AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047374

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047374.t1 MTR_4g088125 73.830 470 108 3 3 461 5 470 0.0 712
MS.gene047374.t1 MTR_4g079240 45.783 498 201 10 3 437 6 497 5.40e-137 404
MS.gene047374.t1 MTR_2g014870 92.913 127 6 2 1 124 1 127 7.89e-75 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047374.t1 AT1G28520 64.807 466 139 6 3 450 6 464 0.0 589
MS.gene047374.t1 AT1G28520 64.807 466 139 6 3 450 6 464 0.0 589
MS.gene047374.t1 AT1G28520 64.807 466 139 6 3 450 6 464 0.0 589
MS.gene047374.t1 AT1G28520 64.807 466 139 6 3 450 6 464 0.0 589
MS.gene047374.t1 AT1G28520 62.017 466 136 7 3 450 6 448 0.0 551
MS.gene047374.t1 AT2G42400 49.091 440 177 10 10 416 9 434 4.43e-124 369

Find 113 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCATCACTTGCTTTC+TGG -0.000768 2.3:+70130436 None:intergenic
GTTGCCGCTCCCTCGCATTC+TGG 0.120554 2.3:+70130715 None:intergenic
GTATGTACTCATCCATTAAT+AGG 0.244748 2.3:+70131157 None:intergenic
TTGGAACTAAAGCTCGTTAA+TGG 0.245316 2.3:-70130302 MS.gene047374:CDS
TCAGCATGATTCCCTATTAA+TGG 0.250839 2.3:-70131169 MS.gene047374:CDS
CATAAAGATGCCACTCAAAA+TGG 0.277276 2.3:+70130366 None:intergenic
CGAGTTGTTAGTTGATTTAC+TGG 0.280780 2.3:+70131116 None:intergenic
TTGCCGCTCCCTCGCATTCT+GGG 0.309111 2.3:+70130716 None:intergenic
AGGTCGTTGCCTGATTATAA+TGG 0.319828 2.3:-70130476 MS.gene047374:CDS
GGTAGATAGGTAGGTATATA+AGG 0.325810 2.3:+70130979 None:intergenic
GTCAGCCTTGGCATTAGGTT+TGG 0.334729 2.3:+70130163 None:intergenic
GTCTGCATATTCTGATCATT+AGG 0.347297 2.3:+70131694 None:intergenic
GAGTACATACACTCCATCTT+AGG 0.353453 2.3:-70131144 MS.gene047374:CDS
CCCATGTCAGCCTTGGCATT+AGG 0.366697 2.3:+70130158 None:intergenic
AGGGCAGATGCTTGGTAGAT+AGG 0.368518 2.3:+70130966 None:intergenic
AAGCAAGTGATGAAAGAGTT+TGG 0.371141 2.3:-70130431 MS.gene047374:CDS
TCCTGCTTCTTCTTTGCAGC+AGG 0.376005 2.3:-70131955 MS.gene047374:intron
ATCATTTGTTATCTTTGTCC+TGG 0.382306 2.3:+70130268 None:intergenic
AGCAGAATGTCTGTTATCAT+AGG 0.386907 2.3:+70130196 None:intergenic
AGGTTCAAAATGTGCACTTT+GGG 0.394325 2.3:-70130926 MS.gene047374:CDS
CCTCCTCCTTCCGCTTTCCT+AGG 0.410735 2.3:-70130946 MS.gene047374:CDS
TGCCACTCAAAATGGTTTAG+TGG 0.430399 2.3:+70130374 None:intergenic
TCAAAATGGTTTAGTGGTTG+TGG 0.432899 2.3:+70130380 None:intergenic
TCATGCTTAGGCACAGCTAA+TGG 0.436229 2.3:+70131718 None:intergenic
TAGGTTCAAAATGTGCACTT+TGG 0.438695 2.3:-70130927 MS.gene047374:CDS
GTGATGCATGTGCGTTATAT+CGG 0.439847 2.3:-70130325 MS.gene047374:CDS
CTGCAGTAGCCTACATATAT+CGG 0.447566 2.3:+70131771 None:intergenic
GGTCGTTGCCTGATTATAAT+GGG 0.452127 2.3:-70130475 MS.gene047374:CDS
CCATCCACTCACGAAGCATC+TGG 0.454510 2.3:+70131997 None:intergenic
TATGTACTCATCCATTAATA+GGG 0.456560 2.3:+70131158 None:intergenic
TGGAACTAAAGCTCGTTAAT+GGG 0.459138 2.3:-70130301 MS.gene047374:CDS
GCTGCTCTTGCTTTAAATGA+AGG 0.460368 2.3:-70130844 MS.gene047374:CDS
CATCCCCGCCCATTATAATC+AGG 0.465616 2.3:+70130467 None:intergenic
TGATCATTAGGTTCATGCTT+AGG 0.466635 2.3:+70131706 None:intergenic
