Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046496.t1 | KEH31552.1 | 99.2 | 128 | 1 | 0 | 1 | 128 | 1 | 128 | 9.10E-19 | 103.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046496.t1 | Q93ZB1 | 100.0 | 39 | 0 | 0 | 5 | 43 | 32 | 70 | 1.4e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046496.t1 | A0A072UPD4 | 99.2 | 128 | 1 | 0 | 1 | 128 | 1 | 128 | 6.5e-19 | 103.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene046496.t1 | TF | C2C2-LSD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046496.t1 | MTR_4g098500 | 99.219 | 128 | 1 | 0 | 1 | 128 | 1 | 128 | 7.85e-89 | 253 |
| MS.gene046496.t1 | MTR_4g098500 | 99.206 | 126 | 1 | 0 | 3 | 128 | 29 | 154 | 3.76e-88 | 253 |
| MS.gene046496.t1 | MTR_8g089310 | 73.451 | 113 | 30 | 0 | 5 | 117 | 2 | 114 | 5.42e-58 | 177 |
| MS.gene046496.t1 | MTR_8g089310 | 73.451 | 113 | 30 | 0 | 5 | 117 | 15 | 127 | 7.06e-58 | 177 |
| MS.gene046496.t1 | MTR_8g089310 | 73.451 | 113 | 30 | 0 | 5 | 117 | 49 | 161 | 1.37e-57 | 178 |
| MS.gene046496.t1 | MTR_2g047838 | 60.526 | 114 | 42 | 2 | 5 | 115 | 2 | 115 | 2.34e-47 | 150 |
| MS.gene046496.t1 | MTR_4g088850 | 58.108 | 74 | 31 | 0 | 44 | 117 | 40 | 113 | 7.78e-26 | 94.7 |
| MS.gene046496.t1 | MTR_4g088850 | 56.000 | 75 | 33 | 0 | 44 | 118 | 40 | 114 | 2.35e-24 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046496.t1 | AT1G32540 | 94.215 | 121 | 7 | 0 | 4 | 124 | 64 | 184 | 8.03e-80 | 233 |
| MS.gene046496.t1 | AT1G32540 | 94.215 | 121 | 7 | 0 | 4 | 124 | 31 | 151 | 3.41e-79 | 230 |
| MS.gene046496.t1 | AT1G32540 | 94.215 | 121 | 7 | 0 | 4 | 124 | 31 | 151 | 3.41e-79 | 230 |
| MS.gene046496.t1 | AT1G32540 | 92.500 | 120 | 9 | 0 | 5 | 124 | 32 | 151 | 2.40e-77 | 226 |
| MS.gene046496.t1 | AT1G32540 | 97.368 | 76 | 2 | 0 | 4 | 79 | 64 | 139 | 9.43e-49 | 153 |
| MS.gene046496.t1 | AT1G32540 | 100.000 | 74 | 0 | 0 | 5 | 78 | 32 | 105 | 2.43e-48 | 150 |
| MS.gene046496.t1 | AT1G32540 | 48.529 | 68 | 34 | 1 | 45 | 111 | 33 | 100 | 1.27e-11 | 57.8 |
| MS.gene046496.t1 | AT1G32540 | 100.000 | 74 | 0 | 0 | 5 | 78 | 32 | 105 | 2.43e-48 | 150 |
| MS.gene046496.t1 | AT1G32540 | 48.529 | 68 | 34 | 1 | 45 | 111 | 33 | 100 | 1.27e-11 | 57.8 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 7 | 130 | 6.13e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 9 | 85 | 2.45e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 7 | 130 | 6.13e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 9 | 85 | 2.45e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 2 | 125 | 7.78e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.54e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 