AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene046314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046314.t1 MTR_3g011990 87.814 279 25 1 1 279 27 296 0.0 500
MS.gene046314.t1 MTR_8g466760 84.229 279 36 1 1 279 27 297 1.53e-172 478
MS.gene046314.t1 MTR_3g011970 72.727 286 62 3 1 279 27 303 1.63e-129 370
MS.gene046314.t1 MTR_3g012010 69.072 291 66 6 1 279 27 305 2.88e-128 367
MS.gene046314.t1 MTR_3g009410 65.744 289 79 8 2 279 28 307 1.06e-111 325
MS.gene046314.t1 MTR_5g008310 52.802 339 96 7 1 279 1 335 1.93e-100 297
MS.gene046314.t1 MTR_5g008320 53.116 337 97 6 1 279 1 334 5.30e-93 278
MS.gene046314.t1 MTR_7g026977 56.296 270 84 7 8 269 93 336 8.35e-85 258
MS.gene046314.t1 MTR_7g022960 56.489 262 91 7 27 279 41 288 9.29e-80 243
MS.gene046314.t1 MTR_7g022980 50.178 281 90 9 8 279 33 272 1.27e-65 206
MS.gene046314.t1 MTR_7g023010 46.619 281 92 9 8 279 33 264 1.97e-63 200
MS.gene046314.t1 MTR_5g008280 50.193 259 74 10 33 241 8 261 1.23e-61 199
MS.gene046314.t1 MTR_5g008280 49.630 135 52 5 151 279 263 387 4.12e-22 95.1
MS.gene046314.t1 MTR_5g008390 62.626 99 25 1 61 159 3 89 6.40e-35 121
MS.gene046314.t1 MTR_7g026710 49.682 157 65 6 127 279 14 160 8.56e-30 110
MS.gene046314.t1 MTR_7g026700 48.101 158 67 7 127 279 14 161 1.61e-26 102
MS.gene046314.t1 MTR_8g466770 70.370 54 16 0 74 127 77 130 4.17e-21 87.0
MS.gene046314.t1 MTR_1g013130 39.823 113 53 4 64 162 86 197 5.52e-15 73.2
MS.gene046314.t1 MTR_1g107055 39.583 96 51 2 71 166 63 151 1.06e-12 65.9
MS.gene046314.t1 MTR_4g006575 45.882 85 32 4 69 151 64 136 3.23e-12 65.1
MS.gene046314.t1 MTR_4g006600 45.882 85 32 4 69 151 64 136 3.23e-12 65.1
MS.gene046314.t1 MTR_2g104900 41.935 93 38 3 69 151 78 164 1.63e-11 63.2
MS.gene046314.t1 MTR_3g106230 37.374 99 39 2 66 146 66 159 3.13e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene046314.t1 AT1G80730 37.168 113 55 2 66 163 60 171 1.12e-12 66.2
MS.gene046314.t1 AT5G01860 68.421 38 12 0 71 108 64 101 1.70e-12 65.5
MS.gene046314.t1 AT5G10970 35.780 109 66 3 70 177 100 205 4.60e-12 65.1
MS.gene046314.t1 AT5G25160 43.678 87 44 4 63 145 49 134 7.48e-12 64.3
MS.gene046314.t1 AT5G27880 54.386 57 23 1 74 130 80 133 1.31e-11 63.9
MS.gene046314.t1 AT1G24625 38.614 101 41 4 52 141 28 118 3.26e-11 62.0

Find 64 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCAATGAAGAGAAGAATT+TGG 0.201357 3.4:-8260249 MS.gene046314:CDS
GATGATGGTGCTTCAGGTTT+AGG 0.213401 3.4:-8260104 MS.gene046314:CDS
AAACTGTGTGGGGTAATCTT+AGG 0.228026 3.4:+8259984 None:intergenic
AAGATTACCCCACACAGTTT+TGG 0.280652 3.4:-8259981 MS.gene046314:CDS
ATGATGGTGCTTCAGGTTTA+GGG 0.292878 3.4:-8260103 MS.gene046314:CDS
