AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047292

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047292.t1 MTR_2g026250 90.682 601 49 3 1 597 1 598 0.0 1122
MS.gene047292.t1 MTR_4g133660 54.711 605 204 9 1 596 1 544 0.0 613
MS.gene047292.t1 MTR_5g097480 47.273 605 248 12 1 595 1 544 0.0 523
MS.gene047292.t1 MTR_3g056110 54.588 425 189 3 174 595 119 542 5.54e-170 494
MS.gene047292.t1 MTR_5g094450 56.959 388 162 3 212 595 146 532 6.82e-160 468
MS.gene047292.t1 MTR_2g082090 54.712 382 166 2 216 594 201 578 5.06e-148 439
MS.gene047292.t1 MTR_1g029420 46.900 371 196 1 225 595 223 592 3.31e-126 384
MS.gene047292.t1 MTR_3g089055 46.900 371 196 1 225 595 201 570 1.28e-122 374
MS.gene047292.t1 MTR_3g089055 46.900 371 196 1 225 595 196 565 1.44e-122 374
MS.gene047292.t1 MTR_7g027190 34.596 396 222 6 222 596 295 674 3.43e-71 242
MS.gene047292.t1 MTR_4g064160 32.275 378 246 3 222 594 311 683 8.31e-64 222
MS.gene047292.t1 MTR_4g064200 32.804 378 242 6 222 594 279 649 1.14e-62 219
MS.gene047292.t1 MTR_4g064150 30.688 378 256 4 222 594 356 732 1.10e-61 218
MS.gene047292.t1 MTR_7g062120 30.400 375 259 2 222 595 367 740 1.28e-60 214
MS.gene047292.t1 MTR_7g057230 33.777 376 232 6 225 594 292 656 3.87e-59 209
MS.gene047292.t1 MTR_2g097310 32.095 377 246 6 222 594 267 637 5.60e-59 208
MS.gene047292.t1 MTR_2g097390 30.851 376 252 4 222 594 270 640 6.31e-59 208
MS.gene047292.t1 MTR_6g047750 33.333 393 217 9 225 595 293 662 1.10e-58 208
MS.gene047292.t1 MTR_2g097463 29.947 374 257 3 222 594 285 654 4.59e-56 201
MS.gene047292.t1 MTR_2g097467 29.708 377 260 4 219 594 281 653 8.09e-56 200
MS.gene047292.t1 MTR_2g097350 31.481 378 247 6 222 594 269 639 9.02e-56 199
MS.gene047292.t1 MTR_2g097473 30.933 375 252 6 222 594 284 653 1.43e-55 199
MS.gene047292.t1 MTR_2g097410 29.894 378 257 5 222 594 366 740 1.93e-55 200
MS.gene047292.t1 MTR_4g064120 29.211 380 255 5 222 592 249 623 2.19e-55 198
MS.gene047292.t1 MTR_4g064180 28.947 380 256 5 222 592 249 623 7.64e-54 194
MS.gene047292.t1 MTR_3g065980 31.218 394 229 11 224 596 169 541 2.86e-53 191
MS.gene047292.t1 MTR_4g104020 31.701 388 242 6 222 596 144 521 6.49e-53 189
MS.gene047292.t1 MTR_7g074650 32.425 367 232 7 230 595 441 792 2.20e-51 189
MS.gene047292.t1 MTR_8g442410 30.287 383 251 6 222 595 150 525 5.83e-49 179
MS.gene047292.t1 MTR_4g097080 29.944 354 235 6 250 595 155 503 1.07e-48 177
MS.gene047292.t1 MTR_3g022830 27.231 437 236 10 218 595 23 436 5.47e-48 174
MS.gene047292.t1 MTR_2g097380 28.818 347 215 4 249 594 245 560 5.56e-45 168
MS.gene047292.t1 MTR_4g102790 31.461 356 223 9 251 594 315 661 4.06e-44 167
MS.gene047292.t1 MTR_5g015490 29.282 362 233 8 250 595 136 490 7.07e-44 164
MS.gene047292.t1 MTR_4g077760 31.161 353 222 10 251 595 216 555 4.85e-40 154
MS.gene047292.t1 MTR_1g096030 30.688 378 208 14 251 595 103 459 2.34e-39 150
MS.gene047292.t1 MTR_4g076020 29.363 361 218 11 244 595 97 429 4.90e-37 143
MS.gene047292.t1 MTR_2g034250 26.357 387 266 7 222 595 205 585 1.38e-36 144
MS.gene047292.t1 MTR_3g072710 27.778 360 225 10 259 596 155 501 5.36e-36 142
MS.gene047292.t1 MTR_2g034260 25.840 387 267 9 222 595 205 584 1.48e-34 138
MS.gene047292.t1 MTR_2g034280 25.714 385 268 9 222 595 198 575 1.50e-34 138
MS.gene047292.t1 MTR_1g069725 28.994 338 218 9 263 596 145 464 5.88e-34 135
MS.gene047292.t1 MTR_5g058860 27.654 358 233 12 256 596 150 498 8.39e-33 132
MS.gene047292.t1 MTR_7g109580 27.089 347 237 8 256 595 217 554 1.40e-32 132
