AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047626


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047626.t1 MTR_5g017210 92.063 252 13 3 1 250 1 247 7.99e-141 395
MS.gene047626.t1 MTR_3g101810 54.545 209 77 7 25 215 40 248 9.23e-59 187
MS.gene047626.t1 MTR_8g093500 74.725 91 23 0 113 203 87 177 4.49e-46 155
MS.gene047626.t1 MTR_2g100490 57.252 131 48 3 83 206 227 356 4.32e-32 121
MS.gene047626.t1 MTR_8g015450 63.636 99 32 1 112 206 296 394 4.42e-29 114
MS.gene047626.t1 MTR_7g069590 68.966 58 18 0 146 203 32 89 4.35e-23 92.0
MS.gene047626.t1 MTR_5g079500 55.696 79 33 1 126 202 202 280 2.20e-20 88.6
MS.gene047626.t1 MTR_1g106460 53.731 67 31 0 136 202 234 300 1.46e-17 80.9
MS.gene047626.t1 MTR_1g018090 62.963 54 20 0 149 202 283 336 3.87e-17 80.1
MS.gene047626.t1 MTR_5g005110 62.963 54 20 0 149 202 233 286 6.02e-17 79.3
MS.gene047626.t1 MTR_1g106470 51.948 77 32 2 131 202 182 258 9.21e-17 78.2
MS.gene047626.t1 MTR_3g103030 55.556 63 28 0 140 202 229 291 3.61e-16 77.0
MS.gene047626.t1 MTR_4g131160 43.564 101 47 3 121 211 172 272 8.41e-15 73.6
MS.gene047626.t1 MTR_4g131160 43.564 101 47 3 121 211 175 275 8.65e-15 73.6
MS.gene047626.t1 MTR_1g080890 51.429 70 34 0 133 202 186 255 1.08e-14 72.8
MS.gene047626.t1 MTR_1g106480 64.444 45 16 0 158 202 87 131 1.14e-13 67.0
MS.gene047626.t1 MTR_2g067000 46.667 75 33 1 136 203 146 220 1.10e-12 66.6
MS.gene047626.t1 MTR_1g060700 57.407 54 23 0 150 203 357 410 4.50e-12 65.9
MS.gene047626.t1 MTR_4g033295 50.769 65 31 1 141 204 119 183 5.45e-12 63.2
MS.gene047626.t1 MTR_4g033295 50.000 66 32 1 141 205 119 184 1.00e-11 62.4
MS.gene047626.t1 MTR_2g089070 48.148 54 28 0 150 203 146 199 9.29e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047626.t1 AT5G67060 56.771 192 70 3 17 204 4 186 1.08e-50 166
MS.gene047626.t1 AT3G50330 53.917 217 72 6 14 223 4 199 4.86e-49 161
MS.gene047626.t1 AT5G67060 56.284 183 68 2 25 204 1 174 1.36e-47 157
MS.gene047626.t1 AT4G00120 66.667 96 29 1 115 207 87 182 3.66e-39 135
MS.gene047626.t1 AT3G21330 54.472 123 48 1 91 205 212 334 3.80e-38 137
MS.gene047626.t1 AT5G09750 75.556 90 22 0 113 202 92 181 7.38e-35 124
MS.gene047626.t1 AT5G01305 69.492 59 18 0 141 199 38 96 7.40e-22 89.4
MS.gene047626.t1 AT5G43175 50.725 69 34 0 134 202 126 194 7.59e-19 82.8
MS.gene047626.t1 AT4G33880 59.677 62 25 0 141 202 267 328 1.66e-18 84.0
MS.gene047626.t1 AT5G37800 56.164 73 25 1 137 202 191 263 3.69e-18 82.4
MS.gene047626.t1 AT1G66470 67.273 55 18 0 148 202 203 257 5.26e-18 81.6
MS.gene047626.t1 AT1G27740 51.471 68 33 0 135 202 162 229 5.50e-18 80.9
