4:8834026-8834045 | MS.gene046276:CDS | 35.0% | |
| AGGAGAATGAATGGCTTAAA+GGG | - | chr3.4:8834064-8834083 | MS.gene046276:CDS | 35.0% | |
| AGGGTCTCAGTATAGATATA+AGG | - | chr3.4:8834434-8834453 | MS.gene046276:CDS | 35.0% | |
| ATAAATTTCTTCTGGGTGGA+TGG | - | chr3.4:8833586-8833605 | MS.gene046276:CDS | 35.0% | |
| ATGTTTGTATGCGCTAATGA+AGG | - | chr3.4:8833118-8833137 | MS.gene046276:CDS | 35.0% | |
| ATTGCTATGTAGGCATATCT+TGG | - | chr3.4:8834554-8834573 | MS.gene046276:CDS | 35.0% | |
| CACTTACTGTTTCAAAAGCA+GGG | - | chr3.4:8833215-8833234 | MS.gene046276:CDS | 35.0% | |
| CAGAAGAAGAAATCTTGATG+TGG | - | chr3.4:8833468-8833487 | MS.gene046276:CDS | 35.0% | |
| GAAAGTTGCTACAATGTTTC+AGG | - | chr3.4:8833393-8833412 | MS.gene046276:CDS | 35.0% | |
| GTAAAGGGTGAAAATGAAGA+AGG | - | chr3.4:8833151-8833170 | MS.gene046276:CDS | 35.0% | |
| GTTGAGAAGTTTGATGAAGA+AGG | - | chr3.4:8833358-8833377 | MS.gene046276:CDS | 35.0% | |
| TATGGAATTTGACTCCATTG+CGG | - | chr3.4:8833015-8833034 | MS.gene046276:CDS | 35.0% | |
| TCACTTACTGTTTCAAAAGC+AGG | - | chr3.4:8833214-8833233 | MS.gene046276:CDS | 35.0% | |
| TGCTTTACAATCAGTTCTTG+AGG | + | chr3.4:8833194-8833213 | None:intergenic | 35.0% | |
| TTTGCCAAGAAAGAAGGATT+TGG | - | chr3.4:8833061-8833080 | MS.gene046276:CDS | 35.0% | |
| ! | AAAGTGCTTCTGTTGAAAAC+AGG | + | chr3.4:8834117-8834136 | None:intergenic | 35.0% |
| ! | AGACACATTCATAGTTTGTG+TGG | - | chr3.4:8834479-8834498 | MS.gene046276:CDS | 35.0% |
| ! | GAAGAGAGGATTTTGAATCA+AGG | - | chr3.4:8834274-8834293 | MS.gene046276:CDS | 35.0% |
| ! | GAATTCTTTTACATCCGCAA+TGG | + | chr3.4:8833032-8833051 | None:intergenic | 35.0% |
| ! | TTGAGTGGTTGTTTAGAACA+TGG | - | chr3.4:8833776-8833795 | MS.gene046276:CDS | 35.0% |
| !! | AGATTTTGACAACTTGCATC+TGG | + | chr3.4:8834873-8834892 | None:intergenic | 35.0% |
| !!! | AACAGAAGCACTTTTTTTGC+CGG | - | chr3.4:8834123-8834142 | MS.gene046276:CDS | 35.0% |
| !!! | CTGCCAGCTTTTTCAATTTA+TGG | - | chr3.4:8834530-8834549 | MS.gene046276:CDS | 35.0% |
| AAGCAGGGAAGAGAAGTAAA+TGG | - | chr3.4:8833230-8833249 | MS.gene046276:CDS | 40.0% | |
| ACATCGTTTGCCAAGAAAGA+AGG | - | chr3.4:8833055-8833074 | MS.gene046276:CDS | 40.0% | |
| ACGAGTATGTGGGAAAACAT+TGG | + | chr3.4:8833875-8833894 | None:intergenic | 40.0% | |
| AGCAGGGAAGAGAAGTAAAT+GGG | - | chr3.4:8833231-8833250 | MS.gene046276:CDS | 40.0% | |
| ATGATGATTCAATCCGGTGA+AGG | + | chr3.4:8833701-8833720 | None:intergenic | 40.0% | |
| ATTGTAGCAACTTTCCCTGT+AGG | + | chr3.4:8833388-8833407 | None:intergenic | 40.0% | |
| CTTCATCAAACTTCTCAACG+AGG | + | chr3.4:8833357-8833376 | None:intergenic | 40.0% | |
| GTCTTCTAAACACTCAACAG+TGG | + | chr3.4:8832906-8832925 | None:intergenic | 40.0% | |
| TGATGATTCAATCCGGTGAA+GGG | + | chr3.4:8833700-8833719 | None:intergenic | 40.0% | |
| TGGACAAAGGATGCTAACAA+AGG | - | chr3.4:8834633-8834652 | MS.gene046276:CDS | 40.0% | |
| TTGTGTGGATTTGGAGACAA+AGG | - | chr3.4:8834494-8834513 | MS.gene046276:CDS | 40.0% | |
| TTTCTTATGAGACCGCAGAT+AGG | + | chr3.4:8833314-8833333 | None:intergenic | 40.0% | |
