AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047476.t1 MTR_2g024430 87.104 442 53 3 4 441 74 515 0.0 788
MS.gene047476.t1 MTR_5g040880 56.798 456 179 8 1 440 61 514 2.16e-162 468
MS.gene047476.t1 MTR_5g082140 52.570 428 153 6 14 435 75 458 1.18e-140 410
MS.gene047476.t1 MTR_5g040740 50.113 443 115 8 3 440 64 405 7.41e-128 376
MS.gene047476.t1 MTR_5g061220 47.490 478 191 13 1 440 71 526 2.37e-124 371
MS.gene047476.t1 MTR_3g073420 41.365 498 227 12 1 440 67 557 2.66e-103 319
MS.gene047476.t1 MTR_2g006270 42.506 447 186 11 1 439 67 450 3.74e-97 299
MS.gene047476.t1 MTR_1g058210 41.611 447 179 14 1 433 64 442 5.15e-94 291
MS.gene047476.t1 MTR_2g006380 38.117 446 200 15 1 439 74 450 7.73e-80 254
MS.gene047476.t1 MTR_2g094570 39.773 352 141 10 1 291 58 399 1.18e-68 230
MS.gene047476.t1 MTR_2g094570 39.773 352 141 10 1 291 58 399 1.67e-68 231
MS.gene047476.t1 MTR_5g060630 36.943 471 204 18 1 393 62 517 2.52e-68 226
MS.gene047476.t1 MTR_5g460740 36.943 471 204 18 1 393 62 517 2.52e-68 226
MS.gene047476.t1 MTR_2g094570 39.773 352 141 10 1 291 114 455 4.18e-68 231
MS.gene047476.t1 MTR_1g064430 39.255 349 147 8 4 290 90 435 2.90e-65 222
MS.gene047476.t1 MTR_1g064430 39.255 349 147 8 4 290 77 422 3.05e-65 222
MS.gene047476.t1 MTR_4g058930 38.286 350 151 9 1 288 57 403 8.11e-65 220
MS.gene047476.t1 MTR_1g094960 34.607 445 211 13 13 393 77 505 1.24e-64 219
MS.gene047476.t1 MTR_5g061890 45.603 307 133 13 4 291 65 356 1.49e-64 213
MS.gene047476.t1 MTR_4g058930 38.286 350 151 9 1 288 57 403 1.65e-64 220
MS.gene047476.t1 MTR_5g060780 46.853 286 119 12 4 270 65 336 3.20e-64 212
MS.gene047476.t1 MTR_5g460920 46.853 286 119 12 4 270 65 336 1.23e-63 211
MS.gene047476.t1 MTR_7g101280 35.655 359 151 10 4 291 81 430 9.54e-56 195
MS.gene047476.t1 MTR_0041s0110 35.655 359 151 10 4 291 81 430 9.54e-56 195
MS.gene047476.t1 MTR_7g101275 38.768 276 134 8 4 255 42 306 2.76e-48 170
MS.gene047476.t1 MTR_2g014770 36.156 307 164 10 15 295 112 412 3.22e-48 174
MS.gene047476.t1 MTR_2g014770 36.156 307 164 10 15 295 112 412 4.96e-48 175
MS.gene047476.t1 MTR_4g088210 31.026 390 219 12 16 375 99 468 9.28e-44 163
MS.gene047476.t1 MTR_4g088210 31.026 390 219 12 16 375 99 468 1.15e-43 163
MS.gene047476.t1 MTR_2g093740 30.412 388 220 12 1 345 107 487 4.08e-43 161
MS.gene047476.t1 MTR_0041s0120 36.842 285 139 7 4 255 42 318 4.30e-43 157
MS.gene047476.t1 MTR_2g093740 30.412 388 220 12 1 345 107 487 7.89e-43 160
MS.gene047476.t1 MTR_2g093740 30.412 388 220 12 1 345 107 487 1.24e-42 160
MS.gene047476.t1 MTR_2g005240 35.855 304 173 10 1 288 75 372 1.59e-42 159
MS.gene047476.t1 MTR_2g093740 32.615 325 179 9 54 345 40 357 1.56e-41 156
MS.gene047476.t1 MTR_8g100050 33.876 307 180 8 1 289 111 412 1.68e-41 157
MS.gene047476.t1 MTR_2g093740 32.615 325 179 9 54 345 40 357 2.18e-41 156
MS.gene047476.t1 MTR_8g100050 33.876 307 180 8 1 289 111 412 2.70e-41 157
MS.gene047476.t1 MTR_4g021580 33.660 306 178 9 1 288 71 369 3.68e-41 155
MS.gene047476.t1 MTR_4g021580 33.660 306 178 9 1 288 71 369 4.62e-41 155
MS.gene047476.t1 MTR_5g060770 37.015 335 147 14 15 334 22 307 3.29e-40 147
MS.gene047476.t1 MTR_8g027440 34.983 303 176 9 1 288 81 377 9.68e-40 151
MS.gene047476.t1 MTR_2g093740 29.426 401 220 13 1 345 107 500 1.02e-39 151
MS.gene047476.t1 MTR_2g093740 29.426 401 220 13 1 345 107 500 1.64e-39 151
MS.gene047476.t1 MTR_2g093740 29.426 401 220 13 1 345 107 500 1.77e-39 151
MS.gene047476.t1 MTR_4g060460 34.058 276 164 6 54 313 168 441 2.30e-39 151
MS.gene047476.t1 MTR_4g060460 36.000 250 141 6 54 288 168 413 3.66e-39 150
MS.gene047476.t1 MTR_2g043250 31.507 292 171 10 1 271 74 357 3.16e-38 148
MS.gene047476.t1 MTR_2g093740 31.361 338 179 10 54 345 40 370 4.04e-38 146
MS.gene047476.t1 MTR_2g018690 32.667 300 169 10 15 293 90 377 4.09e-38 147
MS.gene047476.t1 MTR_2g093740 31.361 338 179 10 54 345 40 370 4.93e-38 147
MS.gene047476.t1 MTR_4g124900 31.849 292 170 10 1 271 74 357 8.53e-38 147
MS.gene047476.t1 MTR_4g124900 31.849 292 170 10 1 271 74 357 8.61e-38 147