MS.gene047292.t1 MTR_2g099110 27.062 388 252 9 228 595 75 451 6.55e-32 129
MS.gene047292.t1 MTR_2g089100 26.055 403 257 11 224 594 49 442 1.24e-31 128
MS.gene047292.t1 MTR_4g026485 25.858 379 252 11 226 596 268 625 2.27e-28 120
MS.gene047292.t1 MTR_1g086970 25.143 350 239 8 259 595 139 478 7.46e-28 117
MS.gene047292.t1 MTR_5g015950 26.168 321 201 6 308 594 127 445 1.52e-27 116
MS.gene047292.t1 MTR_3g053270 26.168 321 201 6 308 594 127 445 1.52e-27 116
MS.gene047292.t1 MTR_4g095500 24.940 417 262 9 224 594 56 467 2.64e-27 115
MS.gene047292.t1 MTR_5g009080 31.050 219 138 5 228 441 57 267 1.67e-25 110
MS.gene047292.t1 MTR_5g009080 35.915 142 90 1 456 596 338 479 1.82e-22 101
MS.gene047292.t1 MTR_8g093070 27.042 355 217 16 255 594 168 495 3.84e-24 106
MS.gene047292.t1 MTR_4g076140 39.007 141 85 1 456 595 329 469 5.55e-24 105
MS.gene047292.t1 MTR_4g076140 29.952 207 137 3 228 433 49 248 5.57e-23 102
MS.gene047292.t1 MTR_0092s0100 25.330 379 255 11 226 596 374 732 9.26e-24 106
MS.gene047292.t1 MTR_0092s0100 25.330 379 255 11 226 596 371 729 9.63e-24 106
MS.gene047292.t1 MTR_8g020840 24.688 401 250 15 226 595 169 548 3.49e-22 100
MS.gene047292.t1 MTR_3g022580 30.000 110 77 0 486 595 74 183 5.81e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047292.t1 AT4G17230 47.088 601 236 10 1 595 1 525 2.82e-178 515
MS.gene047292.t1 AT4G17230 47.088 601 236 10 1 595 1 525 2.82e-178 515
MS.gene047292.t1 AT5G48150 55.581 430 186 4 168 595 64 490 4.41e-169 490
MS.gene047292.t1 AT5G48150 55.581 430 186 4 168 595 64 490 4.41e-169 490
MS.gene047292.t1 AT5G48150 55.581 430 186 4 168 595 64 490 4.41e-169 490
MS.gene047292.t1 AT5G48150 55.581 430 186 4 168 595 64 490 4.41e-169 490
MS.gene047292.t1 AT1G50600 52.349 447 202 5 151 595 89 526 2.96e-160 469
MS.gene047292.t1 AT2G04890 57.662 385 155 2 211 595 37 413 5.74e-160 464
MS.gene047292.t1 AT1G50600 52.349 447 202 5 151 595 160 597 1.11e-159 470
MS.gene047292.t1 AT1G50600 54.028 422 189 3 175 595 184 601 1.75e-159 469
MS.gene047292.t1 AT1G21450 45.526 380 205 2 217 595 215 593 1.37e-120 369
MS.gene047292.t1 AT1G07530 31.746 378 247 4 223 594 392 764 1.22e-61 218
MS.gene047292.t1 AT3G03450 33.245 376 237 9 224 595 180 545 1.43e-60 211
MS.gene047292.t1 AT5G52510 33.908 348 205 7 263 595 303 640 1.23e-59 210
MS.gene047292.t1 AT5G59450 30.423 378 258 4 222 595 222 598 4.46e-59 208
MS.gene047292.t1 AT2G37650 31.135 379 249 4 222 595 330 701 9.30e-59 209
MS.gene047292.t1 AT2G37650 31.135 379 249 4 222 595 342 713 9.47e-59 209
MS.gene047292.t1 AT5G17490 30.303 495 303 16 125 595 40 516 2.22e-58 204
MS.gene047292.t1 AT3G46600 30.563 373 256 3 224 595 1 371 8.30e-58 199
MS.gene047292.t1 AT3G46600 30.667 375 257 3 222 595 175 547 1.12e-57 203
MS.gene047292.t1 AT3G46600 30.667 375 257 3 222 595 207 579 1.74e-57 203
MS.gene047292.t1 AT2G29060 30.789 380 250 6 223 594 280 654 8.21e-57 202
MS.gene047292.t1 AT1G14920 31.593 383 229 10 224 595 169 529 9.17e-57 200
MS.gene047292.t1 AT2G29060 30.789 380 250 6 223 594 317 691 1.34e-56 202
MS.gene047292.t1 AT2G29065 30.628 382 255 7 222 594 247 627 3.06e-54 195
MS.gene047292.t1 AT2G01570 31.414 382 231 11 224 595 221 581 4.61e-54 194
MS.gene047292.t1 AT1G50420 28.837 430 238 8 228 596 58 480 4.77e-54 191
MS.gene047292.t1 AT1G66350 31.579 380 229 12 224 596 152 507 4.89e-54 192
MS.gene047292.t1 AT1G07520 30.366 382 255 8 222 594 149 528 2.77e-51 185
