Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046495.t1 | XP_024639353.1 | 89.4 | 85 | 9 | 0 | 1 | 85 | 47 | 131 | 8.90E-39 | 169.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046495.t1 | G7JX45 | 89.4 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 6.4e-39 | 169.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046495.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046495.t1 | MTR_5g053510 | 89.412 | 85 | 9 | 0 | 1 | 85 | 25 | 109 | 3.10e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAGTTAAAAAATGGT+TGG | - | 57245:12056-12075 | MS.gene046495:CDS | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+TGG | - | 57245:12052-12071 | MS.gene046495:CDS | 20.0% |
| !! | TACTAAGTTAAAAAATGGTT+GGG | - | 57245:12057-12076 | MS.gene046495:CDS | 20.0% |
| ! | AGACTTCAATGATTTCTTAT+GGG | - | 57245:11940-11959 | MS.gene046495:CDS | 25.0% |
| CAGACTTCAATGATTTCTTA+TGG | - | 57245:11939-11958 | MS.gene046495:CDS | 30.0% | |
| CATAAGAAATCATTGAAGTC+TGG | + | 57245:11941-11960 | MS.gene046495:intergenic | 30.0% | |
| TTCCTATAGACATGAACAAA+AGG | + | 57245:12157-12176 | MS.gene046495:intergenic | 30.0% | |
| AACAACAATGCATTCCAGAT+TGG | - | 57245:12091-12110 | MS.gene046495:CDS | 35.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | + | 57245:11938-11957 | MS.gene046495:intergenic | 35.0% | |
| GATTTGCATTGCACAGAAAA+AGG | + | 57245:11982-12001 | MS.gene046495:intergenic | 35.0% | |
| GCTGTAACAAACTCTTCATT+CGG | + | 57245:12004-12023 | MS.gene046495:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | + | 57245:12039-12058 | MS.gene046495:intergenic | 35.0% | |
| ! | GTCCTTTTGTTCATGTCTAT+AGG | - | 57245:12152-12171 | MS.gene046495:CDS | 35.0% |
| !! | ATTCGTTTCAAGTTCTTCGA+TGG | - | 57245:12121-12140 | MS.gene046495:CDS | 35.0% |
| !! | TTCGTTTCAAGTTCTTCGAT+GGG | - | 57245:12122-12141 | MS.gene046495:CDS | 35.0% |
| AACGAATAGTGTCTCCAATC+TGG | + | 57245:12108-12127 | MS.gene046495:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57245 | gene | 11929 | 12186 | 11929 | ID=MS.gene046495 |
| 57245 | mRNA | 11929 | 12186 | 11929 | ID=MS.gene046495.t1;Parent=MS.gene046495 |
| 57245 | exon | 11929 | 12186 | 11929 | ID=MS.gene046495.t1.exon1;Parent=MS.gene046495.t1 |
| 57245 | CDS | 11929 | 12186 | 11929 | ID=cds.MS.gene046495.t1;Parent=MS.gene046495.t1 |
| Gene Sequence |
| Protein sequence |