| ! | AAGGATTTGGAGTTCGTGTT+CGG | - | chr3.4:8833074-8833093 | MS.gene046276:CDS | 40.0% |
| ! | AATATTAGCTTCAGGCGACT+TGG | + | chr3.4:8832981-8833000 | None:intergenic | 40.0% |
| ! | AATGGTTAGTCCTAAGAGTG+TGG | - | chr3.4:8833288-8833307 | MS.gene046276:CDS | 40.0% |
| ! | ATGTGGGAAAACATTGGCTA+TGG | + | chr3.4:8833869-8833888 | None:intergenic | 40.0% |
| ! | GGTGCTGTGTTTATTGAAGA+TGG | - | chr3.4:8834654-8834673 | MS.gene046276:CDS | 40.0% |
| ! | TCAGATTTTGCTGCAAGATC+AGG | + | chr3.4:8834753-8834772 | None:intergenic | 40.0% |
| ! | TTTTGACAACTTGCATCTGG+AGG | + | chr3.4:8834870-8834889 | None:intergenic | 40.0% |
| !! | AGTTGATGAGTTTCCTGTAG+AGG | + | chr3.4:8834691-8834710 | None:intergenic | 40.0% |
| !! | ATGAAGAAGGAGTACCTACA+GGG | - | chr3.4:8833371-8833390 | MS.gene046276:CDS | 40.0% |
| !! | GCTGATATCATCTGATTTGG+AGG | - | chr3.4:8834785-8834804 | MS.gene046276:CDS | 40.0% |
| AGAACATGGCTAGACGCAAT+GGG | - | chr3.4:8833790-8833809 | MS.gene046276:CDS | 45.0% | |
| AGACTCAGAAGACGTTTGAG+TGG | - | chr3.4:8833761-8833780 | MS.gene046276:CDS | 45.0% | |
| ATAACCGGTGACGAGTATGT+GGG | + | chr3.4:8833885-8833904 | None:intergenic | 45.0% | |
| CCTTTGTGTAGTGTTCATGC+CGG | + | chr3.4:8834145-8834164 | None:intergenic | 45.0% | |
| TAGAACATGGCTAGACGCAA+TGG | - | chr3.4:8833789-8833808 | MS.gene046276:CDS | 45.0% | |
| TTTCCTGTAGAGGAAGACTC+TGG | + | chr3.4:8834681-8834700 | None:intergenic | 45.0% | |
| ! | CTCGTCACCGGTTATGTTTA+TGG | - | chr3.4:8833890-8833909 | MS.gene046276:CDS | 45.0% |
| ! | TTTTCCCACATACTCGTCAC+CGG | - | chr3.4:8833878-8833897 | MS.gene046276:CDS | 45.0% |
| !! | CTTTTTCTAGTCGGGATTGC+TGG | - | chr3.4:8833428-8833447 | MS.gene046276:CDS | 45.0% |
| !! | GATGAAGAAGGAGTACCTAC+AGG | - | chr3.4:8833370-8833389 | MS.gene046276:CDS | 45.0% |
| CATAACCGGTGACGAGTATG+TGG | + | chr3.4:8833886-8833905 | None:intergenic | 50.0% | |
| CCGGCATGAACACTACACAA+AGG | - | chr3.4:8834142-8834161 | MS.gene046276:CDS | 50.0% | |
| GGTGGATGGAAGAGCAAGAT+TGG | - | chr3.4:8833600-8833619 | MS.gene046276:CDS | 50.0% | |
| ! | AATCCGGTGAAGGGTGCAAA+TGG | + | chr3.4:8833691-8833710 | None:intergenic | 50.0% |
| ! | ATGCCATTTGCACCCTTCAC+CGG | - | chr3.4:8833685-8833704 | MS.gene046276:CDS | 50.0% |
| ! | CTAAGAGTGTGGCCTATCTG+CGG | - | chr3.4:8833299-8833318 | MS.gene046276:CDS | 50.0% |
| ACATGGCTAGACGCAATGGG+TGG | - | chr3.4:8833793-8833812 | MS.gene046276:CDS | 55.0% | |
| CGCAGATAGGCCACACTCTT+AGG | + | chr3.4:8833301-8833320 | None:intergenic | 55.0% | |
| GGACCAGAGTCTTCCTCTAC+AGG | - | chr3.4:8834675-8834694 | MS.gene046276:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 8832878 | 8834909 | 8832878 | ID=MS.gene046276 |
| chr3.4 | mRNA | 8832878 | 8834909 | 8832878 | ID=MS.gene046276.t1;Parent=MS.gene046276 |
| chr3.4 | exon | 8832878 | 8834909 | 8832878 | ID=MS.gene046276.t1.exon1;Parent=MS.gene046276.t1 |
| chr3.4 | CDS | 8832878 | 8834909 | 8832878 | ID=cds.MS.gene046276.t1;Parent=MS.gene046276.t1 |
| Gene Sequence |
| Protein sequence |