MS.gene047292.t1 AT1G07520 30.366 382 255 8 222 594 149 528 2.77e-51 185
MS.gene047292.t1 AT1G07520 29.659 381 259 6 222 594 313 692 1.31e-50 186
MS.gene047292.t1 AT1G07520 29.659 381 259 6 222 594 314 693 1.49e-50 186
MS.gene047292.t1 AT3G54220 31.165 369 235 8 230 594 296 649 1.78e-46 174
MS.gene047292.t1 AT5G66770 33.811 349 213 7 255 595 246 584 7.89e-45 168
MS.gene047292.t1 AT3G50650 32.286 350 219 7 255 595 202 542 1.62e-43 164
MS.gene047292.t1 AT4G37650 29.820 389 246 10 228 595 147 529 9.79e-42 158
MS.gene047292.t1 AT1G63100 28.535 389 249 7 227 594 273 653 1.56e-41 160
MS.gene047292.t1 AT1G63100 28.535 389 249 7 227 594 248 628 1.74e-41 159
MS.gene047292.t1 AT5G41920 27.926 376 244 10 228 595 44 400 1.34e-40 152
MS.gene047292.t1 AT1G55580 25.817 306 203 6 305 595 149 445 6.36e-30 123
MS.gene047292.t1 AT4G08250 25.926 351 247 7 251 594 132 476 2.74e-29 122
MS.gene047292.t1 AT3G49950 23.967 363 251 10 250 594 52 407 6.17e-24 105
MS.gene047292.t1 AT3G60630 24.716 352 230 10 261 596 291 623 1.07e-22 102
MS.gene047292.t1 AT2G45160 25.978 358 215 13 260 596 312 640 2.41e-21 99.0
MS.gene047292.t1 AT4G00150 23.873 377 257 11 224 596 205 555 8.29e-20 93.6
MS.gene047292.t1 AT3G13840 23.464 358 245 10 250 595 170 510 6.89e-19 90.5
MS.gene047292.t1 AT4G36710 23.969 388 259 12 222 595 120 485 3.94e-18 88.2
MS.gene047292.t1 AT5G67411 29.101 189 121 5 353 532 14 198 2.01e-12 67.0

Find 109 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAAGCAAACTGAAAATA+TGG 0.186262 2.1:-63907538 None:intergenic
AAGATTTCAATAAAGATTAT+AGG 0.216624 2.1:+63908301 MS.gene047292:CDS
TCAAAGAACCAATGTTTCTT+TGG 0.269262 2.1:+63906745 MS.gene047292:CDS
TGAGAAAATTGTGGTGAAAA+AGG 0.281191 2.1:-63906884 None:intergenic
AAAGCTCAATTTCTTTCATT+TGG 0.293718 2.1:-63906991 None:intergenic
CCTCCGTTCGTTCGTGTAAC+AGG 0.298231 2.1:+63907688 MS.gene047292:CDS
ACTGTGATCCTTGTGCAATT+TGG 0.334053 2.1:-63907624 None:intergenic
GATTTCAGAGCTAGGCTATA+AGG 0.338912 2.1:+63907123 MS.gene047292:CDS
TTGCAAATCAATTCAAATAA+TGG 0.345919 2.1:+63906713 MS.gene047292:CDS
GCTGTCGATGATATCTGAAT+TGG 0.360875 2.1:-63907170 None:intergenic
TTAACACTATTGAACTCAAA+TGG 0.361949 2.1:-63907802 None:intergenic
TTTCTTTGGAAGCAAGCAAC+TGG 0.364496 2.1:+63906759 MS.gene047292:CDS
TAAGCACCTAACCTTTGAAT+TGG 0.365266 2.1:-63907391 None:intergenic
TCTTCTTTCAAGTCTAGCTT+TGG 0.368681 2.1:-63907268 None:intergenic
GTTGAAAGACATGAACTGTT+TGG 0.373187 2.1:+63908192 MS.gene047292:CDS
AATTTGAGACCAATCATATT+CGG 0.388708 2.1:-63907233 None:intergenic
TGTGCACAGTTTGTATTTGA+TGG 0.403494 2.1:+63907301 MS.gene047292:CDS
GAAAGAAATTGAGCTTTCAT+TGG 0.409492 2.1:+63906997 MS.gene047292:CDS
TTCAATAGTGTTAAAATGTA+TGG 0.409922 2.1:+63907811 MS.gene047292:CDS
TCTGTCTCAAAGACTGTTGC+TGG 0.413091 2.1:-63906661 None:intergenic
GAATCGGAGATTTCATTGTT+AGG 0.417405 2.1:+63907145 MS.gene047292:CDS
AGAGCTAGGGTGGAATCATC+AGG 0.423843 2.1:+63907430 MS.gene047292:CDS
GTATGGATGTGAGGTTCAAC+TGG 0.437985 2.1:+63907828 MS.gene047292:CDS
ATCAAAAGCTATGAATTCAT+TGG 0.443080 2.1:-63906819 None:intergenic
AGCTGCCAGTCCAACTTAAA+CGG 0.448434 2.1:+63907190 MS.gene047292:CDS
GCCTAAGGTCGTGTTATTTG+TGG 0.450027 2.1:+63907981 MS.gene047292:CDS
GGTTCTCCAATTCAAAGGTT+AGG 0.459338 2.1:+63907385 MS.gene047292:CDS
GAAATTGAGCTTTCATTGGT+AGG 0.460607 2.1:+63907001 MS.gene047292:CDS
GGTGGAAGACTTGATATTGT+AGG 0.469286 2.1:+63907742 MS.gene047292:CDS
GGTACCGATATAGAAGTATT+CGG 0.469425 2.1:+63907022 MS.gene047292:CDS