2 | 125 | 7.78e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.54e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 2 | 125 | 7.78e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.54e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 2 | 125 | 7.78e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.54e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.484 | 124 | 38 | 3 | 5 | 117 | 2 | 125 | 7.78e-41 | 134 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.54e-15 | 68.9 |
| MS.gene046496.t1 | AT4G20380 | 60.656 | 122 | 37 | 3 | 5 | 115 | 2 | 123 | 1.39e-40 | 132 |
| MS.gene046496.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 2.12e-15 | 67.8 |
| MS.gene046496.t1 | AT4G20380 | 50.667 | 150 | 37 | 3 | 5 | 117 | 2 | 151 | 1.09e-37 | 127 |
| MS.gene046496.t1 | AT4G20380 | 46.154 | 78 | 39 | 1 | 46 | 120 | 4 | 81 | 1.52e-15 | 70.1 |
| MS.gene046496.t1 | AT4G21610 | 55.294 | 85 | 35 | 1 | 43 | 124 | 57 | 141 | 4.72e-28 | 100 |
| MS.gene046496.t1 | AT4G21610 | 60.274 | 73 | 29 | 0 | 43 | 115 | 57 | 129 | 7.70e-27 | 97.8 |
Find 23 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGTCAACTTGTGTGTTC+TGG | 0.355331 | 8.1:+24453944 | MS.gene046496:CDS |
| ATGCTACTTATGTATCAATA+TGG | 0.369271 | 8.1:+24454488 | MS.gene046496:CDS |
| GTGTATTGTGACAGCAAATC+AGG | 0.393093 | 8.1:+24454436 | MS.gene046496:intron |
| ATTGCAAACTGCACATTTCA+CGG | 0.413666 | 8.1:-24454519 | None:intergenic |
| CATACAGAAGTGGCTCCAAC+TGG | 0.466065 | 8.1:-24453989 | None:intergenic |
| GGATGTACATGAGTAAAGTA+TGG | 0.469461 | 8.1:-24454212 | None:intergenic |
| GTCACAGCAGTGCCACCTCC+TGG | 0.473850 | 8.1:+24454031 | MS.gene046496:CDS |
| GAAATGGCTCAGTTAGTTTG+TGG | 0.475051 | 8.1:+24454183 | MS.gene046496:CDS |
| TGTATTCACAGGCACAGAAA+TGG | 0.481941 | 8.1:+24454167 | MS.gene046496:intron |
| TCACACAGTCAATCTAGCTC+TGG | 0.518089 | 8.1:+24454266 | MS.gene046496:CDS |
| AACCTTCTTATGTATCCAGT+TGG | 0.523909 | 8.1:+24453974 | MS.gene046496:CDS |
| CTCCAACTGGATACATAAGA+AGG | 0.572621 | 8.1:-24453976 | None:intergenic |
| TATTTCATCATACCAGGAGG+TGG | 0.599736 | 8.1:-24454043 | None:intergenic |
| AACAGCACAGCATACAGAAG+TGG | 0.604154 | 8.1:-24453999 | None:intergenic |
| CTGCTCCGTCGTGCTCGCTG+AGG | 0.613383 | 8.1:-24454722 | None:intergenic |
| ATGGCTCAGTTAGTTTGTGG+AGG | 0.620989 | 8.1:+24454186 | MS.gene046496:CDS |
| ACAGTCAATCTAGCTCTGGA+AGG | 0.636792 | 8.1:+24454270 | MS.gene046496:CDS |
| ATGTCAACTGTGGTAACTGC+AGG | 0.637282 | 8.1:+24454465 | MS.gene046496:CDS |
| AACTATACTGACCCCAACTG+AGG | 0.675495 | 8.1:-24454549 | None:intergenic |