TAGCCCCAGTTCTACGACCT+GGG 0.466781 2.3:-70130810 MS.gene047374:CDS
TGCTTTCTTACAGCTACCCT+TGG 0.466878 2.3:+70132148 None:intergenic
ACCTAATGCCAAGGCTGACA+TGG 0.476238 2.3:-70130159 MS.gene047374:CDS
CGTAGAACTGGGGCTATGCC+TGG 0.483033 2.3:+70130817 None:intergenic
TGATCTGCAGTTTGCTAGGA+AGG 0.490071 2.3:-70132063 MS.gene047374:CDS
CTGATTATAATGGGCGGGGA+TGG 0.493655 2.3:-70130466 MS.gene047374:CDS
TCCTAGCAAACTGCAGATCA+AGG 0.504979 2.3:+70132066 None:intergenic
GTTGCCTGATTATAATGGGC+GGG 0.508969 2.3:-70130471 MS.gene047374:CDS
TGGTTGTGGATCCATGTAGT+AGG 0.510429 2.3:+70130394 None:intergenic
CATCCCAGAATGCGAGGGAG+CGG 0.511232 2.3:-70130719 MS.gene047374:CDS
TTTACTGGGAGCTCCTAAGA+TGG 0.517698 2.3:+70131131 None:intergenic
CCAGATGCTTCGTGAGTGGA+TGG 0.518587 2.3:-70131997 MS.gene047374:CDS
AACACCACCCATGTCAGCCT+TGG 0.521369 2.3:+70130151 None:intergenic
CCTAGGAAAGCGGAAGGAGG+AGG 0.522377 2.3:+70130946 None:intergenic
GAAGGAGGAGGGCAGATGCT+TGG 0.522630 2.3:+70130958 None:intergenic
CTCTCAACAAGATAGTCATA+TGG 0.523925 2.3:+70130101 None:intergenic
TGTGGATGTCGCGCTTCTTG+AGG 0.525115 2.3:-70132030 MS.gene047374:CDS
AGCATCACACAGGCTTATCA+AGG 0.532332 2.3:-70132129 MS.gene047374:CDS
CACAAGTTCAACCCTTCCGC+GGG 0.534065 2.3:+70130892 None:intergenic
TGATAACAGACATTCTGCTA+AGG 0.543724 2.3:-70130192 MS.gene047374:CDS
GGCACAGGCTTTAGTGAGGA+TGG 0.545649 2.3:-70131009 MS.gene047374:CDS
ACCTGCTGCAAAGAAGAAGC+AGG 0.547011 2.3:+70131954 None:intergenic
CATTCTCGACCGATATATGT+AGG 0.550090 2.3:-70131780 MS.gene047374:CDS
CAGATGCTTGGTAGATAGGT+AGG 0.551289 2.3:+70130970 None:intergenic
GCTTTAAATGAAGGACAACC+AGG 0.554170 2.3:-70130835 MS.gene047374:CDS
GCAGAATGTCTGTTATCATA+GGG 0.554543 2.3:+70130197 None:intergenic
AGTTAAGGATAATTTACTGT+TGG 0.554729 2.3:-70130779 MS.gene047374:CDS
ATAGCCCCAGTTCTACGACC+TGG 0.557433 2.3:-70130811 MS.gene047374:CDS
TGAGGTTTAGATGATTTCCA+AGG 0.559690 2.3:-70132165 None:intergenic
ATAGGGAATCATGCTGATGT+TGG 0.560720 2.3:+70131175 None:intergenic
TTAATGGGAAGAAGAGCTCC+AGG 0.561240 2.3:-70130286 MS.gene047374:CDS
TCCTTGATCTGCAGTTTGCT+AGG 0.561339 2.3:-70132067 MS.gene047374:CDS
AGGAGAAGGCTCATTGAGCT+CGG 0.567463 2.3:+70131974 None:intergenic
CAGTAAATCAACTAACAACT+CGG 0.567663 2.3:-70131115 MS.gene047374:CDS
ACACAAGTTCAACCCTTCCG+CGG 0.569071 2.3:+70130891 None:intergenic
TGCAAAGAAGAAGCAGGAGA+AGG 0.571409 2.3:+70131960 None:intergenic
AAGTCTCCGTGGAATGCACC+TGG 0.578340 2.3:-70130688 MS.gene047374:intron
CTAGGAAAGCGGAAGGAGGA+GGG 0.578498 2.3:+70130947 None:intergenic
TTGCCTGATTATAATGGGCG+GGG 0.584990 2.3:-70130470 MS.gene047374:CDS
AAGTGATGAAAGAGTTTGGA+GGG 0.587404 2.3:-70130427 MS.gene047374:CDS
GACTGTTCAAGACCCGCGGA+AGG 0.587498 2.3:-70130904 MS.gene047374:CDS
GCTCTTAGTGCAAAGACAGA+AGG 0.588265 2.3:-70130754 MS.gene047374:CDS
GATAACAGACATTCTGCTAA+GGG 0.588460 2.3:-70130191 MS.gene047374:CDS
ACAAGATAGTCATATGGTGA+TGG 0.593941 2.3:+70130107 None:intergenic
CAGTTACTGATCTTCAGAAG+CGG 0.596139 2.3:-70130244 MS.gene047374:CDS
TTCACCAGATGCTTCGTGAG+TGG 0.596799 2.3:-70132001 MS.gene047374:CDS