TGTAAGCAAACTGAAAATAT+GGG 0.469553 2.1:-63907537 None:intergenic
TGTTGGAGGGATTAAGAGCT+AGG 0.473015 2.1:+63907416 MS.gene047292:CDS
TGCTTCACAAATGACAGCAT+TGG 0.474342 2.1:-63907566 None:intergenic
GAATAACGTGTTGGGAAAGA+TGG 0.476145 2.1:+63907351 MS.gene047292:CDS
GCTGCCGAATACTTCTATAT+CGG 0.477399 2.1:-63907026 None:intergenic
TCCATGCTCACACTCTCGTC+CGG 0.488477 2.1:-63907916 None:intergenic
AAGGTACATCCGAATATGAT+TGG 0.489420 2.1:+63907224 MS.gene047292:CDS
GTGAAGGCTACCATTTGAGT+TGG 0.490964 2.1:-63907056 None:intergenic
ACCTAACGGCGATACATTTG+TGG 0.492427 2.1:-63906927 None:intergenic
TTATGTGCACATCTTCTGCT+TGG 0.493993 2.1:+63908370 MS.gene047292:CDS
CTGTCGATGATATCTGAATT+GGG 0.497389 2.1:-63907169 None:intergenic
AGCTGTCTGTCCAACTCAAA+TGG 0.502650 2.1:+63907046 MS.gene047292:CDS
GGAAAGATGGTATCGGTTGG+CGG 0.502999 2.1:+63907364 MS.gene047292:CDS
GGATTCATGAATAACGTGTT+GGG 0.509988 2.1:+63907343 MS.gene047292:CDS
AGGATTCATGAATAACGTGT+TGG 0.512384 2.1:+63907342 MS.gene047292:CDS
AGAGCTAGGCTATAAGGAAT+CGG 0.514017 2.1:+63907129 MS.gene047292:CDS
GGCTACCGTTTAAGTTGGAC+TGG 0.514240 2.1:-63907195 None:intergenic
GTGGAGGCTACCGTTTAAGT+TGG 0.523399 2.1:-63907200 None:intergenic
ATTCTCTTCTTATCATCTCT+AGG 0.523465 2.1:-63908102 None:intergenic
GATTCTCCTTCATCTTCTAG+TGG 0.524436 2.1:+63906839 MS.gene047292:CDS
CATTATCTTGAGATGTACCT+TGG 0.526204 2.1:-63907078 None:intergenic
TCAGTCACCGAGGGACTCAA+TGG 0.526464 2.1:-63908258 None:intergenic
GACATGAACTGTTTGGAAAG+TGG 0.527553 2.1:+63908199 MS.gene047292:CDS
CTCATCAACTCATTGCTCGT+TGG 0.531720 2.1:-63907487 None:intergenic
CAAAAGAAACGTAGTTCAGC+CGG 0.533280 2.1:+63906623 MS.gene047292:CDS
GTTAGGTGCTTACATGTTGG+AGG 0.539755 2.1:+63907402 MS.gene047292:CDS
CTTAAAGTCTCAGCAAACCT+TGG 0.540575 2.1:-63908039 None:intergenic
GGTATGTGATGCAAAGCAAA+AGG 0.541821 2.1:-63907895 None:intergenic
GACTGTTGCTGGTAGTATAA+CGG 0.543849 2.1:-63906650 None:intergenic
GCTTGCTTCCAAAGAAACAT+TGG 0.545908 2.1:-63906753 None:intergenic
CATGCTCTCAAGCATAAACC+TGG 0.546708 2.1:+63907661 MS.gene047292:CDS
ACCGTTACCATTGAGTCCCT+CGG 0.546824 2.1:+63908251 MS.gene047292:CDS
CGAACGAACGGAGGTCCGCC+AGG 0.546839 2.1:-63907679 None:intergenic
AAGGTTAGGTGCTTACATGT+TGG 0.546985 2.1:+63907399 MS.gene047292:CDS
TTGGCGGTTCTCCAATTCAA+AGG 0.547368 2.1:+63907380 MS.gene047292:CDS
GCATGGAGAATCATAGAGAC+AGG 0.548705 2.1:+63907932 MS.gene047292:CDS
GGAGGGATTAAGAGCTAGGG+TGG 0.553683 2.1:+63907420 MS.gene047292:CDS
CTGGTAGTATAACGGTATAC+CGG 0.553841 2.1:-63906642 None:intergenic
TCCACAAATAACACGACCTT+AGG 0.558882 2.1:-63907982 None:intergenic
CTTGAGATGTACCTTGGTGA+AGG 0.559050 2.1:-63907072 None:intergenic
ACCGGACGAGAGTGTGAGCA+TGG 0.561598 2.1:+63907915 MS.gene047292:CDS
TACATTTGTGGTGTTGTCAG+AGG 0.565214 2.1:-63906915 None:intergenic
ACCACAAATGTATCGCCGTT+AGG 0.567915 2.1:+63906926 MS.gene047292:CDS
TTCTTTGGAAGCAAGCAACT+GGG 0.569062 2.1:+63906760 MS.gene047292:CDS
GAGTCATAGTGAGAAAATTG+TGG 0.570992 2.1:-63906893 None:intergenic
CATATTCGGATGTACCTTGG+TGG 0.571085 2.1:-63907219 None:intergenic
CAATCGTTTCATGCTAGAGG+TGG 0.577074 2.1:+63907724 MS.gene047292:CDS
AATCATATTCGGATGTACCT+TGG 0.577241 2.1:-63907222 None:intergenic
TCACAATCGTTTCATGCTAG+AGG 0.578887 2.1:+63907721 MS.gene047292:CDS
GAATTAATGATTTCAGAGCT+AGG 0.580583 2.1:+63907115 MS.gene047292:CDS
TTAGGTGCTTACATGTTGGA+GGG 0.580819 2.1:+63907403 MS.gene047292:CDS
GCATTACCACTAGAAGATGA+AGG 0.589681 2.1:-63906845 None:intergenic