GTTCCGCCTTGTGAGCATTC+TGG 0.293208 3.4:+8260159 None:intergenic
ATTGGTGCAATGCTAAAATT+AGG 0.304816 3.4:-8259786 MS.gene046314:CDS
AGGTATGATGATGGTGCTTC+AGG 0.309881 3.4:-8260110 MS.gene046314:CDS
GGTGGTACAAATAATAAAAT+TGG 0.315699 3.4:-8259804 MS.gene046314:CDS
TTTCCTTGATTGTTGTCATT+AGG 0.336005 3.4:+8260281 None:intergenic
AGGGGTGGTAACAGCGGGTT+TGG 0.340009 3.4:-8259894 MS.gene046314:CDS
TAGATCATGAATACCATCTT+TGG 0.348347 3.4:+8259689 None:intergenic
TGACAACTTCATGAAATCAA+TGG 0.381820 3.4:+8260385 None:intergenic
GTAATCTTAGGGCTAAAATA+AGG 0.401113 3.4:+8259996 None:intergenic
CATGAAATCAATGGGTTTGT+CGG 0.413662 3.4:+8260394 None:intergenic
TTGCTTCATTGAGAAATATG+AGG 0.417907 3.4:-8259914 MS.gene046314:CDS
GACAACTTCATGAAATCAAT+GGG 0.421311 3.4:+8260386 None:intergenic
GACGATGAACCAATTTGAAT+GGG 0.422269 3.4:+8260311 None:intergenic
ATGAAATCAATGGGTTTGTC+GGG 0.431271 3.4:+8260395 None:intergenic
TTTCCTAATGACAACAATCA+AGG 0.441970 3.4:-8260284 MS.gene046314:CDS
ATTCTCTACTTCACAAGCAT+TGG 0.443427 3.4:-8260189 MS.gene046314:CDS
TGCTCTTGCAACTTCGACAC+GGG 0.458755 3.4:+8260347 None:intergenic
TTCTCTACTTCACAAGCATT+GGG 0.462768 3.4:-8260188 MS.gene046314:CDS
AACTGTGTGGGGTAATCTTA+GGG 0.464573 3.4:+8259985 None:intergenic
AGAGAATTGTCTCTTACAAA+AGG 0.466075 3.4:+8260205 None:intergenic
AGACGATGAACCAATTTGAA+TGG 0.482754 3.4:+8260310 None:intergenic
CCTTATGATTATAGTTCACT+CGG 0.486258 3.4:-8260050 MS.gene046314:CDS
AAGGTGGTGCAAGTACTTCA+AGG 0.489306 3.4:-8259833 MS.gene046314:CDS
CCGAGTGAACTATAATCATA+AGG 0.501901 3.4:+8260050 None:intergenic
GCTCCTACTTCTACCAAAGA+TGG 0.531766 3.4:-8259702 MS.gene046314:CDS
TCGGTGGTGCAAGTACTTCA+AGG 0.532445 3.4:-8259860 MS.gene046314:CDS
TGCTTCATTGAGAAATATGA+GGG 0.532522 3.4:-8259913 MS.gene046314:CDS
CTTATGATTATAGTTCACTC+GGG 0.536404 3.4:-8260049 MS.gene046314:CDS
TTTCTCAATGAAGCAAGAGA+AGG 0.547522 3.4:+8259921 None:intergenic
TTCTCAATGAAGCAAGAGAA+GGG 0.558954 3.4:+8259922 None:intergenic
GACGGTACCCAAAACTGTGT+GGG 0.561677 3.4:+8259973 None:intergenic
GCAAGTACTTCAAGGATTGA+AGG 0.568493 3.4:-8259825 MS.gene046314:CDS
GCAAGTACTTCAAGGATTGA+AGG 0.568493 3.4:-8259852 MS.gene046314:CDS
ATATGAGGGGTGGTAACAGC+GGG 0.569644 3.4:-8259899 MS.gene046314:CDS
GTGCTCTTGCAACTTCGACA+CGG 0.570780 3.4:+8260346 None:intergenic
CGGAAACAAAGGTATGATGA+TGG 0.586182 3.4:-8260119 MS.gene046314:CDS
GGGTTTGGAAACCTAGGTAT+CGG 0.586346 3.4:-8259879 MS.gene046314:CDS
TAGAAAAGCAACGGAAACAA+AGG 0.606956 3.4:-8260130 MS.gene046314:CDS
TACTTGCACCACCGATACCT+AGG 0.614177 3.4:+8259868 None:intergenic
ATACCATCTTTGGTAGAAGT+AGG 0.614243 3.4:+8259699 None:intergenic
TGACGGTACCCAAAACTGTG+TGG 0.621693 3.4:+8259972 None:intergenic