MS.gene047476.t1 MTR_7g062540 33.443 305 179 11 1 288 66 363 1.30e-37 145
MS.gene047476.t1 MTR_5g062970 50.000 136 66 1 10 143 94 229 1.51e-37 137
MS.gene047476.t1 MTR_8g079492 32.787 305 162 12 15 293 7 294 2.59e-37 145
MS.gene047476.t1 MTR_8g079492 32.787 305 162 12 15 293 89 376 3.97e-37 145
MS.gene047476.t1 MTR_8g079492 32.787 305 162 12 15 293 89 376 4.05e-37 144
MS.gene047476.t1 MTR_5g076270 32.857 280 163 8 15 275 91 364 4.49e-37 144
MS.gene047476.t1 MTR_3g064050 32.500 280 164 8 15 275 93 366 6.10e-36 141
MS.gene047476.t1 MTR_1g024025 35.971 278 146 13 14 269 109 376 3.60e-35 139
MS.gene047476.t1 MTR_8g101360 31.655 278 163 9 15 272 98 368 3.79e-35 139
MS.gene047476.t1 MTR_5g062960 40.952 210 93 9 168 369 1 187 3.28e-34 127
MS.gene047476.t1 MTR_8g446900 26.950 282 187 6 1 269 13 288 1.25e-27 113
MS.gene047476.t1 MTR_5g460910 30.746 335 146 15 15 334 39 302 1.39e-20 92.8
MS.gene047476.t1 MTR_5g046120 38.835 103 55 3 1 100 26 123 3.09e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047476.t1 AT1G77850 35.381 472 241 15 4 418 67 531 4.46e-73 240
MS.gene047476.t1 AT1G77850 35.593 472 240 16 4 418 67 531 5.32e-73 240
MS.gene047476.t1 AT4G30080 39.674 368 154 11 1 309 65 423 5.14e-67 226
MS.gene047476.t1 AT2G28350 37.571 354 157 10 1 293 56 406 3.28e-62 213
MS.gene047476.t1 AT2G28350 37.571 354 157 10 1 293 56 406 4.93e-62 213
MS.gene047476.t1 AT2G46530 36.364 308 167 12 1 288 74 372 1.40e-43 161
MS.gene047476.t1 AT2G46530 36.364 308 167 12 1 288 95 393 2.87e-43 161
MS.gene047476.t1 AT3G61830 36.014 286 157 11 1 269 27 303 1.60e-41 155
MS.gene047476.t1 AT3G61830 36.014 286 157 11 1 269 78 354 3.42e-41 155
MS.gene047476.t1 AT2G46530 37.638 271 142 11 36 288 24 285 8.81e-41 152
MS.gene047476.t1 AT2G33860 37.931 232 124 7 54 269 4 231 1.29e-40 150
MS.gene047476.t1 AT1G59750 34.737 285 165 8 1 269 74 353 1.91e-40 153
MS.gene047476.t1 AT1G35520 36.452 310 167 12 4 295 82 379 2.06e-40 152
MS.gene047476.t1 AT1G59750 34.737 285 165 8 1 269 74 353 2.14e-40 153
MS.gene047476.t1 AT1G59750 34.737 285 165 8 1 269 74 353 2.14e-40 153
MS.gene047476.t1 AT1G59750 34.737 285 165 8 1 269 74 353 2.22e-40 153
MS.gene047476.t1 AT5G60450 34.853 307 174 11 4 286 119 423 4.79e-40 153
MS.gene047476.t1 AT5G60450 34.853 307 174 11 4 286 95 399 5.42e-40 153
MS.gene047476.t1 AT2G33860 37.931 232 124 7 54 269 161 388 6.42e-40 151
MS.gene047476.t1 AT5G37020 33.571 280 161 8 15 275 89 362 8.46e-40 152
MS.gene047476.t1 AT5G37020 33.571 280 161 8 15 275 89 362 1.07e-39 152
MS.gene047476.t1 AT1G34390 36.964 303 159 14 4 288 82 370 1.92e-39 150
MS.gene047476.t1 AT1G34410 34.839 310 172 11 4 295 82 379 3.22e-39 149
MS.gene047476.t1 AT4G23980 32.867 286 167 9 1 269 65 342 5.22e-39 149
MS.gene047476.t1 AT4G23980 32.867 286 167 9 1 269 65 342 5.46e-39 149
MS.gene047476.t1 AT5G62000 33.904 292 170 8 15 288 127 413 4.73e-38 147
MS.gene047476.t1 AT5G62000 33.904 292 170 8 15 288 127 413 4.91e-38 147
MS.gene047476.t1 AT5G62000 33.904 292 170 8 15 288 127 413 4.91e-38 147
MS.gene047476.t1 AT5G62000 33.904 292 170 8 15 288 127 413 4.91e-38 147
MS.gene047476.t1 AT5G62000 33.904 292 170 8 15 288 127 413 4.91e-38 147
MS.gene047476.t1 AT5G37020 35.685 241 130 8 54 275 33 267 5.36e-38 147
MS.gene047476.t1 AT1G34310 35.314 303 166 12 4 288 82 372 1.73e-37 144
MS.gene047476.t1 AT1G19850 38.793 232 123 7 54 269 149 377 5.65e-37 144
MS.gene047476.t1 AT1G19850 38.793 232 123 7 54 269 160 388 5.82e-37 144
MS.gene047476.t1 AT1G35240 35.216 301 169 11 4 288 82 372 6.40e-37 142
MS.gene047476.t1 AT1G30330 32.857 280 163 8 15 275 90 363 1.41e-36 143
MS.gene047476.t1 AT1G30330 32.857 280 163 8 15 275 92 365 1.58e-36 143
MS.gene047476.t1 AT1G30330 32.857 280 163 8 15 275 92 365 1.58e-36 143
MS.gene047476.t1 AT1G35540 34.164 281 160 10 4 269 82 352 3.39e-36 140
MS.gene047476.t1 AT5G20730 31.900 279 163 8 13 271 88 359 3.50e-35 139
MS.gene047476.t1 AT5G20730 31.900 279 163 8 13 271 88 359 3.79e-35 139
