AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047640.t1 MTR_5g017470 94.444 288 15 1 1 287 1 288 0.0 544
MS.gene047640.t1 MTR_5g010680 46.484 256 111 6 2 241 17 262 2.80e-64 206
MS.gene047640.t1 MTR_3g101870 42.366 262 118 6 2 234 17 274 2.09e-56 186
MS.gene047640.t1 MTR_1g100777 45.455 209 76 3 2 194 11 197 6.36e-52 172
MS.gene047640.t1 MTR_0020s0020 44.651 215 81 2 2 191 26 227 1.94e-51 172
MS.gene047640.t1 MTR_5g089170 40.164 244 117 6 2 235 18 242 5.62e-49 163
MS.gene047640.t1 MTR_7g091370 43.850 187 81 1 3 189 16 178 5.76e-48 160
MS.gene047640.t1 MTR_5g029680 46.237 186 64 3 1 181 24 178 4.14e-44 156
MS.gene047640.t1 MTR_7g095680 69.474 95 29 0 6 100 46 140 1.47e-43 152
MS.gene047640.t1 MTR_6g043060 58.407 113 44 1 8 117 21 133 1.62e-42 149
MS.gene047640.t1 MTR_8g087540 39.720 214 91 4 5 217 8 184 3.99e-41 147
MS.gene047640.t1 MTR_1g071360 63.636 99 36 0 7 105 30 128 5.57e-40 142
MS.gene047640.t1 MTR_1g071360 63.636 99 36 0 7 105 27 125 9.55e-40 142
MS.gene047640.t1 MTR_4g077970 41.500 200 85 4 9 208 12 179 9.85e-39 142
MS.gene047640.t1 MTR_8g083100 56.757 111 45 1 10 117 23 133 1.40e-38 141
MS.gene047640.t1 MTR_2g100680 62.136 103 39 0 7 109 29 131 1.50e-38 141
MS.gene047640.t1 MTR_3g104550 56.481 108 45 1 6 113 43 148 1.31e-37 137
MS.gene047640.t1 MTR_1g061170 38.208 212 114 4 3 212 43 239 1.54e-37 138
MS.gene047640.t1 MTR_2g100670 53.846 117 51 1 6 119 30 146 1.79e-37 135
MS.gene047640.t1 MTR_2g017890 39.894 188 80 4 9 193 15 172 2.29e-37 136
MS.gene047640.t1 MTR_1g102860 35.961 203 116 3 6 208 37 225 6.26e-37 134
MS.gene047640.t1 MTR_6g086805 58.824 102 42 0 1 102 3 104 8.63e-37 134
MS.gene047640.t1 MTR_2g100670 53.846 117 51 1 6 119 138 254 1.64e-36 135
MS.gene047640.t1 MTR_4g088900 36.139 202 103 5 6 203 30 209 5.31e-35 125
MS.gene047640.t1 MTR_8g105780 44.382 178 65 5 8 181 21 168 2.62e-34 128
MS.gene047640.t1 MTR_2g085115 55.752 113 31 5 70 179 39 135 8.86e-25 97.1
MS.gene047640.t1 MTR_5g047190 45.361 97 46 3 17 106 1 97 5.95e-19 80.5
MS.gene047640.t1 MTR_4g045757 60.345 58 23 0 9 66 14 71 3.16e-18 77.8
MS.gene047640.t1 MTR_3g091000 61.538 52 19 1 154 204 27 78 2.79e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047640.t1 AT4G36990 50.505 297 117 7 2 287 7 284 2.06e-83 252
MS.gene047640.t1 AT5G62020 49.756 205 97 3 2 202 16 218 1.12e-55 182