GTGGTAATGCATTCAACACC+AGG 0.592949 2.1:+63906858 MS.gene047292:CDS
CGTTAACATCATAGCTTGTG+AGG 0.593625 2.1:+63908158 MS.gene047292:CDS
ATTCGGATGTACCTTGGTGG+AGG 0.599110 2.1:-63907216 None:intergenic
CGTGTTGGGAAAGATGGTAT+CGG 0.599692 2.1:+63907357 MS.gene047292:CDS
TTGGGAAAGATGGTATCGGT+TGG 0.601702 2.1:+63907361 MS.gene047292:CDS
GGCTACTTGAGAAGATTCCA+AGG 0.613501 2.1:-63906798 None:intergenic
GTTAACATCATAGCTTGTGA+GGG 0.618537 2.1:+63908159 MS.gene047292:CDS
GTTGGAGGGATTAAGAGCTA+GGG 0.618696 2.1:+63907417 MS.gene047292:CDS
ACCGAGGGACTCAATGGTAA+CGG 0.634336 2.1:-63908252 None:intergenic
GCACTAGAGTAAACACCTAA+CGG 0.650576 2.1:-63906941 None:intergenic
ATTGACTTCCAAATTGCACA+AGG 0.655189 2.1:+63907616 MS.gene047292:CDS
GTTAGGTGTTTACTCTAGTG+CGG 0.655711 2.1:+63906943 MS.gene047292:CDS
ATGAACTGTTTGGAAAGTGG+AGG 0.656082 2.1:+63908202 MS.gene047292:CDS
TGTTAAAATGTATGGATGTG+AGG 0.656156 2.1:+63907819 MS.gene047292:CDS
GAGCTAGGGTGGAATCATCA+GGG 0.659667 2.1:+63907431 MS.gene047292:CDS
ATGTTGCTATCAATTTAGCG+TGG 0.662028 2.1:+63908337 MS.gene047292:CDS
AATGTTTGAATCAATTGATG+TGG 0.662206 2.1:+63908074 MS.gene047292:CDS
ATACCTGTTACACGAACGAA+CGG 0.677244 2.1:-63907691 None:intergenic
CTAACTGAATCAGTCACCGA+GGG 0.680621 2.1:-63908267 None:intergenic
CCTGTTACACGAACGAACGG+AGG 0.680914 2.1:-63907688 None:intergenic
GTTTACTCTAGTGCGGACGA+TGG 0.696726 2.1:+63906950 MS.gene047292:CDS
TAATGCAGAACAACACTGTG+TGG 0.712966 2.1:+63908125 MS.gene047292:CDS
GCTCTCAAGCATAAACCTGG+CGG 0.733186 2.1:+63907664 MS.gene047292:CDS
TCAAATGGTAGCCTTCACCA+AGG 0.752690 2.1:+63907061 MS.gene047292:CDS
TCTAACTGAATCAGTCACCG+AGG 0.753321 2.1:-63908268 None:intergenic
TTAAACGGTAGCCTCCACCA+AGG 0.778392 2.1:+63907205 MS.gene047292:CDS
AAATTGCACAAGGATCACAG+TGG 0.820097 2.1:+63907626 MS.gene047292:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTTCAATAAAGATTAT+AGG + chr2.1:63908301-63908320 MS.gene047292:CDS 15.0%
!! TTCAATAGTGTTAAAATGTA+TGG + chr2.1:63907811-63907830 MS.gene047292:CDS 20.0%
!! TTGCAAATCAATTCAAATAA+TGG + chr2.1:63906713-63906732 MS.gene047292:CDS 20.0%
! AAAGCTCAATTTCTTTCATT+TGG - chr2.1:63906994-63907013 None:intergenic 25.0%
! AATGTTTGAATCAATTGATG+TGG + chr2.1:63908074-63908093 MS.gene047292:CDS 25.0%
! ATCAAAAGCTATGAATTCAT+TGG - chr2.1:63906822-63906841 None:intergenic 25.0%
! ATGTAAGCAAACTGAAAATA+TGG - chr2.1:63907541-63907560 None:intergenic 25.0%
! TGTAAGCAAACTGAAAATAT+GGG - chr2.1:63907540-63907559 None:intergenic 25.0%
! TTAACACTATTGAACTCAAA+TGG - chr2.1:63907805-63907824 None:intergenic 25.0%
!! AATTTGAGACCAATCATATT+CGG - chr2.1:63907236-63907255 None:intergenic 25.0%
ATTCTCTTCTTATCATCTCT+AGG - chr2.1:63908105-63908124 None:intergenic 30.0%
GAAAGAAATTGAGCTTTCAT+TGG + chr2.1:63906997-63907016 MS.gene047292:CDS 30.0%
GAATTAATGATTTCAGAGCT+AGG + chr2.1:63907115-63907134 MS.gene047292:CDS 30.0%
TCAAAGAACCAATGTTTCTT+TGG + chr2.1:63906745-63906764 MS.gene047292:CDS 30.0%
TGAGAAAATTGTGGTGAAAA+AGG - chr2.1:63906887-63906906 None:intergenic 30.0%
TGTTAAAATGTATGGATGTG+AGG + chr2.1:63907819-63907838 MS.gene047292:CDS 30.0%
! GGTGATTTTCAAAAAGCAAT+AGG + chr2.1:63907322-63907341 MS.gene047292:CDS 30.0%
AAGGTACATCCGAATATGAT+TGG + chr2.1:63907224-63907243 MS.gene047292:CDS 35.0%
AATCATATTCGGATGTACCT+TGG - chr2.1:63907225-63907244 None:intergenic 35.0%
AGGATTCATGAATAACGTGT+TGG + chr2.1:63907342-63907361 MS.gene047292:CDS 35.0%
ATGTTGCTATCAATTTAGCG+TGG + chr2.1:63908337-63908356 MS.gene047292:CDS 35.0%
