AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047186

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047186.t1 MTR_2g006380 88.820 322 27 5 1 317 1 318 0.0 565
MS.gene047186.t1 MTR_2g006270 72.642 318 72 7 10 317 4 316 5.04e-156 444
MS.gene047186.t1 MTR_1g058210 48.607 323 140 11 12 317 5 318 6.38e-86 266
MS.gene047186.t1 MTR_5g040880 43.711 318 161 8 10 317 2 311 3.92e-82 258
MS.gene047186.t1 MTR_2g024430 47.500 320 143 11 12 317 13 321 1.34e-81 256
MS.gene047186.t1 MTR_5g061220 45.665 346 136 12 13 317 13 347 1.44e-80 254
MS.gene047186.t1 MTR_5g040740 46.875 320 148 11 11 317 4 314 9.84e-80 248
MS.gene047186.t1 MTR_5g060780 41.718 326 153 12 13 317 6 315 5.21e-72 228
MS.gene047186.t1 MTR_5g061890 42.154 325 153 12 13 317 6 315 3.46e-71 226
MS.gene047186.t1 MTR_5g460920 41.718 326 153 12 13 317 6 315 4.07e-71 226
MS.gene047186.t1 MTR_5g082140 44.062 320 159 10 11 317 4 316 1.82e-70 226
MS.gene047186.t1 MTR_5g060630 42.045 352 152 12 12 317 5 350 3.29e-70 227
MS.gene047186.t1 MTR_5g460740 42.045 352 152 12 12 317 5 350 3.29e-70 227
MS.gene047186.t1 MTR_3g073420 39.385 358 152 12 14 317 14 360 5.65e-69 225
MS.gene047186.t1 MTR_2g094570 33.598 378 176 13 3 317 46 411 5.15e-56 194
MS.gene047186.t1 MTR_2g094570 34.167 360 167 12 16 317 8 355 7.04e-56 192
MS.gene047186.t1 MTR_2g094570 34.167 360 167 12 16 317 8 355 7.19e-56 192
MS.gene047186.t1 MTR_5g046120 80.556 108 19 1 65 170 16 123 3.67e-55 176
MS.gene047186.t1 MTR_1g064430 33.151 365 172 11 16 317 24 379 1.00e-53 186
MS.gene047186.t1 MTR_1g064430 33.151 365 172 11 16 317 37 392 1.25e-53 186
MS.gene047186.t1 MTR_1g094960 33.708 356 167 11 19 317 19 362 4.57e-51 178
MS.gene047186.t1 MTR_7g101275 36.039 308 168 10 29 316 2 300 7.44e-49 168
MS.gene047186.t1 MTR_5g062970 45.631 206 98 5 19 219 38 234 6.16e-48 161
MS.gene047186.t1 MTR_4g058930 32.000 375 175 14 9 317 2 362 1.47e-44 161
MS.gene047186.t1 MTR_4g058930 32.000 375 175 14 9 317 2 362 1.66e-44 161
MS.gene047186.t1 MTR_0041s0120 32.609 322 172 10 29 316 2 312 4.74e-42 150
MS.gene047186.t1 MTR_7g101280 36.792 212 122 4 16 223 28 231 5.49e-37 140
MS.gene047186.t1 MTR_0041s0110 36.792 212 122 4 16 223 28 231 5.49e-37 140
MS.gene047186.t1 MTR_4g124900 31.498 327 188 12 16 317 20 335 1.26e-35 137
MS.gene047186.t1 MTR_4g124900 31.498 327 188 12 16 317 20 335 1.40e-35 137
MS.gene047186.t1 MTR_8g101360 30.887 327 192 11 16 317 28 345 1.75e-33 130
MS.gene047186.t1 MTR_5g060770 38.095 252 105 9 74 316 9 218 2.25e-33 126
MS.gene047186.t1 MTR_1g024025 29.755 326 196 11 16 317 40 356 3.28e-33 130
MS.gene047186.t1 MTR_2g043250 30.031 323 198 10 16 317 20 335 3.66e-33 129
MS.gene047186.t1 MTR_2g014770 28.617 311 186 10 19 306 53 350 7.82e-33 128
MS.gene047186.t1 MTR_8g079492 30.408 319 197 9 19 317 23 336 8.81e-33 128
MS.gene047186.t1 MTR_8g079492 30.408 319 197 9 19 317 23 336 9.88e-33 128
MS.gene047186.t1 MTR_2g014770 28.617 311 186 10 19 306 53 350 1.37e-32 127