MS.gene047640.t1 AT4G11660 42.222 270 115 8 2 233 52 318 5.50e-54 180
MS.gene047640.t1 AT1G46264 44.444 216 91 5 4 197 29 237 1.04e-53 178
MS.gene047640.t1 AT2G41690 45.989 187 89 3 7 190 38 215 3.24e-48 161
MS.gene047640.t1 AT1G32330 48.066 181 60 3 6 181 34 185 6.84e-47 164
MS.gene047640.t1 AT1G32330 48.066 181 60 3 6 181 34 185 7.21e-47 164
MS.gene047640.t1 AT4G17750 69.388 98 30 0 5 102 48 145 3.40e-44 157
MS.gene047640.t1 AT2G26150 40.306 196 93 2 5 194 40 217 3.92e-42 148
MS.gene047640.t1 AT2G26150 40.306 196 93 2 5 194 40 217 3.92e-42 148
MS.gene047640.t1 AT3G22830 38.318 214 124 3 5 217 57 263 4.94e-42 149
MS.gene047640.t1 AT4G13980 62.000 100 38 0 10 109 24 123 6.37e-41 147
MS.gene047640.t1 AT3G02990 40.234 256 114 8 5 258 19 237 9.54e-41 147
MS.gene047640.t1 AT3G24520 38.426 216 83 4 8 216 16 188 1.23e-39 141
MS.gene047640.t1 AT5G16820 69.565 92 28 0 9 100 27 118 2.28e-39 144
MS.gene047640.t1 AT5G16820 69.565 92 28 0 9 100 27 118 2.28e-39 144
MS.gene047640.t1 AT5G43840 56.881 109 47 0 5 113 15 123 9.40e-39 137
MS.gene047640.t1 AT3G51910 58.654 104 42 1 6 109 26 128 4.24e-38 135
MS.gene047640.t1 AT5G03720 42.246 187 82 3 9 189 55 221 5.18e-38 139
MS.gene047640.t1 AT5G03720 42.246 187 82 3 9 189 55 221 5.18e-38 139
MS.gene047640.t1 AT5G54070 60.870 92 36 0 8 99 70 161 1.13e-36 133
MS.gene047640.t1 AT3G63350 57.732 97 41 0 3 99 22 118 5.44e-35 127
MS.gene047640.t1 AT4G18880 55.172 116 52 0 5 120 11 126 2.53e-33 126
MS.gene047640.t1 AT1G67970 57.273 110 44 2 8 116 18 125 2.68e-33 125
MS.gene047640.t1 AT5G45710 53.279 122 50 2 5 119 9 130 9.95e-33 123
MS.gene047640.t1 AT5G45710 53.279 122 50 2 5 119 9 130 9.95e-33 123
MS.gene047640.t1 AT4G18870 44.211 95 53 0 2 96 139 233 2.52e-22 94.4
MS.gene047640.t1 AT4G18870 45.745 94 46 3 5 96 9 99 5.78e-20 87.8
MS.gene047640.t1 AT1G77570 40.909 88 47 3 10 93 27 113 1.19e-13 67.4

Find 51 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTGTGCCAGATAAA+TGG 0.251473 5.3:+9772451 MS.gene047640:CDS
TTTGCAAATTCCCATTTATC+TGG 0.289664 5.3:-9772462 None:intergenic
TTGTTGTGCTTAAAATATTT+AGG 0.304096 5.3:-9771319 None:intergenic
GTTTGAAATTGTTTGGTGTT+TGG 0.347895 5.3:+9772919 MS.gene047640:CDS
GGATCCTGCTCCGTCGTTGC+CGG 0.352068 5.3:-9772613 None:intergenic
ATTTATCTGGCACAAGCTTT+CGG 0.357484 5.3:-9772449 None:intergenic