CATTATCTTGAGATGTACCT+TGG - chr2.1:63907081-63907100 None:intergenic 35.0%
CTGTCGATGATATCTGAATT+GGG - chr2.1:63907172-63907191 None:intergenic 35.0%
GAAATTGAGCTTTCATTGGT+AGG + chr2.1:63907001-63907020 MS.gene047292:CDS 35.0%
GAATCGGAGATTTCATTGTT+AGG + chr2.1:63907145-63907164 MS.gene047292:CDS 35.0%
GAGTCATAGTGAGAAAATTG+TGG - chr2.1:63906896-63906915 None:intergenic 35.0%
GGATTCATGAATAACGTGTT+GGG + chr2.1:63907343-63907362 MS.gene047292:CDS 35.0%
GTTGAAAGACATGAACTGTT+TGG + chr2.1:63908192-63908211 MS.gene047292:CDS 35.0%
TAAGCACCTAACCTTTGAAT+TGG - chr2.1:63907394-63907413 None:intergenic 35.0%
TCAGCAAACCTTGGTAAAAA+TGG - chr2.1:63908033-63908052 None:intergenic 35.0%
TGTGCACAGTTTGTATTTGA+TGG + chr2.1:63907301-63907320 MS.gene047292:CDS 35.0%
! AGGAAAAGATTTTCAATGGC+GGG + chr2.1:63908222-63908241 MS.gene047292:CDS 35.0%
! ATTGACTTCCAAATTGCACA+AGG + chr2.1:63907616-63907635 MS.gene047292:CDS 35.0%
! GAGGAAAAGATTTTCAATGG+CGG + chr2.1:63908221-63908240 MS.gene047292:CDS 35.0%
! GTGGAGGAAAAGATTTTCAA+TGG + chr2.1:63908218-63908237 MS.gene047292:CDS 35.0%
! GTTAACATCATAGCTTGTGA+GGG + chr2.1:63908159-63908178 MS.gene047292:CDS 35.0%
! TCTTCTTTCAAGTCTAGCTT+TGG - chr2.1:63907271-63907290 None:intergenic 35.0%
! TTTTGCTTTGCATCACATAC+CGG + chr2.1:63907897-63907916 MS.gene047292:CDS 35.0%
!! ACACTTCTCCATTTTTACCA+AGG + chr2.1:63908022-63908041 MS.gene047292:CDS 35.0%
!! GGTACCGATATAGAAGTATT+CGG + chr2.1:63907022-63907041 MS.gene047292:CDS 35.0%
AAATTGCACAAGGATCACAG+TGG + chr2.1:63907626-63907645 MS.gene047292:CDS 40.0%
AAGGTTAGGTGCTTACATGT+TGG + chr2.1:63907399-63907418 MS.gene047292:CDS 40.0%
ACTGTGATCCTTGTGCAATT+TGG - chr2.1:63907627-63907646 None:intergenic 40.0%
AGAGCTAGGCTATAAGGAAT+CGG + chr2.1:63907129-63907148 MS.gene047292:CDS 40.0%
ATACCTGTTACACGAACGAA+CGG - chr2.1:63907694-63907713 None:intergenic 40.0%
ATGAACTGTTTGGAAAGTGG+AGG + chr2.1:63908202-63908221 MS.gene047292:CDS 40.0%
CAAAAGAAACGTAGTTCAGC+CGG + chr2.1:63906623-63906642 MS.gene047292:CDS 40.0%
CTGGTAGTATAACGGTATAC+CGG - chr2.1:63906645-63906664 None:intergenic 40.0%
CTTAAAGTCTCAGCAAACCT+TGG - chr2.1:63908042-63908061 None:intergenic 40.0%
GAATAACGTGTTGGGAAAGA+TGG + chr2.1:63907351-63907370 MS.gene047292:CDS 40.0%
GACATGAACTGTTTGGAAAG+TGG + chr2.1:63908199-63908218 MS.gene047292:CDS 40.0%
GACTGTTGCTGGTAGTATAA+CGG - chr2.1:63906653-63906672 None:intergenic 40.0%
GATTCTCCTTCATCTTCTAG+TGG + chr2.1:63906839-63906858 MS.gene047292:CDS 40.0%
GATTTCAGAGCTAGGCTATA+AGG + chr2.1:63907123-63907142 MS.gene047292:CDS 40.0%
GCACTAGAGTAAACACCTAA+CGG - chr2.1:63906944-63906963 None:intergenic 40.0%
GCATTACCACTAGAAGATGA+AGG - chr2.1:63906848-63906867 None:intergenic 40.0%
GCTGCCGAATACTTCTATAT+CGG - chr2.1:63907029-63907048 None:intergenic 40.0%
GCTGTCGATGATATCTGAAT+TGG - chr2.1:63907173-63907192 None:intergenic 40.0%
GCTTGCTTCCAAAGAAACAT+TGG - chr2.1:63906756-63906775 None:intergenic 40.0%
GGTATGTGATGCAAAGCAAA+AGG - chr2.1:63907898-63907917 None:intergenic 40.0%
GGTGGAAGACTTGATATTGT+AGG + chr2.1:63907742-63907761 MS.gene047292:CDS 40.0%
GGTTCTCCAATTCAAAGGTT+AGG + chr2.1:63907385-63907404 MS.gene047292:CDS 40.0%
GTTAGGTGTTTACTCTAGTG+CGG + chr2.1:63906943-63906962 MS.gene047292:CDS 40.0%
TAATGCAGAACAACACTGTG+TGG + chr2.1:63908125-63908144 MS.gene047292:CDS 40.0%
TCACAATCGTTTCATGCTAG+AGG + chr2.1:63907721-63907740 MS.gene047292:CDS 40.0%
TCCACAAATAACACGACCTT+AGG - chr2.1:63907985-63908004 None:intergenic 40.0%