MS.gene047476.t1 AT5G20730 31.900 279 162 9 13 271 88 358 5.91e-34 135
MS.gene047476.t1 AT1G19220 32.374 278 158 9 15 271 89 357 1.87e-33 134
MS.gene047476.t1 AT1G34170 28.643 398 239 17 14 386 90 467 6.79e-31 124
MS.gene047476.t1 AT1G34170 27.763 389 228 17 14 386 90 441 2.05e-25 108
MS.gene047476.t1 AT1G34170 27.632 380 223 16 14 378 90 432 7.72e-25 107
MS.gene047476.t1 AT1G43950 39.333 150 81 5 4 152 82 222 6.84e-20 88.6
MS.gene047476.t1 AT4G01500 30.597 134 90 1 54 187 38 168 2.46e-11 65.1

Find 108 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTTCATTTATGCTATT+TGG 0.105998 2.4:-63167148 MS.gene047476:CDS
AACATTAGCACATCTATATT+TGG 0.209645 2.4:-63167259 MS.gene047476:CDS
TATATATATTATAGGTTAAC+TGG 0.218480 2.4:-63167636 MS.gene047476:intron
CTTCCAACTCTTTCAATGAT+AGG 0.271660 2.4:-63167342 MS.gene047476:CDS
TTCTTTGGTGCTGCACTTGT+GGG 0.284414 2.4:+63167177 None:intergenic
TGAACCAGGATTAACTTCTT+TGG 0.304162 2.4:+63167162 None:intergenic
ACCTCCCAAGGGCTTTGCTT+TGG 0.312781 2.4:+63167589 None:intergenic
GACACCTGATCACCACTAAT+TGG 0.319561 2.4:-63169013 MS.gene047476:CDS
CCGACGTTCATGGCAAAGTC+TGG 0.342190 2.4:-63169070 MS.gene047476:CDS
TACAATTGCCCTCAGCAAAA+AGG 0.346652 2.4:-63167534 MS.gene047476:CDS
TTTGGATTATGTGAGACTTC+AGG 0.376866 2.4:+63167607 None:intergenic
AGGATGCAATGAAGATTAAC+TGG 0.377747 2.4:-63168749 MS.gene047476:CDS
ATCCGTCGGCATACTAAGTT+TGG 0.389012 2.4:-63168903 MS.gene047476:CDS
CTTCTTTGGTGCTGCACTTG+TGG 0.393725 2.4:+63167176 None:intergenic
CCCTTTGAGACAAAGTTTGA+CGG 0.397840 2.4:-63167202 MS.gene047476:CDS
AATATAGATGTGCTAATGTT+TGG 0.410256 2.4:+63167262 None:intergenic
AAAATCCTTGAATACATTGT+TGG 0.413811 2.4:-63167428 MS.gene047476:CDS
CTGTCTGTTACCGACGTTCA+TGG 0.414559 2.4:-63169080 MS.gene047476:CDS
CACCGTTATTAGCATCAGAT+AGG 0.414739 2.4:+63169183 None:intergenic
CCGTCAAACTTTGTCTCAAA+GGG 0.416058 2.4:+63167202 None:intergenic
GGTGATGAAATTGTTTCCTC+TGG 0.421466 2.4:-63169218 MS.gene047476:CDS
TACTCCAATTAGTGGTGATC+AGG 0.433415 2.4:+63169009 None:intergenic
AGCATGCGCCATGGACGATT+AGG 0.434996 2.4:+63168633 None:intergenic
TCATCTGGAGATATGTTTGT+TGG 0.440275 2.4:-63168927 MS.gene047476:CDS
ACAATCCAACAATGTATTCA+AGG 0.441558 2.4:+63167423 None:intergenic
AACCATCAGTGATGGGAGTT+AGG 0.442469 2.4:+63169285 None:intergenic
CAAGGGAGAAGATGGTTGTA+TGG 0.451305 2.4:-63167080 MS.gene047476:CDS
CACGAACTTACTCCAATTAG+TGG 0.453246 2.4:+63169001 None:intergenic
GGTTATGGCGGCAGCGGAGT+TGG 0.455795 2.4:-63168856 MS.gene047476:CDS
GAGATGGCTGTTTAGTTTCG+AGG 0.456957 2.4:+63169108 None:intergenic
TCATGCAAATTACTTCCAAC+AGG 0.463742 2.4:+63167112 None:intergenic
TTCAAAATGCTATGGTTAAG+AGG 0.466292 2.4:+63167226 None:intergenic
GATAACTAAAGAATCGGTTA+TGG 0.468357 2.4:-63168871 MS.gene047476:CDS
GAGTATTATCCTACGATTGA+AGG 0.470883 2.4:-63168807 MS.gene047476:CDS
TTCCAACTCTTTCAATGATA+GGG 0.473757 2.4:-63167341 MS.gene047476:CDS
ACAAAGACTTCATCAGTATG+AGG 0.477103 2.4:+63169317 None:intergenic
ACCGTCAAACTTTGTCTCAA+AGG 0.497762 2.4:+63167201 None:intergenic
CATTGCATCCTCAACAACCT+TGG 0.498204 2.4:+63168761 None:intergenic
AGAATCGGTTATGGCGGCAG+CGG 0.500580 2.4:-63168862 MS.gene047476:CDS
GATACAATGCAAGGATTGAA+AGG 0.501823 2.4:+63169342 None:intergenic
GGCCCTATCATTGAAAGAGT+TGG 0.504567 2.4:+63167339 None:intergenic
CTGGAAATCTATCATTGGAA+TGG 0.511209 2.4:+63167462 None:intergenic
TCTGCTACCAAGGGAGAAGA+TGG 0.513250 2.4:-63167088 MS.gene047476:CDS
GAATGCTGGAAATCTATCAT+TGG 0.515653 2.4:+63167457 None:intergenic
TGCTAAGATAACTAAAGAAT+CGG 0.520496 2.4:-63168877 MS.gene047476:CDS
TCTCTCTATATTATCTAATG+AGG 0.525248 2.4:+63167489 None:intergenic
TTTGCATGATTCTGCTACCA+AGG 0.526896 2.4:-63167098 MS.gene047476:CDS
ATATTATAGGTTAACTGGGA+TGG 0.530339 2.4:-63167631 MS.gene047476:intron
AGTGGTGATCAGGTGTCTCA+AGG 0.534349 2.4:+63169019 None:intergenic
TAATCCAAAGCAAAGCCCTT+GGG 0.536094 2.4:-63167593 MS.gene047476:CDS
GTATGGGCTGCAATAATTGA+AGG 0.536513 2.4:-63167063 None:intergenic
GAAATTAGTTCAACCTCCCA+AGG 0.540471 2.4:+63167577 None:intergenic
TATGGCGGCAGCGGAGTTGG+CGG 0.543054 2.4:-63168853 MS.gene047476:CDS
