AlfalfaGEDB | MS.gene047314

M. sativa cultivar XinJiangDaYe / MS.gene047314

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047314.t1	XP_004486346.1	89.3	84	4	2	1	80	1	83	2.00E-30	141.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047314.t1	O23310	79.5	88	9	2	1	80	1	87	5.0e-30	131.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047314.t1	I3TAX1	89.3	84	4	2	1	80	1	83	1.4e-30	141.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene047314.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047314.t1	MTR_2g026710	86.207	87	7	2	1	83	1	86	1.20e-46	146
MS.gene047314.t1	MTR_5g095740	76.344	93	11	2	1	83	1	92	1.20e-43	139
MS.gene047314.t1	MTR_3g058980	72.165	97	12	2	1	83	1	96	2.93e-42	136
MS.gene047314.t1	MTR_8g091720	81.818	77	13	1	7	83	22	97	7.46e-40	130
MS.gene047314.t1	MTR_7g100650	81.944	72	12	1	12	83	25	95	3.94e-38	125
MS.gene047314.t1	MTR_7g061270	81.944	72	12	1	12	83	25	95	3.96e-38	125
MS.gene047314.t1	MTR_4g133938	73.034	89	17	3	1	83	1	88	2.42e-37	122
MS.gene047314.t1	MTR_1g072790	75.000	76	18	1	8	83	24	98	1.57e-36	121
MS.gene047314.t1	MTR_8g093920	77.778	72	15	1	12	83	39	109	5.54e-36	120
MS.gene047314.t1	MTR_0392s0020	71.233	73	21	0	2	74	7	79	2.81e-34	114
MS.gene047314.t1	MTR_1g088860	80.952	63	12	0	12	74	35	97	2.34e-33	114
MS.gene047314.t1	MTR_4g119500	72.222	72	19	1	4	74	12	83	2.31e-32	109
MS.gene047314.t1	MTR_4g133952	66.667	78	24	2	3	80	49	124	5.22e-31	108
MS.gene047314.t1	MTR_1g039040	63.889	72	25	1	9	80	2	72	4.45e-30	105
MS.gene047314.t1	MTR_1g083070	72.581	62	17	0	13	74	4	65	2.39e-28	99.0
MS.gene047314.t1	MTR_1g029070	57.895	57	24	0	18	74	57	113	2.39e-17	72.4
MS.gene047314.t1	MTR_5g095900	46.753	77	38	1	1	74	1	77	5.14e-16	68.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047314.t1	AT4G14540	76.923	91	12	2	1	83	1	90	9.46e-44	139
MS.gene047314.t1	AT5G47640	89.855	69	6	1	12	80	26	93	3.47e-39	128
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	1.76e-37	122
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	2.71e-37	122
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	3.28e-37	122
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	3.28e-37	122
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	3.28e-37	122
MS.gene047314.t1	AT2G38880	75.000	80	18	2	2	80	9	87	4.45e-37	120
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	1.05e-36	121
MS.gene047314.t1	AT2G38880	72.289	83	21	2	2	83	9	90	1.05e-36	121
MS.gene047314.t1	AT2G38880	75.000	80	18	2	2	80	9	87	2.17e-36	120
MS.gene047314.t1	AT2G38880	75.000	80	18	2	2	80	9	87	2.17e-36	120
MS.gene047314.t1	AT2G38880	75.000	80	18	2	2	80	9	87	2.17e-36	120
MS.gene047314.t1	AT2G13570	77.778	72	15	1	12	83	35	105	3.21e-36	121
MS.gene047314.t1	AT2G37060	76.389	72	16	1	12	83	29	99	4.12e-36	120
MS.gene047314.t1	AT2G37060	76.389	72	16	1	12	83	29	99	4.12e-36	120
MS.gene047314.t1	AT2G37060	76.389	72	16	1	12	83	29	99	4.12e-36	120
MS.gene047314.t1	AT3G53340	75.342	73	17	1	8	80	24	95	7.45e-36	118
MS.gene047314.t1	AT3G53340	75.342	73	17	1	8	80	24	95	1.36e-35	119
MS.gene047314.t1	AT3G53340	75.342	73	17	1	8	80	24	95	1.36e-35	119
MS.gene047314.t1	AT2G47810	80.882	68	13	0	7	74	45	112	1.75e-35	118
MS.gene047314.t1	AT3G53340	75.342	73	17	1	8	80	24	95	4.81e-35	116
MS.gene047314.t1	AT3G53340	75.342	73	17	1	8	80	24	95	4.81e-35	116
MS.gene047314.t1	AT5G47670	68.116	69	21	1	12	80	28	95	5.50e-30	105
MS.gene047314.t1	AT5G47670	68.116	69	21	1	12	80	57	124	9.03e-30	105
MS.gene047314.t1	AT5G47670	68.116	69	21	1	12	80	57	124	9.03e-30	105
MS.gene047314.t1	AT1G21970	64.286	70	24	1	11	80	57	125	5.00e-29	103
MS.gene047314.t1	AT1G09030	66.176	68	21	1	13	80	3	68	7.65e-29	100
MS.gene047314.t1	AT5G08190	36.508	63	40	0	10	72	8	70	4.51e-11	55.8
MS.gene047314.t1	AT5G08190	36.508	63	40	0	10	72	8	70	4.73e-11	55.8
MS.gene047314.t1	AT5G23090	34.921	63	41	0	10	72	8	70	8.33e-11	55.1
MS.gene047314.t1	AT5G23090	34.921	63	41	0	10	72	8	70	8.93e-11	55.1
MS.gene047314.t1	AT5G23090	34.921	63	41	0	10	72	8	70	8.93e-11	55.1
MS.gene047314.t1	AT5G23090	34.921	63	41	0	10	72	8	70	8.93e-11	55.1