MS.gene047186.t1 MTR_4g021580 30.124 322 191 12 19 317 19 329 1.44e-32 127
MS.gene047186.t1 MTR_2g018690 30.565 301 191 8 19 305 23 319 1.60e-32 127
MS.gene047186.t1 MTR_4g021580 30.124 322 191 12 19 317 19 329 1.87e-32 127
MS.gene047186.t1 MTR_2g093740 31.003 329 191 12 19 317 56 378 2.06e-32 127
MS.gene047186.t1 MTR_5g076270 29.283 321 205 10 2 306 7 321 2.18e-32 127
MS.gene047186.t1 MTR_2g093740 31.003 329 191 12 19 317 56 378 2.45e-32 127
MS.gene047186.t1 MTR_2g093740 31.003 329 191 12 19 317 56 378 2.89e-32 127
MS.gene047186.t1 MTR_2g005240 29.938 324 196 11 16 317 20 334 3.25e-32 126
MS.gene047186.t1 MTR_8g100050 29.412 323 194 11 19 317 59 371 7.03e-32 125
MS.gene047186.t1 MTR_8g100050 29.412 323 194 11 19 317 59 371 1.10e-31 125
MS.gene047186.t1 MTR_3g064050 29.808 312 187 11 16 306 23 323 1.22e-31 125
MS.gene047186.t1 MTR_2g093740 29.825 342 191 12 19 317 56 391 1.77e-30 121
MS.gene047186.t1 MTR_2g093740 29.825 342 191 12 19 317 56 391 1.85e-30 121
MS.gene047186.t1 MTR_2g093740 29.825 342 191 12 19 317 56 391 2.36e-30 121
MS.gene047186.t1 MTR_7g062540 30.061 326 192 12 16 317 11 324 2.09e-29 118
MS.gene047186.t1 MTR_8g027440 28.981 314 195 10 16 312 26 328 2.40e-29 118
MS.gene047186.t1 MTR_4g088210 29.934 304 170 10 14 289 33 321 5.04e-29 117
MS.gene047186.t1 MTR_4g088210 29.934 304 170 10 14 289 33 321 5.11e-29 117
MS.gene047186.t1 MTR_4g060460 30.333 300 176 10 19 293 54 345 2.50e-26 109
MS.gene047186.t1 MTR_4g060460 30.333 300 176 10 19 293 54 345 3.31e-26 109
MS.gene047186.t1 MTR_8g079492 30.588 255 154 8 83 317 3 254 1.10e-23 102
MS.gene047186.t1 MTR_2g093740 30.522 249 135 11 105 317 2 248 3.59e-18 85.5
MS.gene047186.t1 MTR_2g093740 30.522 249 135 11 105 317 2 248 3.84e-18 85.5
MS.gene047186.t1 MTR_5g460910 30.712 267 111 10 59 316 12 213 3.98e-18 84.0
MS.gene047186.t1 MTR_8g446900 26.316 247 156 9 73 302 13 250 2.52e-16 79.3
MS.gene047186.t1 MTR_2g093740 30.088 226 122 9 126 317 38 261 2.61e-16 80.1
MS.gene047186.t1 MTR_2g093740 29.008 262 135 11 105 317 2 261 2.69e-16 79.7
MS.gene047186.t1 MTR_5g062960 47.191 89 36 5 239 317 1 88 4.15e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047186.t1 AT1G77850 36.232 345 163 10 15 313 15 348 1.66e-60 203
MS.gene047186.t1 AT1G77850 36.232 345 163 10 15 313 15 348 1.78e-60 203
MS.gene047186.t1 AT4G30080 34.078 358 170 10 16 317 16 363 3.22e-53 185
MS.gene047186.t1 AT2G28350 32.615 371 175 13 11 317 2 361 5.24e-49 173
MS.gene047186.t1 AT2G28350 32.615 371 175 13 11 317 2 361 7.23e-49 173
MS.gene047186.t1 AT4G23980 30.435 322 198 10 15 317 8 322 4.95e-37 140
MS.gene047186.t1 AT4G23980 30.435 322 198 10 15 317 8 322 5.01e-37 140
MS.gene047186.t1 AT2G33860 32.622 328 179 13 19 317 54 368 5.64e-36 137
MS.gene047186.t1 AT1G35540 31.692 325 187 12 14 317 22 332 7.44e-36 136
MS.gene047186.t1 AT1G34310 31.173 324 190 11 14 317 22 332 4.01e-35 134