MS.gene047626.t1 AT2G14760 58.065 62 26 0 141 202 238 299 1.65e-17 80.9
MS.gene047626.t1 AT4G30980 40.833 120 69 2 84 202 74 192 5.89e-17 79.0
MS.gene047626.t1 AT2G24260 52.703 74 29 1 135 202 24 97 1.26e-16 77.0
MS.gene047626.t1 AT4G30980 40.833 120 69 2 84 202 122 240 1.27e-16 78.6
MS.gene047626.t1 AT2G24260 52.703 74 29 1 135 202 127 200 3.17e-16 77.4
MS.gene047626.t1 AT5G58010 54.545 66 30 0 137 202 96 161 8.95e-15 72.8
MS.gene047626.t1 AT2G14760 48.000 75 26 1 141 202 238 312 3.48e-14 71.6
MS.gene047626.t1 AT4G09180 43.210 81 45 1 123 202 167 247 8.11e-11 61.2

Find 57 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTGGTTTAATGGTTTC+TGG 0.032783 5.3:-9576843 None:intergenic
CTTTCTCTTTGGTGGTTTAA+TGG 0.129321 5.3:-9576850 None:intergenic
ACAAACAATGATTTCCCTTA+TGG 0.235794 5.3:+9576591 MS.gene047626:CDS
ATCTTCACATTCTTTCTCTT+TGG 0.246196 5.3:-9576861 None:intergenic
AATTACTCTTCTTTGCTTAG+AGG 0.258079 5.3:+9577122 MS.gene047626:CDS
GAGATTGAGAGTGTTGTTAT+TGG 0.283226 5.3:-9576562 None:intergenic
AAAGAATTAGTGAAAGAATA+AGG 0.285456 5.3:+9576925 MS.gene047626:CDS
TTATTGGTGTTGCTATAAAA+AGG 0.298991 5.3:-9576546 None:intergenic
TTGGTGGTTTAATGGTTTCT+GGG 0.314323 5.3:-9576842 None:intergenic
TGCCATTGAATTCTTCTTCT+CGG 0.319338 5.3:-9576766 None:intergenic
TGTCTTGCTGCTACACTTTG+TGG 0.337683 5.3:-9576894 None:intergenic
TTCACATTCTTTCTCTTTGG+TGG 0.352271 5.3:-9576858 None:intergenic
TTCAAACGTTGGAACAAGTT+GGG 0.370508 5.3:+9577042 MS.gene047626:CDS
TTGAATGTTGTTGGGTTTCA+AGG 0.375435 5.3:+9577077 MS.gene047626:CDS
TGAAGACATACTGAAAACTT+TGG 0.382012 5.3:+9576452 MS.gene047626:CDS
GTTTCTGGGTCTATGTTAAT+CGG 0.384832 5.3:-9576828 None:intergenic
TGATGGGATTTGAACTCTGT+TGG 0.387374 5.3:-9576712 None:intergenic
GCCATTGAATTCTTCTTCTC+GGG 0.399282 5.3:-9576765 None:intergenic
ACTAAGCATTTGTTTGGAAG+TGG 0.402015 5.3:-9577174 None:intergenic
AATAGACCTTTGAATGTTGT+TGG 0.403761 5.3:+9577068 MS.gene047626:CDS
GTTACATTTGAGTTTCCATA+AGG 0.409196 5.3:-9576606 None:intergenic
TGGAAGTGGGACCCACCATT+TGG 0.414540 5.3:-9577160 None:intergenic
GATGGGATTTGAACTCTGTT+GGG 0.418955 5.3:-9576711 None:intergenic
GTGTCAACTAAGCATTTGTT+TGG 0.421668 5.3:-9577180 None:intergenic
GAAGACATACTGAAAACTTT+GGG 0.432163 5.3:+9576453 MS.gene047626:CDS
TTCATCACCTTGCCAAATGG+TGG 0.447650 5.3:+9577148 MS.gene047626:CDS
ATAGACCTTTGAATGTTGTT+GGG 0.458742 5.3:+9577069 MS.gene047626:CDS
TGAATTCTTCTTCTCGGGAT+AGG 0.459318 5.3:-9576760 None:intergenic
ATAGGAGAATGAAGAATTGT+TGG 0.474836 5.3:-9576742 None:intergenic
TTACATTTGAGTTTCCATAA+GGG 0.484501 5.3:-9576605 None:intergenic