TGGTATTCATGATCTATCAA+AGG 0.623851 3.4:-8259682 MS.gene046314:CDS
ATCTTAGGGCTAAAATAAGG+TGG 0.624011 3.4:+8259999 None:intergenic
TCAGATGATTCAGAATCAGA+AGG 0.636973 3.4:+8259642 None:intergenic
CTTGCAACAAAGCACTATGA+CGG 0.651264 3.4:+8259955 None:intergenic
TCATTGAGAAATATGAGGGG+TGG 0.653640 3.4:-8259909 MS.gene046314:CDS
TTTGGAAACCTAGGTATCGG+TGG 0.657683 3.4:-8259876 MS.gene046314:CDS
AACGTGCTATAGAAAAGCAA+CGG 0.662928 3.4:-8260139 MS.gene046314:CDS
TCTACTTCACAAGCATTGGG+AGG 0.664622 3.4:-8260185 MS.gene046314:CDS
ACGGTACCCAAAACTGTGTG+GGG 0.666056 3.4:+8259974 None:intergenic
AATATGAGGGGTGGTAACAG+CGG 0.669747 3.4:-8259900 MS.gene046314:CDS
AACAGCGGGTTTGGAAACCT+AGG 0.670930 3.4:-8259885 MS.gene046314:CDS
AGGACACCAGAATGCTCACA+AGG 0.673314 3.4:-8260165 MS.gene046314:CDS
TTATGATTATAGTTCACTCG+GGG 0.683240 3.4:-8260048 MS.gene046314:CDS
ACGATGAACCAATTTGAATG+GGG 0.690761 3.4:+8260312 None:intergenic
AGTACTTCAAGGATTGAAGG+TGG 0.697813 3.4:-8259849 MS.gene046314:CDS
AGTACTTCAAGGATTGAAGG+TGG 0.714441 3.4:-8259822 MS.gene046314:CDS
ACACCAGAATGCTCACAAGG+CGG 0.738475 3.4:-8260162 MS.gene046314:CDS
GCTTCATTGAGAAATATGAG+GGG 0.751842 3.4:-8259912 MS.gene046314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAGAAAAACAAATTATTG+TGG - chr3.4:8260327-8260346 MS.gene046314:CDS 20.0%
! GGTGGTACAAATAATAAAAT+TGG - chr3.4:8260247-8260266 MS.gene046314:CDS 25.0%
AGAGAATTGTCTCTTACAAA+AGG + chr3.4:8259849-8259868 None:intergenic 30.0%
ATTGGTGCAATGCTAAAATT+AGG - chr3.4:8260265-8260284 MS.gene046314:CDS 30.0%
CCTTATGATTATAGTTCACT+CGG - chr3.4:8260001-8260020 MS.gene046314:CDS 30.0%
CTTATGATTATAGTTCACTC+GGG - chr3.4:8260002-8260021 MS.gene046314:CDS 30.0%
GACAACTTCATGAAATCAAT+GGG + chr3.4:8259668-8259687 None:intergenic 30.0%
GTAATCTTAGGGCTAAAATA+AGG + chr3.4:8260058-8260077 None:intergenic 30.0%
TAGATCATGAATACCATCTT+TGG + chr3.4:8260365-8260384 None:intergenic 30.0%
TGACAACTTCATGAAATCAA+TGG + chr3.4:8259669-8259688 None:intergenic 30.0%
TGCTTCATTGAGAAATATGA+GGG - chr3.4:8260138-8260157 MS.gene046314:CDS 30.0%
TGGTATTCATGATCTATCAA+AGG - chr3.4:8260369-8260388 MS.gene046314:CDS 30.0%
TTATGATTATAGTTCACTCG+GGG - chr3.4:8260003-8260022 MS.gene046314:CDS 30.0%
TTGCTTCATTGAGAAATATG+AGG - chr3.4:8260137-8260156 MS.gene046314:CDS 30.0%
TTTCCTAATGACAACAATCA+AGG - chr3.4:8259767-8259786 MS.gene046314:CDS 30.0%
TTTCCTTGATTGTTGTCATT+AGG + chr3.4:8259773-8259792 None:intergenic 30.0%
!! TGGTTTTTGAATCATTGACT+CGG + chr3.4:8260035-8260054 None:intergenic 30.0%
!!! TTGCAAGAGCACAATTTTTT+CGG - chr3.4:8259716-8259735 MS.gene046314:CDS 30.0%
AACGTGCTATAGAAAAGCAA+CGG - chr3.4:8259912-8259931 MS.gene046314:CDS 35.0%