MS.gene047186.t1 AT1G34390 30.476 315 189 10 14 313 22 321 4.49e-35 134
MS.gene047186.t1 AT1G35520 31.111 315 189 10 14 313 22 323 1.32e-34 133
MS.gene047186.t1 AT5G37020 29.204 339 208 12 2 317 7 336 1.25e-33 130
MS.gene047186.t1 AT5G37020 29.204 339 208 12 2 317 7 336 1.33e-33 130
MS.gene047186.t1 AT5G20730 30.488 328 193 11 15 317 20 337 2.28e-33 130
MS.gene047186.t1 AT5G20730 30.488 328 193 11 15 317 20 337 2.52e-33 130
MS.gene047186.t1 AT1G35240 30.247 324 193 11 14 317 22 332 8.07e-33 128
MS.gene047186.t1 AT5G20730 30.488 328 192 12 15 317 20 336 1.05e-32 128
MS.gene047186.t1 AT1G30330 30.275 327 194 11 16 317 20 337 1.24e-31 125
MS.gene047186.t1 AT1G30330 30.275 327 194 11 16 317 22 339 1.38e-31 125
MS.gene047186.t1 AT1G30330 30.275 327 194 11 16 317 22 339 1.38e-31 125
MS.gene047186.t1 AT1G19220 29.141 326 197 11 16 317 20 335 1.60e-31 125
MS.gene047186.t1 AT1G34410 29.938 324 194 11 14 317 22 332 2.56e-31 124
MS.gene047186.t1 AT2G46530 28.571 322 203 10 15 317 38 351 3.13e-31 123
MS.gene047186.t1 AT2G46530 28.571 322 203 10 15 317 17 330 3.51e-31 123
MS.gene047186.t1 AT3G61830 29.448 326 195 12 15 317 21 334 4.77e-31 123
MS.gene047186.t1 AT1G19850 29.480 346 201 12 1 317 37 368 6.08e-31 123
MS.gene047186.t1 AT1G19850 29.480 346 201 12 1 317 26 357 6.41e-31 123
MS.gene047186.t1 AT5G62000 28.000 325 202 10 16 317 58 373 9.74e-31 122
MS.gene047186.t1 AT5G62000 28.000 325 202 10 16 317 58 373 1.32e-30 122
MS.gene047186.t1 AT5G62000 28.000 325 202 10 16 317 58 373 1.32e-30 122
MS.gene047186.t1 AT5G62000 28.000 325 202 10 16 317 58 373 1.32e-30 122
MS.gene047186.t1 AT5G62000 28.000 325 202 10 16 317 58 373 1.32e-30 122
MS.gene047186.t1 AT5G60450 28.916 332 202 10 14 317 36 361 3.01e-30 121
MS.gene047186.t1 AT5G60450 28.788 330 201 10 16 317 62 385 8.53e-30 119
MS.gene047186.t1 AT1G59750 27.795 331 188 12 19 317 22 333 5.00e-28 114
MS.gene047186.t1 AT1G59750 27.795 331 188 12 19 317 22 333 5.95e-28 114
MS.gene047186.t1 AT1G59750 27.795 331 188 12 19 317 22 333 6.33e-28 114
MS.gene047186.t1 AT1G59750 27.795 331 188 12 19 317 22 333 6.33e-28 114
MS.gene047186.t1 AT1G34170 28.391 317 197 12 14 313 20 323 9.56e-28 113
MS.gene047186.t1 AT1G34170 28.383 303 169 12 14 281 20 309 1.95e-27 112
MS.gene047186.t1 AT1G43950 32.110 218 122 7 14 222 22 222 2.22e-27 107
MS.gene047186.t1 AT1G34170 28.383 303 169 12 14 281 20 309 3.73e-27 111
MS.gene047186.t1 AT2G33860 31.776 214 120 8 126 317 2 211 4.50e-21 93.6
MS.gene047186.t1 AT3G61830 28.839 267 164 9 70 317 24 283 1.42e-20 92.4
MS.gene047186.t1 AT5G37020 30.047 213 126 8 126 317 31 241 7.41e-20 90.5
MS.gene047186.t1 AT2G46530 29.302 215 129 8 122 317 33 243 2.10e-15 77.0

Find 88 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATGCAACTTTGGTATT+TGG 0.133681 2.2:+73297691 None:intergenic
TTCATCTTAACTCTCATTCT+AGG 0.234993 2.2:+73296934 None:intergenic
TATCTCGTACGTCTAATTTA+TGG 0.240815 2.2:-73296864 MS.gene047186:CDS