Find 13 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGAGATCTTTGCGTTTGC+TGG	0.274169	2.1:+63597587	None:intergenic
ATCCTACTCACGTTTGCTAT+TGG	0.294063	2.1:+63597626	None:intergenic
CGTGAGTAGGATCATGAAAA+AGG	0.331544	2.1:-63597615	MS.gene047314:CDS
GAGTTCATCAGTTTCATAAC+AGG	0.366706	2.1:-63597530	MS.gene047314:CDS
CAGGTTGCAGAGAGCAAGAT+AGG	0.404952	2.1:-63597655	MS.gene047314:CDS
AAACATTATGGATGGTGGTT+TGG	0.443613	2.1:-63596943	MS.gene047314:CDS
GAGGGTACAGTGAAACATTA+TGG	0.444819	2.1:-63596955	MS.gene047314:CDS
GAATCAGACAATGAGTCAGC+AGG	0.557061	2.1:-63597674	MS.gene047314:CDS
ATAAGTGTCAGAAAGAGAAG+AGG	0.567471	2.1:-63597496	MS.gene047314:CDS
TTCCAATAGCAAACGTGAGT+AGG	0.595976	2.1:-63597628	MS.gene047314:CDS
CAGTGAAACATTATGGATGG+TGG	0.597619	2.1:-63596948	MS.gene047314:CDS
GAGAAGAGGAAAACAATCAA+CGG	0.615125	2.1:-63597482	MS.gene047314:intron
GTACAGTGAAACATTATGGA+TGG	0.625851	2.1:-63596951	MS.gene047314:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTGCAACAATATATCTA+GGG	+	chr2.1:63597631-63597650	None:intergenic	20.0%
!!	TAAATTGCAACAATATATCT+AGG	+	chr2.1:63597632-63597651	None:intergenic	20.0%
!	ATTTCTTTCGGTTTAATCAT+AGG	+	chr2.1:63597530-63597549	None:intergenic	25.0%
!	TTACTCAAATAAATCTTGAG+TGG	+	chr2.1:63597213-63597232	None:intergenic	25.0%
!!!	GCAATTTAATTTGTTGTTGA+GGG	-	chr2.1:63597644-63597663	MS.gene047314:CDS	25.0%
!!!	TATTCTTTAAGATTGCAACA+AGG	+	chr2.1:63597496-63597515	None:intergenic	25.0%
!!!	TGCAATTTAATTTGTTGTTG+AGG	-	chr2.1:63597643-63597662	MS.gene047314:CDS	25.0%
	ATCATCATCATCATAGAAGA+TGG	+	chr2.1:63597345-63597364	None:intergenic	30.0%
	GACATAATATGTATGGATCT+GGG	-	chr2.1:63597367-63597386	MS.gene047314:intron	30.0%
	TTGCTCATTATGATCTTATC+TGG	+	chr2.1:63597448-63597467	None:intergenic	30.0%
	TTTGAGTAAGTATAGAGAGA+TGG	-	chr2.1:63597224-63597243	MS.gene047314:intron	30.0%
!	ATCTTCTATGATGATGATGA+TGG	-	chr2.1:63597344-63597363	MS.gene047314:intron	30.0%
!	ATGATGGGACATAATATGTA+TGG	-	chr2.1:63597360-63597379	MS.gene047314:intron	30.0%
!	TCTTCTATGATGATGATGAT+GGG	-	chr2.1:63597345-63597364	MS.gene047314:intron	30.0%
	AGTAAGTATAGAGAGATGGA+AGG	-	chr2.1:63597228-63597247	MS.gene047314:intron	35.0%
	ATAAGTGTCAGAAAGAGAAG+AGG	-	chr2.1:63597121-63597140	MS.gene047314:intron	35.0%
	GAGAAGAGGAAAACAATCAA+CGG	-	chr2.1:63597135-63597154	MS.gene047314:intron	35.0%
	GAGATGACTCGAATTTCTTT+CGG	+	chr2.1:63597542-63597561	None:intergenic	35.0%
	GAGTTCATCAGTTTCATAAC+AGG	-	chr2.1:63597087-63597106	MS.gene047314:intron	35.0%
	GGACATAATATGTATGGATC+TGG	-	chr2.1:63597366-63597385	MS.gene047314:intron	35.0%
	GTACAGTGAAACATTATGGA+TGG	-	chr2.1:63597666-63597685	MS.gene047314:CDS	35.0%
!	AAACATTATGGATGGTGGTT+TGG	-	chr2.1:63597674-63597693	MS.gene047314:CDS	35.0%