ACGATGAACCAATTTGAATG+GGG + chr3.4:8259742-8259761 None:intergenic 35.0%
AGACGATGAACCAATTTGAA+TGG + chr3.4:8259744-8259763 None:intergenic 35.0%
ATACCATCTTTGGTAGAAGT+AGG + chr3.4:8260355-8260374 None:intergenic 35.0%
ATCTTAGGGCTAAAATAAGG+TGG + chr3.4:8260055-8260074 None:intergenic 35.0%
ATTCTCTACTTCACAAGCAT+TGG - chr3.4:8259862-8259881 MS.gene046314:CDS 35.0%
CCGAGTGAACTATAATCATA+AGG + chr3.4:8260004-8260023 None:intergenic 35.0%
CGTCAATGAAGAGAAGAATT+TGG - chr3.4:8259802-8259821 MS.gene046314:CDS 35.0%
GACGATGAACCAATTTGAAT+GGG + chr3.4:8259743-8259762 None:intergenic 35.0%
GCTTCATTGAGAAATATGAG+GGG - chr3.4:8260139-8260158 MS.gene046314:CDS 35.0%
TAGAAAAGCAACGGAAACAA+AGG - chr3.4:8259921-8259940 MS.gene046314:CDS 35.0%
TCAGATGATTCAGAATCAGA+AGG + chr3.4:8260412-8260431 None:intergenic 35.0%
TCATAAGGTCGAAAAAGAGT+AGG + chr3.4:8259989-8260008 None:intergenic 35.0%
TTCTCAATGAAGCAAGAGAA+GGG + chr3.4:8260132-8260151 None:intergenic 35.0%
TTCTCTACTTCACAAGCATT+GGG - chr3.4:8259863-8259882 MS.gene046314:CDS 35.0%
TTTCTCAATGAAGCAAGAGA+AGG + chr3.4:8260133-8260152 None:intergenic 35.0%
! ATGAAATCAATGGGTTTGTC+GGG + chr3.4:8259659-8259678 None:intergenic 35.0%
! CATGAAATCAATGGGTTTGT+CGG + chr3.4:8259660-8259679 None:intergenic 35.0%
AAACTGTGTGGGGTAATCTT+AGG + chr3.4:8260070-8260089 None:intergenic 40.0%
AACTGTGTGGGGTAATCTTA+GGG + chr3.4:8260069-8260088 None:intergenic 40.0%
AGTACTTCAAGGATTGAAGG+TGG - chr3.4:8260202-8260221 MS.gene046314:CDS 40.0%
GCAAGTACTTCAAGGATTGA+AGG - chr3.4:8260199-8260218 MS.gene046314:CDS 40.0%
TCATTGAGAAATATGAGGGG+TGG - chr3.4:8260142-8260161 MS.gene046314:CDS 40.0%
! AAGATTACCCCACACAGTTT+TGG - chr3.4:8260070-8260089 MS.gene046314:CDS 40.0%
! AGATTACCCCACACAGTTTT+GGG - chr3.4:8260071-8260090 MS.gene046314:CDS 40.0%
! ATGATGGTGCTTCAGGTTTA+GGG - chr3.4:8259948-8259967 MS.gene046314:CDS 40.0%
! CGGAAACAAAGGTATGATGA+TGG - chr3.4:8259932-8259951 MS.gene046314:CDS 40.0%
! CTTGCAACAAAGCACTATGA+CGG + chr3.4:8260099-8260118 None:intergenic 40.0%
!! TTTTTCGGCCCCATTCAAAT+TGG - chr3.4:8259731-8259750 MS.gene046314:CDS 40.0%
!!! TTTGTCGGGTTTTGAGTTTG+AGG + chr3.4:8259645-8259664 None:intergenic 40.0%
AAGGTGGTGCAAGTACTTCA+AGG - chr3.4:8260218-8260237 MS.gene046314:CDS 45.0%
AATATGAGGGGTGGTAACAG+CGG - chr3.4:8260151-8260170 MS.gene046314:CDS 45.0%
GCTCCTACTTCTACCAAAGA+TGG - chr3.4:8260349-8260368 MS.gene046314:CDS 45.0%
GGGTTTGGAAACCTAGGTAT+CGG - chr3.4:8260172-8260191 MS.gene046314:CDS 45.0%
! GATGATGGTGCTTCAGGTTT+AGG - chr3.4:8259947-8259966 MS.gene046314:CDS 45.0%
!! AGGTATGATGATGGTGCTTC+AGG - chr3.4:8259941-8259960 MS.gene046314:CDS 45.0%