CTCATCATCGTCGTTGCAAT+TGG 0.314881 2.2:+73297437 None:intergenic
GTAGAGATTGTCCCTTGATA+TGG 0.324016 2.2:+73296889 None:intergenic
AACGACGATGATGAGGTAAT+TGG 0.328138 2.2:-73297429 MS.gene047186:CDS
GCTGCTGTTGGTGCTGGTGC+TGG 0.335243 2.2:+73297766 None:intergenic
GTATGCTTGCTTACATTAGT+AGG 0.336336 2.2:+73297384 None:intergenic
GCATCAACATCATTGGTTAT+TGG 0.339316 2.2:+73297486 None:intergenic
TTAACAAACGAACTCCATTC+AGG 0.339363 2.2:+73297210 None:intergenic
CGCATGTCCACCCTCAAATC+TGG 0.363208 2.2:-73297737 MS.gene047186:CDS
CAACGGGAAAATTATTTGTT+GGG 0.375166 2.2:-73297140 MS.gene047186:CDS
ATGGTTGCTGCTGTTGGTGC+TGG 0.385990 2.2:+73297760 None:intergenic
CAAATCTGGCAGACATTAAC+CGG 0.388348 2.2:-73297723 MS.gene047186:CDS
TAAACTCTTGAATGCAACTT+TGG 0.402834 2.2:+73297684 None:intergenic
TCAACGGGAAAATTATTTGT+TGG 0.404341 2.2:-73297141 MS.gene047186:CDS
CTGACGTTCATGGTGAAGTC+TGG 0.406535 2.2:-73297284 MS.gene047186:CDS
CTCCTCGTCACTGACGTTCA+TGG 0.407231 2.2:-73297294 MS.gene047186:CDS
AATGGAGTTCGTTTGTTAAG+AGG 0.407313 2.2:-73297206 MS.gene047186:CDS
TAATTTATGGCGCATGCTAC+AGG 0.422222 2.2:-73296851 MS.gene047186:CDS
TGAGTTGGAGAAGGCAGTAA+TGG 0.441158 2.2:-73297082 MS.gene047186:CDS
ACTATCCTCGAGGTGATGAT+TGG 0.444363 2.2:-73297014 MS.gene047186:CDS
GACACAGGCATCAACATCAT+TGG 0.444932 2.2:+73297479 None:intergenic
GTTAATGTCTGCCAGATTTG+AGG 0.445255 2.2:+73297726 None:intergenic
TCATCTTAACTCTCATTCTA+GGG 0.445548 2.2:+73296935 None:intergenic
GATATAAAACACGTTTCTCT+TGG 0.446560 2.2:+73297233 None:intergenic
AGGTAATTGGTTCGTTTACA+AGG 0.453827 2.2:-73297416 MS.gene047186:CDS
TGAAGACTGATAAATCTTCA+AGG 0.454632 2.2:-73296915 MS.gene047186:CDS
TGGGAACCTCATGAGGGTCT+TGG 0.457592 2.2:+73297457 None:intergenic
AATTATTTGTTGGGATTCGC+AGG 0.462606 2.2:-73297131 MS.gene047186:CDS
GGATAGTAAACAATTTCAAA+TGG 0.472676 2.2:+73297030 None:intergenic
AGTAATGGAGGCAGTGAAGT+TGG 0.472794 2.2:-73297067 MS.gene047186:CDS
TTAATGTCTGCCAGATTTGA+GGG 0.479203 2.2:+73297727 None:intergenic
ATGAATCAATGAAAATTCAA+TGG 0.482116 2.2:-73296960 MS.gene047186:CDS
GGAGTGAATGTAAGCATTGG+AGG 0.486006 2.2:+73297617 None:intergenic
TCTCTTGGCAAACCCGTGGC+AGG 0.490609 2.2:+73297248 None:intergenic
TCATCATCGTCGTTGCAATT+GGG 0.492508 2.2:+73297438 None:intergenic
GAAAATATCTTCCCTCCGCT+TGG 0.492876 2.2:-73297336 MS.gene047186:CDS
TAGAGATTGTCCCTTGATAT+GGG 0.494859 2.2:+73296890 None:intergenic
CTCATGAGGGTCTTGGACAC+AGG 0.498403 2.2:+73297464 None:intergenic
TATGCTTGCTTACATTAGTA+GGG 0.498565 2.2:+73297385 None:intergenic
TGGTATTTGGACAGCAGTGC+CGG 0.499421 2.2:+73297704 None:intergenic
TGGCAAACCCGTGGCAGGCA+TGG 0.500790 2.2:+73297253 None:intergenic
AAGTTTCACCATGCCTGCCA+CGG 0.506565 2.2:-73297261 MS.gene047186:CDS
TAACAAACGAACTCCATTCA+GGG 0.512751 2.2:+73297211 None:intergenic
TCTTCAAGGATCCCATATCA+AGG 0.512818 2.2:-73296901 MS.gene047186:CDS
AGTTTCACCATGCCTGCCAC+GGG 0.517459 2.2:-73297260 MS.gene047186:CDS