ATGAAGATTAACTGGAATTG+TGG 0.543117 2.4:-63168741 MS.gene047476:CDS
CACTAATTGGAGTAAGTTCG+TGG 0.543836 2.4:-63169000 MS.gene047476:CDS
CAAAGGGGTTCAAAATGCTA+TGG 0.546020 2.4:+63167218 None:intergenic
CTCCTAACTCCCATCACTGA+TGG 0.547018 2.4:-63169287 MS.gene047476:CDS
ATAATCCAAAGCAAAGCCCT+TGG 0.548641 2.4:-63167594 MS.gene047476:CDS
CATACAACCATCTTCTCCCT+TGG 0.552659 2.4:+63167081 None:intergenic
TCGTCCATGGCGCATGCTTG+AGG 0.553392 2.4:-63168628 MS.gene047476:intron
ACCGTTATTAGCATCAGATA+GGG 0.553783 2.4:+63169184 None:intergenic
TCTTCACGAACCTCAGTAGG+AGG 0.555844 2.4:+63169251 None:intergenic
TCTCTGCTCCTAATCGTCCA+TGG 0.557073 2.4:-63168641 MS.gene047476:CDS
TTGCATGATTCTGCTACCAA+GGG 0.558661 2.4:-63167097 MS.gene047476:CDS
TCTTTGGTGCTGCACTTGTG+GGG 0.558880 2.4:+63167178 None:intergenic
ATGATGATTATTCAAAGAGA+TGG 0.559089 2.4:-63168692 MS.gene047476:CDS
GGCAATTGTAGATTCAGTGC+AGG 0.560065 2.4:+63167547 None:intergenic
ACCCTATCTGATGCTAATAA+CGG 0.564674 2.4:-63169185 MS.gene047476:CDS
TCCTACTGAGGTTCGTGAAG+AGG 0.565181 2.4:-63169249 MS.gene047476:CDS
AGTATTATCCTACGATTGAA+GGG 0.565286 2.4:-63168806 MS.gene047476:CDS
AAGGGAGAAGATGGTTGTAT+GGG 0.567406 2.4:-63167079 MS.gene047476:CDS
GGTGGATGCCAAGGTTGTTG+AGG 0.569678 2.4:-63168769 MS.gene047476:CDS
TCACAAAACCCTTCAATCGT+AGG 0.570407 2.4:+63168798 None:intergenic
GTGAACACAACCATCAGTGA+TGG 0.572216 2.4:+63169277 None:intergenic
CGACGTTCATGGCAAAGTCT+GGG 0.573902 2.4:-63169069 MS.gene047476:CDS
CCAAATAGCATAAATGAACC+AGG 0.581265 2.4:+63167148 None:intergenic
AGCACCAAAGAAGTTAATCC+TGG 0.583231 2.4:-63167166 MS.gene047476:CDS
ATATGTTTGTTGGAATCCGT+CGG 0.586941 2.4:-63168917 MS.gene047476:CDS
GTTCGTGAAGAGGAACCTAC+AGG 0.587140 2.4:-63169239 MS.gene047476:CDS
GTGGTGATCAGGTGTCTCAA+GGG 0.590017 2.4:+63169020 None:intergenic
TGTTCACAAACCTCCTACTG+AGG 0.591520 2.4:-63169261 MS.gene047476:CDS
AACTAAAGAATCGGTTATGG+CGG 0.592037 2.4:-63168868 MS.gene047476:CDS
GAAACAATTTCATCACCTGT+AGG 0.594064 2.4:+63169224 None:intergenic
AGGGAGTTCCACGGTAGACA+TGG 0.596664 2.4:+63169039 None:intergenic
ATATATATTATAGGTTAACT+GGG 0.596919 2.4:-63167635 MS.gene047476:intron
CTATCCCTTCTTGATGGTCA+CGG 0.597434 2.4:-63169366 None:intergenic
TGTTTAGTTTCGAGGTCAAG+CGG 0.603245 2.4:+63169116 None:intergenic
GGTAAGATGATACATCCTGT+TGG 0.604159 2.4:-63167127 MS.gene047476:CDS
TAGTTTCGAGGTCAAGCGGT+GGG 0.611231 2.4:+63169120 None:intergenic
TGAACACAACCATCAGTGAT+GGG 0.614197 2.4:+63169278 None:intergenic
ATTGATGTCGCGCAAGAACG+AGG 0.619010 2.4:+63169146 None:intergenic
TCCTCTTCACGAACCTCAGT+AGG 0.621000 2.4:+63169248 None:intergenic
AGAGTTGGAAGTAGTTCCTG+TGG 0.623129 2.4:+63167354 None:intergenic
TATACCTCAAGCATGCGCCA+TGG 0.631418 2.4:+63168624 None:intergenic
TATGAGGATGATACAATGCA+AGG 0.632694 2.4:+63169333 None:intergenic
TCCAAAGCAAAGCCCTTGGG+AGG 0.633549 2.4:-63167590 MS.gene047476:CDS
AAATTAGTTCAACCTCCCAA+GGG 0.635245 2.4:+63167578 None:intergenic
CACCAAACTTAGTATGCCGA+CGG 0.637276 2.4:+63168901 None:intergenic
GTAACAGACAGTTCTTGAGA+TGG 0.639997 2.4:+63169092 None:intergenic
AAAAGGCTTAGAGTTGCTCA+GGG 0.650442 2.4:-63167517 MS.gene047476:CDS
TGTCGCGCAAGAACGAGGTA+CGG 0.658162 2.4:+63169151 None:intergenic
GACGAAAAGAAACTCGTCTG+CGG 0.661354 2.4:-63168978 MS.gene047476:CDS
GAGTTTCGCCATGTCTACCG+TGG 0.667413 2.4:-63169047 MS.gene047476:CDS
CGTCAAACTTTGTCTCAAAG+GGG 0.667527 2.4:+63167203 None:intergenic
GGTGTCTCAAGGGAGTTCCA+CGG 0.685317 2.4:+63169030 None:intergenic
CCAGACTTTGCCATGAACGT+CGG 0.685939 2.4:+63169070 None:intergenic
CTATCTGATGCTAATAACGG+TGG 0.699278 2.4:-63169182 MS.gene047476:CDS
TTAGTTTCGAGGTCAAGCGG+TGG 0.704813 2.4:+63169119 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATTATAGGTTAACT+GGG - chr2.4:63168784-63168803 MS.gene047476:CDS 15.0%
!! ATCATATAAATGATTAATGT+TGG - chr2.4:63168737-63168756 MS.gene047476:CDS 15.0%
!! CATCACAATATATATATTAT+AGG - chr2.4:63168775-63168794 MS.gene047476:CDS 15.0%
!! TAAACATATATGTAAACAAA+CGG + chr2.4:63168483-63168502 None:intergenic 15.0%
