Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046452.t1 | PNY07851.1 | 35.2 | 213 | 136 | 2 | 13 | 223 | 12 | 224 | 3.30E-32 | 148.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046452.t1 | A0A2K3NXT8 | 35.2 | 213 | 136 | 2 | 13 | 223 | 12 | 224 | 2.4e-32 | 148.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046452.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046452.t1 | MTR_4g123470 | 28.141 | 199 | 143 | 0 | 10 | 208 | 12 | 210 | 5.30e-28 | 106 |
| MS.gene046452.t1 | MTR_0014s0220 | 26.108 | 203 | 132 | 5 | 12 | 213 | 628 | 813 | 1.56e-15 | 75.5 |
| MS.gene046452.t1 | MTR_1g054495 | 22.798 | 193 | 148 | 1 | 17 | 209 | 16 | 207 | 2.37e-15 | 72.8 |
| MS.gene046452.t1 | MTR_4g085790 | 24.121 | 199 | 150 | 1 | 25 | 223 | 24 | 221 | 1.96e-14 | 70.1 |
| MS.gene046452.t1 | MTR_7g025150 | 24.103 | 195 | 147 | 1 | 29 | 223 | 28 | 221 | 2.52e-14 | 69.7 |
| MS.gene046452.t1 | MTR_1g090753 | 32.632 | 95 | 64 | 0 | 22 | 116 | 38 | 132 | 5.09e-14 | 67.8 |
| MS.gene046452.t1 | MTR_4g053550 | 45.714 | 70 | 38 | 0 | 27 | 96 | 49 | 118 | 6.32e-14 | 66.6 |
| MS.gene046452.t1 | MTR_5g074790 | 31.731 | 104 | 71 | 0 | 14 | 117 | 160 | 263 | 1.57e-12 | 65.5 |
| MS.gene046452.t1 | MTR_1g054485 | 23.353 | 167 | 128 | 0 | 22 | 188 | 21 | 187 | 3.30e-12 | 63.2 |
| MS.gene046452.t1 | MTR_7g039210 | 20.548 | 219 | 143 | 4 | 12 | 199 | 10 | 228 | 7.30e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAATGTGATGTAATAATA+AGG | - | 57139:21693-21712 | MS.gene046452:intron | 20.0% |
| !! | CAAATGTGATGTAATAATAA+GGG | - | 57139:21694-21713 | MS.gene046452:intron | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | - | 57139:21755-21774 | MS.gene046452:intron | 20.0% |
| ! | AAGTACATCAGATATAATGA+AGG | - | 57139:21820-21839 | MS.gene046452:CDS | 25.0% |
| ! | AATAAGGGATAGAAAAAAGA+AGG | - | 57139:21709-21728 | MS.gene046452:intron | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | - | 57139:21361-21380 | MS.gene046452:CDS | 25.0% |
| ! | AGTGCTCAACATAAAATAAT+AGG | + | 57139:21113-21132 | None:intergenic | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | - | 57139:21362-21381 | MS.gene046452:CDS | 25.0% |
| ! | TACTCTGCTATTAATATGTA+TGG | - | 57139:21653-21672 | MS.gene046452:CDS | 25.0% |
| ! | TATTACATCACATTTGTACT+CGG | + | 57139:21690-21709 | None:intergenic | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | - | 57139:21793-21812 | MS.gene046452:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | - | 57139:21722-21741 | MS.gene046452:intron | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | - | 57139:21490-21509 | MS.gene046452:CDS | 30.0% | |
| ATAAAATGCTCAACAAAGGA+AGG | + | 57139:21200-21219 | None:intergenic | 30.0% | |
| ATATCTCATTTACAGACGTA+TGG | - | 57139:21164-21183 | MS.gene046452:CDS | 30.0% | |
| ATCATTTGAGAATGAGTTGT+TGG | + | 57139:21540-21559 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | - | 57139:21662-21681 | MS.gene046452:CDS | 30.0% | |
| CAGATATAATGAAGGTTTCT+AGG | - | 57139:21828-21847 | MS.gene046452:CDS | 30.0% | |
| GAACTTAACTTTGTGTCTAT+TGG | + | 57139:21264-21283 | None:intergenic | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | + | 57139:21484-21503 | None:intergenic | 30.0% | |
| TAAATTCTCTTCCAAGTTTG+TGG | + | 57139:21223-21242 | None:intergenic | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | - | 57139:21077-21096 | MS.gene046452:CDS | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | - | 57139:21363-21382 | MS.gene046452:CDS | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | - | 57139:21323-21342 | MS.gene046452:intron | 30.0% |
| ATCTACTTCAAGGACTCATA+GGG | - | 57139:21038-21057 | MS.gene046452:CDS | 35.0% | |
| ATGTTAACTCATTGCCATCA+TGG | - | 57139:21426-21445 | MS.gene046452:CDS | 35.0% | |