ATGTAAGCATTGGAGGAGGA+AGG 0.526102 2.2:+73297624 None:intergenic
CATGCGATGGTTGCTGCTGT+TGG 0.533873 2.2:+73297754 None:intergenic
TTGCAATTGGGAACCTCATG+AGG 0.534875 2.2:+73297450 None:intergenic
TAAGCATTGGAGGAGGAAGG+AGG 0.538830 2.2:+73297627 None:intergenic
GGAGGCAGTGAAGTTGGCAG+AGG 0.547497 2.2:-73297061 MS.gene047186:CDS
TATCTTCCCTCCGCTTGGCA+TGG 0.558368 2.2:-73297331 MS.gene047186:CDS
CGCCGATCCTCACACTGATG+AGG 0.560935 2.2:-73297532 MS.gene047186:CDS
AAGGGAGTGAATGTAAGCAT+TGG 0.561164 2.2:+73297614 None:intergenic
CTGGTGCTGGTTGAGAGACA+TGG 0.567756 2.2:+73297779 None:intergenic
GCATATTCTAAGTGGCCTTG+TGG 0.574209 2.2:+73297654 None:intergenic
GTGAATGTAAGCATTGGAGG+AGG 0.588009 2.2:+73297620 None:intergenic
GTTGGAGAAGGCAGTAATGG+AGG 0.592416 2.2:-73297079 MS.gene047186:CDS
TGGGATTCGCAGGAAAGCCG+CGG 0.593939 2.2:-73297121 MS.gene047186:CDS
GGAGCAAAAGAAAGATGAGT+TGG 0.594103 2.2:-73297097 MS.gene047186:CDS
GACCTCATCAGTGTGAGGAT+CGG 0.595759 2.2:+73297530 None:intergenic
AAAGAAAGATGAGTTGGAGA+AGG 0.605720 2.2:-73297091 MS.gene047186:CDS
AAATCTCCATGCCAAGCGGA+GGG 0.609417 2.2:+73297325 None:intergenic
CTTCAAGGATCCCATATCAA+GGG 0.609531 2.2:-73296900 MS.gene047186:CDS
GAGATGATACAGATAGTGAA+AGG 0.609814 2.2:+73297570 None:intergenic
GAAATCTCCATGCCAAGCGG+AGG 0.617181 2.2:+73297324 None:intergenic
CACCATGAACGTCAGTGACG+AGG 0.617900 2.2:+73297292 None:intergenic
CGTTTCTCTTGGCAAACCCG+TGG 0.618791 2.2:+73297244 None:intergenic
AAAACGTTGAAGATCATGAA+GGG 0.620045 2.2:+73297596 None:intergenic
CACTATCTGTATCATCTCCG+CGG 0.622122 2.2:-73297565 MS.gene047186:CDS
GAGGAGAAGCATATTCTAAG+TGG 0.626940 2.2:+73297646 None:intergenic
GAAAACGTTGAAGATCATGA+AGG 0.628457 2.2:+73297595 None:intergenic
CAATTGCAACGACGATGATG+AGG 0.630770 2.2:-73297436 MS.gene047186:CDS
GAAATTGTTTACTATCCTCG+AGG 0.641156 2.2:-73297024 MS.gene047186:CDS
TGCAATTGGGAACCTCATGA+GGG 0.643730 2.2:+73297451 None:intergenic
ATTTGAGGGTGGACATGCGA+TGG 0.643879 2.2:+73297741 None:intergenic
ATGCTTGCTTACATTAGTAG+GGG 0.652528 2.2:+73297386 None:intergenic
CATGAACGTCAGTGACGAGG+AGG 0.677277 2.2:+73297295 None:intergenic
GCGAAGACCTCATCAGTGTG+AGG 0.682334 2.2:+73297525 None:intergenic
ATGTCTGCCAGATTTGAGGG+TGG 0.682807 2.2:+73297730 None:intergenic
ATCGGCGAGGAGATCAACCG+CGG 0.684227 2.2:+73297548 None:intergenic
TGAGAAATCTCCATGCCAAG+CGG 0.686661 2.2:+73297321 None:intergenic
CTGTGTCCAAGACCCTCATG+AGG 0.688345 2.2:-73297463 MS.gene047186:CDS
GATTCGCAGGAAAGCCGCGG+CGG 0.692782 2.2:-73297118 MS.gene047186:CDS
TCATGCCAATCATCACCTCG+AGG 0.713899 2.2:+73297009 None:intergenic
AAGAGGAAGAAACTCGACGT+CGG 0.718798 2.2:-73297189 MS.gene047186:CDS
CATCAGTGTGAGGATCGGCG+AGG 0.752555 2.2:+73297535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATCAATGAAAATTCAA+TGG - chr2.2:73297663-73297682 MS.gene047186:CDS 20.0%
! GGATAGTAAACAATTTCAAA+TGG + chr2.2:73297596-73297615 None:intergenic 25.0%
!! TGTTTTCATGAAAAACTCAA+CGG - chr2.2:73297466-73297485 MS.gene047186:CDS 25.0%