!! TATATATATTATAGGTTAAC+TGG - chr2.4:63168783-63168802 MS.gene047476:CDS 15.0%
!! AAAAAACAACAACGATATAT+TGG + chr2.4:63168260-63168279 None:intergenic 20.0%
!! AAAAACAACAACGATATATT+GGG + chr2.4:63168259-63168278 None:intergenic 20.0%
!! AATTATTTCAGAAGTTGAAA+TGG - chr2.4:63168439-63168458 MS.gene047476:intron 20.0%
!! ATTATTTCAGAAGTTGAAAT+GGG - chr2.4:63168440-63168459 MS.gene047476:intron 20.0%
!! GTTAAAATTAATATCCTACT+TGG + chr2.4:63168658-63168677 None:intergenic 20.0%
!! TAAAATCAATGAAGTTAATG+TGG + chr2.4:63168396-63168415 None:intergenic 20.0%
!! TATGTATCTATATAAGTCAA+TGG + chr2.4:63168706-63168725 None:intergenic 20.0%
!! TTATGAACAATTATATGCAA+AGG - chr2.4:63167984-63168003 MS.gene047476:intron 20.0%
!! TTCATTGAGAAAAATTCAAT+GGG - chr2.4:63169109-63169128 MS.gene047476:CDS 20.0%
!! TTTATGAAAAAGAAATCATC+TGG - chr2.4:63167477-63167496 MS.gene047476:CDS 20.0%
!! TTTCATTGAGAAAAATTCAA+TGG - chr2.4:63169108-63169127 MS.gene047476:CDS 20.0%
!!! ATTTTTCTCAATGAAATCAT+TGG + chr2.4:63169104-63169123 None:intergenic 20.0%
!!! TTTCTAAAAAAACAAGCAAA+TGG + chr2.4:63168306-63168325 None:intergenic 20.0%
! AAAATCCTTGAATACATTGT+TGG - chr2.4:63168991-63169010 MS.gene047476:CDS 25.0%
! AAATACTCTTAATTCCAAGT+AGG - chr2.4:63168641-63168660 MS.gene047476:CDS 25.0%
! AACATTAGCACATCTATATT+TGG - chr2.4:63169160-63169179 MS.gene047476:CDS 25.0%
! AATATAGATGTGCTAATGTT+TGG + chr2.4:63169160-63169179 None:intergenic 25.0%
! AATTAAAACTGATACATCCT+TGG + chr2.4:63168588-63168607 None:intergenic 25.0%
! ACAATTATATGCAAAGGATA+TGG - chr2.4:63167990-63168009 MS.gene047476:intron 25.0%
! ATGATGATTATTCAAAGAGA+TGG - chr2.4:63167727-63167746 MS.gene047476:intron 25.0%
! ATGTGATAAACGTGATAAAT+TGG - chr2.4:63168171-63168190 MS.gene047476:intron 25.0%
! ATTAAAACTGATACATCCTT+GGG + chr2.4:63168587-63168606 None:intergenic 25.0%
! GAAACTTTATACACTATTGA+TGG - chr2.4:63168416-63168435 MS.gene047476:intron 25.0%
! TAAATACCTAATACATCCAT+AGG + chr2.4:63168080-63168099 None:intergenic 25.0%
! TCTCTCTATATTATCTAATG+AGG + chr2.4:63168933-63168952 None:intergenic 25.0%
! TGCTAAGATAACTAAAGAAT+CGG - chr2.4:63167542-63167561 MS.gene047476:CDS 25.0%
! TGGATGTATTAGGTATTTAT+AGG - chr2.4:63168081-63168100 MS.gene047476:intron 25.0%
!! GATTGTGATACTTTTATTGA+GGG - chr2.4:63169013-63169032 MS.gene047476:CDS 25.0%
!! TTTTTATGAATTGAATGCCT+AGG + chr2.4:63168682-63168701 None:intergenic 25.0%
!!! GGAATTAAGAGTATTTACAA+TGG + chr2.4:63168637-63168656 None:intergenic 25.0%
!!! GGATATTAATTTTAACTCCT+AGG - chr2.4:63168662-63168681 MS.gene047476:CDS 25.0%
ACAATCCAACAATGTATTCA+AGG + chr2.4:63168999-63169018 None:intergenic 30.0%
AGTATTATCCTACGATTGAA+GGG - chr2.4:63167613-63167632 MS.gene047476:CDS 30.0%
ATATTATAGGTTAACTGGGA+TGG - chr2.4:63168788-63168807 MS.gene047476:CDS 30.0%
ATGAAGATTAACTGGAATTG+TGG - chr2.4:63167678-63167697 MS.gene047476:intron 30.0%
GATAACTAAAGAATCGGTTA+TGG - chr2.4:63167548-63167567 MS.gene047476:CDS 30.0%
GTACTATTGAAGATGCTTAA+AGG + chr2.4:63168335-63168354 None:intergenic 30.0%
TGTGGTATGACTCATAAAAA+AGG + chr2.4:63168378-63168397 None:intergenic 30.0%
TTCAAAATGCTATGGTTAAG+AGG + chr2.4:63169196-63169215 None:intergenic 30.0%
TTCCAACTCTTTCAATGATA+GGG - chr2.4:63169078-63169097 MS.gene047476:CDS 30.0%
TTGAGAAAAATTCAATGGGA+AGG - chr2.4:63169113-63169132 MS.gene047476:CDS 30.0%
! AAAGAGATGGTCAGTTTTTA+AGG - chr2.4:63167740-63167759 MS.gene047476:intron 30.0%
! AAGAGATGGTCAGTTTTTAA+GGG - chr2.4:63167741-63167760 MS.gene047476:intron 30.0%
! AATCTATGCTCAAGTGATTT+TGG - chr2.4:63168026-63168045 MS.gene047476:intron 30.0%
! ATCTATGCTCAAGTGATTTT+GGG - chr2.4:63168027-63168046 MS.gene047476:intron 30.0%
! GGATTGTGATACTTTTATTG+AGG - chr2.4:63169012-63169031 MS.gene047476:CDS 30.0%
! TTAGTAGCTTTGCAATCTAT+AGG - chr2.4:63167933-63167952 MS.gene047476:intron 30.0%
AAATTAGTTCAACCTCCCAA+GGG + chr2.4:63168844-63168863 None:intergenic 35.0%
AACCACGCATCATCAAATTA+TGG + chr2.4:63167906-63167925 None:intergenic 35.0%
AACTAAAGAATCGGTTATGG+CGG - chr2.4:63167551-63167570 MS.gene047476:CDS 35.0%