TGCTTCACAAATGACAGCAT+TGG - chr2.1:63907569-63907588 None:intergenic 40.0%
TTAGGTGCTTACATGTTGGA+GGG + chr2.1:63907403-63907422 MS.gene047292:CDS 40.0%
TTATGTGCACATCTTCTGCT+TGG + chr2.1:63908370-63908389 MS.gene047292:CDS 40.0%
TTGTGGTGAAAAAGGATTCC+TGG - chr2.1:63906879-63906898 None:intergenic 40.0%
! CGTTAACATCATAGCTTGTG+AGG + chr2.1:63908158-63908177 MS.gene047292:CDS 40.0%
! GGAGAAGCAATTTTGTACCT+TGG + chr2.1:63906781-63906800 MS.gene047292:CDS 40.0%
! TTCTTTGGAAGCAAGCAACT+GGG + chr2.1:63906760-63906779 MS.gene047292:CDS 40.0%
! TTTCTTTGGAAGCAAGCAAC+TGG + chr2.1:63906759-63906778 MS.gene047292:CDS 40.0%
!! TACATTTGTGGTGTTGTCAG+AGG - chr2.1:63906918-63906937 None:intergenic 40.0%
ACCACAAATGTATCGCCGTT+AGG + chr2.1:63906926-63906945 MS.gene047292:CDS 45.0%
ACCTAACGGCGATACATTTG+TGG - chr2.1:63906930-63906949 None:intergenic 45.0%
AGCTGCCAGTCCAACTTAAA+CGG + chr2.1:63907190-63907209 MS.gene047292:CDS 45.0%
AGCTGTCTGTCCAACTCAAA+TGG + chr2.1:63907046-63907065 MS.gene047292:CDS 45.0%
CAATCGTTTCATGCTAGAGG+TGG + chr2.1:63907724-63907743 MS.gene047292:CDS 45.0%
CATATTCGGATGTACCTTGG+TGG - chr2.1:63907222-63907241 None:intergenic 45.0%
CATGCTCTCAAGCATAAACC+TGG + chr2.1:63907661-63907680 MS.gene047292:CDS 45.0%
CGTGTTGGGAAAGATGGTAT+CGG + chr2.1:63907357-63907376 MS.gene047292:CDS 45.0%
CTAACTGAATCAGTCACCGA+GGG - chr2.1:63908270-63908289 None:intergenic 45.0%
CTCATCAACTCATTGCTCGT+TGG - chr2.1:63907490-63907509 None:intergenic 45.0%
CTTGAGATGTACCTTGGTGA+AGG - chr2.1:63907075-63907094 None:intergenic 45.0%
GCATGGAGAATCATAGAGAC+AGG + chr2.1:63907932-63907951 MS.gene047292:CDS 45.0%
GGCTACTTGAGAAGATTCCA+AGG - chr2.1:63906801-63906820 None:intergenic 45.0%
GTATGGATGTGAGGTTCAAC+TGG + chr2.1:63907828-63907847 MS.gene047292:CDS 45.0%
GTGAAGGCTACCATTTGAGT+TGG - chr2.1:63907059-63907078 None:intergenic 45.0%
GTTAGGTGCTTACATGTTGG+AGG + chr2.1:63907402-63907421 MS.gene047292:CDS 45.0%
GTTGGAGGGATTAAGAGCTA+GGG + chr2.1:63907417-63907436 MS.gene047292:CDS 45.0%
TCAAATGGTAGCCTTCACCA+AGG + chr2.1:63907061-63907080 MS.gene047292:CDS 45.0%
TCTAACTGAATCAGTCACCG+AGG - chr2.1:63908271-63908290 None:intergenic 45.0%
TCTGTCTCAAAGACTGTTGC+TGG - chr2.1:63906664-63906683 None:intergenic 45.0%
TGTTGGAGGGATTAAGAGCT+AGG + chr2.1:63907416-63907435 MS.gene047292:CDS 45.0%
TTGGCGGTTCTCCAATTCAA+AGG + chr2.1:63907380-63907399 MS.gene047292:CDS 45.0%
! GGTGAAGATTTTGTCGCCTA+AGG + chr2.1:63907966-63907985 MS.gene047292:CDS 45.0%
! GTGGTAATGCATTCAACACC+AGG + chr2.1:63906858-63906877 MS.gene047292:CDS 45.0%
!! GCCTAAGGTCGTGTTATTTG+TGG + chr2.1:63907981-63908000 MS.gene047292:CDS 45.0%
!! TTGGGAAAGATGGTATCGGT+TGG + chr2.1:63907361-63907380 MS.gene047292:CDS 45.0%
!!! TAGAGACAGGCTTTTGAGAC+TGG + chr2.1:63907945-63907964 MS.gene047292:CDS 45.0%
ACCGAGGGACTCAATGGTAA+CGG - chr2.1:63908255-63908274 None:intergenic 50.0%
ACCGTTACCATTGAGTCCCT+CGG + chr2.1:63908251-63908270 MS.gene047292:CDS 50.0%
AGAGCTAGGGTGGAATCATC+AGG + chr2.1:63907430-63907449 MS.gene047292:CDS 50.0%
ATTCGGATGTACCTTGGTGG+AGG - chr2.1:63907219-63907238 None:intergenic 50.0%
GAGCTAGGGTGGAATCATCA+GGG + chr2.1:63907431-63907450 MS.gene047292:CDS 50.0%
GCTCTCAAGCATAAACCTGG+CGG + chr2.1:63907664-63907683 MS.gene047292:CDS 50.0%
GTGGAGGCTACCGTTTAAGT+TGG - chr2.1:63907203-63907222 None:intergenic 50.0%
GTTTACTCTAGTGCGGACGA+TGG + chr2.1:63906950-63906969 MS.gene047292:CDS 50.0%
TTAAACGGTAGCCTCCACCA+AGG + chr2.1:63907205-63907224 MS.gene047292:CDS 50.0%