AAAACGTTGAAGATCATGAA+GGG + chr2.2:73297030-73297049 None:intergenic 30.0%
CAACGGGAAAATTATTTGTT+GGG - chr2.2:73297483-73297502 MS.gene047186:CDS 30.0%
GATATAAAACACGTTTCTCT+TGG + chr2.2:73297393-73297412 None:intergenic 30.0%
TAAACTCTTGAATGCAACTT+TGG + chr2.2:73296942-73296961 None:intergenic 30.0%
TATCTCGTACGTCTAATTTA+TGG - chr2.2:73297759-73297778 MS.gene047186:CDS 30.0%
TATGCTTGCTTACATTAGTA+GGG + chr2.2:73297241-73297260 None:intergenic 30.0%
TCAACGGGAAAATTATTTGT+TGG - chr2.2:73297482-73297501 MS.gene047186:CDS 30.0%
TCATCTTAACTCTCATTCTA+GGG + chr2.2:73297691-73297710 None:intergenic 30.0%
TGAAGACTGATAAATCTTCA+AGG - chr2.2:73297708-73297727 MS.gene047186:CDS 30.0%
TTCATCTTAACTCTCATTCT+AGG + chr2.2:73297692-73297711 None:intergenic 30.0%
TTGAATGCAACTTTGGTATT+TGG + chr2.2:73296935-73296954 None:intergenic 30.0%
! AGAGTTTACTATTTTCCACA+AGG - chr2.2:73296954-73296973 MS.gene047186:CDS 30.0%
! AGCATACATTTTACATACCT+CGG - chr2.2:73297255-73297274 MS.gene047186:CDS 30.0%
! GTTTTCATGAAAAACTCAAC+GGG - chr2.2:73297467-73297486 MS.gene047186:CDS 30.0%
ATGCTTGCTTACATTAGTAG+GGG + chr2.2:73297240-73297259 None:intergenic 35.0%
GAAAACGTTGAAGATCATGA+AGG + chr2.2:73297031-73297050 None:intergenic 35.0%
GAAATTGTTTACTATCCTCG+AGG - chr2.2:73297599-73297618 MS.gene047186:CDS 35.0%
GAGATGATACAGATAGTGAA+AGG + chr2.2:73297056-73297075 None:intergenic 35.0%
GTATGCTTGCTTACATTAGT+AGG + chr2.2:73297242-73297261 None:intergenic 35.0%
TAACAAACGAACTCCATTCA+GGG + chr2.2:73297415-73297434 None:intergenic 35.0%
TAGAGATTGTCCCTTGATAT+GGG + chr2.2:73297736-73297755 None:intergenic 35.0%
TTAACAAACGAACTCCATTC+AGG + chr2.2:73297416-73297435 None:intergenic 35.0%
TTAATGTCTGCCAGATTTGA+GGG + chr2.2:73296899-73296918 None:intergenic 35.0%
! AAAGAAAGATGAGTTGGAGA+AGG - chr2.2:73297532-73297551 MS.gene047186:CDS 35.0%
! AATGGAGTTCGTTTGTTAAG+AGG - chr2.2:73297417-73297436 MS.gene047186:CDS 35.0%
! AATTATTTGTTGGGATTCGC+AGG - chr2.2:73297492-73297511 MS.gene047186:CDS 35.0%
! ACGTGTTTTATATCCCTGAA+TGG - chr2.2:73297399-73297418 MS.gene047186:CDS 35.0%
! ATATTTTCAGCACAAAACCG+AGG + chr2.2:73297275-73297294 None:intergenic 35.0%
! GCATCAACATCATTGGTTAT+TGG + chr2.2:73297140-73297159 None:intergenic 35.0%
! TGATTGGCATGATTTTGTAG+TGG - chr2.2:73297625-73297644 MS.gene047186:CDS 35.0%
!! AGGTAATTGGTTCGTTTACA+AGG - chr2.2:73297207-73297226 MS.gene047186:CDS 35.0%
AACGACGATGATGAGGTAAT+TGG - chr2.2:73297194-73297213 MS.gene047186:CDS 40.0%
AAGGGAGTGAATGTAAGCAT+TGG + chr2.2:73297012-73297031 None:intergenic 40.0%
CAAATCTGGCAGACATTAAC+CGG - chr2.2:73296900-73296919 MS.gene047186:CDS 40.0%
CTTCAAGGATCCCATATCAA+GGG - chr2.2:73297723-73297742 MS.gene047186:CDS 40.0%
GGAGCAAAAGAAAGATGAGT+TGG - chr2.2:73297526-73297545 MS.gene047186:CDS 40.0%
GTAGAGATTGTCCCTTGATA+TGG + chr2.2:73297737-73297756 None:intergenic 40.0%
GTTAATGTCTGCCAGATTTG+AGG + chr2.2:73296900-73296919 None:intergenic 40.0%