TGCATGTGCGTTATATCGGT+TGG 0.607322 2.3:-70130321 MS.gene047374:CDS
CAGAGCCAAACCTAATGCCA+AGG 0.608334 2.3:-70130168 MS.gene047374:CDS
AATCAACTAACAACTCGGAT+AGG 0.609346 2.3:-70131110 MS.gene047374:CDS
AGCCCCAGTTCTACGACCTG+GGG 0.611570 2.3:-70130809 MS.gene047374:CDS
CCTAATGCCAAGGCTGACAT+GGG 0.613097 2.3:-70130158 MS.gene047374:CDS
CTAATACCAGGTGCATTCCA+CGG 0.614138 2.3:+70130682 None:intergenic
TGTCGGCATCCCAGAATGCG+AGG 0.614404 2.3:-70130725 MS.gene047374:CDS
GCTACTGCAGCTCTGTGAAG+AGG 0.620067 2.3:-70131758 MS.gene047374:CDS
AAGACAGAAGGAAAAGATGT+CGG 0.620071 2.3:-70130742 MS.gene047374:CDS
GAGGTTTAGATGATTTCCAA+GGG 0.620877 2.3:-70132164 None:intergenic
AGGCACAGCTAATGGACTAG+TGG 0.628612 2.3:+70131726 None:intergenic
CGTTGCCTGATTATAATGGG+CGG 0.632470 2.3:-70130472 MS.gene047374:CDS
TAGAGGCACAGGCTTTAGTG+AGG 0.634083 2.3:-70131013 MS.gene047374:CDS
TTTGAACCTAGGAAAGCGGA+AGG 0.634334 2.3:+70130940 None:intergenic
GCTGAAGAGATCCTACTACA+TGG 0.634906 2.3:-70130405 MS.gene047374:CDS
CAACTAACAACTCGGATAGG+CGG 0.636833 2.3:-70131107 MS.gene047374:CDS
TCTCGAAGAGCATTTGAGAG+TGG 0.636901 2.3:-70130512 MS.gene047374:CDS
ACTGTTCAAGACCCGCGGAA+GGG 0.637959 2.3:-70130903 MS.gene047374:CDS
TTGGGACTGTTCAAGACCCG+CGG 0.641938 2.3:-70130908 MS.gene047374:CDS
GAAGCGCGACATCCACACTA+CGG 0.643778 2.3:+70132036 None:intergenic
TCACATGCAGATCATCTACA+TGG 0.644881 2.3:+70132093 None:intergenic
GAACCTAGGAAAGCGGAAGG+AGG 0.646171 2.3:+70130943 None:intergenic
TCTGTTATCATAGGGAAACT+CGG 0.647001 2.3:+70130205 None:intergenic
CGGCAACTGCAAAGTCTCCG+TGG 0.652685 2.3:-70130699 MS.gene047374:CDS
GTCGGCATCCCAGAATGCGA+GGG 0.657248 2.3:-70130724 MS.gene047374:CDS
TAGGAAGGAGAGCCGTAGTG+TGG 0.661628 2.3:-70132048 MS.gene047374:CDS
GAGTTGTTAGTTGATTTACT+GGG 0.663415 2.3:+70131117 None:intergenic
CAAGTGATGAAAGAGTTTGG+AGG 0.680936 2.3:-70130428 MS.gene047374:CDS
GCTGTAAGAAAGCATCACAC+AGG 0.691286 2.3:-70132139 MS.gene047374:CDS
ACACAGGCTTATCAAGGACA+AGG 0.694542 2.3:-70132123 MS.gene047374:CDS
AGAGTGGAAACAGAAAGCAA+AGG 0.717117 2.3:-70130496 MS.gene047374:CDS
GCCCCAGTTCTACGACCTGG+GGG 0.725582 2.3:-70130808 MS.gene047374:CDS
AATGCCAAGGCTGACATGGG+TGG 0.739584 2.3:-70130155 MS.gene047374:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATTTTTTAGAGAAA+AGG + chr2.3:70130837-70130856 None:intergenic 10.0%
!! AAACACATAAATAGTTTAAT+AGG + chr2.3:70131601-70131620 None:intergenic 15.0%
!!! TTTTATTTTTTCTGTAGTAT+TGG - chr2.3:70130658-70130677 MS.gene047374:intron 15.0%
!! AAATCTGCATATTAAAGTTA+AGG + chr2.3:70130326-70130345 None:intergenic 20.0%
!! TAATTCATATACTAGAGTAA+GGG - chr2.3:70130729-70130748 MS.gene047374:CDS 20.0%
!! TTAATATGCAGATTTAATTG+TGG - chr2.3:70130331-70130350 MS.gene047374:CDS 20.0%
!! TTAATTCATATACTAGAGTA+AGG - chr2.3:70130728-70130747 MS.gene047374:CDS 20.0%
!!! GTTGTATTGTTTTTTATATG+TGG - chr2.3:70130808-70130827 MS.gene047374:CDS 20.0%
!!! TTATTTTCAATTGACAGAAA+TGG - chr2.3:70130864-70130883 MS.gene047374:CDS 20.0%
! AGTTAAGGATAATTTACTGT+TGG - chr2.3:70131458-70131477 MS.gene047374:intron 25.0%
! ATGATGCATAAGAACAAATT+GGG + chr2.3:70130956-70130975 None:intergenic 25.0%
! ATTTAATTGTGGATTTGTGA+TGG - chr2.3:70130342-70130361 MS.gene047374:CDS 25.0%
! GAGAAAATTGGTATTAACTA+AGG + chr2.3:70130918-70130937 None:intergenic 25.0%
! TATGATGCATAAGAACAAAT+TGG + chr2.3:70130957-70130976 None:intergenic 25.0%
! TATGATTGTAACAAGTTTCT+TGG - chr2.3:70131021-70131040 MS.gene047374:CDS 25.0%
! TATGTACTCATCCATTAATA+GGG + chr2.3:70131082-70131101 None:intergenic 25.0%
!! TTTGATCTTTTTGTTCTTGA+GGG - chr2.3:70131668-70131687 MS.gene047374:intron 25.0%
!!! CGACATTTTGATTCAAAATT+AGG + chr2.3:70131156-70131175 None:intergenic 25.0%
AACTTGAATGGCAATATTTG+TGG - chr2.3:70130374-70130393 MS.gene047374:CDS 30.0%
CAGTAAATCAACTAACAACT+CGG - chr2.3:70131122-70131141 MS.gene047374:CDS 30.0%
CTTTGTGATTTGATCATAAC+TGG - chr2.3:70130629-70130648 MS.gene047374:intron 30.0%
GTATGTACTCATCCATTAAT+AGG + chr2.3:70131083-70131102 None:intergenic 30.0%
! ATTTGAGTTTGCCATGTTTA+TGG - chr2.3:70130408-70130427 MS.gene047374:CDS 30.0%
! CTTTGATCTTTTTGTTCTTG+AGG - chr2.3:70131667-70131686 MS.gene047374:intron 30.0%
! GAGTTGTTAGTTGATTTACT+GGG + chr2.3:70131123-70131142 None:intergenic 30.0%
! TTGATCTTTTTGTTCTTGAG+GGG - chr2.3:70131669-70131688 MS.gene047374:CDS 30.0%
! TTTGAGTTTGCCATGTTTAT+GGG - chr2.3:70130409-70130428 MS.gene047374:CDS 30.0%
!! AACTGTTATACTGCTTTTAG+AGG - chr2.3:70131207-70131226 MS.gene047374:intron 30.0%
!! ATCATTTGTTATCTTTGTCC+TGG + chr2.3:70131972-70131991 None:intergenic 30.0%
AAGACAGAAGGAAAAGATGT+CGG - chr2.3:70131495-70131514 MS.gene047374:intron 35.0%
AAGCAAGTGATGAAAGAGTT+TGG - chr2.3:70131806-70131825 MS.gene047374:intron 35.0%
AAGTGATGAAAGAGTTTGGA+GGG - chr2.3:70131810-70131829 MS.gene047374:intron 35.0%
AATCAACTAACAACTCGGAT+AGG - chr2.3:70131127-70131146 MS.gene047374:CDS 35.0%
ACAAGATAGTCATATGGTGA+TGG + chr2.3:70132133-70132152 None:intergenic 35.0%
AGCAGAATGTCTGTTATCAT+AGG + chr2.3:70132044-70132063 None:intergenic 35.0%
AGGTTCAAAATGTGCACTTT+GGG - chr2.3:70131311-70131330 MS.gene047374:intron 35.0%
CATAAAGATGCCACTCAAAA+TGG + chr2.3:70131874-70131893 None:intergenic 35.0%
CATGTTTATGGGTGATGATA+GGG - chr2.3:70130420-70130439 MS.gene047374:CDS 35.0%
CTCTCAACAAGATAGTCATA+TGG + chr2.3:70132139-70132158 None:intergenic 35.0%
GATAACAGACATTCTGCTAA+GGG - chr2.3:70132046-70132065 MS.gene047374:CDS 35.0%
GCAGAATGTCTGTTATCATA+GGG + chr2.3:70132043-70132062 None:intergenic 35.0%
GCATTCTACATTCTAATACC+AGG + chr2.3:70131570-70131589 None:intergenic 35.0%
GGTAGATAGGTAGGTATATA+AGG + chr2.3:70131261-70131280 None:intergenic 35.0%
GTCTGCATATTCTGATCATT+AGG + chr2.3:70130546-70130565 None:intergenic 35.0%
TAGGTTCAAAATGTGCACTT+TGG - chr2.3:70131310-70131329 MS.gene047374:intron 35.0%
TCAAAATGGTTTAGTGGTTG+TGG + chr2.3:70131860-70131879 None:intergenic 35.0%
TCAGCATGATTCCCTATTAA+TGG - chr2.3:70131068-70131087 MS.gene047374:CDS 35.0%
TCTGTTATCATAGGGAAACT+CGG + chr2.3:70132035-70132054 None:intergenic 35.0%
TGATAACAGACATTCTGCTA+AGG - chr2.3:70132045-70132064 MS.gene047374:CDS 35.0%
TGATCATTAGGTTCATGCTT+AGG + chr2.3:70130534-70130553 None:intergenic 35.0%
TGGAACTAAAGCTCGTTAAT+GGG - chr2.3:70131936-70131955 MS.gene047374:intron 35.0%
TGGACACATGTAAACTTGAA+TGG - chr2.3:70130362-70130381 MS.gene047374:CDS 35.0%
TGTATGAATAATCGCTGAAG+AGG + chr2.3:70130608-70130627 None:intergenic 35.0%
TTGGAACTAAAGCTCGTTAA+TGG - chr2.3:70131935-70131954 MS.gene047374:intron 35.0%
! AAAGTGCACATTTTGAACCT+AGG + chr2.3:70131311-70131330 None:intergenic 35.0%
! AACCACTAAACCATTTTGAG+TGG - chr2.3:70131861-70131880 MS.gene047374:intron 35.0%
! ACATTTTGAACCTAGGAAAG+CGG + chr2.3:70131304-70131323 None:intergenic 35.0%
! CGAGTTGTTAGTTGATTTAC+TGG + chr2.3:70131124-70131143 None:intergenic 35.0%
! TGAGTTGTTGGTGAGAAAAT+TGG + chr2.3:70130930-70130949 None:intergenic 35.0%
! TGATCTTTTTGTTCTTGAGG+GGG - chr2.3:70131670-70131689 MS.gene047374:CDS 35.0%
AATGGAGAGCAAGGATTTGA+TGG - chr2.3:70130882-70130901 MS.gene047374:CDS 40.0%
AGAGTGGAAACAGAAAGCAA+AGG - chr2.3:70131741-70131760 MS.gene047374:CDS 40.0%
AGGTCGTTGCCTGATTATAA+TGG - chr2.3:70131761-70131780 MS.gene047374:CDS 40.0%
ATAATCGCTGAAGAGGAGAA+AGG + chr2.3:70130601-70130620 None:intergenic 40.0%
ATAGGGAATCATGCTGATGT+TGG + chr2.3:70131065-70131084 None:intergenic 40.0%
ATTGACAGAAATGGAGAGCA+AGG - chr2.3:70130873-70130892 MS.gene047374:CDS 40.0%
CAAGTGATGAAAGAGTTTGG+AGG - chr2.3:70131809-70131828 MS.gene047374:intron 40.0%
CAGTTACTGATCTTCAGAAG+CGG - chr2.3:70131993-70132012 MS.gene047374:CDS 40.0%
CATTCTCGACCGATATATGT+AGG - chr2.3:70130457-70130476 MS.gene047374:CDS 40.0%
CCATGTTTATGGGTGATGAT+AGG - chr2.3:70130419-70130438 MS.gene047374:CDS 40.0%
CCTATCATCACCCATAAACA+TGG + chr2.3:70130422-70130441 None:intergenic 40.0%
CTCTTTCATCACTTGCTTTC+TGG + chr2.3:70131804-70131823 None:intergenic 40.0%
CTGCAGTAGCCTACATATAT+CGG + chr2.3:70130469-70130488 None:intergenic 40.0%
GAGTACATACACTCCATCTT+AGG - chr2.3:70131093-70131112 MS.gene047374:CDS 40.0%
GGTCGTTGCCTGATTATAAT+GGG - chr2.3:70131762-70131781 MS.gene047374:CDS 40.0%
GTGATGCATGTGCGTTATAT+CGG - chr2.3:70131912-70131931 MS.gene047374:intron 40.0%
TACTAGAGTAAGGGATGCTT+TGG - chr2.3:70130738-70130757 MS.gene047374:CDS 40.0%
TCACATGCAGATCATCTACA+TGG + chr2.3:70130147-70130166 None:intergenic 40.0%
TGCCACTCAAAATGGTTTAG+TGG + chr2.3:70131866-70131885 None:intergenic 40.0%
! GCTGCTCTTGCTTTAAATGA+AGG - chr2.3:70131393-70131412 MS.gene047374:intron 40.0%
! GCTTTAAATGAAGGACAACC+AGG - chr2.3:70131402-70131421 MS.gene047374:intron 40.0%
!! CAAATTGGGCTTTGAGTTGT+TGG + chr2.3:70130942-70130961 None:intergenic 40.0%
!! TATACTGCTTTTAGAGGCAC+AGG - chr2.3:70131213-70131232 MS.gene047374:intron 40.0%
ACACAGGCTTATCAAGGACA+AGG - chr2.3:70130114-70130133 MS.gene047374:CDS 45.0%
ACCTGGGGGAATTGAAGTTA+AGG - chr2.3:70131443-70131462 MS.gene047374:intron 45.0%
AGCATCACACAGGCTTATCA+AGG - chr2.3:70130108-70130127 MS.gene047374:CDS 45.0%
CAACTAACAACTCGGATAGG+CGG - chr2.3:70131130-70131149 MS.gene047374:CDS 45.0%
CAGATGCTTGGTAGATAGGT+AGG + chr2.3:70131270-70131289 None:intergenic 45.0%
CTAATACCAGGTGCATTCCA+CGG + chr2.3:70131558-70131577 None:intergenic 45.0%
GCTCTTAGTGCAAAGACAGA+AGG - chr2.3:70131483-70131502 MS.gene047374:intron 45.0%
GCTGAAGAGATCCTACTACA+TGG - chr2.3:70131832-70131851 MS.gene047374:intron 45.0%
GTTTATGGGTGATGATAGGG+TGG - chr2.3:70130423-70130442 MS.gene047374:CDS 45.0%
TCATGCTTAGGCACAGCTAA+TGG + chr2.3:70130522-70130541 None:intergenic 45.0%
TCCTAGCAAACTGCAGATCA+AGG + chr2.3:70130174-70130193 None:intergenic 45.0%
TCCTTAACTTCAATTCCCCC+AGG + chr2.3:70131447-70131466 None:intergenic 45.0%
TCTTGAGGGGGAGACTATTA+GGG - chr2.3:70131682-70131701 MS.gene047374:CDS 45.0%
TGCAAAGAAGAAGCAGGAGA+AGG + chr2.3:70130280-70130299 None:intergenic 45.0%
TGCATGTGCGTTATATCGGT+TGG - chr2.3:70131916-70131935 MS.gene047374:intron 45.0%
TGCTTTCTTACAGCTACCCT+TGG + chr2.3:70130092-70130111 None:intergenic 45.0%
TGGTTGTGGATCCATGTAGT+AGG + chr2.3:70131846-70131865 None:intergenic 45.0%
TTAATGGGAAGAAGAGCTCC+AGG - chr2.3:70131951-70131970 MS.gene047374:intron 45.0%
TTCTTGAGGGGGAGACTATT+AGG - chr2.3:70131681-70131700 MS.gene047374:CDS 45.0%
TTTACTGGGAGCTCCTAAGA+TGG + chr2.3:70131109-70131128 None:intergenic 45.0%
TTTGAACCTAGGAAAGCGGA+AGG + chr2.3:70131300-70131319 None:intergenic 45.0%
! CGTTGCCTGATTATAATGGG+CGG - chr2.3:70131765-70131784 MS.gene047374:CDS 45.0%
! TCCTTGATCTGCAGTTTGCT+AGG - chr2.3:70130170-70130189 MS.gene047374:CDS 45.0%
!! AATCATGCTGATGTTGGCTC+TGG + chr2.3:70131059-70131078 None:intergenic 45.0%
!! GCTGTAAGAAAGCATCACAC+AGG - chr2.3:70130098-70130117 MS.gene047374:CDS 45.0%
!! GTTGCCTGATTATAATGGGC+GGG - chr2.3:70131766-70131785 MS.gene047374:CDS 45.0%
!! TCTCGAAGAGCATTTGAGAG+TGG - chr2.3:70131725-70131744 MS.gene047374:CDS 45.0%
!! TGATCTGCAGTTTGCTAGGA+AGG - chr2.3:70130174-70130193 MS.gene047374:CDS 45.0%
!! TTGCCTGATTATAATGGGCG+GGG - chr2.3:70131767-70131786 MS.gene047374:CDS 45.0%
ACACAAGTTCAACCCTTCCG+CGG + chr2.3:70131349-70131368 None:intergenic 50.0%
ACCTAATGCCAAGGCTGACA+TGG - chr2.3:70132078-70132097 MS.gene047374:CDS 50.0%
ACCTGCTGCAAAGAAGAAGC+AGG + chr2.3:70130286-70130305 None:intergenic 50.0%
AGGAGAAGGCTCATTGAGCT+CGG + chr2.3:70130266-70130285 None:intergenic 50.0%
AGGGCAGATGCTTGGTAGAT+AGG + chr2.3:70131274-70131293 None:intergenic 50.0%
CAGAGCCAAACCTAATGCCA+AGG - chr2.3:70132069-70132088 MS.gene047374:CDS 50.0%
CATCCCCGCCCATTATAATC+AGG + chr2.3:70131773-70131792 None:intergenic 50.0%
CCTAATGCCAAGGCTGACAT+GGG - chr2.3:70132079-70132098 MS.gene047374:CDS 50.0%
GTCAGCCTTGGCATTAGGTT+TGG + chr2.3:70132077-70132096 None:intergenic 50.0%
TCCTGCTTCTTCTTTGCAGC+AGG - chr2.3:70130282-70130301 MS.gene047374:CDS 50.0%
TTCACCAGATGCTTCGTGAG+TGG - chr2.3:70130236-70130255 MS.gene047374:CDS 50.0%
! TAGAGGCACAGGCTTTAGTG+AGG - chr2.3:70131224-70131243 MS.gene047374:intron 50.0%
!! AGGCACAGCTAATGGACTAG+TGG + chr2.3:70130514-70130533 None:intergenic 50.0%
!! CTGATTATAATGGGCGGGGA+TGG - chr2.3:70131771-70131790 MS.gene047374:CDS 50.0%
AACACCACCCATGTCAGCCT+TGG + chr2.3:70132089-70132108 None:intergenic 55.0%
AAGTCTCCGTGGAATGCACC+TGG - chr2.3:70131549-70131568 MS.gene047374:intron 55.0%
AATGCCAAGGCTGACATGGG+TGG - chr2.3:70132082-70132101 MS.gene047374:CDS 55.0%
ACTGTTCAAGACCCGCGGAA+GGG - chr2.3:70131334-70131353 MS.gene047374:intron 55.0%
AGGGGGAGACTATTAGGGAG+TGG - chr2.3:70131687-70131706 MS.gene047374:CDS 55.0%
ATAGCCCCAGTTCTACGACC+TGG - chr2.3:70131426-70131445 MS.gene047374:intron 55.0%
ATTCCCCCAGGTCGTAGAAC+TGG + chr2.3:70131435-70131454 None:intergenic 55.0%
CACAAGTTCAACCCTTCCGC+GGG + chr2.3:70131348-70131367 None:intergenic 55.0%
CCAGATGCTTCGTGAGTGGA+TGG - chr2.3:70130240-70130259 MS.gene047374:CDS 55.0%
CCCATGTCAGCCTTGGCATT+AGG + chr2.3:70132082-70132101 None:intergenic 55.0%
CTAGGAAAGCGGAAGGAGGA+GGG + chr2.3:70131293-70131312 None:intergenic 55.0%
GAACCTAGGAAAGCGGAAGG+AGG + chr2.3:70131297-70131316 None:intergenic 55.0%
GAAGCGCGACATCCACACTA+CGG + chr2.3:70130204-70130223 None:intergenic 55.0%
GCTACTGCAGCTCTGTGAAG+AGG - chr2.3:70130479-70130498 MS.gene047374:CDS 55.0%
TAGCCCCAGTTCTACGACCT+GGG - chr2.3:70131427-70131446 MS.gene047374:intron 55.0%
TAGGAAGGAGAGCCGTAGTG+TGG - chr2.3:70130189-70130208 MS.gene047374:CDS 55.0%
TGTGGATGTCGCGCTTCTTG+AGG - chr2.3:70130207-70130226 MS.gene047374:CDS 55.0%
TTCCCCCAGGTCGTAGAACT+GGG + chr2.3:70131434-70131453 None:intergenic 55.0%
TTGGGACTGTTCAAGACCCG+CGG - chr2.3:70131329-70131348 MS.gene047374:intron 55.0%
! CCATCCACTCACGAAGCATC+TGG + chr2.3:70130243-70130262 None:intergenic 55.0%
! GGCACAGGCTTTAGTGAGGA+TGG - chr2.3:70131228-70131247 MS.gene047374:intron 55.0%
AGCCCCAGTTCTACGACCTG+GGG - chr2.3:70131428-70131447 MS.gene047374:intron 60.0%
CATCCCAGAATGCGAGGGAG+CGG - chr2.3:70131518-70131537 MS.gene047374:intron 60.0%
CCTAGGAAAGCGGAAGGAGG+AGG + chr2.3:70131294-70131313 None:intergenic 60.0%
CCTCCTCCTTCCGCTTTCCT+AGG - chr2.3:70131291-70131310 MS.gene047374:intron 60.0%
CGGCAACTGCAAAGTCTCCG+TGG - chr2.3:70131538-70131557 MS.gene047374:intron 60.0%
CGTAGAACTGGGGCTATGCC+TGG + chr2.3:70131423-70131442 None:intergenic 60.0%
GAAGGAGGAGGGCAGATGCT+TGG + chr2.3:70131282-70131301 None:intergenic 60.0%
GACTGTTCAAGACCCGCGGA+AGG - chr2.3:70131333-70131352 MS.gene047374:intron 60.0%
GTCGGCATCCCAGAATGCGA+GGG - chr2.3:70131513-70131532 MS.gene047374:intron 60.0%
TCCCCCAGGTCGTAGAACTG+GGG + chr2.3:70131433-70131452 None:intergenic 60.0%
TGTCGGCATCCCAGAATGCG+AGG - chr2.3:70131512-70131531 MS.gene047374:intron 60.0%
TTGCCGCTCCCTCGCATTCT+GGG + chr2.3:70131524-70131543 None:intergenic 60.0%
GCCCCAGTTCTACGACCTGG+GGG - chr2.3:70131429-70131448 MS.gene047374:intron 65.0%
GTTGCCGCTCCCTCGCATTC+TGG + chr2.3:70131525-70131544 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 70130082 70132177 70130082 ID=MS.gene047374
chr2.3 mRNA 70130082 70132177 70130082 ID=MS.gene047374.t1;Parent=MS.gene047374
chr2.3 exon 70131956 70132177 70131956 ID=MS.gene047374.t1.exon1;Parent=MS.gene047374.t1
chr2.3 CDS 70131956 70132177 70131956 ID=cds.MS.gene047374.t1;Parent=MS.gene047374.t1
chr2.3 exon 70131669 70131818 70131669 ID=MS.gene047374.t1.exon2;Parent=MS.gene047374.t1
chr2.3 CDS 70131669 70131818 70131669 ID=cds.MS.gene047374.t1;Parent=MS.gene047374.t1