| ATTGAACACTCGTCGCTCCG+CGG | 0.692743 | 8.1:-24454233 | None:intergenic |
| CAGGTAGCACATGTCAACTG+TGG | 0.705529 | 8.1:+24454455 | MS.gene046496:CDS |
| ACTTTACTCATGTACATCCG+CGG | 0.709515 | 8.1:+24454216 | MS.gene046496:CDS |
| GCAGGCCTCAGCGAGCACGA+CGG | 0.709773 | 8.1:+24454717 | MS.gene046496:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAAAACTACAAAGTA+CGG | - | chr8.1:24454411-24454430 | None:intergenic | 20.0% |
| ! | TACTCTTCAAAATAGAAAAG+AGG | - | chr8.1:24454596-24454615 | None:intergenic | 25.0% |
| ! | TCAAAATAGAAAAGAGGAAA+AGG | - | chr8.1:24454590-24454609 | None:intergenic | 25.0% |
| ! | TGAATACATCAGGTAAAATA+TGG | - | chr8.1:24454155-24454174 | None:intergenic | 25.0% |
| !! | ATGCTACTTATGTATCAATA+TGG | + | chr8.1:24454488-24454507 | MS.gene046496:CDS | 25.0% |
| AGAGACCTAATAACTGATAA+GGG | - | chr8.1:24454075-24454094 | None:intergenic | 30.0% | |
| ATATCACAAAGTGAAGATTC+AGG | - | chr8.1:24454129-24454148 | None:intergenic | 30.0% | |
| GAAGAATATTTCATCATACC+AGG | - | chr8.1:24454052-24454071 | None:intergenic | 30.0% | |
| TAGAGACCTAATAACTGATA+AGG | - | chr8.1:24454076-24454095 | None:intergenic | 30.0% | |
| ! | ATTTTACCTGATGTATTCAC+AGG | + | chr8.1:24454156-24454175 | MS.gene046496:intron | 30.0% |
| !!! | AAACTATGTCTTGTTTTGCA+AGG | - | chr8.1:24454640-24454659 | None:intergenic | 30.0% |
| AACCTTCTTATGTATCCAGT+TGG | + | chr8.1:24453974-24453993 | MS.gene046496:CDS | 35.0% | |
| ATTGCAAACTGCACATTTCA+CGG | - | chr8.1:24454522-24454541 | None:intergenic | 35.0% | |
| CGAGTTAAATTGCTGTGATT+CGG | + | chr8.1:24454382-24454401 | MS.gene046496:intron | 35.0% | |
| GAAAAGAGGAAAAGGTAGTA+TGG | - | chr8.1:24454582-24454601 | None:intergenic | 35.0% | |
| GAATATTTCATCATACCAGG+AGG | - | chr8.1:24454049-24454068 | None:intergenic | 35.0% | |
| GAGACCTAATAACTGATAAG+GGG | - | chr8.1:24454074-24454093 | None:intergenic | 35.0% | |
| GGATGTACATGAGTAAAGTA+TGG | - | chr8.1:24454215-24454234 | None:intergenic | 35.0% | |
| TCTTCCCCTTATCAGTTATT+AGG | + | chr8.1:24454067-24454086 | MS.gene046496:intron | 35.0% | |
| ! | ATGTTACTGAATGATGATGC+AGG | + | chr8.1:24454699-24454718 | MS.gene046496:intron | 35.0% |
| ! | CAATTTTGTTACCTCAGTTG+GGG | + | chr8.1:24454538-24454557 | MS.gene046496:CDS | 35.0% |
| ! | GCAATTTTGTTACCTCAGTT+GGG | + | chr8.1:24454537-24454556 | MS.gene046496:CDS | 35.0% |
| ! | TGCAATTTTGTTACCTCAGT+TGG | + | chr8.1:24454536-24454555 | MS.gene046496:CDS | 35.0% |
| ACTTTACTCATGTACATCCG+CGG | + | chr8.1:24454216-24454235 | MS.gene046496:CDS | 40.0% | |
| CTCCAACTGGATACATAAGA+AGG | - | chr8.1:24453979-24453998 | None:intergenic | 40.0% | |
| GTGTATTGTGACAGCAAATC+AGG | + | chr8.1:24454436-24454455 | MS.gene046496:intron | 40.0% | |