CCGTCGTTGCCGGAGTTTGA+TGG 0.360239 5.3:-9772603 None:intergenic
CGAAACCTTGAGTTGTGAAT+TGG 0.361410 5.3:+9772743 MS.gene047640:CDS
GTTGTTGTTTCCACTGATCC+AGG 0.401148 5.3:-9772666 None:intergenic
ATTATGCGATCGATTTGATC+TGG 0.408543 5.3:-9772828 None:intergenic
GAGGCCCACCAAAGCTCAAT+TGG 0.411379 5.3:-9772998 None:intergenic
AGAGATTGTTTGAAATTGTT+TGG 0.415249 5.3:+9772912 MS.gene047640:CDS
TGAGTTGGAGGAAGAGGTTG+AGG 0.450915 5.3:-9772637 None:intergenic
AAAGCTTGTGCCAGATAAAT+GGG 0.453955 5.3:+9772452 MS.gene047640:CDS
CTCGATAAGTTAACACATTG+AGG 0.469755 5.3:-9772693 None:intergenic
AATGTCGCAGCGGTCGGCGC+CGG 0.469760 5.3:+9771171 None:intergenic
TGGAGAGTTGTCATTGATGC+TGG 0.489530 5.3:-9772583 None:intergenic
ACAACCATCGTCACAACCCC+TGG 0.491091 5.3:+9772548 MS.gene047640:CDS
TGTGAAGACCAATTGAGCTT+TGG 0.492259 5.3:+9772990 MS.gene047640:CDS
TCTAAAAGACAATTTAAATG+TGG 0.501767 5.3:+9772803 MS.gene047640:CDS
TGGTGGGCCTCGAGCTAAAG+TGG 0.502290 5.3:+9773010 MS.gene047640:CDS
CTAAAAGACAATTTAAATGT+GGG 0.505450 5.3:+9772804 MS.gene047640:CDS
TATCGGTGAGGAGGTGTTTG+TGG 0.509612 5.3:-9772503 None:intergenic
TTGTGACGATGGTTGTGAGA+TGG 0.514637 5.3:-9772541 None:intergenic
AACTCCGGCAACGACGGAGC+AGG 0.525429 5.3:+9772609 MS.gene047640:CDS
GTTGGAGGAAGAGGTTGAGG+TGG 0.526221 5.3:-9772634 None:intergenic
GAAAGAAACAATAAGAAGAG+AGG 0.527898 5.3:+9772966 MS.gene047640:CDS
TTGTTGTTTCCACTGATCCA+GGG 0.529235 5.3:-9772665 None:intergenic
GAAGACCAATTGAGCTTTGG+TGG 0.529527 5.3:+9772993 MS.gene047640:CDS
GCTTCCAGGGGTTGTGACGA+TGG 0.537408 5.3:-9772552 None:intergenic
ACGTGCCAATTCACAACTCA+AGG 0.543571 5.3:-9772748 None:intergenic
CGACGGATGATGTGATATCA+TGG 0.550146 5.3:+9771236 MS.gene047640:CDS
GTCTTCAACAAGAACGGCGC+CGG 0.557423 5.3:-9771190 None:intergenic
GTTGGTGAAGTTGAAGAAAG+TGG 0.559506 5.3:+9772888 MS.gene047640:CDS
AAAGAAACAATGCGAAGAGT+TGG 0.565353 5.3:+9772773 MS.gene047640:CDS
GACAACTCTCCATCAAACTC+CGG 0.573360 5.3:+9772594 MS.gene047640:CDS
CCATCAAACTCCGGCAACGA+CGG 0.576121 5.3:+9772603 MS.gene047640:CDS
GTTGTGAACATGATGAAAAG+TGG 0.578148 5.3:+9773065 MS.gene047640:CDS
CTTGTTGAAGACGTATCAGA+TGG 0.591002 5.3:+9771201 MS.gene047640:CDS
TTGTTACCCACTTTAGCTCG+AGG 0.599182 5.3:-9773017 None:intergenic
GGCGTTTGATATCGGTGAGG+AGG 0.622756 5.3:-9772512 None:intergenic
GAATCTGTTCAAAATGCGGT+TGG 0.630106 5.3:+9772870 MS.gene047640:CDS
GTGATATCATGGAGTGAGAG+TGG 0.634834 5.3:+9771247 MS.gene047640:CDS
AAGACCAATTGAGCTTTGGT+GGG 0.635945 5.3:+9772994 MS.gene047640:CDS
GGTGGGCCTCGAGCTAAAGT+GGG 0.638586 5.3:+9773011 MS.gene047640:CDS
TTGTGAATCTGTTCAAAATG+CGG 0.658508 5.3:+9772866 MS.gene047640:CDS
GAACATGATGAAAAGTGGCA+AGG 0.659767 5.3:+9773070 MS.gene047640:CDS
TGATACGTCTTCAACAAGAA+CGG 0.678180 5.3:-9771196 None:intergenic
GCAAACGAAAATTTCAAACG+AGG 0.682155 5.3:+9772480 MS.gene047640:CDS
TGCGGCGTTTGATATCGGTG+AGG 0.683931 5.3:-9772515 None:intergenic
GTTGAAGACGTATCAGATGG+TGG 0.717769 5.3:+9771204 MS.gene047640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATTATTTTTATTTTAA+TGG + chr5.3:9771706-9771725 MS.gene047640:intron 0.0%
!!! TATTTTAACAAATAGTAAAT+AGG - chr5.3:9771866-9771885 None:intergenic 10.0%
!!! TATTTTCAAAATAAAAACAT+TGG - chr5.3:9771480-9771499 None:intergenic 10.0%
!! AAATTATAATACACTTTCTT+AGG - chr5.3:9772171-9772190 None:intergenic 15.0%
!! AATTATAATACACTTTCTTA+GGG - chr5.3:9772170-9772189 None:intergenic 15.0%
!!! AATTACTTTTTAGCAATTTT+TGG + chr5.3:9771502-9771521 MS.gene047640:intron 15.0%
!!! ATTACTTTTTAGCAATTTTT+GGG + chr5.3:9771503-9771522 MS.gene047640:intron 15.0%
!!! TGTTCAAATATTTTGATTTT+AGG + chr5.3:9771748-9771767 MS.gene047640:intron 15.0%
!! AGTTATCTTAAACTAATCAA+AGG - chr5.3:9771449-9771468 None:intergenic 20.0%
!! CTAAAAGACAATTTAAATGT+GGG + chr5.3:9772804-9772823 MS.gene047640:CDS 20.0%
!! TCTAAAAGACAATTTAAATG+TGG + chr5.3:9772803-9772822 MS.gene047640:CDS 20.0%
!! TTGTTGTGCTTAAAATATTT+AGG - chr5.3:9771322-9771341 None:intergenic 20.0%
!!! TCAATTATTTTAGTGTTCAA+TGG + chr5.3:9772305-9772324 MS.gene047640:intron 20.0%
!!! TTACTTTTTAGCAATTTTTG+GGG + chr5.3:9771504-9771523 MS.gene047640:intron 20.0%
! AAAATAAAAACATTGGCAGT+TGG - chr5.3:9771473-9771492 None:intergenic 25.0%
!! AGAGATTGTTTGAAATTGTT+TGG + chr5.3:9772912-9772931 MS.gene047640:CDS 25.0%
!! ATTTTATACTTGCTTGTTTG+TGG - chr5.3:9771569-9771588 None:intergenic 25.0%
!! CTACCAAGTAAATTCATTTT+CGG - chr5.3:9771933-9771952 None:intergenic 25.0%
!!! GATGATTTACTTTTAGTGTT+CGG + chr5.3:9772268-9772287 MS.gene047640:intron 25.0%
!!! TAGGTTTTATCTTAACACTT+TGG + chr5.3:9772380-9772399 MS.gene047640:intron 25.0%
ACACCGAAAATGAATTTACT+TGG + chr5.3:9771927-9771946 MS.gene047640:intron 30.0%
AGAAAACGTGCTAATATTTG+AGG - chr5.3:9772356-9772375 None:intergenic 30.0%
CAATACTTTCCTTCCAATTA+GGG - chr5.3:9771838-9771857 None:intergenic 30.0%
CACATCTTTACAATTATGCA+CGG - chr5.3:9772239-9772258 None:intergenic 30.0%
CATGTTTACGAAGAACTAAA+AGG - chr5.3:9771402-9771421 None:intergenic 30.0%
GAAAACGTGCTAATATTTGA+GGG - chr5.3:9772355-9772374 None:intergenic 30.0%
GAAAGAAACAATAAGAAGAG+AGG + chr5.3:9772966-9772985 MS.gene047640:CDS 30.0%
TGAGAAAATGCTAATTACCA+TGG + chr5.3:9772406-9772425 MS.gene047640:intron 30.0%
TTGGTAGATCACAATCAATT+AGG + chr5.3:9771946-9771965 MS.gene047640:intron 30.0%
TTGTGAATCTGTTCAAAATG+CGG + chr5.3:9772866-9772885 MS.gene047640:CDS 30.0%
TTTGCAAATTCCCATTTATC+TGG - chr5.3:9772465-9772484 None:intergenic 30.0%
! AGTGTTCAATGGTTTACTTT+TGG + chr5.3:9772316-9772335 MS.gene047640:intron 30.0%
! ATTAGCACGTTTTCTTTTCT+AGG + chr5.3:9772361-9772380 MS.gene047640:intron 30.0%
!! ATTTACTTTTAGTGTTCGGA+TGG + chr5.3:9772272-9772291 MS.gene047640:intron 30.0%
!! GTTTGAAATTGTTTGGTGTT+TGG + chr5.3:9772919-9772938 MS.gene047640:CDS 30.0%
!!! AATTTTTGGGGTTCATAACA+AGG + chr5.3:9771516-9771535 MS.gene047640:intron 30.0%
!!! CTTTTAGTTCTTCGTAAACA+TGG + chr5.3:9771400-9771419 MS.gene047640:intron 30.0%
AAAGAAACAATGCGAAGAGT+TGG + chr5.3:9772773-9772792 MS.gene047640:CDS 35.0%
AAAGCTTGTGCCAGATAAAT+GGG + chr5.3:9772452-9772471 MS.gene047640:CDS 35.0%
AGTAGAATACCCTAATTGGA+AGG + chr5.3:9771826-9771845 MS.gene047640:intron 35.0%
ATATGTTGTTCACACAAGCA+AGG + chr5.3:9771592-9771611 MS.gene047640:intron 35.0%
ATTTATCTGGCACAAGCTTT+CGG - chr5.3:9772452-9772471 None:intergenic 35.0%
CCAATACTTTCCTTCCAATT+AGG - chr5.3:9771839-9771858 None:intergenic 35.0%
CTCGATAAGTTAACACATTG+AGG - chr5.3:9772696-9772715 None:intergenic 35.0%
GCAAACGAAAATTTCAAACG+AGG + chr5.3:9772480-9772499 MS.gene047640:CDS 35.0%
GGACATCGACGTTAAAAAAT+TGG - chr5.3:9772218-9772237 None:intergenic 35.0%
GTTGTGAACATGATGAAAAG+TGG + chr5.3:9773065-9773084 MS.gene047640:CDS 35.0%
TAAAAGGAGAGAGGGAAATA+AGG - chr5.3:9771386-9771405 None:intergenic 35.0%
TGATACGTCTTCAACAAGAA+CGG - chr5.3:9771199-9771218 None:intergenic 35.0%
! ACCATGGTTTTTGAATTTGC+AGG + chr5.3:9772422-9772441 MS.gene047640:intron 35.0%
! CCAATTAGGGTATTCTACTT+CGG - chr5.3:9771825-9771844 None:intergenic 35.0%
! CCATGGTTTTTGAATTTGCA+GGG + chr5.3:9772423-9772442 MS.gene047640:intron 35.0%
! CCTAATTGGAAGGAAAGTAT+TGG + chr5.3:9771836-9771855 MS.gene047640:intron 35.0%
! GGTTTTCAATCTTTTCGTGT+AGG - chr5.3:9771797-9771816 None:intergenic 35.0%
!! ATTATGCGATCGATTTGATC+TGG - chr5.3:9772831-9772850 None:intergenic 35.0%
!!! GAAACATGCTGATTTTGCAA+GGG + chr5.3:9771288-9771307 MS.gene047640:CDS 35.0%
ACGAAGAACTAAAAGGAGAG+AGG - chr5.3:9771395-9771414 None:intergenic 40.0%
CCCTGCAAATTCAAAAACCA+TGG - chr5.3:9772426-9772445 None:intergenic 40.0%
CCGAAGTAGAATACCCTAAT+TGG + chr5.3:9771822-9771841 MS.gene047640:intron 40.0%
CGAAGAACTAAAAGGAGAGA+GGG - chr5.3:9771394-9771413 None:intergenic 40.0%
CTTGTTGAAGACGTATCAGA+TGG + chr5.3:9771201-9771220 MS.gene047640:CDS 40.0%
GAAAGCTTGTGCCAGATAAA+TGG + chr5.3:9772451-9772470 MS.gene047640:CDS 40.0%
GAACATGATGAAAAGTGGCA+AGG + chr5.3:9773070-9773089 MS.gene047640:CDS 40.0%
GAATCTGTTCAAAATGCGGT+TGG + chr5.3:9772870-9772889 MS.gene047640:CDS 40.0%
GGCTGCAAAACATACAAGAA+TGG - chr5.3:9771428-9771447 None:intergenic 40.0%
TGTGAAGACCAATTGAGCTT+TGG + chr5.3:9772990-9773009 MS.gene047640:CDS 40.0%
! AAGACCAATTGAGCTTTGGT+GGG + chr5.3:9772994-9773013 MS.gene047640:CDS 40.0%
! CGAAACCTTGAGTTGTGAAT+TGG + chr5.3:9772743-9772762 MS.gene047640:CDS 40.0%
! GTTGGTGAAGTTGAAGAAAG+TGG + chr5.3:9772888-9772907 MS.gene047640:CDS 40.0%
!! ATGGTTGTGAGATGGTTTTG+CGG - chr5.3:9772536-9772555 None:intergenic 40.0%
!! TTGTTGTTTCCACTGATCCA+GGG - chr5.3:9772668-9772687 None:intergenic 40.0%
!!! GGAAACATGCTGATTTTGCA+AGG + chr5.3:9771287-9771306 MS.gene047640:CDS 40.0%
!!! GTGGTGAAAGTTTTGTTGTG+TGG + chr5.3:9771266-9771285 MS.gene047640:CDS 40.0%
!!! GTTTTTTGAGTTGGAGGAAG+AGG - chr5.3:9772646-9772665 None:intergenic 40.0%
!!! TGATCCAGGGTTTTTTGAGT+TGG - chr5.3:9772655-9772674 None:intergenic 40.0%
ACGTGCCAATTCACAACTCA+AGG - chr5.3:9772751-9772770 None:intergenic 45.0%
CGACGGATGATGTGATATCA+TGG + chr5.3:9771236-9771255 MS.gene047640:CDS 45.0%
CTCAAAAAACCCTGGATCAG+TGG + chr5.3:9772656-9772675 MS.gene047640:CDS 45.0%
GACAACTCTCCATCAAACTC+CGG + chr5.3:9772594-9772613 MS.gene047640:CDS 45.0%
GGGTATTCTACTTCGGTAGA+AGG - chr5.3:9771818-9771837 None:intergenic 45.0%
GTGATATCATGGAGTGAGAG+TGG + chr5.3:9771247-9771266 MS.gene047640:CDS 45.0%
GTTGAAGACGTATCAGATGG+TGG + chr5.3:9771204-9771223 MS.gene047640:CDS 45.0%
TCCTCCAACTCAAAAAACCC+TGG + chr5.3:9772648-9772667 MS.gene047640:CDS 45.0%
TGGAGAGTTGTCATTGATGC+TGG - chr5.3:9772586-9772605 None:intergenic 45.0%
TTGTGACGATGGTTGTGAGA+TGG - chr5.3:9772544-9772563 None:intergenic 45.0%
! GAAGACCAATTGAGCTTTGG+TGG + chr5.3:9772993-9773012 MS.gene047640:CDS 45.0%
! GGTTTTGCGGCGTTTGATAT+CGG - chr5.3:9772523-9772542 None:intergenic 45.0%
! TTGTTACCCACTTTAGCTCG+AGG - chr5.3:9773020-9773039 None:intergenic 45.0%
!! GTTGTTGTTTCCACTGATCC+AGG - chr5.3:9772669-9772688 None:intergenic 45.0%
!!! CTGGTTTTTTCAGCTTCCAG+GGG - chr5.3:9772567-9772586 None:intergenic 45.0%
!!! GATGGTGGATGATTTTGCGA+CGG + chr5.3:9771219-9771238 MS.gene047640:CDS 45.0%
!!! GCTGGTTTTTTCAGCTTCCA+GGG - chr5.3:9772568-9772587 None:intergenic 45.0%
!!! TCCAGGGTTTTTTGAGTTGG+AGG - chr5.3:9772652-9772671 None:intergenic 45.0%
!!! TGCTGGTTTTTTCAGCTTCC+AGG - chr5.3:9772569-9772588 None:intergenic 45.0%
! TGAGTTGGAGGAAGAGGTTG+AGG - chr5.3:9772640-9772659 None:intergenic 50.0%
!! TATCGGTGAGGAGGTGTTTG+TGG - chr5.3:9772506-9772525 None:intergenic 50.0%
ACAACCATCGTCACAACCCC+TGG + chr5.3:9772548-9772567 MS.gene047640:CDS 55.0%
CCATCAAACTCCGGCAACGA+CGG + chr5.3:9772603-9772622 MS.gene047640:CDS 55.0%
GAGGCCCACCAAAGCTCAAT+TGG - chr5.3:9773001-9773020 None:intergenic 55.0%
GGCGTTTGATATCGGTGAGG+AGG - chr5.3:9772515-9772534 None:intergenic 55.0%
GTCTTCAACAAGAACGGCGC+CGG - chr5.3:9771193-9771212 None:intergenic 55.0%
GTTGGAGGAAGAGGTTGAGG+TGG - chr5.3:9772637-9772656 None:intergenic 55.0%
TGCGGCGTTTGATATCGGTG+AGG - chr5.3:9772518-9772537 None:intergenic 55.0%
CCGTCGTTGCCGGAGTTTGA+TGG - chr5.3:9772606-9772625 None:intergenic 60.0%
GCTTCCAGGGGTTGTGACGA+TGG - chr5.3:9772555-9772574 None:intergenic 60.0%
GGTGGGCCTCGAGCTAAAGT+GGG + chr5.3:9773011-9773030 MS.gene047640:CDS 60.0%
! TGGTGGGCCTCGAGCTAAAG+TGG + chr5.3:9773010-9773029 MS.gene047640:CDS 60.0%
AACTCCGGCAACGACGGAGC+AGG + chr5.3:9772609-9772628 MS.gene047640:CDS 65.0%
GGATCCTGCTCCGTCGTTGC+CGG - chr5.3:9772616-9772635 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 9771172 9773103 9771172 ID=MS.gene047640
chr5.3 mRNA 9771172 9773103 9771172 ID=MS.gene047640.t1;Parent=MS.gene047640
chr5.3 exon 9771172 9771375 9771172 ID=MS.gene047640.t1.exon1;Parent=MS.gene047640.t1
chr5.3 CDS 9771172 9771375 9771172 ID=cds.MS.gene047640.t1;Parent=MS.gene047640.t1
chr5.3 exon 9772444 9773103 9772444 ID=MS.gene047640.t1.exon2;Parent=MS.gene047640.t1
chr5.3 CDS 9772444 9773103 9772444 ID=cds.MS.gene047640.t1;Parent=MS.gene047640.t1
Gene Sequence

>MS.gene047640

ATGTCGCAGCGGTCGGCGCCGGCGCCGTTCTTGTTGAAGACGTATCAGATGGTGGATGATTTTGCGACGGATGATGTGATATCATGGAGTGAGAGTGGTGAAAGTTTTGTTGTGTGGAAACATGCTGATTTTGCAAGGGATTTGCTTCCTAAATATTTTAAGCACAACAATTTCTCTAGCTTTGTTCGTCAGCTCAATACTTATGTAAGTTCCTTATTTCCCTCTCTCCTTTTAGTTCTTCGTAAACATGGTTCCATTCTTGTATGTTTTGCAGCCTTTGATTAGTTTAAGATAACTTCCAACTGCCAATGTTTTTATTTTGAAAATAGAAATTACTTTTTAGCAATTTTTGGGGTTCATAACAAGGTAATTTGTTTGTTTATGCTTTATTTTTCCACAAACAAGCAAGTATAAAATGACATATGTTGTTCACACAAGCAAGGATCACAACTACAAATCTAAAGTATTTAAATACACTAATATAAAATAAAAATATTAAAAGAAGATAAAATTAAATGATGTTAATTGATGTGAATAAATTATTTTTATTTTAATGGATTTTATTTTGTATGAGTATGTTCAAATATTTTGATTTTAGGTTGTTTCTATAGAAAATTTGCTGCCTACACGAAAAGATTGAAAACCTTCTACCGAAGTAGAATACCCTAATTGGAAGGAAAGTATTGGTCATCCTATTTACTATTTGTTAAAATATCAAACTAGTGATTTTCTTTTATAGAGTTCAACTTTTTGATACACCGAAAATGAATTTACTTGGTAGATCACAATCAATTAGGTGTGATGTTAGATGTAATTAAGTGAATCGTTTTCAATAATTTTACGATTATGTTTGATTTTATAAATATATATTGATATTGTATATCAATTAAATCTATTAAGTCTTCAACTTCTACGCATTGACATTATAGAATTGTTTTACATATAATCAATTAAATTACATCATATAAACATTTGTAGAGATATTTTATAAAATGCCCTAAGAAAGTGTATTATAATTTGATTTGATTGTATGCACGTGTAAACCAATTTTTTAACGTCGATGTCCGTGCATAATTGTAAAGATGTGTTATGTAAAGATGATTTACTTTTAGTGTTCGGATGGTAGATCACAATCAATTATTTTAGTGTTCAATGGTTTACTTTTGGAAAGATGTTTTTTCCCTCAAATATTAGCACGTTTTCTTTTCTAGGTTTTATCTTAACACTTTGGCAATGAGAAAATGCTAATTACCATGGTTTTTGAATTTGCAGGGTTTCCGAAAGCTTGTGCCAGATAAATGGGAATTTGCAAACGAAAATTTCAAACGAGGCCACAAACACCTCCTCACCGATATCAAACGCCGCAAAACCATCTCACAACCATCGTCACAACCCCTGGAAGCTGAAAAAACCAGCATCAATGACAACTCTCCATCAAACTCCGGCAACGACGGAGCAGGATCCACCTCAACCTCTTCCTCCAACTCAAAAAACCCTGGATCAGTGGAAACAACAACACTTCCTCAATGTGTTAACTTATCGAGCGAAAATGAGAAACTAAAAAAAGACAACGAAACCTTGAGTTGTGAATTGGCACGTGCAAAGAAACAATGCGAAGAGTTGGTGACTTTTCTAAAAGACAATTTAAATGTGGGTCCAGATCAAATCGATCGCATAATGAAGCAACAAGAAAATTGTGAATCTGTTCAAAATGCGGTTGGTGAAGTTGAAGAAAGTGGAAGAGATTGTTTGAAATTGTTTGGTGTTTGGTTGAAAGAAGAAAATTACACAAAAGAAAGAAACAATAAGAAGAGAGGTTGTGAAGACCAATTGAGCTTTGGTGGGCCTCGAGCTAAAGTGGGTAACAATGCTGTTGACTTTAGTGCTGTTAATGTTGTGAACATGATGAAAAGTGGCAAGGTTTGTAACTGA
Protein sequence

>MS.gene047640.t1

MSQRSAPAPFLLKTYQMVDDFATDDVISWSESGESFVVWKHADFARDLLPKYFKHNNFSSFVRQLNTYGFRKLVPDKWEFANENFKRGHKHLLTDIKRRKTISQPSSQPLEAEKTSINDNSPSNSGNDGAGSTSTSSSNSKNPGSVETTTLPQCVNLSSENEKLKKDNETLSCELARAKKQCEELVTFLKDNLNVGPDQIDRIMKQQENCESVQNAVGEVEESGRDCLKLFGVWLKEENYTKERNNKKRGCEDQLSFGGPRAKVGNNAVDFSAVNVVNMMKSGKVCN