	AATATGTATGGATCTGGGAG+TGG	-	chr2.1:63597372-63597391	MS.gene047314:intron	40.0%
	CAACGGTGATGATCTTCTTT+GGG	-	chr2.1:63597152-63597171	MS.gene047314:intron	40.0%
	CAGTGAAACATTATGGATGG+TGG	-	chr2.1:63597669-63597688	MS.gene047314:CDS	40.0%
	CGTGAGTAGGATCATGAAAA+AGG	-	chr2.1:63597002-63597021	MS.gene047314:intron	40.0%
	TCAACGGTGATGATCTTCTT+TGG	-	chr2.1:63597151-63597170	MS.gene047314:intron	40.0%
	TTCCAATAGCAAACGTGAGT+AGG	-	chr2.1:63596989-63597008	MS.gene047314:intron	40.0%
!	GAGGGTACAGTGAAACATTA+TGG	-	chr2.1:63597662-63597681	MS.gene047314:CDS	40.0%
!	TTTGAGATCTTTGCGTTTGC+TGG	+	chr2.1:63597033-63597052	None:intergenic	40.0%
!!	AGAAGAGTGCTATGATTGGT+AGG	-	chr2.1:63597253-63597272	MS.gene047314:intron	40.0%
!!	ATCCTACTCACGTTTGCTAT+TGG	+	chr2.1:63596994-63597013	None:intergenic	40.0%
	CTTTGGGCTATGACAACTCT+TGG	-	chr2.1:63597168-63597187	MS.gene047314:intron	45.0%
	GAATCAGACAATGAGTCAGC+AGG	-	chr2.1:63596943-63596962	MS.gene047314:CDS	45.0%
	GATCAGAGAGATGGGAATGT+TGG	-	chr2.1:63597279-63597298	MS.gene047314:intron	45.0%
	GGAGTTTATGGTCATGGAGT+TGG	-	chr2.1:63597315-63597334	MS.gene047314:intron	45.0%
	GTAGGAGTGATCAGAGAGAT+GGG	-	chr2.1:63597271-63597290	MS.gene047314:intron	45.0%
!	AGAGATGGGAATGTTGGTGA+GGG	-	chr2.1:63597285-63597304	MS.gene047314:intron	45.0%
!	AGTGGTGGAGTTTATGGTCA+TGG	-	chr2.1:63597309-63597328	MS.gene047314:intron	45.0%
!	TCTTATCTGGCTCGAGAGAT+TGG	+	chr2.1:63597435-63597454	None:intergenic	45.0%
!!	GGAGAGAAGAGTGCTATGAT+TGG	-	chr2.1:63597249-63597268	MS.gene047314:intron	45.0%
	CAGGTTGCAGAGAGCAAGAT+AGG	-	chr2.1:63596962-63596981	MS.gene047314:intron	50.0%
	CGAGTACTCCTTCCAGAAGA+TGG	+	chr2.1:63597402-63597421	None:intergenic	50.0%
	GAGAGATGGGAATGTTGGTG+AGG	-	chr2.1:63597284-63597303	MS.gene047314:intron	50.0%
	GGTAGGAGTGATCAGAGAGA+TGG	-	chr2.1:63597270-63597289	MS.gene047314:intron	50.0%
!	AATGTTGGTGAGGGTAGTGG+TGG	-	chr2.1:63597294-63597313	MS.gene047314:intron	50.0%
!	GAGGGTAGTGGTGGAGTTTA+TGG	-	chr2.1:63597303-63597322	MS.gene047314:intron	50.0%
	GTGGATCGCCATCTTCTGGA+AGG	-	chr2.1:63597391-63597410	MS.gene047314:intron	55.0%
!	GGGAATGTTGGTGAGGGTAG+TGG	-	chr2.1:63597291-63597310	MS.gene047314:intron	55.0%
	GGGAGTGGATCGCCATCTTC+TGG	-	chr2.1:63597387-63597406	MS.gene047314:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	63596937	63597702	63596937	ID=MS.gene047314
chr2.1	mRNA	63596937	63597702	63596937	ID=MS.gene047314.t1;Parent=MS.gene047314
chr2.1	exon	63597483	63597702	63597483	ID=MS.gene047314.t1.exon1;Parent=MS.gene047314.t1
chr2.1	CDS	63597483	63597702	63597483	ID=cds.MS.gene047314.t1;Parent=MS.gene047314.t1
chr2.1	exon	63596937	63596974	63596937	ID=MS.gene047314.t1.exon2;Parent=MS.gene047314.t1
chr2.1	CDS	63596937	63596974	63596937	ID=cds.MS.gene047314.t1;Parent=MS.gene047314.t1