| GTGGATAAAATGCTCAACAA+AGG | + | 57139:21204-21223 | None:intergenic | 35.0% | |
| TTCCAATTCACTGCATTTCA+TGG | - | 57139:21395-21414 | MS.gene046452:CDS | 35.0% | |
| ! | GAGCATTTTATCCACAAACT+TGG | - | 57139:21209-21228 | MS.gene046452:CDS | 35.0% |
| ! | TGTAAAGACAATGGATTCCA+TGG | - | 57139:21764-21783 | MS.gene046452:intron | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | - | 57139:21327-21346 | MS.gene046452:intron | 35.0% |
| CACTTACAAGCAAGGAATTG+AGG | - | 57139:21451-21470 | MS.gene046452:CDS | 40.0% | |
| GATCTACTTCAAGGACTCAT+AGG | - | 57139:21037-21056 | MS.gene046452:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | - | 57139:21629-21648 | MS.gene046452:CDS | 40.0% | |
| GTCCATGAAATGCAGTGAAT+TGG | + | 57139:21400-21419 | None:intergenic | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | - | 57139:21508-21527 | MS.gene046452:CDS | 40.0% | |
| TCAAGGACTCATAGGGATAA+TGG | - | 57139:21045-21064 | MS.gene046452:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | - | 57139:21313-21332 | MS.gene046452:intron | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | + | 57139:21506-21525 | None:intergenic | 40.0% | |
| TGCAGCCATATAAGGAGTTA+AGG | + | 57139:21498-21517 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | - | 57139:21497-21516 | MS.gene046452:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | - | 57139:21767-21786 | MS.gene046452:intron | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | - | 57139:21768-21787 | MS.gene046452:intron | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | - | 57139:21608-21627 | MS.gene046452:CDS | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | - | 57139:21728-21747 | MS.gene046452:intron | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | + | 57139:21784-21803 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | - | 57139:21729-21748 | MS.gene046452:intron | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | - | 57139:21312-21331 | MS.gene046452:intron | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | - | 57139:21296-21315 | MS.gene046452:intron | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | - | 57139:21443-21462 | MS.gene046452:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | - | 57139:21295-21314 | MS.gene046452:intron | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | + | 57139:21443-21462 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | - | 57139:21769-21788 | MS.gene046452:intron | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | - | 57139:21300-21319 | MS.gene046452:intron | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | + | 57139:21305-21324 | None:intergenic | 50.0% | |
| CAACAAAGATTGGCAGTGGG+TGG | - | 57139:21732-21751 | MS.gene046452:intron | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | + | 57139:21680-21699 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCTACTTCA+AGG | - | 57139:21028-21047 | MS.gene046452:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57139 | gene | 21024 | 21856 | 21024 | ID=MS.gene046452 |
| 57139 | mRNA | 21024 | 21856 | 21024 | ID=MS.gene046452.t1;Parent=MS.gene046452 |
| 57139 | exon | 21782 | 21856 | 21782 | ID=MS.gene046452.t1.exon1;Parent=MS.gene046452.t1 |
| 57139 | CDS | 21782 | 21856 | 21782 | ID=cds.MS.gene046452.t1;Parent=MS.gene046452.t1 |
| 57139 | exon | 21351 | 21701 | 21351 | ID=MS.gene046452.t1.exon2;Parent=MS.gene046452.t1 |
| 57139 | CDS | 21351 | 21701 | 21351 | ID=cds.MS.gene046452.t1;Parent=MS.gene046452.t1 |
| 57139 | exon | 21024 | 21269 | 21024 | ID=MS.gene046452.t1.exon3;Parent=MS.gene046452.t1 |
| 57139 | CDS | 21024 | 21269 | 21024 | ID=cds.MS.gene046452.t1;Parent=MS.gene046452.t1 |
| Gene Sequence |
| Protein sequence |
