Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046492.t1 | XP_024639353.1 | 89.4 | 85 | 9 | 0 | 1 | 85 | 47 | 131 | 8.90E-39 | 169.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046492.t1 | G7JX45 | 89.4 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 6.4e-39 | 169.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046492.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046492.t1 | MTR_5g053510 | 89.412 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 3.10e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACTTCAATGATTTCTTA+TGG | 0.226332 | 8.3:-45503025 | MS.gene046492:CDS |
| GATTTGCATTGCACAGAAAA+AGG | 0.262758 | 8.3:+45502985 | None:intergenic |
| AACGAATAGTGTCTCCAATC+TGG | 0.387174 | 8.3:+45502859 | None:intergenic |
| AGACTTCAATGATTTCTTAT+GGG | 0.387413 | 8.3:-45503024 | MS.gene046492:CDS |
| TATTCAGCGATTCATTGCTT+TGG | 0.421950 | 8.3:+45502928 | None:intergenic |
| GCTGTAACAAACTCTTCATT+CGG | 0.423810 | 8.3:+45502963 | None:intergenic |
| CATAAGAAATCATTGAAGTC+TGG | 0.456714 | 8.3:+45503026 | None:intergenic |
| TTCCTATAGACATGAACAAA+AGG | 0.480398 | 8.3:+45502810 | None:intergenic |
| ATTCGTTTCAAGTTCTTCGA+TGG | 0.500169 | 8.3:-45502843 | MS.gene046492:CDS |
| TTCGTTTCAAGTTCTTCGAT+GGG | 0.505866 | 8.3:-45502842 | MS.gene046492:CDS |
| AACAACAATGCATTCCAGAT+TGG | 0.510902 | 8.3:-45502873 | MS.gene046492:CDS |
| AAGAAATCATTGAAGTCTGG+TGG | 0.620565 | 8.3:+45503029 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAGTTAAAAAATGGT+TGG | - | chr8.3:45502927-45502946 | MS.gene046492:CDS | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+TGG | - | chr8.3:45502923-45502942 | MS.gene046492:CDS | 20.0% |
| !! | TACTAAGTTAAAAAATGGTT+GGG | - | chr8.3:45502928-45502947 | MS.gene046492:CDS | 20.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | - | chr8.3:45502811-45502830 | MS.gene046492:CDS | 25.0% |
| CAGACTTCAATGATTTCTTA+TGG | - | chr8.3:45502810-45502829 | MS.gene046492:CDS | 30.0% | |
| CATAAGAAATCATTGAAGTC+TGG | + | chr8.3:45502812-45502831 | None:intergenic | 30.0% | |
| TTCCTATAGACATGAACAAA+AGG | + | chr8.3:45503028-45503047 | None:intergenic | 30.0% | |
| AACAACAATGCATTCCAGAT+TGG | - | chr8.3:45502962-45502981 | MS.gene046492:CDS | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | + | chr8.3:45502809-45502828 | None:intergenic | 35.0% | |
| GATTTGCATTGCACAGAAAA+AGG | + | chr8.3:45502853-45502872 | None:intergenic | 35.0% | |
| GCTGTAACAAACTCTTCATT+CGG | + | chr8.3:45502875-45502894 | None:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | + | chr8.3:45502910-45502929 | None:intergenic | 35.0% | |
| ! | GTCCTTTTGTTCATGTCTAT+AGG | - | chr8.3:45503023-45503042 | MS.gene046492:CDS | 35.0% |
| !! | ATTCGTTTCAAGTTCTTCGA+TGG | - | chr8.3:45502992-45503011 | MS.gene046492:CDS | 35.0% |
| !! | TTCGTTTCAAGTTCTTCGAT+GGG | - | chr8.3:45502993-45503012 | MS.gene046492:CDS | 35.0% |
| AACGAATAGTGTCTCCAATC+TGG | + | chr8.3:45502979-45502998 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 45502800 | 45503057 | 45502800 | ID=MS.gene046492 |
| chr8.3 | mRNA | 45502800 | 45503057 | 45502800 | ID=MS.gene046492.t1;Parent=MS.gene046492 |
| chr8.3 | exon | 45502800 | 45503057 | 45502800 | ID=MS.gene046492.t1.exon1;Parent=MS.gene046492.t1 |
| chr8.3 | CDS | 45502800 | 45503057 | 45502800 | ID=cds.MS.gene046492.t1;Parent=MS.gene046492.t1 |
| Gene Sequence |
| Protein sequence |