GTTCAAACGTTGGAACAAGT+TGG 0.486495 5.3:+9577041 MS.gene047626:CDS
GATGACAATGATGATGCAAA+TGG 0.498844 5.3:+9576500 MS.gene047626:CDS
AATTGTTGGTTCTTCCTGAT+GGG 0.499420 5.3:-9576728 None:intergenic
GAATTGTTGGTTCTTCCTGA+TGG 0.503297 5.3:-9576729 None:intergenic
TTGTTCATCACCTTGCCAAA+TGG 0.505893 5.3:+9577145 MS.gene047626:CDS
AGCGACAGCGGTGGTGTCAT+TGG 0.507082 5.3:-9576684 None:intergenic
AACAGAGTTCAAATCCCATC+AGG 0.513806 5.3:+9576714 MS.gene047626:CDS
TGTTGTTGGGTTTCAAGGAG+TGG 0.515205 5.3:+9577082 MS.gene047626:CDS
CTAAGCATTTGTTTGGAAGT+GGG 0.548242 5.3:-9577173 None:intergenic
GAAGAAACAAGTTCAAACGT+TGG 0.554879 5.3:+9577031 MS.gene047626:CDS
TCATCACCTTGCCAAATGGT+GGG 0.555921 5.3:+9577149 MS.gene047626:CDS
TGAAACCCAACAACATTCAA+AGG 0.560330 5.3:-9577074 None:intergenic
TCAAACGTTGGAACAAGTTG+GGG 0.569007 5.3:+9577043 MS.gene047626:CDS
GAGAGAGATGATATTTCGAA+TGG 0.580908 5.3:+9576794 MS.gene047626:CDS
GGTGTCATTGGTTGTTGAAG+AGG 0.589982 5.3:-9576672 None:intergenic
AAAGAATGTGAAGATTTCAA+AGG 0.590303 5.3:+9576869 MS.gene047626:CDS
TGAAGAGGATAAGACAATGG+TGG 0.592428 5.3:-9576657 None:intergenic
TCCCGAGAAGAAGAATTCAA+TGG 0.593246 5.3:+9576764 MS.gene047626:CDS
AGAGATGATATTTCGAATGG+CGG 0.620797 5.3:+9576797 MS.gene047626:CDS
AATGGTGGATTGATCAATGA+AGG 0.629442 5.3:-9576642 None:intergenic
GTGGGACCCACCATTTGGCA+AGG 0.634945 5.3:-9577155 None:intergenic
GGGAAATGTTGAAAGCAACA+TGG 0.641963 5.3:+9576473 MS.gene047626:CDS
CTGTTGGGTTGAAGCGACAG+CGG 0.659647 5.3:-9576696 None:intergenic
TTGGGTTGAAGCGACAGCGG+TGG 0.664890 5.3:-9576693 None:intergenic
ATATTACAAAGATTAGTACC+TGG 0.683168 5.3:+9576948 MS.gene047626:CDS
TTACAAAGATTAGTACCTGG+TGG 0.689802 5.3:+9576951 MS.gene047626:CDS
TGTTGAAGAGGATAAGACAA+TGG 0.700529 5.3:-9576660 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATTAGTGAAAGAATA+AGG + chr5.3:9576925-9576944 MS.gene047626:CDS 20.0%
! AAAAAGGTTCATATGAATCA+GGG - chr5.3:9576533-9576552 None:intergenic 25.0%
! AAAGAATGTGAAGATTTCAA+AGG + chr5.3:9576869-9576888 MS.gene047626:CDS 25.0%
! ATATTACAAAGATTAGTACC+TGG + chr5.3:9576948-9576967 MS.gene047626:CDS 25.0%
! TAAAAAGGTTCATATGAATC+AGG - chr5.3:9576534-9576553 None:intergenic 25.0%
!! TTACATTTGAGTTTCCATAA+GGG - chr5.3:9576608-9576627 None:intergenic 25.0%
!!! TTATTGGTGTTGCTATAAAA+AGG - chr5.3:9576549-9576568 None:intergenic 25.0%
AATAGACCTTTGAATGTTGT+TGG + chr5.3:9577068-9577087 MS.gene047626:CDS 30.0%
AATTACTCTTCTTTGCTTAG+AGG + chr5.3:9577122-9577141 MS.gene047626:CDS 30.0%
ACAAACAATGATTTCCCTTA+TGG + chr5.3:9576591-9576610 MS.gene047626:CDS 30.0%