TAATTTATGGCGCATGCTAC+AGG - chr2.2:73297772-73297791 MS.gene047186:CDS 40.0%
TCATCATCGTCGTTGCAATT+GGG + chr2.2:73297188-73297207 None:intergenic 40.0%
TCTTCAAGGATCCCATATCA+AGG - chr2.2:73297722-73297741 MS.gene047186:CDS 40.0%
! GAGGAGAAGCATATTCTAAG+TGG + chr2.2:73296980-73296999 None:intergenic 40.0%
AAGAGGAAGAAACTCGACGT+CGG - chr2.2:73297434-73297453 MS.gene047186:CDS 45.0%
ACTATCCTCGAGGTGATGAT+TGG - chr2.2:73297609-73297628 MS.gene047186:CDS 45.0%
CAATTGCAACGACGATGATG+AGG - chr2.2:73297187-73297206 MS.gene047186:CDS 45.0%
CACTATCTGTATCATCTCCG+CGG - chr2.2:73297058-73297077 MS.gene047186:CDS 45.0%
CTCATCATCGTCGTTGCAAT+TGG + chr2.2:73297189-73297208 None:intergenic 45.0%
GAAAATATCTTCCCTCCGCT+TGG - chr2.2:73297287-73297306 MS.gene047186:CDS 45.0%
GACACAGGCATCAACATCAT+TGG + chr2.2:73297147-73297166 None:intergenic 45.0%
TGAGAAATCTCCATGCCAAG+CGG + chr2.2:73297305-73297324 None:intergenic 45.0%
TGCAATTGGGAACCTCATGA+GGG + chr2.2:73297175-73297194 None:intergenic 45.0%
TTGCAATTGGGAACCTCATG+AGG + chr2.2:73297176-73297195 None:intergenic 45.0%
! AGTAATGGAGGCAGTGAAGT+TGG - chr2.2:73297556-73297575 MS.gene047186:CDS 45.0%
! GCATATTCTAAGTGGCCTTG+TGG + chr2.2:73296972-73296991 None:intergenic 45.0%
! TGAGTTGGAGAAGGCAGTAA+TGG - chr2.2:73297541-73297560 MS.gene047186:CDS 45.0%
!! ATGTAAGCATTGGAGGAGGA+AGG + chr2.2:73297002-73297021 None:intergenic 45.0%
!! GGAGTGAATGTAAGCATTGG+AGG + chr2.2:73297009-73297028 None:intergenic 45.0%
!! GTGAATGTAAGCATTGGAGG+AGG + chr2.2:73297006-73297025 None:intergenic 45.0%
AAATCTCCATGCCAAGCGGA+GGG + chr2.2:73297301-73297320 None:intergenic 50.0%
AAGTTTCACCATGCCTGCCA+CGG - chr2.2:73297362-73297381 MS.gene047186:CDS 50.0%
ATGTCTGCCAGATTTGAGGG+TGG + chr2.2:73296896-73296915 None:intergenic 50.0%
ATTTGAGGGTGGACATGCGA+TGG + chr2.2:73296885-73296904 None:intergenic 50.0%
CTGACGTTCATGGTGAAGTC+TGG - chr2.2:73297339-73297358 MS.gene047186:CDS 50.0%
GACCTCATCAGTGTGAGGAT+CGG + chr2.2:73297096-73297115 None:intergenic 50.0%
GTTGGAGAAGGCAGTAATGG+AGG - chr2.2:73297544-73297563 MS.gene047186:CDS 50.0%
TCATGCCAATCATCACCTCG+AGG + chr2.2:73297617-73297636 None:intergenic 50.0%
TGGTATTTGGACAGCAGTGC+CGG + chr2.2:73296922-73296941 None:intergenic 50.0%
! ATCTTTCTTTTGCTCCGCCG+CGG + chr2.2:73297522-73297541 None:intergenic 50.0%
!! TAAGCATTGGAGGAGGAAGG+AGG + chr2.2:73296999-73297018 None:intergenic 50.0%
AGTTTCACCATGCCTGCCAC+GGG - chr2.2:73297363-73297382 MS.gene047186:CDS 55.0%
ATGGTTGCTGCTGTTGGTGC+TGG + chr2.2:73296866-73296885 None:intergenic 55.0%
CACCATGAACGTCAGTGACG+AGG + chr2.2:73297334-73297353 None:intergenic 55.0%
CATGAACGTCAGTGACGAGG+AGG + chr2.2:73297331-73297350 None:intergenic 55.0%
CATGCGATGGTTGCTGCTGT+TGG + chr2.2:73296872-73296891 None:intergenic 55.0%
CGCATGTCCACCCTCAAATC+TGG - chr2.2:73296886-73296905 MS.gene047186:CDS 55.0%
CGTTTCTCTTGGCAAACCCG+TGG + chr2.2:73297382-73297401 None:intergenic 55.0%