!! GGAAAGATGGTATCGGTTGG+CGG + chr2.1:63907364-63907383 MS.gene047292:CDS 50.0%
!! GGCTACCGTTTAAGTTGGAC+TGG - chr2.1:63907198-63907217 None:intergenic 50.0%
CCTCCGTTCGTTCGTGTAAC+AGG + chr2.1:63907688-63907707 MS.gene047292:CDS 55.0%
CCTGTTACACGAACGAACGG+AGG - chr2.1:63907691-63907710 None:intergenic 55.0%
GGAGGGATTAAGAGCTAGGG+TGG + chr2.1:63907420-63907439 MS.gene047292:CDS 55.0%
TCAGTCACCGAGGGACTCAA+TGG - chr2.1:63908261-63908280 None:intergenic 55.0%
TCCATGCTCACACTCTCGTC+CGG - chr2.1:63907919-63907938 None:intergenic 55.0%
! ACCGGACGAGAGTGTGAGCA+TGG + chr2.1:63907915-63907934 MS.gene047292:CDS 60.0%
CGAACGAACGGAGGTCCGCC+AGG - chr2.1:63907682-63907701 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 63906611 63908404 63906611 ID=MS.gene047292
chr2.1 mRNA 63906611 63908404 63906611 ID=MS.gene047292.t1;Parent=MS.gene047292
chr2.1 exon 63906611 63908404 63906611 ID=MS.gene047292.t1.exon1;Parent=MS.gene047292.t1
chr2.1 CDS 63906611 63908404 63906611 ID=cds.MS.gene047292.t1;Parent=MS.gene047292.t1
Gene Sequence

>MS.gene047292

ATGTATACTTCTCAAAAGAAACGTAGTTCAGCCGGTATACCGTTATACTACCAGCAACAGTCTTTGAGACAGATTGATTCAAACAACAATCGTTTCAAAACGTTGCAAATCAATTCAAATAATGGTAGTATCAGTCAAAGAACCAATGTTTCTTTGGAAGCAAGCAACTGGGAGAAGCAATTTTGTACCTTGGAATCTTCTCAAGTAGCCAATGAATTCATAGCTTTTGATTCTCCTTCATCTTCTAGTGGTAATGCATTCAACACCAGGAATCCTTTTTCACCACAATTTTCTCACTATGACTCCTCTGACAACACCACAAATGTATCGCCGTTAGGTGTTTACTCTAGTGCGGACGATGGTAGCTTTGAATTGAAGCACCAAATGAAAGAAATTGAGCTTTCATTGGTAGGTACCGATATAGAAGTATTCGGCAGCTGTCTGTCCAACTCAAATGGTAGCCTTCACCAAGGTACATCTCAAGATAATGAGTTTCAAATTGATGAATTAATGATTTCAGAGCTAGGCTATAAGGAATCGGAGATTTCATTGTTAGGTCCCAATTCAGATATCATCGACAGCTGCCAGTCCAACTTAAACGGTAGCCTCCACCAAGGTACATCCGAATATGATTGGTCTCAAATTGTAGAAATGATTCCAAAGCTAGACTTGAAAGAAGAACTTATTAGATGTGCACAGTTTGTATTTGATGGTGATTTTCAAAAAGCAATAGGATTCATGAATAACGTGTTGGGAAAGATGGTATCGGTTGGCGGTTCTCCAATTCAAAGGTTAGGTGCTTACATGTTGGAGGGATTAAGAGCTAGGGTGGAATCATCAGGGAGTGCAATTTATAATGCGTTGAAATGTGAAGAACCAACGAGCAATGAGTTGATGAGTGCTATGCACACTTTGTATCAGATTTGCCCATATTTTCAGTTTGCTTACATATCATCCAATGCTGTCATTTGTGAAGCAATGCAAAATGAATCAAGAATTCATATAATTGACTTCCAAATTGCACAAGGATCACAGTGGATGTTACTTTTGCATGCTCTCAAGCATAAACCTGGCGGACCTCCGTTCGTTCGTGTAACAGGTATCGATGATTCACAATCGTTTCATGCTAGAGGTGGAAGACTTGATATTGTAGGAAAAAAGCTAGAAGATTGTGCAAAAACTTGTAGAGTTCCATTTGAGTTCAATAGTGTTAAAATGTATGGATGTGAGGTTCAACTGGAAGATTTTGAAGTTCAACATGATGAAGTTCTTGCTGTGAATTTTCCTTTTGCTTTGCATCACATACCGGACGAGAGTGTGAGCATGGAGAATCATAGAGACAGGCTTTTGAGACTGGTGAAGATTTTGTCGCCTAAGGTCGTGTTATTTGTGGAGCAAGAGTCAAACACAAACACTTCTCCATTTTTACCAAGGTTTGCTGAGACTTTAAGTTATTATACTGCAATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAGTTGAAAGACATGAACTGTTTGGAAAGTGGAGGAAAAGATTTTCAATGGCGGGTTTTGTACCGTTACCATTGAGTCCCTCGGTGACTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA
Protein sequence

>MS.gene047292.t1

MYTSQKKRSSAGIPLYYQQQSLRQIDSNNNRFKTLQINSNNGSISQRTNVSLEASNWEKQFCTLESSQVANEFIAFDSPSSSSGNAFNTRNPFSPQFSHYDSSDNTTNVSPLGVYSSADDGSFELKHQMKEIELSLVGTDIEVFGSCLSNSNGSLHQGTSQDNEFQIDELMISELGYKESEISLLGPNSDIIDSCQSNLNGSLHQGTSEYDWSQIVEMIPKLDLKEELIRCAQFVFDGDFQKAIGFMNNVLGKMVSVGGSPIQRLGAYMLEGLRARVESSGSAIYNALKCEEPTSNELMSAMHTLYQICPYFQFAYISSNAVICEAMQNESRIHIIDFQIAQGSQWMLLLHALKHKPGGPPFVRVTGIDDSQSFHARGGRLDIVGKKLEDCAKTCRVPFEFNSVKMYGCEVQLEDFEVQHDEVLAVNFPFALHHIPDESVSMENHRDRLLRLVKILSPKVVLFVEQESNTNTSPFLPRFAETLSYYTAMFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERVERHELFGKWRKRFSMAGFVPLPLSPSVTDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY