AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047419.t1 MTR_7g110060 83.051 59 5 1 15 73 238 291 2.81e-24 97.8
MS.gene047419.t1 MTR_7g110060 83.051 59 5 1 15 73 238 291 3.74e-24 98.2
MS.gene047419.t1 MTR_1g086370 81.356 59 6 1 15 73 119 172 1.38e-23 94.7
MS.gene047419.t1 MTR_1g084760 81.356 59 6 1 15 73 238 291 2.97e-23 95.5
MS.gene047419.t1 MTR_1g086370 81.356 59 6 1 15 73 205 258 3.49e-23 95.1
MS.gene047419.t1 MTR_1g086370 81.356 59 6 1 15 73 238 291 4.13e-23 95.1
MS.gene047419.t1 MTR_7g076030 77.966 59 8 1 15 73 304 357 8.34e-23 94.7
MS.gene047419.t1 MTR_7g076030 77.966 59 8 1 15 73 197 250 8.47e-23 94.0
MS.gene047419.t1 MTR_2g005620 79.661 59 7 1 15 73 253 306 9.57e-23 94.4
MS.gene047419.t1 MTR_8g432510 77.966 59 8 1 15 73 302 355 3.52e-22 92.8
MS.gene047419.t1 MTR_2g083940 76.271 59 9 1 15 73 197 250 3.28e-21 89.7
MS.gene047419.t1 MTR_2g083940 76.271 59 9 1 15 73 251 304 3.74e-21 89.7
MS.gene047419.t1 MTR_2g016490 72.881 59 11 1 15 73 203 256 2.22e-20 87.4
MS.gene047419.t1 MTR_5g078250 40.476 126 58 2 66 174 332 457 3.93e-17 78.6
MS.gene047419.t1 MTR_8g062030 39.450 109 51 2 66 159 333 441 1.65e-16 77.0
MS.gene047419.t1 MTR_0042s0170 34.783 138 62 3 66 184 164 292 3.82e-12 64.3
MS.gene047419.t1 MTR_7g114300 40.541 74 39 1 6 79 261 329 1.74e-11 62.4
MS.gene047419.t1 MTR_7g114300 40.541 74 39 1 6 79 261 329 1.89e-11 62.0
MS.gene047419.t1 MTR_7g114300 40.541 74 39 1 6 79 261 329 2.00e-11 62.0
MS.gene047419.t1 MTR_7g114300 43.939 66 32 1 14 79 269 329 3.60e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 119 172 6.02e-23 93.2
MS.gene047419.t1 AT3G05840 79.661 59 7 1 15 73 236 289 6.07e-23 94.7
MS.gene047419.t1 AT3G05840 79.661 59 7 1 15 73 236 289 6.07e-23 94.7
MS.gene047419.t1 AT5G26751 79.661 59 7 1 15 73 232 285 6.99e-23 94.7
MS.gene047419.t1 AT1G57870 79.661 59 7 1 15 73 245 298 7.56e-23 94.7
MS.gene047419.t1 AT1G57870 79.661 59 7 1 15 73 245 298 7.56e-23 94.7
MS.gene047419.t1 AT1G57870 79.661 59 7 1 15 73 245 298 7.56e-23 94.7
MS.gene047419.t1 AT1G57870 79.661 59 7 1 15 73 245 298 1.04e-22 94.4
MS.gene047419.t1 AT5G14640 79.661 59 7 1 15 73 237 290 1.45e-22 93.6
MS.gene047419.t1 AT5G14640 79.661 59 7 1 15 73 237 290 1.45e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT1G09840 77.966 59 8 1 15 73 246 299 2.02e-22 93.6
MS.gene047419.t1 AT4G00720 74.576 59 10 1 15 73 301 354 1.39e-21 91.3
MS.gene047419.t1 AT4G18710 74.576 59 10 1 15 73 197 250 2.36e-21 88.6
MS.gene047419.t1 AT1G06390 76.271 59 9 1 15 73 233 286 2.79e-21 90.1
MS.gene047419.t1 AT1G06390 76.271 59 9 1 15 73 233 286 2.79e-21 90.1
MS.gene047419.t1 AT2G30980 76.271 59 9 1 15 73 235 288 5.27e-21 89.4
MS.gene047419.t1 AT4G18710 74.576 59 10 1 15 73 203 256 7.27e-21 89.0
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 265 318 1.06e-20 88.2
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 272 325 1.32e-20 87.8
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 265 318 1.38e-20 88.2
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 265 318 2.26e-20 87.8
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 265 318 2.45e-20 87.8
MS.gene047419.t1 AT3G61160 71.186 59 12 1 15 73 272 325 2.55e-20 87.8
MS.gene047419.t1 AT2G46070 32.394 142 73 4 14 148 237 362 4.24e-13 67.0
MS.gene047419.t1 AT2G46070 32.394 142 73 4 14 148 203 328 4.82e-13 66.6
MS.gene047419.t1 AT2G46070 55.556 54 19 1 14 67 203 251 1.43e-12 65.1
MS.gene047419.t1 AT1G01560 45.588 68 32 1 14 81 202 264 1.07e-11 62.4
MS.gene047419.t1 AT1G01560 45.588 68 32 1 14 81 202 264 1.07e-11 62.4

Find 33 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCTTACATTTGTTTCT+AGG 0.116231 2.4:-74190534 MS.gene047419:CDS
TTTAAAGTTCAGGCAGTTAT+TGG 0.206518 2.4:-74188880 MS.gene047419:CDS
TGCTCAGTTGGTTGCGTACT+TGG 0.314908 2.4:-74190451 MS.gene047419:CDS
AGATAAACAATGTGCTCATT+TGG 0.319034 2.4:-74189062 MS.gene047419:CDS
TGAGATTTACTCTCCTTCTA+AGG 0.373862 2.4:-74188773 MS.gene047419:intron
ATTGATACTGAGAAGATTGT+TGG 0.390696 2.4:-74188637 MS.gene047419:CDS
AAACAAATGTAAGATATGTT+TGG 0.394876 2.4:+74190539 None:intergenic
CTTTGTATGATGGTTCCTAT+AGG 0.404739 2.4:-74188849 MS.gene047419:CDS
CTTTGGAAGTTCTAATGTCT+TGG 0.417717 2.4:-74190662 None:intergenic
ATGGTTCCTATAGGGTGATA+AGG 0.433018 2.4:-74188840 MS.gene047419:CDS
GGACCCCATGCTTTGTATGA+TGG 0.435370 2.4:-74188859 MS.gene047419:CDS
ACAAACCCTTATCACCCTAT+AGG 0.444255 2.4:+74188834 None:intergenic
ATACACAGAAGCTAGTTAAC+TGG 0.448429 2.4:+74190413 None:intergenic
TGGTTCCTATAGGGTGATAA+GGG 0.460025 2.4:-74188839 MS.gene047419:CDS
AGAGCTCCTGAGCTTATATT+CGG 0.474562 2.4:-74190505 MS.gene047419:CDS
TCATTTGGTTGATGTAGCTA+AGG 0.482770 2.4:-74189047 MS.gene047419:intron
TTTGTATGATGGTTCCTATA+GGG 0.492577 2.4:-74188848 MS.gene047419:CDS
GCCATTGACATCTGCTCAGT+TGG 0.493750 2.4:-74190463 MS.gene047419:CDS
CAAACTTGTGGAAATTATCA+AGG 0.511188 2.4:-74190295 MS.gene047419:intron
AGGAACCATCATACAAAGCA+TGG 0.519567 2.4:+74188854 None:intergenic
CATCAGTTACATCAGATGCT+TGG 0.530953 2.4:+74189128 None:intergenic
TACTGAGAAGATTGTTGGCT+CGG 0.541038 2.4:-74188632 MS.gene047419:CDS
ACCAACTGAGCAGATGTCAA+TGG 0.561044 2.4:+74190462 None:intergenic
GGAACCATCATACAAAGCAT+GGG 0.561104 2.4:+74188855 None:intergenic
GACTGTTTCACTTGATGATG+AGG 0.575402 2.4:-74188662 MS.gene047419:CDS
CCACTGTTTCCTAGTGAGAG+TGG 0.575434 2.4:-74190324 MS.gene047419:CDS
TTGTCTACTCCACTCTCACT+AGG 0.578433 2.4:+74190315 None:intergenic
GTTGCACCGAATATAAGCTC+AGG 0.582148 2.4:+74190499 None:intergenic
GGTTGATGCTTCGCAGACTG+AGG 0.585844 2.4:-74188611 MS.gene047419:CDS
TGAGCAGATGTCAATGGCTA+TGG 0.611943 2.4:+74190468 None:intergenic
GAGTGGAGTAGACAAACTTG+TGG 0.646343 2.4:-74190307 MS.gene047419:CDS
CCACTCTCACTAGGAAACAG+TGG 0.673051 2.4:+74190324 None:intergenic
GAACCATCATACAAAGCATG+GGG 0.696724 2.4:+74188856 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAACTCTGTATAATT+AGG + chr2.4:74189171-74189190 None:intergenic 15.0%
!! ATAAGAAAAATATTACATGA+TGG - chr2.4:74189636-74189655 MS.gene047419:intron 15.0%
!! TAAGAAAAATATTACATGAT+GGG - chr2.4:74189637-74189656 MS.gene047419:intron 15.0%
!! TTTGAAAAAAAAAACTTTGT+TGG + chr2.4:74189765-74189784 None:intergenic 15.0%
!!! ATTTTCTTTGAAAAAGAATA+TGG - chr2.4:74190282-74190301 MS.gene047419:intron 15.0%
!!! TTTTCTTTGAAAAAGAATAT+GGG - chr2.4:74190283-74190302 MS.gene047419:intron 15.0%
!! AAACAAATGTAAGATATGTT+TGG + chr2.4:74188709-74188728 None:intergenic 20.0%
!! AAATTCATAAACTCAAATCA+AGG + chr2.4:74190026-74190045 None:intergenic 20.0%
!! AAGAAAAATATTACATGATG+GGG - chr2.4:74189638-74189657 MS.gene047419:intron 20.0%
!! AATGAGATGGATAAATTAAA+GGG - chr2.4:74190329-74190348 MS.gene047419:intron 20.0%
!! AGTTCAAATTCAATCAAATT+TGG + chr2.4:74189835-74189854 None:intergenic 20.0%
!! TAATGAGATGGATAAATTAA+AGG - chr2.4:74190328-74190347 MS.gene047419:intron 20.0%
!! TTGAACTTGAATAATTAGTT+TGG - chr2.4:74189848-74189867 MS.gene047419:intron 20.0%
!! TTTGAATGAATTTAATGAGT+AGG - chr2.4:74189457-74189476 MS.gene047419:intron 20.0%
!!! ATAGCTATCTGAATTTTATA+AGG - chr2.4:74188647-74188666 MS.gene047419:CDS 20.0%
!!! GAATTTTACTATATGTTACA+TGG - chr2.4:74190040-74190059 MS.gene047419:intron 20.0%
!!! GCACATGTTTATATTTTATT+TGG - chr2.4:74189594-74189613 MS.gene047419:intron 20.0%
!!! TAAATGCTATATGATTTTAC+AGG - chr2.4:74188672-74188691 MS.gene047419:intron 20.0%
!!! TATATGATTTTACAGGTTAA+AGG - chr2.4:74188679-74188698 MS.gene047419:intron 20.0%
!!! TATTTGTTTTGAATTGTTCA+AGG - chr2.4:74190520-74190539 MS.gene047419:CDS 20.0%
!!! TTTAATTTATCCATCTCATT+AGG + chr2.4:74190329-74190348 None:intergenic 20.0%
! AAACAAATAACCAACAAATG+AGG + chr2.4:74190509-74190528 None:intergenic 25.0%
! AAACGAAATGAAAAGATGAA+AGG + chr2.4:74189054-74189073 None:intergenic 25.0%
! AAATCAACTTTATGTCAGTT+GGG + chr2.4:74189487-74189506 None:intergenic 25.0%
! AACGCCTAAATGATAAATTT+AGG + chr2.4:74190563-74190582 None:intergenic 25.0%
! ATAATTAGAGTTGAGTTTAC+AGG - chr2.4:74189999-74190018 MS.gene047419:intron 25.0%
! ATATCATAAGTTATGTACCA+TGG + chr2.4:74189924-74189943 None:intergenic 25.0%
! ATCACAATATAGTTAACACA+TGG + chr2.4:74189981-74190000 None:intergenic 25.0%
! ATGAGATGGATAAATTAAAG+GGG - chr2.4:74190330-74190349 MS.gene047419:intron 25.0%
! TGGACCTAAATTTATCATTT+AGG - chr2.4:74190556-74190575 MS.gene047419:intron 25.0%
! TGGTACATAACTTATGATAT+TGG - chr2.4:74189924-74189943 MS.gene047419:intron 25.0%
!! AATTTTTACACCTCATTTGT+TGG - chr2.4:74190496-74190515 MS.gene047419:CDS 25.0%
!! CATATCTTACATTTGTTTCT+AGG - chr2.4:74188711-74188730 MS.gene047419:intron 25.0%
!! TGTAGAATGTAGAATTTGTT+TGG - chr2.4:74190246-74190265 MS.gene047419:intron 25.0%
AAAATGAAAATAGGGAACCA+TGG - chr2.4:74189904-74189923 MS.gene047419:intron 30.0%
AAACAAACGAAAGGATTTCA+AGG + chr2.4:74188623-74188642 None:intergenic 30.0%
ACAATATAGTTAACACATGG+TGG + chr2.4:74189978-74189997 None:intergenic 30.0%
ACTTAAGAGCTTAAGGATAA+AGG - chr2.4:74189716-74189735 MS.gene047419:intron 30.0%
AGATAAACAATGTGCTCATT+TGG - chr2.4:74190183-74190202 MS.gene047419:intron 30.0%
AGGATGAAATCCAACATTAT+CGG + chr2.4:74190309-74190328 None:intergenic 30.0%
ATATGTTCAAGATAGTAGCA+AGG + chr2.4:74189018-74189037 None:intergenic 30.0%
ATTGATACTGAGAAGATTGT+TGG - chr2.4:74190608-74190627 MS.gene047419:intron 30.0%
CAAACTTGTGGAAATTATCA+AGG - chr2.4:74188950-74188969 MS.gene047419:intron 30.0%
CAAATCAACTTTATGTCAGT+TGG + chr2.4:74189488-74189507 None:intergenic 30.0%
CAAATCTCATTCATGACATA+AGG - chr2.4:74189200-74189219 MS.gene047419:intron 30.0%
CAATATAGTTAACACATGGT+GGG + chr2.4:74189977-74189996 None:intergenic 30.0%
GGTGTAAAAATTACCTTAGA+AGG + chr2.4:74190488-74190507 None:intergenic 30.0%
TTAAGCTCTTAAGTCTTCAT+CGG + chr2.4:74189710-74189729 None:intergenic 30.0%
TTCAATCAAATTTGGCATGT+AGG + chr2.4:74189827-74189846 None:intergenic 30.0%
TTCATTCAAACTGAATGTGA+AGG + chr2.4:74189447-74189466 None:intergenic 30.0%
TTTAACCTTAAACGGTCTAA+AGG + chr2.4:74190095-74190114 None:intergenic 30.0%
TTTGTATGATGGTTCCTATA+GGG - chr2.4:74190397-74190416 MS.gene047419:intron 30.0%
! CAACTGACATAAAGTTGATT+TGG - chr2.4:74189486-74189505 MS.gene047419:intron 30.0%
! TGATTTCTTCATCTTTTGAG+TGG - chr2.4:74189411-74189430 MS.gene047419:intron 30.0%
! TTTAAAGTTCAGGCAGTTAT+TGG - chr2.4:74190365-74190384 MS.gene047419:intron 30.0%
! TTTTCAAAACTCATTCCATG+AGG - chr2.4:74189777-74189796 MS.gene047419:intron 30.0%
!! CATGAATGAGATTTGATTTG+AGG + chr2.4:74189195-74189214 None:intergenic 30.0%
!! TTCAAGGCTATGTTTTTCAA+TGG - chr2.4:74190536-74190555 MS.gene047419:CDS 30.0%
!!! GAAGATGCTTTTTAAAGTTC+AGG - chr2.4:74190355-74190374 MS.gene047419:intron 30.0%
!!! TGATGTAGCTAAGGTTTTTT+CGG - chr2.4:74190207-74190226 MS.gene047419:intron 30.0%
AAGTAGGCAATCTTCAAAGT+AGG - chr2.4:74189674-74189693 MS.gene047419:intron 35.0%
AAGTGAACCACATGAAACTT+CGG + chr2.4:74189102-74189121 None:intergenic 35.0%
ACTTGATTTCTTCCCTTGTT+GGG + chr2.4:74189140-74189159 None:intergenic 35.0%
AGTTTGGTCAAGTTACGAAT+TGG - chr2.4:74189864-74189883 MS.gene047419:intron 35.0%
ATACACAGAAGCTAGTTAAC+TGG + chr2.4:74188835-74188854 None:intergenic 35.0%
ATACACCTTTAGACCGTTTA+AGG - chr2.4:74190087-74190106 MS.gene047419:intron 35.0%
CTTTGTATGATGGTTCCTAT+AGG - chr2.4:74190396-74190415 MS.gene047419:intron 35.0%
GATGAAGACTTAAGAGCTTA+AGG - chr2.4:74189709-74189728 MS.gene047419:intron 35.0%
GCTATTGCAAAACAAACGAA+AGG + chr2.4:74188632-74188651 None:intergenic 35.0%
GCTTGGAGTTTAACCTTAAA+CGG + chr2.4:74190103-74190122 None:intergenic 35.0%
GTTTATGCAATGAATGAGCA+AGG + chr2.4:74189269-74189288 None:intergenic 35.0%
TAAGCTCTTAAGTCTTCATC+GGG + chr2.4:74189709-74189728 None:intergenic 35.0%
TGAGATTTACTCTCCTTCTA+AGG - chr2.4:74190472-74190491 MS.gene047419:CDS 35.0%
TGGATTTCATCCTAATGAGA+TGG - chr2.4:74190316-74190335 MS.gene047419:CDS 35.0%
! TCATTTGGTTGATGTAGCTA+AGG - chr2.4:74190198-74190217 MS.gene047419:intron 35.0%
! TTTTTGTTACCTAGACCTCA+TGG + chr2.4:74189795-74189814 None:intergenic 35.0%
AAACTCATTCCATGAGGTCT+AGG - chr2.4:74189783-74189802 MS.gene047419:intron 40.0%
ACAAACCCTTATCACCCTAT+AGG + chr2.4:74190414-74190433 None:intergenic 40.0%
AGAATATGGGCCGATAATGT+TGG - chr2.4:74190296-74190315 MS.gene047419:CDS 40.0%
AGAGCTCCTGAGCTTATATT+CGG - chr2.4:74188740-74188759 MS.gene047419:intron 40.0%
AGGAACCATCATACAAAGCA+TGG + chr2.4:74190394-74190413 None:intergenic 40.0%
ATGGTTCCTATAGGGTGATA+AGG - chr2.4:74190405-74190424 MS.gene047419:intron 40.0%
CACTTGATTTCTTCCCTTGT+TGG + chr2.4:74189141-74189160 None:intergenic 40.0%
CATCAGTTACATCAGATGCT+TGG + chr2.4:74190120-74190139 None:intergenic 40.0%
CTTGATTTCTTCCCTTGTTG+GGG + chr2.4:74189139-74189158 None:intergenic 40.0%
GAACCATCATACAAAGCATG+GGG + chr2.4:74190392-74190411 None:intergenic 40.0%
GGAACCATCATACAAAGCAT+GGG + chr2.4:74190393-74190412 None:intergenic 40.0%
GGGATCATAAATGCTGAAGT+AGG - chr2.4:74189658-74189677 MS.gene047419:intron 40.0%
GTTCATTCCGAAGTTTCATG+TGG - chr2.4:74189092-74189111 MS.gene047419:CDS 40.0%
TGGTTCCTATAGGGTGATAA+GGG - chr2.4:74190406-74190425 MS.gene047419:intron 40.0%
! AACTAGCTTCTGTGTATGCT+TGG - chr2.4:74188838-74188857 MS.gene047419:CDS 40.0%
! TACTGAGAAGATTGTTGGCT+CGG - chr2.4:74190613-74190632 MS.gene047419:intron 40.0%
!! GACTGTTTCACTTGATGATG+AGG - chr2.4:74190583-74190602 MS.gene047419:intron 40.0%
!! GTACTTGGTGAACTTTTGCT+TGG - chr2.4:74188809-74188828 MS.gene047419:CDS 40.0%
!!! GTTGCTTTTGACACTGACAT+GGG - chr2.4:74190151-74190170 MS.gene047419:intron 40.0%
!!! TGTTGCTTTTGACACTGACA+TGG - chr2.4:74190150-74190169 MS.gene047419:intron 40.0%
ACCAACTGAGCAGATGTCAA+TGG + chr2.4:74188786-74188805 None:intergenic 45.0%
GAGTGGAGTAGACAAACTTG+TGG - chr2.4:74188938-74188957 MS.gene047419:intron 45.0%
GTTGCACCGAATATAAGCTC+AGG + chr2.4:74188749-74188768 None:intergenic 45.0%
GTTTCATGTGGTTCACTTGC+AGG - chr2.4:74189104-74189123 MS.gene047419:CDS 45.0%
TGAGCAGATGTCAATGGCTA+TGG + chr2.4:74188780-74188799 None:intergenic 45.0%
TTGTCTACTCCACTCTCACT+AGG + chr2.4:74188933-74188952 None:intergenic 45.0%
! GTGGTTCACTTGCAGGTTTT+AGG - chr2.4:74189111-74189130 MS.gene047419:CDS 45.0%
!!! AGGTTTTAGGTACCCCAACA+AGG - chr2.4:74189124-74189143 MS.gene047419:CDS 45.0%
!!! GGTTTTAGGTACCCCAACAA+GGG - chr2.4:74189125-74189144 MS.gene047419:CDS 45.0%
!! AAAAAAATAAAAATGAAAAT+AGG - chr2.4:74189895-74189914 MS.gene047419:intron 5.0%
!! AAAAAATAAAAATGAAAATA+GGG - chr2.4:74189896-74189915 MS.gene047419:intron 5.0%
CCACTCTCACTAGGAAACAG+TGG + chr2.4:74188924-74188943 None:intergenic 50.0%
CCACTGTTTCCTAGTGAGAG+TGG - chr2.4:74188921-74188940 MS.gene047419:intron 50.0%
CTTAAGTCTTCATCGGGTGG+AGG + chr2.4:74189703-74189722 None:intergenic 50.0%
GCCATTGACATCTGCTCAGT+TGG - chr2.4:74188782-74188801 MS.gene047419:CDS 50.0%
GCTCTTAAGTCTTCATCGGG+TGG + chr2.4:74189706-74189725 None:intergenic 50.0%
GGACCCCATGCTTTGTATGA+TGG - chr2.4:74190386-74190405 MS.gene047419:intron 50.0%
TGCTCAGTTGGTTGCGTACT+TGG - chr2.4:74188794-74188813 MS.gene047419:CDS 50.0%
! GGTTGATGCTTCGCAGACTG+AGG - chr2.4:74190634-74190653 MS.gene047419:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 74188597 74190670 74188597 ID=MS.gene047419
chr2.4 mRNA 74188597 74190670 74188597 ID=MS.gene047419.t1;Parent=MS.gene047419
chr2.4 exon 74190663 74190670 74190663 ID=MS.gene047419.t1.exon1;Parent=MS.gene047419.t1
chr2.4 CDS 74190663 74190670 74190663 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
chr2.4 exon 74190417 74190573 74190417 ID=MS.gene047419.t1.exon2;Parent=MS.gene047419.t1
chr2.4 CDS 74190417 74190573 74190417 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
chr2.4 exon 74190296 74190346 74190296 ID=MS.gene047419.t1.exon3;Parent=MS.gene047419.t1
chr2.4 CDS 74190296 74190346 74190296 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
chr2.4 exon 74189048 74189158 74189048 ID=MS.gene047419.t1.exon4;Parent=MS.gene047419.t1
chr2.4 CDS 74189048 74189158 74189048 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
chr2.4 exon 74188774 74188917 74188774 ID=MS.gene047419.t1.exon5;Parent=MS.gene047419.t1
chr2.4 CDS 74188774 74188917 74188774 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
chr2.4 exon 74188597 74188689 74188597 ID=MS.gene047419.t1.exon6;Parent=MS.gene047419.t1
chr2.4 CDS 74188597 74188689 74188597 ID=cds.MS.gene047419.t1;Parent=MS.gene047419.t1
Gene Sequence

>MS.gene047419

ATGTCTTGGTTCGTTTATCTTAACCTTGAAATCCTTTCGTTTGTTTTGCAATAGCTATCTGAATTTTATAAGGACTAAATGCTATATGATTTTACAGGTTAAAGGTGAACCAAACATATCTTACATTTGTTTCTAGGTACTATAGAGCTCCTGAGCTTATATTCGGTGCAACTGAGTACACCATAGCCATTGACATCTGCTCAGTTGGTTGCGTACTTGGTGAACTTTTGCTTGGCCAGTTAACTAGCTTCTGTGTATGCTTGGACAAGTTACATTTAGAATTCATGTATGTCAAAACTAATGATGAATTTGTGTTCTTATCAGCCACTGTTTCCTAGTGAGAGTGGAGTAGACAAACTTGTGGAAATTATCAAGGTAAGATATGTTTTAAACAATAGTATAGCAATAGTGATATTTACCTTGCTACTATCTTGAACATATCAGTTTTAAGTGACCTTTCATCTTTTCATTTCGTTTTGTGCTATTTTTAGATTAGTTCATTCCGAAGTTTCATGTGGTTCACTTGCAGGTTTTAGGTACCCCAACAAGGGAAGAAATCAAGTGCATGAATCCTAATTATACAGAGTTTAAATTTCCTCAAATCAAATCTCATTCATGACATAAGGTATGTAATGTTAGTGTGTCAAACAAAACAGTATAACATTGATTCCTTGCTCATTCATTGCATAAACTATGTACATGTATTTATATATCAGTTATGTCATGTTCTTATAAAAACTGTCATGTTCTTATGTCATGTTCTTATATAAAATGCTGATAATTTAAATGTAAATTTTGCTTCTGCTGCATAATTTGATTTCTTCATCTTTTGAGTGGTAAGTTAGCACCTTCACATTCAGTTTGAATGAATTTAATGAGTAGGATCACCCAACTGACATAAAGTTGATTTGGTGTTACGTTTTGTGTTTCTTAAGATTTTGATAGTGAAATTGCATAATATGAAATGCAAGTTCACTAGCTTATTTTAAATTTTGCTGCACATGTTTATATTTTATTTGGAATTTAAGAGTCAATTCTCATAAGAAAAATATTACATGATGGGGATCATAAATGCTGAAGTAGGCAATCTTCAAAGTAGGCAGCCTCCACCCGATGAAGACTTAAGAGCTTAAGGATAAAGGTTAGACGTCTCACTAACAGTGTACCAACAAAGTTTTTTTTTTCAAAACTCATTCCATGAGGTCTAGGTAACAAAAATAAGCTAAGCCTACATGCCAAATTTGATTGAATTTGAACTTGAATAATTAGTTTGGTCAAGTTACGAATTGGAATCTAGAAAAAAAATAAAAATGAAAATAGGGAACCATGGTACATAACTTATGATATTGGTTTAATGTGAGAAAAGAGAACTATTTTCCCACCATGTGTTAACTATATTGTGATAATTAGAGTTGAGTTTACAGGTCCTTGATTTGAGTTTATGAATTTTACTATATGTTACATGGTGACACTACACATCTATTTTGACAATACACCTTTAGACCGTTTAAGGTTAAACTCCAAGCATCTGATGTAACTGATGATGCAGTGTTTGTTGCTTTTGACACTGACATGGGTTTTCTTCTAGATAAACAATGTGCTCATTTGGTTGATGTAGCTAAGGTTTTTTCGGTTTATATTTTATTTTTTGTAGAATGTAGAATTTGTTTGGAGTGTAATACTCAATTTTCTTTGAAAAAGAATATGGGCCGATAATGTTGGATTTCATCCTAATGAGATGGATAAATTAAAGGGGAAGAAGATGCTTTTTAAAGTTCAGGCAGTTATTGGACCCCATGCTTTGTATGATGGTTCCTATAGGGTGATAAGGGTTTGTATTGATGAAGCTATTATTGAATTGTTTCTCACTGATGATGAGATTTACTCTCCTTCTAAGGTAATTTTTACACCTCATTTGTTGGTTATTTGTTTTGAATTGTTCAAGGCTATGTTTTTCAATGGACCTAAATTTATCATTTAGGCGTTGACTGTTTCACTTGATGATGAGGTTATTGATACTGAGAAGATTGTTGGCTCGGTTGATGCTTCGCAGACTGAGGATTTTGTCTCTTAA
Protein sequence

>MS.gene047419.t1

MSWLKVNQTYLTFVSRYYRAPELIFGATEYTIAIDICSVGCVLGELLLGQLTSFCPLFPSESGVDKLVEIIKVKLQASDVTDDAVFVAFDTDMGFLLDKQCAHLVDVAKGKKMLFKVQAVIGPHALYDGSYRVIRVCIDEAIIELFLTDDEIYSPSKALTVSLDDEVIDTEKIVGSVDASQTEDFVS