!! TCTACTTCACAAGCATTGGG+AGG - chr3.4:8259866-8259885 MS.gene046314:CDS 45.0%
!! TTTGGAAACCTAGGTATCGG+TGG - chr3.4:8260175-8260194 MS.gene046314:CDS 45.0%
ACACCAGAATGCTCACAAGG+CGG - chr3.4:8259889-8259908 MS.gene046314:CDS 50.0%
ACGGTACCCAAAACTGTGTG+GGG + chr3.4:8260080-8260099 None:intergenic 50.0%
AGGACACCAGAATGCTCACA+AGG - chr3.4:8259886-8259905 MS.gene046314:CDS 50.0%
ATATGAGGGGTGGTAACAGC+GGG - chr3.4:8260152-8260171 MS.gene046314:CDS 50.0%
GACGGTACCCAAAACTGTGT+GGG + chr3.4:8260081-8260100 None:intergenic 50.0%
GTGCTCTTGCAACTTCGACA+CGG + chr3.4:8259708-8259727 None:intergenic 50.0%
TACTTGCACCACCGATACCT+AGG + chr3.4:8260186-8260205 None:intergenic 50.0%
TCGGTGGTGCAAGTACTTCA+AGG - chr3.4:8260191-8260210 MS.gene046314:CDS 50.0%
TGACGGTACCCAAAACTGTG+TGG + chr3.4:8260082-8260101 None:intergenic 50.0%
TGCTCTTGCAACTTCGACAC+GGG + chr3.4:8259707-8259726 None:intergenic 50.0%
! AACAGCGGGTTTGGAAACCT+AGG - chr3.4:8260166-8260185 MS.gene046314:CDS 50.0%
GTTCCGCCTTGTGAGCATTC+TGG + chr3.4:8259895-8259914 None:intergenic 55.0%
! AGGGGTGGTAACAGCGGGTT+TGG - chr3.4:8260157-8260176 MS.gene046314:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 8259617 8260456 8259617 ID=MS.gene046314
chr3.4 mRNA 8259617 8260456 8259617 ID=MS.gene046314.t1;Parent=MS.gene046314
chr3.4 exon 8259617 8260456 8259617 ID=MS.gene046314.t1.exon1;Parent=MS.gene046314.t1
chr3.4 CDS 8259617 8260456 8259617 ID=cds.MS.gene046314.t1;Parent=MS.gene046314.t1
Gene Sequence

>MS.gene046314

ATGAAGGAGATTGAACACATTCAATCCTCAAACTCAAAACCCGACAAACCCATTGATTTCATGAAGTTGTCAAAAGACGATTCAATTCCCGTGTCGAAGTTGCAAGAGCACAATTTTTTCGGCCCCATTCAAATTGGTTCATCGTCTTGTTTTCCTAATGACAACAATCAAGGAAAAGATGAGAACGTCAATGAAGAGAAGAATTTGGATTCAAGATCTTTTTCATGCTCCTTTTGTAAGAGACAATTCTCTACTTCACAAGCATTGGGAGGACACCAGAATGCTCACAAGGCGGAACGTGCTATAGAAAAGCAACGGAAACAAAGGTATGATGATGGTGCTTCAGGTTTAGGGCAATCTCACTTTAATCCTACTCTTTTTCGACCTTATGATTATAGTTCACTCGGGGTTAGAACCGAGTCAATGATTCAAAAACCACCTTATTTTAGCCCTAAGATTACCCCACACAGTTTTGGGTACCGTCATAGTGCTTTGTTGCAAGAGATCTTAAACCCTTCTCTTGCTTCATTGAGAAATATGAGGGGTGGTAACAGCGGGTTTGGAAACCTAGGTATCGGTGGTGCAAGTACTTCAAGGATTGAAGGTGGTGCAAGTACTTCAAGGATTGAAGGTGGTACAAATAATAAAATTGGTGCAATGCTAAAATTAGGAGATTCTTCTACAAATATTGCTACAAGTTCAAACTCAAACATAGAAAAACAAATTATTGTGGCTCCTACTTCTACCAAAGATGGTATTCATGATCTATCAAAGGCTAACATTGAAGAAGAACCTTCTGATTCTGAATCATCTGAGCTTGATTTATCACTCAAGCTTTAA

Protein sequence

>MS.gene046314.t1

MKEIEHIQSSNSKPDKPIDFMKLSKDDSIPVSKLQEHNFFGPIQIGSSSCFPNDNNQGKDENVNEEKNLDSRSFSCSFCKRQFSTSQALGGHQNAHKAERAIEKQRKQRYDDGASGLGQSHFNPTLFRPYDYSSLGVRTESMIQKPPYFSPKITPHSFGYRHSALLQEILNPSLASLRNMRGGNSGFGNLGIGGASTSRIEGGASTSRIEGGTNNKIGAMLKLGDSSTNIATSSNSNIEKQIIVAPTSTKDGIHDLSKANIEEEPSDSESSELDLSLKL