| TATTTCATCATACCAGGAGG+TGG | - | chr8.1:24454046-24454065 | None:intergenic | 40.0% | |
| TGTATTCACAGGCACAGAAA+TGG | + | chr8.1:24454167-24454186 | MS.gene046496:intron | 40.0% | |
| ! | GAAATGGCTCAGTTAGTTTG+TGG | + | chr8.1:24454183-24454202 | MS.gene046496:CDS | 40.0% |
| AACAGCACAGCATACAGAAG+TGG | - | chr8.1:24454002-24454021 | None:intergenic | 45.0% | |
| AACTATACTGACCCCAACTG+AGG | - | chr8.1:24454552-24454571 | None:intergenic | 45.0% | |
| ATGTCAACTGTGGTAACTGC+AGG | + | chr8.1:24454465-24454484 | MS.gene046496:CDS | 45.0% | |
| CAGAGTCAACTTGTGTGTTC+TGG | + | chr8.1:24453944-24453963 | MS.gene046496:CDS | 45.0% | |
| TCTGTGCCTGTGAATACATC+AGG | - | chr8.1:24454165-24454184 | None:intergenic | 45.0% | |
| ! | TCACACAGTCAATCTAGCTC+TGG | + | chr8.1:24454266-24454285 | MS.gene046496:CDS | 45.0% |
| !! | ACAGTCAATCTAGCTCTGGA+AGG | + | chr8.1:24454270-24454289 | MS.gene046496:CDS | 45.0% |
| !! | ATGGCTCAGTTAGTTTGTGG+AGG | + | chr8.1:24454186-24454205 | MS.gene046496:CDS | 45.0% |
| CAGGTAGCACATGTCAACTG+TGG | + | chr8.1:24454455-24454474 | MS.gene046496:CDS | 50.0% | |
| CATACAGAAGTGGCTCCAAC+TGG | - | chr8.1:24453992-24454011 | None:intergenic | 50.0% | |
| ATTGAACACTCGTCGCTCCG+CGG | - | chr8.1:24454236-24454255 | None:intergenic | 55.0% | |
| GTCACAGCAGTGCCACCTCC+TGG | + | chr8.1:24454031-24454050 | MS.gene046496:CDS | 65.0% | |
| CTGCTCCGTCGTGCTCGCTG+AGG | - | chr8.1:24454725-24454744 | None:intergenic | 70.0% | |
| GCAGGCCTCAGCGAGCACGA+CGG | + | chr8.1:24454717-24454736 | MS.gene046496:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 24453932 | 24454762 | 24453932 | ID=MS.gene046496 |
| chr8.1 | mRNA | 24453932 | 24454762 | 24453932 | ID=MS.gene046496.t1;Parent=MS.gene046496 |
| chr8.1 | exon | 24453932 | 24454052 | 24453932 | ID=MS.gene046496.t1.exon1;Parent=MS.gene046496.t1 |
| chr8.1 | CDS | 24453932 | 24454052 | 24453932 | ID=cds.MS.gene046496.t1;Parent=MS.gene046496.t1 |
| chr8.1 | exon | 24454178 | 24454291 | 24454178 | ID=MS.gene046496.t1.exon2;Parent=MS.gene046496.t1 |
| chr8.1 | CDS | 24454178 | 24454291 | 24454178 | ID=cds.MS.gene046496.t1;Parent=MS.gene046496.t1 |
| chr8.1 | exon | 24454450 | 24454559 | 24454450 | ID=MS.gene046496.t1.exon3;Parent=MS.gene046496.t1 |
| chr8.1 | CDS | 24454450 | 24454559 | 24454450 | ID=cds.MS.gene046496.t1;Parent=MS.gene046496.t1 |
| chr8.1 | exon | 24454721 | 24454762 | 24454721 | ID=MS.gene046496.t1.exon4;Parent=MS.gene046496.t1 |
| chr8.1 | CDS | 24454721 | 24454762 | 24454721 | ID=cds.MS.gene046496.t1;Parent=MS.gene046496.t1 |
| Gene Sequence |
| Protein sequence |