chr2.3 exon 70130689 70131199 70130689 ID=MS.gene047374.t1.exon3;Parent=MS.gene047374.t1
chr2.3 CDS 70130689 70131199 70130689 ID=cds.MS.gene047374.t1;Parent=MS.gene047374.t1
chr2.3 exon 70130082 70130596 70130082 ID=MS.gene047374.t1.exon4;Parent=MS.gene047374.t1
chr2.3 CDS 70130082 70130596 70130082 ID=cds.MS.gene047374.t1;Parent=MS.gene047374.t1
Gene Sequence

>MS.gene047374

ATGATTTCCAAGGGTAGCTGTAAGAAAGCATCACACAGGCTTATCAAGGACAAGGCAAAGAACCATGTAGATGATCTGCATGTGATGTTCCTTGATCTGCAGTTTGCTAGGAAGGAGAGCCGTAGTGTGGATGTCGCGCTTCTTGAGGAGCAAGTTCACCAGATGCTTCGTGAGTGGATGGCCGAGCTCAATGAGCCTTCTCCTGCTTCTTCTTTGCAGCAGGTAAGCTCAGAATAGTTCTCCTTAACTTTAATATGCAGATTTAATTGTGGATTTGTGATGGACACATGTAAACTTGAATGGCAATATTTGTGGCAAAAACAATGATTTGAGTTTGCCATGTTTATGGGTGATGATAGGGTGGCAGTCTTCGATCATTCTCGACCGATATATGTAGGCTACTGCAGCTCTGTGAAGAGGAAGATGATGCCACTAGTCCATTAGCTGTGCCTAAGCATGAACCTAATGATCAGAATATGCAGACTGAAGCTAAGATGACGTTTCAAGAAGTCTGTTCCTTTCTCCTCTTCAGCGATTATTCATACATCTTTGTGATTTGATCATAACTGGCATAGTTTTTATTTTTTCTGTAGTATTGGCAGTCTAAAATATTGATTTGAAGCTTCTTTTTCTTTATTGCATTATTTTAATTCATATACTAGAGTAAGGGATGCTTTGGAATGTATGATATCTTCTTTTAATGCAAACAGAAAAATATTATAAAGTGTTGTATTGTTTTTTATATGTGGTAGCCTTTTCTCTAAAAAATAAAATTTAATCTTTTATTTTCAATTGACAGAAATGGAGAGCAAGGATTTGATGGTTCAGTCTTTCCTTAGTTAATACCAATTTTCTCACCAACAACTCAAAGCCCAATTTGTTCTTATGCATCATAGTTTTAATCATTATTTCAATGCTTGTCAATATTTTGATCTCACATATGATTGTAACAAGTTTCTTGGTTTAAATTTAAACCAGAGCCAACATCAGCATGATTCCCTATTAATGGATGAGTACATACACTCCATCTTAGGAGCTCCCAGTAAATCAACTAACAACTCGGATAGGCGGCCTAATTTTGAATCAAAATGTCGTCAATTTGATTTAAATGAAGATTTTAATCACAACTGTTATACTGCTTTTAGAGGCACAGGCTTTAGTGAGGATGGTGTTGTTCCTTATATACCTACCTATCTACCAAGCATCTGCCCTCCTCCTTCCGCTTTCCTAGGTTCAAAATGTGCACTTTGGGACTGTTCAAGACCCGCGGAAGGGTTGAACTTGTGTCAAGACTATTGCAGTAGTTTTCATGCTGCTCTTGCTTTAAATGAAGGACAACCAGGCATAGCCCCAGTTCTACGACCTGGGGGAATTGAAGTTAAGGATAATTTACTGTTGGCTGCTCTTAGTGCAAAGACAGAAGGAAAAGATGTCGGCATCCCAGAATGCGAGGGAGCGGCAACTGCAAAGTCTCCGTGGAATGCACCTGGTATTAGAATGTAGAATGCTGAGTTTACCTATTAAACTATTTATGTGTTTATTGAGTATAAAAGTTGAGAAGAGTGTTCTTTTTTGTTGCAGAGCTCTTTGATCTTTTTGTTCTTGAGGGGGAGACTATTAGGGAGTGGCTTTTCTTTGATAAGTCTCGAAGAGCATTTGAGAGTGGAAACAGAAAGCAAAGGTCGTTGCCTGATTATAATGGGCGGGGATGGCATGAATCCAGAAAGCAAGTGATGAAAGAGTTTGGAGGGCTGAAGAGATCCTACTACATGGATCCACAACCACTAAACCATTTTGAGTGGCATCTTTATGAGTATGAAATTAGCAAGTGTGATGCATGTGCGTTATATCGGTTGGAACTAAAGCTCGTTAATGGGAAGAAGAGCTCCAGGACAAAGATAACAAATGATTCAGTTACTGATCTTCAGAAGCGGATAAGAAGACTCTCTGCCGAGTTTCCCTATGATAACAGACATTCTGCTAAGGGCAGAGCCAAACCTAATGCCAAGGCTGACATGGGTGGTGTTTATACAGCTTCAAACAATGCACCATCACCATATGACTATCTTGTTGAGAGCACAATCGCTATTATGTGA
Protein sequence

>MS.gene047374.t1

MISKGSCKKASHRLIKDKAKNHVDDLHVMFLDLQFARKESRSVDVALLEEQVHQMLREWMAELNEPSPASSLQQGGSLRSFSTDICRLLQLCEEEDDATSPLAVPKHEPNDQNMQTEAKMTFQESQHQHDSLLMDEYIHSILGAPSKSTNNSDRRPNFESKCRQFDLNEDFNHNCYTAFRGTGFSEDGVVPYIPTYLPSICPPPSAFLGSKCALWDCSRPAEGLNLCQDYCSSFHAALALNEGQPGIAPVLRPGGIEVKDNLLLAALSAKTEGKDVGIPECEGAATAKSPWNAPELFDLFVLEGETIREWLFFDKSRRAFESGNRKQRSLPDYNGRGWHESRKQVMKEFGGLKRSYYMDPQPLNHFEWHLYEYEISKCDACALYRLELKLVNGKKSSRTKITNDSVTDLQKRIRRLSAEFPYDNRHSAKGRAKPNAKADMGGVYTASNNAPSPYDYLVESTIAIM