ACAAAGACTTCATCAGTATG+AGG + chr2.4:63167105-63167124 None:intergenic 35.0%
ACCCTATCTGATGCTAATAA+CGG - chr2.4:63167234-63167253 MS.gene047476:CDS 35.0%
ACCGTTATTAGCATCAGATA+GGG + chr2.4:63167238-63167257 None:intergenic 35.0%
AGGATGCAATGAAGATTAAC+TGG - chr2.4:63167670-63167689 MS.gene047476:intron 35.0%
CAAGCAAATGGATAGAAGTA+TGG + chr2.4:63168294-63168313 None:intergenic 35.0%
CCAAATAGCATAAATGAACC+AGG + chr2.4:63169274-63169293 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr2.4:63169271-63169290 MS.gene047476:CDS 35.0%
CTATTGAAGATGCTTAAAGG+AGG + chr2.4:63168332-63168351 None:intergenic 35.0%
CTGGAAATCTATCATTGGAA+TGG + chr2.4:63168960-63168979 None:intergenic 35.0%
CTTCCAACTCTTTCAATGAT+AGG - chr2.4:63169077-63169096 MS.gene047476:CDS 35.0%
GAAAAATTCAATGGGAAGGT+TGG - chr2.4:63169117-63169136 MS.gene047476:CDS 35.0%
GAAACAATTTCATCACCTGT+AGG + chr2.4:63167198-63167217 None:intergenic 35.0%
GAATGCTGGAAATCTATCAT+TGG + chr2.4:63168965-63168984 None:intergenic 35.0%
GAGAAGTTGAATGAATGTTG+TGG - chr2.4:63168222-63168241 MS.gene047476:intron 35.0%
GAGTATTATCCTACGATTGA+AGG - chr2.4:63167612-63167631 MS.gene047476:CDS 35.0%
GATACAATGCAAGGATTGAA+AGG + chr2.4:63167080-63167099 None:intergenic 35.0%
TATGAGGATGATACAATGCA+AGG + chr2.4:63167089-63167108 None:intergenic 35.0%
TCATCTGGAGATATGTTTGT+TGG - chr2.4:63167492-63167511 MS.gene047476:CDS 35.0%
TCATGCAAATTACTTCCAAC+AGG + chr2.4:63169310-63169329 None:intergenic 35.0%
TGAACCAGGATTAACTTCTT+TGG + chr2.4:63169260-63169279 None:intergenic 35.0%
TGTGGAAAAAAGACTGTCTT+GGG + chr2.4:63169050-63169069 None:intergenic 35.0%
TTGGAGCAAGTTAAGTTTGT+AGG + chr2.4:63168559-63168578 None:intergenic 35.0%
TTTGGATTATGTGAGACTTC+AGG + chr2.4:63168815-63168834 None:intergenic 35.0%
! ATATGTTTGTTGGAATCCGT+CGG - chr2.4:63167502-63167521 MS.gene047476:CDS 35.0%
!! TGAAGGGTTTTGTGATTTTG+TGG - chr2.4:63167629-63167648 MS.gene047476:intron 35.0%
!! TTCCATAATTTGATGATGCG+TGG - chr2.4:63167901-63167920 MS.gene047476:intron 35.0%
AAAAAGGCTTAGAGTTGCTC+AGG - chr2.4:63168901-63168920 MS.gene047476:CDS 40.0%
AAAAGGCTTAGAGTTGCTCA+GGG - chr2.4:63168902-63168921 MS.gene047476:CDS 40.0%
AAGGGAGAAGATGGTTGTAT+GGG - chr2.4:63169340-63169359 MS.gene047476:CDS 40.0%
ACCGTCAAACTTTGTCTCAA+AGG + chr2.4:63169221-63169240 None:intergenic 40.0%
AGCACCAAAGAAGTTAATCC+TGG - chr2.4:63169253-63169272 MS.gene047476:CDS 40.0%
ATAATCCAAAGCAAAGCCCT+TGG - chr2.4:63168825-63168844 MS.gene047476:CDS 40.0%
CAAAGGGGTTCAAAATGCTA+TGG + chr2.4:63169204-63169223 None:intergenic 40.0%
CACCGTTATTAGCATCAGAT+AGG + chr2.4:63167239-63167258 None:intergenic 40.0%
CACGAACTTACTCCAATTAG+TGG + chr2.4:63167421-63167440 None:intergenic 40.0%
CACTAATTGGAGTAAGTTCG+TGG - chr2.4:63167419-63167438 MS.gene047476:CDS 40.0%
CCGTCAAACTTTGTCTCAAA+GGG + chr2.4:63169220-63169239 None:intergenic 40.0%
CGTCAAACTTTGTCTCAAAG+GGG + chr2.4:63169219-63169238 None:intergenic 40.0%
CTATCTGATGCTAATAACGG+TGG - chr2.4:63167237-63167256 MS.gene047476:CDS 40.0%
CTGTGGAAAAAAGACTGTCT+TGG + chr2.4:63169051-63169070 None:intergenic 40.0%
GAAATTAGTTCAACCTCCCA+AGG + chr2.4:63168845-63168864 None:intergenic 40.0%
GCTTCTCCTATGGATGTATT+AGG - chr2.4:63168071-63168090 MS.gene047476:intron 40.0%
GGTAAGATGATACATCCTGT+TGG - chr2.4:63169292-63169311 MS.gene047476:CDS 40.0%
GGTGATGAAATTGTTTCCTC+TGG - chr2.4:63167201-63167220 MS.gene047476:CDS 40.0%
GTAACAGACAGTTCTTGAGA+TGG + chr2.4:63167330-63167349 None:intergenic 40.0%
TAATCCAAAGCAAAGCCCTT+GGG - chr2.4:63168826-63168845 MS.gene047476:CDS 40.0%
TACAATTGCCCTCAGCAAAA+AGG - chr2.4:63168885-63168904 MS.gene047476:CDS 40.0%
TACTCCAATTAGTGGTGATC+AGG + chr2.4:63167413-63167432 None:intergenic 40.0%
TCACAAAACCCTTCAATCGT+AGG + chr2.4:63167624-63167643 None:intergenic 40.0%
TGAACACAACCATCAGTGAT+GGG + chr2.4:63167144-63167163 None:intergenic 40.0%
TTGCATGATTCTGCTACCAA+GGG - chr2.4:63169322-63169341 MS.gene047476:CDS 40.0%
TTTGCATGATTCTGCTACCA+AGG - chr2.4:63169321-63169340 MS.gene047476:CDS 40.0%