Gene Sequence

>MS.gene047314

ATGGCTGAATCAGACAATGAGTCAGCAGGTTGCAGAGAGCAAGATAGGTTTCTTCCAATAGCAAACGTGAGTAGGATCATGAAAAAGGCTTTACCAGCAAACGCAAAGATCTCAAAAGAAGCAAAAGAAACAGTGCAAGAGTGTGTATCAGAGTTCATCAGTTTCATAACAGGAGAAGCTTCTGATAAGTGTCAGAAAGAGAAGAGGAAAACAATCAACGGTGATGATCTTCTTTGGGCTATGACAACTCTTGGATTTGAAGATTATGTTGAGCCACTCAAGATTTATTTGAGTAAGTATAGAGAGATGGAAGGAGAGAAGAGTGCTATGATTGGTAGGAGTGATCAGAGAGATGGGAATGTTGGTGAGGGTAGTGGTGGAGTTTATGGTCATGGAGTTGGAGTTCCATCTTCTATGATGATGATGATGGGACATAATATGTATGGATCTGGGAGTGGATCGCCATCTTCTGGAAGGAGTACTCGATAGCAGAGACCAATCTCTCGAGCCAGATAAGATCATAATGAGCAAGAAACTTCTGTCTTAAGAATTTTAACCTTGTTGCAATCTTAAAGAATACAAACTTAAGACCTATGATTAAACCGAAAGAAATTCGAGTCATCTCATCTAGACAACAAGTTTATGTATGCATATGTTTGATATAAATTCATGTATTTTTTAATTTAGAGTACCCTAGATATATTGTTGCAATTTAATTTGTTGTTGAGGGTACAGTGAAACATTATGGATGGTGGTTTGGAATTAA

Protein sequence

>MS.gene047314.t1

MAESDNESAGCREQDRFLPIANVSRIMKKALPANAKISKEAKETVQECVSEFISFITGEASDKCQKEKRKTINGYSETLWMVVWN