ATAGACCTTTGAATGTTGTT+GGG + chr5.3:9577069-9577088 MS.gene047626:CDS 30.0%
ATAGGAGAATGAAGAATTGT+TGG - chr5.3:9576745-9576764 None:intergenic 30.0%
ATCTTCACATTCTTTCTCTT+TGG - chr5.3:9576864-9576883 None:intergenic 30.0%
GAAGACATACTGAAAACTTT+GGG + chr5.3:9576453-9576472 MS.gene047626:CDS 30.0%
TGAAGACATACTGAAAACTT+TGG + chr5.3:9576452-9576471 MS.gene047626:CDS 30.0%
! GTTACATTTGAGTTTCCATA+AGG - chr5.3:9576609-9576628 None:intergenic 30.0%
ACTAAGCATTTGTTTGGAAG+TGG - chr5.3:9577177-9577196 None:intergenic 35.0%
AGAGATGATATTTCGAATGG+CGG + chr5.3:9576797-9576816 MS.gene047626:CDS 35.0%
CTAAGCATTTGTTTGGAAGT+GGG - chr5.3:9577176-9577195 None:intergenic 35.0%
GAAGAAACAAGTTCAAACGT+TGG + chr5.3:9577031-9577050 MS.gene047626:CDS 35.0%
GAGAGAGATGATATTTCGAA+TGG + chr5.3:9576794-9576813 MS.gene047626:CDS 35.0%
TGAAACCCAACAACATTCAA+AGG - chr5.3:9577077-9577096 None:intergenic 35.0%
TGCCATTGAATTCTTCTTCT+CGG - chr5.3:9576769-9576788 None:intergenic 35.0%
TGTTGAAGAGGATAAGACAA+TGG - chr5.3:9576663-9576682 None:intergenic 35.0%
TTACAAAGATTAGTACCTGG+TGG + chr5.3:9576951-9576970 MS.gene047626:CDS 35.0%
TTCAAACGTTGGAACAAGTT+GGG + chr5.3:9577042-9577061 MS.gene047626:CDS 35.0%
TTCACATTCTTTCTCTTTGG+TGG - chr5.3:9576861-9576880 None:intergenic 35.0%
TTGAATGTTGTTGGGTTTCA+AGG + chr5.3:9577077-9577096 MS.gene047626:CDS 35.0%
! CTTTCTCTTTGGTGGTTTAA+TGG - chr5.3:9576853-9576872 None:intergenic 35.0%
! GATGACAATGATGATGCAAA+TGG + chr5.3:9576500-9576519 MS.gene047626:CDS 35.0%
! GTTTCTGGGTCTATGTTAAT+CGG - chr5.3:9576831-9576850 None:intergenic 35.0%
! TTGGTGGTTTAATGGTTTCT+GGG - chr5.3:9576845-9576864 None:intergenic 35.0%
! TTTGGTGGTTTAATGGTTTC+TGG - chr5.3:9576846-9576865 None:intergenic 35.0%
!! AATGGTGGATTGATCAATGA+AGG - chr5.3:9576645-9576664 None:intergenic 35.0%
!! AATTGTTGGTTCTTCCTGAT+GGG - chr5.3:9576731-9576750 None:intergenic 35.0%
!! GAGATTGAGAGTGTTGTTAT+TGG - chr5.3:9576565-9576584 None:intergenic 35.0%
AACAGAGTTCAAATCCCATC+AGG + chr5.3:9576714-9576733 MS.gene047626:CDS 40.0%
AGTACCTGGTGGAACAAAAA+TGG + chr5.3:9576962-9576981 MS.gene047626:CDS 40.0%
GATGGGATTTGAACTCTGTT+GGG - chr5.3:9576714-9576733 None:intergenic 40.0%
GCCATTGAATTCTTCTTCTC+GGG - chr5.3:9576768-9576787 None:intergenic 40.0%
GGGAAATGTTGAAAGCAACA+TGG + chr5.3:9576473-9576492 MS.gene047626:CDS 40.0%
GTTCAAACGTTGGAACAAGT+TGG + chr5.3:9577041-9577060 MS.gene047626:CDS 40.0%
TCAAACGTTGGAACAAGTTG+GGG + chr5.3:9577043-9577062 MS.gene047626:CDS 40.0%
TCCCGAGAAGAAGAATTCAA+TGG + chr5.3:9576764-9576783 MS.gene047626:CDS 40.0%
TGAAGAGGATAAGACAATGG+TGG - chr5.3:9576660-9576679 None:intergenic 40.0%
TGAATTCTTCTTCTCGGGAT+AGG - chr5.3:9576763-9576782 None:intergenic 40.0%
TGATGGGATTTGAACTCTGT+TGG - chr5.3:9576715-9576734 None:intergenic 40.0%
TTGTTCATCACCTTGCCAAA+TGG + chr5.3:9577145-9577164 MS.gene047626:CDS 40.0%
!! GAATTGTTGGTTCTTCCTGA+TGG - chr5.3:9576732-9576751 None:intergenic 40.0%
TCATCACCTTGCCAAATGGT+GGG + chr5.3:9577149-9577168 MS.gene047626:CDS 45.0%
TGTCTTGCTGCTACACTTTG+TGG - chr5.3:9576897-9576916 None:intergenic 45.0%
TGTTGTTGGGTTTCAAGGAG+TGG + chr5.3:9577082-9577101 MS.gene047626:CDS 45.0%
TTCATCACCTTGCCAAATGG+TGG + chr5.3:9577148-9577167 MS.gene047626:CDS 45.0%
!! GGTGTCATTGGTTGTTGAAG+AGG - chr5.3:9576675-9576694 None:intergenic 45.0%
!! GTGTCCATTTTTGTTCCACC+AGG - chr5.3:9576969-9576988 None:intergenic 45.0%
TGGAAGTGGGACCCACCATT+TGG - chr5.3:9577163-9577182 None:intergenic 55.0%
!! CTGTTGGGTTGAAGCGACAG+CGG - chr5.3:9576699-9576718 None:intergenic 55.0%
GTGGGACCCACCATTTGGCA+AGG - chr5.3:9577158-9577177 None:intergenic 60.0%
!! AGCGACAGCGGTGGTGTCAT+TGG - chr5.3:9576687-9576706 None:intergenic 60.0%
!! TTGGGTTGAAGCGACAGCGG+TGG - chr5.3:9576696-9576715 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 9576447 9577199 9576447 ID=MS.gene047626
chr5.3 mRNA 9576447 9577199 9576447 ID=MS.gene047626.t1;Parent=MS.gene047626
chr5.3 exon 9576447 9577199 9576447 ID=MS.gene047626.t1.exon1;Parent=MS.gene047626.t1
chr5.3 CDS 9576447 9577199 9576447 ID=cds.MS.gene047626.t1;Parent=MS.gene047626.t1
Gene Sequence

>MS.gene047626

ATGGATGAAGACATACTGAAAACTTTGGGAAATGTTGAAAGCAACATGGACATGATGACAATGATGATGCAAATGGAAAAGTTCCCTGATTCATATGAACCTTTTTATAGCAACACCAATAACAACACTCTCAATCTCAATCTCACAAACAATGATTTCCCTTATGGAAACTCAAATGTAACTTCATCACTCACACCTTCATTGATCAATCCACCATTGTCTTATCCTCTTCAACAACCAATGACACCACCGCTGTCGCTTCAACCCAACAGAGTTCAAATCCCATCAGGAAGAACCAACAATTCTTCATTCTCCTATCCCGAGAAGAAGAATTCAATGGCAGCAATGAGAGAGATGATATTTCGAATGGCGGTTATGCAACCGATTAACATAGACCCAGAAACCATTAAACCACCAAAGAGAAAGAATGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCAAGACATAGAAGAGAAAGAATTAGTGAAAGAATAAGGATATTACAAAGATTAGTACCTGGTGGAACAAAAATGGACACTGCTTCTATGTTAGATGAAGCAATACATTATGTGAAGTTTTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGGGCAAATAGACCTTTGAATGTTGTTGGGTTTCAAGGAGTGGTTTCTAATGCTAATTATAATTACTCTTCTTTGCTTAGAGGTTGTTCATCACCTTGCCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGA

Protein sequence

>MS.gene047626.t1

MDEDILKTLGNVESNMDMMTMMMQMEKFPDSYEPFYSNTNNNTLNLNLTNNDFPYGNSNVTSSLTPSLINPPLSYPLQQPMTPPLSLQPNRVQIPSGRTNNSSFSYPEKKNSMAAMREMIFRMAVMQPINIDPETIKPPKRKNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQTLEQVGANRPLNVVGFQGVVSNANYNYSSLLRGCSSPCQMVGPTSKQMLS