CTCATGAGGGTCTTGGACAC+AGG + chr2.2:73297162-73297181 None:intergenic 55.0%
CTCCTCGTCACTGACGTTCA+TGG - chr2.2:73297329-73297348 MS.gene047186:CDS 55.0%
CTGTGTCCAAGACCCTCATG+AGG - chr2.2:73297160-73297179 MS.gene047186:CDS 55.0%
GAAATCTCCATGCCAAGCGG+AGG + chr2.2:73297302-73297321 None:intergenic 55.0%
GCGAAGACCTCATCAGTGTG+AGG + chr2.2:73297101-73297120 None:intergenic 55.0%
TATCTTCCCTCCGCTTGGCA+TGG - chr2.2:73297292-73297311 MS.gene047186:CDS 55.0%
TGGGAACCTCATGAGGGTCT+TGG + chr2.2:73297169-73297188 None:intergenic 55.0%
ATCGGCGAGGAGATCAACCG+CGG + chr2.2:73297078-73297097 None:intergenic 60.0%
CATCAGTGTGAGGATCGGCG+AGG + chr2.2:73297091-73297110 None:intergenic 60.0%
CGCCGATCCTCACACTGATG+AGG - chr2.2:73297091-73297110 MS.gene047186:CDS 60.0%
GGAGGCAGTGAAGTTGGCAG+AGG - chr2.2:73297562-73297581 MS.gene047186:CDS 60.0%
TCTCTTGGCAAACCCGTGGC+AGG + chr2.2:73297378-73297397 None:intergenic 60.0%
TGGGATTCGCAGGAAAGCCG+CGG - chr2.2:73297502-73297521 MS.gene047186:CDS 60.0%
GATTCGCAGGAAAGCCGCGG+CGG - chr2.2:73297505-73297524 MS.gene047186:CDS 65.0%
TGGCAAACCCGTGGCAGGCA+TGG + chr2.2:73297373-73297392 None:intergenic 65.0%
!! GCTGCTGTTGGTGCTGGTGC+TGG + chr2.2:73296860-73296879 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 73296846 73297799 73296846 ID=MS.gene047186
chr2.2 mRNA 73296846 73297799 73296846 ID=MS.gene047186.t1;Parent=MS.gene047186
chr2.2 exon 73296846 73297799 73296846 ID=MS.gene047186.t1.exon1;Parent=MS.gene047186.t1
chr2.2 CDS 73296846 73297799 73296846 ID=cds.MS.gene047186.t1;Parent=MS.gene047186.t1
Gene Sequence

>MS.gene047186

ATGTCTCTCAACCAGCACCAGCACCAACAGCAGCAACCATCGCATGTCCACCCTCAAATCTGGCAGACATTAACCGGCACTGCTGTCCAAATACCAAAGTTGCATTCAAGAGTTTACTATTTTCCACAAGGCCACTTAGAATATGCTTCTCCTCCTTCCTCCTCCAATGCTTACATTCACTCCCTTCATGATCTTCAACGTTTTCGTCCTTTCACTATCTGTATCATCTCCGCGGTTGATCTCCTCGCCGATCCTCACACTGATGAGGTCTTCGCTAAACTGCTACTCACTCCAATAACCAATGATGTTGATGCCTGTGTCCAAGACCCTCATGAGGTTCCCAATTGCAACGACGATGATGAGGTAATTGGTTCGTTTACAAGGACTCTCGCCCCTACTAATGTAAGCAAGCATACATTTTACATACCTCGGTTTTGTGCTGAAAATATCTTCCCTCCGCTTGGCATGGAGATTTCTCAAAACCTCCTCGTCACTGACGTTCATGGTGAAGTCTGGAAGTTTCACCATGCCTGCCACGGGTTTGCCAAGAGAAACGTGTTTTATATCCCTGAATGGAGTTCGTTTGTTAAGAGGAAGAAACTCGACGTCGGAGATGCTGTTGTTTTCATGAAAAACTCAACGGGAAAATTATTTGTTGGGATTCGCAGGAAAGCCGCGGCGGAGCAAAAGAAAGATGAGTTGGAGAAGGCAGTAATGGAGGCAGTGAAGTTGGCAGAGGAGAACAAGCCATTTGAAATTGTTTACTATCCTCGAGGTGATGATTGGCATGATTTTGTAGTGGATGCTAATATTGTTGATGAATCAATGAAAATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTGATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTACTGTATCTCGTACGTCTAATTTATGGCGCATGCTACAGGTATAA
Protein sequence

>MS.gene047186.t1

MSLNQHQHQQQQPSHVHPQIWQTLTGTAVQIPKLHSRVYYFPQGHLEYASPPSSSNAYIHSLHDLQRFRPFTICIISAVDLLADPHTDEVFAKLLLTPITNDVDACVQDPHEVPNCNDDDEVIGSFTRTLAPTNVSKHTFYIPRFCAENIFPPLGMEISQNLLVTDVHGEVWKFHHACHGFAKRNVFYIPEWSSFVKRKKLDVGDAVVFMKNSTGKLFVGIRRKAAAEQKKDELEKAVMEAVKLAEENKPFEIVYYPRGDDWHDFVVDANIVDESMKIQWNPRMRVKMKTDKSSRIPYQGTISTVSRTSNLWRMLQV