! AACTCTAAGCCTTTTTGCTG+AGG + chr2.4:63168897-63168916 None:intergenic 40.0%
! ACTCTAAGCCTTTTTGCTGA+GGG + chr2.4:63168896-63168915 None:intergenic 40.0%
! GATACATCCTTGGGAACTTT+TGG + chr2.4:63168578-63168597 None:intergenic 40.0%
! TAGGGTTAGAGTTTTTCCAG+AGG + chr2.4:63167220-63167239 None:intergenic 40.0%
! TGTTTAGTTTCGAGGTCAAG+CGG + chr2.4:63167306-63167325 None:intergenic 40.0%
!! AGGGTTTTGTGATTTTGTGG+TGG - chr2.4:63167632-63167651 MS.gene047476:intron 40.0%
!! CCCTTTGAGACAAAGTTTGA+CGG - chr2.4:63169217-63169236 MS.gene047476:CDS 40.0%
!!! AGGATTTTGCTGTTGAATGC+TGG + chr2.4:63168979-63168998 None:intergenic 40.0%
!!! CAAGACAGTCTTTTTTCCAC+AGG - chr2.4:63169049-63169068 MS.gene047476:CDS 40.0%
ACTTGCTCCAAAAGTTCCCA+AGG - chr2.4:63168568-63168587 MS.gene047476:intron 45.0%
AGAGTTGGAAGTAGTTCCTG+TGG + chr2.4:63169068-63169087 None:intergenic 45.0%
ATCCGTCGGCATACTAAGTT+TGG - chr2.4:63167516-63167535 MS.gene047476:CDS 45.0%
CAAACGGTGAGACAACTTCT+TGG + chr2.4:63168467-63168486 None:intergenic 45.0%
CAACGAAGATGCTTCTCCTA+TGG - chr2.4:63168061-63168080 MS.gene047476:intron 45.0%
CAAGGGAGAAGATGGTTGTA+TGG - chr2.4:63169339-63169358 MS.gene047476:CDS 45.0%
CACCAAACTTAGTATGCCGA+CGG + chr2.4:63167521-63167540 None:intergenic 45.0%
CATACAACCATCTTCTCCCT+TGG + chr2.4:63169341-63169360 None:intergenic 45.0%
CATTGCATCCTCAACAACCT+TGG + chr2.4:63167661-63167680 None:intergenic 45.0%
GACACCTGATCACCACTAAT+TGG - chr2.4:63167406-63167425 MS.gene047476:CDS 45.0%
GACGAAAAGAAACTCGTCTG+CGG - chr2.4:63167441-63167460 MS.gene047476:CDS 45.0%
GGCAATTGTAGATTCAGTGC+AGG + chr2.4:63168875-63168894 None:intergenic 45.0%
GGCCCTATCATTGAAAGAGT+TGG + chr2.4:63169083-63169102 None:intergenic 45.0%
GTGAACACAACCATCAGTGA+TGG + chr2.4:63167145-63167164 None:intergenic 45.0%
TGTTCACAAACCTCCTACTG+AGG - chr2.4:63167158-63167177 MS.gene047476:CDS 45.0%
! AACCATCAGTGATGGGAGTT+AGG + chr2.4:63167137-63167156 None:intergenic 45.0%
! GAGATGGCTGTTTAGTTTCG+AGG + chr2.4:63167314-63167333 None:intergenic 45.0%
!! CATAGGAGAAGCATCTTCGT+TGG + chr2.4:63168063-63168082 None:intergenic 45.0%
!! TTCTTTGGTGCTGCACTTGT+GGG + chr2.4:63169245-63169264 None:intergenic 45.0%
!!! TGATTTTGTGGTGGATGCCA+AGG - chr2.4:63167641-63167660 MS.gene047476:intron 45.0%
AGTGGTGATCAGGTGTCTCA+AGG + chr2.4:63167403-63167422 None:intergenic 50.0%
ATTGATGTCGCGCAAGAACG+AGG + chr2.4:63167276-63167295 None:intergenic 50.0%
CCAGACTTTGCCATGAACGT+CGG + chr2.4:63167352-63167371 None:intergenic 50.0%
CGACGTTCATGGCAAAGTCT+GGG - chr2.4:63167350-63167369 MS.gene047476:CDS 50.0%
CTCCTAACTCCCATCACTGA+TGG - chr2.4:63167132-63167151 MS.gene047476:CDS 50.0%
CTGTCTGTTACCGACGTTCA+TGG - chr2.4:63167339-63167358 MS.gene047476:CDS 50.0%
GTGGTGATCAGGTGTCTCAA+GGG + chr2.4:63167402-63167421 None:intergenic 50.0%
GTTCGTGAAGAGGAACCTAC+AGG - chr2.4:63167180-63167199 MS.gene047476:CDS 50.0%
TAGTTTCGAGGTCAAGCGGT+GGG + chr2.4:63167302-63167321 None:intergenic 50.0%
TATACCTCAAGCATGCGCCA+TGG + chr2.4:63167798-63167817 None:intergenic 50.0%
TCCTACTGAGGTTCGTGAAG+AGG - chr2.4:63167170-63167189 MS.gene047476:CDS 50.0%
TCCTCTTCACGAACCTCAGT+AGG + chr2.4:63167174-63167193 None:intergenic 50.0%
TCTCTGCTCCTAATCGTCCA+TGG - chr2.4:63167778-63167797 MS.gene047476:intron 50.0%
TCTGCTACCAAGGGAGAAGA+TGG - chr2.4:63169331-63169350 MS.gene047476:CDS 50.0%
TCTTCACGAACCTCAGTAGG+AGG + chr2.4:63167171-63167190 None:intergenic 50.0%
TTAGTTTCGAGGTCAAGCGG+TGG + chr2.4:63167303-63167322 None:intergenic 50.0%
!! CTTCTTTGGTGCTGCACTTG+TGG + chr2.4:63169246-63169265 None:intergenic 50.0%
!! TCTTTGGTGCTGCACTTGTG+GGG + chr2.4:63169244-63169263 None:intergenic 50.0%
ACCTCCCAAGGGCTTTGCTT+TGG + chr2.4:63168833-63168852 None:intergenic 55.0%
AGAATCGGTTATGGCGGCAG+CGG - chr2.4:63167557-63167576 MS.gene047476:CDS 55.0%
AGCATGCGCCATGGACGATT+AGG + chr2.4:63167789-63167808 None:intergenic 55.0%
AGGGAGTTCCACGGTAGACA+TGG + chr2.4:63167383-63167402 None:intergenic 55.0%
CCGACGTTCATGGCAAAGTC+TGG - chr2.4:63167349-63167368 MS.gene047476:CDS 55.0%
GAGTTTCGCCATGTCTACCG+TGG - chr2.4:63167372-63167391 MS.gene047476:CDS 55.0%
GGTGGATGCCAAGGTTGTTG+AGG - chr2.4:63167650-63167669 MS.gene047476:intron 55.0%
GGTGTCTCAAGGGAGTTCCA+CGG + chr2.4:63167392-63167411 None:intergenic 55.0%
TCCAAAGCAAAGCCCTTGGG+AGG - chr2.4:63168829-63168848 MS.gene047476:CDS 55.0%
TGTCGCGCAAGAACGAGGTA+CGG + chr2.4:63167271-63167290 None:intergenic 55.0%
TCGTCCATGGCGCATGCTTG+AGG - chr2.4:63167791-63167810 MS.gene047476:intron 60.0%
GGTTATGGCGGCAGCGGAGT+TGG - chr2.4:63167563-63167582 MS.gene047476:CDS 65.0%
TATGGCGGCAGCGGAGTTGG+CGG - chr2.4:63167566-63167585 MS.gene047476:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 63167066 63169375 63167066 ID=MS.gene047476
chr2.4 mRNA 63167066 63169375 63167066 ID=MS.gene047476.t1;Parent=MS.gene047476
chr2.4 exon 63168629 63169375 63168629 ID=MS.gene047476.t1.exon1;Parent=MS.gene047476.t1
chr2.4 CDS 63168629 63169375 63168629 ID=cds.MS.gene047476.t1;Parent=MS.gene047476.t1
chr2.4 exon 63167066 63167644 63167066 ID=MS.gene047476.t1.exon2;Parent=MS.gene047476.t1
chr2.4 CDS 63167066 63167644 63167066 ID=cds.MS.gene047476.t1;Parent=MS.gene047476.t1
Gene Sequence

>MS.gene047476

ATGGTCACGGTCCTTTCAATCCTTGCATTGTATCATCCTCATACTGATGAAGTCTTTGTCAAACTTCTCCTAACTCCCATCACTGATGGTTGTGTTCACAAACCTCCTACTGAGGTTCGTGAAGAGGAACCTACAGGTGATGAAATTGTTTCCTCTGGAAAAACTCTAACCCTATCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTCGTTCTTGCGCGACATCAATCTTCCCACCGCTTGACCTCGAAACTAAACAGCCATCTCAAGAACTGTCTGTTACCGACGTTCATGGCAAAGTCTGGGAGTTTCGCCATGTCTACCGTGGAACTCCCTTGAGACACCTGATCACCACTAATTGGAGTAAGTTCGTGGACGAAAAGAAACTCGTCTGCGGCGATTCTGTAATTTTTATGAAAAAGAAATCATCTGGAGATATGTTTGTTGGAATCCGTCGGCATACTAAGTTTGGTGCTGCTAAGATAACTAAAGAATCGGTTATGGCGGCAGCGGAGTTGGCGGATAAGAATATCGCTTTTGAAGTTGAGTATTATCCTACGATTGAAGGGTTTTGTGATTTTGTGGTGGATGCCAAGGTTGTTGAGGATGCAATGAAGATTAACTGGAATTGTGGAATGAGAATTAAATTTTCTTTGAAGAATGATGATTATTCAAAGAGATGGTCAGTTTTTAAGGGTACAATATCTGCTCTCTCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTATATTTATATTGATCATCTTTTGTATTATATGCAACATTTTTGTTTCATTAATGTTATTACAATTGAATTTGATATAAACGTGATTTTCCATAATTTGATGATGCGTGGTTCATGAAATTAGTAGCTTTGCAATCTATAGGATAAGTTACTATGTGATTCTTTGTGAAATTATGAACAATTATATGCAAAGGATATGGAGTGTATATAGAAAATCTATGCTCAAGTGATTTTGGGCATTTAATAACCAACGAAGATGCTTCTCCTATGGATGTATTAGGTATTTATAGGAAAATGTTTTTCAGACACGCATAAACTCATTAAACAATTTGAGAGAGAAAGAAATAAAAAGAGTTATATGTGATAAACGTGATAAATTGGTAATGTGATAAGAATAGAGAGATAAAATGAGAAGTTGAATGAATGTTGTGGAATTAAGTTGTCCCAATATATCGTTGTTGTTTTTTATTTTATATTACCATACTTCTATCCATTTGCTTGTTTTTTTAGAAACATCCTCCTTTAAGCATCTTCAATAGTACAATTAAATTAGAGTTGCTTTCCTTTTTTATGAGTCATACCACATTAACTTCATTGATTTTAGAAACTTTATACACTATTGATGGAATTATTTCAGAAGTTGAAATGGGTCCAAGAAGTTGTCTCACCGTTTGTTTACATATATGTTTAGATTACATTTTAATAATGTTTAGACAATGTTATCACAACGATTTTTACAAGTACCTACAAACTTAACTTGCTCCAAAAGTTCCCAAGGATGTATCAGTTTTAATTAAGTTTAAGAACTTTTTGAGTAACTTCCATTGTAAATACTCTTAATTCCAAGTAGGATATTAATTTTAACTCCTAGGCATTCAATTCATAAAAAACCATTGACTTATATAGATACATACTATATTTCATATCATATAAATGATTAATGTTGGATTCTCACAATTTTTCATCACAATATATATATTATAGGTTAACTGGGATGGACCTGAAGTCTCACATAATCCAAAGCAAAGCCCTTGGGAGGTTGAACTAATTTCTTATATACCTGCACTGAATCTACAATTGCCCTCAGCAAAAAGGCTTAGAGTTGCTCAGGGTTATACCTCATTAGATAATATAGAGAGATCTTCCATTCCAATGATAGATTTCCAGCATTCAACAGCAAAATCCTTGAATACATTGTTGGATTGTGATACTTTTATTGAGGGCATGCAGAGAGCCCAAGACAGTCTTTTTTCCACAGGAACTACTTCCAACTCTTTCAATGATAGGGCCAATGATTTCATTGAGAAAAATTCAATGGGAAGGTTGGATATTGTGTCAACTGAGCCAAACATTAGCACATCTATATTTGGTGACTTGTCACCTCTTAACCATAGCATTTTGAACCCCTTTGAGACAAAGTTTGACGGTCCCCACAAGTGCAGCACCAAAGAAGTTAATCCTGGTTCATTTATGCTATTTGGTAAGATGATACATCCTGTTGGAAGTAATTTGCATGATTCTGCTACCAAGGGAGAAGATGGTTGTATGGGCTGCAATAATTGA
Protein sequence

>MS.gene047476.t1

MVTVLSILALYHPHTDEVFVKLLLTPITDGCVHKPPTEVREEEPTGDEIVSSGKTLTLSDANNGGAFSVPRSCATSIFPPLDLETKQPSQELSVTDVHGKVWEFRHVYRGTPLRHLITTNWSKFVDEKKLVCGDSVIFMKKKSSGDMFVGIRRHTKFGAAKITKESVMAAAELADKNIAFEVEYYPTIEGFCDFVVDAKVVEDAMKINWNCGMRIKFSLKNDDYSKRWSVFKGTISALSAPNRPWRMLEVNWDGPEVSHNPKQSPWEVELISYIPALNLQLPSAKRLRVAQGYTSLDNIERSSIPMIDFQHSTAKSLNTLLDCDTFIEGMQRAQDSLFSTGTTSNSFNDRANDFIEKNSMGRLDIVSTEPNISTSIFGDLSPLNHSILNPFETKFDGPHKCSTKEVNPGSFMLFGKMIHPVGSNLHDSATKGEDGCMGCNN